RchiOBHm_Chr1g0319741

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000070
Physical Location & Seq
Reverse (-)
7417560 .. 7428510
10951 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54996

Sequence Viewer

Length: 4095 bp
ATGGCAGTGGGAGAGATCTTTCTTGCGGCATTCCTTCAGTTGCTGCTGGACAGGTTAACGCCTCGGGAGATCCTGGGCTACTTTGGAAGCTTGGGAGGAGCTGGCAAAAAGCTGCAGAAATGGAGAGAGACACTTTCCGCAGTTGCAGCGGTGCTTTCCGATGCGGAGGAGAAGCAGCTGACGAGCAAGGCAGTGGATCTGTGGCTGAATGACCTGAAACACTTGGCTTATGATCTGGACGATCTACTCAACACCTTTTCTAATGAGATGTTAGAACGCGAGCAGCTCAAGCTTCACCAAACTGGCACAAGCAAGGTACGAGGCTTCTTTACCAAAGTTCCTCACAAAGTTAAGTTCAATTTCAATATGAACTCCGAAGTAGACGAGATTGCTAATCGGTTGCAAGACATATTTGAACGGAAAAAGAAACTCGGTTTGAAATATATTGAGCATACCTCTACTTCAACATCCTCATCGCACAGGACACCAAGTTCATATGTGTTGGATGGACCTGTGGTTGGGAGAGATGAAGACAGAAGAAAGATTGTGGAATTGTTGTCCAGAGATGCTGATCCTTCTTCTACCACCAAATATCAAGTTGTTGCCATTGTTGGTATGGGAGGACTCGGCAAGACAATGCTTGCGGGACAAGTCTTCAATGATGTAGCTGCAATGAAACAGTTTGATCTCAATATCTGGGTTTCAGTGTCTGATGACTTCGATCTTCAAACGGTGACGAGAACTATTTTTAAGGAAGTTACATCTCGGCCTTGTGATATGGATGAGTTCAGTCGACTTCAAGATAATCTGAGCAAGGAGATCGGTGGTAAAAGGTTTCTGATTGTTTTTGATGATGTCTGGAGCACGTGTGGTTATGATTCATGGACAAAACTGCAAGCTCCCCTCCGTGGCGGAGCCAAGGGAAGTAAGGTAATGCTGACAACACGTGATGAAAAAGTTGCGGCATTGATGGGAGCCCCAGCCACTGAAGTTTATTATTTAAAGACTCTATCAGATGAAGGTTGTTTGCAAGTATTTGAGCAGCATGTTAGCAATGACAGGCCACCGAATTTCGAGTTGCTTAAGAAGAAAATTGTTACGAACTGCAATGGATTGCCATTGGCTGCAAAAACTCTTGGGGGTGTTTTACGTTGTAAAGAAACTGACAAATGGGAGGAAATGCTGAATGACAAATTATGGAGTATATCAGATGAGAGTAACATACTCCCAGTACTCAGATTGAGTTATCATTATCTCCCTTCACCTTTGAAGAGGTGCTTTGCCTATTGCTCAATACTTCCCAATGACTATGAATTTGGGAAAACCCAATTAATCCTTCTGTGGATGGCAGAGGGTTTCCTTGAGCAAACAGAAGGGACTAAAGGAATGGAAGATATCGGTGACGAATATTTTGGGGAGCTATTGTCTCGGTCATTATTTCAAACCTCGGGCAAAAACAGTTCATGCTATGTAATGCATGACCTTGTTGGTGATTTGGCACGATGGGCTGCTGCAGACACATTTTGCAGATTGGAGGATAAACAGCATGGTAGATGTTCTGCAAAGACTCGTCATCTGAGTTACATTTCTGCTAAGTTTGATGGGGAAAAAAGATTTGAGACAATTTCTGGAGCAAAACGCTTGCGGACTTTCCTACCACTTCCTGTTTCCGGTGGTTATGAGAATTATCTAACTCGTTATGCTACTTTCGATATATTGCCACAATTGAAGTACTTGCGGGTGCTCTCCTTCAATGGCTATAAACTGACCGAGGTGCCAAATTCCGTAGGTAAGTTGAGACATCTACGGTACCTTGATCTTTCTCACACATTAATAACGTGTTTGCCTGAGTCAACATGCATGCTTTACAACTTACAGACGTTAATATTAGAGAATTGTTCAAAATTGAAGACACTACCTTCAAACATGAGCAATTTATCTAATTTACGTCATCTCAACAATTCAAACATGCCTTCATTGGAAGAAATGCCTCCCAAAGTAAGTCGGTTGACTCATCTGCGAACTTTGCCTAATTTTGTGGTGGGGAAAGGTAGTGCATCAGGGATTGGAGAGATACAGTCATTGCATCTTCGTGGGACCTTGAGTGTTGGAAGATTGGAAAATGTGATTGATGTTGGGGATGCGAAGACGGCTCAAATAATGAACAAGGAAGGGCTGGAAATCTTGCAATTGGAATGGAGCGGCACAAGCGAGAAGGAGTTGGAGGTGCTGTGCAGTTTAGAACCTCATAAAAAGCTTAAGGAGCTGACCATCAAGGGCTACAATGGTTTCGAATTTTCAAAATGGATTGGACATCCTTCATTCTCTGATATGACATCTGTGAAATTGGAGAATTGTAAAAACTGTCGATTCTTACCTCCTCTTGGCCAATTACCTTTTCTGAAAAATCTTCAGATACTAGGACTGGTCAATGTTGAAAGTGTGGGTGCTGAGTTTTATGGAGAGTGCAGCTTGCCTTTTCCAGTGTTGGAATGCATGACGTTCCATGACATGCAAAATTGGAAGGAGTGGCTTCCTTGCAAAAGAGATGAAGAAATTCGAGTTTTCCCATGCATGGAAAAGCTTTCCATCCTTGGTTGCCCCAAATTGAAAGGTTGGTTGCCCAAGAACATGGATTCATTATCACAGCTGTTTATTGCTGAATGTGAAGAGTTGGTGGTTTCAATAGCCAATTATAAACATCTTCGTATGCTGATCATCGACGGTTGTAAAAGGGTGGTGCACAGAAGTGGAGTTAAGTTTGAGTTACTGGAGGCCATGGGGCTTTATAATATTTCAGAGTTCAGACTCGAAATAGACGGGTTCATCAGAGGATTGCCAAAGCTTCAAACTTTGCGGATTACTGGTTGTGAAGAGTTGACATGTTTATGGGAGAATGAGGATAGATGGCTGCAGCGTCGAGTTTCTAATATTATTGGAGGCAACATATCTGACTCCCCCTCTCACTTTATTCAAGAGCTAAGAGCACTCAAGGAACTTGTGCTAACTGGATGCTCAAATCTAATTTCTTTTCCAGAAGCTGGTTGGCCACCTTGTCTTGAATGTGTAAAGATGGAGTCGTGTAATTCTTTGACATATTTCGTAAGGTATCAAGTTCCGCCAAGCATAAGAATGATAGAGATAGAAAAATGTCAAAATTTGAAACTATTAGTGAAGGATGCTGGGGAGCTAGAGGGTTGTCTGGAGTACTTGGATATAAAAGAGTGTGCATCTTTGACATCCTTATCAGGCGAAGGAGGCCGACTACCCAGAACGCTCAAATATCTAGAGATACGTGACTGTGAACAATTGGAGTCAATAATCAAAACTTTCCACGATGACACTTGTCTTGAAGGTCTTGGGATAGAGAGGTGTGCAAATCTCAAATCCTTACCAGAGGGGCTATGCCATCTCTCCGCTCTTTGCATTTTAGTTATAGCTGAATGTGGAAGTCTCGTTTCCTTCCCAAGAGGAGGGTTGCCATCCAACCTGATATCGTTTCGTATCGACGATTGTGATCAATTGGAAGCCGTCCCCAGAGGCATGGACAACCTCAGCTCTCTTCAGACTTTGTCGTTGGCATACCGTGGAGGTTTGGCATCCATTCTAGAAGAGGGTCTCCCACCCAACCTAATTACTCTTAAAATTGGCGATGCCGAAAGTCTCAAGCCTCTCTCAGAGTGGGGGCTTCACCACTTGGACAGACTCACCTCTCTTAAAGACTTGCTTGTCATTGGTTTCGATCCAGATCTGGTGTCATTTCCGCCAGAGGAGGTGCTGCTCCCTAAATCTCTCATTAAACTCACCATTGCAGGCTTCCCAAATCTGAAGCGCCTGTCATCATCTTTTCAATCACTCACCTCTCTTGAATCCCTTAACATTATGCATTGCCCAAAGCTAGCATCAATTATTCTAGAAAAAGAGGATTATCTGCCTCCTTCACTTACACGGCTTCGCATCTTTGAGGTATGTCCGCTGCTCACAAAGATATATCAACCAGGTAAAGGACGACACTGGCCCAAACAAATAGCCCACATCCCTTACGTTTTTGTTGGAGACTCCACTGAGGAAGCCGAAGCCGACCGCTGGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1364

Amino Acids

153.63

Weight (kDa)

6.09

Isoelectric Point (pI)

46.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 99 4.8e-17 Rx N-terminal domain
NB-ARC PF00931 180 - 352 4.7e-36 NB-ARC domain
