RchiOBHm_Chr1g0320281

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000070
Physical Location & Seq
Reverse (-)
7931044 .. 7934286
3243 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55045

Sequence Viewer

Length: 2796 bp
ATGTTAGAACGCGAGCAGCTCAAGCTTCACCAAACTGGCACAAGCAAGGTACGAGGCTTCTTTACCAAAGTTCCTCACAAAGTTAAATTCAACTTCAATATGAACTCTGAAATAGACGACATTGCTAAACGCTTGCAAGACATATTTGACCGGAAAGAGAAACTCGGTTTGAAATATATTGAGCATACCCCTACTTCAACATCATCATCGCACAGGACACCAAGTTCATATGTGTTGGATGGATCTGTGGTTGGGAGAGATGAAGACGCAGGAAAGATTGTGGAATTGTTGTCCAGAAATGCTGATCCTTCTTCTCCCACAAATTATCAAGTTGTTGCCATTGTTGGTATGGGTGGACTCGGCAAGACAACGCTTGCGGGACGAGTCTTCAATGATGTAGTTGCAATGAAACAGTTTGATCTCAAGATCTGGGTTTCAGTATCCGATAACTTTGATCTTCAAACGGTGACGAGAGCTATTTTTAAGGAAGTTACATCTCGGCCCTGTGATATGGATGAGTTCAGTCGACTTCAAGATAATCTGAGCAAGAAGATTGATGGTAAAAGGTTTTTGATTGTTTTAGATGATGTCTGGAGCACATGTGATTATGATTCATGGACAAAACTGCAAGCTCCCCTCCGTGGCGGAGCCAAGGGAAGTAAGGTAATGGTGACAACACGTGACGAAAAAGTTGCAATATTGATGGGAGCCCCAGCCGGTGATGTTTATCATTTAAAGACTCTATCAGATGAAAGTTGTTTGCAAGTATTTGAGCAGCATGTTAGCAATGACAGGCCACCGAATTTTGAGTTGCTTAAGGAGAAAATTGTTACGAATTGCAATGGATTGCCATTGGCTGCAAAAACTCTTGGTGGTGTTTTACGTTGTGAAGAAACTGGCAAGTGGGAGGAAATACTGAATGACAAATTATGGAGTATGTCAGATAGGAGTAAGATACTCCCAGTACTGAGATTGAGTTATCATTATCTCCCTTCACCTTTGAAGAGGTGCTTTGCCTATTGCTCAATACTTCCCAATGACTATGAATTTGGGAAAACCCAATTAATCCTTCTGTGGATGGCAGAGGGTTTTCTTGAGCAACCAAAAGGGACTAAAGTAATGGAAGATATCGGTGACGAATATTTTGGGGAGCTATTGTCTCGGTCATTATTTCAAACCTCGGGCAAAAACAGTTTGTGCTTTGTAATGCATGACCTTGTTGGTGATTTGGCACGATGGGCTGCTGGAGACACATTTTGCAGATTGGAGGATAAACTGCATGGTAGATGTTCTCCGAACACTCGTCATCTGAGTTACATTTCTGCTAAGTTTGATGGGGAAAAAAGATTTGAGACATTTTCTGAAGCAAAAGGCTTGCGGACTTTCCTACCACTTCCCGTTTCCGGTGGTTATGAGAATTATCTGACTCGTTATGCTACTTCTGATTTATTGCCACAATTGAAATACTTGCGGGTGCTCTCCTTCAATGGCTATAAACTGACCGAGGTGCCAAATTCCGTAGGTAAGTTGAGACATCTACGGTACCTTGATCTTTCTCACACATTAATAACGTGTTTGCCTGAGTCAACATGCATGCTTTACAACTTACAGACGTTAATATTAGAGAATTGTTCAAAATTGAAGACATTACCTTCAAACATGAGCAATTTATCTAATTTACGTCATCTCAACAATTCAAACATGCCTTCATTGGAAGAAATGCCTCCCAAAGTAAGTCGGTTGACTCATCTGCGAACTTTGCCTAATTTTGTGGTGGGGAAAGGTAGTGCATCAGGGATTGGAGAGATACAGTCATTGCATCTTCGTGGGACCTTGAGGGTTGGAAGATTGGAAAATGTGATTGATGTTGGGGATGCGAAGACGGCTCAAATAACGAACAAGGAAGGGCTGGAAATCTTGCAATTGGAATGGAGCGGCACAAGCGAGAAGGAGTTGGAGGTGCTGTGCAGTTTAGAACCTCATAAAAAGCTTAAGGAGCTGACCATCAAGGGCTACAATGGTTTCGAATTTTCAAAATGGATTGGACATCCTTCATTCTCTGATATGACGTCTGTGAAATTGGAGAATTGTAAAAACTGTCGATTCTTACCTCCTCTTGGCCAATTACCTTTTCTGAAAAATCTTCAGATACAAGGACTGGTCAATGTTGAAAGTGTGGGTGCTGAGTTTTATGGAGAGTGCAGCTTGCCTTTTCCAGTGTTGGAATGCATGACGTTCCAGGACATGCAAAATTGGAAGGAGTGGCTTCCTTGCAAAAGAGATGAAGAAATTCGAGTTTTCCCATGCATGGAAAAGCTTTCCATCCTTGGTTGCCCCAAATTGAAAGATTGGTTGCCCAAGAGCATGGATTCATTATCAGAGCTGTATATTGCTGCATGTGAAGAGTTAGTGGTTTCAATTGCCAATTATAAACATCTTCGTGGGCTGTTCATCTGCGGTTGTAAGGTGGTGCACAGAAGTGGAGTTAAGTTTGAGCTATTGGAGGATATGTGGCTTGCAAGTATTTCAGAGTTCAGACTTGAAATAGACGGGTTCATCAGAGGATTGCCAAAGCTTCAAAATTTGCGGATTACTGGTTGTGAAGAGTTGACATGTTTATGGGAGAATGAGGATAGATGGCTGCAGCCTCGAATTTCTAATATTATTGGAGGCAACATATCTGACTCCCCCTCTCACTTTATTCAAGAGCTAAGAGCACTCAAGGAACTTGTGCTAACTGGATGCTCAAATCTAATTTCTTTTCCAGAAGTTGGTATCAAGTACCGCCAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

931

Amino Acids

105.4

Weight (kDa)

7.22

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 90 - 263 7e-38 NB-ARC domain
WHD_DRP PF23559 343 - 411 7.8e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 484 - 750 2.3e-23 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 600 - 719 5.6e-34 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2430
AatII GACGTC 1 cut(s) 2072
Acc65I GGTACC 1 cut(s) 1542
AccB1I GGYRCC 2 cut(s) 1507, 1542
AccB7I CCANNNNNTGG 1 cut(s) 2771
AccBSI CCGCTC 1 cut(s) 1935
AccI GTMKAC 1 cut(s) 526
AccII CGCG 1 cut(s) 12
AciI CCGC 8 cut(s) 377, 645, 1378, 1471, 1935, 2457, 2587, 2785
AclWI GGATC 2 cut(s) 250, 299
AcoI YGGCCR 1 cut(s) 2119
AcsI RAATTY 8 cut(s) 86, 802, 1046, 1513, 2027, 2289, 2581, 2652
AcuI CTGAAG 2 cut(s) 1383, 2129
AcvI CACGTG 1 cut(s) 680
AcyI GRCGYC 1 cut(s) 2069
AfaI GTAC 4 cut(s) 51, 966, 1544, 2783
AfiI CCNNNNNNNGG 5 cut(s) 641, 1403, 2117, 2308, 2771
AflII CTTAAG 2 cut(s) 815, 1991
AflIII ACRYGT 4 cut(s) 599, 677, 1571, 2612
AjnI CCWGG 1 cut(s) 2238
AjuI GAANNNNNNNTTGG 4 cut(s) 1053, 1085, 2115, 2147
AleI CACNNNNGTG 1 cut(s) 2478
AloI GAACNNNNNNTCC 2 cut(s) 208, 240
