MD11G1033600.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000070
Physical Location & Seq
Reverse (-)
2912846 .. 2918842
5997 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1033600.v1.1.491

Sequence Viewer

Length: 4230 bp
ATGGCACTGGGAGAGATTTTTCTTGCGGCGTTTCTACAGTTGCTGCTCGACAGGTTGACCCCTCACGAGATTCTCAACTACTTTGGAAACTTCCGGGGCGTCAGAAGGAAGCTGGAGAAGTGGAGGACCACGTTATCTACAATCGGAGTGGTGGTGAGTGATGCCGAGGAAAGGCAACTGACTGAGGGTGCTGTAAAACTGTGGCTCGATGATCTCAGAGATTTGGCTTATGATATCGAAGACATGTTGGACAAATTTGCCACACAAATGTTGAAGCGCATGATAGAGAGACGTGAACAAGCCAACACAAGCAACAAGATACGGAGATCATTTTATAAAGTTAAATTGAGGTTTGATATGAACTCCGAAATGAAGAAGATTATGGAGCGGTTGCAAGACATATCTGAACGGAAAGAAAAGTTTGGCTTGAAAGATACTGGGACGTCTGCTAAGGAATCACGAAGTTTACAAAGTGCAGGCGTGTTAGATGAAAAGCTTGTTGTTGGAAGAGATGATGACAAAGGGGAGATTATCGAATTGTTGTCAAGAAAATACAAGCATGCAGGTACTGTCAATTTTGGTGTAGTTGCTATAGTTGGGATGCCCGGAGTCGGGAAGACGACACTTGCTCAACTTGTATTCAACCACAAAGATGATGCCATGAAGGAGTTTGAGCCAAAGGTGTGGGTATCTGTGTCTGATGACTTCGATGTTGTGCGAGTGACAAAGGCAATTCTTGAATCAATCACATCCCGACCCGTTCAAGTGGAGGAGTTTAGTAAAATGCAGCATGATTTGAGTGAGCAGTTAAGAGGAAAAAAGTTTTTAATCGTTTTAGATGATATTTGGAACAAAGGTGACTATGATCTATACGATCTCTGGACAAGACTTCAATCCCCTTTTGGCGTCGGAGCTGGAGGAAGTAAGATACTTGTGACAACCCGCGATGTGAATGTTGCAAAGTTTATGGGAGCCGCTGGAGTCCATAATTTGAAGTGCATGAGAGATGATGATTGTTTGGAAATATTTGAGCGACATGCATTTGGGGAACTTAATGATGGAAAACCAGTAAATTATGAGTTAATTCGAAGAAAAATTGTCGAAAAATGTCGTGGATTACCATTTGCTGCAAGGACTCTCGGTGGCCTTTTACGTTGCAAAGAAAAAGATGAGTGGGAAGAAATATTGAACAACAAATTGTGGAATATAGCAGATAAGAGTGACATTCTCCCCGTACTAAAGTTGAGCTATCACTATCTTCCATCAAATTTGAAGAGGTGTTTTGCCTATTGCTCAATACTTCCAAACGACTATGAATTTAGGGAGAAGCAACTCGTCCTTTTGTGGATGGCAGAGGGTTTGATTCAACAAAAACCTGAAGACAATAAACAAATGGAGGATTTGGGCCGTGACTACTTTCGAGAGCTATTATCAAGGTCGTTGTTTCAAGAATCAAGCAAAAACAATTCACAATTTGTAATGCATGACCTCGTTAATGATTTAGCACAATGGGCAGCAGGAGAAATATGTTCTAGGTTGGAAGATAAGCAAGGTGATAACTTGCAACGCACCTGCTTTCGAAGGGCTCGCCATTCGTCTTTCATTGCTGGTCAATTTGATGGAGTTACGAGATTTGAGGACTTTCCTAAAGTTGAACGTTTGCGAACATTCCTGCCACTATCGCTTTCAGATTCCAGGTGGTGGAGTAAATATTTGTCTCATAAGGTTATTTTTGAGTTACTACCACAGTTGCAATACTTACGAGTGCTCTCTTTGAATGGCTACAAAATAACTGAGCTGCCAAACTCAATTGGTGATTTGAGGTTGTTACAGTATCTTGACCTTTCCTACACAGATATAACCAGTTTGCCTGAATCAACAAGCACTCTTTACAGCTTGCAAACATTGATATTGGAAGGTTGTTCTAAATTGAAGTCATTGCCTGCGAAGATGAGTAATCTAATTAATTTGCGCCATCTAAACAACTTGGATGCATATTCGTTGGGAGGAATGCCTCCACAACTGGGTAGAATGGTGAATCTCCAATCATTGTCTAATTTTGTGGTCAGTGGTGGGAATGATGGATCAAGGATAAGGGAGATAGAGTTCCTATTGCATCTCCGCGGGACATTGTGCATCTCAAGGTTGGAGAATGTGATTGATGTCGAGGATGCTCGGAGGGCCAACTTAAAATGCAAGGAGAGGCTTGATTCATTGGTCCTAGAATGGTCTCATTCAAGCAACACAAGAGAAACGGAATCTGCTGTGCTTGACATGTTACAGCCTCATACAAAGCTCAAGGAGCTCACCATCAAGAGTTATGCCGGAAAGGAATTTTCATCATGGGTTGCAGTTTCTTTGTTCTCTAATATGGTGCTTGTGCGGTTAGAGGAGTGTAACAATTGTTTATCGTTGCCACCTCTCGGACAATTGCCTCATCTCAAAGAACTTTATATTAGAGGAATGAATGCAGTGGAAAGTGTTGGTGCTGAGTTTTATGGAGAGTGCATCTCGCCTTTTCCTCTATTAGAGACTCTTGAGTTTGTGGATATGCAACATTGGAAGGAATGGCTTCCTTTCCAATTGGATCGTGGAAATGGTGTTTTCCCTTGCCTGAAAACGCTCTTTGTAAAAAAATGTTCAAAACTGGGGGGTAAGTTGCCAGAGAACCTCAATTCTTTAGCAAAGCTTGGAATTGTTAAATGTGAAGAATTAGTGGTTTCAATTGCCAACTACAAGCAACTTTGTCATCTAAACATTGACTGTTGTAAAGTGTTGGTGCATACAGCTGCTAAGGTTGAGTTTGAGTTGTTAGAGTCCTTGTGCCTTTCAAACATTTCGGAGCTGATGTCTCTGCAAACAGGGGAATTGTTCCAGAAGGGATTAACCAAGGTTAGAGATTTGAAGATTAATGGATGTGAGGAGCTGATGTCTTCATTGAAGAACGAGGGTAGATTATTGCAACAGTTGACTTCTCTTGGCCGTTTGGAAATTGAAGACAACTCTCCTCTAGTTGAAGAATTGGGAAAAGAAGCAGAGGAGTTGCTGCAATTGCAAATATTGGAGTGCAAGCTTGAATGTCTGGAGTTAAAGAAGTGCGAAAATATTTTAAAGCTACCAAAAGGGTTAAATCAGTTGTTATCTCTTCAACAGCTTTGCATACACAAATGTCCACGTCTAGTTTCTTTTCCGGATGTCGGTCTGCCACCTTCTCTTGAAGACATCAAGATTACAGAGTGCCATTCGTTGATATATTTGGCAAAATTTCAGATTCCCCAAAATCTCAGAAGAATACAGATAAGTGACTGTAAAAGTTTGAAATCACTAGTAGATGAGGAGGTTGTTGGTTCTTCTATGTTGTCTTCTCACATTTGCCTTGAGTACTTGAAAATCCAGGAATGTCAATCTCTGACGTCGTTATCATTGAGCGGCCAGCTTCTTAGGACACTTAAACACCTTGAGATATACGATTGTTTACAGCTGAAGTTAATCGAAGAGGACGGGTTCTCCCACTACAACACTAATCAGTGTCTTGAATATATAAGGATCTGGAAGTGCCAAAATCTGAAATACTTACCAGATGGCTTATGCCACCTCATCAATCTTCAAACGTTGGATATTTATTACTGTGGAAGTCTTGTTTCTATCCCCAGACTAAGTGGGGGGAGAAAAGCCTCCAACCTGAGAGAGATCAAGATAACCGATTGCGACAAATTGGAGGTGTTACCCGAAGCCATGCGCAATCTCAACTCTCTTGAGGAATTGACCATCGACTACCGTGAAGGTTTGAGTTGCTTCTTTCCCGCCAACCTCACATCACTTACAATTTCGAAGGTCAAGAGCTGTAAGTCATTGTGGGAGTTGGAGTGGGGATTGCACAGACTCACCTCTCTTAGAAAATTATGGATCTACGGCAATGACCCGGATATGGTATCGTTTCCACCCGACGTGGTCCAGACGGAGACGCTCCTCCCCAAATCTCTCACTGGACTATTAATTTGTGGTTTCCCGAATCTGATGAAACTGAGTAGCAAGGGCTTTCAATCCCTAACGTCTCTTCAATCTCTGCATCTCTCCAGTTGTTTACAGCTAGCATCCATTCCAGAAGAGGGTCTTCCTCCTTCACTTACACAACTTCTCATCTCTAGGTGTCCTGTGCTAAAAGAGAGATGCGAACGAGGAAAAGGACGCTACTGGCACAAAATATCCCACATCCCTTACATAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1410

Amino Acids

160.86

Weight (kDa)

6.76

Isoelectric Point (pI)

