Rw1G003940

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000070
Physical Location & Seq
Forward (+)
7406655 .. 7417937
11283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G003940.1

Sequence Viewer

Length: 4047 bp
ATGGTAGCGGAGGTCTTCCTCGGTGCATTCCTTCAGTTACTGCTCGACAGGTTAACTCCTCATCACGATTTGCTCAACTTGGCGTGCCTGCATGGCGTCGACAAGAAACTCAAGAAATGGAGTGCAGCGCTGTCTGCAATCGGAGCGGTGCTGCAGGACGCGGAGGACAAGCAACTGACGAGCGAGGCTGTGAAGCTATGGCTGGATGAACTCAAACACTTGGCTTTTGACATGGATGACATCTTGGACACCTTTTCCACCGAGCTGTTGCGGCGCAACGTGATGAATCAGCAACATGCGTGGCTTACAAGTAAGGTACGGGGCCTACTTTCTAGAGCTAAGTTCAATTTCAGTATGAATTCGGAGATCGAGGAGATTAGTGATAGGTTGGTAGAGATATCTGAAAGAAAGGACTTGCTTGGGTTGAATTATGTTGTGAATAAATGTAGTCAGGTGTGGCAAAGGCCGCCGAGTTCATGTGTGTTAGATGGGCCTGTGGTTGGTAGGGATGATGATAAAAGGAAGATTGTTGAGGTGTTATTGAGGGATGATCAGCCTAGTAGTGTCAATTTCCATGTGGTTACCATTGTGGGTATGCCAGGACTTGGAAAGACTACAATTGCAGGTCATGTCTTCAATGATGATGCAATGGAACGGTTTAGTCCCAAAGTATGGGTATCTGTGTCTGATAACTTCAATCTTGTGAGAGTGACAAAGGCAATTCTGGAATCAGTTACATCTGCAAGCTGTGATTTGGAGGAGTTCAGTCAAATTCAGGAGAGTCTGAGCAAGGCATTGGCAAGTAAAAAGTTTTTGGTTGTTTTAGATGATGTCTGGAACACATGTGACTATGATCTATGGGCAAAGCTGCAGTCTGCCTTTCGTGGCGGAGCTTTGGGAAGTAAGGTAATTGTGACAACTCGTGACACACAAGTTGCTAAGATGATGAGATCCATTGAAGTTCATAACTTGGGGTGCATGTCAGATGATGATTGCTGGGAAGTCTTTGGGCAACATGCATTCTTGAATGTTGAGAATGGGAGACTGCAAAGCTTTGAGTTGTTTCGGGAGAAAGTTGTAGCAAAATGTGGTGGGTTGCCCTTGGCTGCAAGAGTCCTTGGTGGTCTTTTAGGCTGTAAAGAGATAGACGAATGGGAAGAAATATTGAACAGTAAATTATGGAGTCTCTATCACTATCTCTCTTCAAATTTGAAAAGGTGCTTTGCCTATTGCTCAATACTTCCAAATGACTATGAATTTGGGGAGAGGCAGTTGATCCTTCTATGGATGGCAGAGGGTCTCATTCAGCAGTCACATGAATGTAAACAACTGGAAGATTTAGGGAGTAACTATTTTCGGGAGCTATTGTCCAGGTCATTGTTTCAAAGGTCCAGTAAAAACCATTCACAATTTGTTATGCATGACCTTGTTGGTGATTTGTCACGGTGGGCTGCAGGAGACATGTGTTTCAGATTGGAGGATAAGCTGGATGTTAGATGTTCTTCAAAGGTTCGCCATTCCTCTTACATTCCTGGTCAATTTGATGGTGTTCAAAAATTCAAAGAATTTTTTGAAGTCAGGCATTTGCGGACTTTCTTACCACTTTCACTTTCAGGTCGATCAAATTATCTAACTCGTAATGTTACTTTTGATCTATTACCAAAGTTGAAGTACTTGAGGGCACTCTCTTTTAATGGCTATCAAATAACTGAGTTACCCAATTCTATTGGTAAGCTGAAGCATCTGCGGTACCTTGATCTTTCTCACACACAGATAACAACTTTGCCAGAATCAACCAGCAATCTGTACAACTTACAGACATTGATATTAGAAAATTGTGCTTGTTTGAAGGCACTTCCTGCAGACATGAGGAATCTAACTGATCTGCGGCACCTTAACAATTCAGATGTACCTTCATTGGAAGAAATGCCTGTGAAACTAGGTAGATTGACTAATCTTCAAACACTGAGTACTTTTGTGCTGGGCAAAGGTAGCAAGTCAGGAATAAGTGAGATAGGTCCTTTATTGTGTCTCCAAGGGACATTGTGCCTCTCAAGATTGGAGAATGTGACTGATGTTGATGATGCAAGGAGGGCTGACTTGACTAGCAAGGAAGGGATTGATTCATTGCAACTAGAATGGAGAGAATCAGGAGAAAGGGAATCAGATGTTTTTGAAATGTTAAAACCTCATAGGAAGCTCAAAGTGCTCACTGTAAAAGGTTATGGAGGTCTTACATTTTCAACATGGACTGGACACCCTTCATTCTCTTATATGACGCATGTAAAGTTGGAACATTGTAAAAATTGTCGATTCTTGCCTCCACTTGGACAGTTGTCTTCTCTGAAAGAACTTTGTATAAAAGGAATGTCTACAGTGGAAAGCGTGGGTCTTGAGTTTTATGGAGAGAGTAGCCTGCCTTTTCCTGTATTGGAGACCCTGGAGTTCAAGGATATGCAGAAATGGAGGGAGTGGCTTCCTCGGGTGCAAGATCAAGGAATTGAAGTTTTCCCTTGCTTGAAAATGGTTTGTATCTCTGGATGCCCCATACTGGAGGGGAGATTATTACCTGAAAACCTTGATCTATTATCGAAGCTTGCCATTGATGGATGCGGCCAATTGGTGGTTTCAATTGCCAGTTATAAACGTCTTCGTGATTTGAAAATTCATGCTTGCAAAATGCTGGTACATACAAGTGGGGTTCAATTTGAGTTACTAGAGGCGATGGAGCTTTCATGTATTTCAGAGTTTAGACTCCACATGGAACGATTCAAGAGAGACTTAACAAAGCTTAAAAATCTGAGAATTACTAGTTGTGAGGAGCTGACATCTTTATGGAAAAATGAGGATAGATTGCTGCAACGTCCGATTTATGAAGGAAACAGCTCTCACTCTTACTTTGTTCAAGAGCTGAAGTCACTTCAATGTCTTCACATAAATGATTGCTCAAATCTAGTTTCTTTTTCAAAGGTTAGCCTGCCACCTTCTCTTAAAGAAATAAAGATTGAGCGGTGCAGTTCTCTTATAAGATACCAGATACCCCCAAAAGTAAGAAGTCTATACATCGATTCCTGCATAAACTTGGAATCAGTAGTGGGGGAGGACAAGGAAGGTGAGGATCCTTCATCTTCCTTTTCTCCTTGTTTGATACAAGAGGAGGCATCCTGCCTCGAGTACTTGAGCATAGTGAATTGTATGAGATTTACAACCTTATCATCCACAGGCCAGCTAGCCAGGGGACTTAAACATATTATCATAAATCATTGTCACCAGCTAGAATCAATAACTGATAGCTTCCACGTTAACACCTTGCTTGAAGAAATTGTGATTAGGAGTTGTGGAAATCTTAAATCGTTACCAGAGGGCTTATGTGACCTCACCAATCTTCAAGTCTTTAAGATTTGGGACTGTGAAAGTCTTGTTTCTTTCCCAAGAGGAGGGTTGCCAACAAGAACCTCCAGCTTGAAAGAGATCGAGGTTATGAATTGTGATAACTTACAGGCCCTTCCTGAAGACATGCAGAACCTCCACTGTCTTCAGAGTTTGAGGATTTCATACTGTGAAGGTTTCGCTCCATTTCTAGACAAGGGTTTGCCACCCAACCTAACATTCCTTGAGATTCGAAATCTCAACAGTTGTAGGCCACTCCTGGAAGCTCAAGGATTGCACAGACTCACTTCTCTTAGAGCGTTGGATATCAAGGGAAATGATTCACAACTAGTGTCATTTCCACCTGACAAGGAGATGGTGCTCCCCAAATCTCTCATTCTACTTCATATTGCAGGGTTCCCGAATCTGAGGCACCTATCCAGCAAGGGCTTTCAGTTCCTCCCCTCTCTTGAATCTCTTCAAGTCTTCAGCTGTCCAAAGCTAGCATCCATTCCTGAGGAGGGACTCCCTCTTTCACTTACACAACTTCATATCTTTGGTTGTCCACTGCTAGTAAAGAACTGCAGACCTGGAAAAGGACAATACTGGCACTCCATAGCTCGCATCCCTTTCATATGGATTAAGGGTGATGAGGGAGCAGACATATTTAGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1348

Amino Acids

152.56

Weight (kDa)

6.32

Isoelectric Point (pI)

48.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 7 - 97 2.9e-20 Rx N-terminal domain
