Prupe.8G117900_v2.0.a1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000070
Physical Location & Seq
Forward (+)
14464981 .. 14477105
12125 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G117900.1

Sequence Viewer

Length: 4185 bp
ATGGTAGCAGTGGCAGAGGTCTTTCTTGGGGCACTCCTTCGGTTTCTGCTGGACAGGTTATTGACTCCACTCAACTTCCTCGCGGACGGTGTTGGCAAAAAACTGAAGACATGGAGTGAAATGTTGTCTTCAATTGAACTGGTGCTTCGTGATGCAGAGAAGAAGCAACTAACGAGCAGTGCAGTGGAGCTGTGGCTGGATGATCTCAAAGACTTGGCTTATGATATCGAAGACATCCTCGACAAGTTTTATACTGAGATGTTACGGCGCAAGTTGGACGAACAACGTGGGGCTACAACAAGTAAGGTACGAAGCCTAATTCCTAGATTTGAAGTCCACTTTAATATGAACTTCGAAATAAAGGAGATTACCAATCGGCTACAAGCCATATCTGAACGAAAAGATAAACTTGGGTTAAGTTTAGATGGTATTGGATCTTCCTCCACTAAACCTTGGCCAAGGCCACCAAGTTCATGTGTTCTGAATGGCGTGCCTGTTGTTGGAAGAGATAGTGACAAAGCAAAGATTGTTGAATTGTTGTCAAGAAACTATGAGCCTAATGCTATCAATTTTCAAGTAGTTGCGATTGTTGGCATGCCTGGAATTGGAAAAACAACACTGGCTCAATTTGTATTCAATGAGGATGATGTGCTGAAACAGTTTGATCTTAAGGCATGGGTATCCGTGTCCGATGAATTCGACGTTGTAAGTGTAACAAAGGCAATTCTTGAATCAGTCACGTCCGGACGCTGTGATTTGGAGGAATTCAGTAACATTCAGAACAATTTGAGCAAAGCATTGGCAGGAAAAAAGTTTCTGATTGTTTTAGATGATGTCTGGAATACATGTGACTATAATCTATGGACAATGCTGCAATCCCCCTTTTGTGTTGGAGCATCGGGAAGTAAGATAATCGTGACAACACGCGATGCAGAAGTTCCAAGAATGATGAGATCCACTGAAGTTCACAATTTGAGCGGTATATCAAATGGTGATTGCTGGAAAGTATTTATGCAACATGCATTTTTTAATATTGAAGAAAGCTCCAGACCAACGAAGTATGAATTACTTCAGGAGAAAATTGTTGCAAAATGTTGTGGGTTGCCTTTGGCGGCAAGGACTCTTGGTGGTCTTTTAGGTTGTAAAGAAATAAATGAATGGGAGGAAATATTGAACAACAAATTGTGGTTTCTATCTGATAAGAGTGGAATTCTCCCAGTATTGAAAATTAGCTACTATTATCTCCCTTCAACTTTGAAAAGGTGTTTTGCCTATTGCTCAATACTTCCAAATGACTATGAATTTGGGGAGACACAGTTGATCCTTCTATGGATGGCAGAAGATTTGATTCAGAAACCAGAAGAAAATAAACAATTGGAAGACGTAGGCCGTGAGTATTTTCAAGAGCTTGTGTCTAGGTCCTTATTTCAAAAATCAAGCAAACAAGATTCAAGATATGTGATGCATCACCTCATTAGTGATTTGGCACAAAAGGTTTCCGGAGAAACCTGTTTAAGATTGGAGGATATCTTGGATGGTAGATGGTCTCCGAAGACTCGTCATTTGTCTTACACTGCTGGTAAATATGATGGAGTCAAAAGATTTGAGGCGTTTGCTAAAGCAAAAGTTTTGCGGACTTTTCTACCACTTTCAATTTCACAGGATCCATGTAATTATTTGACTTGTAGGGTTACTTTTGAGTTACTGCCAAAGTTGCAATACATACGAGTGCTCTCTTTGAATGGTTATCGATTAATCAAGCTGCCGAATTCAATTGGTGAGTTGAAGTTTCTCCGATACCTAGATCTTTCTCACACGGAAATAACGAGTTTGCCTCGTTCAATAAGCACACTTTGCAACTTACAAACGTTGATATTAGAGAATTGTTATAGTTTGAAGGCACTACCTGCAAACATGAAGAACCTAATTAATCTGCGTCATCTCAACAATTCAAACACACCTTCATTGCAAGGAATGCCTGCACAACTAGGTCAACTGACGAATTTGAAAACATTGAGTAATTTTGTGGTGAGCGAAGGTAGGGAGTCGAGTATAAGAGAGGTAGAGCCCCTATTGCATCTCCAGGGGACATTGCGCCTCTCAAGATTGCAGAATGTGAATGACATTGAGGATGTGAAGAGGGCGGACTTAATTAGCAAGGCCGGGCTTGATGTATTGCTACTAGAATGGAATGGCTTAGGAGAAAAGGAATCCGACGTGCTCGACATGTTACAACCTCATAGGAAGCTCAAAGTGCTCAGCATCAAGGGATATGGTGGTTTGGAATTTTCAAAATGGATTGGACATCCTTTACTCTCTAGCTTGACAACGGTGTGCTTAGAGGGTTGCAACCATTGCTGTCTGTTGCCATCACTTGGACAATTGCCATCTCTCAAAAAGCTTTCTATAAAAAGATTGTGTGCAGTGGAAGTAGTGGGTCTTGAGTTCTATGGAACGCTAAGAATGCCTTTTCCACTGTTAGAGATCCTTGAGTTTGAGGATATGAAACATTGGAGGGAGTGGTTTCCATACGAACAAGATCAAGATCAAGGAATTAGAGTTTTCCCTTGCCTGAAAATGCTTTCCATATCCAAATGCCCCAAATTGGAGGGCAGGTTACCCGAGAACCTTGATTCACTGTCAAAGCTTGTGATTCGTGGATGTGAGCAGTTGGTGATTTCAATTTCTGACTATAAACAACTTCAGAAATTAGATATTGATGATTGCAAAAGGGTGGTCCATATAAAAGTTCAGCTTGAGTTATTGGAGGCCTTGCAGCTTTCAAGTATTGCAGAGTTCAAACTCCAAATAAAGGATTTCATGGGAGGATTACCAAAGCTTAATGATCTGGTTATTAGCGGTTGTGATGAGCTCACATCTTTATGGCAGAATGAAGATAAATTGCTCCATAACCTGATTTCTCTTCGTTGTTTGGTTATTGGAGACAACCCTCACCCCCATCTTGTTCAAAATCTTGTCTCACTCCAAGAGCTTCACATATATGACTGCCCAAGTCTGATTTCTTTTCAAGAGTTTCTTTTGCCACCTTTTGTCAAGGAAATAAAAATTGAGCGGTGCAGTTCTCTTGTGTATTTTGCAAGATACCGGATACCTCCAAATCTAAGAAGAATGGATATTATGTTATGCGAAAATTTGAAATCACTGCTGGAGGAAGAGGAGGTAAAGGGTTCTTCGTCATCCTCTTCTCCTTACCTGGTGAAAGAAGAGGAATCTTGTCTTGAGTACTTGAGTATAGAGGATTGTCCATTGCTGACGTCCTTATCATTTGAAGACCATCTTCCCGGGACGCTTAAACATCTTCGCATAAGCGATTGTGAACAGCTAGAGACAATAACCAATAGGTTCAAGCATAACACTTGCCTTGAAGAAATTAAGATCTCGCGTTGTAAAAATCTCAAGTGTTTACCGGAAGGCCTATGCTACCTCACCAATCTTCAAGAGTTAGGTATTTATGACTGTGCAAGTCTTGTTTCCTTCCCCGAAGGAGGGTTGCCACAAAGCGCTGCCTACCTAAGAGAGATCGACATCAGCTACTGCAACAAATTGGAGGCACTACCCAAAGGCATACATGACCTCAACTCTCTTCAGATATTGAGCATTTCTTGTTGCGAAGGTTTCACGCAGTTTCTAGAAGATGGTTTTCCCCCTAACTTAATACAGCTTACAATTTATAATCTCAAGAGCTGTAAGGCACTCTTGGACTTGGGGTTGCACAGGCTCACCTCTCTTAGAGAACTGGAAATTCGTGGCAAAGACCCAGACGTGTTGTTCTTCCCTCCTGAGAAAGAGATGGTGCTTCCTAAATCTCTCATTCGACTTACAATTCAGGACTTCCCGAATCTCGTTAAACTCAGCAACGGCTTTCAACTCCTCAACTGTCTTCAATCTTTTCACATCGAGGGGTGTCCTAAGCTAGCATCAATGGCAGAGGAGAGTCTTCCTCTATCACTTACACAACTTACTATCTATCACTGTCCGCTGCTAGAAGAGAGATGCAAACCAAGTAAAGGAAGATATTGGCCCTCCATAGCGCACATCCCTTACATTCGGATTCAAGACCACGTGCATAGAGCAAATCCAGATCATAGCCCGCGCCTCCCGTTCTCGTGCTCTCCTTCCAGATCAGAGGACCAGAGGACCAAGCTAGCTATCACGAACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1395

Amino Acids

158.46

Weight (kDa)

6.11

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3693
AatII GACGTC 1 cut(s) 3278
Acc36I ACCTGC 2 cut(s) 1915, 2604
AccB7I CCANNNNNTGG 1 cut(s) 2375
AccBSI CCGCTC 2 cut(s) 978, 3073
AccII CGCG 4 cut(s) 83, 927, 3403, 4114
AccIII TCCGGA 2 cut(s) 743, 1499
AciI CCGC 9 cut(s) 83, 978, 1112, 1633, 2144, 2859, 3073, 3998, 4112
