pycom13g25210

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000070
Physical Location & Seq
Forward (+)
22092029 .. 22096177
4149 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g25210.1

Sequence Viewer

Length: 4149 bp
ATGGCACTCGGAGAGGTTTTTCTTGCGGCGTTTCTACAGTTGCTGCTCGACAGGTTGACTCGTCGCGAGGTTCTCAACTATTTTGGAAACTTCCAGGGCGTTGGAAAGAAGCTGGACAAGTGGAGGGCCACGTTATCTACAATCGCAGCGGTGCTGAGCGACGCCGAGGAAAGACAACTGACTGACCATCTTGTAAAACTGTGGCTCGATGATCTCAGAGACCTGGCTTATGATGTCGAAGACATGTTGGACAAATTTGCCACTCAAATGTTGAAGCGTATGATAGAGGGACGTGTCAGTGCAAGCAACAAGGTGCGGAACTCACTTTTCAAAATTAAATTCAATTGGGATATGAACTCCGAAATGAAGAAGATTACGGAGCGGTTGCAAGACATATCTGAACGGAAAGACAAGTTTGGCTTGAAAGATACTGGGACGTCTGCTAAGGAATCGCAAAGTCTACCAAGTTCAGGCGTATTAGATGAAAAGCTTGTTGTTGGAAGAGATGGTGACAAAGGGGAGATTATTGAATTGTTGTCCAGAAAATACGAGCGTGCAGGTGCTGTCAATTTTGGTGTAGTTGCTATAGTTGGCCTGCCAGGAGTTGGGAAGACGACACTTGCTCAACTTGTATTCAACAACAAAGATGATGCCATAAAGGAGTTTGAGCTCAAGGTCTGGGTATCTGTGTCCGATGACTTCGATGTGGTGCGAGTGACAAAGGCAATTCTTCAATCAATCACATCCCAACCCGTTCAAGTGGAGGAGTTTAGTAAAATGCAGCATGATTTGAGTGAGCAATTAAGAGGAAAAAAGTTTTTAATTGTTTTAGATGATATTTGGGACAAAGATGACTATGATCTATACGATCTCTGGACAAGACTTCAATCCCCTTTTGGCGTCGGAGCAGGAGGAAGTAAGATAATTGTGACAACCCGTGACAGGAATGTTGCAAAGATTACGGGAGCCGCTGGAGTTCATAATTTGGAGTGTATGGCAGATGATGATTGTATGGAAATATTTGAGCGACACGCGTTTGGGGAAAATAATAACGGAAAACCAGTAAATTATGAGGTAATTCAGACGAAAATTGTTGGAAAATGTTGTGGATTACCATTAGCTGCAAGAACTCTCGGTGGTCTTTTACGTCGCCAAGAAAAAGAAGAGTGGGGAGAAATATTGGACAACAAATTATGGAATCTAGCAAATAAGAGTGGCCTTCTTCCTGTACTAAAGTTGAGCTATCACTATCTTCCATCACATTTGAAGAGGTGTTTTGCCTATTGCTCAATACTTCCAAATGACTATGAATTTGGGGAGAAGGAGCTCGTACTTTTGTGGATGGCAGAGGGTTTGATTCAACAAAAACATGACGACAATAAACAAATGGAGGATTTGGGCCGCGACTACTTTCGAGAGCTATTAGCAAGGTCGCTGTTTCAAGAATCAAGCAAAAACAATTCACGATATGTAATGCATGACCTCATTAATGATTTAGCACGATGGGCAGCAGGTGAAATATGTTTTAGATTGGAAGATAAGCAAGGTGATAACTTGCAAAGCACTTGCTTTCGAAGGGCTCGCCATTCGTCTTTCATTGCTGGTCAATTTGATGGAGTTATGAGATTTGAGGACTTTCCAAAAGCTGAAAGTTTACGAACATTCCTGCCACTTTCACTTTCAGATTCCAGGGGATGGTACAAATATTTGTCTCGTAAGGTTACTTTTGAGTTATTACCACAGTTGCAATACTTACGAGTGCTCTCTTTCAATGGCTACAAAATAACTGAGCTGCCAAAGTCAATCGGTGATTTGAGGTTGTTACAGTATCTTGACCTATCCTACACATTGATAACCAGTTTGCCCAAATCAACAAGCAGTCTTTACTACTTGCAAACATTGATATTGGAAGGTTGTTATAAATTGAAGTCATTGCCTGCGGACTTGAGCAATCTAATTAAGTTGCACCATCTCAACAACTCAGGTGCATATTCGTTGGAAGGAATGCCTTCGCAACTAGGTCGATTGACAAATCTACAATCACTGCCTCTTTTTGTGGTGAACGAAGGTAGTGATCAGTCAGGGATAAGAGAGATAGGGCCCCTATTGCATCTCCGAGGGACATTGTGCCTCTCAAAATTGGAGAATGTGATTGATGTTGAGGATGCTAGGAGGGCTAACTTGAAATGCAAGGAGAGGCTTGATTCGTTGGTCCTAGAATGGTCTCGTTCAAGGGACACAAGAGAAACGGAATCCACTGTGCTTGGCATGTTACAGCCTCATAGAAAGATCAAGGAGCTCACCATCAAGAGTTATGCCGAAAAGAAATTTTCATCATGGGTTGGAGGTTCTTTCTTCTCTAATATGGTGCTTGTGCGCTTAGAGGAATGTAACAATTGTTTATCGTTGCCACCTCTCGGACAATTGCCTCAACTCAAAGAGCTCTATATTAGAGGAATGAATGCGGTGGAAAGTGTTGGTGCTGAGTTTTATGGCGAGTGTGTCTTGGCTTTTCCGTTGTTAGAGATTCTCGAGTTTGTGGATATGCAGCATTGGAAGGTGTGGCTTCCCTTCCAACTGGATCACGGAAGTGGTGTTTTCCCTTGCCTGGAAAGGCTTTCAATCAAAAAATGTTCCAAGTTGGAAGGTAAGCTGCCAGAGAAGCTCGATTTGTTAGCCAAACTTGAAATTGTTAAATGTGAGGAATTGGTGGTTTCGATTGCCAACTACAAACAACTTCGTCAAATAAACATTGACGGTTGTAAAGTATTGGTACATACAGATGCTAAGGTTGAGTTTGAGTTATTAGAGTCCTTGTGGCTTTCAAACATTTCGGAGGTGATGTCTCTGCAAACAGGGGAATTGTTCAGGAAGGGATTAAGCAAGGTTAGAGATTTGAAGATCAGTGGATGTGAGAAGCTGACGTCTTCATGGAAGAATGAGGGTAGATTATTGCGGCGGTTGACTTGTCTTGGCCGTTTGGAAATTGAAGACAACTCTCGTCTAGTTGAAGAATTGGGAGAAGAAACAGAGGAGTTACTGCAATTGGAAATATTGGAGTGCAAGCTTGCATGTCTGGAGTTAAAAAAGTGCGGAAATCTTTTGAAGCTACCAAAAGGGTTAAATCAGCTGTCGTCTCTTCAAGAGCTTCGCATATACAAATGTTCATCTCTAGTTTCTTTTCCAGATGTTGGTCTGCCACCTTCTCTTAAAAACATCGAGATTATAGAGTGCGATTCGTTGATATATTTTGCAAAATTCCAGATTCCCCAAAATCTCAGAAGAATAGGGATAAAAGGTTGCAAAAGTTTGAAATCACTAGTAGATGAGGAGGGTGTTGGTTCTTCTCATGGTTGTCTTGAGTACTTGGATATCCAAGGATGTAAATCTCTGACGTCGTTATCATTGAGTGGTCAGCTCCTCAAGACACTTAAACACCTCGGGATATCTGGTTGTGAACAACTAGAGTTAATCGCACAGGACGGGTTCTTGTACGACAACACTAATTACTGTCTTGAATATTTTAGGATATGGATATGTGAAAATCTGAAATCCTTACCGGATGGCTTATGCCACCTCAGCAATCTTCAACAGCTACGTATTTACAAATGTGGAAGTCTTGTTTCCATCCCGAGACTGAGTGGGGGGAGAAGACCTTCCAACCTGAGACATTTGAGCATCGACTACCGTGAAGGTTTGACTTTTCCTCCCAACCTAACATCACTTGATATTAGGAAGGTAAAGAGCTATAAGTCATTGTGGGAGTTGGAATTGTGGGGGTTGCACAGACTCACCTCTCTTATAATGGATGGTGAAGAAGACCCGGATACGGTGTCGTTTCCACCCGACATGGTCCGCATGGAGACGCTCTTCCCCAAATCTCTCACTCGACTCAGAATAGTTGGCTTCCCGAATCTGAAGAAACTGAGCAGCAAGGGCTTTCAATTCCTCACCTCCCTTCAATCTCTGTGGCTCTTCAATTGTCCAAAGCTAGCATCCATTCCAGAAGAGGGTCTGCCTCCTTCACTAGAGGAACTTCGCATCATTAGGTGTCCAGTGCTAAAAGAGAGATGCCAACTAGGAAAAGGACACTACTGGCACAAAATATCCCACATCCCTTGCATAGAGATGGGTGTTAACGAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1383

Amino Acids

157.25

Weight (kDa)

6.93

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 100 1.7e-21 Rx N-terminal domain
NB-ARC PF00931 172 - 348 3.8e-36 NB-ARC domain