51.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 99 4.5e-21 Rx N-terminal domain
NB-ARC PF00931 173 - 348 8.5e-37 NB-ARC domain
WHD_DRP PF23559 433 - 502 1.9e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 579 - 855 5.1e-21 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 698 - 822 1.2e-37 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 1123 - 1237 2.2e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 336
AarI CACCTGC 1 cut(s) 1580
AatII GACGTC 2 cut(s) 446, 3416
Acc16I TGCGCA 1 cut(s) 3740
Acc36I ACCTGC 2 cut(s) 554, 1580
AccB7I CCANNNNNTGG 1 cut(s) 1701
AccBSI CCGCTC 2 cut(s) 388, 3429
AccII CGCG 2 cut(s) 945, 2124
AccIII TCCGGA 1 cut(s) 3190
AciI CCGC 9 cut(s) 26, 388, 943, 975, 2122, 2124, 2383, 3429, 3804
AclI AACGTT 2 cut(s) 1657, 3611
AclWI GGATC 4 cut(s) 2092, 2595, 3554, 3914
AcoI YGGCCR 2 cut(s) 2980, 3430
AcsI RAATTY 5 cut(s) 254, 1267, 1316, 2333, 3263
AcuI CTGAAG 2 cut(s) 1398, 3503
AcyI GRCGYC 4 cut(s) 99, 443, 906, 3413
AfaI GTAC 3 cut(s) 568, 1236, 3383
AfiI CCNNNNNNNGG 9 cut(s) 171, 611, 612, 1701, 2024, 2423, 3197, 3928, 4109
AflIII ACRYGT 2 cut(s) 243, 2274
AhlI ACTAGT 1 cut(s) 3325
AjiI CACGTC 3 cut(s) 293, 3176, 3949
AjnI CCWGG 2 cut(s) 1694, 3393
AloI GAACNNNNNNTCC 2 cut(s) 2090, 2122
Alw21I GWGCWC 2 cut(s) 1770, 2307
Alw26I GTCTC 7 cut(s) 283, 1722, 2235, 2525, 2856, 3956, 4059
AlwI GGATC 4 cut(s) 2092, 2595, 3554, 3914
AlwNI CAGNNNCTG 2 cut(s) 43, 569
Aor13HI TCCGGA 1 cut(s) 3190
AoxI GGCC 5 cut(s) 1144, 1405, 2181, 2980, 3430
ApeKI GCWGC 7 cut(s) 43, 787, 1127, 1514, 1798, 2789, 3046
ApoI RAATTY 5 cut(s) 254, 1267, 1316, 2333, 3263
ArsI GACNNNNNNTTYG 2 cut(s) 3981, 4013
AseI ATTAAT 3 cut(s) 1965, 2910, 3995
Asp700I GAANNNNTTC 1 cut(s) 2571
AspLEI GCGC 3 cut(s) 279, 1974, 3741
AspS9I GGNCC 5 cut(s) 126, 1405, 2181, 2218, 3952
AsuC2I CCSGG 3 cut(s) 95, 606, 3923
AsuHPI GGTGA 7 cut(s) 166, 869, 1565, 1826, 2047, 2299, 3877
AsuII TTCGAA 3 cut(s) 1087, 1579, 3830
AsuNHI GCTAGC 1 cut(s) 4090
AvaII GGWCC 3 cut(s) 126, 2218, 3952
BanII GRGCYC 2 cut(s) 1588, 2307
BauI CACGAG 1 cut(s) 65
BbsI GAAGAC 8 cut(s) 246, 623, 1386, 2925, 3003, 3225, 3354, 4106
Bbv12I GWGCWC 2 cut(s) 1770, 2307
BbvI GCAGC 7 cut(s) 30, 799, 1114, 1526, 1785, 2776, 3033
BccI CCATC 9 cut(s) 1052, 1270, 1342, 1613, 1983, 2075, 2318, 3575, 3776
