MD11G1044500.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000070
Physical Location & Seq
Reverse (-)
3911111 .. 3914039
2929 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1044500.v1.1.491

Sequence Viewer

Length: 2829 bp
ATGTCACTAGGAGAGGTCTTTATTTCTGGATTACTTCAAGTGTTACTTGAAAGGTTGACATCTCGTGAGACGCTGAGCTACTTTAGACGCCTACTTGGCGTTGGCAAAGAGCCGGACAAATGGAATTCAATGTTGTTTGCTATTCAAGCAGTACTGAATGACGCGGAGGAGAAGCAGTTGACGAGCCGAGCAGTGAAATTGTGGCTGGATGGTCTTAGAGACTTGGCTTATGATGTGGAAGACATATTGGACAAGTATTCAACCGAGATATTGCGACGCCAAATAAAGAAGAAACATTGGGCTACCACAAGCAAAATTACTGATCGGTTACGTGAGATAGTGGAACGGAAAAACCAACTTGGTTTAAATGAGGGGACGCCATCTACTAAGGAATGGCATGTGTCACCAAGTTCCTATCTACCAGAAGGACCTGTGATTGGAAGGGATACAGACAAGATGAAGATTATTGACTTGTTGGTGAAAGAAGCACCCCGTGTCGTCAACTTTCATGTAGTTGCCATTGTCGGTATGCCTGGAGTGGGAAAGACAACACTTGCAAGGCATGTATATGATGATGACGCGACAAAAGAGTTTAACCTAAAAGTGTGGGTATCTGTGTCGGATGACTTCAATCTTGAGAGAGTGACAAAGGCAATTCTTGAATCCGCCACATCTGGTCTTGCAAAGGAGTACAAGGAATTCAACCAGGTTCAAGAAAGTTTGAGTAAGGAGCTCGCAGGAAAAAAGTTTCTAATTTTTTTAGACGATGTTTGGAATACATGTGTCTATGATTTGTGGATAAAATTGCAGTCCCCCTTTCGTGTTGGAGCATTAGGAAGTAAGATAGTTGTGACAACACGTGATGCAAATGTTGCAAACATGATGGGATCCACTGTTTATCAATTGGGTGGCATAACAGACGATCAATGTTGGGAAGTCTTTGAGCAGCATTCGCTCTTGGACATTACCAATAAAAGGCCGCAAAAGTTTGAGTTGATAAAGGAGAAAATTGTTGCAAAATGCCGCGGATTGCCATTGGCTGCAAGGACTCTTGGAGGTCTTCTACGTTGTAAACAAGTGGAAGAATGGGAGGCAATCTTAAACAACAAGATGTGGAGTCTATCAGACGATAGTGGCATTCTCCCGGTATTAAAATTGAGCTATCACTATCTCCCTTCGAGTTTGAAACGGTGCTTCGCCTATTGTTCCCTACTTCCAAATGACTATGAGTTCGGGGAGAAGCAGTTGATCCTTCTGTGGATGGCAGAAGGTTTGATTCAACAAAGAGTAGAGGACAAGAAACAATTGGAAGAAGTAGGCAGTGACTATTTTCAAGAGTTATTGTCCAGGTCATTATTTCAAAAGGCAAGCAAAAGCAATGATAAGTATGTAATGCATGACCTTATTGGTGAGTTGGCGCGGTGGGCTGCAGCTGAAATATGCTTTTCATTGGAGGACAAGGAAAATTGTGACTTGCATCCAATGGCTCGTCATATGTCTTATGTCAGTGGTAGGTTTGATGGGGTTAAAAAATTTGAGGCCATTTCCAAAGTTAAACATTTGAGGACATTCCTGCCATTTGCACAAGATTTAATTCGGCTTTCACAATATAATCGGAACTATCTAACTCGTCGGCTTACATTTGATCTATTGCCAGAACTGCGAATCTTACGGGTGCTTTCTTTGAATGGCTATGAAATAACCGAGCTGCCCAATTCCATTGGTAAATTGTGGAATCTACGGTATCTGGACCTTTCTTATACAAAGATAAAGAGTTTGCCTCCATCAACAACCACTCTTTGCAATCTGCAAACATTGTTATTAAAAGGTTGTTCTAAATTGAAGGCATTGCCCTCAAACTTGAGTAATCTAATTAATTTGCGGCATCTCAACAATTCCAGTGGATTGGAAGGAATGCCTCCGCATATAGGTCGATTGACGAATCTGCAATCCTTGTCGTATTTTGTGGTAGGCAAAGGTAGTGATCAGTCTGGGATAAGAGAGATAGGGTCCCTACTGCATCTCCGAGGGACATTGTGCCTCTCAAGATTGGAGAATGTGGTTGATGCTAAGGATGCTAGTGAGGCCAAGTTAATAGACAAGGAGAGGCTTGATTCATTACAGCTGGAATGGTCTAATTTGAGCGGATCGAGGGAAATGGAGTTGGGTGTGCTTGACATGTTACAACCTCCTAAAAATCTCAAAGAGCTCACCATTGAAGGTTATGCTGGATTGAAATTTTCATCGTGGATTGGAGATCCTCTGTTCTCTAACATGGTCCGTGTAAGCTTATACAATTGTGCAAGTTGTCATCTCTTGCCACCAGTTGGACAGTTGCCTTGCCTTAAAGAACTTTATATCGGGGGAATGACTGCCGTGGAAATCATTGGTCCTGAATTCTACGGAGAGGGTAGTTTGCCTTTTCGAGTATTAAAGATTCTCAAGTTTTCGGAGATGGAGCAGTGGGAGAAATGGCTGCCTTTCGATCAGGATAAGGGAAGTGGTGTTTTCCCTAGCCTAAAAATGCTTTTAGTCCAAGGCTGCCCTAAATTGGAGGGTGAGTTGCCGAAGAAACTTAATTCGTTATCAAAGCTTGAGATTTATAGGTGCAAGGAATTGGTGGTGGAAATTGCCAATTATGAACACGCGAGTGAAGTAAGCATCCATGGTTGCAAAGCGCTTGTGCATACTAGCGCAGAGGTTGACTTTAAGTTATTAGAGACCATGAGGCTTTCAAATATTTTGGAGCCGCGGTTGGGAGCAAGGGGACTTACGAAAGGATTGGGCAAGATTAAGGAGTTGAAGATTAGTGATGTTTCCCACACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

943

Amino Acids

106.73

Weight (kDa)

8.23

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 99 6.6e-22 Rx N-terminal domain
NB-ARC PF00931 150 - 317 2.4e-32 NB-ARC domain
WHD_DRP PF23559 405 - 473 6.8e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 551 - 845 1e-26 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 666 - 790 2.9e-36 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 2327
AccBSI CCGCTC 1 cut(s) 2145
AccII CGCG 6 cut(s) 164, 581, 1026, 1420, 2648, 2752
AclWI GGATC 5 cut(s) 882, 895, 1243, 2155, 2252
AcsI RAATTY 5 cut(s) 124, 698, 1532, 2237, 2396
AcvI CACGTG 1 cut(s) 860
AcyI GRCGYC 3 cut(s) 88, 277, 377
AdeI CACNNNGTG 1 cut(s) 494
AfaI GTAC 2 cut(s) 153, 692
AfeI AGCGCT 1 cut(s) 2679
AfiI CCNNNNNNNGG 7 cut(s) 437, 539, 975, 1928, 2327, 2551, 2756
AflIII ACRYGT 3 cut(s) 779, 857, 2178
AjnI CCWGG 3 cut(s) 532, 705, 1346
AleI CACNNNNGTG 1 cut(s) 2649
AluBI AGCT 9 cut(s) 78, 733, 1161, 1433, 1708, 2125, 2209, 2289, 2593
AluI AGCT 9 cut(s) 78, 733, 1161, 1433, 1708, 2125, 2209, 2289, 2593
Alw21I GWGCWC 2 cut(s) 735, 2211
Alw26I GTCTC 3 cut(s) 62, 213, 2714
AlwI GGATC 5 cut(s) 882, 895, 1243, 2155, 2252
Aor51HI AGCGCT 1 cut(s) 2679
AoxI GGCC 3 cut(s) 977, 1539, 2085
ApeKI GCWGC 7 cut(s) 946, 1040, 1427, 1430, 1708, 2476, 2541
ApoI RAATTY 5 cut(s) 124, 698, 1532, 2237, 2396
ArsI GACNNNNNNTTYG 2 cut(s) 1214, 1246
AseI ATTAAT 1 cut(s) 1875
AspLEI GCGC 3 cut(s) 1420, 2680, 2696
AspS9I GGNCC 5 cut(s) 428, 1750, 2010, 2278, 2390
AsuC2I CCSGG 1 cut(s) 1145