WHD_DRP PF23559 431 - 500 8.5e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 571 - 628 9.6e-09 Leucine-rich repeat region
LRR_14 PF23598 579 - 876 6.9e-23 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 696 - 820 5e-35 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 1116 - 1228 8.2e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1920, 3803
AarI CACCTGC 2 cut(s) 548, 1502
AatII GACGTC 3 cut(s) 440, 2927, 3398
Acc36I ACCTGC 2 cut(s) 548, 1502
AccB7I CCANNNNNTGG 3 cut(s) 605, 1695, 3191
AccBSI CCGCTC 1 cut(s) 382
AccI GTMKAC 1 cut(s) 460
AccII CGCG 3 cut(s) 66, 1034, 1404
AclWI GGATC 1 cut(s) 2589
AcoI YGGCCR 1 cut(s) 2974
AcsI RAATTY 5 cut(s) 254, 338, 1310, 2327, 3257
AcuI CTGAAG 1 cut(s) 3938
AcyI GRCGYC 5 cut(s) 162, 437, 900, 2924, 3395
AfaI GTAC 6 cut(s) 1230, 1332, 1700, 2775, 3365, 3494
AfiI CCNNNNNNNGG 9 cut(s) 470, 605, 942, 1695, 2417, 2608, 3191, 3829, 4010
AflIII ACRYGT 3 cut(s) 243, 292, 1032
AhlI ACTAGT 1 cut(s) 3319
AjiI CACGTC 1 cut(s) 293
AjnI CCWGG 5 cut(s) 93, 222, 598, 1688, 2607
AleI CACNNNNGTG 1 cut(s) 2589
Alw21I GWGCWC 5 cut(s) 672, 1329, 1764, 2301, 2445
Alw26I GTCTC 8 cut(s) 213, 1716, 2229, 2850, 3141, 3628, 3661, 3857
AlwI GGATC 1 cut(s) 2589
AlwNI CAGNNNCTG 2 cut(s) 43, 563
Ama87I CYCGRG 3 cut(s) 2531, 3440, 3631
AoxI GGCC 6 cut(s) 126, 592, 1216, 1399, 2099, 2974
ApaI GGGCCC 1 cut(s) 2103
ApeKI GCWGC 9 cut(s) 43, 146, 781, 1121, 1508, 1792, 2548, 2653, 3930
ApoI RAATTY 5 cut(s) 254, 338, 1310, 2327, 3257
ArsI GACNNNNNNTTYG 2 cut(s) 1295, 1327
AseI ATTAAT 1 cut(s) 1488
Asp700I GAANNNNTTC 2 cut(s) 2008, 3655
AspLEI GCGC 1 cut(s) 2379
AspS9I GGNCC 6 cut(s) 126, 1399, 2099, 2100, 2212, 3853
AsuC2I CCSGG 1 cut(s) 3824
AsuII TTCGAA 1 cut(s) 1573
AsuNHI GCTAGC 1 cut(s) 3991
AvaI CYCGRG 3 cut(s) 2531, 3440, 3631
AvaII GGWCC 2 cut(s) 2212, 3853
BaeGI GKGCMC 1 cut(s) 2103
BanII GRGCYC 6 cut(s) 672, 1329, 1582, 2103, 2301, 2445
BbsI GAAGAC 6 cut(s) 246, 617, 2919, 2997, 3658, 3825
Bbv12I GWGCWC 5 cut(s) 672, 1329, 1764, 2301, 2445
BbvCI CCTCAGC 1 cut(s) 3578
BbvI GCAGC 9 cut(s) 30, 158, 793, 1108, 1520, 1779, 2560, 2640, 3942
BceAI ACGGC 1 cut(s) 2961
BcgI CGANNNNNNTGC 2 cut(s) 2003, 2037
BciT130I CCWGG 5 cut(s) 95, 224, 600, 1690, 2609
BciVI GTATCC 1 cut(s) 3820
BclI TGATCA 1 cut(s) 2074
BcnI CCSGG 1 cut(s) 3824
BcoDI GTCTC 8 cut(s) 213, 1716, 2229, 2850, 3141, 3628, 3661, 3857
