FvH4_7g25683

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000070
Physical Location & Seq
Forward (+)
19611891 .. 19616704
4814 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g25683.t1

Sequence Viewer

Length: 1995 bp
ATGGGGGAGGCTGTTCTTGGTGCATTCCTCCCGGTGCTGCTTGAGAAGTTGGCGGATCAAGAGATCACTGTGTACTTCGGACGCCTTAAGGGGGTCGACAAAAAGGTGCTGGAGAAATGGACTAGAACGTTGACGGCCATTGAAGCGGTGCTGAGTGATGCTGAGGAGAAGCAACTAACACAAAGAGCAGTAAAACAGTGGCTGGATGATCTCAGAGATTTGGCTTACGATGTTCAAGATGTATTGGACATATTTGATACTAAAATGCTGAAGCGGAGGATTGAAAAGAAACAAGGAAGCAAAAGCAAGCTATGGAGCTCACTCTCTATCTCTAAAGTTAAGTCCAATTTTGGTCTGAACTCAGCAATCAAGAAGATAAGTGATCGACTTGAGGAGATAAGTACTCGAGAGAAGCAGTTTGGTTTGAAGAAACTTGGAGTGTCTAAAAAGCCATGGAAAATGCCACCCACTACTTATCAACTTGACGGACCTGTGATCGGAAGGGATGAAGCAAAGGCTAAGATTATCCATGATTTGCTTTCAAAACAAGAAAATTATCATGTGGTTGGTATTGTCGGTACTGGAGGACTAGGAAAGACAACACTTGCTAAACATGTATTCAACGATGCTGCAACTGGCCAGTTTTCCCCAAAAGGATGGGTATCTGTGTCAGACGACTTTGACATTGTAAGGGTGGCAGCAGCCGTTTTGGAATCAGTCACGTCAGCTCCAGTTCAGGTCAAGGAGCTTAACTCAATTCTGGAGAAGATAAGTAAGGAATTGGCTGGCAAGAAGTTTTTAATTGTTCTTGATGATGTCTGGCGCACTTGTAGCTATGGTCAATGGACTACACTGCAGGCCTCCTTTCGTGTTGGAGCAGCTGGAAGTAAGATCATCGTGACAACACGTGACGCCAAAGTCGCAAAGATGATGGGAGATAGTAGCCCTTATAACTTGAAGCCGATGTCAGAAGATGATTGTTGGGAACTCTTTCAGCAACATGCACTCTTAAATAACAGACCTGAACATGTTGAGTTCCTTAAAGAGAAAATTGTAGAAAAATGCAATGGGTTACCGTTAGCTGCAAGGACTCTTGGTGGGCTTTTACGTTGTAAAGAAGTAGACGAATGGGAAGACATATTAGACCACAAGATGTGGACACTATCAGACTCAGACCAGAACAAGGAGGACGATATCCTTCCAGCATTAAAACTGAGCTATCATTATCTTCCTCCAACTTTGAAACGATGTTTCTCCTACTGTGCAATACTTCCAAATGATTATGAATTTGAGGAGATGCAATTGATCCTTTGGTGGATGGCAGAGGGTTTGATTCCGCAGCAACCCAAAGAACGTAAGCAAATGGAAGATTTGGGACGTGACTATTTTCATGAGTTGGTGTCTAGATCATTGTTTCAAAAATCAAGAAATGGAAAGTCGCTGTACATAATGCATGACCTGGTTACTGATTTGGCACGTTGGGCTGCAGGAAGTTCATGTTGTAGATTGGAGGATATGCAGAATTATGACTCGCATCAATGTACATGTTTGCCCAAGATTCGTCATTCGTCTTACATTCTTGGTATGTATGATGGAGTTAAGAAGTTTGAGGTCTATTCTGAAGCTACATCTTTGCGAACGTTCTTACCACTTGGGTCACTTTCAATTCATTATCTGGCTCATAAGGTTACTTCTTATTTATTGACCAAACTCCATTACTTACGACTGCTCTCTCTAAATGGATACCGAATAACCGAGTTGCCAGATACAATTGGTGAATTGAAGCATCTACGGTATCTTGATCTCTCCTACACATTGATAGAAAGTTTGCCAGACTCAACAACCACTCTTTACAACTTGCAGACATTGATATTGAAATGGTGTGGTCGGTTGAAGGCACTGCCCACAAGCATGAGGAACCTAGTTAATTTGCGTCATCTCAACAATTCAGTACACGGTCATTGGAAGAAATGCCTCCTCAGCTTGGTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

