Rmu_sc0023430.1_g000001

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0023430.1
OG0000070
Physical Location & Seq
Forward (+)
21 .. 14807
14787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0023430.1_g000001.1.cds

Sequence Viewer

Length: 3408 bp
atgcagaattatgactcatcgcgacgtagattggttcgtcattcgtcttacattcctggtaggtatgatgggattaaaaagtttggggtctattctgaagctatatgtttgcggacattcttaccactttcactttcaattaataattatctggctgaaagggttacttctgatttattgcccaaactccaatacttgcgactgctctctttgagtggatatagaataaccgagctgccaaatacaattggtaaattgaagcatctacggtatcttgatctttttcacactcgaataggaagtttgccagactcaacaaccactctttacaacttacaaacattgatattggacaaatgtgaccagttgcgggcactacccacaggcatgaggaatctagtaaatttgcgtcacctcatgaattcgtatacatcttcattggaagaaatgcctcctcagcttggtcgattgactaatctccaaacattgcctaattttgttattggcaaaggtagtggatcaggagtaagagagattgggtcattgttgcgtctgcaagggaaattgcacctcacaagactagagaatgtggcttgtgtcgaggatgctagaagtgccagcttaaagagcaaggaggggcttgaagctctgtttctttcgtggtcttcttcaagtgacttgacagaaattgcaaaccatgtgcttgacatgttacaacctcattccaaactcaaagagctcaccatccaaggttatgccggattgaaattttcaacatggattggagacccttcattctctaatatggtgcgtgtaaggttagaaggttgtcatcattgtcaattcttgccaccattcggacagttgccttctctcaaagaactctatatacaagaaatggatgcagtggaaagtgttggtattgagttttatggagaaggtagcttgccgtttcaagctttagagacccttgagtttcagaatctgaaaaattggaagaagtggtctgcttgccaacaaaatgagggagttggggttttctcttgcctgaaaaagctttccatcagcgactgccccaaattggagggtagtttaccggagaagcttgattcattagcacagcttcagatatctggatgtgaggaattggtggtttcaatatcccattataaacagctttatgaatcagacatcaaaaattgcaagatggtggtttacgacacaagtccagttctcttggacttactagagaggttgacattttcaaatgtttcagagctgagattcgaaagagaggcgtttatgaaaagcttgaaaaatgttaaagagttggagattactggttgtgaggagctgacatgttcctttcagaatgaggatagattactgcaacatttgatttctcttggtcgtttgtgtattgaagacaactctgcccttgttgaaaagttagggaaagaagcagagcagttggtgcaattgcaaatactggattgcaagcttgaacgtctgcaattaagcaagtgtggaagccttctgaaggtaccagaaggattgcatcaccttacatctcttcaagtgcttcgcataaagggatgttcaagtctagtttcttttccggatgttggtctgccaccttgtcttaaagtcatagacattcagaagtgcgattctctgttgcactttgcaaaataccagattcctccaagcctaaaaaatatagtgatttggggatgtgagaaattgaaatcattgatagagaaagaggaggtggtggtggatggttcttgttcgtcttattgtctcgagtatttgaccatagttaactgtccatctctgttgtccttattatgcaaaggccaattacccagggctcttaaacagctttggatagataattgtagacagctggagttaataactgagaggttcctcgatgacacctgtcaactcgaagaaattcatatcacaacatgcccaaaccttaaatccttacctgagggactgtgccacctcaccaatcttcagtcattatatatttggggttgtcaaagtctggtttcgttgccgagaatgagtgtgtggccaagagatatgactatcagagattgtgagaaattggaggtggcacacttgctgagagacatgatgcacactgagatattgtggattgattactgtgaaggtttgactactacttcctttccccccaacctaacatcacttgaaattaacaagatcaagaattgtaaggcgctgatggaatctcaagggttgcacagactcacctctttgagagaattgcggatctacggtggtggagatgatcgaggtctggtgtcctttccacctggtgagaattcgaaggagaaggagatgctgttccccagatctcttgttgtattgacaattgcaggcttcccaaatctaaagaaactgagcaagggctttcaattcctcacctctcttgagagtctccaaatcttcaactgtccgaagcttacaaccattccagaggagggcctgcctctttcacttacgacacttgtgatttctgggttggtttcgaggtctgcttatgctcaaaactgtagagcacatccttttcctcctccacagaatatgatatttggattctcaattccagcctttccaaatcaatcttcctgggtgcttcttggcagtgttatgagtgttaatctgacgggttcaaagtctttggttggagtagctggagttgcatcagagattcaaaaggagaagaaggggcgggacaggtatgttatgttgagctccttggtgggaattctggaagtttacttagtactgagtacgtgggctccgcaaatgttgggaaatcagcttttatccgtgctttactgggatccagttgctgcatcagcgcaaaagtacaaaccaatacaatctgctgttcctggaactaccttgggtccaattcaaatgaacgctttcttaggaggagggaaattatatgacacatctggagttcatatccaccataggcaagcagcagtggttcattcagaagacctcccttcccttgctccacaaagtcagcttaggggtcaatacatatgctttgttacagggcaccaagctttgaattctgggccataccctgtggtggtgagcactaggcctttcgtagaggctgtcagagctgcttggagggctgttgataatgggctttctgcagttgatgcagttgttgagcgctgttcggcttgtgaggaactcagatgtgatggtacagttgggcctggtgggattcctgatgagaatggagagacgacaatcgatgctctggtcatggatggggtgacgatggaggttggagcagttgctgccatgaggtatgtcaaagatggaatcaaagctgctgggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1135

Amino Acids

126.25

Weight (kDa)

6.6

Isoelectric Point (pI)

41.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1170
Acc65I GGTACC 1 cut(s) 1543
AccB1I GGYRCC 2 cut(s) 1543, 3111
AccI GTMKAC 2 cut(s) 428, 1903
AccII CGCG 1 cut(s) 22
AccIII TCCGGA 1 cut(s) 1618
AciI CCGC 5 cut(s) 112, 370, 2305, 2770, 2843
AclWI GGATC 4 cut(s) 526, 2315, 2879, 2892
AcoI YGGCCR 1 cut(s) 2084
AcsI RAATTY 7 cut(s) 403, 421, 767, 1959, 2359, 2805, 3124
AcuI CTGAAG 4 cut(s) 117, 1109, 1559, 2009
AdeI CACNNNGTG 1 cut(s) 2354
AfaI GTAC 5 cut(s) 1545, 2826, 2833, 2912, 3271
AfeI AGCGCT 1 cut(s) 3236
AfiI CCNNNNNNNGG 8 cut(s) 370, 461, 857, 1081, 1540, 1625, 2519, 3145
AflIII ACRYGT 2 cut(s) 708, 1358
