MD11G1034400.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000070
Physical Location & Seq
Reverse (-)
3035639 .. 3041518
5880 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1034400.v1.1.491

Sequence Viewer

Length: 4020 bp
ATGGCACTGGGAGAGATTTTTCTTGCGGCGTTTCTACAGTTGCTGCTCGACAGGTTGACCCCTCGCGAGATTCTCAACTACTTTGGAAACTTCCGGGGCGTCAGAAGGAAGCTGGAGAAGTTGAGGACCACGTTATCTACAATCGGAGTGGTGGTGAGTGATGCCGAGGAAAGGCAACTGACTGAGGGTGCTGTAAAACTGTGGCTCGATGATCTCAGAGATTTGGCTTATGATATCGAAGACACGTTGGACAAATTTGCCACACAAATGTTGAAGCGCATGATAGAGGGACGTGAACAAGCCAACACAAGCAACAAGATACGGAGATCATTTTATAAAGTTAAATTGAGGTTTGATATGAACTCCGAAATGAAGAAGATTATGGAGCGGTTGCAAGACATATCTGAACGGAAAGAAAAGTTTGGCTTGAAAGATACTGGGACGTCTGCTAAGGAATCACGAAGTTTACAAAGTGCAGGCGTGTTAGATGAAAAGCTTGTTGTTGGAAGAGATGATGACAAAGGGGAGATTATCGAATTGTTGTCAAGAAAATACAAGCATGCAGGTACTGTCAATTTTGGTGTAGTTGCTATAGTTGGGATGCCCGGAGTCGGGAAGACGACACTTGCTCAACTTGTATTCAACCACAAAGATGATGCCATGAAGGAGTTTGAGCCAAAGGTGTGGGTATCTGTGTCTGATGACTTCGATGTTGTGCGAGTGACAAAGGCAATTCTTGAATCAATCACATCCCGACCCGTTCAAGTCGAGGAGTTTAGTAAAATGCAGCATGATTTGAGTGAGCAGTTAAGAGGAAAAAAGTTTTTAATCGTTTTAGATGATATTTGGAACAAAGGTGACTATGATCTATACGATCTCTGGACAAGACTTCAATCCCCTTTTGGCGTCGGAGCTGGAGGAAGTAAGATACTTGTGACAACCCGTGATGTGAATGTTGCCAAGTTTATGGGAGCCGCTGGAGTCCATAATTTGAAGTGCATGAGAGATGATGATTGTTTGGAAATATTTGAGCGACATGCATTCGGGGAACTTAACGATGGAAAACCAGTAAATTATGAGTTAATTCGAAGAAAAATTGTCGAAAAATGTCGTGGATTACCATTTGCTGCAAGGACTCTCGGTGGCCTTTTACGTTGCAAAGAAAAAGATGAGTGGGAAGAAATATTGAACAACAAATTGTGGAATATAGCAGATAAGAGTGACATTCTCCCCGTACTAAAGTTGAGCTATCACTATCTTCCATCAAATTTGAAGAGGTGTTTTGCCTATTGCTCAATACTTCCAAACGACTATGAATTTAGGGAGAAGCAACTCGTCCTTTTGTGGATGGCAGAGGGTTTGATTCAACAAAAACCTGAAGACAATAAACAAATGGAGGATTTGGGCCGTGACTACTTTCGAGAGCTATTATCAAGGTCGTTGTTTCAAGAATCAAGCAAAAACAATTCACGATTTGTAATGCATGACCTCGTTAATGATTTAGCACAATGGGCAGCAGGAGAAATATGTTCTAGGTTGGAAGTTACCAGATTTGAGGACTTTCCTAAAGTTGAACGTTTGCGAACATTCCTGCCACTATCGCTTTCAGATTCCAGGTGGTGGAGTAAATATTTGTCTCATAAGGTTATTTTTGAGTTACTACCACAGTTGCAATACTTACGAGTGCTCTCTTTGAATGGCTACAAAATAACTGAGCTGCCAAACTCAATTGGTGATTTGAGGTTGTTACAGTATCTTGACCTTTCCTACACAGATATAACCAGTTTGCCTGAATCAACAAGCACTCTTTACAGCTTGCAAACATTGATATTGGAAGGTTGTTCTAAATTGAAGTCATTGCCTGCGAAGATGAGTAATCTAATTAATTTGCGCCATCTAAACAACTTGGATGCATATTCGTTGGGAGGAATGCCTCCACAACTGGGTAGAATGGTGAATCTCCAATCATTGTCTAATTTTGTGGTCAGTGGTGGTAGTGATGGATCAAGGATAAGGGAGATAGAGTTCCTATTGCATCTCCGCGGGACATTGTGCATCTCAAGATTGGAGAATGTGATTGATGTCGAGGATGCTCGGAGGGCCAACTTAAAATGCAAGGAGAGGCTTGATTCATTGGTCCTAGAATGGTCTCATTCAAGCAACACAAGAGAAACGGAATCTGCTGTGCTTGACATGTTACAGCCTCATACAAAGCTCAAGGAGCTCACCATCAAGAAACTTTATATTAGAGGAATGAATGCAGTGGAAAGTGTTGGTGCTGAGTTTTATGGAGAGTGCATCTCGCCTTTTCCGCTATTAGAGACTCTTGAGTTTGTGGATATGCAACATTGGAAGGAATGGCTTCCTTTCCAATTGGATCGTGGAAATGGTGTTTTCCCTTGCCTGAAAACGCTCTTTGTAAAAAAATGTTCAAAACTGGGGGGTAAGTTGCCAGAGAACCTCAATTCTTTAGCAAAGCTTGGAATTGTTAAATGTGAAGAATTAGTGGTTTCAATTGCCAACCACAAACAACTTCGTCATTTAAACATTGACTGTTGTAAAGTGTTGGTGCATACAGCTGCTAAGGTTGAGTTTGAGTTGTTAGAGTCCTTGTGCCTTTCAAACATTTCGGAGCTCATGTCTCTGCAAACAGGGGAATTGTTCCAGAAGGGATTAACCAAGGTTAGAGATTTGAAGATTAATGGATGTGAGGAGCTGATGTCTTCATTGAAGAACGAGGGTAGATTATTGCAACAGTTGACTTCTCTTGGCCGTTTGGAAATTGAAGACAACTCTCCTCTAGTTGAAGAATTGGGAAAAGAAGCAGAGGAGTTGCTGCAATTGCAAATATTGGAGTGCAAGCTTGAATGTCTGGAGTTAAAGAAGTGCGAAAATATTTTGAAGCTACCAAAAGGGTTAAATCAGTTGTCGTCTCTTCAACAGCTTTGCATACACAAATGTCCACGTCTGGTTTCTTTTGAGGATGTTGGTCTGCCACCTTCTCTTAAAGACATCAAGATTACAGAGTGTCATTCGTTGATATATTTGGCAAAATTTCAGGTTCCCCAAAATCTCAGAAGAATACAGATAAGTGACTGTAAAAGTTTGAAATCACTAGTAGATGAGGAGGTTGTTGGTTCTTCTCTGTTGTCTTCTCACATTTGCCTTGAGTACTTGAAAATCCAGGAATGTCAATCTCTGACGTCGTTATCATTGAGCGGCCAGCTTCTTAGGACACTTAAACACCTTGAGATATACGATTGTTTACAGCTGGAGTTAATTGTACAGGACGGGTTCTCCCACTACAATACTAATCAGTGTCTTGAATATATAAGGATCTGGAAGTGCCAAAATCTGAAATACTTACTAGATGGCTTATACCACCTCATCAATCTTCAAACGTTGGATATTTATTACTGTGGAAGTCTTGTTTCTATCCCCAGACTAAGTGGGGGGAGAAAAGCCTCCAACCTGAGAGAGATCAAGATAACCGATTGCGACAAATTGGAGGTGTTACCCGAAGCCATGCGCAATCTCAACTCTCTTGAGGAATTGACCATTGACTACCGTGAAGGTTTGAGTTGCTTCTTTCCCGCCAACCTCACATCACTTACAATTTCGAAAGTCAAGAGCTGTAAGTCATTGTGGGAGTTGGAGTGGGGATTGCACAGACTCACCTCTCTTAGAAAATTATGGATCTACGGCAATGACCCGGATATGGTATCGTTTCCACCCGACGTGGTCCAGACGGAGACACTCCTCCCCAAATCTCTCATTGGACTATTAATTTGTGGTTTCCCGAATCTGATGAAACTGAGCAGCAAAGGCTTTCAATCCCTAACGTCTCTTCAATCTCTACATCTCTCCAGTTGTTTTCGACTAGCATCCATTCCAGAAGAGGGTCTTCCTCCTTCACTTACACAACTTCTCATCTCTAGGTGTCCTGTGCTAAAAGAGAGATGCGAACGAGGAAAAGGACGCTACTGGCACAAAATATCCCACATCCCTTACATAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1340

Amino Acids

152.94

Weight (kDa)

7.25

Isoelectric Point (pI)

49.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 11 - 99 2.4e-21 Rx N-terminal domain