665

Amino Acids

75.7

Weight (kDa)

8.7

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 99 8e-21 Rx N-terminal domain
NB-ARC PF00931 172 - 336 9.3e-37 NB-ARC domain
WHD_DRP PF23559 423 - 492 1.5e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 564 - 650 1.4e-14 Leucine-rich repeat region
LRR_8 PF13855 574 - 627 8.1e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 951
AasI GACNNNNNNGTC 1 cut(s) 917
AccI GTMKAC 2 cut(s) 96, 1122
AciI CCGC 4 cut(s) 53, 146, 274, 1337
AclI AACGTT 2 cut(s) 128, 1640
AclWI GGATC 2 cut(s) 63, 1300
AcoI YGGCCR 2 cut(s) 135, 637
AcsI RAATTY 1 cut(s) 1286
AcuI CTGAAG 2 cut(s) 290, 1641
AcvI CACGTG 1 cut(s) 908
AcyI GRCGYC 2 cut(s) 82, 912
AfaI GTAC 6 cut(s) 74, 403, 580, 1445, 1543, 1953
AfiI CCNNNNNNNGG 4 cut(s) 91, 497, 1183, 1984
AflII CTTAAG 1 cut(s) 86
AflIII ACRYGT 4 cut(s) 613, 905, 1027, 1544
AjiI CACGTC 2 cut(s) 723, 1379
AjnI CCWGG 1 cut(s) 1458
Alw21I GWGCWC 1 cut(s) 320
AlwI GGATC 2 cut(s) 63, 1300
AlwNI CAGNNNCTG 1 cut(s) 202
Ama87I CYCGRG 1 cut(s) 405
AoxI GGCC 3 cut(s) 135, 637, 858
ApeKI GCWGC 8 cut(s) 37, 629, 698, 701, 878, 1082, 1339, 1484
ApoI RAATTY 1 cut(s) 1286
Asp700I GAANNNNTTC 1 cut(s) 990
AspLEI GCGC 1 cut(s) 825
AspS9I GGNCC 1 cut(s) 488
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 1 cut(s) 1787
AvaI CYCGRG 1 cut(s) 405
AvaII GGWCC 1 cut(s) 488
BalI TGGCCA 1 cut(s) 639
BanII GRGCYC 1 cut(s) 320
BbrPI CACGTG 1 cut(s) 908
BbsI GAAGAC 1 cut(s) 1140
Bbv12I GWGCWC 1 cut(s) 320
BbvCI CCTCAGC 2 cut(s) 162, 1979
BbvI GCAGC 8 cut(s) 24, 616, 710, 713, 890, 1069, 1351, 1471
BccI CCATC 4 cut(s) 651, 925, 1312, 1586
BceAI ACGGC 2 cut(s) 150, 689
BciT130I CCWGG 1 cut(s) 1460
BciVI GTATCC 1 cut(s) 1736
BcnI CCSGG 1 cut(s) 32
BfaI CTAG 4 cut(s) 123, 590, 1404, 1922
BfmI CTRYAG 2 cut(s) 854, 1485
BfrI CTTAAG 1 cut(s) 86
BfuI GTATCC 1 cut(s) 1736
