Prupe.2G028000_v2.0.a1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000070
Physical Location & Seq
Reverse (-)
2901866 .. 2908104
6239 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G028000.1

Sequence Viewer

Length: 4050 bp
ATGGCACTTGGAGAGATCTTTCTTGCTGCATTCCTTCAACTGCTGCTCGACCGGTTGACTCCTCGCAACTTTCTCAACTTTGCACAGAAAGAGGGTGTTGGCAAGAAGATGAAGAAATGGACTACAATGTTATCTGCAATTGGAGCGGTGCTGCATGATGCGGAGGAAAAGCAACTAATGAGCGAGGCTGTGAAACTGTGGCTGGATGATCTCAAAGATTTGGCTTATGATGTGGAAGACATGTTAGACAAGTTTTCCACTGAGATGTTTCGGCGCAAGGCACATAAGCTACATGGGGCTACCACAAGTAAGGTACGAAGCTTAATTCCAAGAGTTAAATTTAACAGTAGTATGAACTCAGAAATAAAGGTGATTACTGATCGATTACATGAGATATCTGAACGAAAAGACAAGCTAGGTTTAAATTATATTGGCACAACTTCTAGTAAGGCACGCCAGAGGTCACCAAGTTCAAGTGTGTTAGATGGACCAGTTGTTGGAAGAGATGGTGATAAAGCGAAGATTCTGGAATTGCTGTCAAGAGATGAGCCTAGTTCTGCCAATTTTCATGTAGTTTCTATTGTTGGCATGGCTGGAGTTGGGAAGACAACGCTTGCTCAGCTTGTATTCAACGACAACGATGTTTCCATGAAATTCAGTCCTAAGGCATGGGTGTCCGTGTCTGATGATTTTAACATTGTTAGAGTAACAAGGGCAATTCTTGAATCAATCACGTCCCGGCACTGTGATTTGGAGGAATTCAGCAACATTCAGGATAATCTGAGCAAGGAATTAGCAGGTAAAAAGTTTTTAATTGTTTTAGATGATGTTTGGAATACATGTGACTATGATCTATGGATCAAACTTCAGTCGCCTTTTCGTGTTGGAGCATTAGGAAGTAAGGTAATTGTGACAACACGTGATGGAGAAGTTGCAAAAATGATGAGAGCCATTGAAGTTCATAATTTGGAGTGCATATCAAATGACGATTGTTGGAGAGTGTTTGAGCAGCATGCATTCTTGAATGTACAGCCACCAAATATTGAGTTATATAGGGAGAAAATTGCTATAAAATGTGGTGGATTGCCTTTGGCCGCAAGGACTCTTGGTGGCCTTTTGGGTTGTAAAGAAATAGATGAATGGGAAGAAATACTGAATAGCAATTTGTGGAAGCTATCAGATAAAATAAACATTCTGCCAGTATTAAACGTGAGCTACCATTATCTCGCATCAAGTTTGAAAAGGTGTTTTGCCTATTGCTCAATACTTCCAAATGACTATGAGTTTGGAGAGAAGCAGTTGATTCTTCTCTGGATGGCAGAGGGTTTGATTCAGCAATCTGAAGAAAATAAACAAATGGAAGATATAGGCGGTGAGTATTTTCGTGAGCTAATATCTAGGTCATTATTTCAAAAGTCAAGTAAAAACAATTCACAATATGTAATGCATGACCTCGTTAGTGAATTGGCACGGTGGGCTGCAGGAGAAACATGTTTTAGATTGGATGATAGCATGCAACGCAGATTTTCTCCAAAGGTTCGTCATATGTCATACATCTCCGGTGAGTTTGATGGAGTTAAAAAATTGAAGGCCTTTTTTGAGGCCACTCATCTGCGAACTTTCCTACCACTTCAACTTTCAGATGCTCGGAGGAATAGCCTGACTAGCAAGGTTAATCATGATTTATTGCCAAAATTGCAATACTTGAGAGTGCTCTCTTTGAATGGCTATACAATAACTGAGCTCTCAAATTCAATTGGTGAATTGAAGTTTCTACGATACCTTGATCTTTCTCACACTCTAATACTGAGTTTGCCTGAATCATTAAGCACTCTTTACAACCTACAGACGTTGATACTAGAAAATTGTTCTCGTTTGAAGGCGCTACCGACAAACTTGAGTAATCTAATTAATCTGCGGCATCTCAACATTTCTGATGTACCCTTGTTAGAAGGCATGCCACCGCAATTAGGTCAATTGGCTAATCTGCAAACCTTGACTAATTTTGTTGTGGGCGAAAGTCATGAGTCAAAAATCAGAGAGATAGGGCCACTTCGGCATCTCAAATGGACATTGCACCTCTCAGGATTGGAGAATGTGATTGATGCTGAGGATGCAAGGAGGGCTGACTTAATTAGCAAGGACGGGCTTGATGTTTTGGCACTTAAATGGAAATACATGCGAGAACCGAAATCAGATGTGCTTGACATGTTACGACCTTCTAGAAAGCTCAAACAACTTACGATCATAGGTTATGGCGGTTTAGAATTTGCAACATGGCTGGGAGATCCTTTATTCTCTAATATGGTGCTCATAAGATTATACAATTGTAATAATTGTCAGTTCTTGCCACCACTTGGGAAATTGCCTTGTCTCAAAGAACTTCATATAATAGGAATGCCTGGAGTGGAAAGCGTGGGTCTTGAGTTTTATGGAGAGGGATGCTTGCCTTTTCCACTGTTACATACCCTTTTGTTTCAAGACATGCAACATTGGAAGGAGTGGTCTCCTTGTGAATCATATCAAGGAATTGGAGTTTTCCCTTGTATGACAAAGCTGACTATTAAACGATGCCCGGCATTGGAGGGTAGGCTACCCGAGGACCTTGATTCATTAGAAAAGCTTGAAATTGATGAGTGTGAAAATTTGATGGTTTCAATTGCCAACTACAAACAACTTCGTAATATAGACATCCACGGTTGCAAAGGGATGGTGCATTCAAGTGCAGTTGAATTTCGGTTACTAGAGTCCATGCATCTTTCAGATATTTTGAAGTTGACACTCCGAGCAGAGAGATTCATGAGAGGATTATCAATGGTTAAAGATTTAAAGATCACCGGTTGTGAGGAGCTGACGTCTTCATGGCAAAATGAGGATAGATTACTGCAACACCTAGTTTCTCTTCGTCGTTTGCTCATTAAAGGCAACTCCAACCTTGTTCAATTGCATCACTTGACATCACTTCAAGAACTTCACATAGATGAATGCTCAAATCTGGTTTCTTTTACAGAGGCTAGTTTGCCACATTCTCTTAAAGTTCTAACAATTGAGTCGTGCCCTTCTTTGATGTATCTTGCCAGAGACCAGATACCCCCAAGTCTAAGAAGAATAATTATACGAAGATGCCAAAATTTGAAATCCTTAGTGGAGGTTGTGATGGAGGGTTCTTTGTCTTCTTTTCCTTCTCTGATGCAAGAGGAGACATCTTGTCTTGAGTACTTGAGCATTGATATCTGTCCATCTCTGACGTCCTTATCATCCAGAGGCCATCTACCCAAGACGCTTAAACACCTTCTCATAGATACTTGTAAACAGCTGGAGTCAATAACAGAGAGGTTCGAGGAAAACACATGTCTTGAATATCTTTGCATCTATTTGTGTCAAAATCTTAAATTCTTACCAGAAGGCCTATGTAACCTCTCCAAAATTCAGTCGTTATTGATTTATGGCTGTGGAAATCTCGTTTCCTTCCCAATCGGAGGGTTGCCAAGAAGCGCCTCCAACCTGACAGAGATCAGCATTATCAATTGTGATAAACTGGAGGCTCTACCAGAAGGCATTAACTTCCTCAACTCTCTTCAGATATTGAGGATTCCTCACTTTCCAGAAGGGGGATTCCCTCCCTATCTAACATCACTTAGCATAATGAATCTCAAGATTTGTAGGCCACTGTTGGAGTGGGGTTTGCACAGACTCACATCTCTTAGAGTGTTATGGATCAGTGGTGAAGATCCAGATTTGGTGTCCTTTCCACCGGAGAAGGAGATGCTGCTCCCTGAATCGCTAGTTCAACTTGACATTATAGGCTTCCCCAATCTGAAGTACCTAGGCTTGCAGTTCCTCAACTCTCTTGAATCTCTTAAAATCTGGAGCTGTCCAAAGCTGACATCCATTCCAGAGGAAGGTCTGGCTCTTTCGCTTACACAGCTTAGTATCCGTGAGTGTCCTGTGCTAGAAGAGAAATGCAAACCAGGTAAAGGGCAGTACTGGCCCTCCATAAGCCACATCCCTTACGTATGGATTGGAGGCCGGAAGGTAACAACACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1350