NB-ARC PF00931 173 - 348 7.9e-37 NB-ARC domain
WHD_DRP PF23559 433 - 502 1.4e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 554 - 712 1e-15 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 671 - 810 3.4e-20 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 1053 - 1167 2.1e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 336
AatII GACGTC 2 cut(s) 446, 3206
Acc16I TGCGCA 1 cut(s) 3530
Acc36I ACCTGC 1 cut(s) 554
AccB7I CCANNNNNTGG 1 cut(s) 1620
AccBSI CCGCTC 2 cut(s) 388, 3219
AccII CGCG 2 cut(s) 66, 2043
AciI CCGC 8 cut(s) 26, 388, 975, 2041, 2043, 2312, 3219, 3594
AclI AACGTT 2 cut(s) 1576, 3401
AclWI GGATC 4 cut(s) 2011, 2385, 3344, 3704
AcoI YGGCCR 2 cut(s) 2770, 3220
AcsI RAATTY 4 cut(s) 254, 1267, 1316, 3053
AcuI CTGAAG 1 cut(s) 1398
AcyI GRCGYC 4 cut(s) 99, 443, 906, 3203
AfaI GTAC 4 cut(s) 568, 1236, 3173, 3285
AfiI CCNNNNNNNGG 7 cut(s) 171, 611, 612, 1620, 1943, 3718, 3899
AflIII ACRYGT 2 cut(s) 243, 2193
AhlI ACTAGT 1 cut(s) 3115
AjiI CACGTC 3 cut(s) 293, 2966, 3739
AjnI CCWGG 2 cut(s) 1613, 3183
AloI GAACNNNNNNTCC 2 cut(s) 2009, 2041
Alw21I GWGCWC 3 cut(s) 1689, 2226, 2637
Alw26I GTCTC 7 cut(s) 1641, 2154, 2315, 2646, 2937, 3746, 3849
AlwI GGATC 4 cut(s) 2011, 2385, 3344, 3704
AlwNI CAGNNNCTG 2 cut(s) 43, 569
AoxI GGCC 5 cut(s) 1144, 1405, 2100, 2770, 3220
ApeKI GCWGC 8 cut(s) 43, 787, 1127, 1514, 1717, 2579, 2836, 3819
ApoI RAATTY 4 cut(s) 254, 1267, 1316, 3053
ArsI GACNNNNNNTTYG 2 cut(s) 3771, 3803
AseI ATTAAT 3 cut(s) 1884, 2700, 3785
Asp700I GAANNNNTTC 1 cut(s) 2361
AspLEI GCGC 3 cut(s) 279, 1893, 3531
AspS9I GGNCC 5 cut(s) 126, 1405, 2100, 2137, 3742
AsuC2I CCSGG 3 cut(s) 95, 606, 3713
AsuHPI GGTGA 6 cut(s) 166, 869, 1745, 1966, 2218, 3667
AsuII TTCGAA 2 cut(s) 1087, 3620
AvaII GGWCC 3 cut(s) 126, 2137, 3742
BanII GRGCYC 2 cut(s) 2226, 2637
BbsI GAAGAC 7 cut(s) 246, 623, 1386, 2715, 2793, 3144, 3896
Bbv12I GWGCWC 3 cut(s) 1689, 2226, 2637
BbvI GCAGC 8 cut(s) 30, 799, 1114, 1526, 1704, 2566, 2823, 3831
BccI CCATC 7 cut(s) 1052, 1270, 1342, 1902, 1994, 2237, 3365
BceAI ACGGC 3 cut(s) 1392, 2757, 3718
BciT130I CCWGG 2 cut(s) 1615, 3185
BcnI CCSGG 3 cut(s) 95, 606, 3713
BcoDI GTCTC 7 cut(s) 1641, 2154, 2315, 2646, 2937, 3746, 3849