BcuI ACTAGT 1 cut(s) 3319
BfmI CTRYAG 2 cut(s) 35, 585
BfuAI ACCTGC 2 cut(s) 548, 1502
BfuI GTATCC 1 cut(s) 3820
BlpI GCTNAGC 1 cut(s) 155
BmcAI AGTACT 1 cut(s) 3365
Bme1390I CCNGG 6 cut(s) 95, 224, 600, 1690, 2609, 3824
Bme18I GGWCC 2 cut(s) 2212, 3853
BmeT110I CYCGRG 3 cut(s) 2531, 3440, 3631
BmgBI CACGTC 1 cut(s) 293
BmgT120I GGNCC 6 cut(s) 126, 1399, 2099, 2100, 2212, 3853
BmiI GGNNCC 3 cut(s) 967, 2101, 2102
BmrFI CCNGG 6 cut(s) 95, 224, 600, 1690, 2609, 3824
BmrI ACTGGG 1 cut(s) 441
BmsI GCATC 8 cut(s) 640, 2119, 2155, 2773, 3687, 4004, 4050, 4061
BmtI GCTAGC 1 cut(s) 3995
BmuI ACTGGG 1 cut(s) 441
BpiI GAAGAC 6 cut(s) 246, 617, 2919, 2997, 3658, 3825
BpmI CTGGAG 2 cut(s) 993, 3098
Bpu10I CCTNAGC 3 cut(s) 444, 2787, 3578
Bpu1102I GCTNAGC 1 cut(s) 155
Bpu14I TTCGAA 1 cut(s) 1573
BpuEI CTTGAG 4 cut(s) 656, 1966, 3380, 3407
BpuMI CCSGG 1 cut(s) 3824
BsaAI YACGTR 1 cut(s) 3599
BsaBI GATNNNNATC 1 cut(s) 3385
BsaHI GRCGYC 5 cut(s) 162, 437, 900, 2924, 3395
BsaI GGTCTC 2 cut(s) 213, 2229
BsaJI CCNNGG 6 cut(s) 94, 165, 1689, 2116, 3376, 3439
BsaWI WCCGGW 1 cut(s) 3559
BsaXI ACNNNNNCTCC 4 cut(s) 2828, 2858, 3691, 3721
Bsc4I CCNNNNNNNGG 9 cut(s) 470, 605, 942, 1695, 2417, 2608, 3191, 3829, 4010
Bse1I ACTGG 6 cut(s) 436, 1061, 1857, 2583, 4055, 4100
Bse3DI GCAATG 2 cut(s) 1596, 1931
Bse8I GATNNNNATC 1 cut(s) 3385
BseBI CCWGG 5 cut(s) 95, 224, 600, 1690, 2609
BseDI CCNNGG 6 cut(s) 94, 165, 1689, 2116, 3376, 3439
BseJI GATNNNNATC 1 cut(s) 3385
BseLI CCNNNNNNNGG 9 cut(s) 470, 605, 942, 1695, 2417, 2608, 3191, 3829, 4010
BseMI GCAATG 2 cut(s) 1596, 1931
BseNI ACTGG 6 cut(s) 436, 1061, 1857, 2583, 4055, 4100
BseRI GAGGAG 4 cut(s) 779, 3047, 3344, 3410
BseSI GKGCMC 1 cut(s) 2103
BseXI GCAGC 9 cut(s) 30, 158, 793, 1108, 1520, 1779, 2560, 2640, 3942
BsgI GTGCAG 1 cut(s) 576
Bsh1236I CGCG 3 cut(s) 66, 1034, 1404
BshFI GGCC 6 cut(s) 128, 594, 1218, 1401, 2101, 2976
BsiHKAI GWGCWC 5 cut(s) 672, 1329, 1764, 2301, 2445
BsiHKCI CYCGRG 3 cut(s) 2531, 3440, 3631
BsiSI CCGG 2 cut(s) 3560, 3824
BslFI GGGAC 5 cut(s) 303, 448, 857, 2134, 2249
BslI CCNNNNNNNGG 9 cut(s) 470, 605, 942, 1695, 2417, 2608, 3191, 3829, 4010
BsmAI GTCTC 8 cut(s) 213, 1716, 2229, 2850, 3141, 3628, 3661, 3857
BsmBI CGTCTC 2 cut(s) 3141, 3857
BsmFI GGGAC 5 cut(s) 303, 448, 857, 2134, 2249
BsmI GAATGC 2 cut(s) 2010, 2467