Amino Acids

152.91

Weight (kDa)

6.42

Isoelectric Point (pI)

48.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 2858, 3251
Acc36I ACCTGC 1 cut(s) 788
AccB7I CCANNNNNTGG 2 cut(s) 497, 2357
AccBSI CCGCTC 1 cut(s) 146
AciI CCGC 7 cut(s) 146, 161, 1095, 1371, 1918, 1964, 2259
AclWI GGATC 4 cut(s) 866, 2282, 3725, 3725
AcoI YGGCCR 1 cut(s) 1092
AcuI CTGAAG 4 cut(s) 851, 1362, 3563, 3839
AcvI CACGTG 1 cut(s) 920
AcyI GRCGYC 2 cut(s) 2855, 3248
AfaI GTAC 6 cut(s) 315, 1029, 1941, 3218, 3824, 3986
AfiI CCNNNNNNNGG 8 cut(s) 497, 1970, 2357, 2581, 3479, 3611, 3902, 3977
AflIII ACRYGT 6 cut(s) 240, 839, 917, 1490, 2208, 3350
AgeI ACCGGT 2 cut(s) 51, 2837
AhdI GACNNNNNGTC 1 cut(s) 3207
AjiI CACGTC 1 cut(s) 735
AjnI CCWGG 2 cut(s) 2401, 3970
Alw21I GWGCWC 3 cut(s) 1716, 1746, 2313
Alw26I GTCTC 4 cut(s) 2378, 2511, 3075, 3194
AlwI GGATC 4 cut(s) 866, 2282, 3725, 3725
Ama87I CYCGRG 1 cut(s) 2597
ApeKI GCWGC 6 cut(s) 26, 43, 151, 1009, 1478, 3769
ArsI GACNNNNNNTTYG 2 cut(s) 1268, 1300
AseI ATTAAT 1 cut(s) 1911
AsiGI ACCGGT 2 cut(s) 51, 2837
AspA2I CCTAGG 1 cut(s) 3826
AspLEI GCGC 3 cut(s) 276, 1885, 3497
AspS9I GGNCC 4 cut(s) 488, 2048, 2602, 3990
AsuC2I CCSGG 2 cut(s) 739, 2576
AsuHPI GGTGA 8 cut(s) 382, 456, 521, 1385, 1574, 1772, 2827, 3737
AvaI CYCGRG 1 cut(s) 2597
AvaII GGWCC 2 cut(s) 488, 2602
AvrII CCTAGG 1 cut(s) 3826
AxyI CCTNAGG 1 cut(s) 663
BaeGI GKGCMC 1 cut(s) 3059
BanII GRGCYC 1 cut(s) 1746
BbrPI CACGTG 1 cut(s) 920
BbsI GAAGAC 4 cut(s) 243, 611, 2850, 3165
Bbv12I GWGCWC 3 cut(s) 1716, 1746, 2313
BbvCI CCTCAGC 1 cut(s) 2109
BbvI GCAGC 6 cut(s) 13, 30, 138, 1021, 1465, 3756
BciT130I CCWGG 2 cut(s) 2403, 3972
BciVI GTATCC 1 cut(s) 3944
BcnI CCSGG 2 cut(s) 739, 2576
BcoDI GTCTC 4 cut(s) 2378, 2511, 3075, 3194
BfmI CTRYAG 2 cut(s) 1479, 1844
BfoI RGCGCY 2 cut(s) 1886, 3498
BfuAI ACCTGC 1 cut(s) 788
BfuI GTATCC 1 cut(s) 3944
BglI GCCNNNNNGGC 1 cut(s) 2056
BglII AGATCT 1 cut(s) 15
