Rmu_sc0000724.1_g000006

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000724.1
OG0000070
Physical Location & Seq
Forward (+)
32538 .. 39663
7126 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000724.1_g000006.1.cds

Sequence Viewer

Length: 4827 bp
atggctctcactgatgagttgaagactctcaacaacgctttctcagggcttggagttgatgagaagtcattgatatcaatattgggaaactcgcatcctgaggagaggaaatgtttcaggaaaggaactccccatcttttcaaagaggatgaacgcctctttgagagatggaacgatgaccgtgtcagacttctcaagcatgaattcatgcgctttaagaatgctgtggtgttatgggctatgcatccatgggaaagagatgctcgcttggtgaaggaggctttgaagaaagggccagaagtctatggtgtcattgtagagattgcatgcactagatcctcagaagagctattaggagctagaaaggcctaccattccctctttgaccactccattgaagaagatgttgcctaccacattgatggccctgaaagcaagctcttggttgcacttgttagtgcctataggtatgaaggagcaaaggttaaagatgacaccgcaaaatctgaggcacaaacactctctcatgccattaagaatgcagataagacaaaccccatcgaagatgatgaggtgattaggatactatcaacaaggagcaaggcccatctccaggaagtatataaacactacaaggagatttctggccagaacatcgatgaggatcttgatgctgctttgaggttaaaagagacggtgcaatgcctatgtactcctcagacatatttcagcaaggtgctggaattggcattgaggaatgatgtggataagaacaccaaaaagggactgactagagtaattgtgacccgagctgacacagatatgaaagagatcaaagaggattacgagaaccaatatggagtttctctatccaataaaattgaagagacagctaatgggaactataaggatttcttgcttaccttggacgcggaggacaagaaactgacgagcgaggctgtgaagctatggctggatgaactcaaacacttggcttttgacatggatgacatcttggacaccttttccaccgagctgttgctgcgcaacgtgatgaatcagcaacatgcgtggcttacaagtaaggtacggggcctactttctagagctaagttcaatttcaatatgaattccgagatcgaggagattagtgataggttggtagagatatctgaaagaaaggacttgcttgggttgaattatgttgtgaataaatgtagtcaggtgtggcaaaggccgccgagttcatgtgtgttagatgggcctgtggttggtagggatgatgataaaaggaagattgttgaggtgttattgagggatgatcagcctagtagtgtcaatttccatgtggttgccattgtgggtatgccaggacttggaaagactacaattgcaggtcatgtcttcaatgatgatgcaatggaacggtttagtcccaaagtatgggtatctgtgtctgataacttcaatcttgtgagagtgacaaaggcaattctggaatcagttacatctgcacgctgtgatttggaggagttcagtcaaattcaggagagtctgagcaaggcattggcaagtaaaaagtttttggttgttttagatgatgtctggaacacatgtgactatgatctatgggcaaagctgcagtctgcctttcatggcggagcattgggaagtaaggtaattgtgacaactcgtgacacacaagttgctaagatgatgagatccattgaagttcataacttggggtgcatgccagatgatgattgctgggaagtctttgggcaacatgcattcttgaatgtcgttgagaatgggagactgcaaagctttgagttgtttcgggagaaagttgtagcaaaatgtgatgggttgcccttggctgcaagagtccttggtggtcttttaggctgtaaagagatagacgaatgggaagaaatattgaacagtaaattatggagtctatgtgataacagtggcattctcccagtattaaaattgagctatcactatctctcttcaaatttgaaaaggtgctttgcctattgctcaatacttccaaatgactatgagtttggggagaggcagttgatccttctatggatggcagagggtctcattcagcagtcacatgaatgtaaacaactggaagatttagggagtaactattttcgggagctattgtccaggtcattgtttcaaaggtccagtaaaaaccattcacaatttgttatgcatgaccttgttggtgatttgtcacggtgggctgcaggagacatgtgtttcagattggaggataagatggatgttagatgttcttcaaaggttcgccattcctcttacattcctggtcaatttgatggtgttcaaaaattcaaagaattttttgaagtcaggcatttgcggactttcttaccactttcactttcaggtcgatcaaattatctaactcgtaatgttacttttgatctattaccaaagttgaagtacttgagggcactctcttttaatggctatcaaataactgagttacccaattctattggtaagctgaagcatctgcggtaccttgatctttctcacacacagataacaactttgccagagtcaaccagcaatctgtacaacttacagacattgatattagaaaattgtgcttgtttgaaggcactgcctgcagacatgaggaatctaactgatctgcggcaccttaacaattcagatgtaccttcattggaagaaatgcctgtgaaactaggtagattgactaatcttcaaacactgagtacttttgtgctgggcaaaggtagcaagtcaggaataagtgagataggtcctttattgtgtctccaagggacattgtgcctctcaagattggagaatgtgactgatgttgatgatgcaaggagggctgacttgactagcaaggaagggattgattcattgcaactagaatggagagaatcaggagaaagggaatcagatgtttttgaaacgttaaaacctcataggaagctcaaagtgctcactgtaaaaggttatggaggtcttacattttcaacatggactggacacccttcattctcttatatgacgcatgtaaagttggaacattgtaaacattgtcgattcttgcctccacttggacagttgtcttctctgaaagaactttgtataaaaggaatgtctacagtggaaagcgtgggtcttgagttttatggagagagtagcctgccttttcctgtattggagaccctggagttcaaggatatgcagaaatggagggagtggcttcctcgggtgcaagatcaaggaattgaagttttcccttgcttgaaaatggtttgtatctctggatgccccatactggaggggagattacctgaaaaccttgatctattatcgaagcttgccattgatggatgcggccaattggtggtttcaattgccagttataaacgtcttcgtgatttgaaaattcatgcttgcaaaatgctggtacatacaagtggggttcaatttgagttactagaggcgatggagctttcatgtatttcagagtttagactccacatggaaggattcaagagagacttaacaaagcttaaaaatctgagaattactagttgtgaggagctgacatctttatggaaaaatgaggatagatttctgcaacgtccgatttatgaaggaaacagctctcactcttactttgttcaagagctgaagtcacttcaatgtcttcacataaatgattgctcaaatctagtttctttttcaaaggttagcctgccaccttctcttaaagaaataaagattgagcggtgcagttctcttataagataccagatacccccaaacgtaagaagtctatacatcgattcctgcataaacttggaatcattagtgggggaggacaaggaaggtgaggatccttcatcttccttttctccttgtttggtacaagaggaggcatcctgcctcgagtacttgagcatagtgaattgtatgagatttacaaccttatcatccacaggccagctagccaggggacttaaacatattatcataaatcattgtcaccagctagaatcaataactgatagcttccacgttaacaccttccttgaagaaattgtgattaggagttgtggaaatcttaaatcgttaccagagggcttatgtgacctcaccaatcttcaagtctttaagatttgggactgtgaaagtcttgtttctttcccaagaggagggtttccaacaagaacctccagcttgaaagagatcgaggttatgaattgtgataacttagaggcccttcctaaagacatgcagaacctccactgtcttcagagtttgaggatttcatactgtgacggtttcgctccatttctagacaagggtttgccacccaacctaacattccttgagattcgaaatctcaacagttgtaggccactcctggaagctcaaggattgcacagactcacttctcttagagcgttggatatcaggggaaatgattcacaactagtgtcatttccacctgacaaggagatggtgctccccaaatctctcattctacttcatattgcagggttcccgaatctgaggcacctatccagcaagggctttcagttcctcccctctcttgaatctcttcaagtcttcagctgtccaaagctagcatccattcctgaggagggactccctctttcacttacacaacttcatatcttaatttggttgtccactgctagtaaagaactgcagacctggaaaaggacaatactggcactccatagctcgcatccctttcatatggattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1608

Amino Acids

182.68

Weight (kDa)

6.07

Isoelectric Point (pI)

46.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3453, 3836
Acc16I TGCGCA 1 cut(s) 1046
Acc36I ACCTGC 1 cut(s) 1385
Acc65I GGTACC 1 cut(s) 2562
AccB1I GGYRCC 3 cut(s) 2562, 2703, 4611
AccB7I CCANNNNNTGG 3 cut(s) 1376, 1443, 3433
AccBSI CCGCTC 1 cut(s) 3820
AccI GTMKAC 1 cut(s) 3185
AccII CGCG 1 cut(s) 932
AciI CCGC 9 cut(s) 498, 932, 1238, 1659, 2401, 2560, 2701, 3423, 3820
AclI AACGTT 1 cut(s) 2993
AclWI GGATC 6 cut(s) 330, 672, 1716, 2083, 3923, 3936
AcoI YGGCCR 2 cut(s) 646, 3424
AcsI RAATTY 7 cut(s) 203, 1129, 1542, 2020, 2369, 2378, 3474
AcuI CTGAAG 4 cut(s) 2570, 3743, 4331, 4651
AdeI CACNNNGTG 1 cut(s) 1520
AflIII ACRYGT 2 cut(s) 1613, 2274
AhlI ACTAGT 2 cut(s) 3620, 4528
AjnI CCWGG 8 cut(s) 612, 1369, 2183, 2344, 3252, 4043, 4458, 4772
AjuI GAANNNNNNNTTGG 2 cut(s) 4403, 4435
Alw21I GWGCWC 2 cut(s) 3024, 4563
Alw26I GTCTC 8 cut(s) 686, 881, 1810, 2117, 2265, 2849, 3242, 3582
AlwI GGATC 6 cut(s) 330, 672, 1716, 2083, 3923, 3936
Ama87I CYCGRG 3 cut(s) 807, 3294, 3980
ApeKI GCWGC 5 cut(s) 674, 1042, 1639, 1880, 2264
ApoI RAATTY 7 cut(s) 203, 1129, 1542, 2020, 2369, 2378, 3474
ArsI GACNNNNNNTTYG 4 cut(s) 1628, 1660, 2054, 2086
Asp700I GAANNNNTTC 3 cut(s) 113, 4519, 4656
Asp718I GGTACC 1 cut(s) 2562
AspLEI GCGC 2 cut(s) 213, 1047
AspS9I GGNCC 8 cut(s) 293, 425, 604, 1093, 1262, 2202, 2831, 4312
AsuHPI GGTGA 6 cut(s) 283, 586, 2258, 3935, 4070, 4180
AsuII TTCGAA 1 cut(s) 4432
AsuNHI GCTAGC 2 cut(s) 4039, 4681
AvaI CYCGRG 3 cut(s) 807, 3294, 3980
AvaII GGWCC 2 cut(s) 2202, 2831
AxyI CCTNAGG 2 cut(s) 99, 4695
BaeGI GKGCMC 1 cut(s) 2497
BalI TGGCCA 1 cut(s) 648
BamHI GGATCC 1 cut(s) 3928
BanI GGYRCC 3 cut(s) 2562, 2703, 4611
BauI CACGAG 1 cut(s) 1692
BbsI GAAGAC 7 cut(s) 29, 1396, 3144, 3452, 3731, 4337, 4657
Bbv12I GWGCWC 2 cut(s) 3024, 4563
