FvH4_7g25671

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000070
Physical Location & Seq
Forward (+)
19597360 .. 19599300
1941 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g25671.t1

Sequence Viewer

Length: 1338 bp
ATGGTAATTTCGGTGTCCGCAGGTATAGCTGGAATCAGATCGATCTGCAACAAGAAAGTCTGTCATGATCATGATCTAAGAGACTTGGCTCACCTTGTGGAAGAGTTAACGTTGCAAGTTTTTGAATACTATCGATTGTACAAAAAAGAAACGATGGGGGAGGCTGTTCTTGGTGCATTCCTCCCGGTGCTGCTTGAGAAGTTGGCGGATCAAGAGATCACTGAGTACTTCGGACGCCTTAAGGGGGTCGACAAAAAGGTGCTGGAGAAATGGACTAGAACGTTGACGGCCATTGAAGCGGTGCTGAGTGATGCCGAGGAGAAGCAACTAACACAAAGAGCAGTAAAAGAGTGGCTGGATGATCTCAGAGATTTGGCTTACGATGTTCAAGATGTATTGGACATATTTGATACTAAAATGCTGAAGCGGAGGATTGAAAAGAAACAAGGAAGCAAAAGCAAGTTATGGAGCTCACTCTCTAAAGTTAAGTCCAATTTTGGTCTGAACTCAGCAATCAAGAAGATAAGTGATCGACTTGAGGAGATAAGTACTCGAGAGAAGCAGTTTGGTTTGAAGAAACTTGGAGTGTCTAAAAAGCCATGGAAAATGCCACCCACTACTTATCAACTTGATGGACCTGTGATCGGAAGGAATGAAGCAAAGGCTAAGATTCTCTATGATTTGCTTTCAAAACAAGAGAATTATCATGTGGTTGGTATTGTCGGTACTGGAGGACTAGGAAAGAAAACACTTGCTAAACATGTATTCAACGATGCTGCAACCGACCAGTTTTCCCCAAAAGGATGGGTATCTGTGTCAGACGACTTTGACATTGTAAGGGTGGCAGCAGCCGTTTTGGAATCAGTCACGTCCGCTCCAGTTCAGGAATTCAAGGAGCTTAACTCAATTCTGGAGAAGTTAAGTAAGGAATTGGCTAGCAAGAAGTTTTTAATTGTTCTTGATGATGTCTGGGACACTTGTAGCTATGGTCAATGGACTACACTGCAGGCCTCCTTTCGTGTTGGAGCAGCTGGAAGTAAGATCATCGTGACAACACGTGACGCTAATGTCGCAAAGATGATGGGAGATAGTAGCCCTTATAACTTGAAGCCGATGTCAGAAGATGATTGTTGGAAACTCTTTCAGCACCATGCACTCTTAAATAACAGACCTGAAGATGTTGAGTTGCTTAAGGAGAAAATTGTAGAAAAATGTGATGGGTTGCCACTCAGAACAGAACAAGATGGAGGATATCCTTCCGGCATTAAAACTGAGCTATCATTATCTCCCTTCGACTTTGAAACGGTGTTTCTCCTACTGTGGAATACTTCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

446

Amino Acids

50.14

Weight (kDa)

7.58

Isoelectric Point (pI)

