FvH4_7g27355

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000070
Physical Location & Seq
Reverse (-)
20479470 .. 20483924
4455 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g27355.t1

Sequence Viewer

Length: 4245 bp
ATGGGGGAGGCTGTTCTTGGTGCATTCCTCCCGGTGCTGCTTAAGAAGTTGGCGGATCAAGAGATCACTGAGTACTTCGGACGCCTTAAGGGGGTCGACAAAAAGGTGCTGGAGAAATGGACTAGAACGTTGACGGCCGTTGAAGCGGTGCTGAGTGATGCTGAGGAGAAGCAACTAACACAAAGTCGAGTGAAACTGTGGCTAGATGATCTCAGAGATTTGGCTTACGATGTTCAAGATGTATTGGACATATTTGATACTAAAATGCTGAAGCGGAGGATAGAAAGACAACAAGGAAGCAAAAGCAAGTTATGGAGCTCACTCTCTAAAGTTAAGTCCAATTTTGATCTGAACTCAGCAATCAAGAAGATAAGTGATCGACTTGAGGAGATAACTACTCGAAAGAAGCAGTTTGGTTTGAAGGAACTTGATAGACCCACTAAAAAGCCATGGAAAATGCCACTCACTACTTCTCAACCTGATGGACCTGTGATCGGAAGGGATGAAGCAAACGATAAGATTCTCTATGATTTGCTTTCAAAAGAAGAGAAGTATCATGTGGTTGGTATTGTCGGTACTGGAGGACTAGGAAAGACAACACTTGCTAAACATGTATTCAACGATGCTGCAACTGGCCAGTTTTCCCCAAAAGGATGGGTATCTGTGTCAGACGACTTTGACATTGTAAGGGTGGCAGCAGCCGTTTTGGAATCAGTCACGTCAGCTCCAGTTCAGGTCAAGGAGCTTAACTCAATTCTGGAGAAGTTAAGTAAGGAATTGGCTGGCAAGAAGTTTTTAATTGTTCTTGATGATGTCTGGGACACTTGTAGCTATGGTCATTGGACTACACTGCAGGCCTCCTTTCGTGTTGGAGCAGCTGGAAGTAAGATCATCGTGACAACACGTGACGCCAATGTCGCAAATATGATGGGAGATAGGAGCCCTTATAACTTGAAGCCGATGTCAGAAGATGATTGTTGGAGACTCTTTCAGCACCATGCTCTCTTAAATAACAGACCTGAAGATGTTGAGTTCCTTAAAGGAAAAATTGTAGAAAAATGCAACGGGCTACCGTTAGCTGCAAGGACTCTTGGTGGGCTTTTACGTTGTAAAGAAGTAGACGAATGGGAAGACATATTAGACCACAAGATGTGGACTCTATCAGACCAGAACAAGATGGATATCCTTCCAGCATTAAAACTGAGCTATCATTATCTCCCTTCGACTTTGAAACGGTGTTTCTCCTACTGTGCAATACTTCCAAATGATTATGAATTTAAGGAGATGCAATTGATCCTTTGGTGGATGGCAGAAGGTTTGATTCCGCAGCAACCCAAAGAACATAAGCAAATGGAAGATTTGGGACGTGACTATTTTCAAGAGTTGGTGTCCAGGTCATTATTTCAAAAATCAAGCAAAACAGAGTCGCGATACATAATGCATGACCTGGTTACTGATTTGGCTCGTTGGGCTGCAGGAAGTTCATGTTGTAGATTGGAGGACATGCAGAATTATGACTCGCAGCAACATTCATGTTTGCCCAAGATTCGTCATTCATCTTACATTCTTGGAGTGTGTGACGGGGTTAAGAAGTTCGAGGTCTATTCTGAAGCTATATGTTTGCGGACATTCTTACCACTTTCACTTGGGTCACTTCCAGATTCTCATTATCTGGCTCAGAAGGTTACTTCTAATTTATTTCCCAAACTCCAATACTTACGACTCCTCTCTTTGAAAAGTTATCAAATAATCGAGTTGCCAGATACAATTGGTGAATTGAAGCATCTACGGTATCTTGATCTCTCCCACACTGGGATAAGAAGTTTGCCAGACTCGACAACAACTCTGTACAACTTGCAGACATTGTTATTGGAAAGTTGTCACCAGTTGAAGGCACTGCCCACAAGCATGAGGAACCTAGTTAATTTGCGTCATCTCAACAATTCAGGTACACGGTCATTGGAAGAAATGCCTCCTCAGCTTGGTCAATTGACTAACCTCCAAACATTGCCTGAGTTTGTTATTGGCAAAGGTAGTGGATCAGGAGTAAGAGAGATCGAGTCATTATTGCATCTGCAAGGGACATTGCACATCTCAAGACTAGAAAATGTGATTTGTGTCGAGGATGCCAGGAGTGCCAACTGCTTAAAGAGCAAGGAAAGGCTTGAAGCCCTATTTCTTGAATGGTCTTCTTCGAGTGTCTCAACAGAAGATGCAGCAATTGTGCTTGACATGTTACAGCCTCATAGCAAGCTCAAAAAGCTCACCATCAAAGATTATGGTGGATCGAAATTTTCAACATGGATTGGGAATCCTTCATTCACAAAAATGGTGCGGGTAAAGTTAGAAGGTTGTAATCATTGTCAATTGTTGCCACCATTCGGACAGTTGCCTTGTCTCAAAGAACTTTGTATACAAGAAATGGATGCAGTGGAGAGTGTTGGTGTTGAGTTTTATGGAGAAGGTAAATTGTCGTTTCGAGCTTTAGAGACCCTGGAGTTACATAATCTGAAAAATTGGAAGAAGTGGTATCCTTGCCAACAAAGTGAGGGTGTTGAAGTTTTCTCTTGCCTGAAAAAGCTTTCCATCAGGGGCTGCCCCAAATTGGAGGGTGATTTACCAGAGAAGCTTGATTCATTAGCACAGCTTGAGATATATGGATGTGAGGAATTGGTGGTTTCAGTATCTAAATACAAACAGCTTCATGGATCAAACATTGAAAAGTGCAAGATGGTGGTGTACGACACAAGTACAGTTCAGTTTGAGTTACTAGAGAGTTTGACATATTCAAATGTATCAGAATTGAGATTCCAAACAGAGTCGTTCATGAAAAGCTTGAAAAATGTTAAAGAGTTGAAGATCACTCGTTGTGAGGAGCTTACATGTTGCTTTCAAAACGAGGATAGATTACTGCAACACTTGATTTCTCTTAGTCGTCTGTATATCGAAGACAACTCGATCCTTGTTGAAAAGCTAGGGATTGAAGCAGAGCAGTTGTTGCAATTGCAAATCCTGGATTGCAATATTGAACGTCTGGAATTAAGCAAGTGTGGAAGCCTTGTGAAGGTACCAGAAGGATTGCATCACCTTAGATCACTTCAAGAGCTGCACATAAGTGGATGTTCAAGTCTTGTTTCTTTTCCGGATGTTGGTCTGCCACCTTGTCTTCAAGAGCTGCACATAAGTGAATGTTCAAGTCTTGTTTCTTTTCCGGATGTTGGTCTGCCACCTTGTCTCCAAGACATAGTGATTCAGGACTGCGAATCTCTGTTGCACTTTGCAAAATACCAGATTCCTCCCAGCCTAAAAAGAATAGATATCTGGAATTGTGGGAATTTGAAGTCATTAATAGAGAAAGAGGAGGAGGTGGGTAACGGTTCTTCTTCTTCTTGTCTTGAGAGTTTGTGGATAAGGAATTGTCCCTCTTCGATGTGCTTATTATGCAAAGGTCAATTACCCGGGTCTCTTAAACAACTTGTGATACTGCGGTGTGGACAGCTGGAGTTAATAACTGAGAGGTTCCTCGATGACAGCTGCCAACTCGAAGAAATTGACATCGAGAACTGCCCAAACCTGAAATCCTTACCCGAGGGACTGTGCCACCTCACCAATCTTCAGTCATTAATTGTTTATGATTGTCAAAGTCTTGTTTCGTTGCCGAGAATGAATGTGTGGCCGAGAATGATAGGGATCAGAAATTGCGATAAATTGGAGGTGGCACAGTTGGCGAGAGACATGATGCACACCAGTAATCTTGAAGATCTGAGAATTGATTACTTTGAAGGTTTGACAACTACAACTTCCTTCCCCACCAACCTAACATCACTGTCAATTGACAAGATCAAGAATTGTAAGGCGCTGATGGAGTTTCAAGGGTTGCAGAGACTCGCCTCTTTGAGAGAATTGTATCTATGGGGTGAAGATGATGGAGGTCTGGTGTCATTTCCACCTGCGGCTGAGAATTCAAAGGAGACGGAGATACTGCTCCCCAGATCTCTTGTTGATTTGAGTATTGTAGACTTCCCAAATCTAAAGAAACTGAGCAAGGGCTTTCAATTCCTCACCTCTCTTGAACGTCTTTGGATCTGGAACTGTCCGAAGCTTACAACCCTTCCAGTGGAGGAAGGCCTGCCTCTTTCACTTCACACATCCCTGAAGTACGAATTAATCTGGAACTCATATAAGAGAAAACCAACACTCCTCGATGGAGTGGTTTCCAGGTCTCCTAATGCTCAAAACTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1415