AclI AACGTT 1 cut(s) 1868
AclWI GGATC 6 cut(s) 442, 948, 1315, 1658, 1671, 2479
AcoI YGGCCR 1 cut(s) 455
AcsI RAATTY 9 cut(s) 695, 764, 1209, 1301, 1768, 2002, 2285, 3151, 3762
AcuI CTGAAG 5 cut(s) 125, 981, 1055, 2687, 3590
AcvI CACGTG 1 cut(s) 4084
AcyI GRCGYC 1 cut(s) 3275
AfaI GTAC 2 cut(s) 309, 3245
AfeI AGCGCT 1 cut(s) 3523
AfiI CCNNNNNNNGG 8 cut(s) 500, 605, 2375, 2605, 2812, 3506, 3507, 4028
AflII CTTAAG 1 cut(s) 668
AflIII ACRYGT 3 cut(s) 845, 2226, 3783
AjiI CACGTC 3 cut(s) 741, 2218, 3784
AjnI CCWGG 3 cut(s) 598, 2082, 3213
AjuI GAANNNNNNNTTGG 2 cut(s) 3004, 3036
Alw21I GWGCWC 5 cut(s) 1734, 2223, 2259, 2874, 4133
Alw26I GTCTC 5 cut(s) 1304, 1551, 2937, 2983, 3341
AlwI GGATC 6 cut(s) 442, 948, 1315, 1658, 1671, 2479
AlwNI CAGNNNCTG 1 cut(s) 3555
Ama87I CYCGRG 2 cut(s) 2621, 3302
Aor13HI TCCGGA 2 cut(s) 743, 1499
Aor51HI AGCGCT 1 cut(s) 3523
AoxI GGCC 7 cut(s) 455, 461, 1387, 2160, 2769, 3433, 4040
ApeKI GCWGC 5 cut(s) 871, 1762, 2776, 3524, 4000
ApoI RAATTY 9 cut(s) 695, 764, 1209, 1301, 1768, 2002, 2285, 3151, 3762
ArsI GACNNNNNNTTYG 6 cut(s) 1286, 1318, 1546, 1578, 2721, 2753
AseI ATTAAT 2 cut(s) 1754, 1929
Asp700I GAANNNNTTC 1 cut(s) 1068
AspLEI GCGC 5 cut(s) 270, 2097, 3524, 4054, 4116
AspS9I GGNCC 5 cut(s) 1419, 2737, 4041, 4150, 4158
AsuC2I CCSGG 3 cut(s) 2164, 3303, 3304
AsuHPI GGTGA 9 cut(s) 1004, 1460, 1790, 2041, 2686, 2945, 3229, 3439, 3733
AsuII TTCGAA 1 cut(s) 354
AsuNHI GCTAGC 2 cut(s) 3934, 4165
AvaI CYCGRG 2 cut(s) 2621, 3302
AvaII GGWCC 4 cut(s) 1419, 2737, 4150, 4158
BaeGI GKGCMC 1 cut(s) 34
BalI TGGCCA 1 cut(s) 457
BamHI GGATCC 1 cut(s) 1663
BanII GRGCYC 2 cut(s) 2070, 2874
BarI GAAGNNNNNNTAC 2 cut(s) 421, 453
BauI CACGAG 1 cut(s) 4126
BbrPI CACGTG 1 cut(s) 4084
BbsI GAAGAC 8 cut(s) 113, 120, 237, 1386, 1559, 3297, 3893, 3950
Bbv12I GWGCWC 5 cut(s) 1734, 2223, 2259, 2874, 4133
BbvI GCAGC 5 cut(s) 858, 1749, 2788, 3511, 3987
BceAI ACGGC 3 cut(s) 281, 1374, 3895
BciT130I CCWGG 3 cut(s) 600, 2084, 3215
BciVI GTATCC 2 cut(s) 691, 3102
BcnI CCSGG 3 cut(s) 2164, 3303, 3304
BcoDI GTCTC 5 cut(s) 1304, 1551, 2937, 2983, 3341
BfoI RGCGCY 1 cut(s) 3525
BfrI CTTAAG 1 cut(s) 668
BfuAI ACCTGC 2 cut(s) 1915, 2604
BfuI GTATCC 2 cut(s) 691, 3102
BglII AGATCT 2 cut(s) 1804, 3396
BisI GCNGC 6 cut(s) 872, 1113, 1763, 2777, 3525, 4001
BlpI GCTNAGC 1 cut(s) 2258
BlsI GCNGC 6 cut(s) 873, 1114, 1764, 2778, 3526, 4002
BmcAI AGTACT 1 cut(s) 3245
