RchiOBHm_Chr5g0041691

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000070
Physical Location & Seq
Reverse (-)
36599069 .. 36601964
2896 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32012

Sequence Viewer

Length: 2856 bp
ATGCTTTCAGAAAACAACTCTATACTAGTTTTGATTGCATTGCTTGTTCCTCTGTCATCTTCTTTGTCACTCTCACAAATCACAACTTTGATTGCTTCCTTCCTGAAACTAACTATGGCAGTGGGAGAGGTCTTTCTTGCTGCGTTCCTTCAGGTGCTATTCGACAGGTTAGCCTCTCGTGAGTTCCTCAAATTTGCACGGCAGAAGGGCATTGGCGGGAAGCTTGAAAAATGGAGTGAAACACTGTCTGCAATTCGAGCAGTGTTGTATGACGCCGAGGAGAAGCAACTCATAAGCATGTCAGTGAAAGAGTGGCTGGATGATCTCAAAGACTTGGCTTATGATGTGGAGGACATCTTGGACAGGTTTTCCACTGACATGTTACGATTCAAGGCCAAGGAGCAAAATCTGGCTGGCACAAGCAAGGTACGAGGCCTCTTTCTTAAAGTTAAATGCAACTTCAATATGAACTCTGAAATGGAAAAGATTTCTGAACGATTGCAAGAAATATCTCAACGAAAAAACATGCTCGGTTTGAATAATAGCGGAGCTGGATCTAGTGCGGCATCCCAAAGGCGAACAAGCTCATGCTTGCTTGATGGACCTGTGGTTGGAAGGTATGAAGACAAAAGGAAGATTGTGGCTTTGTTGTCAAGAGATGAACCTAGTACTGTCAATTTCCATGTAGTTGCAATTGTTGGCATGCCTGGAGTTGGGAAGACTACACTTGCTAAATCAGTATTCAAGGACAACAATATTGCCCTGGAACAGTTCGATCTTAAGGTATGGGTATCAGTGTCTGATGATTTTGACCTTGTTAGAGTAACAAAAGCCATTCTTGAATCGGTCACGTCTGTGTATTGTGACTTCAAGGAATTCAATAAAGTTCAAGATAACTTGAGCAAGGAGTTAGCAAGTAAAAAGTTTCTAATTGTTTTGGATGATGTTTGGAATACCTGCGACCATGATCAATGGACAACCCTACAGTCTCCCTTTCATGTCGGAGCACCAGGAAGTAAGATAATTGTAACGACTCGTGATGAAAAAGTTGCAAGAATGACGAGACCCATTGAGATCCACTGTTTGAAGTATATGTCAGATGATGATTGTTGGGAAGTCTTTCAGCAGCATGCATTCATGAATGTCAACAGTGGGAGACCACAAGGTTTTGAATTTTTTCGAGAGAGTATTGTTATAAAATGTGGCGGATTGCCCTTGGCTGCAAGGACTCTCGGTGGTCTTTTAGGTTGTGAAGACATAGATGAGTGGAAAAAAATATTGAACAATAGATTATGGAGTCTATCAAATAAGAGTCACATTCACCAAGTGTTGAAATTGAGCTATCACTATCTTCCTTCATATTTGAAAAGGTGCTTTGCATATTGCTCAATACTCCCAAACGACTATGAATTTGGGGAGAAGCAATTGACACTTCTTTGGATGGCAGAGGGTTTACTTCAACAACAAGAAGAACATGAAACATTAGAAGACTTGGGTGGTCACTATTTTGGACAGCTATTATCTAGGTCATTATTTCAAAAATCAAGCAGAAACAGTTCATTATATGTTATGCATCACCTTGTTGGTGATCTGGCTCGGTGGGCTGCGGGAGAAATTTGTCTCAGGTTGGAGGATAAAGATGGTAGATGTTCTCGAAAGACACGTCATTCGTCATACATTCCCGGTCAGTATGATGGGGTTAACAAATTTGAGGCATTCTCTGAAGCCAGACGTTTGCGTACTTTTCTACCATTGTCAGAAGACTTTTGTAACCATGTGACTTGTAAGGTTACTCTTGATTTACTGCCAAGATTGGAATACTTACGGGTACTCTCTTTGAATGGCTATCAAATAACTGAGTTGCCGAATTCAATTGGTAACTTGAAGCATCTACGGTACCTTGATGTTTCTCATACATTAATAATGAGATTGCCTGAATCAACAAGCACCCTTTGCAACTTACAGACATTAATATTAGAAAGTTGTTCTAGACTGAAGACACTGCCTACAAAGATGCGATATTTATCAAATCTACGCCATCTCAACAATTTAAATGTGCCTTCATTGGAAGGAATGCCCCCACAGCTAAGTCAACTGAGCAATCTGCAAACGTTGCCGAATTTCGTGGTGGGCAAAGGTAGTGACTCAAGGGTAAGAGAGATAGGGCCCCTGTCTCTTCTTCGAGGGACATTGTGCCTCTCAAGATTGGAAAATGTCAATGATGTTGAGGATGCAAGGAGGAGTGACTTAATTTGCAAGGAAGGGATTGATGCATTGCAGCTGGAATGGAATGATAAAGGAGAAAATGAATCGATCGTGCTTGACATGTTAAAACCTCATAAAAAGATGAAAGAGGTCATCATCAAGGGTTATGGAGGTTTGCAATTTTCAGCATGGATTGGAGATCCTTTATTCTCTTATGTGATGCATGTGAGGTTAGAGAATTGTAACAATTGTCGATTCTTGCCACCACTTGGACGATTGCCTTCTCTCCAAATACTTTGTATTAGAAATATGTCTGGGGTGGAAAAAGTTGGCCTAGAGTTTTGTGGAGAGGGTAGCTTGTCTTTTCCATTATTGGAGACCCTTGTGTTTGAGGATATGCAAAACTGGAATGAGTGGTTTCCTTGCCAAGGATATCAAGGACTTGAAGTTTTCCCTTCCTTGAAAATACTTTCAGTGAAAAGATGTCCCAAATTAAAGGGTGAGTTACCTCACAAGCTTGATTTGTTAACAAAGCTTGAGATTCATGAATGTAATGAACTGGTGGTTTCAATTGCCAACTACAAACAGCTCTGTGAATTAAACGTGCGCGATTGCAAAGGGGTGGTGTATAAAAGTATTTTTCAGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

951

Amino Acids

108.04

Weight (kDa)

7.46

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 46 - 136 4.1e-21 Rx N-terminal domain
NB-ARC PF00931 210 - 381 6e-37 NB-ARC domain
WHD_DRP PF23559 464 - 532 1.4e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 600 - 880 8.1e-23 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 719 - 839 1.8e-29 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1196
Acc36I ACCTGC 1 cut(s) 965
Acc65I GGTACC 1 cut(s) 1896
AccB1I GGYRCC 1 cut(s) 1896
AccB7I CCANNNNNTGG 1 cut(s) 2474
AccII CGCG 1 cut(s) 2814
AciI CCGC 5 cut(s) 216, 546, 563, 1206, 1607
AclI AACGTT 1 cut(s) 2111
AclWI GGATC 3 cut(s) 562, 1069, 2399
AcsI RAATTY 8 cut(s) 191, 875, 1172, 1409, 1614, 1706, 1867, 2119
AcuI CTGAAG 3 cut(s) 134, 1743, 2015
AcyI GRCGYC 1 cut(s) 273
AdeI CACNNNGTG 1 cut(s) 1327
AfaI GTAC 5 cut(s) 429, 670, 1741, 1830, 1898
AfiI CCNNNNNNNGG 4 cut(s) 611, 713, 2474, 2633
AflII CTTAAG 1 cut(s) 779
AflIII ACRYGT 3 cut(s) 378, 1661, 2325
AhlI ACTAGT 1 cut(s) 25
AjiI CACGTC 2 cut(s) 852, 1664