36.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 61 - 150 4.4e-21 Rx N-terminal domain
NB-ARC PF00931 223 - 386 1.4e-35 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1101
AasI GACNNNNNNGTC 1 cut(s) 1067
Acc36I ACCTGC 1 cut(s) 11
AccBSI CCGCTC 1 cut(s) 875
AccI GTMKAC 1 cut(s) 249
AciI CCGC 5 cut(s) 18, 206, 299, 427, 873
AclI AACGTT 2 cut(s) 110, 281
AclWI GGATC 1 cut(s) 216
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 1 cut(s) 887
AcuI CTGAAG 2 cut(s) 443, 1194
AcvI CACGTG 1 cut(s) 1058
AcyI GRCGYC 1 cut(s) 235
AdeI CACNNNGTG 1 cut(s) 97
AfaI GTAC 4 cut(s) 140, 227, 550, 727
AfiI CCNNNNNNNGG 2 cut(s) 244, 644
AflII CTTAAG 2 cut(s) 239, 1190
AflIII ACRYGT 2 cut(s) 760, 1055
AjiI CACGTC 1 cut(s) 870
AluBI AGCT 6 cut(s) 29, 471, 898, 984, 1031, 1276
AluI AGCT 6 cut(s) 29, 471, 898, 984, 1031, 1276
Alw21I GWGCWC 1 cut(s) 473
Alw26I GTCTC 1 cut(s) 75
AlwI GGATC 1 cut(s) 216
Ama87I CYCGRG 1 cut(s) 552
AoxI GGCC 2 cut(s) 288, 1008
ApeKI GCWGC 5 cut(s) 190, 776, 845, 848, 1028
ApoI RAATTY 1 cut(s) 887
AspS9I GGNCC 1 cut(s) 635
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 83
AsuNHI GCTAGC 1 cut(s) 935
AvaI CYCGRG 1 cut(s) 552
AvaII GGWCC 1 cut(s) 635
BanII GRGCYC 1 cut(s) 473
BbrPI CACGTG 1 cut(s) 1058
Bbv12I GWGCWC 1 cut(s) 473
BbvI GCAGC 5 cut(s) 177, 763, 857, 860, 1040
BccI CCATC 6 cut(s) 148, 626, 798, 1075, 1211, 1238
BceAI ACGGC 2 cut(s) 303, 836
BclI TGATCA 1 cut(s) 67
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 1 cut(s) 75
BfaI CTAG 3 cut(s) 276, 737, 936
BfmI CTRYAG 1 cut(s) 1004
BfrI CTTAAG 2 cut(s) 239, 1190
BfuAI ACCTGC 1 cut(s) 11
BisI GCNGC 5 cut(s) 191, 777, 846, 849, 1029
BlsI GCNGC 5 cut(s) 192, 778, 847, 850, 1030
BmcAI AGTACT 2 cut(s) 227, 550
Bme1390I CCNGG 1 cut(s) 185
Bme18I GGWCC 1 cut(s) 635
BmeT110I CYCGRG 1 cut(s) 552
BmgBI CACGTC 1 cut(s) 870
BmgT120I GGNCC 1 cut(s) 635
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 2 cut(s) 301, 763
BmtI GCTAGC 1 cut(s) 939
BplI GAGNNNNNCTC 2 cut(s) 887, 919
BpmI CTGGAG 4 cut(s) 284, 750, 861, 932
BpuEI CTTGAG 2 cut(s) 215, 557
BpuMI CCSGG 1 cut(s) 185
Bsa29I ATCGAT 2 cut(s) 41, 133
BsaAI YACGTR 1 cut(s) 1058
BsaBI GATNNNNATC 2 cut(s) 72, 808
BsaHI GRCGYC 1 cut(s) 235
BsaJI CCNNGG 2 cut(s) 315, 599
BsaXI ACNNNNNCTCC 2 cut(s) 859, 889
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 644
Bse1I ACTGG 3 cut(s) 733, 787, 878
Bse8I GATNNNNATC 2 cut(s) 72, 808
BseCI ATCGAT 2 cut(s) 41, 133
BseDI CCNNGG 2 cut(s) 315, 599
BseGI GGATG 2 cut(s) 364, 809
