Prupe.2G022400_v2.0.a1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000070
Physical Location & Seq
Reverse (-)
2184323 .. 2189899
5577 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G022400.3

Sequence Viewer

Length: 4224 bp
ATGGGAGAGGCTTATCTTGTGGCATTCCTTCAAGTGCTGGTCGACAAGTTGGCGCGTCGTGAGGTGTTCAAGTACTTTGGACTCATAAAGGGCGTCGACCAAAAGCTGCAGAAATGGACTGCCACCTTGTCTGCAATTGGAGCGGTTCTGAATGAGGCGGAGGAGAGGCAACTAATCACCGAGAGCAAGCCGCTGAAGCTTTGGCTGGATGATCTCAGGGACTTAGCTTATGACTTGGAAGACGTATTGGACAAATATGCTACTAAAATGCTCAAACGGGAGGTAGAGCATTATACCGCAAGAAGGGTCTGGAACTCAGTTCCTAATGGTGTTTTCAACTACAAAATGAACTCAGAAATACAGAAGATTACTGAGCGTTTACAAGGGATATCTCAGCGAAAAGACCAGCTTAGTTTGAATATCATTACTGGGACGACGTCGTCCAGTAAGGCACGTCAAAATTTACCTCCCAGTACATGTCAACCAGATGGACCTGTGATTGGAAGGGAGGAAGACAAAAGGCAGGTCGTGGAATTTCTGTCGAAACAAGAGCGCAATGCCGTCAATTTCGATGTAGTTGCCATTGTTGGTATGGCTGGAGTCGGAAAGACAACACTTGCTGCACAAGTGTTCAATGAAATTGATGCAACCGAGCAATTTAAACCAACTGCTTGGGTATGCGTGTCTGATGACTTCAACCTAGAAAGAGTGACAAAGCAGATTCTCGGAGCAGTCACATCCGGACAGTGTCCTACAGAAGATTTCAATCAGGTTCAACAACATCTGCATAAGGAATTGGCAGGGAAGAAGTTTTTAATTGTTTTAGATGATGTTTGGGGCACCTGTAGCTATGGTCTATGGATGAAACTGCAGTCCCCCTTTCGCGATGGAGCAGCAGGAAGCAAGATAATTGTGACAACGCGTGATGCAGAAGTTTCGAAAATGATGGGAGCTGGCACTCTAGTTCACAATTTGGAGCCTATGTCAAATGACGTTTGTTTTGAAGTATTTGAGCAGCATGCATTCAGGAATGTTAACAGAGATATACCACCAAATTTCGAGTCACTCAAGGAGAAAATTGTTGCAAGATGCAGTGGATTGCCTTTGGCTGCTAGGACTCTTGGCGGCCTTTTACTTAGTAAAAAGATGAATGAATGGGAGGAAATATTGAACAACAAATTATGGAGTCTATCAAATGAGTGTGACATACTTCCGGTATTAAGACTAAGCTATCACTATCTTCCTTCACATTTGAAAAGATGCTTTGCCTATTGTTCGATATTTCCAAATGACTATGAATTTTGTGAGCAGCAATTGATTCTTCTGTGGATGGCAGAGGGTTTGATTCAACCACAACCAGAACATAATAAGCAAATGGAAGATTTAGGAACTGACTATTTTCAGGATCTCTTATCGAGGTCATTATTTCAGAAATCAAGCAAAAACAATTCAAAGTATGTAATGCATGACCTCATTGTTGGTTTGGCACAGTGGGCAGCAGGAGATATTTGTTTTAGATTGGAGGATAAGCAGAATAGCGATCATGTACAACTTGGATGTTTTACGAAGGCCCGCCATGCGTCCTACATTTCTGGTTGCTATGATGTGGTTAAAAGATTTGAGGCATTTTCTGAAGTGAAACATTTCCGAACTTTTCTACCACTTTCAATTTCAGAGTATTATGAATCGAGGTTTCTGAGTCGTAAGGTTACTTTTGATTTATTGCCAAAATTGCAATACTTGCGGGTGCTTTCTCTCAATGGCTATCAAGTGACTGAGCTGCCAAATTCAATTGGTAAATTGAAGTATCTGCGGCATCTTGATCTTTCTTACACACACATAACAAGTTTGCCTGAATCAACAACCACTCTTTTCAACTTACAGACACTGATATTGAAAGGTTGTCGTTATTTGAAGGCACTACCCATAAACTTGAGGAATCTAGTGAATCTGCGTCATCTCAATAACTCAGATGTATATTCACTGAAAGCAATGCCTCCGCAACTAGGTCGGTTGACAAATCTACAATCTTTGCCTAAATTTGTGGTGGGTAAAGGTAGTGATGAATCAGGGATAAGAGAGATAGGATCCCTGTCCCATCTCCGAAGGACATTATCGCTCTCAAGGTTGGAGAATGTGATCGATGCTGAGGATGCACGGAAGGCCGGCTTAAAATCCAAGGAGAGAGTAGATGAATTGGTGCTAGAATGGACGGATAACACACAAGAAACGCAATTAGGCGTGCTTGACAGATTAGAACCTCATAGAAAGCTTGAAAAACTCATCATCAGGGGTTATGCTGGATTAGAATTTTCAACATGGATTGGAGATCGTTTATTCTCCACTATGGTGCATGTACGCTTAGACAAATGTAAAAATTGTCAAATCTTGCCACCACTTGGGCAACTGCCTTTGCTCAAAGAACTTTATATTACAGGAATGGCGGCAGTGAAAAGTGTCGGTCCTGAGTTTTATGGAGAGAGTAGCTTGCCTTTTCCAGTACTGGAGACTCTTGAGTTTTCAGATATGCACAACTGGAAGAAATGGCTTCCTTTTGTGGGGGATCGGGGAATTGGTGTTTTCCCTTGCTTGAAATTGCTCTCAATCACAAATTGTCCTCAATTGGAGGGTAAGGTGCCCGAGAACTTTGATTCATTAGAAACACTTAAAATTATTAAATGCGAGGAATTGGTGATTTCAATTTCCAACTATAAACAAATTGGTCGATTATGGATAGATGGCTGCAAAGCGGTGGTGGAGACAAGTGGCGTTGAGTTTGAGTTATTAAATTCCCTGGGGCTTTCAAATATTTCAGAGGTGAGGTTCCAAACAGGGGAATTCGCCAAAGGATTAAGGAAGGTTGCATATTTGAGGATTGGCGGATGTGAGGAGCTGACATCTTCACTGAAGAATGAGGATAGAGTATTGCAACACTTGATTTCTCTTGATCGTTTGGTTATTTCAGGCAACTCTTCCCTCCTTGAAAAGCTGGGAAAAGAAGCAGAGGAGTTGCTGCAATTGCAAATATTGACTTCCAAGCTTAAATATCTAGAATTATACAAGTGTGCAAGTCTTTCGAAGGTACCAGAAGGGTTGCATCACCTAACGGCTCTTCAAGACCTTCAAATAATTGGATGCTCAAGTCTGGTTTCTTTTCCAGATGTTGGTCTGCCTCCTTCCCTTGAAGTCATAAGGATTTGGGAGTGTGATTCGTTGCTGTATTTTGCAAAATGCCAGATTCCCCCAAATCTAAGAAGAATAGAGATACAGCGGTGTAAAAGTTTGAAATCATTAGTAGAGAAAGAGGAGGATAGTTCTTCGTCTTCCTCTTCTTCTCATATTTCTCTTGAGCACTTGGAAATATGGACATGTGAATCTCTAAAGTCGTTATCATTGAGAGCCCAGTTGTTTCCCAAGGCGCTTAAAAGCCTTCACATATACGATTGTGGAGAGCTGCAGTTAATAACATCCGATGGGTTCTCCCACGACAACACTAATTATTGTCTTGAATATATTACGATCTGGAGTTGCCGAAATTTCAAATCGTTACCGGAGGGCCTATGCTACCTCACCAACCTTCAAACTTTAGAGATTTATCGTTGTGGGAGTCTGGTTTCCATCCCGAGCCTGAGTGGTGAGGGTTTGCCTTCGCCAACAACAACAGCGGCCTCCAGCCTGAAAGAAATCTACATCGAAAATTGCAACAAATTGGAGATGTTACCGGACATGTGCAATCTCAACTGTCTTCAGGAATTGAATATTGATTACGGTGAAGGTTTAAATTTCACTTCCTTTCCCCCCAACCTAACATCACTTACAATCCGCAGAATCAAGAATTGTAAGCCGCTGTGGGAGTTGTTGCACAGGCTCACCTCTCTTACGAAGTTGTGGGTCGGTGGCGAAGACCCAAGTGTGGTGTCATTTCCACCCGACAGTTACAGGGAGATGCTGCTCCCCGAATCTCTCACTAATCTCTCCATTGTGGGCTTCCCAAATCTGAAGAAACTGAGCAGCAAGGGCTTTCAATTCCTCACCTCTCTTCAATCTCTTCAACTCTGTGATTTTCCAAAGCTGGCATCTATTCCAGTGGAGGGGTTGCCTATTTCACTGAGGGAACTTAAAATCATTAGGTGTCCTCTGCTAAAAGATAAATGCCAGCCTGGAAGTAAAGGACGATACCTGCCCAAAATATCCCACATCCCCCGCATAGAGATATGGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1408