Alw21I GWGCWC 4 cut(s) 599, 1479, 2475, 2719
Alw26I GTCTC 4 cut(s) 1164, 1242, 1346, 1525
Alw44I GTGCAC 1 cut(s) 2471
AlwI GGATC 2 cut(s) 250, 299
Ama87I CYCGRG 1 cut(s) 1180
AoxI GGCC 3 cut(s) 500, 794, 2119
ApaLI GTGCAC 1 cut(s) 2471
ApeKI GCWGC 8 cut(s) 16, 775, 857, 1241, 2202, 2393, 2641, 2644
ApoI RAATTY 8 cut(s) 86, 802, 1046, 1513, 2027, 2289, 2581, 2652
ArsI GACNNNNNNTTYG 4 cut(s) 1031, 1063, 1127, 1159
AseI ATTAAT 2 cut(s) 1064, 1565
Asp700I GAANNNNTTC 1 cut(s) 2289
Asp718I GGTACC 1 cut(s) 1542
AspS9I GGNCC 2 cut(s) 501, 1830
AsuHPI GGTGA 7 cut(s) 20, 478, 682, 731, 987, 1145, 1235
AsuII TTCGAA 1 cut(s) 2025
AvaI CYCGRG 1 cut(s) 1180
AvaII GGWCC 1 cut(s) 1830
BaeGI GKGCMC 1 cut(s) 2475
BalI TGGCCA 1 cut(s) 2121
BanI GGYRCC 2 cut(s) 1507, 1542
BanII GRGCYC 1 cut(s) 712
BbrPI CACGTG 1 cut(s) 680
BbsI GAAGAC 4 cut(s) 270, 379, 1649, 1886
Bbv12I GWGCWC 4 cut(s) 599, 1479, 2475, 2719
BbvI GCAGC 8 cut(s) 28, 787, 844, 1228, 2214, 2380, 2628, 2656
BccI CCATC 9 cut(s) 233, 551, 697, 1072, 1230, 1328, 2012, 2330, 2631
BceAI ACGGC 1 cut(s) 1899
BcgI CGANNNNNNTGC 2 cut(s) 674, 708
BciT130I CCWGG 1 cut(s) 2240
BciVI GTATCC 1 cut(s) 451
BcoDI GTCTC 4 cut(s) 1164, 1242, 1346, 1525
BfmI CTRYAG 1 cut(s) 2642
BfrI CTTAAG 2 cut(s) 815, 1991
BfuI GTATCC 1 cut(s) 451
BglII AGATCT 1 cut(s) 426
BisI GCNGC 9 cut(s) 17, 776, 858, 1242, 1936, 2203, 2394, 2642, 2645
BlsI GCNGC 9 cut(s) 18, 777, 859, 1243, 1937, 2204, 2395, 2643, 2646
BmcAI AGTACT 1 cut(s) 966
Bme1390I CCNGG 1 cut(s) 2240
Bme18I GGWCC 1 cut(s) 1830
BmeT110I CYCGRG 1 cut(s) 1180
BmgT120I GGNCC 2 cut(s) 501, 1830
BmiI GGNNCC 5 cut(s) 649, 709, 1509, 1544, 1831
BmrFI CCNGG 1 cut(s) 2240
BmrI ACTGGG 1 cut(s) 956
BmsI GCATC 4 cut(s) 1799, 1828, 1864, 2732
BmuI ACTGGG 1 cut(s) 956
BpiI GAAGAC 4 cut(s) 270, 379, 1649, 1886
BpmI CTGGAG 2 cut(s) 613, 1266
Bpu14I TTCGAA 1 cut(s) 2025
BpuEI CTTGAG 5 cut(s) 5, 407, 1115, 1855, 2705
BsaAI YACGTR 1 cut(s) 680
BsaBI GATNNNNATC 1 cut(s) 726
BsaHI GRCGYC 1 cut(s) 2069
BsaJI CCNNGG 5 cut(s) 640, 651, 1179, 1503, 2326
BsaWI WCCGGW 2 cut(s) 150, 1403