BisI GCNGC 8 cut(s) 38, 630, 699, 702, 879, 1083, 1340, 1485
BlsI GCNGC 8 cut(s) 39, 631, 700, 703, 880, 1084, 1341, 1486
BmcAI AGTACT 1 cut(s) 403
Bme1390I CCNGG 2 cut(s) 32, 1460
Bme18I GGWCC 1 cut(s) 488
BmeT110I CYCGRG 1 cut(s) 405
BmgBI CACGTC 2 cut(s) 723, 1379
BmgT120I GGNCC 1 cut(s) 488
BmiI GGNNCC 1 cut(s) 1919
BmrFI CCNGG 2 cut(s) 32, 1460
BmsI GCATC 5 cut(s) 148, 616, 1287, 1543, 1795
BpiI GAAGAC 1 cut(s) 1140
BplI GAGNNNNNCTC 2 cut(s) 737, 769
BpmI CTGGAG 4 cut(s) 131, 603, 714, 782
Bpu10I CCTNAGC 2 cut(s) 162, 1979
BpuEI CTTGAG 2 cut(s) 62, 410
BpuMI CCSGG 1 cut(s) 32
BsaAI YACGTR 1 cut(s) 908
BsaBI GATNNNNATC 1 cut(s) 661
BsaHI GRCGYC 2 cut(s) 82, 912
BsaJI CCNNGG 1 cut(s) 452
BsaXI ACNNNNNCTCC 2 cut(s) 712, 742
Bsc4I CCNNNNNNNGG 4 cut(s) 91, 497, 1183, 1984
Bse1I ACTGG 4 cut(s) 586, 640, 640, 731
Bse3DI GCAATG 1 cut(s) 1072
Bse8I GATNNNNATC 1 cut(s) 661
BseBI CCWGG 1 cut(s) 1460
BseDI CCNNGG 1 cut(s) 452
BseGI GGATG 4 cut(s) 211, 511, 662, 1323
BseJI GATNNNNATC 1 cut(s) 661
BseLI CCNNNNNNNGG 4 cut(s) 91, 497, 1183, 1984
BseMI GCAATG 1 cut(s) 1072
BseMII CTCAG 7 cut(s) 143, 153, 226, 375, 1185, 1205, 1993
BseNI ACTGG 4 cut(s) 586, 640, 640, 731
BseRI GAGGAG 4 cut(s) 179, 407, 1307, 1967
BseXI GCAGC 8 cut(s) 24, 616, 710, 713, 890, 1069, 1351, 1471
BshFI GGCC 3 cut(s) 137, 639, 860
BsiHKAI GWGCWC 1 cut(s) 320
BsiHKCI CYCGRG 1 cut(s) 405
BsiSI CCGG 1 cut(s) 32
BslFI GGGAC 1 cut(s) 1389
BslI CCNNNNNNNGG 4 cut(s) 91, 497, 1183, 1984
BsmFI GGGAC 1 cut(s) 1389
BsmI GAATGC 1 cut(s) 23
BsnI GGCC 3 cut(s) 137, 639, 860
BsoBI CYCGRG 1 cut(s) 405
Bsp1286I GDGCHC 1 cut(s) 320
Bsp1407I TGTACA 2 cut(s) 1443, 1541
Bsp143I GATC 9 cut(s) 55, 63, 208, 382, 495, 891, 1305, 1406, 1801
Bsp19I CCATGG 1 cut(s) 452
BspACI CCGC 4 cut(s) 53, 146, 274, 1337
BspANI GGCC 3 cut(s) 137, 639, 860
BspCNI CTCAG 7 cut(s) 144, 154, 225, 374, 1184, 1206, 1992
BspHI TCATGA 1 cut(s) 1390
BspLI GGNNCC 1 cut(s) 1919
BspMAI CTGCAG 2 cut(s) 858, 1489
BspPI GGATC 2 cut(s) 63, 1300
BspTI CTTAAG 1 cut(s) 86
BsrDI GCAATG 1 cut(s) 1072
BsrGI TGTACA 2 cut(s) 1443, 1541