Amino Acids

159.34

Weight (kDa)

6.97

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 439
AatII GACGTC 1 cut(s) 440
Acc36I ACCTGC 2 cut(s) 514, 4188
Acc65I GGTACC 1 cut(s) 3082
AccB1I GGYRCC 3 cut(s) 839, 2635, 3082
AccB7I CCANNNNNTGG 2 cut(s) 2399, 3164
AccBSI CCGCTC 1 cut(s) 143
AccI GTMKAC 2 cut(s) 42, 96
AccII CGCG 3 cut(s) 55, 885, 922
AccIII TCCGGA 1 cut(s) 740
AclWI GGATC 4 cut(s) 1413, 2082, 2095, 2571
AcuI CTGAAG 5 cut(s) 215, 1653, 2927, 3731, 4019
AcyI GRCGYC 2 cut(s) 93, 437
AfaI GTAC 6 cut(s) 74, 475, 1548, 2358, 2502, 3084
AfiI CCNNNNNNNGG 9 cut(s) 303, 500, 2006, 2399, 2558, 2833, 3164, 3913, 4091
AflIII ACRYGT 4 cut(s) 476, 920, 3368, 3726
AjiI CACGTC 1 cut(s) 455
AjnI CCWGG 2 cut(s) 2793, 4159
AleI CACNNNNGTG 1 cut(s) 2348
Alw21I GWGCWC 1 cut(s) 3354
Alw26I GTCTC 2 cut(s) 2501, 2753
AlwI GGATC 4 cut(s) 1413, 2082, 2095, 2571
AlwNI CAGNNNCTG 1 cut(s) 1888
Ama87I CYCGRG 2 cut(s) 2639, 3622
Aor13HI TCCGGA 1 cut(s) 740
AoxI GGCC 5 cut(s) 1126, 1569, 2163, 3556, 3666
ArsI GACNNNNNNTTYG 6 cut(s) 510, 542, 983, 1015, 1046, 1078
Asp700I GAANNNNTTC 1 cut(s) 1643
Asp718I GGTACC 1 cut(s) 3082
AspLEI GCGC 3 cut(s) 55, 555, 3421
AspS9I GGNCC 4 cut(s) 491, 1570, 2462, 3556
AsuHPI GGTGA 9 cut(s) 169, 2704, 2830, 3092, 3562, 3647, 3782, 3862, 4024
AsuII TTCGAA 2 cut(s) 938, 3077
AvaI CYCGRG 2 cut(s) 2639, 3622
AvaII GGWCC 2 cut(s) 491, 2462
BaeGI GKGCMC 2 cut(s) 842, 2640
BaeI ACNNNNGTAYC 2 cut(s) 3257, 3290
BamHI GGATCC 1 cut(s) 2087
BanI GGYRCC 3 cut(s) 839, 2635, 3082
BanII GRGCYC 1 cut(s) 3403
BbsI GAAGAC 5 cut(s) 246, 519, 3315, 3737, 3909
Bbv12I GWGCWC 1 cut(s) 3354
BbvCI CCTCAGC 1 cut(s) 2148
BccI CCATC 8 cut(s) 482, 881, 940, 1324, 2106, 2732, 3467, 3626
BceAI ACGGC 2 cut(s) 545, 3123
BcgI CGANNNNNNTGC 8 cut(s) 560, 594, 918, 952, 1991, 2025, 3057, 3091
BciT130I CCWGG 2 cut(s) 2795, 4161
BcoDI GTCTC 2 cut(s) 2501, 2753
BfaI CTAG 7 cut(s) 701, 962, 1113, 1943, 2006, 2204, 3050
BfmI CTRYAG 5 cut(s) 107, 753, 844, 869, 3455
BfoI RGCGCY 1 cut(s) 3422