AjnI CCWGG 3 cut(s) 706, 762, 1009
AleI CACNNNNGTG 1 cut(s) 854
Alw21I GWGCWC 1 cut(s) 1009
Alw26I GTCTC 6 cut(s) 993, 1057, 1150, 1625, 2178, 2576
AlwI GGATC 3 cut(s) 562, 1069, 2399
AlwNI CAGNNNCTG 1 cut(s) 800
AoxI GGCC 4 cut(s) 393, 433, 2165, 2536
ApaI GGGCCC 1 cut(s) 2169
ApeKI GCWGC 5 cut(s) 140, 1126, 1220, 1604, 2278
ApoI RAATTY 8 cut(s) 191, 875, 1172, 1409, 1614, 1706, 1867, 2119
ArsI GACNNNNNNTTYG 6 cut(s) 1394, 1426, 1648, 1651, 1680, 1683
AseI ATTAAT 2 cut(s) 1919, 1970
Asp700I GAANNNNTTC 3 cut(s) 1119, 1176, 1555
Asp718I GGTACC 1 cut(s) 1896
AspLEI GCGC 1 cut(s) 2814
AspS9I GGNCC 3 cut(s) 602, 2165, 2166
AsuC2I CCSGG 1 cut(s) 1683
AsuHPI GGTGA 4 cut(s) 1313, 1568, 1598, 2717
AvaII GGWCC 1 cut(s) 602
BaeGI GKGCMC 1 cut(s) 2169
BanI GGYRCC 1 cut(s) 1896
BanII GRGCYC 1 cut(s) 2169
BarI GAAGNNNNNNTAC 2 cut(s) 851, 883
BauI CACGAG 2 cut(s) 177, 1035
BbsI GAAGAC 6 cut(s) 630, 725, 1260, 1494, 1767, 2003
Bbv12I GWGCWC 1 cut(s) 1009
BbvI GCAGC 5 cut(s) 127, 1138, 1207, 1591, 2290
BccI CCATC 5 cut(s) 593, 1435, 1634, 1688, 2046
BceAI ACGGC 1 cut(s) 215
BciT130I CCWGG 3 cut(s) 708, 764, 1011
BclI TGATCA 1 cut(s) 967
BcnI CCSGG 1 cut(s) 1683
BcoDI GTCTC 6 cut(s) 993, 1057, 1150, 1625, 2178, 2576
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 6 cut(s) 26, 558, 666, 1524, 1989, 2540
BfmI CTRYAG 1 cut(s) 983
BfrI CTTAAG 1 cut(s) 779
BfuAI ACCTGC 1 cut(s) 965
BisI GCNGC 6 cut(s) 141, 564, 1127, 1221, 1605, 2279
BlsI GCNGC 6 cut(s) 142, 565, 1128, 1222, 1606, 2280
BmcAI AGTACT 1 cut(s) 670
Bme1390I CCNGG 4 cut(s) 708, 764, 1011, 1683
Bme18I GGWCC 1 cut(s) 602
BmgBI CACGTC 2 cut(s) 852, 1664
BmgT120I GGNCC 3 cut(s) 602, 2165, 2166
BmiI GGNNCC 3 cut(s) 1898, 2167, 2168
BmrFI CCNGG 4 cut(s) 708, 764, 1011, 1683
BmsI GCATC 7 cut(s) 575, 1582, 1897, 2004, 2221, 2260, 2415
BpiI GAAGAC 6 cut(s) 630, 725, 1260, 1494, 1767, 2003
BplI GAGNNNNNCTC 2 cut(s) 1703, 1735
BpmI CTGGAG 1 cut(s) 729
BpuEI CTTGAG 4 cut(s) 919, 2131, 2185, 2762
BpuMI CCSGG 1 cut(s) 1683
Bsa29I ATCGAT 1 cut(s) 2312
BsaBI GATNNNNATC 1 cut(s) 2023
BsaHI GRCGYC 1 cut(s) 273
