RchiOBHm_Chr1g0320551

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000070
Physical Location & Seq
Reverse (-)
8190758 .. 8193722
2965 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55069

Sequence Viewer

Length: 2883 bp
ATGGCAGTGGGAGAGATCTTTCTTGTGGCATTCCTTCAGTTGCTGCTGGACAGGTTAACGCCTCGGGAGATCCTGGGCAACTTTGGAAGCTTGGGAGGAGTTGGCAAAAAGCTGCAGAAATGGAGGGAAACACTGTCGGCAGTTGCAGCTTTGCTTTCCGATGCGGAGGAGAAGCAGCTGACGAGCAAGGCAGTGGATCTGTGGCTGAATGACCTCAAACACTTGGCTTACGATATGGACGATCTACTCAACACCTTTTCTAATGAGATGTTAGAACGAAGCAAGGTTGACCAAACTGGCACAAGCAAGGTACGAGGCTTCTTTACCAAAGTTCCTCACAAAGTTAAGTTCAATTTCAATATGAACTCCGAAATAGACGACATTGCTAATCGCTTGCAAGACATATTTGACCGGAAAGAGAAACTCGGTTTGAAATATATTGAGCATACCTCTACTTCAACATCGTCATCGCACAGGACACCAAGTTCATATGTGTTGGATGGACCTGTGGTTGGGAGAGATGAAGACACAAGAAAGATTGTGGAATTGTTGTCCAGAAATGCTGATCCTTCTTCTCCCACAAATTATCAAGTTGTTGCCATTGTTGGTATGGGTGGACTCGGCAAGACAACGCTTGCGGGACAAGTCTTCAATGATGTAGTTGCAATGAAACAGTTTAGTCTCAAGATCTGGGTTTCAGTGTCCGATGACTTCGATCTTCAAACGGTGACAAGAACTATTTTTAAGGAAGTCACATCTCGGCCATGTGATATGGATGAGCTCAGTCGACTTCAAGATAATCTGAGCAAGGAGATTGATGGTAAAAGGTTTTTGATTGTTTTAGATGATGTATGGAGCACGTGTGATTATGATTCATGGACAAAACTGCAAGCTCCCCTCCGTGGCGGAGCCAAGGGAAGTAAGGTAATGGTGACAACACGTGATGAAAAAGTAGCTGCATTGACGGGAGCCCCAGCCGCTGAAGTTTATTATTTAAAGACTCTATCAGATGAAGGTTGTTTGCAAGTATTTGAGCAGCATGTTGGCAATGACAGGCCACCGAATTTCGAGTTGCTTAAGAAGAAAATTATTACGAACTGCAATGGATTGCCATTGGCTGCAAAAACTCTTGGTGGTGTTTTACGTTGTAAAGAAACTGACAAGTGGGAGGAAATACTAAATGACAAATTATGGAGTATATCAGATGGGAGAAAAATACTCCCTGTACTGAGATTGAGTTATCATTATCTCCCTTCAACTTTGAAGAGGTGCTTTGTCTATTGCTCGATACTTCCCAATGACTATGAATTTGGGAAAACCCAATTGATCCTTCTGTGGATGGCAGAGGGTTTCCTTGAGCAACCAGAAGGGAAAGCAATGGAAGATATTGGTGACGAATATTTTGGGGAGCTATTGTCTCGGTCATTATTTCAGAACTCGGGCAAAAACAGTTTGTGCTATGTAATGCACGACCTTGTTGGTGATTTGGCACGATGGGCTGCTGGAGACACATTTTGCAGATTGGAGGATAAACGGCATGGTAGATGTTCTGCAAATACTCGTCATCTAACTTACGTTTCTGGTAAGTTCGATGGGGCAAAAAGATTTGAGACATTTTCTGAAGCAAAACGCTTGCGGACTTTCCTACCACTTCCTGTTTCCGGTGGTTATGAGAATTATGTAACTCGTTATGCTACTTTTAATTTATTGCCACAATTGAAGTACTTGCGGGTGCTCTCCTTCAATGGCTATAAACTGACCGAGGTGCCAAATTCCGTAGGTAAGTTGAGACATCTACAATACCTTGATCTTTCTCACACATTAATAATGAGTTTGCCTGAGTCAACATGCATGCTTTACAACTTACAGACGTTAATATTAGAGAATTGTTCAAAATTGAAGACACTACCTTCAAACATGAGCAATTTATCTAATTTACGTCATCTCAACAATTCAAACATGCCTTCATTGGAAGAAATGCCTCCCAAAGTAAGTCGGTTGACTCATCTGCGAACTTTGCCTAATTTTGTGGTGGGGAAAGGTAGTGCATCAGGGATTGGAGAGATACAGTCATTGCATCTTCGTGGGACCTTGAGTGTTGGAAGATTGGAAAATGTGATTGATGTTGGGGATGCGAAGACGGCTCAAATAATGAACAAGGAAGGGCTGGAAATCTTGCAATTGGAATGGAGCGGCACAAGCGAGAAGGAATTGGAGGTGCTGTGCAGTTTAGAACCTCATAAAAAGCTTAAGGAGCTGACCATCAAGGGCTACAATGGTTTCGAATTTTCAAAATGGATTGGACATCCTTCATTCTCTGATATGATATCTATGAAGTTAGAGAACTGTAAAAACTGTCGATTTTTACCTCCTCTTGGCCAATTACCTTTTCTGAAAAAACTTCAGATACAAGGACTGGTCAATGTTAAAAGTGTGGGTGCTGAGTTTGATGGAGAGTGTAGCTTGCCTTTTCCAGTGTTGGAAGCCCTGACGTTCAAGGATATGCAAAATTGGAAGGAGTGGCTTCCTTGCAAAAGAGATGAAGAAATTCGATTTTTCCCATGCATGGAAAAGCTTTCCATCCTTAGTTGCCCCAAATTGAAAGGTTGGTTGCCCAAGAACATGGATTCATTATCAGAGCTGTATATTGATGAATGTGAAGAGTTAGTGGTTTCAATTGCCCATTATAAACATATACATAAGTTGACCATCAACGGTTGTAAAAGGGTGGTGCACAGAAGTGGAGTTAAGTTTGAGCTATTGGAGTATATGGGGCTTTCAAGTATACCAGAGTTCAGACTCGAAATAGACGGAGATGTCGGATTTCTAATATTATTGGAGGCAACATCTCTGACTCCGCCTCTCACTTTATTCAAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

960

Amino Acids

108.44

Weight (kDa)

7.03

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 98 3.1e-18 Rx N-terminal domain
NB-ARC PF00931 175 - 350 2.4e-36 NB-ARC domain
WHD_DRP PF23559 430 - 497 4.2e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 570 - 835 8.9e-22 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 686 - 805 1e-33 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2688
AasI GACNNNNNNGTC 1 cut(s) 2816
AccB1I GGYRCC 1 cut(s) 1765
AccBSI CCGCTC 1 cut(s) 2193
AccI GTMKAC 2 cut(s) 787, 2785
AciI CCGC 8 cut(s) 164, 638, 906, 978, 1636, 1729, 2193, 2858
AclWI GGATC 4 cut(s) 64, 204, 560, 1321
AcoI YGGCCR 2 cut(s) 761, 2377
AcsI RAATTY 5 cut(s) 1063, 1307, 1771, 2285, 2547
AcuI CTGAAG 4 cut(s) 20, 1002, 1641, 2387
AcvI CACGTG 2 cut(s) 861, 941
AfaI GTAC 3 cut(s) 312, 1227, 1724
AfiI CCNNNNNNNGG 5 cut(s) 512, 902, 1661, 2375, 2566
AflII CTTAAG 2 cut(s) 1076, 2249
AflIII ACRYGT 2 cut(s) 860, 938
AjnI CCWGG 1 cut(s) 72
AjuI GAANNNNNNNTTGG 4 cut(s) 1314, 1346, 2373, 2405
AleI CACNNNNGTG 1 cut(s) 2739
AloI GAACNNNNNNTCC 2 cut(s) 469, 501
Alw21I GWGCWC 4 cut(s) 783, 860, 1737, 2736
Alw26I GTCTC 5 cut(s) 686, 1422, 1500, 1604, 1783
Alw44I GTGCAC 1 cut(s) 2732
AlwI GGATC 4 cut(s) 64, 204, 560, 1321
AlwNI CAGNNNCTG 2 cut(s) 43, 980
Ama87I CYCGRG 2 cut(s) 63, 1438
AoxI GGCC 3 cut(s) 761, 1055, 2377