WHD_DRP PF23559 432 - 500 3.5e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 573 - 839 3.1e-22 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 689 - 807 3e-33 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2697, 2790
AasI GACNNNNNNGTC 1 cut(s) 3728
Acc65I GGTACC 1 cut(s) 1809
AccB1I GGYRCC 2 cut(s) 1774, 1809
AccB7I CCANNNNNTGG 1 cut(s) 3041
AccBSI CCGCTC 2 cut(s) 2202, 3419
AccI GTMKAC 2 cut(s) 381, 793
AccII CGCG 1 cut(s) 279
AclWI GGATC 4 cut(s) 64, 204, 566, 3737
AcoI YGGCCR 2 cut(s) 2386, 3047
AcsI RAATTY 6 cut(s) 1069, 1313, 1780, 2294, 2556, 3157
AcuI CTGAAG 5 cut(s) 20, 1008, 2396, 3548, 3848
AcvI CACGTG 2 cut(s) 867, 947
AfaI GTAC 5 cut(s) 318, 1233, 1733, 1811, 3209
AfiI CCNNNNNNNGG 9 cut(s) 518, 908, 1670, 2384, 2575, 3041, 3473, 4004, 4086
AflII CTTAAG 2 cut(s) 1082, 2258
AflIII ACRYGT 4 cut(s) 866, 944, 1838, 2882
AjnI CCWGG 2 cut(s) 72, 3997
AjuI GAANNNNNNNTTGG 4 cut(s) 1320, 1352, 2382, 2414
AleI CACNNNNGTG 1 cut(s) 2748
AloI GAACNNNNNNTCC 2 cut(s) 475, 507
Alw21I GWGCWC 4 cut(s) 866, 1746, 2745, 2989
Alw26I GTCTC 8 cut(s) 122, 1431, 1613, 1792, 3458, 3623, 3668, 4050
Alw44I GTGCAC 1 cut(s) 2741
AlwI GGATC 4 cut(s) 64, 204, 566, 3737
AlwNI CAGNNNCTG 4 cut(s) 43, 710, 986, 3041
Ama87I CYCGRG 2 cut(s) 63, 1447
AoxI GGCC 7 cut(s) 767, 1061, 2386, 2775, 3047, 3259, 4016
ApaLI GTGCAC 1 cut(s) 2741
ApoI RAATTY 6 cut(s) 1069, 1313, 1780, 2294, 2556, 3157
ArsI GACNNNNNNTTYG 4 cut(s) 1298, 1330, 1394, 1426
AseI ATTAAT 2 cut(s) 1331, 1832
Asp700I GAANNNNTTC 1 cut(s) 2556
Asp718I GGTACC 1 cut(s) 1809
AspLEI GCGC 1 cut(s) 3834
AspS9I GGNCC 3 cut(s) 509, 2097, 4017
AsuHPI GGTGA 9 cut(s) 287, 745, 1254, 1412, 1502, 3683, 3700, 3796, 3850
AsuII TTCGAA 1 cut(s) 2292
AsuNHI GCTAGC 1 cut(s) 3898
AvaI CYCGRG 2 cut(s) 63, 1447
AvaII GGWCC 2 cut(s) 509, 2097
BaeGI GKGCMC 1 cut(s) 2745
BalI TGGCCA 2 cut(s) 2388, 3049
BanI GGYRCC 2 cut(s) 1774, 1809
BanII GRGCYC 1 cut(s) 979
BbrPI CACGTG 2 cut(s) 867, 947
BbsI GAAGAC 4 cut(s) 537, 646, 1916, 2153
Bbv12I GWGCWC 4 cut(s) 866, 1746, 2745, 2989
BbvCI CCTCAGC 1 cut(s) 3554
BceAI ACGGC 3 cut(s) 2166, 3515, 3965
BcgI CGANNNNNNTGC 2 cut(s) 802, 836
BciT130I CCWGG 2 cut(s) 74, 3999
BclI TGATCA 2 cut(s) 2715, 3517
BcoDI GTCTC 8 cut(s) 122, 1431, 1613, 1792, 3458, 3623, 3668, 4050