BceAI ACGGC 3 cut(s) 1392, 2967, 3928
BciT130I CCWGG 2 cut(s) 1696, 3395
BcnI CCSGG 3 cut(s) 95, 606, 3923
BcoDI GTCTC 7 cut(s) 283, 1722, 2235, 2525, 2856, 3956, 4059
BcuI ACTAGT 1 cut(s) 3325
BfaI CTAG 7 cut(s) 1533, 2222, 3011, 3179, 3326, 4091, 4146
BfmI CTRYAG 2 cut(s) 35, 591
BfuAI ACCTGC 2 cut(s) 554, 1580
BmcAI AGTACT 1 cut(s) 3383
Bme1390I CCNGG 5 cut(s) 95, 606, 1696, 3395, 3923
Bme18I GGWCC 3 cut(s) 126, 2218, 3952
BmgBI CACGTC 3 cut(s) 293, 3176, 3949
BmgT120I GGNCC 5 cut(s) 126, 1405, 2181, 2218, 3952
BmiI GGNNCC 1 cut(s) 973
BmrFI CCNGG 5 cut(s) 95, 606, 1696, 3395, 3923
BmrI ACTGGG 4 cut(s) 17, 447, 2033, 2657
BmtI GCTAGC 1 cut(s) 4094
BmuI ACTGGG 4 cut(s) 17, 447, 2033, 2657
BpiI GAAGAC 8 cut(s) 246, 623, 1386, 2925, 3003, 3225, 3354, 4106
BplI GAGNNNNNCTC 2 cut(s) 2495, 2527
BpmI CTGGAG 5 cut(s) 134, 936, 999, 3104, 4060
Bpu10I CCTNAGC 2 cut(s) 450, 2793
Bpu14I TTCGAA 3 cut(s) 1087, 1579, 3830
BpuEI CTTGAG 6 cut(s) 2125, 2282, 2558, 3398, 3479, 3776
BpuMI CCSGG 3 cut(s) 95, 606, 3923
BsaHI GRCGYC 4 cut(s) 99, 443, 906, 3413
BsaI GGTCTC 1 cut(s) 2235
BsaJI CCNNGG 4 cut(s) 94, 165, 2122, 2889
BsaWI WCCGGW 1 cut(s) 3190
Bsc4I CCNNNNNNNGG 9 cut(s) 171, 611, 612, 1701, 2024, 2423, 3197, 3928, 4109
Bse1I ACTGG 9 cut(s) 12, 442, 1067, 1863, 2028, 2652, 3991, 4077, 4199
Bse3DI GCAATG 3 cut(s) 1602, 1937, 3922
BseAI TCCGGA 1 cut(s) 3190
BseBI CCWGG 2 cut(s) 1696, 3395
BseDI CCNNGG 4 cut(s) 94, 165, 2122, 2889
BseGI GGATG 9 cut(s) 606, 749, 1353, 1996, 2176, 2921, 3199, 4094, 4212
BseLI CCNNNNNNNGG 9 cut(s) 171, 611, 612, 1701, 2024, 2423, 3197, 3928, 4109
BseMI GCAATG 3 cut(s) 1602, 1937, 3922
BseMII CTCAG 7 cut(s) 174, 229, 1785, 2481, 3298, 3674, 4016
BseNI ACTGG 9 cut(s) 12, 442, 1067, 1863, 2028, 2652, 3991, 4077, 4199
BseRI GAGGAG 7 cut(s) 785, 2405, 2936, 2997, 3053, 3350, 3959
BseXI GCAGC 7 cut(s) 30, 799, 1114, 1526, 1785, 2776, 3033
BsgI GTGCAG 1 cut(s) 495
Bsh1236I CGCG 2 cut(s) 945, 2124
BshFI GGCC 5 cut(s) 1146, 1407, 2183, 2982, 3432
BsiHKAI GWGCWC 2 cut(s) 1770, 2307
BsiSI CCGG 5 cut(s) 94, 606, 2325, 3191, 3923
BslFI GGGAC 2 cut(s) 454, 2140
BslI CCNNNNNNNGG 9 cut(s) 171, 611, 612, 1701, 2024, 2423, 3197, 3928, 4109
BsmAI GTCTC 7 cut(s) 283, 1722, 2235, 2525, 2856, 3956, 4059
BsmBI CGTCTC 3 cut(s) 283, 3956, 4059
BsmFI GGGAC 2 cut(s) 454, 2140