AjnI CCWGG 6 cut(s) 55, 1868, 2350, 2666, 2935, 3280
AjuI GAANNNNNNNTTGG 2 cut(s) 2647, 2679
AloI GAACNNNNNNTCC 2 cut(s) 2366, 2398
Alw21I GWGCWC 4 cut(s) 741, 2599, 2795, 3155
Alw26I GTCTC 6 cut(s) 780, 959, 1808, 2136, 2480, 3302
AlwI GGATC 4 cut(s) 526, 2315, 2879, 2892
AlwNI CAGNNNCTG 4 cut(s) 985, 1071, 2894, 3365
Ama87I CYCGRG 1 cut(s) 1805
Aor13HI TCCGGA 1 cut(s) 1618
Aor51HI AGCGCT 1 cut(s) 3236
AoxI GGCC 6 cut(s) 1858, 2084, 2521, 3131, 3158, 3278
ApeKI GCWGC 6 cut(s) 235, 2894, 3029, 3182, 3365, 3398
ApoI RAATTY 7 cut(s) 403, 421, 767, 1959, 2359, 2805, 3124
AseI ATTAAT 1 cut(s) 141
Asp700I GAANNNNTTC 2 cut(s) 1959, 2969
Asp718I GGTACC 1 cut(s) 1543
AspLEI GCGC 3 cut(s) 2257, 2905, 3237
AspS9I GGNCC 4 cut(s) 2521, 2951, 3131, 3278
AsuHPI GGTGA 9 cut(s) 404, 733, 1553, 2008, 2278, 2366, 2452, 3160, 3352
AsuII TTCGAA 2 cut(s) 1287, 2363
AvaI CYCGRG 1 cut(s) 1805
AvaII GGWCC 1 cut(s) 2951
AxyI CCTNAGG 1 cut(s) 1998
BaeGI GKGCMC 2 cut(s) 376, 3114
BalI TGGCCA 1 cut(s) 2086
BamHI GGATCC 1 cut(s) 2884
BanI GGYRCC 2 cut(s) 1543, 3111
BanII GRGCYC 4 cut(s) 741, 1876, 2795, 2842
BbsI GAAGAC 3 cut(s) 657, 1431, 3054
Bbv12I GWGCWC 4 cut(s) 741, 2599, 2795, 3155
BbvCI CCTCAGC 1 cut(s) 456
BbvI GCAGC 6 cut(s) 222, 2881, 3041, 3169, 3352, 3385
BceAI ACGGC 1 cut(s) 934
BciT130I CCWGG 6 cut(s) 57, 1870, 2352, 2668, 2937, 3282
BcoDI GTCTC 6 cut(s) 780, 959, 1808, 2136, 2480, 3302
BfaI CTAG 6 cut(s) 398, 581, 609, 1247, 1607, 3156
BfmI CTRYAG 2 cut(s) 2590, 3213
BfoI RGCGCY 2 cut(s) 2258, 3238
BglII AGATCT 1 cut(s) 2390
BisI GCNGC 6 cut(s) 236, 2895, 3030, 3183, 3366, 3399
BlsI GCNGC 6 cut(s) 237, 2896, 3031, 3184, 3367, 3400
BmcAI AGTACT 1 cut(s) 2826
Bme1390I CCNGG 6 cut(s) 57, 1870, 2352, 2668, 2937, 3282
Bme18I GGWCC 1 cut(s) 2951
BmeT110I CYCGRG 1 cut(s) 1805
BmgT120I GGNCC 4 cut(s) 2521, 2951, 3131, 3278
BmiI GGNNCC 6 cut(s) 1545, 1931, 2841, 2886, 2952, 3113
BmrFI CCNGG 6 cut(s) 57, 1870, 2352, 2668, 2937, 3282
BmrI ACTGGG 1 cut(s) 2890
BmuI ACTGGG 1 cut(s) 2890
BoxI GACNNNNGTC 2 cut(s) 1224, 1944
BpiI GAAGAC 3 cut(s) 657, 1431, 3054
BpmI CTGGAG 3 cut(s) 1931, 2754, 3024
Bpu10I CCTNAGC 2 cut(s) 456, 3080
Bpu14I TTCGAA 2 cut(s) 1287, 2363
BpuEI CTTGAG 3 cut(s) 992, 2253, 2489
Bsa29I ATCGAT 1 cut(s) 3318