BisI GCNGC 8 cut(s) 27, 44, 152, 1010, 1095, 1479, 1919, 3770
BlnI CCTAGG 1 cut(s) 3826
BlpI GCTNAGC 1 cut(s) 618
BlsI GCNGC 8 cut(s) 28, 45, 153, 1011, 1096, 1480, 1920, 3771
BmcAI AGTACT 2 cut(s) 3218, 3986
Bme1390I CCNGG 4 cut(s) 739, 2403, 2576, 3972
Bme18I GGWCC 2 cut(s) 488, 2602
BmeRI GACNNNNNGTC 1 cut(s) 3207
BmeT110I CYCGRG 1 cut(s) 2597
BmgBI CACGTC 1 cut(s) 735
BmgT120I GGNCC 4 cut(s) 488, 2048, 2602, 3990
BmrFI CCNGG 4 cut(s) 739, 2403, 2576, 3972
BpiI GAAGAC 4 cut(s) 243, 611, 2850, 3165
BplI GAGNNNNNCTC 2 cut(s) 3580, 3612
BpmI CTGGAG 5 cut(s) 615, 2424, 3338, 3560, 3889
Bpu10I CCTNAGC 1 cut(s) 2109
Bpu1102I GCTNAGC 1 cut(s) 618
BpuEI CTTGAG 6 cut(s) 1726, 1918, 2444, 3233, 3241, 3638
BpuMI CCSGG 2 cut(s) 739, 2576
Bsa29I ATCGAT 1 cut(s) 382
BsaAI YACGTR 2 cut(s) 920, 4015
BsaHI GRCGYC 2 cut(s) 2855, 3248
BsaI GGTCTC 2 cut(s) 2511, 3075
BsaJI CCNNGG 3 cut(s) 2598, 2695, 3826
BsaWI WCCGGW 4 cut(s) 51, 1559, 2837, 3754
BsaXI ACNNNNNCTCC 4 cut(s) 2840, 2870, 3977, 4007
Bsc4I CCNNNNNNNGG 8 cut(s) 497, 1970, 2357, 2581, 3479, 3611, 3902, 3977
Bse118I RCCGGY 2 cut(s) 51, 2837
Bse1I ACTGG 4 cut(s) 491, 1199, 3543, 3992
Bse21I CCTNAGG 1 cut(s) 663
Bse3DI GCAATG 1 cut(s) 2072
BseBI CCWGG 2 cut(s) 2403, 3972
BseCI ATCGAT 1 cut(s) 382
BseDI CCNNGG 3 cut(s) 2598, 2695, 3826
BseLI CCNNNNNNNGG 8 cut(s) 497, 1970, 2357, 2581, 3479, 3611, 3902, 3977
BseMI GCAATG 1 cut(s) 2072
BseMII CTCAG 8 cut(s) 252, 372, 632, 773, 1731, 1799, 2097, 2100
BseNI ACTGG 4 cut(s) 491, 1199, 3543, 3992
BseRI GAGGAG 3 cut(s) 51, 2861, 3212
BseSI GKGCMC 1 cut(s) 3059
BseXI GCAGC 6 cut(s) 13, 30, 138, 1021, 1465, 3756
BseYI CCCAGC 1 cut(s) 2281
BsgI GTGCAG 1 cut(s) 2745
Bsh1285I CGRYCG 1 cut(s) 52
BshTI ACCGGT 2 cut(s) 51, 2837
BshVI ATCGAT 1 cut(s) 382
BsiEI CGRYCG 1 cut(s) 52
BsiHKAI GWGCWC 3 cut(s) 1716, 1746, 2313
BsiHKCI CYCGRG 1 cut(s) 2597
BsiSI CCGG 7 cut(s) 52, 739, 1560, 2576, 2838, 3755, 4030
BslFI GGGAC 1 cut(s) 721
BslI CCNNNNNNNGG 8 cut(s) 497, 1970, 2357, 2581, 3479, 3611, 3902, 3977
BsmAI GTCTC 4 cut(s) 2378, 2511, 3075, 3194
BsmFI GGGAC 1 cut(s) 721
BsmI GAATGC 5 cut(s) 29, 1016, 2403, 2716, 2990
