Rmu_sc0005255.1_g000025

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005255.1
OG0000070
Physical Location & Seq
Forward (+)
110808 .. 114860
4053 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005255.1_g000025.1.cds

Sequence Viewer

Length: 4053 bp
atggcagtgggagaggtctttcttgctgcgttccttcaggtgctattcgacaggttagcctctcgtgagttcctcaaatttgcacggcagaagggcattggcgggaagcttgaaaaatggagtgaaacactgtctgcaattcgagcagtgttgtatgacgccgaggagaagcaactcataagcatgtcagtgaaagagtggctggatgatctcaaagacttggcttatgatgtggaggacatcttggacaggttttccactgacatgttacgattcaaggccaaggagcaaaatctggctggcacaagcaaggtacgaggcctctttcttaaatttaaatgcaacttcaatatgaactctgaaatggaaaagatttctgaacgattgctagaaatatctcaacgaaaaaacatgctcggtttgaataatagcggagctggatctagtgcggcatcccaaaggcgaacaagctcatgcttgcttgatggacctgtggttggaaggtatgaagacaaaaggaagattattgctttgttgtcaagagatgaacctagtactgtcaatttccatgtagttgcaattgttggcatgcctggagttgggaagactacacttgctaaatcagtattcaaggacaacaatattgccctggaacagttcgatcttaaggtatgggtatcagtgtctgatgattttgaccttgttagagtaacaaaagccattcttgaatcggtcacgtctgtgtattgtgacttcaaggaattcaataaagttcaagataacttgagcaaggagttagcaagtaaaaagtttctaattgttttggatgatgtttggaatacctgtgaccatgatcaatggacaaccctacagtctccctttcatgtcggagcaccaggaagtaagataattgtaacgactcgtgatgaaaaagttgcaagaatgacgagacccattgagatccactgtttgaagtatatgtcagatgatgattgttgggaagtctttcagcagcatgcattcatgaatgtcaacagtgggagaccacaaggttttgaattttttcgagagagtattgttagaaaatgtggcggattgcccttggctgcaaggactctcggtggtcttttaggttgtgaagacattgatgagtggaaaaaaatattgaacaatagaatatggagtctatcaaataagagtcacattcaccaagtgttgaaattgagctatcactatcttccttcatatttgaaaaggtgctttgcatattgctcaatactcccaaacgactatgaatttggggagaagcaattgacacttctgtggatggcagagggtttacttcaacaacaagaagaacatgaaacattagaagacttgggtggtcactattttggacagctattatctaggtcattatttcaaaaatcaagcagaaacagttcattatatgttatgcatcaccttgttggtgatctggctcggtgggctgcgggagaaatttgtctcaggttggaggataaagatggtagatgttctcgaaagacacgtcattcgtcatacattcccggtcagtatgatggggttaacaaatttgaggcattctctgaagccagacgtttgcgtactttcctaccattgtcagaagacttttgtaaccatgtgacttgtaaggttactcttgatttactgccaagattggaatacttacgggtactctctttgaatggctatcaaataactgagttgccgaattcaattggtaacttgaagcatctacggtaccttgatgtttctcatacattaataatgagattgcctgaatcaacaagcaccctttgcaacttacagacattaatattagaaagttgttctagactgaagacactgcctacaaagatgcgatatttatcaaatctacgccatctcaacaatttaaatgtgccttcattggaaggaatgcccccacagctaagtcaactgagcaatctgcaaacgttgccgaatttcgtggtgggcaaaggtagtgactcaagggtaagagagatagggcccctgtctcttcttcgagggacattgtgcctctcaagattggaaaatgtcaatgatgttgaggatgcaaggaggagtgacttaatttgcaaggaagggattgatgcattgcagctggaatggaatgataaaggagaaaatgaatcgatcgtgcttgacatgttaaaacctcacaaaaagatgaaagaggtcatcatcaagggttatggaggtttgcagttttcagcatggattggagatcctttattctcttacgtggtgcatgtgaggttagagaattgtaacaattgtcgattcttgccaccacttggacaattgccttctctccaaatactttgtattagaaatatgtctggggtggaaaaagttggcctagagttttgtggagagggtagcttgtcttttccattattggagacccttgtgtttgaggatatgcaaaactggaatgagtggtttccttgccaaggatatcaaggactagaagttttcccttccttgaaaatactttcagtgaaaagatgtcccaaattaaagggtgagttacctcacaagcttgatttgttaacaaagcttgagattcatgaatgtaatgaactggtggtttcaattggcaactacagacagctctgtgaattaaacgtgcgcgattgcaaaggggtggtgtataaaagtgtgcaccactttgagttactcaagtctatttatctttcagatttttcagtgctgagatttcaaactggaggattcatgaccggattaagaaaggtggaacaattgatgatagccaatggtgatgaactttcatctgaatggcagaatgaaggtagattgctgcaacacctgatatcttttcgaagtttgttcattgaaagaaactcccaccttcttcaattgcattgcctgacttcacttcaaatgcttcacatatatagatgcccaagtctagtttccattccagagtcatgtttgccagcttctcttaaagatatagagattgaaaggtgcggttctctgacgtgctttgcaagatacaaaatacccccgagtctaagaacaatagagattaaacattgccagtgtttgaaatatttggtagaggaggagcaggtggagggttgctcctctgcttcatcttctcttgagtacttgagcatatctaactgtccatctcttatctccttatcatccagaggtggattaccaaaggcacttaaacacctttacataagggattgtgaacaactggagacaatatctgaaggcttctatgatgacacttgcctcgaagaacttcatattgggcgttgtgcaaatctgaaattcttaccggagggcctatgtcttcttgccaatcttcgtaggttaattgtttttgtctgtggaagtcttgtttccttcccaagaggagggttgccgacaaggccctccaacctgacattccttgttatctctgattgtcaaagatggaaagccttccccaaaggcatgatcaacctcaactctctccaggagctggtgattggaagctgtgaaggtttggcgtccattctagaggagggttttcccccgaacctaacatcacttatccttttgaatcccagcaattgtaagccactcacagactggggcttgcacttgcacagactcacctctcttacagagttatgcatacaaggtgttgatcaagacttggtttcctttccaccagaggaaatgctgcttcctacatctctcattaaacttaccattggattcttcccgaatctgagacgaatatccagccagggccttcaagtcctcacctctcttgaatctcttagaattgatgattgcccaaagcttgcatccattccagaggacctgccctcttcactcacacaactttacatctatgatgactgtcctctgcttagaaagagatataaaccagatataccacgatactgggccaaaataagtcacattccgtacatagagattggagactacaaaccctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1350

Amino Acids

153.37

Weight (kDa)

6.73

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3151
Acc36I ACCTGC 2 cut(s) 3151, 3924
Acc65I GGTACC 1 cut(s) 1782
AccB1I GGYRCC 1 cut(s) 1782
AccB7I CCANNNNNTGG 2 cut(s) 2360, 3568
AccII CGCG 1 cut(s) 2700
AciI CCGC 6 cut(s) 102, 432, 449, 1092, 1493, 3060
AclI AACGTT 1 cut(s) 1997
AclWI GGATC 3 cut(s) 448, 955, 2285
AcuI CTGAAG 4 cut(s) 20, 1629, 1901, 3333
AcyI GRCGYC 2 cut(s) 159, 3596
AdeI CACNNNGTG 1 cut(s) 1213