BbvI GCAGC 5 cut(s) 661, 1029, 1626, 1867, 2251
BciT130I CCWGG 8 cut(s) 614, 1371, 2185, 2346, 3254, 4045, 4460, 4774
BciVI GTATCC 1 cut(s) 576
BclI TGATCA 1 cut(s) 1321
BcoDI GTCTC 8 cut(s) 686, 881, 1810, 2117, 2265, 2849, 3242, 3582
BcuI ACTAGT 2 cut(s) 3620, 4528
BfmI CTRYAG 6 cut(s) 463, 1640, 2265, 2673, 3186, 4766
BfuAI ACCTGC 1 cut(s) 1385
BfuI GTATCC 1 cut(s) 576
BisI GCNGC 8 cut(s) 675, 1043, 1238, 1640, 1881, 2265, 2702, 3424
BlsI GCNGC 8 cut(s) 676, 1044, 1239, 1641, 1882, 2266, 2703, 3425
BmcAI AGTACT 3 cut(s) 2486, 2785, 3986
Bme1390I CCNGG 8 cut(s) 614, 1371, 2185, 2346, 3254, 4045, 4460, 4774
Bme18I GGWCC 2 cut(s) 2202, 2831
BmeT110I CYCGRG 3 cut(s) 807, 3294, 3980
BmgT120I GGNCC 8 cut(s) 293, 425, 604, 1093, 1262, 2202, 2831, 4312
BmiI GGNNCC 6 cut(s) 1094, 2564, 2705, 3930, 4598, 4613
BmrFI CCNGG 8 cut(s) 614, 1371, 2185, 2346, 3254, 4045, 4460, 4774
BmrI ACTGGG 1 cut(s) 1979
BmtI GCTAGC 2 cut(s) 4043, 4685
BmuI ACTGGG 1 cut(s) 1979
BoxI GACNNNNGTC 1 cut(s) 3148
BpiI GAAGAC 7 cut(s) 29, 1396, 3144, 3452, 3731, 4337, 4657
BpmI CTGGAG 4 cut(s) 596, 3275, 3386, 4252
Bpu14I TTCGAA 1 cut(s) 4432
BpuEI CTTGAG 7 cut(s) 179, 2509, 2851, 3227, 4009, 4445, 4452
Bsa29I ATCGAT 2 cut(s) 657, 3876
BsaBI GATNNNNATC 1 cut(s) 663
BsaI GGTCTC 2 cut(s) 2117, 3242
BsaJI CCNNGG 8 cut(s) 248, 924, 1875, 1891, 2848, 3252, 3293, 4044
Bse1I ACTGG 7 cut(s) 1985, 2148, 2205, 3070, 3369, 3447, 4794
Bse21I CCTNAGG 2 cut(s) 99, 4695
Bse3DI GCAATG 3 cut(s) 707, 1425, 2939
Bse8I GATNNNNATC 1 cut(s) 663
BseBI CCWGG 8 cut(s) 614, 1371, 2185, 2346, 3254, 4045, 4460, 4774
BseCI ATCGAT 2 cut(s) 657, 3876
BseDI CCNNGG 8 cut(s) 248, 924, 1875, 1891, 2848, 3252, 3293, 4044
BseJI GATNNNNATC 1 cut(s) 663
BseMI GCAATG 3 cut(s) 707, 1425, 2939
BseNI ACTGG 7 cut(s) 1985, 2148, 2205, 3070, 3369, 3447, 4794
BseRI GAGGAG 8 cut(s) 116, 705, 1157, 1544, 3644, 3980, 4260, 4712
BseSI GKGCMC 1 cut(s) 2497
BseXI GCAGC 5 cut(s) 661, 1029, 1626, 1867, 2251
BseYI CCCAGC 2 cut(s) 1767, 2794
BsgI GTGCAG 2 cut(s) 1497, 3844
Bsh1236I CGCG 1 cut(s) 932
BshNI GGYRCC 3 cut(s) 2562, 2703, 4611
BshVI ATCGAT 2 cut(s) 657, 3876
BsiHKAI GWGCWC 2 cut(s) 3024, 4563
BsiHKCI CYCGRG 3 cut(s) 807, 3294, 3980
BslFI GGGAC 6 cut(s) 798, 1419, 2866, 4062, 4229, 4716
BsmAI GTCTC 8 cut(s) 686, 881, 1810, 2117, 2265, 2849, 3242, 3582