ApaLI GTGCAC 1 cut(s) 2732
ApeKI GCWGC 8 cut(s) 43, 112, 146, 175, 956, 1036, 1118, 1499
ApoI RAATTY 5 cut(s) 1063, 1307, 1771, 2285, 2547
ArsI GACNNNNNNTTYG 4 cut(s) 1292, 1324, 1385, 1417
AseI ATTAAT 1 cut(s) 1823
Asp700I GAANNNNTTC 1 cut(s) 2547
AspS9I GGNCC 2 cut(s) 503, 2088
AsuHPI GGTGA 4 cut(s) 739, 943, 1403, 1493
AsuII TTCGAA 1 cut(s) 2283
AvaI CYCGRG 2 cut(s) 63, 1438
AvaII GGWCC 2 cut(s) 503, 2088
BaeGI GKGCMC 1 cut(s) 2736
BalI TGGCCA 1 cut(s) 2379
BanI GGYRCC 1 cut(s) 1765
BanII GRGCYC 2 cut(s) 783, 973
BbrPI CACGTG 2 cut(s) 861, 941
BbsI GAAGAC 4 cut(s) 531, 640, 1907, 2144
Bbv12I GWGCWC 4 cut(s) 783, 860, 1737, 2736
BbvI GCAGC 8 cut(s) 30, 99, 158, 187, 943, 1048, 1105, 1486
BceAI ACGGC 2 cut(s) 1550, 2157
BciT130I CCWGG 1 cut(s) 74
BcoDI GTCTC 5 cut(s) 686, 1422, 1500, 1604, 1783
BfmI CTRYAG 1 cut(s) 113
BfrI CTTAAG 2 cut(s) 1076, 2249
BglII AGATCT 2 cut(s) 15, 687
BmcAI AGTACT 1 cut(s) 1724
Bme1390I CCNGG 1 cut(s) 74
Bme18I GGWCC 2 cut(s) 503, 2088
BmeT110I CYCGRG 2 cut(s) 63, 1438
BmgT120I GGNCC 2 cut(s) 503, 2088
BmiI GGNNCC 4 cut(s) 910, 970, 1767, 2089
BmrFI CCNGG 1 cut(s) 74
BmsI GCATC 4 cut(s) 151, 2057, 2086, 2122
BpiI GAAGAC 4 cut(s) 531, 640, 1907, 2144
BplI GAGNNNNNCTC 2 cut(s) 434, 466
BpmI CTGGAG 1 cut(s) 1524
Bpu14I TTCGAA 1 cut(s) 2283
BpuEI CTTGAG 3 cut(s) 668, 1376, 2113
BsaAI YACGTR 2 cut(s) 861, 941
BsaJI CCNNGG 5 cut(s) 62, 73, 901, 912, 1761
BsaWI WCCGGW 2 cut(s) 411, 1661
BsaXI ACNNNNNCTCC 6 cut(s) 847, 877, 1400, 1430, 2207, 2237
Bsc4I CCNNNNNNNGG 5 cut(s) 512, 902, 1661, 2375, 2566
Bse1I ACTGG 3 cut(s) 301, 2421, 2474
Bse3DI GCAATG 6 cut(s) 381, 672, 1054, 1108, 1383, 2072
BseBI CCWGG 1 cut(s) 74
BseDI CCNNGG 5 cut(s) 62, 73, 901, 912, 1761
BseGI GGATG 6 cut(s) 505, 781, 1344, 2137, 2305, 2580
BseLI CCNNNNNNNGG 5 cut(s) 512, 902, 1661, 2375, 2566
BseMI GCAATG 6 cut(s) 381, 672, 1054, 1108, 1383, 2072
BseMII CTCAG 5 cut(s) 794, 796, 1220, 1830, 2433
BseNI ACTGG 3 cut(s) 301, 2421, 2474
BseRI GAGGAG 3 cut(s) 111, 182, 2361
BseSI GKGCMC 1 cut(s) 2736
BseXI GCAGC 8 cut(s) 30, 99, 158, 187, 943, 1048, 1105, 1486
BseYI CCCAGC 1 cut(s) 973
BsgI GTGCAG 1 cut(s) 2245
BshFI GGCC 3 cut(s) 763, 1057, 2379
BshNI GGYRCC 1 cut(s) 1765
BsiHKAI GWGCWC 4 cut(s) 783, 860, 1737, 2736