Bme1390I CCNGG 6 cut(s) 600, 2084, 2164, 3215, 3303, 3304
Bme18I GGWCC 4 cut(s) 1419, 2737, 4150, 4158
BmeT110I CYCGRG 2 cut(s) 2621, 3302
BmgBI CACGTC 3 cut(s) 741, 2218, 3784
BmgT120I GGNCC 5 cut(s) 1419, 2737, 4041, 4150, 4158
BmiI GGNNCC 1 cut(s) 1665
BmrFI CCNGG 6 cut(s) 600, 2084, 2164, 3215, 3303, 3304
BmrI ACTGGG 1 cut(s) 1211
BmsI GCATC 9 cut(s) 142, 905, 919, 1452, 1474, 2086, 2271, 3947, 4004
BmtI GCTAGC 2 cut(s) 3938, 4169
BmuI ACTGGG 1 cut(s) 1211
BpiI GAAGAC 8 cut(s) 113, 120, 237, 1386, 1559, 3297, 3893, 3950
BplI GAGNNNNNCTC 4 cut(s) 1819, 1851, 3946, 3978
BpmI CTGGAG 3 cut(s) 1030, 2066, 3188
Bpu10I CCTNAGC 2 cut(s) 2197, 3930
Bpu1102I GCTNAGC 1 cut(s) 2258
Bpu14I TTCGAA 1 cut(s) 354
BpuEI CTTGAG 8 cut(s) 2086, 2462, 2510, 2777, 3260, 3268, 3401, 3683
BpuMI CCSGG 3 cut(s) 2164, 3303, 3304
Bsa29I ATCGAT 1 cut(s) 1750
BsaAI YACGTR 1 cut(s) 4084
BsaBI GATNNNNATC 2 cut(s) 2544, 3545
BsaHI GRCGYC 1 cut(s) 3275
BsaI GGTCTC 1 cut(s) 1551
BsaJI CCNNGG 4 cut(s) 452, 458, 2083, 3302
BsaWI WCCGGW 4 cut(s) 743, 1499, 3105, 3427
BsaXI ACNNNNNCTCC 4 cut(s) 1301, 1331, 2600, 2630
Bsc4I CCNNNNNNNGG 8 cut(s) 500, 605, 2375, 2605, 2812, 3506, 3507, 4028
Bse1I ACTGG 4 cut(s) 144, 624, 1217, 3762
Bse3DI GCAATG 4 cut(s) 1964, 2090, 2353, 3266
Bse8I GATNNNNATC 2 cut(s) 2544, 3545
BseAI TCCGGA 2 cut(s) 743, 1499
BseBI CCWGG 3 cut(s) 600, 2084, 3215
BseCI ATCGAT 1 cut(s) 1750
BseDI CCNNGG 4 cut(s) 452, 458, 2083, 3302
BseJI GATNNNNATC 2 cut(s) 2544, 3545
BseLI CCNNNNNNNGG 8 cut(s) 500, 605, 2375, 2605, 2812, 3506, 3507, 4028
BseMI GCAATG 4 cut(s) 1964, 2090, 2353, 3266
BseMII CTCAG 4 cut(s) 246, 2272, 3792, 3886
BseNI ACTGG 4 cut(s) 144, 624, 1217, 3762
BseRI GAGGAG 3 cut(s) 3191, 3881, 3965
BseSI GKGCMC 1 cut(s) 34
BseXI GCAGC 5 cut(s) 858, 1749, 2788, 3511, 3987
BsgI GTGCAG 4 cut(s) 201, 1965, 2442, 3097
Bsh1236I CGCG 4 cut(s) 83, 927, 3403, 4114
BshFI GGCC 7 cut(s) 457, 463, 1389, 2162, 2771, 3435, 4042
BshVI ATCGAT 1 cut(s) 1750
BsiHKAI GWGCWC 5 cut(s) 1734, 2223, 2259, 2874, 4133
BsiHKCI CYCGRG 2 cut(s) 2621, 3302
BsiSI CCGG 6 cut(s) 744, 1500, 2163, 3106, 3303, 3428
BslFI GGGAC 2 cut(s) 2101, 3319
BslI CCNNNNNNNGG 8 cut(s) 500, 605, 2375, 2605, 2812, 3506, 3507, 4028
BsmAI GTCTC 5 cut(s) 1304, 1551, 2937, 2983, 3341
BsmFI GGGAC 2 cut(s) 2101, 3319
BsmI GAATGC 2 cut(s) 1980, 2469
BsnI GGCC 7 cut(s) 457, 463, 1389, 2162, 2771, 3435, 4042
Bso31I GGTCTC 1 cut(s) 1551
BsoBI CYCGRG 2 cut(s) 2621, 3302