BsnI GGCC 6 cut(s) 128, 594, 1218, 1401, 2101, 2976
Bso31I GGTCTC 2 cut(s) 213, 2229
BsoBI CYCGRG 3 cut(s) 2531, 3440, 3631
Bsp119I TTCGAA 1 cut(s) 1573
Bsp120I GGGCCC 1 cut(s) 2099
Bsp1286I GDGCHC 7 cut(s) 672, 1329, 1582, 1764, 2103, 2301, 2445
Bsp143I GATC 7 cut(s) 211, 859, 868, 2074, 2289, 2581, 2901
Bsp1720I GCTNAGC 1 cut(s) 155
Bsp68I TCGCGA 1 cut(s) 66
BspANI GGCC 6 cut(s) 128, 594, 1218, 1401, 2101, 2976
BspFNI CGCG 3 cut(s) 66, 1034, 1404
BspLI GGNNCC 3 cut(s) 967, 2101, 2102
BspMI ACCTGC 2 cut(s) 548, 1502
BspOI GCTAGC 1 cut(s) 3995
BspPI GGATC 1 cut(s) 2589
BspQI GCTCTTC 2 cut(s) 3875, 3980
BspT104I TTCGAA 1 cut(s) 1573
BspTNI GGTCTC 2 cut(s) 213, 2229
BsrBI CCGCTC 1 cut(s) 382
BsrDI GCAATG 2 cut(s) 1596, 1931
BsrI ACTGG 6 cut(s) 436, 1061, 1857, 2583, 4055, 4100
BssECI CCNNGG 6 cut(s) 94, 165, 1689, 2116, 3376, 3439
BssMI GATC 7 cut(s) 211, 859, 868, 2074, 2289, 2581, 2901
BssNI GRCGYC 5 cut(s) 162, 437, 900, 2924, 3395
BssT1I CCWWGG 1 cut(s) 3376
Bst2UI CCWGG 5 cut(s) 95, 224, 600, 1690, 2609
Bst4CI ACNGT 9 cut(s) 39, 201, 1743, 1827, 2260, 2759, 3512, 3689, 3832
Bst6I CTCTTC 7 cut(s) 496, 1158, 1262, 3144, 3875, 3980, 4002
BstACI GRCGYC 5 cut(s) 162, 437, 900, 2924, 3395
BstBAI YACGTR 1 cut(s) 3599
BstBI TTCGAA 1 cut(s) 1573
BstC8I GCNNGC 8 cut(s) 304, 555, 596, 1582, 1938, 3065, 3069, 3993
BstFNI CGCG 3 cut(s) 66, 1034, 1404
BstHHI GCGC 1 cut(s) 2379
BstKTI GATC 7 cut(s) 214, 862, 871, 2077, 2292, 2584, 2904
BstMAI GTCTC 8 cut(s) 213, 1716, 2229, 2850, 3141, 3628, 3661, 3857
BstMBI GATC 7 cut(s) 211, 859, 868, 2074, 2289, 2581, 2901
BstMWI GCNNNNNNNGC 6 cut(s) 1505, 2107, 2174, 2662, 3579, 3936
BstNI CCWGG 5 cut(s) 95, 224, 600, 1690, 2609
BstNSI RCATGY 3 cut(s) 247, 2272, 3075
BstSCI CCNGG 6 cut(s) 93, 222, 598, 1688, 2607, 3822
BstSFI CTRYAG 2 cut(s) 35, 585
BstSLI GKGCMC 1 cut(s) 2103
BstSNI TACGTA 1 cut(s) 3599
BstUI CGCG 3 cut(s) 66, 1034, 1404
BstV1I GCAGC 9 cut(s) 30, 158, 793, 1108, 1520, 1779, 2560, 2640, 3942
BstV2I GAAGAC 6 cut(s) 246, 617, 2919, 2997, 3658, 3825
BstXI CCANNNNNNTGG 1 cut(s) 101
BsuI GTATCC 1 cut(s) 3820
BsuRI GGCC 6 cut(s) 128, 594, 1218, 1401, 2101, 2976
BtrI CACGTC 1 cut(s) 293
BtsI GCAGTG 1 cut(s) 2042
BtsIMutI CAGTG 5 cut(s) 304, 2042, 2256, 2911, 4062
BtuMI TCGCGA 1 cut(s) 66
BveI ACCTGC 2 cut(s) 548, 1502
Cac8I GCNNGC 8 cut(s) 304, 555, 596, 1582, 1938, 3065, 3069, 3993