BfaI CTAG 6 cut(s) 2420, 3191, 3287, 3608, 3899, 3914
BfmI CTRYAG 3 cut(s) 113, 1512, 2912
BfoI RGCGCY 1 cut(s) 3835
BfrI CTTAAG 2 cut(s) 1082, 2258
BglII AGATCT 2 cut(s) 15, 3748
BmcAI AGTACT 3 cut(s) 1233, 1733, 3209
Bme1390I CCNGG 2 cut(s) 74, 3999
Bme18I GGWCC 2 cut(s) 509, 2097
BmeT110I CYCGRG 2 cut(s) 63, 1447
BmgT120I GGNCC 3 cut(s) 509, 2097, 4017
BmiI GGNNCC 5 cut(s) 916, 976, 1776, 1811, 2098
BmrFI CCNGG 2 cut(s) 74, 3999
BmrI ACTGGG 1 cut(s) 1223
BmtI GCTAGC 1 cut(s) 3902
BmuI ACTGGG 1 cut(s) 1223
BoxI GACNNNNGTC 1 cut(s) 3345
BpiI GAAGAC 4 cut(s) 537, 646, 1916, 2153
BplI GAGNNNNNCTC 2 cut(s) 440, 472
BpmI CTGGAG 4 cut(s) 880, 1650, 2792, 3224
Bpu10I CCTNAGC 1 cut(s) 3554
Bpu14I TTCGAA 1 cut(s) 2292
BpuEI CTTGAG 5 cut(s) 272, 1382, 2122, 2975, 3650
BsaAI YACGTR 3 cut(s) 867, 947, 3296
BsaBI GATNNNNATC 3 cut(s) 570, 3516, 3747
BsaI GGTCTC 1 cut(s) 3623
BsaJI CCNNGG 9 cut(s) 62, 73, 907, 918, 1446, 1770, 2593, 2778, 3586
BsaWI WCCGGW 1 cut(s) 1670
BsaXI ACNNNNNCTCC 6 cut(s) 853, 883, 1409, 1439, 2216, 2246
Bsc4I CCNNNNNNNGG 9 cut(s) 518, 908, 1670, 2384, 2575, 3041, 3473, 4004, 4086
Bse1I ACTGG 8 cut(s) 307, 1229, 2430, 2483, 2775, 2869, 3013, 4019
Bse3DI GCAATG 6 cut(s) 678, 1060, 1114, 2081, 3807, 3886
Bse8I GATNNNNATC 3 cut(s) 570, 3516, 3747
BseBI CCWGG 2 cut(s) 74, 3999
BseDI CCNNGG 9 cut(s) 62, 73, 907, 918, 1446, 1770, 2593, 2778, 3586
BseJI GATNNNNATC 3 cut(s) 570, 3516, 3747
BseLI CCNNNNNNNGG 9 cut(s) 518, 908, 1670, 2384, 2575, 3041, 3473, 4004, 4086
BseMI GCAATG 6 cut(s) 678, 1060, 1114, 2081, 3807, 3886
BseMII CTCAG 8 cut(s) 800, 1249, 1568, 1839, 2442, 3568, 3690, 4056
BseNI ACTGG 8 cut(s) 307, 1229, 2430, 2483, 2775, 2869, 3013, 4019
BseRI GAGGAG 5 cut(s) 111, 182, 2370, 3486, 3785
BseSI GKGCMC 1 cut(s) 2745
BseYI CCCAGC 3 cut(s) 979, 3182, 4086
BsgI GTGCAG 2 cut(s) 2254, 2488
Bsh1236I CGCG 1 cut(s) 279
Bsh1285I CGRYCG 1 cut(s) 4084
BshFI GGCC 7 cut(s) 769, 1063, 2388, 2777, 3049, 3261, 4018
BshNI GGYRCC 2 cut(s) 1774, 1809
BsiEI CGRYCG 1 cut(s) 4084
BsiHKAI GWGCWC 4 cut(s) 866, 1746, 2745, 2989
BsiHKCI CYCGRG 2 cut(s) 63, 1447
BsiSI CCGG 1 cut(s) 1671
BslFI GGGAC 4 cut(s) 660, 1390, 2110, 3518
BslI CCNNNNNNNGG 9 cut(s) 518, 908, 1670, 2384, 2575, 3041, 3473, 4004, 4086
BsmAI GTCTC 8 cut(s) 122, 1431, 1613, 1792, 3458, 3623, 3668, 4050