BfuAI ACCTGC 2 cut(s) 514, 4188
BmcAI AGTACT 2 cut(s) 74, 2502
Bme1390I CCNGG 2 cut(s) 2795, 4161
Bme18I GGWCC 2 cut(s) 491, 2462
BmeT110I CYCGRG 2 cut(s) 2639, 3622
BmgBI CACGTC 1 cut(s) 455
BmgT120I GGNCC 4 cut(s) 491, 1570, 2462, 3556
BmiI GGNNCC 6 cut(s) 841, 978, 2089, 2637, 2825, 3084
BmrFI CCNGG 2 cut(s) 2795, 4161
BmrI ACTGGG 3 cut(s) 438, 465, 3397
BmuI ACTGGG 3 cut(s) 438, 465, 3397
BpiI GAAGAC 5 cut(s) 246, 519, 3315, 3737, 3909
BpmI CTGGAG 4 cut(s) 618, 2525, 3544, 3655
Bpu10I CCTNAGC 1 cut(s) 2148
Bpu14I TTCGAA 2 cut(s) 938, 3077
BpuEI CTTGAG 6 cut(s) 1052, 1954, 2107, 2534, 3124, 3368
Bsa29I ATCGAT 1 cut(s) 2142
BsaBI GATNNNNATC 1 cut(s) 765
BsaHI GRCGYC 2 cut(s) 93, 437
BsaJI CCNNGG 4 cut(s) 2178, 2793, 2794, 3414
BsaWI WCCGGW 4 cut(s) 740, 1213, 3550, 3721
BsaXI ACNNNNNCTCC 2 cut(s) 968, 998
Bsc4I CCNNNNNNNGG 9 cut(s) 303, 500, 2006, 2399, 2558, 2833, 3164, 3913, 4091
Bse118I RCCGGY 1 cut(s) 2165
Bse1I ACTGG 8 cut(s) 433, 444, 471, 2498, 2508, 2540, 3403, 4085
Bse3DI GCAATG 2 cut(s) 562, 1998
Bse8I GATNNNNATC 1 cut(s) 765
BseAI TCCGGA 1 cut(s) 740
BseBI CCWGG 2 cut(s) 2795, 4161
BseCI ATCGAT 1 cut(s) 2142
BseDI CCNNGG 4 cut(s) 2178, 2793, 2794, 3414
BseJI GATNNNNATC 1 cut(s) 765
BseLI CCNNNNNNNGG 9 cut(s) 303, 500, 2006, 2399, 2558, 2833, 3164, 3913, 4091
BseMI GCAATG 2 cut(s) 562, 1998
BseNI ACTGG 8 cut(s) 433, 444, 471, 2498, 2508, 2540, 3403, 4085
BseRI GAGGAG 4 cut(s) 176, 2903, 3020, 3320
BseSI GKGCMC 2 cut(s) 842, 2640
BseYI CCCAGC 1 cut(s) 2989
BsgI GTGCAG 1 cut(s) 606
Bsh1236I CGCG 3 cut(s) 55, 885, 922
BshFI GGCC 5 cut(s) 1128, 1571, 2165, 3558, 3668
BshNI GGYRCC 3 cut(s) 839, 2635, 3082
BshVI ATCGAT 1 cut(s) 2142
BsiHKAI GWGCWC 1 cut(s) 3354
BsiHKCI CYCGRG 2 cut(s) 2639, 3622
BsiSI CCGG 5 cut(s) 741, 1214, 2166, 3551, 3722
BslFI GGGAC 4 cut(s) 233, 445, 859, 2080
BslI CCNNNNNNNGG 9 cut(s) 303, 500, 2006, 2399, 2558, 2833, 3164, 3913, 4091
BsmAI GTCTC 2 cut(s) 2501, 2753
BsmFI GGGAC 4 cut(s) 233, 445, 859, 2080
BsmI GAATGC 2 cut(s) 23, 1022
BsnI GGCC 5 cut(s) 1128, 1571, 2165, 3558, 3668
BsoBI CYCGRG 2 cut(s) 2639, 3622
Bsp119I TTCGAA 2 cut(s) 938, 3077
Bsp1286I GDGCHC 4 cut(s) 842, 2640, 3354, 3403