AsuHPI GGTGA 5 cut(s) 396, 490, 1421, 2203, 2570
AvaII GGWCC 5 cut(s) 428, 1750, 2010, 2278, 2390
BamHI GGATCC 1 cut(s) 887
BanII GRGCYC 2 cut(s) 735, 2211
BauI CACGAG 1 cut(s) 63
BbrPI CACGTG 1 cut(s) 860
BbsI GAAGAC 2 cut(s) 246, 1052
Bbv12I GWGCWC 2 cut(s) 735, 2211
BbvI GCAGC 7 cut(s) 958, 1027, 1414, 1442, 1695, 2463, 2528
BccI CCATC 7 cut(s) 203, 388, 877, 1255, 1514, 1792, 2449
BceAI ACGGC 1 cut(s) 2360
BcgI CGANNNNNNTGC 2 cut(s) 1913, 1947
BciT130I CCWGG 3 cut(s) 534, 707, 1348
BciVI GTATCC 1 cut(s) 439
BclI TGATCA 1 cut(s) 1984
BcnI CCSGG 1 cut(s) 1145
BcoDI GTCTC 3 cut(s) 62, 213, 2714
BfaI CTAG 4 cut(s) 8, 2079, 2514, 2691
BfmI CTRYAG 1 cut(s) 1428
BfoI RGCGCY 1 cut(s) 2681
BfuI GTATCC 1 cut(s) 439
BglI GCCNNNNNGGC 1 cut(s) 96
BlpI GCTNAGC 1 cut(s) 74
BmcAI AGTACT 1 cut(s) 153
Bme1390I CCNGG 4 cut(s) 534, 707, 1145, 1348
Bme18I GGWCC 5 cut(s) 428, 1750, 2010, 2278, 2390
BmgT120I GGNCC 5 cut(s) 428, 1750, 2010, 2278, 2390
BmiI GGNNCC 4 cut(s) 889, 2011, 2012, 2748
BmrFI CCNGG 4 cut(s) 534, 707, 1145, 1348
BmsI GCATC 7 cut(s) 853, 1486, 1894, 2029, 2056, 2065, 2670
BpiI GAAGAC 2 cut(s) 246, 1052
BplI GAGNNNNNCTC 2 cut(s) 1765, 1797
BpmI CTGGAG 1 cut(s) 555
Bpu10I CCTNAGC 1 cut(s) 2070
Bpu1102I GCTNAGC 1 cut(s) 74
BpuEI CTTGAG 5 cut(s) 656, 1882, 2029, 2426, 2615
BpuMI CCSGG 1 cut(s) 1145
BsaAI YACGTR 2 cut(s) 332, 860
BsaHI GRCGYC 3 cut(s) 88, 277, 377
BsaI GGTCTC 1 cut(s) 2714
BsaJI CCNNGG 6 cut(s) 1024, 2026, 2376, 2536, 2665, 2750
Bsc4I CCNNNNNNNGG 7 cut(s) 437, 539, 975, 1928, 2327, 2551, 2756
Bse1I ACTGG 2 cut(s) 1899, 2324
Bse3DI GCAATG 2 cut(s) 1384, 1847
BseBI CCWGG 3 cut(s) 534, 707, 1348
BseDI CCNNGG 6 cut(s) 1024, 2026, 2376, 2536, 2665, 2750
BseGI GGATG 6 cut(s) 214, 628, 1266, 1477, 2080, 2661
BseLI CCNNNNNNNGG 7 cut(s) 437, 539, 975, 1928, 2327, 2551, 2756
BseMI GCAATG 2 cut(s) 1384, 1847
BseMII CTCAG 1 cut(s) 65
BseNI ACTGG 2 cut(s) 1899, 2324
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 7 cut(s) 958, 1027, 1414, 1442, 1695, 2463, 2528
Bsh1236I CGCG 6 cut(s) 164, 581, 1026, 1420, 2648, 2752
BshFI GGCC 3 cut(s) 979, 1541, 2087
BsiHKAI GWGCWC 2 cut(s) 735, 2211
BsiSI CCGG 2 cut(s) 113, 1145
BslFI GGGAC 5 cut(s) 388, 796, 1996, 2044, 2781
BslI CCNNNNNNNGG 7 cut(s) 437, 539, 975, 1928, 2327, 2551, 2756
BsmAI GTCTC 3 cut(s) 62, 213, 2714