Bsp119I TTCGAA 1 cut(s) 354
Bsp1286I GDGCHC 7 cut(s) 34, 1734, 2070, 2223, 2259, 2874, 4133
Bsp13I TCCGGA 2 cut(s) 743, 1499
Bsp1720I GCTNAGC 1 cut(s) 2258
BspACI CCGC 9 cut(s) 83, 978, 1112, 1633, 2144, 2859, 3073, 3998, 4112
BspANI GGCC 7 cut(s) 457, 463, 1389, 2162, 2771, 3435, 4042
BspCNI CTCAG 4 cut(s) 247, 2271, 3793, 3885
BspDI ATCGAT 1 cut(s) 1750
BspEI TCCGGA 2 cut(s) 743, 1499
BspFNI CGCG 4 cut(s) 83, 927, 3403, 4114
BspLI GGNNCC 1 cut(s) 1665
BspMI ACCTGC 2 cut(s) 1915, 2604
BspOI GCTAGC 2 cut(s) 3938, 4169
BspPI GGATC 6 cut(s) 442, 948, 1315, 1658, 1671, 2479
BspT104I TTCGAA 1 cut(s) 354
BspTI CTTAAG 1 cut(s) 668
BspTNI GGTCTC 1 cut(s) 1551
BsrBI CCGCTC 2 cut(s) 978, 3073
BsrDI GCAATG 4 cut(s) 1964, 2090, 2353, 3266
BsrI ACTGG 4 cut(s) 144, 624, 1217, 3762
BssECI CCNNGG 4 cut(s) 452, 458, 2083, 3302
BssNI GRCGYC 1 cut(s) 3275
BssSI CACGAG 1 cut(s) 4126
BssT1I CCWWGG 2 cut(s) 452, 458
Bst2BI CACGAG 1 cut(s) 4126
Bst2UI CCWGG 3 cut(s) 600, 2084, 3215
Bst4CI ACNGT 9 cut(s) 89, 660, 1317, 2332, 2478, 2640, 3479, 3899, 3995
Bst6I CTCTTC 8 cut(s) 499, 2132, 2928, 3168, 3208, 3219, 3609, 4002
BstACI GRCGYC 1 cut(s) 3275
BstAFI CTTAAG 1 cut(s) 668
BstAPI GCANNNNNTGC 4 cut(s) 1854, 1907, 1975, 2355
BstBAI YACGTR 1 cut(s) 4084
BstBI TTCGAA 1 cut(s) 354
BstC8I GCNNGC 6 cut(s) 491, 596, 1980, 3936, 4112, 4167
BstEII GGTNACC 1 cut(s) 2616
BstFNI CGCG 4 cut(s) 83, 927, 3403, 4114
BstH2I RGCGCY 1 cut(s) 3525
BstHHI GCGC 5 cut(s) 270, 2097, 3524, 4054, 4116
BstMAI GTCTC 5 cut(s) 1304, 1551, 2937, 2983, 3341
BstMWI GCNNNNNNNGC 9 cut(s) 1714, 1854, 1907, 1975, 2074, 2254, 2355, 2464, 3944
BstNI CCWGG 3 cut(s) 600, 2084, 3215
BstNSI RCATGY 4 cut(s) 598, 849, 1022, 2230
BstPI GGTNACC 1 cut(s) 2616
BstSCI CCNGG 6 cut(s) 598, 2082, 2162, 3213, 3301, 3302
BstSLI GKGCMC 1 cut(s) 34
BstUI CGCG 4 cut(s) 83, 927, 3403, 4114
BstV1I GCAGC 5 cut(s) 858, 1749, 2788, 3511, 3987
BstV2I GAAGAC 8 cut(s) 113, 120, 237, 1386, 1559, 3297, 3893, 3950
BstX2I RGATCY 6 cut(s) 434, 953, 1663, 1804, 2484, 3396
BstYI RGATCY 6 cut(s) 434, 953, 1663, 1804, 2484, 3396
Bsu15I ATCGAT 1 cut(s) 1750
BsuI GTATCC 2 cut(s) 691, 3102
BsuRI GGCC 7 cut(s) 457, 463, 1389, 2162, 2771, 3435, 4042
BsuTUI ATCGAT 1 cut(s) 1750
BtgZI GCGATG 1 cut(s) 942
BtrI CACGTC 3 cut(s) 741, 2218, 3784
BtsI GCAGTG 6 cut(s) 15, 184, 189, 1572, 2430, 3161
BveI ACCTGC 2 cut(s) 1915, 2604
Cac8I GCNNGC 6 cut(s) 491, 596, 1980, 3936, 4112, 4167