BsmFI GGGAC 4 cut(s) 660, 1390, 2110, 3518
BsmI GAATGC 2 cut(s) 29, 2498
BsnI GGCC 7 cut(s) 769, 1063, 2388, 2777, 3049, 3261, 4018
Bso31I GGTCTC 1 cut(s) 3623
BsoBI CYCGRG 2 cut(s) 63, 1447
Bsp119I TTCGAA 1 cut(s) 2292
Bsp1286I GDGCHC 5 cut(s) 866, 979, 1746, 2745, 2989
Bsp19I CCATGG 1 cut(s) 2778
BspANI GGCC 7 cut(s) 769, 1063, 2388, 2777, 3049, 3261, 4018
BspCNI CTCAG 8 cut(s) 801, 1248, 1569, 1840, 2443, 3567, 3689, 4057
BspFNI CGCG 1 cut(s) 279
BspLI GGNNCC 5 cut(s) 916, 976, 1776, 1811, 2098
BspMAI CTGCAG 3 cut(s) 117, 1516, 2916
BspOI GCTAGC 1 cut(s) 3902
BspPI GGATC 4 cut(s) 64, 204, 566, 3737
BspT104I TTCGAA 1 cut(s) 2292
BspT107I GGYRCC 2 cut(s) 1774, 1809
BspTI CTTAAG 2 cut(s) 1082, 2258
BspTNI GGTCTC 1 cut(s) 3623
BsrBI CCGCTC 2 cut(s) 2202, 3419
BsrDI GCAATG 6 cut(s) 678, 1060, 1114, 2081, 3807, 3886
BsrI ACTGG 8 cut(s) 307, 1229, 2430, 2483, 2775, 2869, 3013, 4019
BssECI CCNNGG 9 cut(s) 62, 73, 907, 918, 1446, 1770, 2593, 2778, 3586
BssT1I CCWWGG 3 cut(s) 918, 2593, 2778
Bst2UI CCWGG 2 cut(s) 74, 3999
Bst4CI ACNGT 9 cut(s) 681, 733, 1460, 1809, 2079, 2366, 2726, 3302, 3587
Bst6I CTCTTC 5 cut(s) 1265, 2664, 2868, 3567, 3606
BstAFI CTTAAG 2 cut(s) 1082, 2258
BstBAI YACGTR 3 cut(s) 867, 947, 3296
BstBI TTCGAA 1 cut(s) 2292
BstC8I GCNNGC 9 cut(s) 103, 281, 642, 897, 1643, 1862, 2474, 3814, 3900
BstDSI CCRYGG 3 cut(s) 907, 2778, 3586
BstFNI CGCG 1 cut(s) 279
BstH2I RGCGCY 1 cut(s) 3835
BstHHI GCGC 1 cut(s) 3834
BstMAI GTCTC 8 cut(s) 122, 1431, 1613, 1792, 3458, 3623, 3668, 4050
BstMCI CGRYCG 1 cut(s) 4084
BstMWI GCNNNNNNNGC 8 cut(s) 146, 289, 1505, 2026, 2150, 2208, 2263, 3258
BstNI CCWGG 2 cut(s) 74, 3999
BstNSI RCATGY 6 cut(s) 1049, 1860, 1864, 1972, 2515, 2886
BstPAI GACNNNNGTC 1 cut(s) 3345
BstSCI CCNGG 2 cut(s) 72, 3997
BstSFI CTRYAG 3 cut(s) 113, 1512, 2912
BstSLI GKGCMC 1 cut(s) 2745
BstUI CGCG 1 cut(s) 279
BstV2I GAAGAC 4 cut(s) 537, 646, 1916, 2153
BstX2I RGATCY 4 cut(s) 15, 69, 196, 3748
BstXI CCANNNNNNTGG 2 cut(s) 2632, 3544
BstYI RGATCY 4 cut(s) 15, 69, 196, 3748
BsuRI GGCC 7 cut(s) 769, 1063, 2388, 2777, 3049, 3261, 4018
BtgI CCRYGG 3 cut(s) 907, 2778, 3586
BtgZI GCGATG 2 cut(s) 459, 3666
BtsI GCAGTG 2 cut(s) 12, 198
BtsIMutI CAGTG 7 cut(s) 12, 198, 711, 984, 2490, 4012, 4062
Cac8I GCNNGC 9 cut(s) 103, 281, 642, 897, 1643, 1862, 2474, 3814, 3900