Bsp13I TCCGGA 1 cut(s) 740
Bsp1407I TGTACA 1 cut(s) 1546
Bsp68I TCGCGA 1 cut(s) 885
BspANI GGCC 5 cut(s) 1128, 1571, 2165, 3558, 3668
BspDI ATCGAT 1 cut(s) 2142
BspEI TCCGGA 1 cut(s) 740
BspFNI CGCG 3 cut(s) 55, 885, 922
BspLI GGNNCC 6 cut(s) 841, 978, 2089, 2637, 2825, 3084
BspMAI CTGCAG 3 cut(s) 111, 873, 3459
BspMI ACCTGC 2 cut(s) 514, 4188
BspPI GGATC 4 cut(s) 1413, 2082, 2095, 2571
BspQI GCTCTTC 1 cut(s) 3117
BspT104I TTCGAA 2 cut(s) 938, 3077
BspT107I GGYRCC 3 cut(s) 839, 2635, 3082
BsrBI CCGCTC 1 cut(s) 143
BsrDI GCAATG 2 cut(s) 562, 1998
BsrFI RCCGGY 1 cut(s) 2165
BsrGI TGTACA 1 cut(s) 1546
BsrI ACTGG 8 cut(s) 433, 444, 471, 2498, 2508, 2540, 3403, 4085
BssAI RCCGGY 1 cut(s) 2165
BssECI CCNNGG 4 cut(s) 2178, 2793, 2794, 3414
BssNI GRCGYC 2 cut(s) 93, 437
BssT1I CCWWGG 2 cut(s) 2178, 3414
Bst2UI CCWGG 2 cut(s) 2795, 4161
Bst4CI ACNGT 5 cut(s) 747, 1491, 3743, 3770, 3935
Bst6I CTCTTC 5 cut(s) 2977, 3117, 3334, 4044, 4053
BstACI GRCGYC 2 cut(s) 93, 437
BstAPI GCANNNNNTGC 1 cut(s) 1741
BstAUI TGTACA 1 cut(s) 1546
BstBI TTCGAA 2 cut(s) 938, 3077
BstC8I GCNNGC 9 cut(s) 188, 955, 1020, 1573, 2167, 2243, 2489, 4074, 4157
BstFNI CGCG 3 cut(s) 55, 885, 922
BstH2I RGCGCY 1 cut(s) 3422
BstHHI GCGC 3 cut(s) 55, 555, 3421
BstMAI GTCTC 2 cut(s) 2501, 2753
BstNI CCWGG 2 cut(s) 2795, 4161
BstNSI RCATGY 5 cut(s) 480, 1022, 2357, 3372, 3730
BstSCI CCNGG 2 cut(s) 2793, 4159
BstSFI CTRYAG 5 cut(s) 107, 753, 844, 869, 3455
BstSLI GKGCMC 2 cut(s) 842, 2640
BstUI CGCG 3 cut(s) 55, 885, 922
BstV2I GAAGAC 5 cut(s) 246, 519, 3315, 3737, 3909
BstX2I RGATCY 2 cut(s) 1405, 2087
BstXI CCANNNNNNTGG 1 cut(s) 672
BstYI RGATCY 2 cut(s) 1405, 2087
Bsu15I ATCGAT 1 cut(s) 2142
BsuRI GGCC 5 cut(s) 1128, 1571, 2165, 3558, 3668
BsuTUI ATCGAT 1 cut(s) 2142
BtgZI GCGATG 1 cut(s) 900
BtrI CACGTC 1 cut(s) 455
BtsI GCAGTG 2 cut(s) 1099, 2454
BtsIMutI CAGTG 9 cut(s) 752, 1099, 1496, 1886, 1982, 2454, 2903, 4092, 4106
BtuMI TCGCGA 1 cut(s) 885
BveI ACCTGC 2 cut(s) 514, 4188
Cac8I GCNNGC 9 cut(s) 188, 955, 1020, 1573, 2167, 2243, 2489, 4074, 4157
CaiI CAGNNNCTG 1 cut(s) 1888
CfoI GCGC 3 cut(s) 55, 555, 3421
Cfr10I RCCGGY 1 cut(s) 2165
Cfr13I GGNCC 4 cut(s) 491, 1570, 2462, 3556