CaiI CAGNNNCTG 2 cut(s) 43, 563
CfoI GCGC 1 cut(s) 2379
Cfr13I GGNCC 6 cut(s) 126, 1399, 2099, 2100, 2212, 3853
CseI GACGC 3 cut(s) 170, 889, 3874
Csp6I GTAC 6 cut(s) 1229, 1331, 1699, 2774, 3364, 3493
CspCI CAANNNNNGTGG 6 cut(s) 2245, 2280, 3950, 3985, 4100, 4135
CviQI GTAC 6 cut(s) 1229, 1331, 1699, 2774, 3364, 3493
DpnI GATC 7 cut(s) 213, 861, 870, 2076, 2291, 2583, 2903
DpnII GATC 7 cut(s) 211, 859, 868, 2074, 2289, 2581, 2901
EaeI YGGCCR 1 cut(s) 2974
Eam1104I CTCTTC 7 cut(s) 496, 1158, 1262, 3144, 3875, 3980, 4002
EarI CTCTTC 7 cut(s) 496, 1158, 1262, 3144, 3875, 3980, 4002
Ecl136II GAGCTC 4 cut(s) 670, 1327, 2299, 2443
Eco105I TACGTA 1 cut(s) 3599
Eco130I CCWWGG 1 cut(s) 3376
Eco24I GRGCYC 6 cut(s) 672, 1329, 1582, 2103, 2301, 2445
Eco31I GGTCTC 2 cut(s) 213, 2229
Eco32I GATATC 2 cut(s) 3373, 3447
Eco47I GGWCC 2 cut(s) 2212, 3853
Eco53kI GAGCTC 4 cut(s) 670, 1327, 2299, 2443
Eco57I CTGAAG 1 cut(s) 3938
Eco88I CYCGRG 3 cut(s) 2531, 3440, 3631
EcoICRI GAGCTC 4 cut(s) 670, 1327, 2299, 2443
EcoO109I RGGNCCY 2 cut(s) 2099, 2100
EcoRII CCWGG 5 cut(s) 93, 222, 598, 1688, 2607
EcoRV GATATC 2 cut(s) 3373, 3447
EcoT14I CCWWGG 1 cut(s) 3376
EcoT22I ATGCAT 1 cut(s) 1479
EcoT38I GRGCYC 6 cut(s) 672, 1329, 1582, 2103, 2301, 2445
ErhI CCWWGG 1 cut(s) 3376
Esp3I CGTCTC 2 cut(s) 3141, 3857
FalI AAGNNNNNCTT 2 cut(s) 404, 436
FaqI GGGAC 5 cut(s) 303, 448, 857, 2134, 2249
FbaI TGATCA 1 cut(s) 2074
FblI GTMKAC 1 cut(s) 460
FriOI GRGCYC 6 cut(s) 672, 1329, 1582, 2103, 2301, 2445
GlaI GCGC 1 cut(s) 2378
GsuI CTGGAG 2 cut(s) 993, 3098
HaeIII GGCC 6 cut(s) 128, 594, 1218, 1401, 2101, 2976
HapII CCGG 2 cut(s) 3560, 3824
HgaI GACGC 3 cut(s) 170, 889, 3874
HhaI GCGC 1 cut(s) 2379
Hin1I GRCGYC 5 cut(s) 162, 437, 900, 2924, 3395
Hin6I GCGC 1 cut(s) 2377
HinP1I GCGC 1 cut(s) 2377
HincII GTYRAC 3 cut(s) 57, 2964, 4138
HindII GTYRAC 3 cut(s) 57, 2964, 4138
HindIII AAGCTT 2 cut(s) 488, 3065
HpaI GTTAAC 1 cut(s) 4138
HpaII CCGG 2 cut(s) 3560, 3824
Hpy166II GTNNAC 7 cut(s) 57, 461, 1655, 2062, 2964, 3458, 4138
Hpy8I GTNNAC 7 cut(s) 57, 461, 1655, 2062, 2964, 3458, 4138
Hpy99I CGWCG 5 cut(s) 66, 164, 905, 1152, 3400
HpyCH4III ACNGT 9 cut(s) 39, 201, 1743, 1827, 2260, 2759, 3512, 3689, 3832
HpyCH4IV ACGT 7 cut(s) 131, 292, 437, 1147, 2924, 3395, 3598
HpyF10VI GCNNNNNNNGC 6 cut(s) 1505, 2107, 2174, 2662, 3579, 3936