Amino Acids

160.18

Weight (kDa)

6.03

Isoelectric Point (pI)

49.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 100 1.9e-19 Rx N-terminal domain
NB-ARC PF00931 172 - 335 1.2e-35 NB-ARC domain
WHD_DRP PF23559 419 - 488 1.1e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 567 - 837 9e-23 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 684 - 808 4.4e-35 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 948
AarI CACCTGC 1 cut(s) 3958
AasI GACNNNNNNGTC 1 cut(s) 914
Acc36I ACCTGC 1 cut(s) 3958
Acc65I GGTACC 1 cut(s) 3067
AccB1I GGYRCC 1 cut(s) 3067
AccI GTMKAC 4 cut(s) 96, 1119, 2413, 4017
AccII CGCG 1 cut(s) 1429
AccIII TCCGGA 2 cut(s) 3142, 3211
AciI CCGC 8 cut(s) 53, 146, 274, 1325, 1624, 2335, 3487, 3953
AclI AACGTT 1 cut(s) 128
AclWI GGATC 8 cut(s) 63, 1288, 2047, 2293, 2716, 2953, 3698, 4091
AcoI YGGCCR 3 cut(s) 135, 634, 3674
AcsI RAATTY 4 cut(s) 1274, 2291, 3334, 3961
AcuI CTGAAG 5 cut(s) 290, 1041, 1629, 3599, 4175
AcvI CACGTG 1 cut(s) 905
AcyI GRCGYC 2 cut(s) 82, 909
AfaI GTAC 8 cut(s) 74, 577, 1850, 1951, 2741, 2752, 3069, 4160
AflII CTTAAG 2 cut(s) 41, 86
AflIII ACRYGT 4 cut(s) 610, 902, 2232, 2882
AjiI CACGTC 2 cut(s) 720, 1367
AjnI CCWGG 6 cut(s) 1391, 1446, 2129, 2493, 3012, 4217
AleI CACNNNNGTG 2 cut(s) 3114, 3183
Alw21I GWGCWC 1 cut(s) 320
AlwI GGATC 8 cut(s) 63, 1288, 2047, 2293, 2716, 2953, 3698, 4091
AlwNI CAGNNNCTG 1 cut(s) 2595
Ama87I CYCGRG 2 cut(s) 3458, 3587
Aor13HI TCCGGA 2 cut(s) 3142, 3211
AoxI GGCC 5 cut(s) 135, 634, 855, 3674, 4126
ApoI RAATTY 4 cut(s) 1274, 2291, 3334, 3961
AseI ATTAAT 3 cut(s) 3347, 3623, 4166
Asp718I GGTACC 1 cut(s) 3067
AspLEI GCGC 1 cut(s) 3859
AspS9I GGNCC 1 cut(s) 485
AsuC2I CCSGG 3 cut(s) 32, 3459, 3460
AsuHPI GGTGA 8 cut(s) 1784, 1874, 2257, 2624, 3077, 3598, 3929, 4054
AvaI CYCGRG 2 cut(s) 3458, 3587
AvaII GGWCC 1 cut(s) 485
BaeI ACNNNNGTAYC 2 cut(s) 3473, 3506
BalI TGGCCA 1 cut(s) 636
BanI GGYRCC 1 cut(s) 3067
BanII GRGCYC 2 cut(s) 320, 944
BarI GAAGNNNNNNTAC 2 cut(s) 3364, 3396
BbrPI CACGTG 1 cut(s) 905
BbsI GAAGAC 4 cut(s) 1137, 2181, 2955, 3158