Bso31I GGTCTC 2 cut(s) 2511, 3075
BsoBI CYCGRG 1 cut(s) 2597
Bsp1286I GDGCHC 4 cut(s) 1716, 1746, 2313, 3059
Bsp1407I TGTACA 1 cut(s) 1027
Bsp1720I GCTNAGC 1 cut(s) 618
BspACI CCGC 7 cut(s) 146, 161, 1095, 1371, 1918, 1964, 2259
BspCNI CTCAG 8 cut(s) 253, 371, 631, 774, 1732, 1800, 2096, 2101
BspDI ATCGAT 1 cut(s) 382
BspHI TCATGA 3 cut(s) 1678, 2023, 2799
BspMAI CTGCAG 1 cut(s) 1483
BspMI ACCTGC 1 cut(s) 788
BspPI GGATC 4 cut(s) 866, 2282, 3725, 3725
BspTNI GGTCTC 2 cut(s) 2511, 3075
BsrBI CCGCTC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 2072
BsrFI RCCGGY 2 cut(s) 51, 2837
BsrGI TGTACA 1 cut(s) 1027
BsrI ACTGG 4 cut(s) 491, 1199, 3543, 3992
BssAI RCCGGY 2 cut(s) 51, 2837
BssECI CCNNGG 3 cut(s) 2598, 2695, 3826
BssNI GRCGYC 2 cut(s) 2855, 3248
BssT1I CCWWGG 1 cut(s) 3826
Bst2UI CCWGG 2 cut(s) 2403, 3972
Bst4CI ACNGT 7 cut(s) 198, 347, 746, 1473, 2460, 2699, 3672
Bst6I CTCTTC 4 cut(s) 496, 2907, 3582, 3951
BstACI GRCGYC 2 cut(s) 2855, 3248
BstAUI TGTACA 1 cut(s) 1027
BstBAI YACGTR 2 cut(s) 920, 4015
BstC8I GCNNGC 7 cut(s) 454, 615, 1014, 1514, 1958, 2447, 3833
BstDSI CCRYGG 1 cut(s) 2695
BstEII GGTNACC 1 cut(s) 462
BstH2I RGCGCY 2 cut(s) 1886, 3498
BstHHI GCGC 3 cut(s) 276, 1885, 3497
BstMAI GTCTC 4 cut(s) 2378, 2511, 3075, 3194
BstMCI CGRYCG 1 cut(s) 52
BstNI CCWGG 2 cut(s) 2403, 3972
BstPI GGTNACC 1 cut(s) 462
BstSCI CCNGG 4 cut(s) 737, 2401, 2574, 3970
BstSFI CTRYAG 2 cut(s) 1479, 1844
BstSLI GKGCMC 1 cut(s) 3059
BstSNI TACGTA 1 cut(s) 4015
BstV1I GCAGC 6 cut(s) 13, 30, 138, 1021, 1465, 3756
BstV2I GAAGAC 4 cut(s) 243, 611, 2850, 3165
BstX2I RGATCY 3 cut(s) 15, 2287, 3730
BstYI RGATCY 3 cut(s) 15, 2287, 3730
Bsu15I ATCGAT 1 cut(s) 382
Bsu36I CCTNAGG 1 cut(s) 663
BsuI GTATCC 1 cut(s) 3944
BsuTUI ATCGAT 1 cut(s) 382
BtgI CCRYGG 1 cut(s) 2695
BtrI CACGTC 1 cut(s) 735
BtsIMutI CAGTG 5 cut(s) 258, 742, 2456, 3668, 3727
BveI ACCTGC 1 cut(s) 788
Cac8I GCNNGC 7 cut(s) 454, 615, 1014, 1514, 1958, 2447, 3833
CciI TCATGA 3 cut(s) 1678, 2023, 2799
CfoI GCGC 3 cut(s) 276, 1885, 3497
Cfr10I RCCGGY 2 cut(s) 51, 2837
Cfr13I GGNCC 4 cut(s) 488, 2048, 2602, 3990