ClaI ATCGAT 1 cut(s) 2142
CseI GACGC 4 cut(s) 44, 82, 1569, 1943
Csp6I GTAC 6 cut(s) 73, 474, 1547, 2357, 2501, 3083
CviAII CATG 9 cut(s) 477, 1019, 1466, 1544, 1577, 2319, 2354, 3369, 3727
CviQI GTAC 6 cut(s) 73, 474, 1547, 2357, 2501, 3083
DraI TTTAAA 2 cut(s) 661, 3780
DrdI GACNNNNNNGTC 1 cut(s) 439
DseDI GACNNNNNNGTC 1 cut(s) 439
Eam1104I CTCTTC 5 cut(s) 2977, 3117, 3334, 4044, 4053
EarI CTCTTC 5 cut(s) 2977, 3117, 3334, 4044, 4053
EciI GGCGGA 2 cut(s) 173, 2895
Eco130I CCWWGG 2 cut(s) 2178, 3414
Eco24I GRGCYC 1 cut(s) 3403
Eco32I GATATC 1 cut(s) 390
Eco47I GGWCC 2 cut(s) 491, 2462
Eco57I CTGAAG 5 cut(s) 215, 1653, 2927, 3731, 4019
Eco88I CYCGRG 2 cut(s) 2639, 3622
EcoO109I RGGNCCY 1 cut(s) 3556
EcoRI GAATTC 1 cut(s) 2837
EcoRII CCWGG 2 cut(s) 2793, 4159
EcoRV GATATC 1 cut(s) 390
EcoT14I CCWWGG 2 cut(s) 2178, 3414
EcoT22I ATGCAT 2 cut(s) 1024, 1467
EcoT38I GRGCYC 1 cut(s) 3403
ErhI CCWWGG 2 cut(s) 2178, 3414
FaeI CATG 9 cut(s) 480, 1022, 1469, 1547, 1580, 2322, 2357, 3372, 3730
FalI AAGNNNNNCTT 4 cut(s) 393, 425, 2153, 2185
FaqI GGGAC 4 cut(s) 233, 445, 859, 2080
FatI CATG 9 cut(s) 476, 1018, 1465, 1543, 1576, 2318, 2353, 3368, 3726
FauI CCCGC 3 cut(s) 1580, 1737, 4211
FblI GTMKAC 2 cut(s) 42, 96
FriOI GRGCYC 1 cut(s) 3403
FspBI CTAG 7 cut(s) 701, 962, 1113, 1943, 2006, 2204, 3050
GlaI GCGC 3 cut(s) 54, 554, 3420
GsaI CCCAGC 1 cut(s) 2993
GsuI CTGGAG 4 cut(s) 618, 2525, 3544, 3655
HaeII RGCGCY 1 cut(s) 3422
HaeIII GGCC 5 cut(s) 1128, 1571, 2165, 3558, 3668
HapII CCGG 5 cut(s) 741, 1214, 2166, 3551, 3722
HgaI GACGC 4 cut(s) 44, 82, 1569, 1943
HhaI GCGC 3 cut(s) 55, 555, 3421
Hin1I GRCGYC 2 cut(s) 93, 437
Hin1II CATG 9 cut(s) 480, 1022, 1469, 1547, 1580, 2322, 2357, 3372, 3730
Hin6I GCGC 3 cut(s) 53, 553, 3419
HinP1I GCGC 3 cut(s) 53, 553, 3419
HincII GTYRAC 5 cut(s) 43, 97, 482, 1036, 2016
HindII GTYRAC 5 cut(s) 43, 97, 482, 1036, 2016
HindIII AAGCTT 3 cut(s) 197, 2270, 3038
HpaI GTTAAC 1 cut(s) 1036
HpaII CCGG 5 cut(s) 741, 1214, 2166, 3551, 3722
HphI GGTGA 9 cut(s) 169, 2704, 2830, 3092, 3562, 3647, 3782, 3862, 4024
Hpy166II GTNNAC 7 cut(s) 43, 97, 380, 482, 967, 1036, 2016
Hpy8I GTNNAC 7 cut(s) 43, 97, 380, 482, 967, 1036, 2016