HpySE526I ACGT 7 cut(s) 131, 292, 437, 1147, 2924, 3395, 3598
Hsp92I GRCGYC 5 cut(s) 162, 437, 900, 2924, 3395
HspAI GCGC 1 cut(s) 2377
Ksp22I TGATCA 1 cut(s) 2074
KspAI GTTAAC 1 cut(s) 4138
Kzo9I GATC 7 cut(s) 211, 859, 868, 2074, 2289, 2581, 2901
LguI GCTCTTC 2 cut(s) 3875, 3980
LmnI GCTCC 6 cut(s) 379, 905, 965, 1324, 2296, 3423
Lsp1109I GCAGC 9 cut(s) 30, 158, 793, 1108, 1520, 1779, 2560, 2640, 3942
LweI GCATC 8 cut(s) 640, 2119, 2155, 2773, 3687, 4004, 4050, 4061
MaeII ACGT 7 cut(s) 131, 292, 437, 1147, 2924, 3395, 3598
MaeIII GTNAC 9 cut(s) 509, 715, 928, 938, 1720, 1821, 2271, 2390, 3036
MalI GATC 7 cut(s) 213, 861, 870, 2076, 2291, 2583, 2903
MbiI CCGCTC 1 cut(s) 382
MboI GATC 7 cut(s) 211, 859, 868, 2074, 2289, 2581, 2901
MfeI CAATTG 5 cut(s) 343, 2395, 2423, 3044, 3979
MhlI GDGCHC 7 cut(s) 672, 1329, 1582, 1764, 2103, 2301, 2445
MluI ACGCGT 1 cut(s) 1032
MlyI GAGTC 4 cut(s) 52, 2819, 3783, 3885
Mph1103I ATGCAT 1 cut(s) 1479
MroXI GAANNNNTTC 2 cut(s) 2008, 3655
MslI CAYNNNNRTG 4 cut(s) 266, 2589, 2781, 4127
MspA1I CMGCKG 3 cut(s) 149, 971, 3130
MspI CCGG 2 cut(s) 3560, 3824
MspR9I CCNGG 6 cut(s) 95, 224, 600, 1690, 2609, 3824
MunI CAATTG 5 cut(s) 343, 2395, 2423, 3044, 3979
Mva1269I GAATGC 2 cut(s) 2010, 2467
MvaI CCWGG 5 cut(s) 95, 224, 600, 1690, 2609
MvnI CGCG 3 cut(s) 66, 1034, 1404
MwoI GCNNNNNNNGC 6 cut(s) 1505, 2107, 2174, 2662, 3579, 3936
NciI CCSGG 1 cut(s) 3824
NdeII GATC 7 cut(s) 211, 859, 868, 2074, 2289, 2581, 2901
NheI GCTAGC 1 cut(s) 3991
NlaIV GGNNCC 3 cut(s) 967, 2101, 2102
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 4 cut(s) 509, 715, 928, 938
NruI TCGCGA 1 cut(s) 66
NsiI ATGCAT 1 cut(s) 1479
NspI RCATGY 3 cut(s) 247, 2272, 3075
NspV TTCGAA 1 cut(s) 1573
OliI CACNNNNGTG 1 cut(s) 2589
PaeR7I CTCGAG 1 cut(s) 2531
PaqCI CACCTGC 2 cut(s) 548, 1502
PciI ACATGT 1 cut(s) 243
PciSI GCTCTTC 2 cut(s) 3875, 3980
PctI GAATGC 2 cut(s) 2010, 2467
PdmI GAANNNNTTC 2 cut(s) 2008, 3655
PflFI GACNNNGTC 1 cut(s) 3851
PflMI CCANNNNNTGG 3 cut(s) 605, 1695, 3191
PleI GAGTC 4 cut(s) 52, 2818, 3783, 3885
PpsI GAGTC 4 cut(s) 52, 2818, 3783, 3885
Ppu21I YACGTR 1 cut(s) 3599
PscI ACATGT 1 cut(s) 243
PshBI ATTAAT 1 cut(s) 1488
PsiI TTATAA 2 cut(s) 1920, 3803
Psp124BI GAGCTC 4 cut(s) 672, 1329, 2301, 2445
Psp6I CCWGG 5 cut(s) 93, 222, 598, 1688, 2607