CaiI CAGNNNCTG 4 cut(s) 43, 710, 986, 3041
CfoI GCGC 1 cut(s) 3834
Cfr13I GGNCC 3 cut(s) 509, 2097, 4017
CseI GACGC 1 cut(s) 2906
CsiI ACCWGGT 1 cut(s) 3997
Csp6I GTAC 5 cut(s) 317, 1232, 1732, 1810, 3208
CviQI GTAC 5 cut(s) 317, 1232, 1732, 1810, 3208
DraI TTTAAA 1 cut(s) 1002
DrdI GACNNNNNNGTC 1 cut(s) 3728
DseDI GACNNNNNNGTC 1 cut(s) 3728
EaeI YGGCCR 2 cut(s) 2386, 3047
Eam1104I CTCTTC 5 cut(s) 1265, 2664, 2868, 3567, 3606
EarI CTCTTC 5 cut(s) 1265, 2664, 2868, 3567, 3606
EciI GGCGGA 3 cut(s) 927, 3108, 3753
Eco130I CCWWGG 3 cut(s) 918, 2593, 2778
Eco24I GRGCYC 1 cut(s) 979
Eco31I GGTCTC 1 cut(s) 3623
Eco32I GATATC 2 cut(s) 1396, 3495
Eco47I GGWCC 2 cut(s) 509, 2097
Eco57I CTGAAG 5 cut(s) 20, 1008, 2396, 3548, 3848
Eco72I CACGTG 2 cut(s) 867, 947
Eco88I CYCGRG 2 cut(s) 63, 1447
EcoO109I RGGNCCY 1 cut(s) 2097
EcoRII CCWGG 2 cut(s) 72, 3997
EcoRV GATATC 2 cut(s) 1396, 3495
EcoT14I CCWWGG 3 cut(s) 918, 2593, 2778
EcoT22I ATGCAT 5 cut(s) 1479, 1862, 2498, 2576, 3888
EcoT38I GRGCYC 1 cut(s) 979
ErhI CCWWGG 3 cut(s) 918, 2593, 2778
FalI AAGNNNNNCTT 4 cut(s) 1262, 1294, 3711, 3743
FaqI GGGAC 4 cut(s) 660, 1390, 2110, 3518
FauI CCCGC 2 cut(s) 637, 1731
FauNDI CATATG 1 cut(s) 496
FbaI TGATCA 2 cut(s) 2715, 3517
FblI GTMKAC 2 cut(s) 381, 793
FriOI GRGCYC 1 cut(s) 979
FspBI CTAG 6 cut(s) 2420, 3191, 3287, 3608, 3899, 3914
GlaI GCGC 1 cut(s) 3833
GsaI CCCAGC 3 cut(s) 983, 3186, 4090
GsuI CTGGAG 4 cut(s) 880, 1650, 2792, 3224
HaeII RGCGCY 1 cut(s) 3835
HaeIII GGCC 7 cut(s) 769, 1063, 2388, 2777, 3049, 3261, 4018
HapII CCGG 1 cut(s) 1671
HgaI GACGC 1 cut(s) 2906
HhaI GCGC 1 cut(s) 3834
Hin6I GCGC 1 cut(s) 3832
HinP1I GCGC 1 cut(s) 3832
HincII GTYRAC 5 cut(s) 57, 794, 1854, 2010, 2880
HindII GTYRAC 5 cut(s) 57, 794, 1854, 2010, 2880
HindIII AAGCTT 5 cut(s) 88, 290, 2255, 2582, 2843
HpaI GTTAAC 1 cut(s) 57
HpaII CCGG 1 cut(s) 1671
HphI GGTGA 9 cut(s) 287, 745, 1254, 1412, 1502, 3683, 3700, 3796, 3850
Hpy166II GTNNAC 8 cut(s) 57, 382, 794, 1854, 2010, 2743, 2880, 3305
Hpy8I GTNNAC 8 cut(s) 57, 382, 794, 1854, 2010, 2743, 2880, 3305
Hpy99I CGWCG 3 cut(s) 2726, 2922, 3512
HpyCH4III ACNGT 9 cut(s) 681, 733, 1460, 1809, 2079, 2366, 2726, 3302, 3587
HpyCH4IV ACGT 9 cut(s) 866, 946, 1150, 1838, 1880, 1948, 2501, 3295, 4044