Bbv12I GWGCWC 1 cut(s) 320
BbvCI CCTCAGC 2 cut(s) 162, 1977
BceAI ACGGC 3 cut(s) 122, 150, 686
BciT130I CCWGG 6 cut(s) 1393, 1448, 2131, 2495, 3014, 4219
BciVI GTATCC 1 cut(s) 2541
BcnI CCSGG 3 cut(s) 32, 3459, 3460
BfaI CTAG 7 cut(s) 123, 203, 587, 1919, 2102, 2771, 2975
BfmI CTRYAG 2 cut(s) 851, 1473
BfoI RGCGCY 1 cut(s) 3860
BfrI CTTAAG 2 cut(s) 41, 86
BfuAI ACCTGC 1 cut(s) 3958
BfuI GTATCC 1 cut(s) 2541
BglII AGATCT 2 cut(s) 3760, 3992
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 9 cut(s) 32, 1393, 1448, 2131, 2495, 3014, 3459, 3460, 4219
Bme18I GGWCC 1 cut(s) 485
BmeT110I CYCGRG 2 cut(s) 3458, 3587
BmgBI CACGTC 2 cut(s) 720, 1367
BmgT120I GGNCC 1 cut(s) 485
BmiI GGNNCC 4 cut(s) 941, 1916, 3069, 3521
BmrFI CCNGG 9 cut(s) 32, 1393, 1448, 2131, 2495, 3014, 3459, 3460, 4219
BmrI ACTGGG 1 cut(s) 1821
BmuI ACTGGG 1 cut(s) 1821
BpiI GAAGAC 4 cut(s) 1137, 2181, 2955, 3158
BplI GAGNNNNNCTC 2 cut(s) 734, 766
BpmI CTGGAG 6 cut(s) 131, 600, 711, 779, 2516, 3521
Bpu10I CCTNAGC 2 cut(s) 162, 1977
BpuEI CTTGAG 4 cut(s) 404, 2080, 2669, 3416
BpuMI CCSGG 3 cut(s) 32, 3459, 3460
BsaAI YACGTR 1 cut(s) 905
BsaBI GATNNNNATC 3 cut(s) 658, 1181, 3689
BsaHI GRCGYC 2 cut(s) 82, 909
BsaI GGTCTC 3 cut(s) 2483, 3468, 4227
BsaJI CCNNGG 4 cut(s) 449, 2493, 3458, 3588
BsaWI WCCGGW 2 cut(s) 3142, 3211
BsaXI ACNNNNNCTCC 8 cut(s) 709, 739, 3219, 3249, 3921, 3951, 4182, 4212
Bse1I ACTGG 8 cut(s) 583, 637, 637, 728, 1816, 1885, 3747, 4115
Bse3DI GCAATG 2 cut(s) 2006, 2084
Bse8I GATNNNNATC 3 cut(s) 658, 1181, 3689
BseAI TCCGGA 2 cut(s) 3142, 3211
BseBI CCWGG 6 cut(s) 1393, 1448, 2131, 2495, 3014, 4219
BseDI CCNNGG 4 cut(s) 449, 2493, 3458, 3588
BseJI GATNNNNATC 3 cut(s) 658, 1181, 3689
BseMI GCAATG 2 cut(s) 2006, 2084
BseNI ACTGG 8 cut(s) 583, 637, 637, 728, 1816, 1885, 3747, 4115
BseRI GAGGAG 8 cut(s) 179, 401, 1715, 1965, 2888, 3374, 3377, 4190
BseX3I CGGCCG 1 cut(s) 135
BseYI CCCAGC 1 cut(s) 3299
BsgI GTGCAG 2 cut(s) 3092, 3161
Bsh1236I CGCG 1 cut(s) 1429
Bsh1285I CGRYCG 1 cut(s) 138
BshFI GGCC 5 cut(s) 137, 636, 857, 3676, 4128
BshNI GGYRCC 1 cut(s) 3067
BsiEI CGRYCG 1 cut(s) 138
BsiHKAI GWGCWC 1 cut(s) 320
BsiHKCI CYCGRG 2 cut(s) 3458, 3587
BsiSI CCGG 4 cut(s) 32, 3143, 3212, 3459