BsmBI CGTCTC 1 cut(s) 686
BsmFI GGGAC 6 cut(s) 798, 1419, 2866, 4062, 4229, 4716
BsmI GAATGC 4 cut(s) 226, 544, 1790, 1977
Bso31I GGTCTC 2 cut(s) 2117, 3242
BsoBI CYCGRG 3 cut(s) 807, 3294, 3980
Bsp119I TTCGAA 1 cut(s) 4432
Bsp1286I GDGCHC 3 cut(s) 2497, 3024, 4563
Bsp1407I TGTACA 1 cut(s) 2619
Bsp19I CCATGG 1 cut(s) 248
BspACI CCGC 9 cut(s) 498, 932, 1238, 1659, 2401, 2560, 2701, 3423, 3820
BspDI ATCGAT 2 cut(s) 657, 3876
BspFNI CGCG 1 cut(s) 932
BspLI GGNNCC 6 cut(s) 1094, 2564, 2705, 3930, 4598, 4613
BspMAI CTGCAG 4 cut(s) 1644, 2269, 2677, 4770
BspMI ACCTGC 1 cut(s) 1385
BspOI GCTAGC 2 cut(s) 4043, 4685
BspPI GGATC 6 cut(s) 330, 672, 1716, 2083, 3923, 3936
BspQI GCTCTTC 1 cut(s) 339
BspT104I TTCGAA 1 cut(s) 4432
BspT107I GGYRCC 3 cut(s) 2562, 2703, 4611
BspTNI GGTCTC 2 cut(s) 2117, 3242
BsrBI CCGCTC 1 cut(s) 3820
BsrDI GCAATG 3 cut(s) 707, 1425, 2939
BsrGI TGTACA 1 cut(s) 2619
BsrI ACTGG 7 cut(s) 1985, 2148, 2205, 3070, 3369, 3447, 4794
BssECI CCNNGG 8 cut(s) 248, 924, 1875, 1891, 2848, 3252, 3293, 4044
BssSI CACGAG 1 cut(s) 1692
BssT1I CCWWGG 5 cut(s) 248, 924, 1875, 1891, 2848
Bst2BI CACGAG 1 cut(s) 1692
Bst2UI CCWGG 8 cut(s) 614, 1371, 2185, 2346, 3254, 4045, 4460, 4774
Bst6I CTCTTC 4 cut(s) 339, 879, 2020, 4662
BstAPI GCANNNNNTGC 1 cut(s) 2672
BstAUI TGTACA 1 cut(s) 2619
BstBI TTCGAA 1 cut(s) 4432
BstDSI CCRYGG 1 cut(s) 248
BstENI CCTNNNNNAGG 1 cut(s) 4777
BstFNI CGCG 1 cut(s) 932
BstHHI GCGC 2 cut(s) 213, 1047
BstMAI GTCTC 8 cut(s) 686, 881, 1810, 2117, 2265, 2849, 3242, 3582
BstMWI GCNNNNNNNGC 8 cut(s) 365, 432, 1042, 1237, 2672, 2805, 2906, 3019
BstNI CCWGG 8 cut(s) 614, 1371, 2185, 2346, 3254, 4045, 4460, 4774
BstNSI RCATGY 8 cut(s) 330, 1070, 1617, 1753, 1790, 2278, 3098, 4330
BstPAI GACNNNNGTC 1 cut(s) 3148
BstSCI CCNGG 8 cut(s) 612, 1369, 2183, 2344, 3252, 4043, 4458, 4772
BstSFI CTRYAG 6 cut(s) 463, 1640, 2265, 2673, 3186, 4766
BstSLI GKGCMC 1 cut(s) 2497
BstUI CGCG 1 cut(s) 932
BstV1I GCAGC 5 cut(s) 661, 1029, 1626, 1867, 2251
BstV2I GAAGAC 7 cut(s) 29, 1396, 3144, 3452, 3731, 4337, 4657
BstX2I RGATCY 4 cut(s) 335, 664, 1721, 3928
BstXI CCANNNNNNTGG 1 cut(s) 422
BstYI RGATCY 4 cut(s) 335, 664, 1721, 3928
Bsu15I ATCGAT 2 cut(s) 657, 3876
Bsu36I CCTNAGG 2 cut(s) 99, 4695
BsuI GTATCC 1 cut(s) 576
BsuTUI ATCGAT 2 cut(s) 657, 3876
BtgI CCRYGG 1 cut(s) 248
BtgZI GCGATG 1 cut(s) 3548