AfaI GTAC 7 cut(s) 315, 556, 1627, 1716, 1784, 3200, 4025
AfiI CCNNNNNNNGG 6 cut(s) 497, 599, 2360, 2519, 3461, 3568
AflII CTTAAG 1 cut(s) 665
AflIII ACRYGT 3 cut(s) 264, 1547, 2211
AjiI CACGTC 3 cut(s) 738, 1550, 3072
AjnI CCWGG 5 cut(s) 592, 648, 895, 3561, 3837
AjuI GAANNNNNNNTTGG 6 cut(s) 2986, 3018, 3336, 3368, 3476, 3508
AleI CACNNNNGTG 2 cut(s) 740, 1321
Alw21I GWGCWC 2 cut(s) 895, 2733
Alw26I GTCTC 9 cut(s) 879, 943, 1036, 1511, 2064, 2462, 3296, 3817, 4032
Alw44I GTGCAC 1 cut(s) 2729
AlwI GGATC 3 cut(s) 448, 955, 2285
AlwNI CAGNNNCTG 2 cut(s) 686, 3568
Ama87I CYCGRG 1 cut(s) 3097
AoxI GGCC 8 cut(s) 279, 319, 2051, 2422, 3388, 3476, 3841, 4002
ApaI GGGCCC 1 cut(s) 2055
ApaLI GTGCAC 1 cut(s) 2729
ApeKI GCWGC 7 cut(s) 26, 1012, 1106, 1490, 2164, 2887, 3772
ArsI GACNNNNNNTTYG 6 cut(s) 1280, 1312, 1534, 1537, 1566, 1569
AseI ATTAAT 2 cut(s) 1805, 1856
Asp700I GAANNNNTTC 5 cut(s) 1005, 1062, 1441, 3345, 3527
Asp718I GGTACC 1 cut(s) 1782
AspLEI GCGC 1 cut(s) 2700
AspS9I GGNCC 8 cut(s) 488, 2051, 2052, 3388, 3477, 3841, 3913, 4002
AsuC2I CCSGG 1 cut(s) 1569
AsuHPI GGTGA 8 cut(s) 1199, 1454, 1484, 2603, 2858, 3583, 3694, 3847
AsuII TTCGAA 1 cut(s) 2908
AvaI CYCGRG 1 cut(s) 3097
AvaII GGWCC 2 cut(s) 488, 3913
BaeGI GKGCMC 2 cut(s) 2055, 2733
BanI GGYRCC 1 cut(s) 1782
BanII GRGCYC 1 cut(s) 2055
BarI GAAGNNNNNNTAC 2 cut(s) 737, 769
BauI CACGAG 2 cut(s) 63, 921
BbsI GAAGAC 7 cut(s) 516, 611, 1146, 1380, 1653, 1889, 3389
Bbv12I GWGCWC 2 cut(s) 895, 2733
BbvI GCAGC 7 cut(s) 13, 1024, 1093, 1477, 2176, 2874, 3759
BccI CCATC 7 cut(s) 479, 1321, 1520, 1574, 1932, 3229, 3513
BceAI ACGGC 1 cut(s) 101
BciT130I CCWGG 5 cut(s) 594, 650, 897, 3563, 3839
BclI TGATCA 3 cut(s) 853, 3543, 3736
BcnI CCSGG 1 cut(s) 1569
BcoDI GTCTC 9 cut(s) 879, 943, 1036, 1511, 2064, 2462, 3296, 3817, 4032
BfaI CTAG 9 cut(s) 389, 444, 552, 1410, 1875, 2426, 2534, 2999, 3605
BfmI CTRYAG 2 cut(s) 869, 2671
BfrI CTTAAG 1 cut(s) 665
BfuAI ACCTGC 2 cut(s) 3151, 3924
BglI GCCNNNNNGGC 1 cut(s) 3475
BisI GCNGC 8 cut(s) 27, 450, 1013, 1107, 1491, 2165, 2888, 3773
BlsI GCNGC 8 cut(s) 28, 451, 1014, 1108, 1492, 2166, 2889, 3774
BmcAI AGTACT 2 cut(s) 556, 3200
Bme1390I CCNGG 6 cut(s) 594, 650, 897, 1569, 3563, 3839
Bme18I GGWCC 2 cut(s) 488, 3913
BmeT110I CYCGRG 1 cut(s) 3097
BmgBI CACGTC 3 cut(s) 738, 1550, 3072
BmgT120I GGNCC 8 cut(s) 488, 2051, 2052, 3388, 3477, 3841, 3913, 4002
BmiI GGNNCC 3 cut(s) 1784, 2053, 2054
BmrFI CCNGG 6 cut(s) 594, 650, 897, 1569, 3563, 3839