BsmBI CGTCTC 1 cut(s) 62
BsmFI GGGAC 5 cut(s) 388, 796, 1996, 2044, 2781
BsmI GAATGC 3 cut(s) 949, 1137, 1920
BsnI GGCC 3 cut(s) 979, 1541, 2087
Bso31I GGTCTC 1 cut(s) 2714
Bsp1286I GDGCHC 2 cut(s) 735, 2211
Bsp143I GATC 9 cut(s) 322, 887, 922, 1248, 1645, 1984, 2147, 2257, 2485
Bsp1720I GCTNAGC 1 cut(s) 74
Bsp19I CCATGG 1 cut(s) 2665
BspANI GGCC 3 cut(s) 979, 1541, 2087
BspCNI CTCAG 1 cut(s) 66
BspFNI CGCG 6 cut(s) 164, 581, 1026, 1420, 2648, 2752
BspLI GGNNCC 4 cut(s) 889, 2011, 2012, 2748
BspMAI CTGCAG 1 cut(s) 1432
BspPI GGATC 5 cut(s) 882, 895, 1243, 2155, 2252
BspTNI GGTCTC 1 cut(s) 2714
BsrBI CCGCTC 1 cut(s) 2145
BsrDI GCAATG 2 cut(s) 1384, 1847
BsrI ACTGG 2 cut(s) 1899, 2324
BssECI CCNNGG 6 cut(s) 1024, 2026, 2376, 2536, 2665, 2750
BssMI GATC 9 cut(s) 322, 887, 922, 1248, 1645, 1984, 2147, 2257, 2485
BssNI GRCGYC 3 cut(s) 88, 277, 377
BssSI CACGAG 1 cut(s) 63
BssT1I CCWWGG 2 cut(s) 2536, 2665
Bst2BI CACGAG 1 cut(s) 63
Bst2UI CCWGG 3 cut(s) 534, 707, 1348
Bst4CI ACNGT 4 cut(s) 895, 1191, 1743, 2334
BstACI GRCGYC 3 cut(s) 88, 277, 377
BstAPI GCANNNNNTGC 1 cut(s) 872
BstBAI YACGTR 2 cut(s) 332, 860
BstC8I GCNNGC 2 cut(s) 735, 1369
BstDEI CTNAG 4 cut(s) 74, 215, 387, 2070
BstDSI CCRYGG 4 cut(s) 1024, 2376, 2665, 2750
BstF5I GGATG 6 cut(s) 214, 628, 1266, 1477, 2080, 2661
BstFNI CGCG 6 cut(s) 164, 581, 1026, 1420, 2648, 2752
BstH2I RGCGCY 1 cut(s) 2681
BstHHI GCGC 3 cut(s) 1420, 2680, 2696
BstKTI GATC 9 cut(s) 325, 890, 925, 1251, 1648, 1987, 2150, 2260, 2488
BstMAI GTCTC 3 cut(s) 62, 213, 2714
BstMBI GATC 9 cut(s) 322, 887, 922, 1248, 1645, 1984, 2147, 2257, 2485
BstMWI GCNNNNNNNGC 8 cut(s) 96, 146, 872, 952, 1424, 1660, 2075, 2084
BstNI CCWGG 3 cut(s) 534, 707, 1348
BstNSI RCATGY 4 cut(s) 401, 566, 783, 2182
BstSCI CCNGG 4 cut(s) 532, 705, 1143, 1346
BstSFI CTRYAG 1 cut(s) 1428
BstUI CGCG 6 cut(s) 164, 581, 1026, 1420, 2648, 2752
BstV1I GCAGC 7 cut(s) 958, 1027, 1414, 1442, 1695, 2463, 2528
BstV2I GAAGAC 2 cut(s) 246, 1052
BstX2I RGATCY 2 cut(s) 887, 2257
BstXI CCANNNNNNTGG 1 cut(s) 1906
BstYI RGATCY 2 cut(s) 887, 2257
BsuI GTATCC 1 cut(s) 439
BsuRI GGCC 3 cut(s) 979, 1541, 2087
BtgI CCRYGG 4 cut(s) 1024, 2376, 2665, 2750
BtsCI GGATG 6 cut(s) 214, 628, 1266, 1477, 2080, 2661
BtsI GCAGTG 3 cut(s) 198, 1327, 2468
BtsIMutI CAGTG 6 cut(s) 198, 891, 1327, 1513, 1906, 2468