BslFI GGGAC 5 cut(s) 833, 1377, 2095, 3405, 3606
BsmBI CGTCTC 1 cut(s) 3965
BsmFI GGGAC 5 cut(s) 833, 1377, 2095, 3405, 3606
BsmI GAATGC 1 cut(s) 23
BsnI GGCC 5 cut(s) 137, 636, 857, 3676, 4128
Bso31I GGTCTC 3 cut(s) 2483, 3468, 4227
BsoBI CYCGRG 2 cut(s) 3458, 3587
Bsp1286I GDGCHC 2 cut(s) 320, 944
Bsp13I TCCGGA 2 cut(s) 3142, 3211
Bsp1407I TGTACA 1 cut(s) 1848
Bsp19I CCATGG 1 cut(s) 449
Bsp68I TCGCGA 1 cut(s) 1429
BspACI CCGC 8 cut(s) 53, 146, 274, 1325, 1624, 2335, 3487, 3953
BspANI GGCC 5 cut(s) 137, 636, 857, 3676, 4128
BspEI TCCGGA 2 cut(s) 3142, 3211
BspFNI CGCG 1 cut(s) 1429
BspHI TCATGA 1 cut(s) 2826
BspLI GGNNCC 4 cut(s) 941, 1916, 3069, 3521
BspMAI CTGCAG 2 cut(s) 855, 1477
BspMI ACCTGC 1 cut(s) 3958
BspPI GGATC 8 cut(s) 63, 1288, 2047, 2293, 2716, 2953, 3698, 4091
BspT107I GGYRCC 1 cut(s) 3067
BspTI CTTAAG 2 cut(s) 41, 86
BspTNI GGTCTC 3 cut(s) 2483, 3468, 4227
BsrDI GCAATG 2 cut(s) 2006, 2084
BsrGI TGTACA 1 cut(s) 1848
BsrI ACTGG 8 cut(s) 583, 637, 637, 728, 1816, 1885, 3747, 4115
BssECI CCNNGG 4 cut(s) 449, 2493, 3458, 3588
BssNAI GTATAC 1 cut(s) 2414
BssNI GRCGYC 2 cut(s) 82, 909
BssT1I CCWWGG 1 cut(s) 449
Bst1107I GTATAC 1 cut(s) 2414
Bst2UI CCWGG 6 cut(s) 1393, 1448, 2131, 2495, 3014, 4219
Bst6I CTCTTC 2 cut(s) 540, 3430
BstACI GRCGYC 2 cut(s) 82, 909
BstAFI CTTAAG 2 cut(s) 41, 86
BstAPI GCANNNNNTGC 1 cut(s) 2998
BstAUI TGTACA 1 cut(s) 1848
BstBAI YACGTR 1 cut(s) 905
BstC8I GCNNGC 4 cut(s) 784, 855, 2252, 4130
BstDSI CCRYGG 1 cut(s) 449
BstENI CCTNNNNNAGG 1 cut(s) 3062
BstFNI CGCG 1 cut(s) 1429
BstH2I RGCGCY 1 cut(s) 3860
BstHHI GCGC 1 cut(s) 3859
BstMCI CGRYCG 1 cut(s) 138
BstNI CCWGG 6 cut(s) 1393, 1448, 2131, 2495, 3014, 4219
BstNSI RCATGY 4 cut(s) 614, 1507, 2236, 2886
BstSCI CCNGG 9 cut(s) 30, 1391, 1446, 2129, 2493, 3012, 3457, 3458, 4217
BstSFI CTRYAG 2 cut(s) 851, 1473
BstUI CGCG 1 cut(s) 1429
BstV2I GAAGAC 4 cut(s) 1137, 2181, 2955, 3158
BstX2I RGATCY 3 cut(s) 3760, 3992, 4083
BstXI CCANNNNNNTGG 1 cut(s) 654
BstYI RGATCY 3 cut(s) 3760, 3992, 4083
BstZ17I GTATAC 1 cut(s) 2414
BstZI CGGCCG 1 cut(s) 135
BsuI GTATCC 1 cut(s) 2541
BsuRI GGCC 5 cut(s) 137, 636, 857, 3676, 4128
BtgI CCRYGG 1 cut(s) 449
BtrI CACGTC 2 cut(s) 720, 1367