PspGI CCWGG 5 cut(s) 93, 222, 598, 1688, 2607
PspN4I GGNNCC 3 cut(s) 967, 2101, 2102
PspOMI GGGCCC 1 cut(s) 2099
PspPI GGNCC 6 cut(s) 126, 1399, 2099, 2100, 2212, 3853
PstNI CAGNNNCTG 2 cut(s) 43, 563
PsyI GACNNNGTC 1 cut(s) 3851
PvuII CAGCTG 1 cut(s) 3130
RruI TCGCGA 1 cut(s) 66
RsaI GTAC 6 cut(s) 1230, 1332, 1700, 2775, 3365, 3494
RsaNI GTAC 6 cut(s) 1229, 1331, 1699, 2774, 3364, 3493
RseI CAYNNNNRTG 4 cut(s) 266, 2589, 2781, 4127
SacI GAGCTC 4 cut(s) 672, 1329, 2301, 2445
SapI GCTCTTC 2 cut(s) 3875, 3980
Sau3AI GATC 7 cut(s) 211, 859, 868, 2074, 2289, 2581, 2901
Sau96I GGNCC 6 cut(s) 126, 1399, 2099, 2100, 2212, 3853
ScaI AGTACT 1 cut(s) 3365
SchI GAGTC 4 cut(s) 52, 2819, 3783, 3885
ScrFI CCNGG 6 cut(s) 95, 224, 600, 1690, 2609, 3824
SduI GDGCHC 7 cut(s) 672, 1329, 1582, 1764, 2103, 2301, 2445
SfaNI GCATC 8 cut(s) 640, 2119, 2155, 2773, 3687, 4004, 4050, 4061
SfcI CTRYAG 2 cut(s) 35, 585
Sfr274I CTCGAG 1 cut(s) 2531
SfuI TTCGAA 1 cut(s) 1573
SinI GGWCC 2 cut(s) 2212, 3853
SlaI CTCGAG 1 cut(s) 2531
SmiMI CAYNNNNRTG 4 cut(s) 266, 2589, 2781, 4127
SmlI CTYRAG 5 cut(s) 671, 1945, 2531, 3359, 3422
SmoI CTYRAG 5 cut(s) 671, 1945, 2531, 3359, 3422
SnaBI TACGTA 1 cut(s) 3599
SpeI ACTAGT 1 cut(s) 3319
SspI AATATT 5 cut(s) 1020, 1179, 1706, 3054, 3521
SstI GAGCTC 4 cut(s) 672, 1329, 2301, 2445
StyD4I CCNGG 6 cut(s) 93, 222, 598, 1688, 2607, 3822
StyI CCWWGG 1 cut(s) 3376
TaaI ACNGT 9 cut(s) 39, 201, 1743, 1827, 2260, 2759, 3512, 3689, 3832
TaiI ACGT 7 cut(s) 134, 295, 440, 1150, 2927, 3398, 3601
TatI WGTACW 2 cut(s) 1228, 3363
TauI GCSGC 4 cut(s) 29, 971, 1404, 2959
TscAI CASTG 5 cut(s) 304, 2049, 2263, 2911, 4062
TseFI GTSAC 4 cut(s) 509, 715, 928, 938
TseI GCWGC 9 cut(s) 43, 146, 781, 1121, 1508, 1792, 2548, 2653, 3930
Tsp45I GTSAC 4 cut(s) 509, 715, 928, 938
TspGWI ACGGA 6 cut(s) 392, 418, 1068, 2264, 2505, 2601
TspRI CASTG 5 cut(s) 304, 2049, 2263, 2911, 4062
Tth111I GACNNNGTC 1 cut(s) 3851
Van91I CCANNNNNTGG 3 cut(s) 605, 1695, 3191
VpaK11BI GGWCC 2 cut(s) 2212, 3853
VspI ATTAAT 1 cut(s) 1488
XapI RAATTY 5 cut(s) 254, 338, 1310, 2327, 3257
XceI RCATGY 3 cut(s) 247, 2272, 3075
XhoI CTCGAG 1 cut(s) 2531
XmiI GTMKAC 1 cut(s) 460
XmnI GAANNNNTTC 2 cut(s) 2008, 3655
ZraI GACGTC 3 cut(s) 438, 2925, 3396
ZrmI AGTACT 1 cut(s) 3365
Zsp2I ATGCAT 1 cut(s) 1479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.