CaiI CAGNNNCTG 1 cut(s) 3555
CfoI GCGC 5 cut(s) 270, 2097, 3524, 4054, 4116
Cfr13I GGNCC 5 cut(s) 1419, 2737, 4041, 4150, 4158
Cfr9I CCCGGG 1 cut(s) 3302
ClaI ATCGAT 1 cut(s) 1750
CseI GACGC 3 cut(s) 756, 1925, 3316
CsiI ACCWGGT 1 cut(s) 3213
Csp6I GTAC 2 cut(s) 308, 3244
CviQI GTAC 2 cut(s) 308, 3244
EaeI YGGCCR 1 cut(s) 455
Eam1104I CTCTTC 8 cut(s) 499, 2132, 2928, 3168, 3208, 3219, 3609, 4002
EarI CTCTTC 8 cut(s) 499, 2132, 2928, 3168, 3208, 3219, 3609, 4002
EciI GGCGGA 1 cut(s) 2159
Ecl136II GAGCTC 1 cut(s) 2872
Eco130I CCWWGG 2 cut(s) 452, 458
Eco147I AGGCCT 2 cut(s) 2771, 3435
Eco24I GRGCYC 2 cut(s) 2070, 2874
Eco31I GGTCTC 1 cut(s) 1551
Eco32I GATATC 2 cut(s) 226, 1528
Eco47I GGWCC 4 cut(s) 1419, 2737, 4150, 4158
Eco47III AGCGCT 1 cut(s) 3523
Eco53kI GAGCTC 1 cut(s) 2872
Eco57I CTGAAG 5 cut(s) 125, 981, 1055, 2687, 3590
Eco72I CACGTG 1 cut(s) 4084
Eco88I CYCGRG 2 cut(s) 2621, 3302
Eco91I GGTNACC 1 cut(s) 2616
EcoICRI GAGCTC 1 cut(s) 2872
EcoO109I RGGNCCY 1 cut(s) 1419
EcoO65I GGTNACC 1 cut(s) 2616
EcoRI GAATTC 4 cut(s) 695, 764, 1209, 1768
EcoRII CCWGG 3 cut(s) 598, 2082, 3213
EcoRV GATATC 2 cut(s) 226, 1528
EcoT14I CCWWGG 2 cut(s) 452, 458
EcoT22I ATGCAT 2 cut(s) 1024, 1467
EcoT38I GRGCYC 2 cut(s) 2070, 2874
ErhI CCWWGG 2 cut(s) 452, 458
FaqI GGGAC 2 cut(s) 2101, 3319
FauI CCCGC 1 cut(s) 4119
Fnu4HI GCNGC 6 cut(s) 872, 1113, 1763, 2777, 3525, 4001
FriOI GRGCYC 2 cut(s) 2070, 2874
Fsp4HI GCNGC 6 cut(s) 872, 1113, 1763, 2777, 3525, 4001
GlaI GCGC 5 cut(s) 269, 2096, 3523, 4053, 4115
GluI GCNGC 6 cut(s) 872, 1113, 1763, 2777, 3525, 4001
GsuI CTGGAG 3 cut(s) 1030, 2066, 3188
HaeII RGCGCY 1 cut(s) 3525
HaeIII GGCC 7 cut(s) 457, 463, 1389, 2162, 2771, 3435, 4042
HapII CCGG 6 cut(s) 744, 1500, 2163, 3106, 3303, 3428
HgaI GACGC 3 cut(s) 756, 1925, 3316
HhaI GCGC 5 cut(s) 270, 2097, 3524, 4054, 4116
Hin1I GRCGYC 1 cut(s) 3275
Hin6I GCGC 5 cut(s) 268, 2095, 3522, 4052, 4114
HinP1I GCGC 5 cut(s) 268, 2095, 3522, 4052, 4114
HincII GTYRAC 1 cut(s) 1994
HindII GTYRAC 1 cut(s) 1994
HindIII AAGCTT 3 cut(s) 2399, 2645, 2837
HpaII CCGG 6 cut(s) 744, 1500, 2163, 3106, 3303, 3428
HphI GGTGA 9 cut(s) 1004, 1460, 1790, 2041, 2686, 2945, 3229, 3439, 3733
Hpy166II GTNNAC 5 cut(s) 337, 967, 1994, 3338, 3425
Hpy8I GTNNAC 5 cut(s) 337, 967, 1994, 3338, 3425
Hpy99I CGWCG 2 cut(s) 704, 2219
HpyCH4III ACNGT 9 cut(s) 89, 660, 1317, 2332, 2478, 2640, 3479, 3899, 3995