BseJI GATNNNNATC 2 cut(s) 72, 808
BseLI CCNNNNNNNGG 2 cut(s) 244, 644
BseMII CTCAG 6 cut(s) 213, 296, 379, 522, 1243, 1263
BseNI ACTGG 3 cut(s) 733, 787, 878
BseRI GAGGAG 2 cut(s) 332, 554
BseXI GCAGC 5 cut(s) 177, 763, 857, 860, 1040
BshFI GGCC 2 cut(s) 290, 1010
BshVI ATCGAT 2 cut(s) 41, 133
BsiHKAI GWGCWC 1 cut(s) 473
BsiHKCI CYCGRG 1 cut(s) 552
BsiSI CCGG 2 cut(s) 185, 1260
BslFI GGGAC 1 cut(s) 986
BslI CCNNNNNNNGG 2 cut(s) 244, 644
BsmAI GTCTC 1 cut(s) 75
BsmFI GGGAC 1 cut(s) 986
BsmI GAATGC 1 cut(s) 176
BsnI GGCC 2 cut(s) 290, 1010
BsoBI CYCGRG 1 cut(s) 552
Bsp1286I GDGCHC 1 cut(s) 473
Bsp1407I TGTACA 1 cut(s) 138
Bsp19I CCATGG 1 cut(s) 599
BspACI CCGC 5 cut(s) 18, 206, 299, 427, 873
BspANI GGCC 2 cut(s) 290, 1010
BspCNI CTCAG 6 cut(s) 214, 297, 378, 521, 1242, 1264
BspDI ATCGAT 2 cut(s) 41, 133
BspHI TCATGA 2 cut(s) 64, 70
BspMAI CTGCAG 1 cut(s) 1008
BspMI ACCTGC 1 cut(s) 11
BspOI GCTAGC 1 cut(s) 939
BspPI GGATC 1 cut(s) 216
BspTI CTTAAG 2 cut(s) 239, 1190
BsrBI CCGCTC 1 cut(s) 875
BsrGI TGTACA 1 cut(s) 138
BsrI ACTGG 3 cut(s) 733, 787, 878
BssECI CCNNGG 2 cut(s) 315, 599
BssNI GRCGYC 1 cut(s) 235
BssT1I CCWWGG 1 cut(s) 599
Bst4CI ACNGT 2 cut(s) 1306, 1320
Bst6I CTCTTC 1 cut(s) 96
BstACI GRCGYC 1 cut(s) 235
BstAFI CTTAAG 2 cut(s) 239, 1190
BstAUI TGTACA 1 cut(s) 138
BstBAI YACGTR 1 cut(s) 1058
BstC8I GCNNGC 2 cut(s) 937, 1008
BstDEI CTNAG 8 cut(s) 77, 222, 305, 365, 508, 666, 1229, 1272
BstDSI CCRYGG 1 cut(s) 599
BstF5I GGATG 2 cut(s) 364, 809
BstMAI GTCTC 1 cut(s) 75
BstMWI GCNNNNNNNGC 3 cut(s) 26, 296, 1070
BstNSI RCATGY 1 cut(s) 764
BstSCI CCNGG 1 cut(s) 183
BstSFI CTRYAG 1 cut(s) 1004
BstV1I GCAGC 5 cut(s) 177, 763, 857, 860, 1040
BstXI CCANNNNNNTGG 1 cut(s) 804
Bsu15I ATCGAT 2 cut(s) 41, 133
BsuRI GGCC 2 cut(s) 290, 1010
BsuTUI ATCGAT 2 cut(s) 41, 133
BtgI CCRYGG 1 cut(s) 599
BtrI CACGTC 1 cut(s) 870
BtsCI GGATG 2 cut(s) 364, 809
BtsI GCAGTG 1 cut(s) 1001
BtsIMutI CAGTG 2 cut(s) 219, 1001
BveI ACCTGC 1 cut(s) 11
Cac8I GCNNGC 2 cut(s) 937, 1008
CciI TCATGA 2 cut(s) 64, 70
Cfr13I GGNCC 1 cut(s) 635
ClaI ATCGAT 2 cut(s) 41, 133
CseI GACGC 2 cut(s) 243, 1070
Csp6I GTAC 4 cut(s) 139, 226, 549, 726
CviAII CATG 6 cut(s) 65, 71, 600, 707, 761, 1151
CviQI GTAC 4 cut(s) 139, 226, 549, 726
DdeI CTNAG 8 cut(s) 77, 222, 305, 365, 508, 666, 1229, 1272
DraIII CACNNNGTG 1 cut(s) 97
DrdI GACNNNNNNGTC 1 cut(s) 1067
DseDI GACNNNNNNGTC 1 cut(s) 1067
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
EciI GGCGGA 1 cut(s) 221
Ecl136II GAGCTC 1 cut(s) 471
Eco130I CCWWGG 1 cut(s) 599