BmrI ACTGGG 2 cut(s) 3688, 4009
BmsI GCATC 8 cut(s) 461, 1468, 1783, 1890, 2107, 2146, 2978, 3908
BmuI ACTGGG 2 cut(s) 3688, 4009
BpiI GAAGAC 7 cut(s) 516, 611, 1146, 1380, 1653, 1889, 3389
BplI GAGNNNNNCTC 4 cut(s) 1589, 1621, 3158, 3190
BpmI CTGGAG 4 cut(s) 615, 2814, 3320, 3545
Bpu14I TTCGAA 1 cut(s) 2908
BpuEI CTTGAG 7 cut(s) 805, 2017, 2071, 2648, 2732, 3215, 3223
BpuMI CCSGG 1 cut(s) 1569
Bsa29I ATCGAT 1 cut(s) 2198
BsaAI YACGTR 1 cut(s) 2308
BsaBI GATNNNNATC 1 cut(s) 1909
BsaHI GRCGYC 2 cut(s) 159, 3596
BsaI GGTCTC 3 cut(s) 943, 1036, 2462
BsaJI CCNNGG 6 cut(s) 162, 282, 648, 1101, 2518, 3838
BsaWI WCCGGW 2 cut(s) 2807, 3382
Bsc4I CCNNNNNNNGG 6 cut(s) 497, 599, 2360, 2519, 3461, 3568
Bse1I ACTGG 7 cut(s) 2501, 2655, 2797, 3130, 3303, 3683, 4004
Bse3DI GCAATG 3 cut(s) 2159, 2950, 3124
Bse8I GATNNNNATC 1 cut(s) 1909
BseBI CCWGG 5 cut(s) 594, 650, 897, 3563, 3839
BseCI ATCGAT 1 cut(s) 2198
BseDI CCNNGG 6 cut(s) 162, 282, 648, 1101, 2518, 3838
BseGI GGATG 7 cut(s) 211, 452, 832, 1332, 2122, 3239, 3899
BseJI GATNNNNATC 1 cut(s) 1909
BseLI CCNNNNNNNGG 6 cut(s) 497, 599, 2360, 2519, 3461, 3568
BseMI GCAATG 3 cut(s) 2159, 2950, 3124
BseMII CTCAG 5 cut(s) 1522, 1734, 1973, 2771, 3812
BseNI ACTGG 7 cut(s) 2501, 2655, 2797, 3130, 3303, 3683, 4004
BseRI GAGGAG 7 cut(s) 179, 2140, 3166, 3167, 3170, 3474, 3623
BseSI GKGCMC 2 cut(s) 2055, 2733
BseXI GCAGC 7 cut(s) 13, 1024, 1093, 1477, 2176, 2874, 3759
BseYI CCCAGC 1 cut(s) 3653
Bsh1236I CGCG 1 cut(s) 2700
Bsh1285I CGRYCG 1 cut(s) 2202
BshFI GGCC 8 cut(s) 281, 321, 2053, 2424, 3390, 3478, 3843, 4004
BshNI GGYRCC 1 cut(s) 1782
BshVI ATCGAT 1 cut(s) 2198
BsiEI CGRYCG 1 cut(s) 2202
BsiHKAI GWGCWC 2 cut(s) 895, 2733
BsiHKCI CYCGRG 1 cut(s) 3097
BsiSI CCGG 3 cut(s) 1569, 2808, 3383
BslFI GGGAC 2 cut(s) 2086, 2562
BslI CCNNNNNNNGG 6 cut(s) 497, 599, 2360, 2519, 3461, 3568
BsmAI GTCTC 9 cut(s) 879, 943, 1036, 1511, 2064, 2462, 3296, 3817, 4032
BsmBI CGTCTC 1 cut(s) 3817
BsmFI GGGAC 2 cut(s) 2086, 2562
BsmI GAATGC 3 cut(s) 1019, 1601, 1965
BsnI GGCC 8 cut(s) 281, 321, 2053, 2424, 3390, 3478, 3843, 4004
Bso31I GGTCTC 3 cut(s) 943, 1036, 2462
BsoBI CYCGRG 1 cut(s) 3097
Bsp119I TTCGAA 1 cut(s) 2908
Bsp120I GGGCCC 1 cut(s) 2051
Bsp1286I GDGCHC 3 cut(s) 895, 2055, 2733
BspACI CCGC 6 cut(s) 102, 432, 449, 1092, 1493, 3060
BspANI GGCC 8 cut(s) 281, 321, 2053, 2424, 3390, 3478, 3843, 4004
BspCNI CTCAG 5 cut(s) 1521, 1735, 1974, 2772, 3813
BspDI ATCGAT 1 cut(s) 2198