HpyCH4IV ACGT 9 cut(s) 286, 702, 740, 1383, 1868, 2217, 3275, 3783, 4083
HpyF10VI GCNNNNNNNGC 9 cut(s) 1714, 1854, 1907, 1975, 2074, 2254, 2355, 2464, 3944
HpySE526I ACGT 9 cut(s) 286, 702, 740, 1383, 1868, 2217, 3275, 3783, 4083
Hsp92I GRCGYC 1 cut(s) 3275
HspAI GCGC 5 cut(s) 268, 2095, 3522, 4052, 4114
Kpn2I TCCGGA 2 cut(s) 743, 1499
LmnI GCTCC 4 cut(s) 187, 893, 1049, 2910
Lsp1109I GCAGC 5 cut(s) 858, 1749, 2788, 3511, 3987
LweI GCATC 9 cut(s) 142, 905, 919, 1452, 1474, 2086, 2271, 3947, 4004
MabI ACCWGGT 1 cut(s) 3213
MaeII ACGT 9 cut(s) 286, 702, 740, 1383, 1868, 2217, 3275, 3783, 4083
MbiI CCGCTC 2 cut(s) 978, 3073
MfeI CAATTG 4 cut(s) 132, 1373, 1773, 2381
MflI RGATCY 6 cut(s) 434, 953, 1663, 1804, 2484, 3396
MhlI GDGCHC 7 cut(s) 34, 1734, 2070, 2223, 2259, 2874, 4133
MlsI TGGCCA 1 cut(s) 457
MluNI TGGCCA 1 cut(s) 457
MlyI GAGTC 6 cut(s) 58, 1114, 1549, 1602, 2054, 3964
MmeI TCCRAC 4 cut(s) 255, 481, 871, 2238
Mox20I TGGCCA 1 cut(s) 457
Mph1103I ATGCAT 2 cut(s) 1024, 1467
MroI TCCGGA 2 cut(s) 743, 1499
MroXI GAANNNNTTC 1 cut(s) 1068
MscI TGGCCA 1 cut(s) 457
MslI CAYNNNNRTG 3 cut(s) 1727, 2881, 3000
Msp20I TGGCCA 1 cut(s) 457
MspA1I CMGCKG 1 cut(s) 4000
MspCI CTTAAG 1 cut(s) 668
MspI CCGG 6 cut(s) 744, 1500, 2163, 3106, 3303, 3428
MspR9I CCNGG 6 cut(s) 600, 2084, 2164, 3215, 3303, 3304
MunI CAATTG 4 cut(s) 132, 1373, 1773, 2381
Mva1269I GAATGC 2 cut(s) 1980, 2469
MvaI CCWGG 3 cut(s) 600, 2084, 3215
MvnI CGCG 4 cut(s) 83, 927, 3403, 4114
MwoI GCNNNNNNNGC 9 cut(s) 1714, 1854, 1907, 1975, 2074, 2254, 2355, 2464, 3944
NciI CCSGG 3 cut(s) 2164, 3303, 3304
NheI GCTAGC 2 cut(s) 3934, 4165
NlaIV GGNNCC 1 cut(s) 1665
NmuCI GTSAC 4 cut(s) 512, 736, 848, 916
NsiI ATGCAT 2 cut(s) 1024, 1467
NspI RCATGY 4 cut(s) 598, 849, 1022, 2230
NspV TTCGAA 1 cut(s) 354
PaeI GCATGC 1 cut(s) 598
PceI AGGCCT 2 cut(s) 2771, 3435
PciI ACATGT 2 cut(s) 845, 2226
PcsI WCGNNNNNNNCGW 1 cut(s) 1823
PctI GAATGC 2 cut(s) 1980, 2469
PdmI GAANNNNTTC 1 cut(s) 1068
PfeI GAWTC 9 cut(s) 731, 1348, 1448, 2210, 2633, 2653, 3230, 3859, 4072
PflMI CCANNNNNTGG 1 cut(s) 2375
PkrI GCNGC 6 cut(s) 873, 1114, 1764, 2778, 3526, 4002
PleI GAGTC 6 cut(s) 58, 1114, 1549, 1601, 2053, 3963
PmaCI CACGTG 1 cut(s) 4084
PmlI CACGTG 1 cut(s) 4084
PpsI GAGTC 6 cut(s) 58, 1114, 1549, 1601, 2053, 3963
Ppu21I YACGTR 1 cut(s) 4084
PpuMI RGGWCCY 1 cut(s) 1419
PscI ACATGT 2 cut(s) 845, 2226
PshBI ATTAAT 2 cut(s) 1754, 1929
PsiI TTATAA 1 cut(s) 3693
Psp124BI GAGCTC 1 cut(s) 2874