Eco147I AGGCCT 1 cut(s) 1010
Eco24I GRGCYC 1 cut(s) 473
Eco32I GATATC 1 cut(s) 1253
Eco47I GGWCC 1 cut(s) 635
Eco53kI GAGCTC 1 cut(s) 471
Eco57I CTGAAG 2 cut(s) 443, 1194
Eco72I CACGTG 1 cut(s) 1058
Eco88I CYCGRG 1 cut(s) 552
EcoICRI GAGCTC 1 cut(s) 471
EcoRI GAATTC 1 cut(s) 887
EcoRV GATATC 1 cut(s) 1253
EcoT14I CCWWGG 1 cut(s) 599
EcoT38I GRGCYC 1 cut(s) 473
ErhI CCWWGG 1 cut(s) 599
FaeI CATG 6 cut(s) 68, 74, 603, 710, 764, 1154
FaqI GGGAC 1 cut(s) 986
FatI CATG 6 cut(s) 64, 70, 599, 706, 760, 1150
FbaI TGATCA 1 cut(s) 67
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 5 cut(s) 191, 777, 846, 849, 1029
FokI GGATG 2 cut(s) 371, 816
FriOI GRGCYC 1 cut(s) 473
Fsp4HI GCNGC 5 cut(s) 191, 777, 846, 849, 1029
FspBI CTAG 3 cut(s) 276, 737, 936
GluI GCNGC 5 cut(s) 191, 777, 846, 849, 1029
GsuI CTGGAG 4 cut(s) 284, 750, 861, 932
HaeIII GGCC 2 cut(s) 290, 1010
HapII CCGG 2 cut(s) 185, 1260
HgaI GACGC 2 cut(s) 243, 1070
Hin1I GRCGYC 1 cut(s) 235
Hin1II CATG 6 cut(s) 68, 74, 603, 710, 764, 1154
HincII GTYRAC 3 cut(s) 108, 250, 285
HindII GTYRAC 3 cut(s) 108, 250, 285
HinfI GANTC 3 cut(s) 33, 670, 860
HpaI GTTAAC 1 cut(s) 108
HpaII CCGG 2 cut(s) 185, 1260
HphI GGTGA 1 cut(s) 83
Hpy166II GTNNAC 3 cut(s) 108, 250, 285
Hpy188I TCNGA 8 cut(s) 38, 233, 368, 504, 647, 820, 1120, 1232
Hpy8I GTNNAC 3 cut(s) 108, 250, 285
HpyAV CCTTC 3 cut(s) 642, 1266, 1300
HpyCH4III ACNGT 2 cut(s) 1306, 1320
HpyCH4IV ACGT 4 cut(s) 110, 281, 869, 1057
HpyCH4V TGCA 6 cut(s) 48, 115, 176, 779, 1006, 1154
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 296, 1070
HpyF3I CTNAG 8 cut(s) 77, 222, 305, 365, 508, 666, 1229, 1272
HpySE526I ACGT 4 cut(s) 110, 281, 869, 1057
Hsp92I GRCGYC 1 cut(s) 235
Hsp92II CATG 6 cut(s) 68, 74, 603, 710, 764, 1154
Ksp22I TGATCA 1 cut(s) 67
KspAI GTTAAC 1 cut(s) 108
LmnI GCTCC 4 cut(s) 468, 880, 895, 1025
Lsp1109I GCAGC 5 cut(s) 177, 763, 857, 860, 1040
LweI GCATC 2 cut(s) 301, 763
MaeI CTAG 3 cut(s) 276, 737, 936
MaeII ACGT 4 cut(s) 110, 281, 869, 1057
MaeIII GTNAC 3 cut(s) 865, 1048, 1058
MbiI CCGCTC 1 cut(s) 875
MboII GAAGA 5 cut(s) 113, 532, 586, 1133, 1187
MhlI GDGCHC 1 cut(s) 473
MluCI AATT 8 cut(s) 6, 493, 700, 887, 906, 929, 951, 1200
MmeI TCCRAC 2 cut(s) 1003, 1112
MnlI CCTC 8 cut(s) 154, 191, 310, 423, 532, 725, 1021, 1241
MseI TTAA 9 cut(s) 107, 240, 486, 900, 920, 950, 1160, 1191, 1266
MslI CAYNNNNRTG 1 cut(s) 69
MspA1I CMGCKG 1 cut(s) 1031
MspCI CTTAAG 2 cut(s) 239, 1190
MspI CCGG 2 cut(s) 185, 1260
MspR9I CCNGG 1 cut(s) 185
Mva1269I GAATGC 1 cut(s) 176