BsrI ACTGG 4 cut(s) 586, 640, 640, 731
BssECI CCNNGG 1 cut(s) 452
BssMI GATC 9 cut(s) 55, 63, 208, 382, 495, 891, 1305, 1406, 1801
BssNI GRCGYC 2 cut(s) 82, 912
BssT1I CCWWGG 1 cut(s) 452
Bst2UI CCWGG 1 cut(s) 1460
Bst4CI ACNGT 6 cut(s) 70, 198, 1077, 1262, 1794, 1958
BstACI GRCGYC 2 cut(s) 82, 912
BstAFI CTTAAG 1 cut(s) 86
BstAUI TGTACA 2 cut(s) 1443, 1541
BstBAI YACGTR 1 cut(s) 908
BstC8I GCNNGC 3 cut(s) 308, 787, 858
BstDEI CTNAG 8 cut(s) 152, 162, 212, 361, 519, 1171, 1214, 1979
BstDSI CCRYGG 1 cut(s) 452
BstEII GGTNACC 1 cut(s) 1071
BstF5I GGATG 4 cut(s) 211, 511, 662, 1323
BstHHI GCGC 1 cut(s) 825
BstKTI GATC 9 cut(s) 58, 66, 211, 385, 498, 894, 1308, 1409, 1804
BstMBI GATC 9 cut(s) 55, 63, 208, 382, 495, 891, 1305, 1406, 1801
BstMWI GCNNNNNNNGC 5 cut(s) 143, 831, 920, 1481, 1980
BstNI CCWGG 1 cut(s) 1460
BstNSI RCATGY 4 cut(s) 617, 1004, 1031, 1548
BstPI GGTNACC 1 cut(s) 1071
BstSCI CCNGG 2 cut(s) 30, 1458
BstSFI CTRYAG 2 cut(s) 854, 1485
BstV1I GCAGC 8 cut(s) 24, 616, 710, 713, 890, 1069, 1351, 1471
BstV2I GAAGAC 1 cut(s) 1140
BstXI CCANNNNNNTGG 1 cut(s) 657
BsuI GTATCC 1 cut(s) 1736
BsuRI GGCC 3 cut(s) 137, 639, 860
BtgI CCRYGG 1 cut(s) 452
BtrI CACGTC 2 cut(s) 723, 1379
BtsCI GGATG 4 cut(s) 211, 511, 662, 1323
BtsI GCAGTG 2 cut(s) 851, 1898
BtsIMutI CAGTG 4 cut(s) 66, 203, 851, 1898
Cac8I GCNNGC 3 cut(s) 308, 787, 858
CaiI CAGNNNCTG 1 cut(s) 202
CciI TCATGA 1 cut(s) 1390
CfoI GCGC 1 cut(s) 825
Cfr13I GGNCC 1 cut(s) 488
CseI GACGC 3 cut(s) 90, 920, 1922
CsiI ACCWGGT 1 cut(s) 1458
Csp6I GTAC 6 cut(s) 73, 402, 579, 1444, 1542, 1952
CviQI GTAC 6 cut(s) 73, 402, 579, 1444, 1542, 1952
DdeI CTNAG 8 cut(s) 152, 162, 212, 361, 519, 1171, 1214, 1979
DpnI GATC 9 cut(s) 57, 65, 210, 384, 497, 893, 1307, 1408, 1803
DpnII GATC 9 cut(s) 55, 63, 208, 382, 495, 891, 1305, 1406, 1801
DrdI GACNNNNNNGTC 1 cut(s) 917
DseDI GACNNNNNNGTC 1 cut(s) 917
EaeI YGGCCR 2 cut(s) 135, 637
EciI GGCGGA 1 cut(s) 68
Ecl136II GAGCTC 1 cut(s) 318
Eco130I CCWWGG 1 cut(s) 452
Eco147I AGGCCT 1 cut(s) 860
Eco24I GRGCYC 1 cut(s) 320
Eco32I GATATC 1 cut(s) 1195
Eco47I GGWCC 1 cut(s) 488