BspFNI CGCG 1 cut(s) 2700
BspHI TCATGA 3 cut(s) 1023, 2635, 2802
BspLI GGNNCC 3 cut(s) 1784, 2053, 2054
BspMI ACCTGC 2 cut(s) 3151, 3924
BspPI GGATC 3 cut(s) 448, 955, 2285
BspT104I TTCGAA 1 cut(s) 2908
BspT107I GGYRCC 1 cut(s) 1782
BspTI CTTAAG 1 cut(s) 665
BspTNI GGTCTC 3 cut(s) 943, 1036, 2462
BsrDI GCAATG 3 cut(s) 2159, 2950, 3124
BsrI ACTGG 7 cut(s) 2501, 2655, 2797, 3130, 3303, 3683, 4004
BssECI CCNNGG 6 cut(s) 162, 282, 648, 1101, 2518, 3838
BssNI GRCGYC 2 cut(s) 159, 3596
BssSI CACGAG 2 cut(s) 63, 921
BssT1I CCWWGG 3 cut(s) 282, 1101, 2518
Bst2BI CACGAG 2 cut(s) 63, 921
Bst2UI CCWGG 5 cut(s) 594, 650, 897, 3563, 3839
Bst6I CTCTTC 2 cut(s) 2067, 3928
BstACI GRCGYC 2 cut(s) 159, 3596
BstAFI CTTAAG 1 cut(s) 665
BstAPI GCANNNNNTGC 2 cut(s) 1839, 1999
BstBAI YACGTR 1 cut(s) 2308
BstBI TTCGAA 1 cut(s) 2908
BstC8I GCNNGC 7 cut(s) 301, 479, 590, 1017, 3027, 3686, 3897
BstDEI CTNAG 9 cut(s) 1508, 1743, 1973, 1982, 2780, 3104, 3821, 3872, 3965
BstENI CCTNNNNNAGG 1 cut(s) 2517
BstF5I GGATG 7 cut(s) 211, 452, 832, 1332, 2122, 3239, 3899
BstFNI CGCG 1 cut(s) 2700
BstHHI GCGC 1 cut(s) 2700
BstMAI GTCTC 9 cut(s) 879, 943, 1036, 1511, 2064, 2462, 3296, 3817, 4032
BstMCI CGRYCG 1 cut(s) 2202
BstMWI GCNNNNNNNGC 6 cut(s) 143, 1487, 1839, 1969, 1999, 3475
BstNI CCWGG 5 cut(s) 594, 650, 897, 3563, 3839
BstNSI RCATGY 7 cut(s) 187, 268, 415, 592, 1019, 2215, 2318
BstSCI CCNGG 6 cut(s) 592, 648, 895, 1567, 3561, 3837
BstSFI CTRYAG 2 cut(s) 869, 2671
BstSLI GKGCMC 2 cut(s) 2055, 2733
BstUI CGCG 1 cut(s) 2700
BstV1I GCAGC 7 cut(s) 13, 1024, 1093, 1477, 2176, 2874, 3759
BstV2I GAAGAC 7 cut(s) 516, 611, 1146, 1380, 1653, 1889, 3389
BstX2I RGATCY 3 cut(s) 440, 960, 2290
BstXI CCANNNNNNTGG 1 cut(s) 3999
BstYI RGATCY 3 cut(s) 440, 960, 2290
Bsu15I ATCGAT 1 cut(s) 2198
BsuRI GGCC 8 cut(s) 281, 321, 2053, 2424, 3390, 3478, 3843, 4004
BsuTUI ATCGAT 1 cut(s) 2198
BtrI CACGTC 3 cut(s) 738, 1550, 3072
BtsCI GGATG 7 cut(s) 211, 452, 832, 1332, 2122, 3239, 3899
BtsI GCAGTG 3 cut(s) 12, 153, 1886
BveI ACCTGC 2 cut(s) 3151, 3924
Cac8I GCNNGC 7 cut(s) 301, 479, 590, 1017, 3027, 3686, 3897
CaiI CAGNNNCTG 2 cut(s) 686, 3568
CciI TCATGA 3 cut(s) 1023, 2635, 2802
CfoI GCGC 1 cut(s) 2700
Cfr13I GGNCC 8 cut(s) 488, 2051, 2052, 3388, 3477, 3841, 3913, 4002
ClaI ATCGAT 1 cut(s) 2198
CseI GACGC 2 cut(s) 167, 3585
Csp6I GTAC 7 cut(s) 314, 555, 1626, 1715, 1783, 3199, 4024
CviQI GTAC 7 cut(s) 314, 555, 1626, 1715, 1783, 3199, 4024
