RchiOBHm_Chr1g0334991

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000070
Physical Location & Seq
Reverse (-)
27208680 .. 27211627
2948 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56367

Sequence Viewer

Length: 2883 bp
ATGGCAGTGGGAGAAGTCTTTCTTGCGGCATTCCTTCAGTTGCTGCTGAACAGGTTAACGCCTCGGGAGATCCTGGGCTACTTTGGAAGCTTGGGAGGAGTTGGTAAAAAGCTGCAGAAATTGAGGGAAACACTGTCCGCAGTTGCAGCGGTGCTTTATGACGCAGAGGATAAGCAGCTGTCGAGCAAGGCAGTGGATCTGTGGCTGAATGATCTCAGACACTTGGCTTACGATATCGACGATCTACTCAACACCTTTTCTACAGAGATGTTAACACGCAAGCAGCTCAAGGTTCACCAAACTGGCACAAGCAAGGTACGAGGCTTCTTTACCAAAGTTCCTCACAAAGTTAAGTTCAATTTCAATATGAAGTCCGAAATAGACGAGATTGCTAACCGCTTGCAAGACATATTTGACCGGAAAGAGAAACTTGGTTTGAAATATATTGAGCATGCCTCTACTTCAACATCATCATTGCAAAGGACACCAAGTTCGTGTGTGTTGGATGGACCTGTGGTTGGGAGAGAGGAAGACGCAAGAAAAATAGTGGAGTTGTTGTCCAGGGATGCAGATCCTTCTTCTCCCACAAATTATGAGGTTGCTGCCATTGTTGGTATGGGAGGACTGGGCAAGACAACGCTTGCGGGACAAGTCTTCAATGATGTAGCTACAATGGAACAATTTGATCTCAGGATCTGGGTTTCAGTATCCGATGACTTCGATCTTCAAATGGTGACGAGAACTATTTCTGAAAACGTCACATCTAGGCCATGTGATGATTTCAGTCGACTTCAAGATAATCTGAGCAAGGCGATAGATGGTAAAAAGTTTTTGATTGTTTTAGATGATGTCTGGAGCACGTGTGGTTATGATTCATGGACAAAACTGCAAGCTCCCTTCCGTGGGGGAGCTAAGGGAAGTAGGATACTGGTGACAACACGTGATGAAAAGGTGGCAGCATTGATGGGAGCCCCAGCCGCTGAAGTTTATTATTTAAATACTCTATCGGATGAAGGTTGTTTGCAACTATTTGAACAGCATGTTAGCAATGACAGGCCACCGAATTTTGACTTGCTTAAGAAGAAAATTATTACGAAATGCAATGGATTACCATTGGCTGCAAAAACTCTTGGTGGTGTTTTACGCTGTAAAGAAACTGACAAATGGGAGGAAATACTGAATGACAAGTTATGGAGTATATCAGATGGGAGTAATATACTCCCAGTACTCAGACTGAGTTATAATTATCTCCCTTCAACTTTGAAGAGGTGCTTTGCCTATTGCTCAGTACTTCCCAATAATTATGAATTTGGGAAAAACCAATTAATCCTTCTATGGATGGCAGAGGGTTTCCTTGAGCAACCAGAAGGAACCAAAGCAATGGAAGATATTGGTGACGAATATTTTGGGGAGCTATTGTCTCGGTCATTATTTCAGAATTCGGGCAAAAACAGTTCATACTATGTAATGCATGACCTTGTTGGTGATTTGGCACGATGGGCTGCTGGAGACACATTTTGTAGATTAGAGGATAAACCGCATGGTAGATGTTCTCCAACGACTCGTCATCTGACTTACATTTCTGGTAAGTTCGATGGGATAAAACGATTTGAGACACTTTCTGAAGCAAAATGCTTGCGGACTTTCCTTCCACTTTTAGTGTCCAATGGTTATGAGAATTATCTAACTCGTTATGCTACTTCTGATTTATTGTCACAATTGAAGTACTTGCGGGTGCTCTCCTTCAATGGGTATAAACTGATCGAGGTACCAGATTCCGTATGTAATTTGAAACATCTACGCTACCTTGATCTTTCTCACACATTGATAATGAGTTTGCCTGAGTTAATATGCATGCTTTACAACTTACAGACGTTAATATTAGAGAATTGTTCAAAATTGAAGACACTACCTTCAAACATGAGCAATTTATCTAATTTACGTCATCTCAACAATTCAAATACGCCTTTATTGGAAGAAATGCCTCCCCAATTAAGTCGGTTGACTCATCTGCGAACTTTGCCTAATTTTGTGGTGGGGAAAGGTAGTGCATCAGGCATTGGAGAGTTAGGGTCATTGCATCTTCTTGGGACCTTGAGACTTGCAAGATTGGAGAATGTGATTGATGTTGAGGATGCAAAGAGGGCCCAAATAATGAACAAGGAAGCGCTGGAAACCTTGCGATTGGAATGGAGTGGCACAAGTGAGAAGGAGTCGGAGGTGCTCTGCAGTTTAAAACCTCAGAAAAAGCTTCTAGAGCTCACCATCAAGGGTTACAATGGTTTAGAATTCTCAAAATGGATTGGACATCCTTCATTCTCTGATATGACGTATGTGAAGTTACAGAACTGTAAAAATTGTCGATTCTTACCTCCTCTTGGCCAATTACCTTTACTAAAAGTCCTTTGGATAAAAGGACTGCCCAATGTTGAAAGTGTGGGTGCTGAGTTTTATGGAGATTGTAGCTTGCCTTTTCCAGTGTTGGAAGAGTTGAGGTTTGAGGATATGCAAAATTGGAAGGAGTGGCTTCCTTGCAAAAGAAATGAAGAAATTCGAATTTTCCCATGCATGGGAAAACTTTTCATCCGTAGTTGCCCCAGTCTGAAAGGTTGGTTGCCCAAGAACATGGATTCATTATCAAAGCTGCGTATTGTTGAATGTGAAGAGTTAGTGGTTTCAATTGCCAATTATAAACATCTTCGTGAGCTGCGCATCGACGGTTGCAAAAGGGTGGTGCACAGAAGGGGAGTTAAGTTTAAGTTACTGGAGGATATGCGGCTTCATAGTACTTCAGAGTTCAGACTCAAAATAGATGGGTTCATCAGAGGATTGACAAAGCTTCAAAGATTGTGGATTACTGGTTGTGAAGAGCAACATCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

960

Amino Acids

108.91

Weight (kDa)

8.32

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 99 3.2e-19 Rx N-terminal domain
NB-ARC PF00931 176 - 350 7.3e-36 NB-ARC domain
WHD_DRP PF23559 431 - 498 1.3e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 570 - 836 2.1e-26 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 687 - 806 2.7e-31 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1242, 2691
Acc16I TGCGCA 1 cut(s) 2711
Acc65I GGTACC 1 cut(s) 1768
AccB1I GGYRCC 1 cut(s) 1768
AccI GTMKAC 1 cut(s) 787
AclWI GGATC 4 cut(s) 64, 204, 566, 701
AcoI YGGCCR 1 cut(s) 2380
AcsI RAATTY 6 cut(s) 1063, 1307, 1438, 2288, 2550, 2556
AcuI CTGAAG 4 cut(s) 20, 1002, 1644, 2775
AcvI CACGTG 2 cut(s) 861, 941
AfaI GTAC 6 cut(s) 318, 1227, 1290, 1727, 1770, 2788
AfeI AGCGCT 1 cut(s) 2169
AfiI CCNNNNNNNGG 7 cut(s) 518, 902, 903, 1749, 2378, 2569, 2570
AflII CTTAAG 1 cut(s) 1076
AflIII ACRYGT 2 cut(s) 860, 938
AjnI CCWGG 2 cut(s) 72, 560
AjuI GAANNNNNNNTTGG 2 cut(s) 1314, 1346
AloI GAACNNNNNNTCC 2 cut(s) 475, 507
Alw21I GWGCWC 5 cut(s) 860, 1740, 2226, 2262, 2739
Alw26I GTCTC 4 cut(s) 1425, 1503, 1607, 2092
Alw44I GTGCAC 1 cut(s) 2735
AlwI GGATC 4 cut(s) 64, 204, 566, 701
AlwNI CAGNNNCTG 3 cut(s) 43, 696, 980
Ama87I CYCGRG 1 cut(s) 63
Aor51HI AGCGCT 1 cut(s) 2169
AoxI GGCC 4 cut(s) 767, 1055, 2145, 2380