BcuI ACTAGT 1 cut(s) 3115
BfaI CTAG 7 cut(s) 1533, 2141, 2801, 3116, 3368, 3881, 3936
BfmI CTRYAG 2 cut(s) 35, 591
BfuAI ACCTGC 1 cut(s) 554
BmcAI AGTACT 1 cut(s) 3173
Bme1390I CCNGG 5 cut(s) 95, 606, 1615, 3185, 3713
Bme18I GGWCC 3 cut(s) 126, 2137, 3742
BmgBI CACGTC 3 cut(s) 293, 2966, 3739
BmgT120I GGNCC 5 cut(s) 126, 1405, 2100, 2137, 3742
BmiI GGNNCC 2 cut(s) 973, 3063
BmrFI CCNGG 5 cut(s) 95, 606, 1615, 3185, 3713
BmrI ACTGGG 4 cut(s) 17, 447, 1952, 2447
BmuI ACTGGG 4 cut(s) 17, 447, 1952, 2447
BpiI GAAGAC 7 cut(s) 246, 623, 1386, 2715, 2793, 3144, 3896
BplI GAGNNNNNCTC 2 cut(s) 2285, 2317
BpmI CTGGAG 6 cut(s) 134, 936, 999, 2894, 3293, 3850
Bpu10I CCTNAGC 2 cut(s) 450, 2583
Bpu14I TTCGAA 2 cut(s) 1087, 3620
BpuEI CTTGAG 6 cut(s) 2044, 2201, 2348, 3188, 3269, 3566
BpuMI CCSGG 3 cut(s) 95, 606, 3713
BsaHI GRCGYC 4 cut(s) 99, 443, 906, 3203
BsaI GGTCTC 1 cut(s) 2154
BsaJI CCNNGG 4 cut(s) 94, 165, 2041, 2679
Bsc4I CCNNNNNNNGG 7 cut(s) 171, 611, 612, 1620, 1943, 3718, 3899
Bse1I ACTGG 8 cut(s) 12, 442, 1067, 1782, 1947, 2442, 3867, 3989
Bse3DI GCAATG 2 cut(s) 1856, 3712
BseBI CCWGG 2 cut(s) 1615, 3185
BseDI CCNNGG 4 cut(s) 94, 165, 2041, 2679
BseGI GGATG 9 cut(s) 606, 749, 1353, 1915, 2095, 2711, 2989, 3884, 4002
BseLI CCNNNNNNNGG 7 cut(s) 171, 611, 612, 1620, 1943, 3718, 3899
BseMI GCAATG 2 cut(s) 1856, 3712
BseMII CTCAG 7 cut(s) 174, 229, 1704, 2271, 3088, 3464, 3806
BseNI ACTGG 8 cut(s) 12, 442, 1067, 1782, 1947, 2442, 3867, 3989
BseRI GAGGAG 6 cut(s) 785, 2726, 2787, 2843, 3140, 3749
BseXI GCAGC 8 cut(s) 30, 799, 1114, 1526, 1704, 2566, 2823, 3831
BsgI GTGCAG 1 cut(s) 495
Bsh1236I CGCG 2 cut(s) 66, 2043
BshFI GGCC 5 cut(s) 1146, 1407, 2102, 2772, 3222
BsiHKAI GWGCWC 3 cut(s) 1689, 2226, 2637
BsiSI CCGG 3 cut(s) 94, 606, 3713
BslFI GGGAC 3 cut(s) 303, 454, 2059
BslI CCNNNNNNNGG 7 cut(s) 171, 611, 612, 1620, 1943, 3718, 3899
BsmAI GTCTC 7 cut(s) 1641, 2154, 2315, 2646, 2937, 3746, 3849
BsmBI CGTCTC 2 cut(s) 2937, 3849
BsmFI GGGAC 3 cut(s) 303, 454, 2059
BsmI GAATGC 3 cut(s) 1040, 1935, 2263
BsnI GGCC 5 cut(s) 1146, 1407, 2102, 2772, 3222
Bso31I GGTCTC 1 cut(s) 2154
Bsp119I TTCGAA 2 cut(s) 1087, 3620
Bsp1286I GDGCHC 3 cut(s) 1689, 2226, 2637
Bsp1407I TGTACA 1 cut(s) 3283