BsiHKCI CYCGRG 2 cut(s) 63, 1438
BsiSI CCGG 2 cut(s) 412, 1662
BslFI GGGAC 2 cut(s) 654, 2101
BslI CCNNNNNNNGG 5 cut(s) 512, 902, 1661, 2375, 2566
BsmAI GTCTC 5 cut(s) 686, 1422, 1500, 1604, 1783
BsmFI GGGAC 2 cut(s) 654, 2101
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 3 cut(s) 763, 1057, 2379
BsoBI CYCGRG 2 cut(s) 63, 1438
Bsp119I TTCGAA 1 cut(s) 2283
Bsp1286I GDGCHC 5 cut(s) 783, 860, 973, 1737, 2736
Bsp143I GATC 9 cut(s) 15, 69, 196, 241, 565, 687, 715, 1326, 1807
BspACI CCGC 8 cut(s) 164, 638, 906, 978, 1636, 1729, 2193, 2858
BspANI GGCC 3 cut(s) 763, 1057, 2379
BspCNI CTCAG 5 cut(s) 795, 795, 1221, 1831, 2434
BspLI GGNNCC 4 cut(s) 910, 970, 1767, 2089
BspMAI CTGCAG 1 cut(s) 117
BspPI GGATC 4 cut(s) 64, 204, 560, 1321
BspT104I TTCGAA 1 cut(s) 2283
BspT107I GGYRCC 1 cut(s) 1765
BspTI CTTAAG 2 cut(s) 1076, 2249
BsrBI CCGCTC 1 cut(s) 2193
BsrDI GCAATG 6 cut(s) 381, 672, 1054, 1108, 1383, 2072
BsrI ACTGG 3 cut(s) 301, 2421, 2474
BssECI CCNNGG 5 cut(s) 62, 73, 901, 912, 1761
BssMI GATC 9 cut(s) 15, 69, 196, 241, 565, 687, 715, 1326, 1807
BssNAI GTATAC 1 cut(s) 2786
BssT1I CCWWGG 1 cut(s) 912
Bst1107I GTATAC 1 cut(s) 2786
Bst2UI CCWGG 1 cut(s) 74
Bst4CI ACNGT 8 cut(s) 135, 675, 727, 1451, 2070, 2348, 2357, 2717
Bst6I CTCTTC 2 cut(s) 1259, 2655
BstAFI CTTAAG 2 cut(s) 1076, 2249
BstBAI YACGTR 2 cut(s) 861, 941
BstBI TTCGAA 1 cut(s) 2283
BstC8I GCNNGC 6 cut(s) 395, 636, 891, 1634, 1853, 2465
BstDEI CTNAG 6 cut(s) 782, 803, 1229, 1839, 2442, 2585
BstDSI CCRYGG 1 cut(s) 901
BstF5I GGATG 6 cut(s) 505, 781, 1344, 2137, 2305, 2580
BstKTI GATC 9 cut(s) 18, 72, 199, 244, 568, 690, 718, 1329, 1810
BstMAI GTCTC 5 cut(s) 686, 1422, 1500, 1604, 1783
BstMBI GATC 9 cut(s) 15, 69, 196, 241, 565, 687, 715, 1326, 1807
BstMWI GCNNNNNNNGC 7 cut(s) 146, 977, 1496, 2017, 2141, 2199, 2254
BstNI CCWGG 1 cut(s) 74
BstNSI RCATGY 4 cut(s) 1043, 1851, 1855, 1963
BstSCI CCNGG 1 cut(s) 72
BstSFI CTRYAG 1 cut(s) 113
BstSLI GKGCMC 1 cut(s) 2736
BstV1I GCAGC 8 cut(s) 30, 99, 158, 187, 943, 1048, 1105, 1486
BstV2I GAAGAC 4 cut(s) 531, 640, 1907, 2144
BstX2I RGATCY 4 cut(s) 15, 69, 196, 687
BstXI CCANNNNNNTGG 1 cut(s) 2623
BstYI RGATCY 4 cut(s) 15, 69, 196, 687
BstZ17I GTATAC 1 cut(s) 2786
BsuRI GGCC 3 cut(s) 763, 1057, 2379
BtgI CCRYGG 1 cut(s) 901