HpyF10VI GCNNNNNNNGC 8 cut(s) 146, 289, 1505, 2026, 2150, 2208, 2263, 3258
HpySE526I ACGT 9 cut(s) 866, 946, 1150, 1838, 1880, 1948, 2501, 3295, 4044
HspAI GCGC 1 cut(s) 3832
KpnI GGTACC 1 cut(s) 1813
Ksp22I TGATCA 2 cut(s) 2715, 3517
KspAI GTTAAC 1 cut(s) 57
MabI ACCWGGT 1 cut(s) 3997
MaeI CTAG 6 cut(s) 2420, 3191, 3287, 3608, 3899, 3914
MaeII ACGT 9 cut(s) 866, 946, 1150, 1838, 1880, 1948, 2501, 3295, 4044
MaeIII GTNAC 8 cut(s) 733, 757, 1096, 1217, 1400, 1580, 2766, 3296
MbiI CCGCTC 2 cut(s) 2202, 3419
MfeI CAATTG 4 cut(s) 1724, 2189, 3308, 3521
MflI RGATCY 4 cut(s) 15, 69, 196, 3748
MhlI GDGCHC 5 cut(s) 866, 979, 1746, 2745, 2989
MlsI TGGCCA 2 cut(s) 2388, 3049
MluNI TGGCCA 2 cut(s) 2388, 3049
MmeI TCCRAC 6 cut(s) 483, 2089, 2202, 2469, 3510, 4033
Mox20I TGGCCA 2 cut(s) 2388, 3049
Mph1103I ATGCAT 5 cut(s) 1479, 1862, 2498, 2576, 3888
MroXI GAANNNNTTC 1 cut(s) 2556
MscI TGGCCA 2 cut(s) 2388, 3049
MslI CAYNNNNRTG 4 cut(s) 2091, 2748, 2887, 3131
Msp20I TGGCCA 2 cut(s) 2388, 3049
MspA1I CMGCKG 5 cut(s) 149, 178, 2650, 3976, 4086
MspCI CTTAAG 2 cut(s) 1082, 2258
MspI CCGG 1 cut(s) 1671
MspR9I CCNGG 2 cut(s) 74, 3999
MunI CAATTG 4 cut(s) 1724, 2189, 3308, 3521
Mva1269I GAATGC 2 cut(s) 29, 2498
MvaI CCWGG 2 cut(s) 74, 3999
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 8 cut(s) 146, 289, 1505, 2026, 2150, 2208, 2263, 3258
NcoI CCATGG 1 cut(s) 2778
NdeI CATATG 1 cut(s) 496
NheI GCTAGC 1 cut(s) 3898
NlaIV GGNNCC 5 cut(s) 916, 976, 1776, 1811, 2098
NmeAIII GCCGAG 2 cut(s) 606, 745
NmuCI GTSAC 3 cut(s) 733, 1400, 3296
NsiI ATGCAT 5 cut(s) 1479, 1862, 2498, 2576, 3888
NspI RCATGY 6 cut(s) 1049, 1860, 1864, 1972, 2515, 2886
NspV TTCGAA 1 cut(s) 2292
OliI CACNNNNGTG 1 cut(s) 2748
PaeI GCATGC 1 cut(s) 1864
PciI ACATGT 1 cut(s) 2882
PctI GAATGC 2 cut(s) 29, 2498
PdmI GAANNNNTTC 1 cut(s) 2556
PfeI GAWTC 4 cut(s) 878, 2370, 2636, 3869
PflFI GACNNNGTC 1 cut(s) 3571
PflMI CCANNNNNTGG 1 cut(s) 3041
PmaCI CACGTG 2 cut(s) 867, 947
PmlI CACGTG 2 cut(s) 867, 947
Ppu21I YACGTR 3 cut(s) 867, 947, 3296
PpuMI RGGWCCY 1 cut(s) 2097
PscI ACATGT 1 cut(s) 2882
PshAI GACNNNNGTC 1 cut(s) 3345
PshBI ATTAAT 2 cut(s) 1331, 1832
PsiI TTATAA 2 cut(s) 2697, 2790
Psp5II RGGWCCY 1 cut(s) 2097
Psp6I CCWGG 2 cut(s) 72, 3997
PspCI CACGTG 2 cut(s) 867, 947
PspFI CCCAGC 3 cut(s) 979, 3182, 4086