BsaAI YACGTR 1 cut(s) 2835
BsaI GGTCTC 2 cut(s) 780, 959
BsaJI CCNNGG 6 cut(s) 748, 1868, 1869, 2667, 2796, 2946
BsaWI WCCGGW 2 cut(s) 1096, 1618
BsaXI ACNNNNNCTCC 6 cut(s) 1020, 1050, 2366, 2396, 2824, 2854
Bsc4I CCNNNNNNNGG 8 cut(s) 370, 461, 857, 1081, 1540, 1625, 2519, 3145
Bse1I ACTGG 6 cut(s) 364, 1229, 1345, 1494, 2885, 2888
Bse21I CCTNAGG 1 cut(s) 1998
Bse3DI GCAATG 1 cut(s) 485
BseAI TCCGGA 1 cut(s) 1618
BseBI CCWGG 6 cut(s) 57, 1870, 2352, 2668, 2937, 3282
BseCI ATCGAT 1 cut(s) 3318
BseDI CCNNGG 6 cut(s) 748, 1868, 1869, 2667, 2796, 2946
BseLI CCNNNNNNNGG 8 cut(s) 370, 461, 857, 1081, 1540, 1625, 2519, 3145
BseMI GCAATG 1 cut(s) 485
BseMII CTCAG 9 cut(s) 470, 1271, 1914, 1989, 2129, 2148, 2429, 2819, 3271
BseNI ACTGG 6 cut(s) 364, 1229, 1345, 1494, 2885, 2888
BseRI GAGGAG 6 cut(s) 444, 1364, 1781, 2531, 2601, 2994
BseSI GKGCMC 2 cut(s) 376, 3114
BseXI GCAGC 6 cut(s) 222, 2881, 3041, 3169, 3352, 3385
BseYI CCCAGC 1 cut(s) 3401
Bsh1236I CGCG 1 cut(s) 22
BshFI GGCC 6 cut(s) 1860, 2086, 2523, 3133, 3160, 3280
BshNI GGYRCC 2 cut(s) 1543, 3111
BshVI ATCGAT 1 cut(s) 3318
BsiHKAI GWGCWC 4 cut(s) 741, 2599, 2795, 3155
BsiHKCI CYCGRG 1 cut(s) 1805
BsiSI CCGG 3 cut(s) 759, 1097, 1619
BslFI GGGAC 2 cut(s) 2016, 2786
BslI CCNNNNNNNGG 8 cut(s) 370, 461, 857, 1081, 1540, 1625, 2519, 3145
BsmAI GTCTC 6 cut(s) 780, 959, 1808, 2136, 2480, 3302
BsmBI CGTCTC 1 cut(s) 3302
BsmFI GGGAC 2 cut(s) 2016, 2786
BsnI GGCC 6 cut(s) 1860, 2086, 2523, 3133, 3160, 3280
Bso31I GGTCTC 2 cut(s) 780, 959
BsoBI CYCGRG 1 cut(s) 1805
Bsp119I TTCGAA 2 cut(s) 1287, 2363
Bsp1286I GDGCHC 8 cut(s) 376, 741, 1876, 2599, 2795, 2842, 3114, 3155
Bsp13I TCCGGA 1 cut(s) 1618
Bsp143I GATC 7 cut(s) 277, 518, 2238, 2307, 2326, 2390, 2884
Bsp68I TCGCGA 1 cut(s) 22
BspACI CCGC 5 cut(s) 112, 370, 2305, 2770, 2843
BspANI GGCC 6 cut(s) 1860, 2086, 2523, 3133, 3160, 3280
BspCNI CTCAG 9 cut(s) 469, 1272, 1915, 1990, 2130, 2149, 2430, 2820, 3270
BspDI ATCGAT 1 cut(s) 3318
BspEI TCCGGA 1 cut(s) 1618
BspFNI CGCG 1 cut(s) 22
BspHI TCATGA 1 cut(s) 417
BspLI GGNNCC 6 cut(s) 1545, 1931, 2841, 2886, 2952, 3113
BspMAI CTGCAG 1 cut(s) 3217
BspPI GGATC 4 cut(s) 526, 2315, 2879, 2892
BspT104I TTCGAA 2 cut(s) 1287, 2363
BspT107I GGYRCC 2 cut(s) 1543, 3111