ApaI GGGCCC 1 cut(s) 2149
ApaLI GTGCAC 1 cut(s) 2735
ApoI RAATTY 6 cut(s) 1063, 1307, 1438, 2288, 2550, 2556
ArsI GACNNNNNNTTYG 4 cut(s) 475, 507, 1388, 1420
AseI ATTAAT 1 cut(s) 1325
Asp700I GAANNNNTTC 3 cut(s) 18, 745, 2550
Asp718I GGTACC 1 cut(s) 1768
AspLEI GCGC 2 cut(s) 2170, 2712
AspS9I GGNCC 4 cut(s) 509, 2091, 2145, 2146
AsuHPI GGTGA 6 cut(s) 287, 745, 943, 1406, 1496, 2254
AsuII TTCGAA 1 cut(s) 2554
AvaI CYCGRG 1 cut(s) 63
AvaII GGWCC 2 cut(s) 509, 2091
BaeGI GKGCMC 2 cut(s) 2149, 2739
BalI TGGCCA 1 cut(s) 2382
BanI GGYRCC 1 cut(s) 1768
BanII GRGCYC 3 cut(s) 973, 2149, 2262
BbrPI CACGTG 2 cut(s) 861, 941
BbsI GAAGAC 3 cut(s) 537, 646, 1910
Bbv12I GWGCWC 5 cut(s) 860, 1740, 2226, 2262, 2739
BccI CCATC 9 cut(s) 500, 812, 958, 1199, 1333, 1491, 1589, 2273, 2807
BciT130I CCWGG 2 cut(s) 74, 562
BciVI GTATCC 2 cut(s) 718, 918
BcoDI GTCTC 4 cut(s) 1425, 1503, 1607, 2092
BfaI CTAG 2 cut(s) 765, 2255
BfmI CTRYAG 3 cut(s) 113, 261, 2227
BfoI RGCGCY 1 cut(s) 2171
BfrI CTTAAG 1 cut(s) 1076
BfuI GTATCC 2 cut(s) 718, 918
BmcAI AGTACT 4 cut(s) 1227, 1290, 1727, 2788
Bme1390I CCNGG 2 cut(s) 74, 562
Bme18I GGWCC 2 cut(s) 509, 2091
BmeT110I CYCGRG 1 cut(s) 63
BmgT120I GGNCC 4 cut(s) 509, 2091, 2145, 2146
BmiI GGNNCC 5 cut(s) 970, 1372, 1770, 2092, 2147
BmrFI CCNGG 2 cut(s) 74, 562
BmrI ACTGGG 3 cut(s) 635, 1217, 2592
BmsI GCATC 5 cut(s) 556, 2060, 2089, 2125, 2721
BmuI ACTGGG 3 cut(s) 635, 1217, 2592
BpiI GAAGAC 3 cut(s) 537, 646, 1910
BplI GAGNNNNNCTC 2 cut(s) 440, 472
BpmI CTGGAG 3 cut(s) 874, 1527, 2786
Bpu10I CCTNAGC 1 cut(s) 912
Bpu14I TTCGAA 1 cut(s) 2554
BpuEI CTTGAG 3 cut(s) 272, 1376, 2116
BsaAI YACGTR 2 cut(s) 861, 941
BsaBI GATNNNNATC 1 cut(s) 570
BsaJI CCNNGG 4 cut(s) 62, 73, 561, 901
BsaWI WCCGGW 1 cut(s) 417
BsaXI ACNNNNNCTCC 6 cut(s) 542, 572, 847, 877, 1403, 1433
Bsc4I CCNNNNNNNGG 7 cut(s) 518, 902, 903, 1749, 2378, 2569, 2570
Bse1I ACTGG 8 cut(s) 307, 630, 933, 1223, 2477, 2598, 2769, 2863
Bse3DI GCAATG 5 cut(s) 473, 1054, 1108, 1386, 2075
Bse8I GATNNNNATC 1 cut(s) 570
BseBI CCWGG 2 cut(s) 74, 562
BseDI CCNNGG 4 cut(s) 62, 73, 561, 901
BseGI GGATG 7 cut(s) 511, 571, 1015, 1344, 2140, 2308, 2583
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 7 cut(s) 518, 902, 903, 1749, 2378, 2569, 2570