BsmI GAATGC 2 cut(s) 2016, 2473
BsnI GGCC 5 cut(s) 1146, 1407, 2183, 2982, 3432
Bso31I GGTCTC 1 cut(s) 2235
Bsp119I TTCGAA 3 cut(s) 1087, 1579, 3830
Bsp1286I GDGCHC 3 cut(s) 1588, 1770, 2307
Bsp13I TCCGGA 1 cut(s) 3190
Bsp143I GATC 9 cut(s) 211, 326, 865, 874, 2084, 2587, 3546, 3690, 3906
BspACI CCGC 9 cut(s) 26, 388, 943, 975, 2122, 2124, 2383, 3429, 3804
BspANI GGCC 5 cut(s) 1146, 1407, 2183, 2982, 3432
BspCNI CTCAG 7 cut(s) 175, 228, 1786, 2482, 3297, 3675, 4017
BspEI TCCGGA 1 cut(s) 3190
BspFNI CGCG 2 cut(s) 945, 2124
BspLI GGNNCC 1 cut(s) 973
BspMI ACCTGC 2 cut(s) 554, 1580
BspOI GCTAGC 1 cut(s) 4094
BspPI GGATC 4 cut(s) 2092, 2595, 3554, 3914
BspT104I TTCGAA 3 cut(s) 1087, 1579, 3830
BspTNI GGTCTC 1 cut(s) 2235
BsrBI CCGCTC 2 cut(s) 388, 3429
BsrDI GCAATG 3 cut(s) 1602, 1937, 3922
BsrI ACTGG 9 cut(s) 12, 442, 1067, 1863, 2028, 2652, 3991, 4077, 4199
BssECI CCNNGG 4 cut(s) 94, 165, 2122, 2889
BssMI GATC 9 cut(s) 211, 326, 865, 874, 2084, 2587, 3546, 3690, 3906
BssNI GRCGYC 4 cut(s) 99, 443, 906, 3413
BssSI CACGAG 1 cut(s) 65
BssT1I CCWWGG 1 cut(s) 2889
Bst2BI CACGAG 1 cut(s) 65
Bst2UI CCWGG 2 cut(s) 1696, 3395
Bst6I CTCTTC 6 cut(s) 502, 1268, 3150, 3489, 4062, 4101
BstACI GRCGYC 4 cut(s) 99, 443, 906, 3413
BstBI TTCGAA 3 cut(s) 1087, 1579, 3830
BstC8I GCNNGC 8 cut(s) 478, 561, 1588, 1898, 1944, 3071, 3434, 4092
BstDSI CCRYGG 1 cut(s) 2122
BstF5I GGATG 9 cut(s) 606, 749, 1353, 1996, 2176, 2921, 3199, 4094, 4212
BstFNI CGCG 2 cut(s) 945, 2124
BstHHI GCGC 3 cut(s) 279, 1974, 3741
BstKTI GATC 9 cut(s) 214, 329, 868, 877, 2087, 2590, 3549, 3693, 3909
BstMAI GTCTC 7 cut(s) 283, 1722, 2235, 2525, 2856, 3956, 4059
BstMBI GATC 9 cut(s) 211, 326, 865, 874, 2084, 2587, 3546, 3690, 3906
BstMWI GCNNNNNNNGC 5 cut(s) 1511, 1681, 2180, 2302, 3052
BstNI CCWGG 2 cut(s) 1696, 3395
BstNSI RCATGY 4 cut(s) 247, 563, 1040, 2278
BstSCI CCNGG 5 cut(s) 93, 604, 1694, 3393, 3921
BstSFI CTRYAG 2 cut(s) 35, 591
BstUI CGCG 2 cut(s) 945, 2124
BstV1I GCAGC 7 cut(s) 30, 799, 1114, 1526, 1785, 2776, 3033
BstV2I GAAGAC 8 cut(s) 246, 623, 1386, 2925, 3003, 3225, 3354, 4106
BstX2I RGATCY 2 cut(s) 3546, 3906
BstXI CCANNNNNNTGG 1 cut(s) 684
BstYI RGATCY 2 cut(s) 3546, 3906
BsuRI GGCC 5 cut(s) 1146, 1407, 2183, 2982, 3432
BtgI CCRYGG 1 cut(s) 2122
BtgZI GCGATG 1 cut(s) 960
BtrI CACGTC 3 cut(s) 293, 3176, 3949