NB-ARC PF00931 170 - 341 5.4e-37 NB-ARC domain
WHD_DRP PF23559 413 - 480 4.4e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 553 - 817 5.4e-22 Leucine-rich repeat region
LRR_8 PF13855 560 - 613 7.3e-06 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 669 - 790 3.3e-33 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2643, 3026
Acc36I ACCTGC 1 cut(s) 614
Acc65I GGTACC 1 cut(s) 1749
AccB1I GGYRCC 3 cut(s) 1749, 1890, 3801
AccB7I CCANNNNNTGG 3 cut(s) 605, 672, 2623
AccBSI CCGCTC 2 cut(s) 146, 3010
AccI GTMKAC 2 cut(s) 99, 2372
AccII CGCG 1 cut(s) 161
AclWI GGATC 4 cut(s) 945, 1270, 3113, 3126
AcoI YGGCCR 1 cut(s) 2614
AcsI RAATTY 7 cut(s) 358, 771, 1207, 1256, 1556, 1565, 2664
AcuI CTGAAG 6 cut(s) 17, 1757, 2933, 3521, 3531, 3841
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 8 cut(s) 318, 1673, 1751, 1808, 1911, 1972, 2688, 3176
AfeI AGCGCT 1 cut(s) 129
AfiI CCNNNNNNNGG 9 cut(s) 500, 605, 672, 2327, 2551, 2623, 3437, 3890, 3965
AflIII ACRYGT 2 cut(s) 842, 1461
AhlI ACTAGT 2 cut(s) 2810, 3718
AjnI CCWGG 7 cut(s) 598, 1370, 1531, 2439, 3233, 3648, 3958
AjuI GAANNNNNNNTTGG 2 cut(s) 3593, 3625
Alw21I GWGCWC 2 cut(s) 2211, 3753
Alw26I GTCTC 7 cut(s) 1036, 1190, 1304, 1452, 2036, 2429, 2772
AlwI GGATC 4 cut(s) 945, 1270, 3113, 3126
AlwNI CAGNNNCTG 1 cut(s) 40
Ama87I CYCGRG 2 cut(s) 2481, 3170
Aor51HI AGCGCT 1 cut(s) 129
AoxI GGCC 7 cut(s) 322, 464, 491, 2614, 3223, 3501, 3641
ApeKI GCWGC 6 cut(s) 125, 151, 868, 1106, 1451, 2857
ApoI RAATTY 7 cut(s) 358, 771, 1207, 1256, 1556, 1565, 2664
ArsI GACNNNNNNTTYG 4 cut(s) 857, 889, 1241, 1273
Asp700I GAANNNNTTC 2 cut(s) 3709, 3846
Asp718I GGTACC 1 cut(s) 1749
AspLEI GCGC 2 cut(s) 130, 276
AspS9I GGNCC 5 cut(s) 322, 491, 1389, 2018, 3502
AsuHPI GGTGA 5 cut(s) 1445, 3125, 3260, 3370, 4028
AsuII TTCGAA 1 cut(s) 3622
AsuNHI GCTAGC 2 cut(s) 3229, 3871
AvaI CYCGRG 2 cut(s) 2481, 3170
AvaII GGWCC 2 cut(s) 1389, 2018
AxyI CCTNAGG 1 cut(s) 3885
BaeGI GKGCMC 1 cut(s) 1684
BamHI GGATCC 1 cut(s) 3118
BanI GGYRCC 3 cut(s) 1749, 1890, 3801
BauI CACGAG 1 cut(s) 921
BbsI GAAGAC 8 cut(s) 7, 625, 2331, 2642, 2921, 3519, 3527, 3847
Bbv12I GWGCWC 2 cut(s) 2211, 3753
BbvI GCAGC 6 cut(s) 137, 138, 855, 1093, 1438, 2844