Bsp143I GATC 9 cut(s) 211, 326, 865, 874, 2003, 2377, 3336, 3480, 3696
Bsp68I TCGCGA 1 cut(s) 66
BspACI CCGC 8 cut(s) 26, 388, 975, 2041, 2043, 2312, 3219, 3594
BspANI GGCC 5 cut(s) 1146, 1407, 2102, 2772, 3222
BspCNI CTCAG 7 cut(s) 175, 228, 1705, 2272, 3087, 3465, 3807
BspFNI CGCG 2 cut(s) 66, 2043
BspLI GGNNCC 2 cut(s) 973, 3063
BspMI ACCTGC 1 cut(s) 554
BspPI GGATC 4 cut(s) 2011, 2385, 3344, 3704
BspT104I TTCGAA 2 cut(s) 1087, 3620
BspTNI GGTCTC 1 cut(s) 2154
BsrBI CCGCTC 2 cut(s) 388, 3219
BsrDI GCAATG 2 cut(s) 1856, 3712
BsrGI TGTACA 1 cut(s) 3283
BsrI ACTGG 8 cut(s) 12, 442, 1067, 1782, 1947, 2442, 3867, 3989
BssECI CCNNGG 4 cut(s) 94, 165, 2041, 2679
BssMI GATC 9 cut(s) 211, 326, 865, 874, 2003, 2377, 3336, 3480, 3696
BssNI GRCGYC 4 cut(s) 99, 443, 906, 3203
BssT1I CCWWGG 1 cut(s) 2679
Bst2UI CCWGG 2 cut(s) 1615, 3185
Bst6I CTCTTC 5 cut(s) 502, 1268, 2940, 3852, 3891
BstACI GRCGYC 4 cut(s) 99, 443, 906, 3203
BstAUI TGTACA 1 cut(s) 3283
BstBI TTCGAA 2 cut(s) 1087, 3620
BstC8I GCNNGC 6 cut(s) 478, 561, 1817, 1863, 2861, 3224
BstDSI CCRYGG 1 cut(s) 2041
BstF5I GGATG 9 cut(s) 606, 749, 1353, 1915, 2095, 2711, 2989, 3884, 4002
BstFNI CGCG 2 cut(s) 66, 2043
BstHHI GCGC 3 cut(s) 279, 1893, 3531
BstKTI GATC 9 cut(s) 214, 329, 868, 877, 2006, 2380, 3339, 3483, 3699
BstMAI GTCTC 7 cut(s) 1641, 2154, 2315, 2646, 2937, 3746, 3849
BstMBI GATC 9 cut(s) 211, 326, 865, 874, 2003, 2377, 3336, 3480, 3696
BstMWI GCNNNNNNNGC 7 cut(s) 1511, 1600, 2099, 2221, 2311, 2842, 3825
BstNI CCWGG 2 cut(s) 1615, 3185
BstNSI RCATGY 3 cut(s) 563, 1040, 2197
BstSCI CCNGG 5 cut(s) 93, 604, 1613, 3183, 3711
BstSFI CTRYAG 2 cut(s) 35, 591
BstUI CGCG 2 cut(s) 66, 2043
BstV1I GCAGC 8 cut(s) 30, 799, 1114, 1526, 1704, 2566, 2823, 3831
BstV2I GAAGAC 7 cut(s) 246, 623, 1386, 2715, 2793, 3144, 3896
BstX2I RGATCY 2 cut(s) 3336, 3696
BstXI CCANNNNNNTGG 2 cut(s) 684, 967
BstYI RGATCY 2 cut(s) 3336, 3696
BsuRI GGCC 5 cut(s) 1146, 1407, 2102, 2772, 3222
BtgI CCRYGG 1 cut(s) 2041
BtrI CACGTC 3 cut(s) 293, 2966, 3739
BtsCI GGATG 9 cut(s) 606, 749, 1353, 1915, 2095, 2711, 2989, 3884, 4002
BtsI GCAGTG 1 cut(s) 2268
BtsIMutI CAGTG 4 cut(s) 5, 1993, 2268, 3323
BtuMI TCGCGA 1 cut(s) 66
BveI ACCTGC 1 cut(s) 554