BspTNI GGTCTC 2 cut(s) 780, 959
BsrDI GCAATG 1 cut(s) 485
BsrI ACTGG 6 cut(s) 364, 1229, 1345, 1494, 2885, 2888
BssECI CCNNGG 6 cut(s) 748, 1868, 1869, 2667, 2796, 2946
BssMI GATC 7 cut(s) 277, 518, 2238, 2307, 2326, 2390, 2884
BssNAI GTATAC 1 cut(s) 429
BssT1I CCWWGG 3 cut(s) 748, 2796, 2946
Bst1107I GTATAC 1 cut(s) 429
Bst2UI CCWGG 6 cut(s) 57, 1870, 2352, 2668, 2937, 3282
Bst4CI ACNGT 9 cut(s) 270, 864, 1829, 2007, 2180, 2315, 2492, 2591, 3274
Bst6I CTCTTC 1 cut(s) 1578
BstAPI GCANNNNNTGC 3 cut(s) 1474, 3221, 3365
BstBAI YACGTR 1 cut(s) 2835
BstBI TTCGAA 2 cut(s) 1287, 2363
BstC8I GCNNGC 8 cut(s) 372, 619, 947, 1012, 1499, 2416, 2525, 3027
BstENI CCTNNNNNAGG 1 cut(s) 1538
BstFNI CGCG 1 cut(s) 22
BstH2I RGCGCY 2 cut(s) 2258, 3238
BstHHI GCGC 3 cut(s) 2257, 2905, 3237
BstKTI GATC 7 cut(s) 280, 521, 2241, 2310, 2329, 2393, 2887
BstMAI GTCTC 6 cut(s) 780, 959, 1808, 2136, 2480, 3302
BstMBI GATC 7 cut(s) 277, 518, 2238, 2307, 2326, 2390, 2884
BstNI CCWGG 6 cut(s) 57, 1870, 2352, 2668, 2937, 3282
BstNSI RCATGY 3 cut(s) 712, 1362, 1977
BstPAI GACNNNNGTC 2 cut(s) 1224, 1944
BstSCI CCNGG 6 cut(s) 55, 1868, 2350, 2666, 2935, 3280
BstSFI CTRYAG 2 cut(s) 2590, 3213
BstSLI GKGCMC 2 cut(s) 376, 3114
BstUI CGCG 1 cut(s) 22
BstV1I GCAGC 6 cut(s) 222, 2881, 3041, 3169, 3352, 3385
BstV2I GAAGAC 3 cut(s) 657, 1431, 3054
BstX2I RGATCY 3 cut(s) 2307, 2390, 2884
BstYI RGATCY 3 cut(s) 2307, 2390, 2884
BstZ17I GTATAC 1 cut(s) 429
Bsu15I ATCGAT 1 cut(s) 3318
Bsu36I CCTNAGG 1 cut(s) 1998
BsuRI GGCC 6 cut(s) 1860, 2086, 2523, 3133, 3160, 3280
BsuTUI ATCGAT 1 cut(s) 3318
BtgZI GCGATG 1 cut(s) 3
BtsI GCAGTG 3 cut(s) 912, 2689, 3039
BtsIMutI CAGTG 4 cut(s) 912, 2154, 2689, 3039
BtuMI TCGCGA 1 cut(s) 22
Cac8I GCNNGC 8 cut(s) 372, 619, 947, 1012, 1499, 2416, 2525, 3027
CaiI CAGNNNCTG 4 cut(s) 985, 1071, 2894, 3365
CciI TCATGA 1 cut(s) 417
CfoI GCGC 3 cut(s) 2257, 2905, 3237
Cfr13I GGNCC 4 cut(s) 2521, 2951, 3131, 3278
ClaI ATCGAT 1 cut(s) 3318
CseI GACGC 2 cut(s) 398, 539
CsiI ACCWGGT 1 cut(s) 2350
Csp6I GTAC 5 cut(s) 1544, 2825, 2832, 2911, 3270
CspCI CAANNNNNGTGG 2 cut(s) 496, 531
CviQI GTAC 5 cut(s) 1544, 2825, 2832, 2911, 3270
DpnI GATC 7 cut(s) 279, 520, 2240, 2309, 2328, 2392, 2886