BseMI GCAATG 5 cut(s) 473, 1054, 1108, 1386, 2075
BseMII CTCAG 9 cut(s) 229, 703, 794, 1226, 1243, 1299, 1833, 2255, 2436
BseNI ACTGG 8 cut(s) 307, 630, 933, 1223, 2477, 2598, 2769, 2863
BseRI GAGGAG 2 cut(s) 111, 2364
BseSI GKGCMC 2 cut(s) 2149, 2739
BseYI CCCAGC 1 cut(s) 973
BshFI GGCC 4 cut(s) 769, 1057, 2147, 2382
BshNI GGYRCC 1 cut(s) 1768
BsiHKAI GWGCWC 5 cut(s) 860, 1740, 2226, 2262, 2739
BsiHKCI CYCGRG 1 cut(s) 63
BsiSI CCGG 1 cut(s) 418
BslFI GGGAC 2 cut(s) 660, 2104
BslI CCNNNNNNNGG 7 cut(s) 518, 902, 903, 1749, 2378, 2569, 2570
BsmAI GTCTC 4 cut(s) 1425, 1503, 1607, 2092
BsmFI GGGAC 2 cut(s) 660, 2104
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 4 cut(s) 769, 1057, 2147, 2382
BsoBI CYCGRG 1 cut(s) 63
Bsp119I TTCGAA 1 cut(s) 2554
Bsp120I GGGCCC 1 cut(s) 2145
Bsp1286I GDGCHC 7 cut(s) 860, 973, 1740, 2149, 2226, 2262, 2739
BspANI GGCC 4 cut(s) 769, 1057, 2147, 2382
BspCNI CTCAG 9 cut(s) 228, 702, 795, 1227, 1242, 1298, 1834, 2254, 2437
BspLI GGNNCC 5 cut(s) 970, 1372, 1770, 2092, 2147
BspMAI CTGCAG 2 cut(s) 117, 2231
BspPI GGATC 4 cut(s) 64, 204, 566, 701
BspQI GCTCTTC 1 cut(s) 2862
BspT104I TTCGAA 1 cut(s) 2554
BspT107I GGYRCC 1 cut(s) 1768
BspTI CTTAAG 1 cut(s) 1076
BsrDI GCAATG 5 cut(s) 473, 1054, 1108, 1386, 2075
BsrI ACTGG 8 cut(s) 307, 630, 933, 1223, 2477, 2598, 2769, 2863
BssECI CCNNGG 4 cut(s) 62, 73, 561, 901
Bst2UI CCWGG 2 cut(s) 74, 562
Bst4CI ACNGT 4 cut(s) 135, 1454, 2351, 2720
Bst6I CTCTTC 4 cut(s) 1259, 2481, 2658, 2862
BstAFI CTTAAG 1 cut(s) 1076
BstBAI YACGTR 2 cut(s) 861, 941
BstBI TTCGAA 1 cut(s) 2554
BstC8I GCNNGC 8 cut(s) 281, 401, 453, 642, 891, 1637, 1856, 2468
BstDSI CCRYGG 1 cut(s) 901
BstF5I GGATG 7 cut(s) 511, 571, 1015, 1344, 2140, 2308, 2583
BstH2I RGCGCY 1 cut(s) 2171
BstHHI GCGC 2 cut(s) 2170, 2712
BstMAI GTCTC 4 cut(s) 1425, 1503, 1607, 2092
BstMWI GCNNNNNNNGC 6 cut(s) 146, 977, 1499, 2020, 2144, 2257
BstNI CCWGG 2 cut(s) 74, 562
BstNSI RCATGY 3 cut(s) 455, 1043, 1858
BstSCI CCNGG 2 cut(s) 72, 560
BstSFI CTRYAG 3 cut(s) 113, 261, 2227
BstSLI GKGCMC 2 cut(s) 2149, 2739
BstV2I GAAGAC 3 cut(s) 537, 646, 1910
BstX2I RGATCY 4 cut(s) 69, 196, 571, 693
BstXI CCANNNNNNTGG 2 cut(s) 1381, 2626
BstYI RGATCY 4 cut(s) 69, 196, 571, 693
BsuI GTATCC 2 cut(s) 718, 918