DdeI CTNAG 9 cut(s) 1508, 1743, 1973, 1982, 2780, 3104, 3821, 3872, 3965
DraI TTTAAA 2 cut(s) 337, 1938
DraIII CACNNNGTG 1 cut(s) 1213
Eam1104I CTCTTC 2 cut(s) 2067, 3928
EarI CTCTTC 2 cut(s) 2067, 3928
EciI GGCGGA 1 cut(s) 1107
Eco130I CCWWGG 3 cut(s) 282, 1101, 2518
Eco147I AGGCCT 1 cut(s) 321
Eco24I GRGCYC 1 cut(s) 2055
Eco31I GGTCTC 3 cut(s) 943, 1036, 2462
Eco32I GATATC 2 cut(s) 2525, 2901
Eco47I GGWCC 2 cut(s) 488, 3913
Eco57I CTGAAG 4 cut(s) 20, 1629, 1901, 3333
Eco88I CYCGRG 1 cut(s) 3097
EcoNI CCTNNNNNAGG 1 cut(s) 2517
EcoO109I RGGNCCY 6 cut(s) 2051, 2052, 3388, 3477, 3841, 3913
EcoRI GAATTC 2 cut(s) 761, 1753
EcoRII CCWGG 5 cut(s) 592, 648, 895, 3561, 3837
EcoRV GATATC 2 cut(s) 2525, 2901
EcoT14I CCWWGG 3 cut(s) 282, 1101, 2518
EcoT22I ATGCAT 4 cut(s) 1021, 1461, 2161, 3725
EcoT38I GRGCYC 1 cut(s) 2055
ErhI CCWWGG 3 cut(s) 282, 1101, 2518
Esp3I CGTCTC 1 cut(s) 3817
FalI AAGNNNNNCTT 2 cut(s) 708, 740
FaqI GGGAC 2 cut(s) 2086, 2562
FauI CCCGC 2 cut(s) 95, 1486
FbaI TGATCA 3 cut(s) 853, 3543, 3736
Fnu4HI GCNGC 8 cut(s) 27, 450, 1013, 1107, 1491, 2165, 2888, 3773
FokI GGATG 7 cut(s) 218, 439, 839, 1339, 2129, 3226, 3886
FriOI GRGCYC 1 cut(s) 2055
Fsp4HI GCNGC 8 cut(s) 27, 450, 1013, 1107, 1491, 2165, 2888, 3773
FspBI CTAG 9 cut(s) 389, 444, 552, 1410, 1875, 2426, 2534, 2999, 3605
GlaI GCGC 1 cut(s) 2699
GluI GCNGC 8 cut(s) 27, 450, 1013, 1107, 1491, 2165, 2888, 3773
GsaI CCCAGC 1 cut(s) 3657
GsuI CTGGAG 4 cut(s) 615, 2814, 3320, 3545
HaeIII GGCC 8 cut(s) 281, 321, 2053, 2424, 3390, 3478, 3843, 4004
HapII CCGG 3 cut(s) 1569, 2808, 3383
HgaI GACGC 2 cut(s) 167, 3585
HhaI GCGC 1 cut(s) 2700
Hin1I GRCGYC 2 cut(s) 159, 3596
Hin6I GCGC 1 cut(s) 2698
HinP1I GCGC 1 cut(s) 2698
HincII GTYRAC 4 cut(s) 1033, 1588, 1979, 2619
HindII GTYRAC 4 cut(s) 1033, 1588, 1979, 2619
HindIII AAGCTT 4 cut(s) 107, 2606, 2624, 3893
HpaI GTTAAC 2 cut(s) 1588, 2619
HpaII CCGG 3 cut(s) 1569, 2808, 3383
HphI GGTGA 8 cut(s) 1199, 1454, 1484, 2603, 2858, 3583, 3694, 3847
Hpy166II GTNNAC 7 cut(s) 1033, 1340, 1588, 1979, 2619, 2731, 3293
Hpy8I GTNNAC 7 cut(s) 1033, 1340, 1588, 1979, 2619, 2731, 3293
HpyCH4IV ACGT 7 cut(s) 737, 1549, 1618, 1997, 2307, 2694, 3071
HpyF10VI GCNNNNNNNGC 6 cut(s) 143, 1487, 1839, 1969, 1999, 3475
HpyF3I CTNAG 9 cut(s) 1508, 1743, 1973, 1982, 2780, 3104, 3821, 3872, 3965
HpySE526I ACGT 7 cut(s) 737, 1549, 1618, 1997, 2307, 2694, 3071
Hsp92I GRCGYC 2 cut(s) 159, 3596
HspAI GCGC 1 cut(s) 2698