BccI CCATC 6 cut(s) 482, 1282, 1538, 2600, 2719, 3739
BciT130I CCWGG 7 cut(s) 600, 1372, 1533, 2441, 3235, 3650, 3960
BclI TGATCA 1 cut(s) 550
BcoDI GTCTC 7 cut(s) 1036, 1190, 1304, 1452, 2036, 2429, 2772
BcuI ACTAGT 2 cut(s) 2810, 3718
BfmI CTRYAG 6 cut(s) 152, 869, 1452, 1860, 2373, 3952
BfoI RGCGCY 1 cut(s) 131
BfuAI ACCTGC 1 cut(s) 614
BglI GCCNNNNNGGC 1 cut(s) 93
BmcAI AGTACT 3 cut(s) 1673, 1972, 3176
Bme1390I CCNGG 7 cut(s) 600, 1372, 1533, 2441, 3235, 3650, 3960
Bme18I GGWCC 2 cut(s) 1389, 2018
BmeT110I CYCGRG 2 cut(s) 2481, 3170
BmgT120I GGNCC 5 cut(s) 322, 491, 1389, 2018, 3502
BmiI GGNNCC 6 cut(s) 323, 1751, 1892, 3120, 3788, 3803
BmrFI CCNGG 7 cut(s) 600, 1372, 1533, 2441, 3235, 3650, 3960
BmsI GCATC 8 cut(s) 634, 1750, 2074, 2531, 2600, 3170, 3884, 4002
BmtI GCTAGC 2 cut(s) 3233, 3875
BoxI GACNNNNGTC 1 cut(s) 2335
BpiI GAAGAC 8 cut(s) 7, 625, 2331, 2642, 2921, 3519, 3527, 3847
BpmI CTGGAG 3 cut(s) 2462, 2573, 3442
Bpu14I TTCGAA 1 cut(s) 3622
BpuEI CTTGAG 7 cut(s) 95, 1696, 2038, 2414, 3199, 3635, 3642
Bsa29I ATCGAT 1 cut(s) 3066
BsaHI GRCGYC 1 cut(s) 96
BsaI GGTCTC 2 cut(s) 1304, 2429
BsaJI CCNNGG 7 cut(s) 19, 1101, 1117, 2035, 2439, 2480, 3234
BsaXI ACNNNNNCTCC 6 cut(s) 1256, 1286, 1352, 1382, 3965, 3995
Bsc4I CCNNNNNNNGG 9 cut(s) 500, 605, 672, 2327, 2551, 2623, 3437, 3890, 3965
Bse1I ACTGG 6 cut(s) 1335, 1392, 2257, 2556, 2637, 3980
Bse21I CCTNAGG 1 cut(s) 3885
Bse3DI GCAATG 2 cut(s) 654, 2126
BseBI CCWGG 7 cut(s) 600, 1372, 1533, 2441, 3235, 3650, 3960
BseCI ATCGAT 1 cut(s) 3066
BseDI CCNNGG 7 cut(s) 19, 1101, 1117, 2035, 2439, 2480, 3234
BseLI CCNNNNNNNGG 9 cut(s) 500, 605, 672, 2327, 2551, 2623, 3437, 3890, 3965
BseMI GCAATG 2 cut(s) 654, 2126
BseMII CTCAG 6 cut(s) 776, 1701, 1958, 2792, 3788, 3876
BseNI ACTGG 6 cut(s) 1335, 1392, 2257, 2556, 2637, 3980
BseRI GAGGAG 7 cut(s) 48, 386, 773, 2834, 3170, 3450, 3902
BseSI GKGCMC 1 cut(s) 1684
BseXI GCAGC 6 cut(s) 137, 138, 855, 1093, 1438, 2844
BseYI CCCAGC 2 cut(s) 996, 1981
BsgI GTGCAG 2 cut(s) 144, 3034
Bsh1236I CGCG 1 cut(s) 161
BshFI GGCC 7 cut(s) 324, 466, 493, 2616, 3225, 3503, 3643
BshNI GGYRCC 3 cut(s) 1749, 1890, 3801
BshVI ATCGAT 1 cut(s) 3066
BsiHKAI GWGCWC 2 cut(s) 2211, 3753
BsiHKCI CYCGRG 2 cut(s) 2481, 3170