BsaXI ACNNNNNCTCC 8 cut(s) 586, 616, 1142, 1172, 1949, 1979, 2495, 2525
Bsc4I CCNNNNNNNGG 5 cut(s) 641, 1403, 2117, 2308, 2771
Bse118I RCCGGY 1 cut(s) 716
Bse1I ACTGG 7 cut(s) 40, 901, 962, 2163, 2216, 2599, 2743
Bse3DI GCAATG 5 cut(s) 120, 411, 793, 847, 1814
Bse8I GATNNNNATC 1 cut(s) 726
BseBI CCWGG 1 cut(s) 2240
BseDI CCNNGG 5 cut(s) 640, 651, 1179, 1503, 2326
BseGI GGATG 7 cut(s) 244, 520, 1083, 1879, 2047, 2322, 2747
BseJI GATNNNNATC 1 cut(s) 726
BseLI CCNNNNNNNGG 5 cut(s) 641, 1403, 2117, 2308, 2771
BseMI GCAATG 5 cut(s) 120, 411, 793, 847, 1814
BseMII CTCAG 5 cut(s) 533, 959, 1301, 1572, 2175
BseNI ACTGG 7 cut(s) 40, 901, 962, 2163, 2216, 2599, 2743
BseRI GAGGAG 1 cut(s) 2103
BseSI GKGCMC 1 cut(s) 2475
BseXI GCAGC 8 cut(s) 28, 787, 844, 1228, 2214, 2380, 2628, 2656
BseYI CCCAGC 1 cut(s) 712
BsgI GTGCAG 2 cut(s) 1987, 2221
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 3 cut(s) 502, 796, 2121
BshNI GGYRCC 2 cut(s) 1507, 1542
BsiHKAI GWGCWC 4 cut(s) 599, 1479, 2475, 2719
BsiHKCI CYCGRG 1 cut(s) 1180
BsiSI CCGG 3 cut(s) 151, 717, 1404
BslFI GGGAC 3 cut(s) 393, 1123, 1843
BslI CCNNNNNNNGG 5 cut(s) 641, 1403, 2117, 2308, 2771
BsmAI GTCTC 4 cut(s) 1164, 1242, 1346, 1525
BsmFI GGGAC 3 cut(s) 393, 1123, 1843
BsmI GAATGC 1 cut(s) 2231
BsnI GGCC 3 cut(s) 502, 796, 2121
BsoBI CYCGRG 1 cut(s) 1180
Bsp119I TTCGAA 1 cut(s) 2025
Bsp1286I GDGCHC 5 cut(s) 599, 712, 1479, 2475, 2719
Bsp143I GATC 6 cut(s) 242, 304, 418, 426, 454, 1549
BspACI CCGC 8 cut(s) 377, 645, 1378, 1471, 1935, 2457, 2587, 2785
BspANI GGCC 3 cut(s) 502, 796, 2121
BspCNI CTCAG 5 cut(s) 534, 960, 1302, 1573, 2176
BspFNI CGCG 1 cut(s) 12
BspLI GGNNCC 5 cut(s) 649, 709, 1509, 1544, 1831
BspMAI CTGCAG 1 cut(s) 2646
BspPI GGATC 2 cut(s) 250, 299
BspT104I TTCGAA 1 cut(s) 2025
BspT107I GGYRCC 2 cut(s) 1507, 1542
BspTI CTTAAG 2 cut(s) 815, 1991
BsrBI CCGCTC 1 cut(s) 1935
BsrDI GCAATG 5 cut(s) 120, 411, 793, 847, 1814
BsrFI RCCGGY 1 cut(s) 716
BsrI ACTGG 7 cut(s) 40, 901, 962, 2163, 2216, 2599, 2743
BssAI RCCGGY 1 cut(s) 716
BssECI CCNNGG 5 cut(s) 640, 651, 1179, 1503, 2326
BssMI GATC 6 cut(s) 242, 304, 418, 426, 454, 1549
BssNI GRCGYC 1 cut(s) 2069