KpnI GGTACC 1 cut(s) 1786
Ksp22I TGATCA 3 cut(s) 853, 3543, 3736
KspAI GTTAAC 2 cut(s) 1588, 2619
LmnI GCTCC 6 cut(s) 286, 434, 890, 3157, 3179, 3565
Lsp1109I GCAGC 7 cut(s) 13, 1024, 1093, 1477, 2176, 2874, 3759
LweI GCATC 8 cut(s) 461, 1468, 1783, 1890, 2107, 2146, 2978, 3908
MaeI CTAG 9 cut(s) 389, 444, 552, 1410, 1875, 2426, 2534, 2999, 3605
MaeII ACGT 7 cut(s) 737, 1549, 1618, 1997, 2307, 2694, 3071
MfeI CAATTG 9 cut(s) 579, 1310, 1758, 2338, 2366, 2661, 2828, 2945, 3658
MflI RGATCY 3 cut(s) 440, 960, 2290
MhlI GDGCHC 3 cut(s) 895, 2055, 2733
MlyI GAGTC 8 cut(s) 913, 1108, 1192, 1207, 2024, 3023, 3109, 3693
MmeI TCCRAC 4 cut(s) 478, 868, 1494, 3507
Mph1103I ATGCAT 4 cut(s) 1021, 1461, 2161, 3725
MroXI GAANNNNTTC 5 cut(s) 1005, 1062, 1441, 3345, 3527
MslI CAYNNNNRTG 6 cut(s) 182, 188, 263, 740, 1321, 2863
MspA1I CMGCKG 1 cut(s) 2167
MspCI CTTAAG 1 cut(s) 665
MspI CCGG 3 cut(s) 1569, 2808, 3383
MspR9I CCNGG 6 cut(s) 594, 650, 897, 1569, 3563, 3839
MunI CAATTG 9 cut(s) 579, 1310, 1758, 2338, 2366, 2661, 2828, 2945, 3658
Mva1269I GAATGC 3 cut(s) 1019, 1601, 1965
MvaI CCWGG 5 cut(s) 594, 650, 897, 3563, 3839
MvnI CGCG 1 cut(s) 2700
MwoI GCNNNNNNNGC 6 cut(s) 143, 1487, 1839, 1969, 1999, 3475
NciI CCSGG 1 cut(s) 1569
NlaIV GGNNCC 3 cut(s) 1784, 2053, 2054
NmeAIII GCCGAG 1 cut(s) 187
NmuCI GTSAC 9 cut(s) 733, 749, 845, 1199, 1385, 1663, 2027, 2129, 4013
NsiI ATGCAT 4 cut(s) 1021, 1461, 2161, 3725
NspI RCATGY 7 cut(s) 187, 268, 415, 592, 1019, 2215, 2318
NspV TTCGAA 1 cut(s) 2908
OliI CACNNNNGTG 2 cut(s) 740, 1321
PaeI GCATGC 2 cut(s) 592, 1019
PagI TCATGA 3 cut(s) 1023, 2635, 2802
PaqCI CACCTGC 1 cut(s) 3151
PceI AGGCCT 1 cut(s) 321
PciI ACATGT 2 cut(s) 264, 2211
PcsI WCGNNNNNNNCGW 1 cut(s) 1546
PctI GAATGC 3 cut(s) 1019, 1601, 1965
PdmI GAANNNNTTC 5 cut(s) 1005, 1062, 1441, 3345, 3527
PflMI CCANNNNNTGG 2 cut(s) 2360, 3568
PfoI TCCNGGA 1 cut(s) 3561
PkrI GCNGC 8 cut(s) 28, 451, 1014, 1108, 1492, 2166, 2889, 3774
Ple19I CGATCG 1 cut(s) 2202
PleI GAGTC 8 cut(s) 913, 1108, 1191, 1206, 2024, 3022, 3108, 3693
PpsI GAGTC 8 cut(s) 913, 1108, 1191, 1206, 2024, 3022, 3108, 3693
Ppu21I YACGTR 1 cut(s) 2308
PpuMI RGGWCCY 1 cut(s) 3913
PscI ACATGT 2 cut(s) 264, 2211
PshBI ATTAAT 2 cut(s) 1805, 1856
Psp1406I AACGTT 1 cut(s) 1997
Psp5II RGGWCCY 1 cut(s) 3913
Psp6I CCWGG 5 cut(s) 592, 648, 895, 3561, 3837
PspFI CCCAGC 1 cut(s) 3653
PspGI CCWGG 5 cut(s) 592, 648, 895, 3561, 3837
PspN4I GGNNCC 3 cut(s) 1784, 2053, 2054