Hpy99I CGWCG 4 cut(s) 60, 98, 439, 442
HpyCH4III ACNGT 5 cut(s) 747, 1491, 3743, 3770, 3935
HpyCH4IV ACGT 4 cut(s) 243, 437, 454, 993
HpySE526I ACGT 4 cut(s) 243, 437, 454, 993
Hsp92I GRCGYC 2 cut(s) 93, 437
Hsp92II CATG 9 cut(s) 480, 1022, 1469, 1547, 1580, 2322, 2357, 3372, 3730
HspAI GCGC 3 cut(s) 53, 553, 3419
Kpn2I TCCGGA 1 cut(s) 740
KpnI GGTACC 1 cut(s) 3086
KroI GCCGGC 1 cut(s) 2165
KroNI GCCGGC 1 cut(s) 2167
KspAI GTTAAC 1 cut(s) 1036
LguI GCTCTTC 1 cut(s) 3117
LmnI GCTCC 7 cut(s) 140, 728, 890, 950, 976, 2890, 3957
MaeI CTAG 7 cut(s) 701, 962, 1113, 1943, 2006, 2204, 3050
MaeII ACGT 4 cut(s) 243, 437, 454, 993
MbiI CCGCTC 1 cut(s) 143
MfeI CAATTG 5 cut(s) 135, 1313, 1791, 2621, 3017
MflI RGATCY 2 cut(s) 1405, 2087
MhlI GDGCHC 4 cut(s) 842, 2640, 3354, 3403
MluI ACGCGT 1 cut(s) 920
MlyI GAGTC 8 cut(s) 75, 609, 1070, 1111, 1195, 1708, 2503, 3616
MmeI TCCRAC 3 cut(s) 583, 2109, 2730
Mph1103I ATGCAT 2 cut(s) 1024, 1467
MroI TCCGGA 1 cut(s) 740
MroNI GCCGGC 1 cut(s) 2165
MroXI GAANNNNTTC 1 cut(s) 1643
MslI CAYNNNNRTG 1 cut(s) 2348
MspA1I CMGCKG 4 cut(s) 193, 3271, 3665, 3847
MspI CCGG 5 cut(s) 741, 1214, 2166, 3551, 3722
MspR9I CCNGG 2 cut(s) 2795, 4161
MunI CAATTG 5 cut(s) 135, 1313, 1791, 2621, 3017
Mva1269I GAATGC 2 cut(s) 23, 1022
MvaI CCWGG 2 cut(s) 2795, 4161
MvnI CGCG 3 cut(s) 55, 885, 922
NaeI GCCGGC 1 cut(s) 2167
NgoMIV GCCGGC 1 cut(s) 2165
NlaIII CATG 9 cut(s) 480, 1022, 1469, 1547, 1580, 2322, 2357, 3372, 3730
NlaIV GGNNCC 6 cut(s) 841, 978, 2089, 2637, 2825, 3084
NmuCI GTSAC 6 cut(s) 709, 733, 913, 1062, 1202, 1771
NruI TCGCGA 1 cut(s) 885
NsiI ATGCAT 2 cut(s) 1024, 1467
NspI RCATGY 5 cut(s) 480, 1022, 2357, 3372, 3730
NspV TTCGAA 2 cut(s) 938, 3077
OliI CACNNNNGTG 1 cut(s) 2348
PaeI GCATGC 1 cut(s) 1022
PasI CCCWGGG 1 cut(s) 2794
PciI ACATGT 3 cut(s) 476, 3368, 3726
PciSI GCTCTTC 1 cut(s) 3117
PctI GAATGC 2 cut(s) 23, 1022
PdiI GCCGGC 1 cut(s) 2167
PdmI GAANNNNTTC 1 cut(s) 1643
PflFI GACNNNGTC 3 cut(s) 436, 439, 747
PflMI CCANNNNNTGG 2 cut(s) 2399, 3164
PleI GAGTC 8 cut(s) 75, 608, 1069, 1111, 1194, 1707, 2503, 3615
PpsI GAGTC 8 cut(s) 75, 608, 1069, 1111, 1194, 1707, 2503, 3615
PscI ACATGT 3 cut(s) 476, 3368, 3726