BsaI GGTCTC 3 cut(s) 1057, 1150, 2576
BsaJI CCNNGG 5 cut(s) 276, 396, 762, 1215, 2632
Bsc4I CCNNNNNNNGG 4 cut(s) 611, 713, 2474, 2633
Bse1I ACTGG 2 cut(s) 2615, 2769
Bse3DI GCAATG 2 cut(s) 38, 2273
Bse8I GATNNNNATC 1 cut(s) 2023
BseBI CCWGG 3 cut(s) 708, 764, 1011
BseCI ATCGAT 1 cut(s) 2312
BseDI CCNNGG 5 cut(s) 276, 396, 762, 1215, 2632
BseGI GGATG 5 cut(s) 325, 566, 946, 1446, 2236
BseJI GATNNNNATC 1 cut(s) 2023
BseLI CCNNNNNNNGG 4 cut(s) 611, 713, 2474, 2633
BseMI GCAATG 2 cut(s) 38, 2273
BseMII CTCAG 3 cut(s) 1636, 1848, 2087
BseNI ACTGG 2 cut(s) 2615, 2769
BseRI GAGGAG 2 cut(s) 293, 2254
BseSI GKGCMC 1 cut(s) 2169
BseXI GCAGC 5 cut(s) 127, 1138, 1207, 1591, 2290
Bsh1236I CGCG 1 cut(s) 2814
Bsh1285I CGRYCG 1 cut(s) 2316
BshFI GGCC 4 cut(s) 395, 435, 2167, 2538
BshNI GGYRCC 1 cut(s) 1896
BshVI ATCGAT 1 cut(s) 2312
BsiEI CGRYCG 1 cut(s) 2316
BsiHKAI GWGCWC 1 cut(s) 1009
BsiSI CCGG 1 cut(s) 1683
BslFI GGGAC 2 cut(s) 2200, 2676
BslI CCNNNNNNNGG 4 cut(s) 611, 713, 2474, 2633
BsmAI GTCTC 6 cut(s) 993, 1057, 1150, 1625, 2178, 2576
BsmFI GGGAC 2 cut(s) 2200, 2676
BsmI GAATGC 3 cut(s) 1133, 1715, 2079
BsnI GGCC 4 cut(s) 395, 435, 2167, 2538
Bso31I GGTCTC 3 cut(s) 1057, 1150, 2576
Bsp120I GGGCCC 1 cut(s) 2165
Bsp1286I GDGCHC 2 cut(s) 1009, 2169
Bsp143I GATC 8 cut(s) 322, 554, 775, 967, 1074, 1588, 2313, 2404
BspACI CCGC 5 cut(s) 216, 546, 563, 1206, 1607
BspANI GGCC 4 cut(s) 395, 435, 2167, 2538
BspCNI CTCAG 3 cut(s) 1635, 1849, 2088
BspDI ATCGAT 1 cut(s) 2312
BspFNI CGCG 1 cut(s) 2814
BspHI TCATGA 2 cut(s) 1137, 2749
BspLI GGNNCC 3 cut(s) 1898, 2167, 2168
BspMI ACCTGC 1 cut(s) 965
BspPI GGATC 3 cut(s) 562, 1069, 2399
BspT107I GGYRCC 1 cut(s) 1896
BspTI CTTAAG 1 cut(s) 779
BspTNI GGTCTC 3 cut(s) 1057, 1150, 2576
BsrDI GCAATG 2 cut(s) 38, 2273
BsrI ACTGG 2 cut(s) 2615, 2769
BssECI CCNNGG 5 cut(s) 276, 396, 762, 1215, 2632
BssMI GATC 8 cut(s) 322, 554, 775, 967, 1074, 1588, 2313, 2404
BssNI GRCGYC 1 cut(s) 273
BssSI CACGAG 2 cut(s) 177, 1035
BssT1I CCWWGG 3 cut(s) 396, 1215, 2632
Bst2BI CACGAG 2 cut(s) 177, 1035
Bst2UI CCWGG 3 cut(s) 708, 764, 1011