ClaI ATCGAT 1 cut(s) 382
CseI GACGC 1 cut(s) 3289
CsiI ACCWGGT 1 cut(s) 3970
Csp6I GTAC 6 cut(s) 314, 1028, 1940, 3217, 3823, 3985
CspAI ACCGGT 2 cut(s) 51, 2837
CviQI GTAC 6 cut(s) 314, 1028, 1940, 3217, 3823, 3985
DraI TTTAAA 2 cut(s) 423, 2829
DriI GACNNNNNGTC 1 cut(s) 3207
EaeI YGGCCR 1 cut(s) 1092
Eam1104I CTCTTC 4 cut(s) 496, 2907, 3582, 3951
Eam1105I GACNNNNNGTC 1 cut(s) 3207
EarI CTCTTC 4 cut(s) 496, 2907, 3582, 3951
Ecl136II GAGCTC 1 cut(s) 1744
Eco105I TACGTA 1 cut(s) 4015
Eco130I CCWWGG 1 cut(s) 3826
Eco147I AGGCCT 2 cut(s) 1592, 3408
Eco24I GRGCYC 1 cut(s) 1746
Eco31I GGTCTC 2 cut(s) 2511, 3075
Eco32I GATATC 2 cut(s) 396, 3232
Eco47I GGWCC 2 cut(s) 488, 2602
Eco53kI GAGCTC 1 cut(s) 1744
Eco57I CTGAAG 4 cut(s) 851, 1362, 3563, 3839
Eco72I CACGTG 1 cut(s) 920
Eco81I CCTNAGG 1 cut(s) 663
Eco88I CYCGRG 1 cut(s) 2597
Eco91I GGTNACC 1 cut(s) 462
EcoICRI GAGCTC 1 cut(s) 1744
EcoO109I RGGNCCY 1 cut(s) 2602
EcoO65I GGTNACC 1 cut(s) 462
EcoRI GAATTC 1 cut(s) 758
EcoRII CCWGG 2 cut(s) 2401, 3970
EcoRV GATATC 2 cut(s) 396, 3232
EcoT14I CCWWGG 1 cut(s) 3826
EcoT22I ATGCAT 3 cut(s) 1018, 1449, 2757
EcoT38I GRGCYC 1 cut(s) 1746
ErhI CCWWGG 1 cut(s) 3826
FaqI GGGAC 1 cut(s) 721
FauNDI CATATG 1 cut(s) 1545
Fnu4HI GCNGC 8 cut(s) 27, 44, 152, 1010, 1095, 1479, 1919, 3770
FriOI GRGCYC 1 cut(s) 1746
Fsp4HI GCNGC 8 cut(s) 27, 44, 152, 1010, 1095, 1479, 1919, 3770
GlaI GCGC 3 cut(s) 275, 1884, 3496
GluI GCNGC 8 cut(s) 27, 44, 152, 1010, 1095, 1479, 1919, 3770
GsaI CCCAGC 1 cut(s) 2285
GsuI CTGGAG 5 cut(s) 615, 2424, 3338, 3560, 3889
HaeII RGCGCY 2 cut(s) 1886, 3498
HapII CCGG 7 cut(s) 52, 739, 1560, 2576, 2838, 3755, 4030
HgaI GACGC 1 cut(s) 3289
HhaI GCGC 3 cut(s) 276, 1885, 3497
Hin1I GRCGYC 2 cut(s) 2855, 3248
Hin6I GCGC 3 cut(s) 274, 1883, 3495
HinP1I GCGC 3 cut(s) 274, 1883, 3495
HincII GTYRAC 2 cut(s) 57, 2778
HindII GTYRAC 2 cut(s) 57, 2778
HindIII AAGCTT 2 cut(s) 319, 2621
HpaII CCGG 7 cut(s) 52, 739, 1560, 2576, 2838, 3755, 4030
HphI GGTGA 8 cut(s) 382, 456, 521, 1385, 1574, 1772, 2827, 3737
Hpy166II GTNNAC 3 cut(s) 57, 2778, 3311