PspGI CCWGG 2 cut(s) 72, 3997
PspN4I GGNNCC 5 cut(s) 916, 976, 1776, 1811, 2098
PspPI GGNCC 3 cut(s) 509, 2097, 4017
PspPPI RGGWCCY 1 cut(s) 2097
PstI CTGCAG 3 cut(s) 117, 1516, 2916
PstNI CAGNNNCTG 4 cut(s) 43, 710, 986, 3041
PsuI RGATCY 4 cut(s) 15, 69, 196, 3748
PsyI GACNNNGTC 1 cut(s) 3571
PvuII CAGCTG 2 cut(s) 178, 2650
RsaI GTAC 5 cut(s) 318, 1233, 1733, 1811, 3209
RsaNI GTAC 5 cut(s) 317, 1232, 1732, 1810, 3208
RseI CAYNNNNRTG 4 cut(s) 2091, 2748, 2887, 3131
SalI GTCGAC 1 cut(s) 792
Sau96I GGNCC 3 cut(s) 509, 2097, 4017
ScaI AGTACT 3 cut(s) 1233, 1733, 3209
ScrFI CCNGG 2 cut(s) 74, 3999
SduI GDGCHC 5 cut(s) 866, 979, 1746, 2745, 2989
SexAI ACCWGGT 1 cut(s) 3997
SfcI CTRYAG 3 cut(s) 113, 1512, 2912
SfuI TTCGAA 1 cut(s) 2292
SinI GGWCC 2 cut(s) 509, 2097
SmiMI CAYNNNNRTG 4 cut(s) 2091, 2748, 2887, 3131
SmlI CTYRAG 7 cut(s) 287, 1082, 1361, 2101, 2258, 2990, 3665
SmoI CTYRAG 7 cut(s) 287, 1082, 1361, 2101, 2258, 2990, 3665
SphI GCATGC 1 cut(s) 1864
SspI AATATT 4 cut(s) 1409, 1887, 2794, 2932
SspMI CTAG 6 cut(s) 2420, 3191, 3287, 3608, 3899, 3914
StyD4I CCNGG 2 cut(s) 72, 3997
StyI CCWWGG 3 cut(s) 918, 2593, 2778
TaaI ACNGT 9 cut(s) 681, 733, 1460, 1809, 2079, 2366, 2726, 3302, 3587
TaiI ACGT 9 cut(s) 869, 949, 1153, 1841, 1883, 1951, 2504, 3298, 4047
TaqII GACCGA 2 cut(s) 1419, 1784
TatI WGTACW 3 cut(s) 1231, 1731, 3207
TauI GCSGC 3 cut(s) 29, 965, 2205
TfiI GAWTC 4 cut(s) 878, 2370, 2636, 3869
TscAI CASTG 7 cut(s) 12, 198, 711, 991, 2490, 4019, 4069
TseFI GTSAC 3 cut(s) 733, 1400, 3296
Tsp45I GTSAC 3 cut(s) 733, 1400, 3296
TspGWI ACGGA 3 cut(s) 433, 896, 1774
TspRI CASTG 7 cut(s) 12, 198, 711, 991, 2490, 4019, 4069
Tth111I GACNNNGTC 1 cut(s) 3571
Van91I CCANNNNNTGG 1 cut(s) 3041
Vha464I CTTAAG 2 cut(s) 1082, 2258
VneI GTGCAC 1 cut(s) 2741
VpaK11BI GGWCC 2 cut(s) 509, 2097
VspI ATTAAT 2 cut(s) 1331, 1832
XapI RAATTY 6 cut(s) 1069, 1313, 1780, 2294, 2556, 3157
XbaI TCTAGA 3 cut(s) 3286, 3607, 3913
XceI RCATGY 6 cut(s) 1049, 1860, 1864, 1972, 2515, 2886
XcmI CCANNNNNNNNNTGG 2 cut(s) 613, 3042
XmiI GTMKAC 2 cut(s) 381, 793
XmnI GAANNNNTTC 1 cut(s) 2556
XspI CTAG 6 cut(s) 2420, 3191, 3287, 3608, 3899, 3914
ZrmI AGTACT 3 cut(s) 1233, 1733, 3209
Zsp2I ATGCAT 5 cut(s) 1479, 1862, 2498, 2576, 3888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.