BssT1I CCWWGG 2 cut(s) 651, 2326
Bst2UI CCWGG 1 cut(s) 2240
Bst4CI ACNGT 6 cut(s) 414, 466, 1193, 1542, 1812, 2099
Bst6I CTCTTC 3 cut(s) 998, 2397, 2598
BstACI GRCGYC 1 cut(s) 2069
BstAFI CTTAAG 2 cut(s) 815, 1991
BstBAI YACGTR 1 cut(s) 680
BstBI TTCGAA 1 cut(s) 2025
BstC8I GCNNGC 8 cut(s) 14, 134, 375, 630, 1376, 1595, 2207, 2517
BstDEI CTNAG 7 cut(s) 542, 968, 1310, 1326, 1581, 2184, 2711
BstDSI CCRYGG 1 cut(s) 640
BstF5I GGATG 7 cut(s) 244, 520, 1083, 1879, 2047, 2322, 2747
BstFNI CGCG 1 cut(s) 12
BstKTI GATC 6 cut(s) 245, 307, 421, 429, 457, 1552
BstMAI GTCTC 4 cut(s) 1164, 1242, 1346, 1525
BstMBI GATC 6 cut(s) 242, 304, 418, 426, 454, 1549
BstMWI GCNNNNNNNGC 6 cut(s) 22, 1238, 1759, 1883, 1941, 1996
BstNI CCWGG 1 cut(s) 2240
BstNSI RCATGY 8 cut(s) 603, 782, 1593, 1597, 1705, 2248, 2400, 2616
BstSCI CCNGG 1 cut(s) 2238
BstSFI CTRYAG 1 cut(s) 2642
BstSLI GKGCMC 1 cut(s) 2475
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 8 cut(s) 28, 787, 844, 1228, 2214, 2380, 2628, 2656
BstV2I GAAGAC 4 cut(s) 270, 379, 1649, 1886
BstX2I RGATCY 2 cut(s) 242, 426
BstXI CCANNNNNNTGG 1 cut(s) 2365
BstYI RGATCY 2 cut(s) 242, 426
BsuI GTATCC 1 cut(s) 451
BsuRI GGCC 3 cut(s) 502, 796, 2121
BtgI CCRYGG 1 cut(s) 640
BtgZI GCGATG 1 cut(s) 192
BtsCI GGATG 7 cut(s) 244, 520, 1083, 1879, 2047, 2322, 2747
BtsIMutI CAGTG 1 cut(s) 2223
Cac8I GCNNGC 8 cut(s) 14, 134, 375, 630, 1376, 1595, 2207, 2517
Cfr10I RCCGGY 1 cut(s) 716
Cfr13I GGNCC 2 cut(s) 501, 1830
CseI GACGC 1 cut(s) 275
Csp6I GTAC 4 cut(s) 50, 965, 1543, 2782
CviQI GTAC 4 cut(s) 50, 965, 1543, 2782
DdeI CTNAG 7 cut(s) 542, 968, 1310, 1326, 1581, 2184, 2711
DpnI GATC 6 cut(s) 244, 306, 420, 428, 456, 1551
DpnII GATC 6 cut(s) 242, 304, 418, 426, 454, 1549
DraI TTTAAA 1 cut(s) 735
EaeI YGGCCR 1 cut(s) 2119
Eam1104I CTCTTC 3 cut(s) 998, 2397, 2598
EarI CTCTTC 3 cut(s) 998, 2397, 2598
EciI GGCGGA 1 cut(s) 660
Eco130I CCWWGG 2 cut(s) 651, 2326
Eco24I GRGCYC 1 cut(s) 712
Eco32I GATATC 1 cut(s) 1129
Eco47I GGWCC 1 cut(s) 1830
Eco57I CTGAAG 2 cut(s) 1383, 2129
Eco72I CACGTG 1 cut(s) 680
Eco88I CYCGRG 1 cut(s) 1180
EcoO109I RGGNCCY 1 cut(s) 1830