Hpy8I GTNNAC 3 cut(s) 57, 2778, 3311
Hpy99I CGWCG 1 cut(s) 2910
HpyCH4III ACNGT 7 cut(s) 198, 347, 746, 1473, 2460, 2699, 3672
HpyCH4IV ACGT 7 cut(s) 734, 919, 1209, 1850, 2855, 3248, 4014
HpySE526I ACGT 7 cut(s) 734, 919, 1209, 1850, 2855, 3248, 4014
Hsp92I GRCGYC 2 cut(s) 2855, 3248
HspAI GCGC 3 cut(s) 274, 1883, 3495
LmnI GCTCC 5 cut(s) 143, 887, 2848, 3777, 3870
Lsp1109I GCAGC 6 cut(s) 13, 30, 138, 1021, 1465, 3756
MabI ACCWGGT 1 cut(s) 3970
MaeII ACGT 7 cut(s) 734, 919, 1209, 1850, 2855, 3248, 4014
MaeIII GTNAC 9 cut(s) 462, 706, 842, 910, 2211, 2460, 2739, 3413, 4036
MbiI CCGCTC 1 cut(s) 146
MfeI CAATTG 8 cut(s) 138, 1755, 1976, 2326, 2658, 2942, 3045, 3526
MflI RGATCY 3 cut(s) 15, 2287, 3730
MhlI GDGCHC 4 cut(s) 1716, 1746, 2313, 3059
MlyI GAGTC 7 cut(s) 52, 1096, 2036, 2756, 3059, 3329, 3687
MmeI TCCRAC 6 cut(s) 478, 865, 974, 2955, 3525, 3654
Mph1103I ATGCAT 3 cut(s) 1018, 1449, 2757
MslI CAYNNNNRTG 4 cut(s) 263, 2167, 2721, 2979
MspA1I CMGCKG 1 cut(s) 3316
MspI CCGG 7 cut(s) 52, 739, 1560, 2576, 2838, 3755, 4030
MspR9I CCNGG 4 cut(s) 739, 2403, 2576, 3972
MunI CAATTG 8 cut(s) 138, 1755, 1976, 2326, 2658, 2942, 3045, 3526
Mva1269I GAATGC 5 cut(s) 29, 1016, 2403, 2716, 2990
MvaI CCWGG 2 cut(s) 2403, 3972
NciI CCSGG 2 cut(s) 739, 2576
NdeI CATATG 1 cut(s) 1545
NmuCI GTSAC 3 cut(s) 462, 842, 910
NsiI ATGCAT 3 cut(s) 1018, 1449, 2757
PaeI GCATGC 3 cut(s) 1016, 1516, 1960
PagI TCATGA 3 cut(s) 1678, 2023, 2799
PceI AGGCCT 2 cut(s) 1592, 3408
PciI ACATGT 5 cut(s) 240, 839, 1490, 2208, 3350
PctI GAATGC 5 cut(s) 29, 1016, 2403, 2716, 2990
PflMI CCANNNNNTGG 2 cut(s) 497, 2357
PinAI ACCGGT 2 cut(s) 51, 2837
PkrI GCNGC 8 cut(s) 28, 45, 153, 1011, 1096, 1480, 1920, 3771
PleI GAGTC 7 cut(s) 52, 1096, 2035, 2755, 3058, 3328, 3687
PmaCI CACGTG 1 cut(s) 920
PmlI CACGTG 1 cut(s) 920
PpsI GAGTC 7 cut(s) 52, 1096, 2035, 2755, 3058, 3328, 3687
Ppu21I YACGTR 2 cut(s) 920, 4015
PpuMI RGGWCCY 1 cut(s) 2602
PscI ACATGT 5 cut(s) 240, 839, 1490, 2208, 3350
PshBI ATTAAT 1 cut(s) 1911
Psp124BI GAGCTC 1 cut(s) 1746
Psp5II RGGWCCY 1 cut(s) 2602
Psp6I CCWGG 2 cut(s) 2401, 3970
PspCI CACGTG 1 cut(s) 920