Bst4CI ACNGT 8 cut(s) 246, 673, 771, 987, 1082, 1151, 1556, 1896
Bst6I CTCTTC 1 cut(s) 2181
BstACI GRCGYC 1 cut(s) 273
BstAFI CTTAAG 1 cut(s) 779
BstAPI GCANNNNNTGC 2 cut(s) 1953, 2113
BstC8I GCNNGC 4 cut(s) 415, 593, 704, 1131
BstDEI CTNAG 4 cut(s) 1622, 1857, 2087, 2096
BstENI CCTNNNNNAGG 1 cut(s) 2631
BstF5I GGATG 5 cut(s) 325, 566, 946, 1446, 2236
BstFNI CGCG 1 cut(s) 2814
BstHHI GCGC 1 cut(s) 2814
BstKTI GATC 8 cut(s) 325, 557, 778, 970, 1077, 1591, 2316, 2407
BstMAI GTCTC 6 cut(s) 993, 1057, 1150, 1625, 2178, 2576
BstMBI GATC 8 cut(s) 322, 554, 775, 967, 1074, 1588, 2313, 2404
BstMCI CGRYCG 1 cut(s) 2316
BstMWI GCNNNNNNNGC 5 cut(s) 257, 1601, 1953, 2083, 2113
BstNI CCWGG 3 cut(s) 708, 764, 1011
BstNSI RCATGY 7 cut(s) 301, 382, 529, 706, 1133, 2329, 2432
BstSCI CCNGG 4 cut(s) 706, 762, 1009, 1681
BstSFI CTRYAG 1 cut(s) 983
BstSLI GKGCMC 1 cut(s) 2169
BstUI CGCG 1 cut(s) 2814
BstV1I GCAGC 5 cut(s) 127, 1138, 1207, 1591, 2290
BstV2I GAAGAC 6 cut(s) 630, 725, 1260, 1494, 1767, 2003
BstX2I RGATCY 3 cut(s) 554, 1074, 2404
BstYI RGATCY 3 cut(s) 554, 1074, 2404
Bsu15I ATCGAT 1 cut(s) 2312
BsuRI GGCC 4 cut(s) 395, 435, 2167, 2538
BsuTUI ATCGAT 1 cut(s) 2312
BtrI CACGTC 2 cut(s) 852, 1664
BtsCI GGATG 5 cut(s) 325, 566, 946, 1446, 2236
BtsI GCAGTG 3 cut(s) 126, 267, 2000
BveI ACCTGC 1 cut(s) 965
Cac8I GCNNGC 4 cut(s) 415, 593, 704, 1131
CaiI CAGNNNCTG 1 cut(s) 800
CciI TCATGA 2 cut(s) 1137, 2749
CfoI GCGC 1 cut(s) 2814
Cfr13I GGNCC 3 cut(s) 602, 2165, 2166
ClaI ATCGAT 1 cut(s) 2312
CseI GACGC 1 cut(s) 281
Csp6I GTAC 5 cut(s) 428, 669, 1740, 1829, 1897
CviQI GTAC 5 cut(s) 428, 669, 1740, 1829, 1897
DdeI CTNAG 4 cut(s) 1622, 1857, 2087, 2096
DpnI GATC 8 cut(s) 324, 556, 777, 969, 1076, 1590, 2315, 2406
DpnII GATC 8 cut(s) 322, 554, 775, 967, 1074, 1588, 2313, 2404
DraI TTTAAA 1 cut(s) 2052
DraIII CACNNNGTG 1 cut(s) 1327
Eam1104I CTCTTC 1 cut(s) 2181
EarI CTCTTC 1 cut(s) 2181
EciI GGCGGA 1 cut(s) 1221
Eco130I CCWWGG 3 cut(s) 396, 1215, 2632
Eco147I AGGCCT 1 cut(s) 435
Eco24I GRGCYC 1 cut(s) 2169
Eco31I GGTCTC 3 cut(s) 1057, 1150, 2576
Eco32I GATATC 1 cut(s) 2639