BsuRI GGCC 4 cut(s) 769, 1057, 2147, 2382
BtgI CCRYGG 1 cut(s) 901
BtsCI GGATG 7 cut(s) 511, 571, 1015, 1344, 2140, 2308, 2583
BtsI GCAGTG 2 cut(s) 12, 198
BtsIMutI CAGTG 4 cut(s) 12, 131, 198, 2484
Cac8I GCNNGC 8 cut(s) 281, 401, 453, 642, 891, 1637, 1856, 2468
CaiI CAGNNNCTG 3 cut(s) 43, 696, 980
CfoI GCGC 2 cut(s) 2170, 2712
Cfr13I GGNCC 4 cut(s) 509, 2091, 2145, 2146
CseI GACGC 2 cut(s) 170, 542
Csp6I GTAC 6 cut(s) 317, 1226, 1289, 1726, 1769, 2787
CspCI CAANNNNNGTGG 2 cut(s) 2831, 2866
CviQI GTAC 6 cut(s) 317, 1226, 1289, 1726, 1769, 2787
DraI TTTAAA 2 cut(s) 996, 2235
EaeI YGGCCR 1 cut(s) 2380
Eam1104I CTCTTC 4 cut(s) 1259, 2481, 2658, 2862
EarI CTCTTC 4 cut(s) 1259, 2481, 2658, 2862
Ecl136II GAGCTC 1 cut(s) 2260
Eco24I GRGCYC 3 cut(s) 973, 2149, 2262
Eco32I GATATC 1 cut(s) 235
Eco47I GGWCC 2 cut(s) 509, 2091
Eco47III AGCGCT 1 cut(s) 2169
Eco53kI GAGCTC 1 cut(s) 2260
Eco57I CTGAAG 4 cut(s) 20, 1002, 1644, 2775
Eco72I CACGTG 2 cut(s) 861, 941
Eco88I CYCGRG 1 cut(s) 63
EcoICRI GAGCTC 1 cut(s) 2260
EcoO109I RGGNCCY 2 cut(s) 2091, 2145
EcoRI GAATTC 2 cut(s) 1438, 2288
EcoRII CCWGG 2 cut(s) 72, 560
EcoRV GATATC 1 cut(s) 235
EcoT22I ATGCAT 3 cut(s) 1473, 1856, 2570
EcoT38I GRGCYC 3 cut(s) 973, 2149, 2262
FalI AAGNNNNNCTT 5 cut(s) 38, 414, 446, 1256, 1288
FaqI GGGAC 2 cut(s) 660, 2104
FauI CCCGC 2 cut(s) 637, 1725
FblI GTMKAC 1 cut(s) 787
FokI GGATG 7 cut(s) 518, 578, 1022, 1351, 2147, 2295, 2570
FriOI GRGCYC 3 cut(s) 973, 2149, 2262
FspBI CTAG 2 cut(s) 765, 2255
FspI TGCGCA 1 cut(s) 2711
GlaI GCGC 2 cut(s) 2169, 2711
GsaI CCCAGC 1 cut(s) 977
GsuI CTGGAG 3 cut(s) 874, 1527, 2786
HaeII RGCGCY 1 cut(s) 2171
HaeIII GGCC 4 cut(s) 769, 1057, 2147, 2382
HapII CCGG 1 cut(s) 418
HgaI GACGC 2 cut(s) 170, 542
HhaI GCGC 2 cut(s) 2170, 2712
Hin6I GCGC 2 cut(s) 2168, 2710
HinP1I GCGC 2 cut(s) 2168, 2710
HincII GTYRAC 4 cut(s) 57, 273, 788, 2004
HindII GTYRAC 4 cut(s) 57, 273, 788, 2004
HindIII AAGCTT 3 cut(s) 88, 2249, 2837
HinfI GANTC 8 cut(s) 872, 1561, 1775, 2005, 2213, 2364, 2630, 2802
HpaI GTTAAC 2 cut(s) 57, 273
HpaII CCGG 1 cut(s) 418
HphI GGTGA 6 cut(s) 287, 745, 943, 1406, 1496, 2254
Hpy166II GTNNAC 6 cut(s) 57, 273, 295, 788, 2004, 2737
Hpy188III TCNNGA 6 cut(s) 65, 691, 794, 853, 2255, 2702