Psp6I CCWGG 2 cut(s) 2793, 4159
PspFI CCCAGC 1 cut(s) 2989
PspGI CCWGG 2 cut(s) 2793, 4159
PspN4I GGNNCC 6 cut(s) 841, 978, 2089, 2637, 2825, 3084
PspPI GGNCC 4 cut(s) 491, 1570, 2462, 3556
PstI CTGCAG 3 cut(s) 111, 873, 3459
PstNI CAGNNNCTG 1 cut(s) 1888
PsuI RGATCY 2 cut(s) 1405, 2087
PsyI GACNNNGTC 3 cut(s) 436, 439, 747
RruI TCGCGA 1 cut(s) 885
RsaI GTAC 6 cut(s) 74, 475, 1548, 2358, 2502, 3084
RsaNI GTAC 6 cut(s) 73, 474, 1547, 2357, 2501, 3083
RseI CAYNNNNRTG 1 cut(s) 2348
SalI GTCGAC 2 cut(s) 41, 95
SapI GCTCTTC 1 cut(s) 3117
Sau96I GGNCC 4 cut(s) 491, 1570, 2462, 3556
ScaI AGTACT 2 cut(s) 74, 2502
SchI GAGTC 8 cut(s) 75, 609, 1070, 1111, 1195, 1708, 2503, 3616
ScrFI CCNGG 2 cut(s) 2795, 4161
SduI GDGCHC 4 cut(s) 842, 2640, 3354, 3403
SfcI CTRYAG 5 cut(s) 107, 753, 844, 869, 3455
SfuI TTCGAA 2 cut(s) 938, 3077
SinI GGWCC 2 cut(s) 491, 2462
SmiMI CAYNNNNRTG 1 cut(s) 2348
SmlI CTYRAG 6 cut(s) 1067, 1933, 2122, 2513, 3139, 3347
SmoI CTYRAG 6 cut(s) 1067, 1933, 2122, 2513, 3139, 3347
SphI GCATGC 1 cut(s) 1022
SspI AATATT 4 cut(s) 1167, 2809, 3027, 3760
SspMI CTAG 7 cut(s) 701, 962, 1113, 1943, 2006, 2204, 3050
StyD4I CCNGG 2 cut(s) 2793, 4159
StyI CCWWGG 2 cut(s) 2178, 3414
TaaI ACNGT 5 cut(s) 747, 1491, 3743, 3770, 3935
TaiI ACGT 4 cut(s) 246, 440, 457, 996
TaqII GACCGA 1 cut(s) 2450
TatI WGTACW 4 cut(s) 72, 473, 1546, 2500
TauI GCSGC 6 cut(s) 193, 1128, 1816, 2447, 3668, 3847
TscAI CASTG 9 cut(s) 752, 1099, 1496, 1893, 1989, 2454, 2910, 4092, 4113
TseFI GTSAC 6 cut(s) 709, 733, 913, 1062, 1202, 1771
Tsp45I GTSAC 6 cut(s) 709, 733, 913, 1062, 1202, 1771
TspGWI ACGGA 2 cut(s) 2173, 2228
TspRI CASTG 9 cut(s) 752, 1099, 1496, 1893, 1989, 2454, 2910, 4092, 4113
Tth111I GACNNNGTC 3 cut(s) 436, 439, 747
Van91I CCANNNNNTGG 2 cut(s) 2399, 3164
VpaK11BI GGWCC 2 cut(s) 491, 2462
XbaI TCTAGA 1 cut(s) 3049
XceI RCATGY 5 cut(s) 480, 1022, 2357, 3372, 3730
XcmI CCANNNNNNNNNTGG 1 cut(s) 589
XmiI GTMKAC 2 cut(s) 42, 96
XmnI GAANNNNTTC 1 cut(s) 1643
XspI CTAG 7 cut(s) 701, 962, 1113, 1943, 2006, 2204, 3050
ZraI GACGTC 1 cut(s) 438
ZrmI AGTACT 2 cut(s) 74, 2502
Zsp2I ATGCAT 2 cut(s) 1024, 1467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.