MwoI GCNNNNNNNGC 3 cut(s) 26, 296, 1070
NciI CCSGG 1 cut(s) 185
NcoI CCATGG 1 cut(s) 599
NheI GCTAGC 1 cut(s) 935
NlaIII CATG 6 cut(s) 68, 74, 603, 710, 764, 1154
NmeAIII GCCGAG 1 cut(s) 340
NmuCI GTSAC 3 cut(s) 865, 1048, 1058
NspI RCATGY 1 cut(s) 764
PaeR7I CTCGAG 1 cut(s) 552
PagI TCATGA 2 cut(s) 64, 70
PceI AGGCCT 1 cut(s) 1010
PciI ACATGT 1 cut(s) 760
PctI GAATGC 1 cut(s) 176
PfeI GAWTC 3 cut(s) 33, 670, 860
PkrI GCNGC 5 cut(s) 192, 778, 847, 850, 1030
PmaCI CACGTG 1 cut(s) 1058
PmlI CACGTG 1 cut(s) 1058
Ppu21I YACGTR 1 cut(s) 1058
PscI ACATGT 1 cut(s) 760
PsiI TTATAA 1 cut(s) 1101
Psp124BI GAGCTC 1 cut(s) 473
Psp1406I AACGTT 2 cut(s) 110, 281
PspCI CACGTG 1 cut(s) 1058
PspPI GGNCC 1 cut(s) 635
PstI CTGCAG 1 cut(s) 1008
PvuII CAGCTG 1 cut(s) 1031
RsaI GTAC 4 cut(s) 140, 227, 550, 727
RsaNI GTAC 4 cut(s) 139, 226, 549, 726
RseI CAYNNNNRTG 1 cut(s) 69
SacI GAGCTC 1 cut(s) 473
SalI GTCGAC 1 cut(s) 248
SaqAI TTAA 9 cut(s) 107, 240, 486, 900, 920, 950, 1160, 1191, 1266
SatI GCNGC 5 cut(s) 191, 777, 846, 849, 1029
Sau96I GGNCC 1 cut(s) 635
ScaI AGTACT 2 cut(s) 227, 550
ScrFI CCNGG 1 cut(s) 185
SduI GDGCHC 1 cut(s) 473
SfaNI GCATC 2 cut(s) 301, 763
SfcI CTRYAG 1 cut(s) 1004
Sfr274I CTCGAG 1 cut(s) 552
SinI GGWCC 1 cut(s) 635
SlaI CTCGAG 1 cut(s) 552
SmiMI CAYNNNNRTG 1 cut(s) 69
SmlI CTYRAG 5 cut(s) 194, 239, 536, 552, 1190
SmoI CTYRAG 5 cut(s) 194, 239, 536, 552, 1190
Sse9I AATT 8 cut(s) 6, 493, 700, 887, 906, 929, 951, 1200
SseBI AGGCCT 1 cut(s) 1010
SsiI CCGC 5 cut(s) 18, 206, 299, 427, 873
SspMI CTAG 3 cut(s) 276, 737, 936
SstI GAGCTC 1 cut(s) 473
StuI AGGCCT 1 cut(s) 1010
StyD4I CCNGG 1 cut(s) 183
StyI CCWWGG 1 cut(s) 599
TaaI ACNGT 2 cut(s) 1306, 1320
TaiI ACGT 4 cut(s) 113, 284, 872, 1060
TaqI TCGA 6 cut(s) 41, 133, 249, 532, 553, 1293
TasI AATT 8 cut(s) 6, 493, 700, 887, 906, 929, 951, 1200
TatI WGTACW 3 cut(s) 138, 225, 548
TfiI GAWTC 3 cut(s) 33, 670, 860
Tru1I TTAA 9 cut(s) 107, 240, 486, 900, 920, 950, 1160, 1191, 1266
Tru9I TTAA 9 cut(s) 107, 240, 486, 900, 920, 950, 1160, 1191, 1266
TscAI CASTG 2 cut(s) 226, 1008
TseFI GTSAC 3 cut(s) 865, 1048, 1058
TseI GCWGC 5 cut(s) 190, 776, 845, 848, 1028
Tsp45I GTSAC 3 cut(s) 865, 1048, 1058
TspDTI ATGAA 1 cut(s) 669
TspRI CASTG 2 cut(s) 226, 1008
Vha464I CTTAAG 2 cut(s) 239, 1190
VpaK11BI GGWCC 1 cut(s) 635
XapI RAATTY 1 cut(s) 887
XceI RCATGY 1 cut(s) 764
XhoI CTCGAG 1 cut(s) 552
XmiI GTMKAC 1 cut(s) 249
XspI CTAG 3 cut(s) 276, 737, 936
ZrmI AGTACT 2 cut(s) 227, 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.