Eco53kI GAGCTC 1 cut(s) 318
Eco57I CTGAAG 2 cut(s) 290, 1641
Eco72I CACGTG 1 cut(s) 908
Eco88I CYCGRG 1 cut(s) 405
Eco91I GGTNACC 1 cut(s) 1071
EcoICRI GAGCTC 1 cut(s) 318
EcoO65I GGTNACC 1 cut(s) 1071
EcoRII CCWGG 1 cut(s) 1458
EcoRV GATATC 1 cut(s) 1195
EcoT14I CCWWGG 1 cut(s) 452
EcoT22I ATGCAT 1 cut(s) 1455
EcoT38I GRGCYC 1 cut(s) 320
ErhI CCWWGG 1 cut(s) 452
FaqI GGGAC 1 cut(s) 1389
FblI GTMKAC 2 cut(s) 96, 1122
Fnu4HI GCNGC 8 cut(s) 38, 630, 699, 702, 879, 1083, 1340, 1485
FokI GGATG 4 cut(s) 218, 518, 669, 1330
FriOI GRGCYC 1 cut(s) 320
Fsp4HI GCNGC 8 cut(s) 38, 630, 699, 702, 879, 1083, 1340, 1485
FspBI CTAG 4 cut(s) 123, 590, 1404, 1922
GlaI GCGC 1 cut(s) 824
GluI GCNGC 8 cut(s) 38, 630, 699, 702, 879, 1083, 1340, 1485
GsuI CTGGAG 4 cut(s) 131, 603, 714, 782
HaeIII GGCC 3 cut(s) 137, 639, 860
HapII CCGG 1 cut(s) 32
HgaI GACGC 3 cut(s) 90, 920, 1922
HhaI GCGC 1 cut(s) 825
Hin1I GRCGYC 2 cut(s) 82, 912
Hin6I GCGC 1 cut(s) 823
HinP1I GCGC 1 cut(s) 823
HincII GTYRAC 2 cut(s) 97, 132
HindII GTYRAC 2 cut(s) 97, 132
HinfI GANTC 7 cut(s) 713, 1090, 1169, 1333, 1529, 1558, 1835
HpaII CCGG 1 cut(s) 32
HphI GGTGA 1 cut(s) 1787
Hpy166II GTNNAC 6 cut(s) 73, 97, 132, 1123, 1158, 1954
Hpy188I TCNGA 9 cut(s) 80, 215, 357, 500, 673, 970, 1168, 1174, 1621
Hpy8I GTNNAC 6 cut(s) 73, 97, 132, 1123, 1158, 1954
HpyAV CCTTC 3 cut(s) 495, 1208, 1888
HpyCH4III ACNGT 6 cut(s) 70, 198, 1077, 1262, 1794, 1958
HpyCH4IV ACGT 8 cut(s) 128, 722, 907, 1108, 1354, 1378, 1477, 1640
HpyF10VI GCNNNNNNNGC 5 cut(s) 143, 831, 920, 1481, 1980
HpyF3I CTNAG 8 cut(s) 152, 162, 212, 361, 519, 1171, 1214, 1979
HpySE526I ACGT 8 cut(s) 128, 722, 907, 1108, 1354, 1378, 1477, 1640
Hsp92I GRCGYC 2 cut(s) 82, 912
HspAI GCGC 1 cut(s) 823
Kzo9I GATC 9 cut(s) 55, 63, 208, 382, 495, 891, 1305, 1406, 1801
LmnI GCTCC 4 cut(s) 315, 733, 745, 875
Lsp1109I GCAGC 8 cut(s) 24, 616, 710, 713, 890, 1069, 1351, 1471
LweI GCATC 5 cut(s) 148, 616, 1287, 1543, 1795
MabI ACCWGGT 1 cut(s) 1458
MaeI CTAG 4 cut(s) 123, 590, 1404, 1922
MaeII ACGT 8 cut(s) 128, 722, 907, 1108, 1354, 1378, 1477, 1640