BslFI GGGAC 5 cut(s) 648, 2053, 3252, 3419, 3906
BslI CCNNNNNNNGG 9 cut(s) 500, 605, 672, 2327, 2551, 2623, 3437, 3890, 3965
BsmAI GTCTC 7 cut(s) 1036, 1190, 1304, 1452, 2036, 2429, 2772
BsmFI GGGAC 5 cut(s) 648, 2053, 3252, 3419, 3906
BsmI GAATGC 2 cut(s) 26, 1019
BsnI GGCC 7 cut(s) 324, 466, 493, 2616, 3225, 3503, 3643
Bso31I GGTCTC 2 cut(s) 1304, 2429
BsoBI CYCGRG 2 cut(s) 2481, 3170
Bsp119I TTCGAA 1 cut(s) 3622
Bsp1286I GDGCHC 3 cut(s) 1684, 2211, 3753
Bsp1407I TGTACA 1 cut(s) 1806
BspANI GGCC 7 cut(s) 324, 466, 493, 2616, 3225, 3503, 3643
BspCNI CTCAG 6 cut(s) 777, 1702, 1959, 2793, 3789, 3877
BspDI ATCGAT 1 cut(s) 3066
BspFNI CGCG 1 cut(s) 161
BspLI GGNNCC 6 cut(s) 323, 1751, 1892, 3120, 3788, 3803
BspMAI CTGCAG 5 cut(s) 156, 873, 1456, 1864, 3956
BspMI ACCTGC 1 cut(s) 614
BspOI GCTAGC 2 cut(s) 3233, 3875
BspPI GGATC 4 cut(s) 945, 1270, 3113, 3126
BspT104I TTCGAA 1 cut(s) 3622
BspT107I GGYRCC 3 cut(s) 1749, 1890, 3801
BspTNI GGTCTC 2 cut(s) 1304, 2429
BsrBI CCGCTC 2 cut(s) 146, 3010
BsrDI GCAATG 2 cut(s) 654, 2126
BsrGI TGTACA 1 cut(s) 1806
BsrI ACTGG 6 cut(s) 1335, 1392, 2257, 2556, 2637, 3980
BssECI CCNNGG 7 cut(s) 19, 1101, 1117, 2035, 2439, 2480, 3234
BssNI GRCGYC 1 cut(s) 96
BssSI CACGAG 1 cut(s) 921
BssT1I CCWWGG 3 cut(s) 1101, 1117, 2035
Bst2BI CACGAG 1 cut(s) 921
Bst2UI CCWGG 7 cut(s) 600, 1372, 1533, 2441, 3235, 3650, 3960
Bst6I CTCTTC 2 cut(s) 1207, 3852
BstACI GRCGYC 1 cut(s) 96
BstAPI GCANNNNNTGC 1 cut(s) 1859
BstAUI TGTACA 1 cut(s) 1806
BstBI TTCGAA 1 cut(s) 3622
BstDEI CTNAG 9 cut(s) 339, 785, 939, 1710, 1967, 2801, 3683, 3797, 3885
BstEII GGTNACC 1 cut(s) 580
BstENI CCTNNNNNAGG 1 cut(s) 3963
BstFNI CGCG 1 cut(s) 161
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 2 cut(s) 130, 276
BstMAI GTCTC 7 cut(s) 1036, 1190, 1304, 1452, 2036, 2429, 2772
BstMWI GCNNNNNNNGC 9 cut(s) 93, 134, 143, 271, 466, 1859, 1992, 2093, 2206
BstNI CCWGG 7 cut(s) 600, 1372, 1533, 2441, 3235, 3650, 3960
BstNSI RCATGY 7 cut(s) 299, 846, 982, 1019, 1465, 2285, 3520
BstPAI GACNNNNGTC 1 cut(s) 2335
BstPI GGTNACC 1 cut(s) 580
BstSCI CCNGG 7 cut(s) 598, 1370, 1531, 2439, 3233, 3648, 3958
BstSFI CTRYAG 6 cut(s) 152, 869, 1452, 1860, 2373, 3952
BstSLI GKGCMC 1 cut(s) 1684
BstUI CGCG 1 cut(s) 161
BstV1I GCAGC 6 cut(s) 137, 138, 855, 1093, 1438, 2844