BtsI GCAGTG 2 cut(s) 2666, 4749
BtsIMutI CAGTG 8 cut(s) 9, 1978, 2666, 2777, 3024, 3195, 4339, 4749
BveI ACCTGC 1 cut(s) 1385
CfoI GCGC 2 cut(s) 213, 1047
Cfr13I GGNCC 8 cut(s) 293, 425, 604, 1093, 1262, 2202, 2831, 4312
ClaI ATCGAT 2 cut(s) 657, 3876
CseI GACGC 2 cut(s) 938, 3100
CspCI CAANNNNNGTGG 2 cut(s) 1052, 1087
DraIII CACNNNGTG 1 cut(s) 1520
EaeI YGGCCR 2 cut(s) 646, 3424
Eam1104I CTCTTC 4 cut(s) 339, 879, 2020, 4662
EarI CTCTTC 4 cut(s) 339, 879, 2020, 4662
EciI GGCGGA 1 cut(s) 1674
Eco130I CCWWGG 5 cut(s) 248, 924, 1875, 1891, 2848
Eco147I AGGCCT 1 cut(s) 368
Eco31I GGTCTC 2 cut(s) 2117, 3242
Eco32I GATATC 3 cut(s) 75, 1170, 4507
Eco47I GGWCC 2 cut(s) 2202, 2831
Eco57I CTGAAG 4 cut(s) 2570, 3743, 4331, 4651
Eco81I CCTNAGG 2 cut(s) 99, 4695
Eco88I CYCGRG 3 cut(s) 807, 3294, 3980
EcoNI CCTNNNNNAGG 1 cut(s) 4777
EcoO109I RGGNCCY 3 cut(s) 1093, 2831, 4312
EcoRI GAATTC 2 cut(s) 203, 1129
EcoRII CCWGG 8 cut(s) 612, 1369, 2183, 2344, 3252, 4043, 4458, 4772
EcoRV GATATC 3 cut(s) 75, 1170, 4507
EcoT14I CCWWGG 5 cut(s) 248, 924, 1875, 1891, 2848
EcoT22I ATGCAT 3 cut(s) 246, 1792, 2235
ErhI CCWWGG 5 cut(s) 248, 924, 1875, 1891, 2848
Esp3I CGTCTC 1 cut(s) 686
FalI AAGNNNNNCTT 4 cut(s) 899, 931, 3575, 3607
FaqI GGGAC 6 cut(s) 798, 1419, 2866, 4062, 4229, 4716
FauNDI CATATG 1 cut(s) 4818
FbaI TGATCA 1 cut(s) 1321
FblI GTMKAC 1 cut(s) 3185
Fnu4HI GCNGC 8 cut(s) 675, 1043, 1238, 1640, 1881, 2265, 2702, 3424
Fsp4HI GCNGC 8 cut(s) 675, 1043, 1238, 1640, 1881, 2265, 2702, 3424
FspI TGCGCA 1 cut(s) 1046
GlaI GCGC 2 cut(s) 212, 1046
GluI GCNGC 8 cut(s) 675, 1043, 1238, 1640, 1881, 2265, 2702, 3424
GsaI CCCAGC 2 cut(s) 1771, 2798
GsuI CTGGAG 4 cut(s) 596, 3275, 3386, 4252
HgaI GACGC 2 cut(s) 938, 3100
HhaI GCGC 2 cut(s) 213, 1047
Hin6I GCGC 2 cut(s) 211, 1045
HinP1I GCGC 2 cut(s) 211, 1045
HincII GTYRAC 2 cut(s) 2607, 4114
HindII GTYRAC 2 cut(s) 2607, 4114
HindIII AAGCTT 3 cut(s) 1825, 3404, 3599
HpaI GTTAAC 1 cut(s) 4114
HphI GGTGA 6 cut(s) 283, 586, 2258, 3935, 4070, 4180
Hpy166II GTNNAC 6 cut(s) 2138, 2607, 3116, 3186, 4114, 4749
Hpy8I GTNNAC 6 cut(s) 2138, 2607, 3116, 3186, 4114, 4749
HpyCH4IV ACGT 6 cut(s) 1050, 2993, 3457, 3673, 3858, 4110
HpyF10VI GCNNNNNNNGC 8 cut(s) 365, 432, 1042, 1237, 2672, 2805, 2906, 3019
HpySE526I ACGT 6 cut(s) 1050, 2993, 3457, 3673, 3858, 4110
HspAI GCGC 2 cut(s) 211, 1045