Eco47I GGWCC 1 cut(s) 602
Eco57I CTGAAG 3 cut(s) 134, 1743, 2015
EcoNI CCTNNNNNAGG 1 cut(s) 2631
EcoO109I RGGNCCY 2 cut(s) 2165, 2166
EcoRI GAATTC 2 cut(s) 875, 1867
EcoRII CCWGG 3 cut(s) 706, 762, 1009
EcoRV GATATC 1 cut(s) 2639
EcoT14I CCWWGG 3 cut(s) 396, 1215, 2632
EcoT22I ATGCAT 4 cut(s) 1135, 1575, 2275, 2430
EcoT38I GRGCYC 1 cut(s) 2169
ErhI CCWWGG 3 cut(s) 396, 1215, 2632
FalI AAGNNNNNCTT 2 cut(s) 822, 854
FaqI GGGAC 2 cut(s) 2200, 2676
FauI CCCGC 2 cut(s) 209, 1600
FbaI TGATCA 1 cut(s) 967
Fnu4HI GCNGC 6 cut(s) 141, 564, 1127, 1221, 1605, 2279
FokI GGATG 5 cut(s) 332, 553, 953, 1453, 2243
FriOI GRGCYC 1 cut(s) 2169
Fsp4HI GCNGC 6 cut(s) 141, 564, 1127, 1221, 1605, 2279
FspBI CTAG 6 cut(s) 26, 558, 666, 1524, 1989, 2540
GlaI GCGC 1 cut(s) 2813
GluI GCNGC 6 cut(s) 141, 564, 1127, 1221, 1605, 2279
GsuI CTGGAG 1 cut(s) 729
HaeIII GGCC 4 cut(s) 395, 435, 2167, 2538
HapII CCGG 1 cut(s) 1683
HgaI GACGC 1 cut(s) 281
HhaI GCGC 1 cut(s) 2814
Hin1I GRCGYC 1 cut(s) 273
Hin6I GCGC 1 cut(s) 2812
HinP1I GCGC 1 cut(s) 2812
HincII GTYRAC 4 cut(s) 1147, 1702, 2093, 2733
HindII GTYRAC 4 cut(s) 1147, 1702, 2093, 2733
HindIII AAGCTT 3 cut(s) 221, 2720, 2738
HpaI GTTAAC 2 cut(s) 1702, 2733
HpaII CCGG 1 cut(s) 1683
HphI GGTGA 4 cut(s) 1313, 1568, 1598, 2717
Hpy166II GTNNAC 5 cut(s) 1147, 1454, 1702, 2093, 2733
Hpy188I TCNGA 8 cut(s) 10, 475, 493, 802, 1004, 1099, 1723, 1759
Hpy8I GTNNAC 5 cut(s) 1147, 1454, 1702, 2093, 2733
HpyCH4III ACNGT 8 cut(s) 246, 673, 771, 987, 1082, 1151, 1556, 1896
HpyCH4IV ACGT 5 cut(s) 851, 1663, 1732, 2111, 2808
HpyF10VI GCNNNNNNNGC 5 cut(s) 257, 1601, 1953, 2083, 2113
HpyF3I CTNAG 4 cut(s) 1622, 1857, 2087, 2096
HpySE526I ACGT 5 cut(s) 851, 1663, 1732, 2111, 2808
Hsp92I GRCGYC 1 cut(s) 273
HspAI GCGC 1 cut(s) 2812
KpnI GGTACC 1 cut(s) 1900
Ksp22I TGATCA 1 cut(s) 967
KspAI GTTAAC 2 cut(s) 1702, 2733
Kzo9I GATC 8 cut(s) 322, 554, 775, 967, 1074, 1588, 2313, 2404
LmnI GCTCC 3 cut(s) 400, 548, 1004
Lsp1109I GCAGC 5 cut(s) 127, 1138, 1207, 1591, 2290
LweI GCATC 7 cut(s) 575, 1582, 1897, 2004, 2221, 2260, 2415
MaeI CTAG 6 cut(s) 26, 558, 666, 1524, 1989, 2540