BtsCI GGATG 9 cut(s) 606, 749, 1353, 1996, 2176, 2921, 3199, 4094, 4212
BtsI GCAGTG 1 cut(s) 2478
BtsIMutI CAGTG 5 cut(s) 5, 2074, 2478, 3533, 3984
BveI ACCTGC 2 cut(s) 554, 1580
Cac8I GCNNGC 8 cut(s) 478, 561, 1588, 1898, 1944, 3071, 3434, 4092
CaiI CAGNNNCTG 2 cut(s) 43, 569
CfoI GCGC 3 cut(s) 279, 1974, 3741
Cfr13I GGNCC 5 cut(s) 126, 1405, 2181, 2218, 3952
Cfr42I CCGCGG 1 cut(s) 2125
CseI GACGC 4 cut(s) 88, 895, 3973, 4197
Csp6I GTAC 3 cut(s) 567, 1235, 3382
CspCI CAANNNNNGTGG 2 cut(s) 129, 164
CviQI GTAC 3 cut(s) 567, 1235, 3382
DpnI GATC 9 cut(s) 213, 328, 867, 876, 2086, 2589, 3548, 3692, 3908
DpnII GATC 9 cut(s) 211, 326, 865, 874, 2084, 2587, 3546, 3690, 3906
DraI TTTAAA 1 cut(s) 3111
EaeI YGGCCR 2 cut(s) 2980, 3430
Eam1104I CTCTTC 6 cut(s) 502, 1268, 3150, 3489, 4062, 4101
EarI CTCTTC 6 cut(s) 502, 1268, 3150, 3489, 4062, 4101
Ecl136II GAGCTC 1 cut(s) 2305
Eco130I CCWWGG 1 cut(s) 2889
Eco24I GRGCYC 2 cut(s) 1588, 2307
Eco31I GGTCTC 1 cut(s) 2235
Eco32I GATATC 1 cut(s) 235
Eco47I GGWCC 3 cut(s) 126, 2218, 3952
Eco53kI GAGCTC 1 cut(s) 2305
Eco57I CTGAAG 2 cut(s) 1398, 3503
EcoICRI GAGCTC 1 cut(s) 2305
EcoRII CCWGG 2 cut(s) 1694, 3393
EcoRV GATATC 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 2889
EcoT22I ATGCAT 3 cut(s) 1042, 1485, 1996
EcoT38I GRGCYC 2 cut(s) 1588, 2307
ErhI CCWWGG 1 cut(s) 2889
Esp3I CGTCTC 3 cut(s) 283, 3956, 4059
FalI AAGNNNNNCTT 4 cut(s) 410, 442, 4098, 4130
FaqI GGGAC 2 cut(s) 454, 2140
FauI CCCGC 3 cut(s) 950, 2117, 3811
FokI GGATG 9 cut(s) 613, 736, 1360, 2003, 2183, 2928, 3206, 4081, 4199
FriOI GRGCYC 2 cut(s) 1588, 2307
FspBI CTAG 7 cut(s) 1533, 2222, 3011, 3179, 3326, 4091, 4146
FspI TGCGCA 1 cut(s) 3740
GlaI GCGC 3 cut(s) 278, 1973, 3740
GsuI CTGGAG 5 cut(s) 134, 936, 999, 3104, 4060
HaeIII GGCC 5 cut(s) 1146, 1407, 2183, 2982, 3432
HapII CCGG 5 cut(s) 94, 606, 2325, 3191, 3923
HgaI GACGC 4 cut(s) 88, 895, 3973, 4197
HhaI GCGC 3 cut(s) 279, 1974, 3741
Hin1I GRCGYC 4 cut(s) 99, 443, 906, 3413
Hin6I GCGC 3 cut(s) 277, 1972, 3739
HinP1I GCGC 3 cut(s) 277, 1972, 3739
HincII GTYRAC 2 cut(s) 57, 2970
HindII GTYRAC 2 cut(s) 57, 2970
HindIII AAGCTT 3 cut(s) 494, 2687, 3071
HpaII CCGG 5 cut(s) 94, 606, 2325, 3191, 3923
HphI GGTGA 7 cut(s) 166, 869, 1565, 1826, 2047, 2299, 3877