Cac8I GCNNGC 6 cut(s) 478, 561, 1817, 1863, 2861, 3224
CaiI CAGNNNCTG 2 cut(s) 43, 569
CfoI GCGC 3 cut(s) 279, 1893, 3531
Cfr13I GGNCC 5 cut(s) 126, 1405, 2100, 2137, 3742
Cfr42I CCGCGG 1 cut(s) 2044
CseI GACGC 3 cut(s) 88, 895, 3987
Csp6I GTAC 4 cut(s) 567, 1235, 3172, 3284
CspCI CAANNNNNGTGG 2 cut(s) 129, 164
CviQI GTAC 4 cut(s) 567, 1235, 3172, 3284
DpnI GATC 9 cut(s) 213, 328, 867, 876, 2005, 2379, 3338, 3482, 3698
DpnII GATC 9 cut(s) 211, 326, 865, 874, 2003, 2377, 3336, 3480, 3696
DraI TTTAAA 1 cut(s) 2544
EaeI YGGCCR 2 cut(s) 2770, 3220
Eam1104I CTCTTC 5 cut(s) 502, 1268, 2940, 3852, 3891
EarI CTCTTC 5 cut(s) 502, 1268, 2940, 3852, 3891
Ecl136II GAGCTC 2 cut(s) 2224, 2635
Eco130I CCWWGG 1 cut(s) 2679
Eco24I GRGCYC 2 cut(s) 2226, 2637
Eco31I GGTCTC 1 cut(s) 2154
Eco32I GATATC 1 cut(s) 235
Eco47I GGWCC 3 cut(s) 126, 2137, 3742
Eco53kI GAGCTC 2 cut(s) 2224, 2635
Eco57I CTGAAG 1 cut(s) 1398
EcoICRI GAGCTC 2 cut(s) 2224, 2635
EcoRII CCWGG 2 cut(s) 1613, 3183
EcoRV GATATC 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 2679
EcoT22I ATGCAT 3 cut(s) 1042, 1485, 1915
EcoT38I GRGCYC 2 cut(s) 2226, 2637
ErhI CCWWGG 1 cut(s) 2679
Esp3I CGTCTC 2 cut(s) 2937, 3849
FalI AAGNNNNNCTT 4 cut(s) 410, 442, 3888, 3920
FaqI GGGAC 3 cut(s) 303, 454, 2059
FauI CCCGC 2 cut(s) 2036, 3601
FokI GGATG 9 cut(s) 613, 736, 1360, 1922, 2102, 2718, 2996, 3871, 3989
FriOI GRGCYC 2 cut(s) 2226, 2637
FspBI CTAG 7 cut(s) 1533, 2141, 2801, 3116, 3368, 3881, 3936
FspI TGCGCA 1 cut(s) 3530
GlaI GCGC 3 cut(s) 278, 1892, 3530
GsuI CTGGAG 6 cut(s) 134, 936, 999, 2894, 3293, 3850
HaeIII GGCC 5 cut(s) 1146, 1407, 2102, 2772, 3222
HapII CCGG 3 cut(s) 94, 606, 3713
HgaI GACGC 3 cut(s) 88, 895, 3987
HhaI GCGC 3 cut(s) 279, 1893, 3531
Hin1I GRCGYC 4 cut(s) 99, 443, 906, 3203
Hin6I GCGC 3 cut(s) 277, 1891, 3529
HinP1I GCGC 3 cut(s) 277, 1891, 3529
HincII GTYRAC 2 cut(s) 57, 2760
HindII GTYRAC 2 cut(s) 57, 2760
HindIII AAGCTT 3 cut(s) 494, 2477, 2861
HpaII CCGG 3 cut(s) 94, 606, 3713
HphI GGTGA 6 cut(s) 166, 869, 1745, 1966, 2218, 3667
Hpy166II GTNNAC 6 cut(s) 57, 296, 467, 2760, 2963, 3266
Hpy8I GTNNAC 6 cut(s) 57, 296, 467, 2760, 2963, 3266
Hpy99I CGWCG 3 cut(s) 911, 3208, 3740
HpyAV CCTTC 8 cut(s) 99, 658, 1829, 2347, 2662, 3009, 3566, 3921