BstV2I GAAGAC 8 cut(s) 7, 625, 2331, 2642, 2921, 3519, 3527, 3847
BstX2I RGATCY 2 cut(s) 950, 3118
BstYI RGATCY 2 cut(s) 950, 3118
Bsu15I ATCGAT 1 cut(s) 3066
Bsu36I CCTNAGG 1 cut(s) 3885
BsuRI GGCC 7 cut(s) 324, 466, 493, 2616, 3225, 3503, 3643
BsuTUI ATCGAT 1 cut(s) 3066
BtgZI GCGATG 1 cut(s) 2738
BtsI GCAGTG 1 cut(s) 3935
BtsIMutI CAGTG 5 cut(s) 1964, 2211, 2382, 3529, 3935
BveI ACCTGC 1 cut(s) 614
CaiI CAGNNNCTG 1 cut(s) 40
CfoI GCGC 2 cut(s) 130, 276
Cfr13I GGNCC 5 cut(s) 322, 491, 1389, 2018, 3502
ClaI ATCGAT 1 cut(s) 3066
CseI GACGC 3 cut(s) 85, 167, 2287
Csp6I GTAC 8 cut(s) 317, 1672, 1750, 1807, 1910, 1971, 2687, 3175
CspCI CAANNNNNGTGG 2 cut(s) 281, 316
CviQI GTAC 8 cut(s) 317, 1672, 1750, 1807, 1910, 1971, 2687, 3175
DdeI CTNAG 9 cut(s) 339, 785, 939, 1710, 1967, 2801, 3683, 3797, 3885
EaeI YGGCCR 1 cut(s) 2614
Eam1104I CTCTTC 2 cut(s) 1207, 3852
EarI CTCTTC 2 cut(s) 1207, 3852
EciI GGCGGA 1 cut(s) 903
Eco130I CCWWGG 3 cut(s) 1101, 1117, 2035
Eco31I GGTCTC 2 cut(s) 1304, 2429
Eco32I GATATC 2 cut(s) 399, 3697
Eco47I GGWCC 2 cut(s) 1389, 2018
Eco47III AGCGCT 1 cut(s) 129
Eco57I CTGAAG 6 cut(s) 17, 1757, 2933, 3521, 3531, 3841
Eco81I CCTNAGG 1 cut(s) 3885
Eco88I CYCGRG 2 cut(s) 2481, 3170
Eco91I GGTNACC 1 cut(s) 580
EcoNI CCTNNNNNAGG 1 cut(s) 3963
EcoO109I RGGNCCY 3 cut(s) 322, 2018, 3502
EcoO65I GGTNACC 1 cut(s) 580
EcoRI GAATTC 1 cut(s) 358
EcoRII CCWGG 7 cut(s) 598, 1370, 1531, 2439, 3233, 3648, 3958
EcoRV GATATC 2 cut(s) 399, 3697
EcoT14I CCWWGG 3 cut(s) 1101, 1117, 2035
EcoT22I ATGCAT 2 cut(s) 1021, 1422
ErhI CCWWGG 3 cut(s) 1101, 1117, 2035
FalI AAGNNNNNCTT 2 cut(s) 2765, 2797
FaqI GGGAC 5 cut(s) 648, 2053, 3252, 3419, 3906
FauNDI CATATG 1 cut(s) 4004
FbaI TGATCA 1 cut(s) 550
FblI GTMKAC 2 cut(s) 99, 2372
GlaI GCGC 2 cut(s) 129, 275
GsaI CCCAGC 2 cut(s) 1000, 1985
GsuI CTGGAG 3 cut(s) 2462, 2573, 3442
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 7 cut(s) 324, 466, 493, 2616, 3225, 3503, 3643
HgaI GACGC 3 cut(s) 85, 167, 2287
HhaI GCGC 2 cut(s) 130, 276
Hin1I GRCGYC 1 cut(s) 96
Hin6I GCGC 2 cut(s) 128, 274
HinP1I GCGC 2 cut(s) 128, 274
HincII GTYRAC 3 cut(s) 54, 100, 3304
HindII GTYRAC 3 cut(s) 54, 100, 3304
HindIII AAGCTT 3 cut(s) 1051, 2594, 2789