Hpy166II GTNNAC 7 cut(s) 57, 296, 467, 2970, 3173, 3476, 4085
Hpy8I GTNNAC 7 cut(s) 57, 296, 467, 2970, 3173, 3476, 4085
Hpy99I CGWCG 3 cut(s) 911, 3418, 3950
HpyF10VI GCNNNNNNNGC 5 cut(s) 1511, 1681, 2180, 2302, 3052
Hsp92I GRCGYC 4 cut(s) 99, 443, 906, 3413
HspAI GCGC 3 cut(s) 277, 1972, 3739
Kpn2I TCCGGA 1 cut(s) 3190
KspI CCGCGG 1 cut(s) 2125
Kzo9I GATC 9 cut(s) 211, 326, 865, 874, 2084, 2587, 3546, 3690, 3906
LmnI GCTCC 7 cut(s) 385, 911, 971, 2302, 2842, 2923, 3972
Lsp1109I GCAGC 7 cut(s) 30, 799, 1114, 1526, 1785, 2776, 3033
MaeI CTAG 7 cut(s) 1533, 2222, 3011, 3179, 3326, 4091, 4146
MalI GATC 9 cut(s) 213, 328, 867, 876, 2086, 2589, 3548, 3692, 3908
MbiI CCGCTC 2 cut(s) 388, 3429
MboI GATC 9 cut(s) 211, 326, 865, 874, 2084, 2587, 3546, 3690, 3906
MfeI CAATTG 6 cut(s) 1809, 2401, 2429, 2582, 2724, 3050
MflI RGATCY 2 cut(s) 3546, 3906
MhlI GDGCHC 3 cut(s) 1588, 1770, 2307
MlyI GAGTC 6 cut(s) 618, 990, 1129, 2527, 2825, 3876
MmeI TCCRAC 8 cut(s) 228, 484, 889, 1518, 2127, 3594, 3702, 3843
Mph1103I ATGCAT 3 cut(s) 1042, 1485, 1996
MroI TCCGGA 1 cut(s) 3190
MroXI GAANNNNTTC 1 cut(s) 2571
MslI CAYNNNNRTG 2 cut(s) 266, 651
MspA1I CMGCKG 4 cut(s) 977, 2124, 2789, 3481
MspI CCGG 5 cut(s) 94, 606, 2325, 3191, 3923
MspR9I CCNGG 5 cut(s) 95, 606, 1696, 3395, 3923
MunI CAATTG 6 cut(s) 1809, 2401, 2429, 2582, 2724, 3050
Mva1269I GAATGC 2 cut(s) 2016, 2473
MvaI CCWGG 2 cut(s) 1696, 3395
MvnI CGCG 2 cut(s) 945, 2124
MwoI GCNNNNNNNGC 5 cut(s) 1511, 1681, 2180, 2302, 3052
NciI CCSGG 3 cut(s) 95, 606, 3923
NdeII GATC 9 cut(s) 211, 326, 865, 874, 2084, 2587, 3546, 3690, 3906
NheI GCTAGC 1 cut(s) 4090
NlaIV GGNNCC 1 cut(s) 973
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 6 cut(s) 721, 857, 934, 1220, 1409, 3302
NsbI TGCGCA 1 cut(s) 3740
NsiI ATGCAT 3 cut(s) 1042, 1485, 1996
NspI RCATGY 4 cut(s) 247, 563, 1040, 2278
NspV TTCGAA 3 cut(s) 1087, 1579, 3830
PaeI GCATGC 1 cut(s) 563
PaqCI CACCTGC 1 cut(s) 1580
PciI ACATGT 2 cut(s) 243, 2274
PctI GAATGC 2 cut(s) 2016, 2473
PdmI GAANNNNTTC 1 cut(s) 2571
PflFI GACNNNGTC 1 cut(s) 3950
PflMI CCANNNNNTGG 1 cut(s) 1701
PfoI TCCNGGA 1 cut(s) 3393
PleI GAGTC 6 cut(s) 617, 989, 1129, 2527, 2824, 3876
PpsI GAGTC 6 cut(s) 617, 989, 1129, 2527, 2824, 3876
PscI ACATGT 2 cut(s) 243, 2274
PshBI ATTAAT 3 cut(s) 1965, 2910, 3995
PsiI TTATAA 1 cut(s) 336