BtsI GCAGTG 3 cut(s) 848, 1895, 2436
BtsIMutI CAGTG 7 cut(s) 66, 848, 1809, 1895, 2436, 3824, 4122
BtuMI TCGCGA 1 cut(s) 1429
BveI ACCTGC 1 cut(s) 3958
Cac8I GCNNGC 4 cut(s) 784, 855, 2252, 4130
CaiI CAGNNNCTG 1 cut(s) 2595
CciI TCATGA 1 cut(s) 2826
CfoI GCGC 1 cut(s) 3859
Cfr13I GGNCC 1 cut(s) 485
Cfr9I CCCGGG 1 cut(s) 3458
CseI GACGC 3 cut(s) 90, 917, 1919
CsiI ACCWGGT 1 cut(s) 1446
Csp6I GTAC 8 cut(s) 73, 576, 1849, 1950, 2740, 2751, 3068, 4159
CspCI CAANNNNNGTGG 2 cut(s) 2017, 2052
CviQI GTAC 8 cut(s) 73, 576, 1849, 1950, 2740, 2751, 3068, 4159
DrdI GACNNNNNNGTC 1 cut(s) 914
DseDI GACNNNNNNGTC 1 cut(s) 914
EaeI YGGCCR 3 cut(s) 135, 634, 3674
EagI CGGCCG 1 cut(s) 135
Eam1104I CTCTTC 2 cut(s) 540, 3430
EarI CTCTTC 2 cut(s) 540, 3430
EciI GGCGGA 1 cut(s) 68
Ecl136II GAGCTC 1 cut(s) 318
EclXI CGGCCG 1 cut(s) 135
Eco130I CCWWGG 1 cut(s) 449
Eco147I AGGCCT 2 cut(s) 857, 4128
Eco24I GRGCYC 2 cut(s) 320, 944
Eco31I GGTCTC 3 cut(s) 2483, 3468, 4227
Eco32I GATATC 2 cut(s) 1183, 3319
Eco47I GGWCC 1 cut(s) 485
Eco52I CGGCCG 1 cut(s) 135
Eco53kI GAGCTC 1 cut(s) 318
Eco57I CTGAAG 5 cut(s) 290, 1041, 1629, 3599, 4175
Eco72I CACGTG 1 cut(s) 905
Eco88I CYCGRG 2 cut(s) 3458, 3587
EcoICRI GAGCTC 1 cut(s) 318
EcoNI CCTNNNNNAGG 1 cut(s) 3062
EcoRI GAATTC 1 cut(s) 3961
EcoRII CCWGG 6 cut(s) 1391, 1446, 2129, 2493, 3012, 4217
EcoRV GATATC 2 cut(s) 1183, 3319
EcoT14I CCWWGG 1 cut(s) 449
EcoT22I ATGCAT 1 cut(s) 1443
EcoT38I GRGCYC 2 cut(s) 320, 944
ErhI CCWWGG 1 cut(s) 449
Esp3I CGTCTC 1 cut(s) 3965
FaqI GGGAC 5 cut(s) 833, 1377, 2095, 3405, 3606
FauI CCCGC 1 cut(s) 2328
FblI GTMKAC 4 cut(s) 96, 1119, 2413, 4017
FriOI GRGCYC 2 cut(s) 320, 944
FspBI CTAG 7 cut(s) 123, 203, 587, 1919, 2102, 2771, 2975
GlaI GCGC 1 cut(s) 3858
GsaI CCCAGC 1 cut(s) 3303
GsuI CTGGAG 6 cut(s) 131, 600, 711, 779, 2516, 3521
HaeII RGCGCY 1 cut(s) 3860
HaeIII GGCC 5 cut(s) 137, 636, 857, 3676, 4128
HapII CCGG 4 cut(s) 32, 3143, 3212, 3459
HgaI GACGC 3 cut(s) 90, 917, 1919
HhaI GCGC 1 cut(s) 3859
Hin1I GRCGYC 2 cut(s) 82, 909
Hin6I GCGC 1 cut(s) 3857
HinP1I GCGC 1 cut(s) 3857
HincII GTYRAC 2 cut(s) 97, 132
HindII GTYRAC 2 cut(s) 97, 132
HindIII AAGCTT 4 cut(s) 2579, 2627, 2833, 4100
HpaII CCGG 4 cut(s) 32, 3143, 3212, 3459