HpaI GTTAAC 2 cut(s) 54, 3304
HphI GGTGA 5 cut(s) 1445, 3125, 3260, 3370, 4028
Hpy166II GTNNAC 6 cut(s) 54, 100, 1325, 2373, 3304, 3935
Hpy8I GTNNAC 6 cut(s) 54, 100, 1325, 2373, 3304, 3935
Hpy99I CGWCG 1 cut(s) 101
HpyCH4IV ACGT 4 cut(s) 279, 2647, 2863, 3300
HpyF10VI GCNNNNNNNGC 9 cut(s) 93, 134, 143, 271, 466, 1859, 1992, 2093, 2206
HpyF3I CTNAG 9 cut(s) 339, 785, 939, 1710, 1967, 2801, 3683, 3797, 3885
HpySE526I ACGT 4 cut(s) 279, 2647, 2863, 3300
Hsp92I GRCGYC 1 cut(s) 96
HspAI GCGC 2 cut(s) 128, 274
KpnI GGTACC 1 cut(s) 1753
Ksp22I TGATCA 1 cut(s) 550
KspAI GTTAAC 2 cut(s) 54, 3304
LmnI GCTCC 8 cut(s) 143, 890, 1360, 2728, 2821, 3577, 3756, 4025
Lsp1109I GCAGC 6 cut(s) 137, 138, 855, 1093, 1438, 2844
LweI GCATC 8 cut(s) 634, 1750, 2074, 2531, 2600, 3170, 3884, 4002
MaeII ACGT 4 cut(s) 279, 2647, 2863, 3300
MbiI CCGCTC 2 cut(s) 146, 3010
MfeI CAATTG 3 cut(s) 618, 2618, 2631
MflI RGATCY 2 cut(s) 950, 3118
MhlI GDGCHC 3 cut(s) 1684, 2211, 3753
MlyI GAGTC 6 cut(s) 790, 1122, 1192, 2748, 3666, 3888
MmeI TCCRAC 2 cut(s) 2271, 3672
Mph1103I ATGCAT 2 cut(s) 1021, 1422
MroXI GAANNNNTTC 2 cut(s) 3709, 3846
MslI CAYNNNNRTG 5 cut(s) 1318, 2694, 2833, 2923, 2937
MspA1I CMGCKG 1 cut(s) 3861
MspR9I CCNGG 7 cut(s) 600, 1372, 1533, 2441, 3235, 3650, 3960
MunI CAATTG 3 cut(s) 618, 2618, 2631
Mva1269I GAATGC 2 cut(s) 26, 1019
MvaI CCWGG 7 cut(s) 600, 1372, 1533, 2441, 3235, 3650, 3960
MvnI CGCG 1 cut(s) 161
MwoI GCNNNNNNNGC 9 cut(s) 93, 134, 143, 271, 466, 1859, 1992, 2093, 2206
NdeI CATATG 1 cut(s) 4004
NheI GCTAGC 2 cut(s) 3229, 3871
NlaIV GGNNCC 6 cut(s) 323, 1751, 1892, 3120, 3788, 3803
NmeAIII GCCGAG 1 cut(s) 495
NsiI ATGCAT 2 cut(s) 1021, 1422
NspI RCATGY 7 cut(s) 299, 846, 982, 1019, 1465, 2285, 3520
NspV TTCGAA 1 cut(s) 3622
PaeR7I CTCGAG 1 cut(s) 3170
PciI ACATGT 2 cut(s) 842, 1461
PctI GAATGC 2 cut(s) 26, 1019
PdmI GAANNNNTTC 2 cut(s) 3709, 3846
PflMI CCANNNNNTGG 3 cut(s) 605, 672, 2623
PfoI TCCNGGA 1 cut(s) 3648
PleI GAGTC 6 cut(s) 789, 1121, 1191, 2748, 3666, 3888
PpsI GAGTC 6 cut(s) 789, 1121, 1191, 2748, 3666, 3888
PpuMI RGGWCCY 1 cut(s) 2018
PscI ACATGT 2 cut(s) 842, 1461
PshAI GACNNNNGTC 1 cut(s) 2335
PsiI TTATAA 2 cut(s) 2643, 3026
Psp5II RGGWCCY 1 cut(s) 2018