Psp124BI GAGCTC 1 cut(s) 2307
Psp1406I AACGTT 2 cut(s) 1657, 3611
Psp6I CCWGG 2 cut(s) 1694, 3393
PspGI CCWGG 2 cut(s) 1694, 3393
PspN4I GGNNCC 1 cut(s) 973
PspPI GGNCC 5 cut(s) 126, 1405, 2181, 2218, 3952
PstNI CAGNNNCTG 2 cut(s) 43, 569
PsuI RGATCY 2 cut(s) 3546, 3906
PsyI GACNNNGTC 1 cut(s) 3950
PvuII CAGCTG 2 cut(s) 2789, 3481
RsaI GTAC 3 cut(s) 568, 1236, 3383
RsaNI GTAC 3 cut(s) 567, 1235, 3382
RseI CAYNNNNRTG 2 cut(s) 266, 651
SacI GAGCTC 1 cut(s) 2307
SacII CCGCGG 1 cut(s) 2125
Sau3AI GATC 9 cut(s) 211, 326, 865, 874, 2084, 2587, 3546, 3690, 3906
Sau96I GGNCC 5 cut(s) 126, 1405, 2181, 2218, 3952
ScaI AGTACT 1 cut(s) 3383
SchI GAGTC 6 cut(s) 618, 990, 1129, 2527, 2825, 3876
ScrFI CCNGG 5 cut(s) 95, 606, 1696, 3395, 3923
SduI GDGCHC 3 cut(s) 1588, 1770, 2307
SfcI CTRYAG 2 cut(s) 35, 591
Sfr303I CCGCGG 1 cut(s) 2125
SfuI TTCGAA 3 cut(s) 1087, 1579, 3830
SgrBI CCGCGG 1 cut(s) 2125
SinI GGWCC 3 cut(s) 126, 2218, 3952
SmiMI CAYNNNNRTG 2 cut(s) 266, 651
SmlI CTYRAG 6 cut(s) 2140, 2297, 2537, 3377, 3458, 3755
SmoI CTYRAG 6 cut(s) 2140, 2297, 2537, 3377, 3458, 3755
SpeI ACTAGT 1 cut(s) 3325
SphI GCATGC 1 cut(s) 563
SsiI CCGC 9 cut(s) 26, 388, 943, 975, 2122, 2124, 2383, 3429, 3804
SspI AATATT 5 cut(s) 1026, 1185, 1712, 3060, 3106
SspMI CTAG 7 cut(s) 1533, 2222, 3011, 3179, 3326, 4091, 4146
SstI GAGCTC 1 cut(s) 2307
StyD4I CCNGG 5 cut(s) 93, 604, 1694, 3393, 3921
StyI CCWWGG 1 cut(s) 2889
TaqII GACCGA 1 cut(s) 3188
TatI WGTACW 1 cut(s) 3381
TauI GCSGC 3 cut(s) 29, 977, 3432
TscAI CASTG 5 cut(s) 12, 2074, 2478, 3533, 3991
TseFI GTSAC 6 cut(s) 721, 857, 934, 1220, 1409, 3302
TseI GCWGC 7 cut(s) 43, 787, 1127, 1514, 1798, 2789, 3046
Tsp45I GTSAC 6 cut(s) 721, 857, 934, 1220, 1409, 3302
TspGWI ACGGA 4 cut(s) 337, 424, 2270, 3974
TspRI CASTG 5 cut(s) 12, 2074, 2478, 3533, 3991
Tth111I GACNNNGTC 1 cut(s) 3950
Van91I CCANNNNNTGG 1 cut(s) 1701
VpaK11BI GGWCC 3 cut(s) 126, 2218, 3952
VspI ATTAAT 3 cut(s) 1965, 2910, 3995
XapI RAATTY 5 cut(s) 254, 1267, 1316, 2333, 3263
XceI RCATGY 4 cut(s) 247, 563, 1040, 2278
XcmI CCANNNNNNNNNTGG 1 cut(s) 2588
XmnI GAANNNNTTC 1 cut(s) 2571
XspI CTAG 7 cut(s) 1533, 2222, 3011, 3179, 3326, 4091, 4146
ZraI GACGTC 2 cut(s) 444, 3414
ZrmI AGTACT 1 cut(s) 3383
Zsp2I ATGCAT 3 cut(s) 1042, 1485, 1996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.