DpnII GATC 7 cut(s) 277, 518, 2238, 2307, 2326, 2390, 2884
DraIII CACNNNGTG 1 cut(s) 2354
EaeI YGGCCR 1 cut(s) 2084
Eam1104I CTCTTC 1 cut(s) 1578
EarI CTCTTC 1 cut(s) 1578
Ecl136II GAGCTC 2 cut(s) 739, 2793
Eco130I CCWWGG 3 cut(s) 748, 2796, 2946
Eco147I AGGCCT 1 cut(s) 3160
Eco24I GRGCYC 4 cut(s) 741, 1876, 2795, 2842
Eco31I GGTCTC 2 cut(s) 780, 959
Eco32I GATATC 1 cut(s) 1131
Eco47I GGWCC 1 cut(s) 2951
Eco47III AGCGCT 1 cut(s) 3236
Eco53kI GAGCTC 2 cut(s) 739, 2793
Eco57I CTGAAG 4 cut(s) 117, 1109, 1559, 2009
Eco81I CCTNAGG 1 cut(s) 1998
Eco88I CYCGRG 1 cut(s) 1805
EcoICRI GAGCTC 2 cut(s) 739, 2793
EcoNI CCTNNNNNAGG 1 cut(s) 1538
EcoO109I RGGNCCY 1 cut(s) 2521
EcoRI GAATTC 4 cut(s) 421, 2359, 2805, 3124
EcoRII CCWGG 6 cut(s) 55, 1868, 2350, 2666, 2935, 3280
EcoRV GATATC 1 cut(s) 1131
EcoT14I CCWWGG 3 cut(s) 748, 2796, 2946
EcoT38I GRGCYC 4 cut(s) 741, 1876, 2795, 2842
ErhI CCWWGG 3 cut(s) 748, 2796, 2946
Esp3I CGTCTC 1 cut(s) 3302
FalI AAGNNNNNCTT 2 cut(s) 151, 183
FaqI GGGAC 2 cut(s) 2016, 2786
FauI CCCGC 2 cut(s) 363, 2763
FauNDI CATATG 1 cut(s) 3095
FblI GTMKAC 2 cut(s) 428, 1903
Fnu4HI GCNGC 6 cut(s) 236, 2895, 3030, 3183, 3366, 3399
FriOI GRGCYC 4 cut(s) 741, 1876, 2795, 2842
Fsp4HI GCNGC 6 cut(s) 236, 2895, 3030, 3183, 3366, 3399
FspBI CTAG 6 cut(s) 398, 581, 609, 1247, 1607, 3156
GlaI GCGC 3 cut(s) 2256, 2904, 3236
GluI GCNGC 6 cut(s) 236, 2895, 3030, 3183, 3366, 3399
GsaI CCCAGC 1 cut(s) 3405
GsuI CTGGAG 3 cut(s) 1931, 2754, 3024
HaeII RGCGCY 2 cut(s) 2258, 3238
HaeIII GGCC 6 cut(s) 1860, 2086, 2523, 3133, 3160, 3280
HapII CCGG 3 cut(s) 759, 1097, 1619
HgaI GACGC 2 cut(s) 398, 539
HhaI GCGC 3 cut(s) 2257, 2905, 3237
Hin6I GCGC 3 cut(s) 2255, 2903, 3235
HinP1I GCGC 3 cut(s) 2255, 2903, 3235
HincII GTYRAC 3 cut(s) 1257, 1825, 1949
HindII GTYRAC 3 cut(s) 1257, 1825, 1949
HindIII AAGCTT 7 cut(s) 957, 1055, 1103, 1309, 1499, 2498, 3117
HpaI GTTAAC 1 cut(s) 1825
HpaII CCGG 3 cut(s) 759, 1097, 1619
HphI GGTGA 9 cut(s) 404, 733, 1553, 2008, 2278, 2366, 2452, 3160, 3352
Hpy166II GTNNAC 8 cut(s) 429, 1094, 1216, 1257, 1825, 1904, 1949, 2818
Hpy8I GTNNAC 8 cut(s) 429, 1094, 1216, 1257, 1825, 1904, 1949, 2818
Hpy99I CGWCG 1 cut(s) 27
HpyCH4III ACNGT 9 cut(s) 270, 864, 1829, 2007, 2180, 2315, 2492, 2591, 3274