PspOMI GGGCCC 1 cut(s) 2051
PspPI GGNCC 8 cut(s) 488, 2051, 2052, 3388, 3477, 3841, 3913, 4002
PspPPI RGGWCCY 1 cut(s) 3913
PstNI CAGNNNCTG 2 cut(s) 686, 3568
PsuI RGATCY 3 cut(s) 440, 960, 2290
PvuI CGATCG 1 cut(s) 2202
PvuII CAGCTG 1 cut(s) 2167
RsaI GTAC 7 cut(s) 315, 556, 1627, 1716, 1784, 3200, 4025
RsaNI GTAC 7 cut(s) 314, 555, 1626, 1715, 1783, 3199, 4024
RseI CAYNNNNRTG 6 cut(s) 182, 188, 263, 740, 1321, 2863
SatI GCNGC 8 cut(s) 27, 450, 1013, 1107, 1491, 2165, 2888, 3773
Sau96I GGNCC 8 cut(s) 488, 2051, 2052, 3388, 3477, 3841, 3913, 4002
ScaI AGTACT 2 cut(s) 556, 3200
SchI GAGTC 8 cut(s) 913, 1108, 1192, 1207, 2024, 3023, 3109, 3693
ScrFI CCNGG 6 cut(s) 594, 650, 897, 1569, 3563, 3839
SduI GDGCHC 3 cut(s) 895, 2055, 2733
SfaNI GCATC 8 cut(s) 461, 1468, 1783, 1890, 2107, 2146, 2978, 3908
SfcI CTRYAG 2 cut(s) 869, 2671
SfuI TTCGAA 1 cut(s) 2908
SinI GGWCC 2 cut(s) 488, 3913
SmiI ATTTAAAT 2 cut(s) 337, 1938
SmiMI CAYNNNNRTG 6 cut(s) 182, 188, 263, 740, 1321, 2863
SmlI CTYRAG 8 cut(s) 665, 784, 2032, 2086, 2627, 2747, 3194, 3202
SmoI CTYRAG 8 cut(s) 665, 784, 2032, 2086, 2627, 2747, 3194, 3202
SphI GCATGC 2 cut(s) 592, 1019
SseBI AGGCCT 1 cut(s) 321
SsiI CCGC 6 cut(s) 102, 432, 449, 1092, 1493, 3060
SspI AATATT 4 cut(s) 643, 1164, 1860, 3143
SspMI CTAG 9 cut(s) 389, 444, 552, 1410, 1875, 2426, 2534, 2999, 3605
StuI AGGCCT 1 cut(s) 321
StyD4I CCNGG 6 cut(s) 592, 648, 895, 1567, 3561, 3837
StyI CCWWGG 3 cut(s) 282, 1101, 2518
SwaI ATTTAAAT 2 cut(s) 337, 1938
TaiI ACGT 7 cut(s) 740, 1552, 1621, 2000, 2310, 2697, 3074
TaqII GACCGA 1 cut(s) 721
TatI WGTACW 2 cut(s) 554, 3198
TauI GCSGC 1 cut(s) 452
TseFI GTSAC 9 cut(s) 733, 749, 845, 1199, 1385, 1663, 2027, 2129, 4013
TseI GCWGC 7 cut(s) 26, 1012, 1106, 1490, 2164, 2887, 3772
Tsp45I GTSAC 9 cut(s) 733, 749, 845, 1199, 1385, 1663, 2027, 2129, 4013
TspGWI ACGGA 1 cut(s) 4011
Van91I CCANNNNNTGG 2 cut(s) 2360, 3568
Vha464I CTTAAG 1 cut(s) 665
VneI GTGCAC 1 cut(s) 2729
VpaK11BI GGWCC 2 cut(s) 488, 3913
VspI ATTAAT 2 cut(s) 1805, 1856
XagI CCTNNNNNAGG 1 cut(s) 2517
XbaI TCTAGA 2 cut(s) 1874, 3604
XceI RCATGY 7 cut(s) 187, 268, 415, 592, 1019, 2215, 2318
XcmI CCANNNNNNNNNTGG 1 cut(s) 3675
XmnI GAANNNNTTC 5 cut(s) 1005, 1062, 1441, 3345, 3527
XspI CTAG 9 cut(s) 389, 444, 552, 1410, 1875, 2426, 2534, 2999, 3605
ZrmI AGTACT 2 cut(s) 556, 3200
Zsp2I ATGCAT 4 cut(s) 1021, 1461, 2161, 3725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.