KpnI GGTACC 1 cut(s) 2566
Ksp22I TGATCA 1 cut(s) 1321
KspAI GTTAAC 1 cut(s) 4114
LguI GCTCTTC 1 cut(s) 339
LmnI GCTCC 9 cut(s) 356, 476, 597, 1661, 2173, 3538, 3631, 4387, 4566
Lsp1109I GCAGC 5 cut(s) 661, 1029, 1626, 1867, 2251
MaeII ACGT 6 cut(s) 1050, 2993, 3457, 3673, 3858, 4110
MbiI CCGCTC 1 cut(s) 3820
MfeI CAATTG 3 cut(s) 1389, 3428, 3441
MflI RGATCY 4 cut(s) 335, 664, 1721, 3928
MhlI GDGCHC 3 cut(s) 2497, 3024, 4563
MlsI TGGCCA 1 cut(s) 648
MluNI TGGCCA 1 cut(s) 648
MlyI GAGTC 8 cut(s) 19, 1561, 1896, 1966, 2612, 3558, 4476, 4698
MmeI TCCRAC 3 cut(s) 3084, 4280, 4482
Mox20I TGGCCA 1 cut(s) 648
Mph1103I ATGCAT 3 cut(s) 246, 1792, 2235
MroXI GAANNNNTTC 3 cut(s) 113, 4519, 4656
MscI TGGCCA 1 cut(s) 648
MslI CAYNNNNRTG 7 cut(s) 420, 821, 2131, 3504, 3643, 3733, 3747
Msp20I TGGCCA 1 cut(s) 648
MspA1I CMGCKG 1 cut(s) 4671
MspR9I CCNGG 8 cut(s) 614, 1371, 2185, 2346, 3254, 4045, 4460, 4774
MunI CAATTG 3 cut(s) 1389, 3428, 3441
Mva1269I GAATGC 4 cut(s) 226, 544, 1790, 1977
MvaI CCWGG 8 cut(s) 614, 1371, 2185, 2346, 3254, 4045, 4460, 4774
MvnI CGCG 1 cut(s) 932
MwoI GCNNNNNNNGC 8 cut(s) 365, 432, 1042, 1237, 2672, 2805, 2906, 3019
NcoI CCATGG 1 cut(s) 248
NdeI CATATG 1 cut(s) 4818
NheI GCTAGC 2 cut(s) 4039, 4681
NlaIV GGNNCC 6 cut(s) 1094, 2564, 2705, 3930, 4598, 4613
NmeAIII GCCGAG 1 cut(s) 1266
NsbI TGCGCA 1 cut(s) 1046
NsiI ATGCAT 3 cut(s) 246, 1792, 2235
NspI RCATGY 8 cut(s) 330, 1070, 1617, 1753, 1790, 2278, 3098, 4330
NspV TTCGAA 1 cut(s) 4432
PaeI GCATGC 2 cut(s) 330, 1753
PaeR7I CTCGAG 1 cut(s) 3980
PceI AGGCCT 1 cut(s) 368
PciI ACATGT 2 cut(s) 1613, 2274
PciSI GCTCTTC 1 cut(s) 339
PctI GAATGC 4 cut(s) 226, 544, 1790, 1977
PdmI GAANNNNTTC 3 cut(s) 113, 4519, 4656
PflFI GACNNNGTC 1 cut(s) 182
PflMI CCANNNNNTGG 3 cut(s) 1376, 1443, 3433
PfoI TCCNGGA 2 cut(s) 612, 4458
PkrI GCNGC 8 cut(s) 676, 1044, 1239, 1641, 1882, 2266, 2703, 3425
PleI GAGTC 8 cut(s) 19, 1560, 1895, 1965, 2611, 3558, 4476, 4698
PpsI GAGTC 8 cut(s) 19, 1560, 1895, 1965, 2611, 3558, 4476, 4698
PpuMI RGGWCCY 1 cut(s) 2831
PscI ACATGT 2 cut(s) 1613, 2274
PshAI GACNNNNGTC 1 cut(s) 3148
PsiI TTATAA 2 cut(s) 3453, 3836
Psp1406I AACGTT 1 cut(s) 2993
Psp5II RGGWCCY 1 cut(s) 2831
Psp6I CCWGG 8 cut(s) 612, 1369, 2183, 2344, 3252, 4043, 4458, 4772
PspFI CCCAGC 2 cut(s) 1767, 2794