MaeIII GTNAC 8 cut(s) 718, 898, 908, 1071, 1379, 1462, 1656, 1687
MalI GATC 9 cut(s) 57, 65, 210, 384, 497, 893, 1307, 1408, 1803
MboI GATC 9 cut(s) 55, 63, 208, 382, 495, 891, 1305, 1406, 1801
MboII GAAGA 8 cut(s) 385, 439, 778, 983, 1145, 1220, 1379, 1978
MfeI CAATTG 3 cut(s) 1301, 1770, 1990
MhlI GDGCHC 1 cut(s) 320
MlsI TGGCCA 1 cut(s) 639
MluNI TGGCCA 1 cut(s) 639
MlyI GAGTC 4 cut(s) 1084, 1163, 1523, 1829
MmeI TCCRAC 2 cut(s) 853, 1259
Mox20I TGGCCA 1 cut(s) 639
Mph1103I ATGCAT 1 cut(s) 1455
MroXI GAANNNNTTC 1 cut(s) 990
MscI TGGCCA 1 cut(s) 639
MseI TTAA 9 cut(s) 87, 339, 750, 800, 1010, 1041, 1208, 1599, 1926
MslI CAYNNNNRTG 1 cut(s) 1910
Msp20I TGGCCA 1 cut(s) 639
MspA1I CMGCKG 1 cut(s) 881
MspCI CTTAAG 1 cut(s) 86
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 2 cut(s) 32, 1460
MunI CAATTG 3 cut(s) 1301, 1770, 1990
Mva1269I GAATGC 1 cut(s) 23
MvaI CCWGG 1 cut(s) 1460
MwoI GCNNNNNNNGC 5 cut(s) 143, 831, 920, 1481, 1980
NciI CCSGG 1 cut(s) 32
NcoI CCATGG 1 cut(s) 452
NdeII GATC 9 cut(s) 55, 63, 208, 382, 495, 891, 1305, 1406, 1801
NlaIV GGNNCC 1 cut(s) 1919
NmuCI GTSAC 5 cut(s) 718, 898, 908, 1379, 1656
NsiI ATGCAT 1 cut(s) 1455
NspI RCATGY 4 cut(s) 617, 1004, 1031, 1548
PaeR7I CTCGAG 1 cut(s) 405
PagI TCATGA 1 cut(s) 1390
PceI AGGCCT 1 cut(s) 860
PciI ACATGT 3 cut(s) 613, 1027, 1544
PctI GAATGC 1 cut(s) 23
PdmI GAANNNNTTC 1 cut(s) 990
PfeI GAWTC 3 cut(s) 713, 1333, 1558
PkrI GCNGC 8 cut(s) 39, 631, 700, 703, 880, 1084, 1341, 1486
PleI GAGTC 4 cut(s) 1084, 1163, 1523, 1829
PmaCI CACGTG 1 cut(s) 908
PmlI CACGTG 1 cut(s) 908
PpsI GAGTC 4 cut(s) 1084, 1163, 1523, 1829
Ppu21I YACGTR 1 cut(s) 908
PscI ACATGT 3 cut(s) 613, 1027, 1544
PsiI TTATAA 1 cut(s) 951
Psp124BI GAGCTC 1 cut(s) 320
Psp1406I AACGTT 2 cut(s) 128, 1640
Psp6I CCWGG 1 cut(s) 1458
PspCI CACGTG 1 cut(s) 908
PspEI GGTNACC 1 cut(s) 1071
PspGI CCWGG 1 cut(s) 1458
PspN4I GGNNCC 1 cut(s) 1919
PspPI GGNCC 1 cut(s) 488
PstI CTGCAG 2 cut(s) 858, 1489
PstNI CAGNNNCTG 1 cut(s) 202
PvuII CAGCTG 1 cut(s) 881
RsaI GTAC 6 cut(s) 74, 403, 580, 1445, 1543, 1953