HpyF10VI GCNNNNNNNGC 7 cut(s) 1511, 1600, 2099, 2221, 2311, 2842, 3825
Hsp92I GRCGYC 4 cut(s) 99, 443, 906, 3203
HspAI GCGC 3 cut(s) 277, 1891, 3529
KspI CCGCGG 1 cut(s) 2044
Kzo9I GATC 9 cut(s) 211, 326, 865, 874, 2003, 2377, 3336, 3480, 3696
LmnI GCTCC 6 cut(s) 385, 911, 971, 2221, 2632, 2713
Lsp1109I GCAGC 8 cut(s) 30, 799, 1114, 1526, 1704, 2566, 2823, 3831
MaeI CTAG 7 cut(s) 1533, 2141, 2801, 3116, 3368, 3881, 3936
MalI GATC 9 cut(s) 213, 328, 867, 876, 2005, 2379, 3338, 3482, 3698
MbiI CCGCTC 2 cut(s) 388, 3219
MboI GATC 9 cut(s) 211, 326, 865, 874, 2003, 2377, 3336, 3480, 3696
MfeI CAATTG 4 cut(s) 1728, 2372, 2514, 2840
MflI RGATCY 2 cut(s) 3336, 3696
MhlI GDGCHC 3 cut(s) 1689, 2226, 2637
MlyI GAGTC 6 cut(s) 618, 990, 1129, 2317, 2615, 3666
MmeI TCCRAC 7 cut(s) 228, 484, 889, 1518, 3384, 3492, 3633
Mph1103I ATGCAT 3 cut(s) 1042, 1485, 1915
MroXI GAANNNNTTC 1 cut(s) 2361
MslI CAYNNNNRTG 2 cut(s) 266, 651
MspA1I CMGCKG 4 cut(s) 977, 2043, 2579, 3271
MspI CCGG 3 cut(s) 94, 606, 3713
MspR9I CCNGG 5 cut(s) 95, 606, 1615, 3185, 3713
MunI CAATTG 4 cut(s) 1728, 2372, 2514, 2840
Mva1269I GAATGC 3 cut(s) 1040, 1935, 2263
MvaI CCWGG 2 cut(s) 1615, 3185
MvnI CGCG 2 cut(s) 66, 2043
MwoI GCNNNNNNNGC 7 cut(s) 1511, 1600, 2099, 2221, 2311, 2842, 3825
NciI CCSGG 3 cut(s) 95, 606, 3713
NdeII GATC 9 cut(s) 211, 326, 865, 874, 2003, 2377, 3336, 3480, 3696
NlaIV GGNNCC 2 cut(s) 973, 3063
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 6 cut(s) 721, 857, 934, 1220, 1409, 3092
NruI TCGCGA 1 cut(s) 66
NsbI TGCGCA 1 cut(s) 3530
NsiI ATGCAT 3 cut(s) 1042, 1485, 1915
NspI RCATGY 3 cut(s) 563, 1040, 2197
NspV TTCGAA 2 cut(s) 1087, 3620
PaeI GCATGC 1 cut(s) 563
PciI ACATGT 1 cut(s) 2193
PctI GAATGC 3 cut(s) 1040, 1935, 2263
PdmI GAANNNNTTC 1 cut(s) 2361
PflFI GACNNNGTC 1 cut(s) 3740
PflMI CCANNNNNTGG 1 cut(s) 1620
PfoI TCCNGGA 1 cut(s) 3183
PleI GAGTC 6 cut(s) 617, 989, 1129, 2317, 2614, 3666
PpsI GAGTC 6 cut(s) 617, 989, 1129, 2317, 2614, 3666
PscI ACATGT 1 cut(s) 2193
PshBI ATTAAT 3 cut(s) 1884, 2700, 3785
PsiI TTATAA 1 cut(s) 336
Psp124BI GAGCTC 2 cut(s) 2226, 2637
Psp1406I AACGTT 2 cut(s) 1576, 3401
Psp6I CCWGG 2 cut(s) 1613, 3183
PspGI CCWGG 2 cut(s) 1613, 3183
PspN4I GGNNCC 2 cut(s) 973, 3063