PspGI CCWGG 8 cut(s) 612, 1369, 2183, 2344, 3252, 4043, 4458, 4772
PspN4I GGNNCC 6 cut(s) 1094, 2564, 2705, 3930, 4598, 4613
PspPI GGNCC 8 cut(s) 293, 425, 604, 1093, 1262, 2202, 2831, 4312
PspPPI RGGWCCY 1 cut(s) 2831
PspXI VCTCGAGB 1 cut(s) 3980
PstI CTGCAG 4 cut(s) 1644, 2269, 2677, 4770
PsuI RGATCY 4 cut(s) 335, 664, 1721, 3928
PsyI GACNNNGTC 1 cut(s) 182
PvuII CAGCTG 1 cut(s) 4671
RseI CAYNNNNRTG 7 cut(s) 420, 821, 2131, 3504, 3643, 3733, 3747
SapI GCTCTTC 1 cut(s) 339
SatI GCNGC 8 cut(s) 675, 1043, 1238, 1640, 1881, 2265, 2702, 3424
Sau96I GGNCC 8 cut(s) 293, 425, 604, 1093, 1262, 2202, 2831, 4312
ScaI AGTACT 3 cut(s) 2486, 2785, 3986
SchI GAGTC 8 cut(s) 19, 1561, 1896, 1966, 2612, 3558, 4476, 4698
ScrFI CCNGG 8 cut(s) 614, 1371, 2185, 2346, 3254, 4045, 4460, 4774
SduI GDGCHC 3 cut(s) 2497, 3024, 4563
SfcI CTRYAG 6 cut(s) 463, 1640, 2265, 2673, 3186, 4766
Sfr274I CTCGAG 1 cut(s) 3980
SfuI TTCGAA 1 cut(s) 4432
SinI GGWCC 2 cut(s) 2202, 2831
SlaI CTCGAG 1 cut(s) 3980
SmiMI CAYNNNNRTG 7 cut(s) 420, 821, 2131, 3504, 3643, 3733, 3747
SmlI CTYRAG 8 cut(s) 194, 2488, 2866, 3206, 3980, 3988, 4424, 4467
SmoI CTYRAG 8 cut(s) 194, 2488, 2866, 3206, 3980, 3988, 4424, 4467
SpeI ACTAGT 2 cut(s) 3620, 4528
SphI GCATGC 2 cut(s) 330, 1753
SseBI AGGCCT 1 cut(s) 368
SsiI CCGC 9 cut(s) 498, 932, 1238, 1659, 2401, 2560, 2701, 3423, 3820
SspI AATATT 2 cut(s) 81, 1938
StuI AGGCCT 1 cut(s) 368
StyD4I CCNGG 8 cut(s) 612, 1369, 2183, 2344, 3252, 4043, 4458, 4772
StyI CCWWGG 5 cut(s) 248, 924, 1875, 1891, 2848
TaiI ACGT 6 cut(s) 1053, 2996, 3460, 3676, 3861, 4113
TatI WGTACW 5 cut(s) 710, 2484, 2619, 2783, 3984
TauI GCSGC 3 cut(s) 1240, 2704, 3426
TscAI CASTG 8 cut(s) 16, 1978, 2673, 2784, 3031, 3195, 4346, 4756
TseI GCWGC 5 cut(s) 674, 1042, 1639, 1880, 2264
TspRI CASTG 8 cut(s) 16, 1978, 2673, 2784, 3031, 3195, 4346, 4756
Tth111I GACNNNGTC 1 cut(s) 182
Van91I CCANNNNNTGG 3 cut(s) 1376, 1443, 3433
VpaK11BI GGWCC 2 cut(s) 2202, 2831
XagI CCTNNNNNAGG 1 cut(s) 4777
XapI RAATTY 7 cut(s) 203, 1129, 1542, 2020, 2369, 2378, 3474
XbaI TCTAGA 2 cut(s) 1103, 4390
XceI RCATGY 8 cut(s) 330, 1070, 1617, 1753, 1790, 2278, 3098, 4330
XhoI CTCGAG 1 cut(s) 3980
XmiI GTMKAC 1 cut(s) 3185
XmnI GAANNNNTTC 3 cut(s) 113, 4519, 4656
ZrmI AGTACT 3 cut(s) 2486, 2785, 3986
Zsp2I ATGCAT 3 cut(s) 246, 1792, 2235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.