HpyCH4IV ACGT 3 cut(s) 25, 1507, 2834
HpySE526I ACGT 3 cut(s) 25, 1507, 2834
HspAI GCGC 3 cut(s) 2255, 2903, 3235
Kpn2I TCCGGA 1 cut(s) 1618
KpnI GGTACC 1 cut(s) 1547
KspAI GTTAAC 1 cut(s) 1825
Kzo9I GATC 7 cut(s) 277, 518, 2238, 2307, 2326, 2390, 2884
LmnI GCTCC 5 cut(s) 1351, 2798, 2845, 3070, 3356
Lsp1109I GCAGC 6 cut(s) 222, 2881, 3041, 3169, 3352, 3385
MabI ACCWGGT 1 cut(s) 2350
MaeI CTAG 6 cut(s) 398, 581, 609, 1247, 1607, 3156
MaeII ACGT 3 cut(s) 25, 1507, 2834
MaeIII GTNAC 7 cut(s) 163, 359, 410, 674, 711, 3103, 3340
MalI GATC 7 cut(s) 279, 520, 2240, 2309, 2328, 2392, 2886
MboI GATC 7 cut(s) 277, 518, 2238, 2307, 2326, 2390, 2884
MfeI CAATTG 3 cut(s) 246, 1478, 2409
MflI RGATCY 3 cut(s) 2307, 2390, 2884
MhlI GDGCHC 8 cut(s) 376, 741, 1876, 2599, 2795, 2842, 3114, 3155
MlsI TGGCCA 1 cut(s) 2086
MluNI TGGCCA 1 cut(s) 2086
MlyI GAGTC 4 cut(s) 8, 305, 2277, 2482
MmeI TCCRAC 3 cut(s) 1311, 2704, 3334
Mox20I TGGCCA 1 cut(s) 2086
MroI TCCGGA 1 cut(s) 1618
MroXI GAANNNNTTC 2 cut(s) 1959, 2969
MscI TGGCCA 1 cut(s) 2086
MslI CAYNNNNRTG 1 cut(s) 386
Msp20I TGGCCA 1 cut(s) 2086
MspA1I CMGCKG 1 cut(s) 1909
MspI CCGG 3 cut(s) 759, 1097, 1619
MspR9I CCNGG 6 cut(s) 57, 1870, 2352, 2668, 2937, 3282
MunI CAATTG 3 cut(s) 246, 1478, 2409
MvaI CCWGG 6 cut(s) 57, 1870, 2352, 2668, 2937, 3282
MvnI CGCG 1 cut(s) 22
NdeI CATATG 1 cut(s) 3095
NdeII GATC 7 cut(s) 277, 518, 2238, 2307, 2326, 2390, 2884
NlaIV GGNNCC 6 cut(s) 1545, 1931, 2841, 2886, 2952, 3113
NmeAIII GCCGAG 1 cut(s) 2094
NmuCI GTSAC 4 cut(s) 359, 410, 674, 3340
NruI TCGCGA 1 cut(s) 22
NspI RCATGY 3 cut(s) 712, 1362, 1977
NspV TTCGAA 2 cut(s) 1287, 2363
PaeR7I CTCGAG 1 cut(s) 1805
PagI TCATGA 1 cut(s) 417
PasI CCCWGGG 1 cut(s) 1869
PceI AGGCCT 1 cut(s) 3160
PciI ACATGT 2 cut(s) 708, 1358
PdmI GAANNNNTTC 2 cut(s) 1959, 2969
PfoI TCCNGGA 1 cut(s) 2935
PkrI GCNGC 6 cut(s) 237, 2896, 3031, 3184, 3367, 3400
PleI GAGTC 4 cut(s) 8, 305, 2277, 2481
PpsI GAGTC 4 cut(s) 8, 305, 2277, 2481
Ppu21I YACGTR 1 cut(s) 2835
PscI ACATGT 2 cut(s) 708, 1358
PshAI GACNNNNGTC 2 cut(s) 1224, 1944
PshBI ATTAAT 1 cut(s) 141
PsiI TTATAA 1 cut(s) 1170
Psp124BI GAGCTC 2 cut(s) 741, 2795
Psp6I CCWGG 6 cut(s) 55, 1868, 2350, 2666, 2935, 3280
PspFI CCCAGC 1 cut(s) 3401