PspEI GGTNACC 1 cut(s) 462
PspFI CCCAGC 1 cut(s) 2281
PspGI CCWGG 2 cut(s) 2401, 3970
PspPI GGNCC 4 cut(s) 488, 2048, 2602, 3990
PspPPI RGGWCCY 1 cut(s) 2602
PstI CTGCAG 1 cut(s) 1483
PsuI RGATCY 3 cut(s) 15, 2287, 3730
PvuII CAGCTG 1 cut(s) 3316
RsaI GTAC 6 cut(s) 315, 1029, 1941, 3218, 3824, 3986
RsaNI GTAC 6 cut(s) 314, 1028, 1940, 3217, 3823, 3985
RseI CAYNNNNRTG 4 cut(s) 263, 2167, 2721, 2979
SacI GAGCTC 1 cut(s) 1746
SatI GCNGC 8 cut(s) 27, 44, 152, 1010, 1095, 1479, 1919, 3770
Sau96I GGNCC 4 cut(s) 488, 2048, 2602, 3990
ScaI AGTACT 2 cut(s) 3218, 3986
SchI GAGTC 7 cut(s) 52, 1096, 2036, 2756, 3059, 3329, 3687
ScrFI CCNGG 4 cut(s) 739, 2403, 2576, 3972
SduI GDGCHC 4 cut(s) 1716, 1746, 2313, 3059
SexAI ACCWGGT 1 cut(s) 3970
SfcI CTRYAG 2 cut(s) 1479, 1844
SinI GGWCC 2 cut(s) 488, 2602
SmiMI CAYNNNNRTG 4 cut(s) 263, 2167, 2721, 2979
SmlI CTYRAG 6 cut(s) 1705, 1897, 2423, 3212, 3220, 3653
SmoI CTYRAG 6 cut(s) 1705, 1897, 2423, 3212, 3220, 3653
SnaBI TACGTA 1 cut(s) 4015
SphI GCATGC 3 cut(s) 1016, 1516, 1960
SseBI AGGCCT 2 cut(s) 1592, 3408
SsiI CCGC 7 cut(s) 146, 161, 1095, 1371, 1918, 1964, 2259
SspI AATATT 1 cut(s) 1042
SstI GAGCTC 1 cut(s) 1746
StuI AGGCCT 2 cut(s) 1592, 3408
StyD4I CCNGG 4 cut(s) 737, 2401, 2574, 3970
StyI CCWWGG 1 cut(s) 3826
TaaI ACNGT 7 cut(s) 198, 347, 746, 1473, 2460, 2699, 3672
TaiI ACGT 7 cut(s) 737, 922, 1212, 1853, 2858, 3251, 4017
TaqI TCGA 3 cut(s) 48, 382, 3339
TatI WGTACW 3 cut(s) 1027, 3216, 3984
TauI GCSGC 2 cut(s) 1097, 1921
TscAI CASTG 5 cut(s) 265, 749, 2463, 3675, 3727
TseFI GTSAC 3 cut(s) 462, 842, 910
TseI GCWGC 6 cut(s) 26, 43, 151, 1009, 1478, 3769
Tsp45I GTSAC 3 cut(s) 462, 842, 910
TspGWI ACGGA 2 cut(s) 667, 3926
TspRI CASTG 5 cut(s) 265, 749, 2463, 3675, 3727
Van91I CCANNNNNTGG 2 cut(s) 497, 2357
VpaK11BI GGWCC 2 cut(s) 488, 2602
VspI ATTAAT 1 cut(s) 1911
XbaI TCTAGA 1 cut(s) 2222
XcmI CCANNNNNNNNNTGG 1 cut(s) 3675
XmaJI CCTAGG 1 cut(s) 3826
ZraI GACGTC 2 cut(s) 2856, 3249
ZrmI AGTACT 2 cut(s) 3218, 3986
Zsp2I ATGCAT 3 cut(s) 1018, 1449, 2757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.