Cac8I GCNNGC 2 cut(s) 735, 1369
CfoI GCGC 3 cut(s) 1420, 2680, 2696
Cfr13I GGNCC 5 cut(s) 428, 1750, 2010, 2278, 2390
Cfr42I CCGCGG 2 cut(s) 1027, 2753
CseI GACGC 6 cut(s) 79, 96, 170, 285, 385, 587
CsiI ACCWGGT 1 cut(s) 705
Csp6I GTAC 2 cut(s) 152, 691
CspCI CAANNNNNGTGG 2 cut(s) 1882, 1917
CviQI GTAC 2 cut(s) 152, 691
DdeI CTNAG 4 cut(s) 74, 215, 387, 2070
DpnI GATC 9 cut(s) 324, 889, 924, 1250, 1647, 1986, 2149, 2259, 2487
DpnII GATC 9 cut(s) 322, 887, 922, 1248, 1645, 1984, 2147, 2257, 2485
DraI TTTAAA 1 cut(s) 366
DraIII CACNNNGTG 1 cut(s) 494
EciI GGCGGA 1 cut(s) 655
Ecl136II GAGCTC 2 cut(s) 733, 2209
Eco130I CCWWGG 2 cut(s) 2536, 2665
Eco24I GRGCYC 2 cut(s) 735, 2211
Eco31I GGTCTC 1 cut(s) 2714
Eco47I GGWCC 5 cut(s) 428, 1750, 2010, 2278, 2390
Eco47III AGCGCT 1 cut(s) 2679
Eco53kI GAGCTC 2 cut(s) 733, 2209
Eco72I CACGTG 1 cut(s) 860
EcoICRI GAGCTC 2 cut(s) 733, 2209
EcoO109I RGGNCCY 2 cut(s) 428, 2010
EcoRI GAATTC 3 cut(s) 124, 698, 2396
EcoRII CCWGG 3 cut(s) 532, 705, 1346
EcoT14I CCWWGG 2 cut(s) 2536, 2665
EcoT22I ATGCAT 1 cut(s) 1398
EcoT38I GRGCYC 2 cut(s) 735, 2211
ErhI CCWWGG 2 cut(s) 2536, 2665
Esp3I CGTCTC 1 cut(s) 62
FalI AAGNNNNNCTT 2 cut(s) 30, 62
FaqI GGGAC 5 cut(s) 388, 796, 1996, 2044, 2781
FauNDI CATATG 1 cut(s) 1494
FbaI TGATCA 1 cut(s) 1984
FokI GGATG 6 cut(s) 221, 635, 1273, 1464, 2087, 2648
FriOI GRGCYC 2 cut(s) 735, 2211
FspBI CTAG 4 cut(s) 8, 2079, 2514, 2691
GlaI GCGC 3 cut(s) 1419, 2679, 2695
GsuI CTGGAG 1 cut(s) 555
HaeII RGCGCY 1 cut(s) 2681
HaeIII GGCC 3 cut(s) 979, 1541, 2087
HapII CCGG 2 cut(s) 113, 1145
HgaI GACGC 6 cut(s) 79, 96, 170, 285, 385, 587
HhaI GCGC 3 cut(s) 1420, 2680, 2696
Hin1I GRCGYC 3 cut(s) 88, 277, 377
Hin6I GCGC 3 cut(s) 1418, 2678, 2694
HinP1I GCGC 3 cut(s) 1418, 2678, 2694
HincII GTYRAC 4 cut(s) 57, 180, 502, 2704
HindII GTYRAC 4 cut(s) 57, 180, 502, 2704
HindIII AAGCTT 2 cut(s) 2287, 2591
HinfI GANTC 9 cut(s) 662, 1048, 1117, 1276, 1665, 1735, 1942, 2114, 2437
HpaII CCGG 2 cut(s) 113, 1145
HphI GGTGA 5 cut(s) 396, 490, 1421, 2203, 2570
Hpy166II GTNNAC 5 cut(s) 57, 180, 502, 1073, 2704
Hpy188I TCNGA 5 cut(s) 622, 1126, 1617, 2027, 2452
Hpy8I GTNNAC 5 cut(s) 57, 180, 502, 1073, 2704
Hpy99I CGWCG 2 cut(s) 279, 1635
HpyAV CCTTC 8 cut(s) 419, 435, 1185, 1262, 1262, 1837, 1904, 2213
HpyCH4III ACNGT 4 cut(s) 895, 1191, 1743, 2334