RsaNI GTAC 6 cut(s) 73, 402, 579, 1444, 1542, 1952
RseI CAYNNNNRTG 1 cut(s) 1910
SacI GAGCTC 1 cut(s) 320
SalI GTCGAC 1 cut(s) 95
SaqAI TTAA 9 cut(s) 87, 339, 750, 800, 1010, 1041, 1208, 1599, 1926
SatI GCNGC 8 cut(s) 38, 630, 699, 702, 879, 1083, 1340, 1485
Sau3AI GATC 9 cut(s) 55, 63, 208, 382, 495, 891, 1305, 1406, 1801
Sau96I GGNCC 1 cut(s) 488
ScaI AGTACT 1 cut(s) 403
SchI GAGTC 4 cut(s) 1084, 1163, 1523, 1829
ScrFI CCNGG 2 cut(s) 32, 1460
SduI GDGCHC 1 cut(s) 320
SexAI ACCWGGT 1 cut(s) 1458
SfaNI GCATC 5 cut(s) 148, 616, 1287, 1543, 1795
SfcI CTRYAG 2 cut(s) 854, 1485
Sfr274I CTCGAG 1 cut(s) 405
SinI GGWCC 1 cut(s) 488
SlaI CTCGAG 1 cut(s) 405
SmiMI CAYNNNNRTG 1 cut(s) 1910
SmlI CTYRAG 4 cut(s) 41, 86, 389, 405
SmoI CTYRAG 4 cut(s) 41, 86, 389, 405
SseBI AGGCCT 1 cut(s) 860
SsiI CCGC 4 cut(s) 53, 146, 274, 1337
SspMI CTAG 4 cut(s) 123, 590, 1404, 1922
SstI GAGCTC 1 cut(s) 320
StuI AGGCCT 1 cut(s) 860
StyD4I CCNGG 2 cut(s) 30, 1458
StyI CCWWGG 1 cut(s) 452
TaaI ACNGT 6 cut(s) 70, 198, 1077, 1262, 1794, 1958
TaiI ACGT 8 cut(s) 131, 725, 910, 1111, 1357, 1381, 1480, 1643
TaqI TCGA 3 cut(s) 96, 385, 406
TatI WGTACW 5 cut(s) 72, 401, 1443, 1541, 1951
TfiI GAWTC 3 cut(s) 713, 1333, 1558
Tru1I TTAA 9 cut(s) 87, 339, 750, 800, 1010, 1041, 1208, 1599, 1926
Tru9I TTAA 9 cut(s) 87, 339, 750, 800, 1010, 1041, 1208, 1599, 1926
TscAI CASTG 4 cut(s) 73, 203, 858, 1905
TseFI GTSAC 5 cut(s) 718, 898, 908, 1379, 1656
TseI GCWGC 8 cut(s) 37, 629, 698, 701, 878, 1082, 1339, 1484
Tsp45I GTSAC 5 cut(s) 718, 898, 908, 1379, 1656
TspDTI ATGAA 5 cut(s) 522, 1299, 1379, 1485, 1658
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 4 cut(s) 73, 203, 858, 1905
Vha464I CTTAAG 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 488
XapI RAATTY 1 cut(s) 1286
XbaI TCTAGA 1 cut(s) 1403
XceI RCATGY 4 cut(s) 617, 1004, 1031, 1548
XhoI CTCGAG 1 cut(s) 405
XmiI GTMKAC 2 cut(s) 96, 1122
XmnI GAANNNNTTC 1 cut(s) 990
XspI CTAG 4 cut(s) 123, 590, 1404, 1922
ZrmI AGTACT 1 cut(s) 403
Zsp2I ATGCAT 1 cut(s) 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.