MaeII ACGT 5 cut(s) 851, 1663, 1732, 2111, 2808
MalI GATC 8 cut(s) 324, 556, 777, 969, 1076, 1590, 2315, 2406
MboI GATC 8 cut(s) 322, 554, 775, 967, 1074, 1588, 2313, 2404
MfeI CAATTG 5 cut(s) 693, 1424, 1872, 2452, 2775
MflI RGATCY 3 cut(s) 554, 1074, 2404
MhlI GDGCHC 2 cut(s) 1009, 2169
MlyI GAGTC 5 cut(s) 1027, 1222, 1306, 1321, 2138
MmeI TCCRAC 3 cut(s) 592, 982, 1608
Mph1103I ATGCAT 4 cut(s) 1135, 1575, 2275, 2430
MroXI GAANNNNTTC 3 cut(s) 1119, 1176, 1555
MslI CAYNNNNRTG 4 cut(s) 296, 302, 377, 854
MspA1I CMGCKG 1 cut(s) 2281
MspCI CTTAAG 1 cut(s) 779
MspI CCGG 1 cut(s) 1683
MspR9I CCNGG 4 cut(s) 708, 764, 1011, 1683
MunI CAATTG 5 cut(s) 693, 1424, 1872, 2452, 2775
Mva1269I GAATGC 3 cut(s) 1133, 1715, 2079
MvaI CCWGG 3 cut(s) 708, 764, 1011
MvnI CGCG 1 cut(s) 2814
MwoI GCNNNNNNNGC 5 cut(s) 257, 1601, 1953, 2083, 2113
NciI CCSGG 1 cut(s) 1683
NdeII GATC 8 cut(s) 322, 554, 775, 967, 1074, 1588, 2313, 2404
NlaIV GGNNCC 3 cut(s) 1898, 2167, 2168
NmeAIII GCCGAG 1 cut(s) 301
NmuCI GTSAC 8 cut(s) 66, 847, 863, 1313, 1499, 1777, 2141, 2243
NsiI ATGCAT 4 cut(s) 1135, 1575, 2275, 2430
NspI RCATGY 7 cut(s) 301, 382, 529, 706, 1133, 2329, 2432
OliI CACNNNNGTG 1 cut(s) 854
PaeI GCATGC 2 cut(s) 706, 1133
PagI TCATGA 2 cut(s) 1137, 2749
PceI AGGCCT 1 cut(s) 435
PciI ACATGT 2 cut(s) 378, 2325
PcsI WCGNNNNNNNCGW 1 cut(s) 1660
PctI GAATGC 3 cut(s) 1133, 1715, 2079
PdmI GAANNNNTTC 3 cut(s) 1119, 1176, 1555
PfeI GAWTC 6 cut(s) 387, 842, 1937, 2309, 2460, 2746
PflMI CCANNNNNTGG 1 cut(s) 2474
PkrI GCNGC 6 cut(s) 142, 565, 1128, 1222, 1606, 2280
Ple19I CGATCG 1 cut(s) 2316
PleI GAGTC 5 cut(s) 1027, 1222, 1305, 1320, 2138
PpsI GAGTC 5 cut(s) 1027, 1222, 1305, 1320, 2138
PscI ACATGT 2 cut(s) 378, 2325
PshBI ATTAAT 2 cut(s) 1919, 1970
PsiI TTATAA 1 cut(s) 1196
Psp1406I AACGTT 1 cut(s) 2111
Psp6I CCWGG 3 cut(s) 706, 762, 1009
PspGI CCWGG 3 cut(s) 706, 762, 1009
PspN4I GGNNCC 3 cut(s) 1898, 2167, 2168
PspOMI GGGCCC 1 cut(s) 2165
PspPI GGNCC 3 cut(s) 602, 2165, 2166
PstNI CAGNNNCTG 1 cut(s) 800
PsuI RGATCY 3 cut(s) 554, 1074, 2404
PvuI CGATCG 1 cut(s) 2316