BtgZI GCGATG 1 cut(s) 453
BtsCI GGATG 6 cut(s) 505, 781, 1344, 2137, 2305, 2580
BtsI GCAGTG 2 cut(s) 12, 198
BtsIMutI CAGTG 5 cut(s) 12, 131, 198, 705, 2481
Cac8I GCNNGC 6 cut(s) 395, 636, 891, 1634, 1853, 2465
CaiI CAGNNNCTG 2 cut(s) 43, 980
Cfr13I GGNCC 2 cut(s) 503, 2088
Csp6I GTAC 3 cut(s) 311, 1226, 1723
CviQI GTAC 3 cut(s) 311, 1226, 1723
DdeI CTNAG 6 cut(s) 782, 803, 1229, 1839, 2442, 2585
DpnI GATC 9 cut(s) 17, 71, 198, 243, 567, 689, 717, 1328, 1809
DpnII GATC 9 cut(s) 15, 69, 196, 241, 565, 687, 715, 1326, 1807
DraI TTTAAA 1 cut(s) 996
DrdI GACNNNNNNGTC 1 cut(s) 2816
DseDI GACNNNNNNGTC 1 cut(s) 2816
EaeI YGGCCR 2 cut(s) 761, 2377
Eam1104I CTCTTC 2 cut(s) 1259, 2655
EarI CTCTTC 2 cut(s) 1259, 2655
EciI GGCGGA 2 cut(s) 921, 2847
Ecl136II GAGCTC 1 cut(s) 781
Eco130I CCWWGG 1 cut(s) 912
Eco24I GRGCYC 2 cut(s) 783, 973
Eco32I GATATC 1 cut(s) 2328
Eco47I GGWCC 2 cut(s) 503, 2088
Eco53kI GAGCTC 1 cut(s) 781
Eco57I CTGAAG 4 cut(s) 20, 1002, 1641, 2387
Eco72I CACGTG 2 cut(s) 861, 941
Eco88I CYCGRG 2 cut(s) 63, 1438
EcoICRI GAGCTC 1 cut(s) 781
EcoO109I RGGNCCY 1 cut(s) 2088
EcoRII CCWGG 1 cut(s) 72
EcoRV GATATC 1 cut(s) 2328
EcoT14I CCWWGG 1 cut(s) 912
EcoT22I ATGCAT 2 cut(s) 1853, 2567
EcoT38I GRGCYC 2 cut(s) 783, 973
ErhI CCWWGG 1 cut(s) 912
FalI AAGNNNNNCTT 2 cut(s) 1256, 1288
FaqI GGGAC 2 cut(s) 654, 2101
FauI CCCGC 2 cut(s) 631, 1722
FauNDI CATATG 1 cut(s) 490
FblI GTMKAC 2 cut(s) 787, 2785
FokI GGATG 6 cut(s) 512, 788, 1351, 2144, 2292, 2567
FriOI GRGCYC 2 cut(s) 783, 973
GsaI CCCAGC 1 cut(s) 977
GsuI CTGGAG 1 cut(s) 1524
HaeIII GGCC 3 cut(s) 763, 1057, 2379
HapII CCGG 2 cut(s) 412, 1662
HincII GTYRAC 6 cut(s) 57, 289, 788, 1845, 2001, 2706
HindII GTYRAC 6 cut(s) 57, 289, 788, 1845, 2001, 2706
HindIII AAGCTT 3 cut(s) 88, 2246, 2573
HinfI GANTC 8 cut(s) 618, 872, 1000, 1841, 2002, 2627, 2799, 2854
HpaI GTTAAC 1 cut(s) 57
HpaII CCGG 2 cut(s) 412, 1662
HphI GGTGA 4 cut(s) 739, 943, 1403, 1493
Hpy166II GTNNAC 9 cut(s) 57, 289, 617, 788, 1845, 2001, 2706, 2734, 2786
Hpy188III TCNNGA 4 cut(s) 65, 555, 685, 794
Hpy8I GTNNAC 9 cut(s) 57, 289, 617, 788, 1845, 2001, 2706, 2734, 2786
HpyCH4III ACNGT 8 cut(s) 135, 675, 727, 1451, 2070, 2348, 2357, 2717
HpyCH4IV ACGT 7 cut(s) 860, 940, 1144, 1575, 1871, 1939, 2492