Psp1406I AACGTT 1 cut(s) 1868
Psp5II RGGWCCY 1 cut(s) 1419
Psp6I CCWGG 3 cut(s) 598, 2082, 3213
PspCI CACGTG 1 cut(s) 4084
PspEI GGTNACC 1 cut(s) 2616
PspGI CCWGG 3 cut(s) 598, 2082, 3213
PspN4I GGNNCC 1 cut(s) 1665
PspPI GGNCC 5 cut(s) 1419, 2737, 4041, 4150, 4158
PspPPI RGGWCCY 1 cut(s) 1419
PstNI CAGNNNCTG 1 cut(s) 3555
PsuI RGATCY 6 cut(s) 434, 953, 1663, 1804, 2484, 3396
RsaI GTAC 2 cut(s) 309, 3245
RsaNI GTAC 2 cut(s) 308, 3244
RseI CAYNNNNRTG 3 cut(s) 1727, 2881, 3000
SacI GAGCTC 1 cut(s) 2874
SatI GCNGC 6 cut(s) 872, 1113, 1763, 2777, 3525, 4001
Sau96I GGNCC 5 cut(s) 1419, 2737, 4041, 4150, 4158
ScaI AGTACT 1 cut(s) 3245
SchI GAGTC 6 cut(s) 58, 1114, 1549, 1602, 2054, 3964
ScrFI CCNGG 6 cut(s) 600, 2084, 2164, 3215, 3303, 3304
SduI GDGCHC 7 cut(s) 34, 1734, 2070, 2223, 2259, 2874, 4133
SexAI ACCWGGT 1 cut(s) 3213
SfaNI GCATC 9 cut(s) 142, 905, 919, 1452, 1474, 2086, 2271, 3947, 4004
SfuI TTCGAA 1 cut(s) 354
SinI GGWCC 4 cut(s) 1419, 2737, 4150, 4158
SmaI CCCGGG 1 cut(s) 3304
SmiMI CAYNNNNRTG 3 cut(s) 1727, 2881, 3000
SmlI CTYRAG 9 cut(s) 668, 2101, 2441, 2489, 2756, 3239, 3247, 3416, 3698
SmoI CTYRAG 9 cut(s) 668, 2101, 2441, 2489, 2756, 3239, 3247, 3416, 3698
SphI GCATGC 1 cut(s) 598
SseBI AGGCCT 2 cut(s) 2771, 3435
SsiI CCGC 9 cut(s) 83, 978, 1112, 1633, 2144, 2859, 3073, 3998, 4112
SspI AATATT 2 cut(s) 1033, 1170
SstI GAGCTC 1 cut(s) 2874
StuI AGGCCT 2 cut(s) 2771, 3435
StyD4I CCNGG 6 cut(s) 598, 2082, 2162, 3213, 3301, 3302
StyI CCWWGG 2 cut(s) 452, 458
TaaI ACNGT 9 cut(s) 89, 660, 1317, 2332, 2478, 2640, 3479, 3899, 3995
TaiI ACGT 9 cut(s) 289, 705, 743, 1386, 1871, 2220, 3278, 3786, 4086
TatI WGTACW 1 cut(s) 3243
TauI GCSGC 1 cut(s) 1115
TfiI GAWTC 9 cut(s) 731, 1348, 1448, 2210, 2633, 2653, 3230, 3859, 4072
TseFI GTSAC 4 cut(s) 512, 736, 848, 916
TseI GCWGC 5 cut(s) 871, 1762, 2776, 3524, 4000
Tsp45I GTSAC 4 cut(s) 512, 736, 848, 916
TspGWI ACGGA 2 cut(s) 673, 1832
TspMI CCCGGG 1 cut(s) 3302
Van91I CCANNNNNTGG 1 cut(s) 2375
Vha464I CTTAAG 1 cut(s) 668
VpaK11BI GGWCC 4 cut(s) 1419, 2737, 4150, 4158
VspI ATTAAT 2 cut(s) 1754, 1929
XapI RAATTY 9 cut(s) 695, 764, 1209, 1301, 1768, 2002, 2285, 3151, 3762
XbaI TCTAGA 1 cut(s) 3649
XceI RCATGY 4 cut(s) 598, 849, 1022, 2230
XmaI CCCGGG 1 cut(s) 3302
XmnI GAANNNNTTC 1 cut(s) 1068
ZraI GACGTC 1 cut(s) 3276
ZrmI AGTACT 1 cut(s) 3245
Zsp2I ATGCAT 2 cut(s) 1024, 1467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.