PvuII CAGCTG 1 cut(s) 2281
RsaI GTAC 5 cut(s) 429, 670, 1741, 1830, 1898
RsaNI GTAC 5 cut(s) 428, 669, 1740, 1829, 1897
RseI CAYNNNNRTG 4 cut(s) 296, 302, 377, 854
SatI GCNGC 6 cut(s) 141, 564, 1127, 1221, 1605, 2279
Sau3AI GATC 8 cut(s) 322, 554, 775, 967, 1074, 1588, 2313, 2404
Sau96I GGNCC 3 cut(s) 602, 2165, 2166
ScaI AGTACT 1 cut(s) 670
SchI GAGTC 5 cut(s) 1027, 1222, 1306, 1321, 2138
ScrFI CCNGG 4 cut(s) 708, 764, 1011, 1683
SduI GDGCHC 2 cut(s) 1009, 2169
SfaNI GCATC 7 cut(s) 575, 1582, 1897, 2004, 2221, 2260, 2415
SfcI CTRYAG 1 cut(s) 983
SinI GGWCC 1 cut(s) 602
SmiI ATTTAAAT 1 cut(s) 2052
SmiMI CAYNNNNRTG 4 cut(s) 296, 302, 377, 854
SmlI CTYRAG 5 cut(s) 779, 898, 2146, 2200, 2741
SmoI CTYRAG 5 cut(s) 779, 898, 2146, 2200, 2741
SpeI ACTAGT 1 cut(s) 25
SphI GCATGC 2 cut(s) 706, 1133
SseBI AGGCCT 1 cut(s) 435
SsiI CCGC 5 cut(s) 216, 546, 563, 1206, 1607
SspI AATATT 3 cut(s) 757, 1278, 1974
SspMI CTAG 6 cut(s) 26, 558, 666, 1524, 1989, 2540
StuI AGGCCT 1 cut(s) 435
StyD4I CCNGG 4 cut(s) 706, 762, 1009, 1681
StyI CCWWGG 3 cut(s) 396, 1215, 2632
SwaI ATTTAAAT 1 cut(s) 2052
TaaI ACNGT 8 cut(s) 246, 673, 771, 987, 1082, 1151, 1556, 1896
TaiI ACGT 5 cut(s) 854, 1666, 1735, 2114, 2811
TaqI TCGA 8 cut(s) 162, 256, 774, 1180, 1654, 2182, 2312, 2458
TaqII GACCGA 1 cut(s) 835
TatI WGTACW 1 cut(s) 668
TauI GCSGC 1 cut(s) 566
TfiI GAWTC 6 cut(s) 387, 842, 1937, 2309, 2460, 2746
TseFI GTSAC 8 cut(s) 66, 847, 863, 1313, 1499, 1777, 2141, 2243
TseI GCWGC 5 cut(s) 140, 1126, 1220, 1604, 2278
Tsp45I GTSAC 8 cut(s) 66, 847, 863, 1313, 1499, 1777, 2141, 2243
Van91I CCANNNNNTGG 1 cut(s) 2474
Vha464I CTTAAG 1 cut(s) 779
VpaK11BI GGWCC 1 cut(s) 602
VspI ATTAAT 2 cut(s) 1919, 1970
XagI CCTNNNNNAGG 1 cut(s) 2631
XapI RAATTY 8 cut(s) 191, 875, 1172, 1409, 1614, 1706, 1867, 2119
XbaI TCTAGA 1 cut(s) 1988
XceI RCATGY 7 cut(s) 301, 382, 529, 706, 1133, 2329, 2432
XmnI GAANNNNTTC 3 cut(s) 1119, 1176, 1555
XspI CTAG 6 cut(s) 26, 558, 666, 1524, 1989, 2540
ZrmI AGTACT 1 cut(s) 670
Zsp2I ATGCAT 4 cut(s) 1135, 1575, 2275, 2430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.