Hpy8I GTNNAC 6 cut(s) 57, 273, 295, 788, 2004, 2737
Hpy99I CGWCG 2 cut(s) 242, 2720
HpyCH4III ACNGT 4 cut(s) 135, 1454, 2351, 2720
HpyCH4IV ACGT 6 cut(s) 756, 860, 940, 1874, 1942, 2330
HpyF10VI GCNNNNNNNGC 6 cut(s) 146, 977, 1499, 2020, 2144, 2257
HpySE526I ACGT 6 cut(s) 756, 860, 940, 1874, 1942, 2330
HspAI GCGC 2 cut(s) 2168, 2710
KpnI GGTACC 1 cut(s) 1772
KspAI GTTAAC 2 cut(s) 57, 273
LguI GCTCTTC 1 cut(s) 2862
LmnI GCTCC 5 cut(s) 855, 898, 908, 968, 1411
LweI GCATC 5 cut(s) 556, 2060, 2089, 2125, 2721
MaeI CTAG 2 cut(s) 765, 2255
MaeII ACGT 6 cut(s) 756, 860, 940, 1874, 1942, 2330
MaeIII GTNAC 8 cut(s) 733, 757, 931, 1394, 1713, 2273, 2340, 2760
MfeI CAATTG 2 cut(s) 1718, 2679
MflI RGATCY 4 cut(s) 69, 196, 571, 693
MhlI GDGCHC 7 cut(s) 860, 973, 1740, 2149, 2226, 2262, 2739
MlsI TGGCCA 1 cut(s) 2382
MluNI TGGCCA 1 cut(s) 2382
MlyI GAGTC 4 cut(s) 1555, 1999, 2222, 2796
MmeI TCCRAC 4 cut(s) 483, 1580, 2196, 2463
Mox20I TGGCCA 1 cut(s) 2382
Mph1103I ATGCAT 3 cut(s) 1473, 1856, 2570
MroXI GAANNNNTTC 3 cut(s) 18, 745, 2550
MscI TGGCCA 1 cut(s) 2382
MslI CAYNNNNRTG 1 cut(s) 2700
Msp20I TGGCCA 1 cut(s) 2382
MspA1I CMGCKG 3 cut(s) 149, 178, 980
MspCI CTTAAG 1 cut(s) 1076
MspI CCGG 1 cut(s) 418
MspR9I CCNGG 2 cut(s) 74, 562
MunI CAATTG 2 cut(s) 1718, 2679
Mva1269I GAATGC 1 cut(s) 29
MvaI CCWGG 2 cut(s) 74, 562
MwoI GCNNNNNNNGC 6 cut(s) 146, 977, 1499, 2020, 2144, 2257
NlaIV GGNNCC 5 cut(s) 970, 1372, 1770, 2092, 2147
NmuCI GTSAC 5 cut(s) 733, 757, 931, 1394, 1713
NsbI TGCGCA 1 cut(s) 2711
NsiI ATGCAT 3 cut(s) 1473, 1856, 2570
NspI RCATGY 3 cut(s) 455, 1043, 1858
NspV TTCGAA 1 cut(s) 2554
PaeI GCATGC 2 cut(s) 455, 1858
PciSI GCTCTTC 1 cut(s) 2862
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PctI GAATGC 1 cut(s) 29
PdmI GAANNNNTTC 3 cut(s) 18, 745, 2550
PfeI GAWTC 4 cut(s) 872, 1775, 2364, 2630
PleI GAGTC 4 cut(s) 1555, 1999, 2221, 2796
PmaCI CACGTG 2 cut(s) 861, 941
PmlI CACGTG 2 cut(s) 861, 941
PpsI GAGTC 4 cut(s) 1555, 1999, 2221, 2796
Ppu21I YACGTR 2 cut(s) 861, 941
PpuMI RGGWCCY 1 cut(s) 2091
PshBI ATTAAT 1 cut(s) 1325
PsiI TTATAA 2 cut(s) 1242, 2691
Psp124BI GAGCTC 1 cut(s) 2262
Psp5II RGGWCCY 1 cut(s) 2091
Psp6I CCWGG 2 cut(s) 72, 560
PspCI CACGTG 2 cut(s) 861, 941
PspFI CCCAGC 1 cut(s) 973