EcoRII CCWGG 1 cut(s) 2238
EcoRV GATATC 1 cut(s) 1129
EcoT14I CCWWGG 2 cut(s) 651, 2326
EcoT22I ATGCAT 4 cut(s) 1212, 1595, 2231, 2309
EcoT38I GRGCYC 1 cut(s) 712
ErhI CCWWGG 2 cut(s) 651, 2326
FalI AAGNNNNNCTT 2 cut(s) 995, 1027
FaqI GGGAC 3 cut(s) 393, 1123, 1843
FauI CCCGC 2 cut(s) 370, 1464
FauNDI CATATG 1 cut(s) 229
FblI GTMKAC 1 cut(s) 526
Fnu4HI GCNGC 9 cut(s) 17, 776, 858, 1242, 1936, 2203, 2394, 2642, 2645
FokI GGATG 7 cut(s) 251, 527, 1090, 1886, 2034, 2309, 2754
FriOI GRGCYC 1 cut(s) 712
Fsp4HI GCNGC 9 cut(s) 17, 776, 858, 1242, 1936, 2203, 2394, 2642, 2645
GluI GCNGC 9 cut(s) 17, 776, 858, 1242, 1936, 2203, 2394, 2642, 2645
GsaI CCCAGC 1 cut(s) 716
GsuI CTGGAG 2 cut(s) 613, 1266
HaeIII GGCC 3 cut(s) 502, 796, 2121
HapII CCGG 3 cut(s) 151, 717, 1404
HgaI GACGC 1 cut(s) 275
Hin1I GRCGYC 1 cut(s) 2069
HincII GTYRAC 4 cut(s) 527, 1587, 1743, 2610
HindII GTYRAC 4 cut(s) 527, 1587, 1743, 2610
HindIII AAGCTT 4 cut(s) 23, 1988, 2315, 2573
HpaII CCGG 3 cut(s) 151, 717, 1404
HphI GGTGA 7 cut(s) 20, 478, 682, 731, 987, 1145, 1235
Hpy166II GTNNAC 6 cut(s) 356, 527, 1587, 1743, 2473, 2610
Hpy188III TCNNGA 7 cut(s) 294, 424, 533, 592, 1094, 2705, 2765
Hpy8I GTNNAC 6 cut(s) 356, 527, 1587, 1743, 2473, 2610
HpyCH4III ACNGT 6 cut(s) 414, 466, 1193, 1542, 1812, 2099
HpyCH4IV ACGT 7 cut(s) 679, 883, 1571, 1613, 1681, 2069, 2234
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 1238, 1759, 1883, 1941, 1996
HpyF3I CTNAG 7 cut(s) 542, 968, 1310, 1326, 1581, 2184, 2711
HpySE526I ACGT 7 cut(s) 679, 883, 1571, 1613, 1681, 2069, 2234
Hsp92I GRCGYC 1 cut(s) 2069
KpnI GGTACC 1 cut(s) 1546
Kzo9I GATC 6 cut(s) 242, 304, 418, 426, 454, 1549
LmnI GCTCC 7 cut(s) 594, 637, 647, 707, 1150, 1932, 1996
Lsp1109I GCAGC 8 cut(s) 28, 787, 844, 1228, 2214, 2380, 2628, 2656
LweI GCATC 4 cut(s) 1799, 1828, 1864, 2732
MaeII ACGT 7 cut(s) 679, 883, 1571, 1613, 1681, 2069, 2234
MaeIII GTNAC 7 cut(s) 466, 490, 670, 680, 829, 1133, 1313
MalI GATC 6 cut(s) 244, 306, 420, 428, 456, 1551
MbiI CCGCTC 1 cut(s) 1935
MboI GATC 6 cut(s) 242, 304, 418, 426, 454, 1549
MfeI CAATTG 3 cut(s) 1457, 1922, 2418