HpyF10VI GCNNNNNNNGC 7 cut(s) 146, 977, 1496, 2017, 2141, 2199, 2254
HpyF3I CTNAG 6 cut(s) 782, 803, 1229, 1839, 2442, 2585
HpySE526I ACGT 7 cut(s) 860, 940, 1144, 1575, 1871, 1939, 2492
KspAI GTTAAC 1 cut(s) 57
Kzo9I GATC 9 cut(s) 15, 69, 196, 241, 565, 687, 715, 1326, 1807
LmnI GCTCC 7 cut(s) 855, 898, 908, 968, 1408, 2190, 2254
Lsp1109I GCAGC 8 cut(s) 30, 99, 158, 187, 943, 1048, 1105, 1486
LweI GCATC 4 cut(s) 151, 2057, 2086, 2122
MaeII ACGT 7 cut(s) 860, 940, 1144, 1575, 1871, 1939, 2492
MaeIII GTNAC 5 cut(s) 727, 751, 931, 1391, 1681
MalI GATC 9 cut(s) 17, 71, 198, 243, 567, 689, 717, 1328, 1809
MbiI CCGCTC 1 cut(s) 2193
MboI GATC 9 cut(s) 15, 69, 196, 241, 565, 687, 715, 1326, 1807
MfeI CAATTG 4 cut(s) 1322, 1715, 2180, 2676
MflI RGATCY 4 cut(s) 15, 69, 196, 687
MhlI GDGCHC 5 cut(s) 783, 860, 973, 1737, 2736
MlsI TGGCCA 1 cut(s) 2379
MluNI TGGCCA 1 cut(s) 2379
MlyI GAGTC 6 cut(s) 612, 994, 1850, 1996, 2793, 2848
MmeI TCCRAC 4 cut(s) 477, 2080, 2460, 2800
Mox20I TGGCCA 1 cut(s) 2379
Mph1103I ATGCAT 2 cut(s) 1853, 2567
MroXI GAANNNNTTC 1 cut(s) 2547
MscI TGGCCA 1 cut(s) 2379
MslI CAYNNNNRTG 2 cut(s) 2082, 2739
Msp20I TGGCCA 1 cut(s) 2379
MspA1I CMGCKG 2 cut(s) 178, 980
MspCI CTTAAG 2 cut(s) 1076, 2249
MspI CCGG 2 cut(s) 412, 1662
MspR9I CCNGG 1 cut(s) 74
MunI CAATTG 4 cut(s) 1322, 1715, 2180, 2676
Mva1269I GAATGC 1 cut(s) 29
MvaI CCWGG 1 cut(s) 74
MwoI GCNNNNNNNGC 7 cut(s) 146, 977, 1496, 2017, 2141, 2199, 2254
NdeI CATATG 1 cut(s) 490
NdeII GATC 9 cut(s) 15, 69, 196, 241, 565, 687, 715, 1326, 1807
NlaIV GGNNCC 4 cut(s) 910, 970, 1767, 2089
NmeAIII GCCGAG 2 cut(s) 600, 739
NmuCI GTSAC 4 cut(s) 727, 751, 931, 1391
NsiI ATGCAT 2 cut(s) 1853, 2567
NspI RCATGY 4 cut(s) 1043, 1851, 1855, 1963
NspV TTCGAA 1 cut(s) 2283
OliI CACNNNNGTG 1 cut(s) 2739
PaeI GCATGC 1 cut(s) 1855
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PctI GAATGC 1 cut(s) 29
PdmI GAANNNNTTC 1 cut(s) 2547
PfeI GAWTC 2 cut(s) 872, 2627
PleI GAGTC 6 cut(s) 612, 994, 1849, 1996, 2793, 2848
PmaCI CACGTG 2 cut(s) 861, 941
PmlI CACGTG 2 cut(s) 861, 941
PpsI GAGTC 6 cut(s) 612, 994, 1849, 1996, 2793, 2848
Ppu21I YACGTR 2 cut(s) 861, 941
PpuMI RGGWCCY 1 cut(s) 2088
PshBI ATTAAT 1 cut(s) 1823
PsiI TTATAA 1 cut(s) 2688
Psp124BI GAGCTC 1 cut(s) 783
Psp5II RGGWCCY 1 cut(s) 2088