PspPI GGNCC 5 cut(s) 126, 1405, 2100, 2137, 3742
PstNI CAGNNNCTG 2 cut(s) 43, 569
PsuI RGATCY 2 cut(s) 3336, 3696
PsyI GACNNNGTC 1 cut(s) 3740
PvuII CAGCTG 2 cut(s) 2579, 3271
RruI TCGCGA 1 cut(s) 66
RsaI GTAC 4 cut(s) 568, 1236, 3173, 3285
RsaNI GTAC 4 cut(s) 567, 1235, 3172, 3284
RseI CAYNNNNRTG 2 cut(s) 266, 651
SacI GAGCTC 2 cut(s) 2226, 2637
SacII CCGCGG 1 cut(s) 2044
Sau3AI GATC 9 cut(s) 211, 326, 865, 874, 2003, 2377, 3336, 3480, 3696
Sau96I GGNCC 5 cut(s) 126, 1405, 2100, 2137, 3742
ScaI AGTACT 1 cut(s) 3173
SchI GAGTC 6 cut(s) 618, 990, 1129, 2317, 2615, 3666
ScrFI CCNGG 5 cut(s) 95, 606, 1615, 3185, 3713
SduI GDGCHC 3 cut(s) 1689, 2226, 2637
SfcI CTRYAG 2 cut(s) 35, 591
Sfr303I CCGCGG 1 cut(s) 2044
SfuI TTCGAA 2 cut(s) 1087, 3620
SgrBI CCGCGG 1 cut(s) 2044
SinI GGWCC 3 cut(s) 126, 2137, 3742
SmiMI CAYNNNNRTG 2 cut(s) 266, 651
SmlI CTYRAG 6 cut(s) 2059, 2216, 2327, 3167, 3248, 3545
SmoI CTYRAG 6 cut(s) 2059, 2216, 2327, 3167, 3248, 3545
SpeI ACTAGT 1 cut(s) 3115
SphI GCATGC 1 cut(s) 563
SsiI CCGC 8 cut(s) 26, 388, 975, 2041, 2043, 2312, 3219, 3594
SspI AATATT 5 cut(s) 1026, 1185, 1631, 2850, 2896
SspMI CTAG 7 cut(s) 1533, 2141, 2801, 3116, 3368, 3881, 3936
SstI GAGCTC 2 cut(s) 2226, 2637
StyD4I CCNGG 5 cut(s) 93, 604, 1613, 3183, 3711
StyI CCWWGG 1 cut(s) 2679
TatI WGTACW 2 cut(s) 3171, 3283
TauI GCSGC 3 cut(s) 29, 977, 3222
TscAI CASTG 4 cut(s) 12, 1993, 2268, 3323
TseFI GTSAC 6 cut(s) 721, 857, 934, 1220, 1409, 3092
TseI GCWGC 8 cut(s) 43, 787, 1127, 1514, 1717, 2579, 2836, 3819
Tsp45I GTSAC 6 cut(s) 721, 857, 934, 1220, 1409, 3092
TspDTI ATGAA 9 cut(s) 374, 386, 504, 677, 1329, 2121, 2270, 2715, 3824
TspGWI ACGGA 4 cut(s) 337, 424, 2189, 3764
TspRI CASTG 4 cut(s) 12, 1993, 2268, 3323
Tth111I GACNNNGTC 1 cut(s) 3740
Van91I CCANNNNNTGG 1 cut(s) 1620
VpaK11BI GGWCC 3 cut(s) 126, 2137, 3742
VspI ATTAAT 3 cut(s) 1884, 2700, 3785
XapI RAATTY 4 cut(s) 254, 1267, 1316, 3053
XceI RCATGY 3 cut(s) 563, 1040, 2197
XcmI CCANNNNNNNNNTGG 1 cut(s) 2378
XmnI GAANNNNTTC 1 cut(s) 2361
XspI CTAG 7 cut(s) 1533, 2141, 2801, 3116, 3368, 3881, 3936
ZraI GACGTC 2 cut(s) 444, 3204
ZrmI AGTACT 1 cut(s) 3173
Zsp2I ATGCAT 3 cut(s) 1042, 1485, 1915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.