HpyCH4IV ACGT 3 cut(s) 331, 859, 1066
HpyF10VI GCNNNNNNNGC 8 cut(s) 96, 146, 872, 952, 1424, 1660, 2075, 2084
HpyF3I CTNAG 4 cut(s) 74, 215, 387, 2070
HpySE526I ACGT 3 cut(s) 331, 859, 1066
Hsp92I GRCGYC 3 cut(s) 88, 277, 377
HspAI GCGC 3 cut(s) 1418, 2678, 2694
KflI GGGWCCC 1 cut(s) 2010
Ksp22I TGATCA 1 cut(s) 1984
KspI CCGCGG 2 cut(s) 1027, 2753
Kzo9I GATC 9 cut(s) 322, 887, 922, 1248, 1645, 1984, 2147, 2257, 2485
LmnI GCTCC 5 cut(s) 730, 827, 2458, 2746, 2759
Lsp1109I GCAGC 7 cut(s) 958, 1027, 1414, 1442, 1695, 2463, 2528
LweI GCATC 7 cut(s) 853, 1486, 1894, 2029, 2056, 2065, 2670
MabI ACCWGGT 1 cut(s) 705
MaeI CTAG 4 cut(s) 8, 2079, 2514, 2691
MaeII ACGT 3 cut(s) 331, 859, 1066
MaeIII GTNAC 9 cut(s) 3, 42, 327, 402, 643, 850, 1322, 1469, 2181
MalI GATC 9 cut(s) 324, 889, 924, 1250, 1647, 1986, 2149, 2259, 2487
MbiI CCGCTC 1 cut(s) 2145
MboI GATC 9 cut(s) 322, 887, 922, 1248, 1645, 1984, 2147, 2257, 2485
MboII GAAGA 8 cut(s) 251, 301, 472, 1052, 1094, 1322, 2581, 2815
MfeI CAATTG 3 cut(s) 902, 1304, 2296
MflI RGATCY 2 cut(s) 887, 2257
MhlI GDGCHC 2 cut(s) 735, 2211
MlyI GAGTC 2 cut(s) 1042, 1126
MmeI TCCRAC 3 cut(s) 600, 805, 2308
Mph1103I ATGCAT 1 cut(s) 1398
MslI CAYNNNNRTG 2 cut(s) 567, 2649
MspA1I CMGCKG 4 cut(s) 1026, 1433, 2125, 2752
MspI CCGG 2 cut(s) 113, 1145
MspR9I CCNGG 4 cut(s) 534, 707, 1145, 1348
MunI CAATTG 3 cut(s) 902, 1304, 2296
Mva1269I GAATGC 3 cut(s) 949, 1137, 1920
MvaI CCWGG 3 cut(s) 534, 707, 1348
MvnI CGCG 6 cut(s) 164, 581, 1026, 1420, 2648, 2752
MwoI GCNNNNNNNGC 8 cut(s) 96, 146, 872, 952, 1424, 1660, 2075, 2084
NciI CCSGG 1 cut(s) 1145
NcoI CCATGG 1 cut(s) 2665
NdeI CATATG 1 cut(s) 1494
NdeII GATC 9 cut(s) 322, 887, 922, 1248, 1645, 1984, 2147, 2257, 2485
NlaIV GGNNCC 4 cut(s) 889, 2011, 2012, 2748
NmeAIII GCCGAG 1 cut(s) 212
NmuCI GTSAC 6 cut(s) 3, 402, 643, 850, 1322, 1469
NsiI ATGCAT 1 cut(s) 1398
NspI RCATGY 4 cut(s) 401, 566, 783, 2182
OliI CACNNNNGTG 1 cut(s) 2649
PciI ACATGT 2 cut(s) 779, 2178
PctI GAATGC 3 cut(s) 949, 1137, 1920
PfeI GAWTC 7 cut(s) 662, 1276, 1665, 1735, 1942, 2114, 2437
PflMI CCANNNNNTGG 1 cut(s) 2327
PleI GAGTC 2 cut(s) 1042, 1125
PmaCI CACGTG 1 cut(s) 860
PmlI CACGTG 1 cut(s) 860
PpsI GAGTC 2 cut(s) 1042, 1125
Ppu21I YACGTR 2 cut(s) 332, 860
PpuMI RGGWCCY 2 cut(s) 428, 2010
PscI ACATGT 2 cut(s) 779, 2178