HphI GGTGA 8 cut(s) 1784, 1874, 2257, 2624, 3077, 3598, 3929, 4054
Hpy166II GTNNAC 9 cut(s) 97, 132, 1120, 1155, 1952, 2414, 2740, 3494, 4018
Hpy8I GTNNAC 9 cut(s) 97, 132, 1120, 1155, 1952, 2414, 2740, 3494, 4018
HpyCH4IV ACGT 7 cut(s) 128, 719, 904, 1105, 1366, 3031, 4075
HpySE526I ACGT 7 cut(s) 128, 719, 904, 1105, 1366, 3031, 4075
Hsp92I GRCGYC 2 cut(s) 82, 909
HspAI GCGC 1 cut(s) 3857
Kpn2I TCCGGA 2 cut(s) 3142, 3211
KpnI GGTACC 1 cut(s) 3071
LmnI GCTCC 7 cut(s) 315, 730, 742, 872, 939, 2875, 3990
MabI ACCWGGT 1 cut(s) 1446
MaeI CTAG 7 cut(s) 123, 203, 587, 1919, 2102, 2771, 2975
MaeII ACGT 7 cut(s) 128, 719, 904, 1105, 1366, 3031, 4075
MfeI CAATTG 7 cut(s) 1289, 1767, 1988, 2220, 2366, 3002, 3831
MflI RGATCY 3 cut(s) 3760, 3992, 4083
MhlI GDGCHC 2 cut(s) 320, 944
MlsI TGGCCA 1 cut(s) 636
MluNI TGGCCA 1 cut(s) 636
MmeI TCCRAC 2 cut(s) 850, 959
Mox20I TGGCCA 1 cut(s) 636
Mph1103I ATGCAT 1 cut(s) 1443
MroI TCCGGA 2 cut(s) 3142, 3211
MscI TGGCCA 1 cut(s) 636
MslI CAYNNNNRTG 4 cut(s) 1907, 2327, 3114, 3183
Msp20I TGGCCA 1 cut(s) 636
MspA1I CMGCKG 3 cut(s) 878, 3499, 3534
MspCI CTTAAG 2 cut(s) 41, 86
MspI CCGG 4 cut(s) 32, 3143, 3212, 3459
MspR9I CCNGG 9 cut(s) 32, 1393, 1448, 2131, 2495, 3014, 3459, 3460, 4219
MunI CAATTG 7 cut(s) 1289, 1767, 1988, 2220, 2366, 3002, 3831
Mva1269I GAATGC 1 cut(s) 23
MvaI CCWGG 6 cut(s) 1393, 1448, 2131, 2495, 3014, 4219
MvnI CGCG 1 cut(s) 1429
NciI CCSGG 3 cut(s) 32, 3459, 3460
NcoI CCATGG 1 cut(s) 449
NlaIV GGNNCC 4 cut(s) 941, 1916, 3069, 3521
NmeAIII GCCGAG 2 cut(s) 3684, 3702
NmuCI GTSAC 7 cut(s) 715, 895, 905, 1367, 1577, 1650, 1880
NruI TCGCGA 1 cut(s) 1429
NsiI ATGCAT 1 cut(s) 1443
NspI RCATGY 4 cut(s) 614, 1507, 2236, 2886
OliI CACNNNNGTG 2 cut(s) 3114, 3183
PagI TCATGA 1 cut(s) 2826
PaqCI CACCTGC 1 cut(s) 3958
PceI AGGCCT 2 cut(s) 857, 4128
PciI ACATGT 3 cut(s) 610, 2232, 2882
PctI GAATGC 1 cut(s) 23
PfoI TCCNGGA 1 cut(s) 3012
PmaCI CACGTG 1 cut(s) 905
PmlI CACGTG 1 cut(s) 905
Ppu21I YACGTR 1 cut(s) 905
PscI ACATGT 3 cut(s) 610, 2232, 2882
PshBI ATTAAT 3 cut(s) 3347, 3623, 4166
PsiI TTATAA 1 cut(s) 948
Psp124BI GAGCTC 1 cut(s) 320
Psp1406I AACGTT 1 cut(s) 128
Psp6I CCWGG 6 cut(s) 1391, 1446, 2129, 2493, 3012, 4217