Psp6I CCWGG 1 cut(s) 72
PspCI CACGTG 2 cut(s) 861, 941
PspFI CCCAGC 1 cut(s) 973
PspGI CCWGG 1 cut(s) 72
PspN4I GGNNCC 4 cut(s) 910, 970, 1767, 2089
PspPI GGNCC 2 cut(s) 503, 2088
PspPPI RGGWCCY 1 cut(s) 2088
PstI CTGCAG 1 cut(s) 117
PstNI CAGNNNCTG 2 cut(s) 43, 980
PsuI RGATCY 4 cut(s) 15, 69, 196, 687
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 3 cut(s) 312, 1227, 1724
RsaNI GTAC 3 cut(s) 311, 1226, 1723
RseI CAYNNNNRTG 2 cut(s) 2082, 2739
SacI GAGCTC 1 cut(s) 783
SalI GTCGAC 1 cut(s) 786
Sau3AI GATC 9 cut(s) 15, 69, 196, 241, 565, 687, 715, 1326, 1807
Sau96I GGNCC 2 cut(s) 503, 2088
ScaI AGTACT 1 cut(s) 1724
SchI GAGTC 6 cut(s) 612, 994, 1850, 1996, 2793, 2848
ScrFI CCNGG 1 cut(s) 74
SduI GDGCHC 5 cut(s) 783, 860, 973, 1737, 2736
SfaNI GCATC 4 cut(s) 151, 2057, 2086, 2122
SfcI CTRYAG 1 cut(s) 113
SfuI TTCGAA 1 cut(s) 2283
SinI GGWCC 2 cut(s) 503, 2088
SmiMI CAYNNNNRTG 2 cut(s) 2082, 2739
SmlI CTYRAG 5 cut(s) 683, 1076, 1355, 2092, 2249
SmoI CTYRAG 5 cut(s) 683, 1076, 1355, 2092, 2249
SphI GCATGC 1 cut(s) 1855
SsiI CCGC 8 cut(s) 164, 638, 906, 978, 1636, 1729, 2193, 2858
SspI AATATT 3 cut(s) 1400, 1878, 2832
SstI GAGCTC 1 cut(s) 783
StyD4I CCNGG 1 cut(s) 72
StyI CCWWGG 1 cut(s) 912
TaaI ACNGT 8 cut(s) 135, 675, 727, 1451, 2070, 2348, 2357, 2717
TaiI ACGT 7 cut(s) 863, 943, 1147, 1578, 1874, 1942, 2495
TaqI TCGA 9 cut(s) 714, 787, 1068, 1286, 1590, 2283, 2359, 2551, 2802
TaqII GACCGA 2 cut(s) 1410, 1775
TatI WGTACW 2 cut(s) 1225, 1722
TauI GCSGC 2 cut(s) 980, 2196
TfiI GAWTC 2 cut(s) 872, 2627
TscAI CASTG 5 cut(s) 12, 138, 198, 705, 2481
TseFI GTSAC 4 cut(s) 727, 751, 931, 1391
TseI GCWGC 8 cut(s) 43, 112, 146, 175, 956, 1036, 1118, 1499
Tsp45I GTSAC 4 cut(s) 727, 751, 931, 1391
TspGWI ACGGA 3 cut(s) 890, 1765, 2826
TspRI CASTG 5 cut(s) 12, 138, 198, 705, 2481
Vha464I CTTAAG 2 cut(s) 1076, 2249
VneI GTGCAC 1 cut(s) 2732
VpaK11BI GGWCC 2 cut(s) 503, 2088
VspI ATTAAT 1 cut(s) 1823
XapI RAATTY 5 cut(s) 1063, 1307, 1771, 2285, 2547
XceI RCATGY 4 cut(s) 1043, 1851, 1855, 1963
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XmiI GTMKAC 2 cut(s) 787, 2785
XmnI GAANNNNTTC 1 cut(s) 2547
ZrmI AGTACT 1 cut(s) 1724
Zsp2I ATGCAT 2 cut(s) 1853, 2567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.