PshBI ATTAAT 1 cut(s) 1875
Psp124BI GAGCTC 2 cut(s) 735, 2211
Psp5II RGGWCCY 2 cut(s) 428, 2010
Psp6I CCWGG 3 cut(s) 532, 705, 1346
PspCI CACGTG 1 cut(s) 860
PspGI CCWGG 3 cut(s) 532, 705, 1346
PspN4I GGNNCC 4 cut(s) 889, 2011, 2012, 2748
PspPI GGNCC 5 cut(s) 428, 1750, 2010, 2278, 2390
PspPPI RGGWCCY 2 cut(s) 428, 2010
PstI CTGCAG 1 cut(s) 1432
PsuI RGATCY 2 cut(s) 887, 2257
PvuII CAGCTG 2 cut(s) 1433, 2125
RsaI GTAC 2 cut(s) 153, 692
RsaNI GTAC 2 cut(s) 152, 691
RseI CAYNNNNRTG 2 cut(s) 567, 2649
SacI GAGCTC 2 cut(s) 735, 2211
SacII CCGCGG 2 cut(s) 1027, 2753
Sau3AI GATC 9 cut(s) 322, 887, 922, 1248, 1645, 1984, 2147, 2257, 2485
Sau96I GGNCC 5 cut(s) 428, 1750, 2010, 2278, 2390
ScaI AGTACT 1 cut(s) 153
SchI GAGTC 2 cut(s) 1042, 1126
ScrFI CCNGG 4 cut(s) 534, 707, 1145, 1348
SduI GDGCHC 2 cut(s) 735, 2211
SexAI ACCWGGT 1 cut(s) 705
SfaNI GCATC 7 cut(s) 853, 1486, 1894, 2029, 2056, 2065, 2670
SfcI CTRYAG 1 cut(s) 1428
Sfr303I CCGCGG 2 cut(s) 1027, 2753
SgrBI CCGCGG 2 cut(s) 1027, 2753
SinI GGWCC 5 cut(s) 428, 1750, 2010, 2278, 2390
SmiMI CAYNNNNRTG 2 cut(s) 567, 2649
SmlI CTYRAG 5 cut(s) 635, 1861, 2044, 2441, 2594
SmoI CTYRAG 5 cut(s) 635, 1861, 2044, 2441, 2594
SspI AATATT 1 cut(s) 2740
SspMI CTAG 4 cut(s) 8, 2079, 2514, 2691
SstI GAGCTC 2 cut(s) 735, 2211
StyD4I CCNGG 4 cut(s) 532, 705, 1143, 1346
StyI CCWWGG 2 cut(s) 2536, 2665
TaaI ACNGT 4 cut(s) 895, 1191, 1743, 2334
TaiI ACGT 3 cut(s) 334, 862, 1069
TaqI TCGA 5 cut(s) 1178, 1933, 2150, 2425, 2484
TatI WGTACW 2 cut(s) 151, 690
TauI GCSGC 4 cut(s) 982, 1026, 1885, 2752
TfiI GAWTC 7 cut(s) 662, 1276, 1665, 1735, 1942, 2114, 2437
TscAI CASTG 6 cut(s) 198, 898, 1327, 1513, 1906, 2468
TseFI GTSAC 6 cut(s) 3, 402, 643, 850, 1322, 1469
TseI GCWGC 7 cut(s) 946, 1040, 1427, 1430, 1708, 2476, 2541
Tsp45I GTSAC 6 cut(s) 3, 402, 643, 850, 1322, 1469
TspDTI ATGAA 7 cut(s) 473, 497, 1437, 1710, 2106, 2232, 2655
TspGWI ACGGA 3 cut(s) 361, 2270, 2418
TspRI CASTG 6 cut(s) 198, 898, 1327, 1513, 1906, 2468
Van91I CCANNNNNTGG 1 cut(s) 2327
VpaK11BI GGWCC 5 cut(s) 428, 1750, 2010, 2278, 2390
VspI ATTAAT 1 cut(s) 1875
XapI RAATTY 5 cut(s) 124, 698, 1532, 2237, 2396
XceI RCATGY 4 cut(s) 401, 566, 783, 2182
XspI CTAG 4 cut(s) 8, 2079, 2514, 2691
ZrmI AGTACT 1 cut(s) 153
Zsp2I ATGCAT 1 cut(s) 1398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.