MflI RGATCY 2 cut(s) 242, 426
MhlI GDGCHC 5 cut(s) 599, 712, 1479, 2475, 2719
MlsI TGGCCA 1 cut(s) 2121
MluNI TGGCCA 1 cut(s) 2121
MlyI GAGTC 7 cut(s) 351, 393, 733, 1420, 1592, 1738, 2678
MmeI TCCRAC 4 cut(s) 216, 1822, 1935, 2202
Mox20I TGGCCA 1 cut(s) 2121
Mph1103I ATGCAT 4 cut(s) 1212, 1595, 2231, 2309
MroXI GAANNNNTTC 1 cut(s) 2289
MscI TGGCCA 1 cut(s) 2121
MseI TTAA 9 cut(s) 84, 483, 734, 816, 1064, 1565, 1616, 1992, 2487
MslI CAYNNNNRTG 4 cut(s) 1824, 2439, 2478, 2617
Msp20I TGGCCA 1 cut(s) 2121
MspCI CTTAAG 2 cut(s) 815, 1991
MspI CCGG 3 cut(s) 151, 717, 1404
MspR9I CCNGG 1 cut(s) 2240
MunI CAATTG 3 cut(s) 1457, 1922, 2418
Mva1269I GAATGC 1 cut(s) 2231
MvaI CCWGG 1 cut(s) 2240
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 6 cut(s) 22, 1238, 1759, 1883, 1941, 1996
NdeI CATATG 1 cut(s) 229
NdeII GATC 6 cut(s) 242, 304, 418, 426, 454, 1549
NlaIV GGNNCC 5 cut(s) 649, 709, 1509, 1544, 1831
NmeAIII GCCGAG 2 cut(s) 339, 478
NmuCI GTSAC 4 cut(s) 466, 670, 680, 1133
NsiI ATGCAT 4 cut(s) 1212, 1595, 2231, 2309
NspI RCATGY 8 cut(s) 603, 782, 1593, 1597, 1705, 2248, 2400, 2616
NspV TTCGAA 1 cut(s) 2025
OliI CACNNNNGTG 1 cut(s) 2478
PaeI GCATGC 1 cut(s) 1597
PciI ACATGT 2 cut(s) 599, 2612
PctI GAATGC 1 cut(s) 2231
PdmI GAANNNNTTC 1 cut(s) 2289
PfeI GAWTC 3 cut(s) 611, 2103, 2369
PflMI CCANNNNNTGG 1 cut(s) 2771
PfoI TCCNGGA 1 cut(s) 2238
PkrI GCNGC 9 cut(s) 18, 777, 859, 1243, 1937, 2204, 2395, 2643, 2646
PleI GAGTC 7 cut(s) 351, 392, 733, 1420, 1591, 1738, 2678
PmaCI CACGTG 1 cut(s) 680
PmlI CACGTG 1 cut(s) 680
PpsI GAGTC 7 cut(s) 351, 392, 733, 1420, 1591, 1738, 2678
Ppu21I YACGTR 1 cut(s) 680
PpuMI RGGWCCY 1 cut(s) 1830
PscI ACATGT 2 cut(s) 599, 2612
PshBI ATTAAT 2 cut(s) 1064, 1565
PsiI TTATAA 1 cut(s) 2430
Psp5II RGGWCCY 1 cut(s) 1830
Psp6I CCWGG 1 cut(s) 2238
PspCI CACGTG 1 cut(s) 680
PspFI CCCAGC 1 cut(s) 712
PspGI CCWGG 1 cut(s) 2238
PspN4I GGNNCC 5 cut(s) 649, 709, 1509, 1544, 1831
PspPI GGNCC 2 cut(s) 501, 1830
PspPPI RGGWCCY 1 cut(s) 1830
PstI CTGCAG 1 cut(s) 2646
PsuI RGATCY 2 cut(s) 242, 426
RsaI GTAC 4 cut(s) 51, 966, 1544, 2783
RsaNI GTAC 4 cut(s) 50, 965, 1543, 2782