PspCI CACGTG 1 cut(s) 905
PspFI CCCAGC 1 cut(s) 3299
PspGI CCWGG 6 cut(s) 1391, 1446, 2129, 2493, 3012, 4217
PspN4I GGNNCC 4 cut(s) 941, 1916, 3069, 3521
PspPI GGNCC 1 cut(s) 485
PsrI GAACNNNNNNTAC 2 cut(s) 2397, 2429
PstI CTGCAG 2 cut(s) 855, 1477
PstNI CAGNNNCTG 1 cut(s) 2595
PsuI RGATCY 3 cut(s) 3760, 3992, 4083
PvuII CAGCTG 3 cut(s) 878, 3499, 3534
RruI TCGCGA 1 cut(s) 1429
RsaI GTAC 8 cut(s) 74, 577, 1850, 1951, 2741, 2752, 3069, 4160
RsaNI GTAC 8 cut(s) 73, 576, 1849, 1950, 2740, 2751, 3068, 4159
RseI CAYNNNNRTG 4 cut(s) 1907, 2327, 3114, 3183
SacI GAGCTC 1 cut(s) 320
SalI GTCGAC 1 cut(s) 95
Sau96I GGNCC 1 cut(s) 485
ScaI AGTACT 1 cut(s) 74
ScrFI CCNGG 9 cut(s) 32, 1393, 1448, 2131, 2495, 3014, 3459, 3460, 4219
SduI GDGCHC 2 cut(s) 320, 944
SexAI ACCWGGT 1 cut(s) 1446
SfcI CTRYAG 2 cut(s) 851, 1473
SinI GGWCC 1 cut(s) 485
SmaI CCCGGG 1 cut(s) 3460
SmiMI CAYNNNNRTG 4 cut(s) 1907, 2327, 3114, 3183
SmlI CTYRAG 6 cut(s) 41, 86, 383, 2095, 2648, 3395
SmoI CTYRAG 6 cut(s) 41, 86, 383, 2095, 2648, 3395
SseBI AGGCCT 2 cut(s) 857, 4128
SsiI CCGC 8 cut(s) 53, 146, 274, 1325, 1624, 2335, 3487, 3953
SspI AATATT 1 cut(s) 3025
SspMI CTAG 7 cut(s) 123, 203, 587, 1919, 2102, 2771, 2975
SstI GAGCTC 1 cut(s) 320
StuI AGGCCT 2 cut(s) 857, 4128
StyD4I CCNGG 9 cut(s) 30, 1391, 1446, 2129, 2493, 3012, 3457, 3458, 4217
StyI CCWWGG 1 cut(s) 449
TaiI ACGT 7 cut(s) 131, 722, 907, 1108, 1369, 3034, 4078
TatI WGTACW 3 cut(s) 72, 1848, 2750
TauI GCSGC 1 cut(s) 3956
TscAI CASTG 7 cut(s) 73, 855, 1816, 1902, 2436, 3831, 4122
TseFI GTSAC 7 cut(s) 715, 895, 905, 1367, 1577, 1650, 1880
Tsp45I GTSAC 7 cut(s) 715, 895, 905, 1367, 1577, 1650, 1880
TspGWI ACGGA 1 cut(s) 3989
TspMI CCCGGG 1 cut(s) 3458
TspRI CASTG 7 cut(s) 73, 855, 1816, 1902, 2436, 3831, 4122
Vha464I CTTAAG 2 cut(s) 41, 86
VpaK11BI GGWCC 1 cut(s) 485
VspI ATTAAT 3 cut(s) 3347, 3623, 4166
XagI CCTNNNNNAGG 1 cut(s) 3062
XapI RAATTY 4 cut(s) 1274, 2291, 3334, 3961
XceI RCATGY 4 cut(s) 614, 1507, 2236, 2886
XmaI CCCGGG 1 cut(s) 3458
XmiI GTMKAC 4 cut(s) 96, 1119, 2413, 4017
XspI CTAG 7 cut(s) 123, 203, 587, 1919, 2102, 2771, 2975
ZrmI AGTACT 1 cut(s) 74
Zsp2I ATGCAT 1 cut(s) 1443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.