Psp6I CCWGG 7 cut(s) 598, 1370, 1531, 2439, 3233, 3648, 3958
PspEI GGTNACC 1 cut(s) 580
PspFI CCCAGC 2 cut(s) 996, 1981
PspGI CCWGG 7 cut(s) 598, 1370, 1531, 2439, 3233, 3648, 3958
PspN4I GGNNCC 6 cut(s) 323, 1751, 1892, 3120, 3788, 3803
PspPI GGNCC 5 cut(s) 322, 491, 1389, 2018, 3502
PspPPI RGGWCCY 1 cut(s) 2018
PspXI VCTCGAGB 1 cut(s) 3170
PstI CTGCAG 5 cut(s) 156, 873, 1456, 1864, 3956
PstNI CAGNNNCTG 1 cut(s) 40
PsuI RGATCY 2 cut(s) 950, 3118
PvuII CAGCTG 1 cut(s) 3861
RsaI GTAC 8 cut(s) 318, 1673, 1751, 1808, 1911, 1972, 2688, 3176
RsaNI GTAC 8 cut(s) 317, 1672, 1750, 1807, 1910, 1971, 2687, 3175
RseI CAYNNNNRTG 5 cut(s) 1318, 2694, 2833, 2923, 2937
SalI GTCGAC 1 cut(s) 98
Sau96I GGNCC 5 cut(s) 322, 491, 1389, 2018, 3502
ScaI AGTACT 3 cut(s) 1673, 1972, 3176
SchI GAGTC 6 cut(s) 790, 1122, 1192, 2748, 3666, 3888
ScrFI CCNGG 7 cut(s) 600, 1372, 1533, 2441, 3235, 3650, 3960
SduI GDGCHC 3 cut(s) 1684, 2211, 3753
SfaNI GCATC 8 cut(s) 634, 1750, 2074, 2531, 2600, 3170, 3884, 4002
SfcI CTRYAG 6 cut(s) 152, 869, 1452, 1860, 2373, 3952
Sfr274I CTCGAG 1 cut(s) 3170
SfuI TTCGAA 1 cut(s) 3622
SinI GGWCC 2 cut(s) 1389, 2018
SlaI CTCGAG 1 cut(s) 3170
SmiMI CAYNNNNRTG 5 cut(s) 1318, 2694, 2833, 2923, 2937
SmlI CTYRAG 8 cut(s) 110, 1675, 2053, 2393, 3170, 3178, 3614, 3657
SmoI CTYRAG 8 cut(s) 110, 1675, 2053, 2393, 3170, 3178, 3614, 3657
SpeI ACTAGT 2 cut(s) 2810, 3718
SspI AATATT 1 cut(s) 1164
StyD4I CCNGG 7 cut(s) 598, 1370, 1531, 2439, 3233, 3648, 3958
StyI CCWWGG 3 cut(s) 1101, 1117, 2035
TaiI ACGT 4 cut(s) 282, 2650, 2866, 3303
TatI WGTACW 4 cut(s) 1671, 1806, 1970, 3174
TauI GCSGC 4 cut(s) 274, 469, 1891, 2616
TscAI CASTG 5 cut(s) 1971, 2218, 2382, 3536, 3942
TseI GCWGC 6 cut(s) 125, 151, 868, 1106, 1451, 2857
TspRI CASTG 5 cut(s) 1971, 2218, 2382, 3536, 3942
Van91I CCANNNNNTGG 3 cut(s) 605, 672, 2623
VpaK11BI GGWCC 2 cut(s) 1389, 2018
XagI CCTNNNNNAGG 1 cut(s) 3963
XapI RAATTY 7 cut(s) 358, 771, 1207, 1256, 1556, 1565, 2664
XbaI TCTAGA 2 cut(s) 332, 3580
XceI RCATGY 7 cut(s) 299, 846, 982, 1019, 1465, 2285, 3520
XhoI CTCGAG 1 cut(s) 3170
XmiI GTMKAC 2 cut(s) 99, 2372
XmnI GAANNNNTTC 2 cut(s) 3709, 3846
ZrmI AGTACT 3 cut(s) 1673, 1972, 3176
Zsp2I ATGCAT 2 cut(s) 1021, 1422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.