RseI CAYNNNNRTG 4 cut(s) 1824, 2439, 2478, 2617
SalI GTCGAC 1 cut(s) 525
SaqAI TTAA 9 cut(s) 84, 483, 734, 816, 1064, 1565, 1616, 1992, 2487
SatI GCNGC 9 cut(s) 17, 776, 858, 1242, 1936, 2203, 2394, 2642, 2645
Sau3AI GATC 6 cut(s) 242, 304, 418, 426, 454, 1549
Sau96I GGNCC 2 cut(s) 501, 1830
ScaI AGTACT 1 cut(s) 966
SchI GAGTC 7 cut(s) 351, 393, 733, 1420, 1592, 1738, 2678
ScrFI CCNGG 1 cut(s) 2240
SduI GDGCHC 5 cut(s) 599, 712, 1479, 2475, 2719
SfaNI GCATC 4 cut(s) 1799, 1828, 1864, 2732
SfcI CTRYAG 1 cut(s) 2642
SfuI TTCGAA 1 cut(s) 2025
SinI GGWCC 1 cut(s) 1830
SmiMI CAYNNNNRTG 4 cut(s) 1824, 2439, 2478, 2617
SmlI CTYRAG 7 cut(s) 20, 422, 815, 1094, 1834, 1991, 2720
SmoI CTYRAG 7 cut(s) 20, 422, 815, 1094, 1834, 1991, 2720
SphI GCATGC 1 cut(s) 1597
SsiI CCGC 8 cut(s) 377, 645, 1378, 1471, 1935, 2457, 2587, 2785
SspI AATATT 4 cut(s) 699, 1142, 1620, 2662
StyD4I CCNGG 1 cut(s) 2238
StyI CCWWGG 2 cut(s) 651, 2326
TaaI ACNGT 6 cut(s) 414, 466, 1193, 1542, 1812, 2099
TaiI ACGT 7 cut(s) 682, 886, 1574, 1616, 1684, 2072, 2237
TaqI TCGA 5 cut(s) 526, 2025, 2101, 2293, 2650
TaqII GACCGA 2 cut(s) 1152, 1517
TatI WGTACW 1 cut(s) 964
TauI GCSGC 1 cut(s) 1938
TfiI GAWTC 3 cut(s) 611, 2103, 2369
Tru1I TTAA 9 cut(s) 84, 483, 734, 816, 1064, 1565, 1616, 1992, 2487
Tru9I TTAA 9 cut(s) 84, 483, 734, 816, 1064, 1565, 1616, 1992, 2487
TscAI CASTG 1 cut(s) 2223
TseFI GTSAC 4 cut(s) 466, 670, 680, 1133
TseI GCWGC 8 cut(s) 16, 775, 857, 1241, 2202, 2393, 2641, 2644
Tsp45I GTSAC 4 cut(s) 466, 670, 680, 1133
TspGWI ACGGA 2 cut(s) 629, 1507
TspRI CASTG 1 cut(s) 2223
Van91I CCANNNNNTGG 1 cut(s) 2771
Vha464I CTTAAG 2 cut(s) 815, 1991
VneI GTGCAC 1 cut(s) 2471
VpaK11BI GGWCC 1 cut(s) 1830
VspI ATTAAT 2 cut(s) 1064, 1565
XapI RAATTY 8 cut(s) 86, 802, 1046, 1513, 2027, 2289, 2581, 2652
XceI RCATGY 8 cut(s) 603, 782, 1593, 1597, 1705, 2248, 2400, 2616
XcmI CCANNNNNNNNNTGG 1 cut(s) 346
XmiI GTMKAC 1 cut(s) 526
XmnI GAANNNNTTC 1 cut(s) 2289
ZraI GACGTC 1 cut(s) 2070
ZrmI AGTACT 1 cut(s) 966
Zsp2I ATGCAT 4 cut(s) 1212, 1595, 2231, 2309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.