PspGI CCWGG 6 cut(s) 55, 1868, 2350, 2666, 2935, 3280
PspN4I GGNNCC 6 cut(s) 1545, 1931, 2841, 2886, 2952, 3113
PspPI GGNCC 4 cut(s) 2521, 2951, 3131, 3278
PsrI GAACNNNNNNTAC 2 cut(s) 873, 905
PstI CTGCAG 1 cut(s) 3217
PstNI CAGNNNCTG 4 cut(s) 985, 1071, 2894, 3365
PsuI RGATCY 3 cut(s) 2307, 2390, 2884
PvuII CAGCTG 1 cut(s) 1909
RruI TCGCGA 1 cut(s) 22
RsaI GTAC 5 cut(s) 1545, 2826, 2833, 2912, 3271
RsaNI GTAC 5 cut(s) 1544, 2825, 2832, 2911, 3270
RseI CAYNNNNRTG 1 cut(s) 386
SacI GAGCTC 2 cut(s) 741, 2795
SatI GCNGC 6 cut(s) 236, 2895, 3030, 3183, 3366, 3399
Sau3AI GATC 7 cut(s) 277, 518, 2238, 2307, 2326, 2390, 2884
Sau96I GGNCC 4 cut(s) 2521, 2951, 3131, 3278
ScaI AGTACT 1 cut(s) 2826
SchI GAGTC 4 cut(s) 8, 305, 2277, 2482
ScrFI CCNGG 6 cut(s) 57, 1870, 2352, 2668, 2937, 3282
SduI GDGCHC 8 cut(s) 376, 741, 1876, 2599, 2795, 2842, 3114, 3155
SexAI ACCWGGT 1 cut(s) 2350
SfcI CTRYAG 2 cut(s) 2590, 3213
Sfr274I CTCGAG 1 cut(s) 1805
SfuI TTCGAA 2 cut(s) 1287, 2363
SinI GGWCC 1 cut(s) 2951
SlaI CTCGAG 1 cut(s) 1805
SmiMI CAYNNNNRTG 1 cut(s) 386
SmlI CTYRAG 4 cut(s) 971, 1805, 2268, 2468
SmoI CTYRAG 4 cut(s) 971, 1805, 2268, 2468
SseBI AGGCCT 1 cut(s) 3160
SsiI CCGC 5 cut(s) 112, 370, 2305, 2770, 2843
SspMI CTAG 6 cut(s) 398, 581, 609, 1247, 1607, 3156
SstI GAGCTC 2 cut(s) 741, 2795
StuI AGGCCT 1 cut(s) 3160
StyD4I CCNGG 6 cut(s) 55, 1868, 2350, 2666, 2935, 3280
StyI CCWWGG 3 cut(s) 748, 2796, 2946
TaaI ACNGT 9 cut(s) 270, 864, 1829, 2007, 2180, 2315, 2492, 2591, 3274
TaiI ACGT 3 cut(s) 28, 1510, 2837
TatI WGTACW 2 cut(s) 2824, 2910
TscAI CASTG 4 cut(s) 912, 2161, 2689, 3039
TseFI GTSAC 4 cut(s) 359, 410, 674, 3340
TseI GCWGC 6 cut(s) 235, 2894, 3029, 3182, 3365, 3398
Tsp45I GTSAC 4 cut(s) 359, 410, 674, 3340
TspGWI ACGGA 1 cut(s) 2860
TspRI CASTG 4 cut(s) 912, 2161, 2689, 3039
VpaK11BI GGWCC 1 cut(s) 2951
VspI ATTAAT 1 cut(s) 141
XagI CCTNNNNNAGG 1 cut(s) 1538
XapI RAATTY 7 cut(s) 403, 421, 767, 1959, 2359, 2805, 3124
XceI RCATGY 3 cut(s) 712, 1362, 1977
XhoI CTCGAG 1 cut(s) 1805
XmiI GTMKAC 2 cut(s) 428, 1903
XmnI GAANNNNTTC 2 cut(s) 1959, 2969
XspI CTAG 6 cut(s) 398, 581, 609, 1247, 1607, 3156
ZrmI AGTACT 1 cut(s) 2826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.