PspGI CCWGG 2 cut(s) 72, 560
PspN4I GGNNCC 5 cut(s) 970, 1372, 1770, 2092, 2147
PspOMI GGGCCC 1 cut(s) 2145
PspPI GGNCC 4 cut(s) 509, 2091, 2145, 2146
PspPPI RGGWCCY 1 cut(s) 2091
PstI CTGCAG 2 cut(s) 117, 2231
PstNI CAGNNNCTG 3 cut(s) 43, 696, 980
PsuI RGATCY 4 cut(s) 69, 196, 571, 693
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 6 cut(s) 318, 1227, 1290, 1727, 1770, 2788
RsaNI GTAC 6 cut(s) 317, 1226, 1289, 1726, 1769, 2787
RseI CAYNNNNRTG 1 cut(s) 2700
SacI GAGCTC 1 cut(s) 2262
SalI GTCGAC 1 cut(s) 786
SapI GCTCTTC 1 cut(s) 2862
Sau96I GGNCC 4 cut(s) 509, 2091, 2145, 2146
ScaI AGTACT 4 cut(s) 1227, 1290, 1727, 2788
SchI GAGTC 4 cut(s) 1555, 1999, 2222, 2796
ScrFI CCNGG 2 cut(s) 74, 562
SduI GDGCHC 7 cut(s) 860, 973, 1740, 2149, 2226, 2262, 2739
SfaNI GCATC 5 cut(s) 556, 2060, 2089, 2125, 2721
SfcI CTRYAG 3 cut(s) 113, 261, 2227
SfuI TTCGAA 1 cut(s) 2554
SinI GGWCC 2 cut(s) 509, 2091
SmiI ATTTAAAT 1 cut(s) 996
SmiMI CAYNNNNRTG 1 cut(s) 2700
SmlI CTYRAG 4 cut(s) 287, 1076, 1355, 2095
SmoI CTYRAG 4 cut(s) 287, 1076, 1355, 2095
SphI GCATGC 2 cut(s) 455, 1858
SspI AATATT 2 cut(s) 1403, 1881
SspMI CTAG 2 cut(s) 765, 2255
SstI GAGCTC 1 cut(s) 2262
StyD4I CCNGG 2 cut(s) 72, 560
SwaI ATTTAAAT 1 cut(s) 996
TaaI ACNGT 4 cut(s) 135, 1454, 2351, 2720
TaiI ACGT 6 cut(s) 759, 863, 943, 1877, 1945, 2333
TaqI TCGA 9 cut(s) 182, 237, 720, 787, 1593, 1764, 2362, 2554, 2715
TaqII GACCGA 1 cut(s) 1413
TatI WGTACW 4 cut(s) 1225, 1288, 1725, 2786
TauI GCSGC 3 cut(s) 29, 980, 2779
TfiI GAWTC 4 cut(s) 872, 1775, 2364, 2630
TscAI CASTG 4 cut(s) 12, 138, 198, 2484
TseFI GTSAC 5 cut(s) 733, 757, 931, 1394, 1713
Tsp45I GTSAC 5 cut(s) 733, 757, 931, 1394, 1713
TspGWI ACGGA 3 cut(s) 890, 1768, 2576
TspRI CASTG 4 cut(s) 12, 138, 198, 2484
Vha464I CTTAAG 1 cut(s) 1076
VneI GTGCAC 1 cut(s) 2735
VpaK11BI GGWCC 2 cut(s) 509, 2091
VspI ATTAAT 1 cut(s) 1325
XapI RAATTY 6 cut(s) 1063, 1307, 1438, 2288, 2550, 2556
XbaI TCTAGA 1 cut(s) 2254
XceI RCATGY 3 cut(s) 455, 1043, 1858
XcmI CCANNNNNNNNNTGG 1 cut(s) 613
XmiI GTMKAC 1 cut(s) 787
XmnI GAANNNNTTC 3 cut(s) 18, 745, 2550
XspI CTAG 2 cut(s) 765, 2255
ZrmI AGTACT 4 cut(s) 1227, 1290, 1727, 2788
Zsp2I ATGCAT 3 cut(s) 1473, 1856, 2570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.