Rmu_sc0002745.1_g000006

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002745.1
OG0000070
Physical Location & Seq
Forward (+)
28045 .. 30666
2622 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002745.1_g000006.1.cds

Sequence Viewer

Length: 2622 bp
atgcatgacctcgttggtgacttggcacgatgggctgctgcagacacattttgcagattggaggataaactgcatggtagatgttctgcaaagactcgccatctggcttacatttctagtaagttcgatggggtaaaaagatttgtgacattttcggaagccaaacgcttgcggactttcctaccacttcctgtttccggtggttatgagaattatctaactcgttatgctacttctgatttattgccacaattgaagtacttgcgggtgctctccttcaatggctataaactgaccgaggtgccaaattccgtaggtaagttgagacatctacggtaccttgatctttctcacacattaataacgtgtttgcctgagtcaacatgcatgctttacaacttacagacgttaatattagagaattgttcaaaattgaagacactaccttcaaacatgagcaatttatctaatttacgtcatctcaacaattcaaacatgccttcattggaagaaatgcctcccaaagtaagtcggttgactcatctgcgaactttgcctaattttgtggtggggaaaggtagtgcatcagggattggagagatacagtcattgcatcttcgtgggaccttgagtgttggaagattggaaaatgtgattgatgttggggatgcgaagacggctcaaataatgaacaaggaagggctggaaatcttgcaattggaatggagcggcacaagcgagaaggagttggaggtgctgtgcagtttagaacctcataaaaagcttaaggagctgaccatcaagggctacaatggtttcgaattttcaaaatggattggacatccttcattctctgatatgaagtctgtgaaattggagaattgtaaaaactgtcgattcttacctcctcttggccaattaccttttctgaaaaaacttcagatacaaggactggccaatgttgaaagtgtgggtgctgagttttatggagagtgcagcttgccttttccagtgttggaagacctgacgttcgaggatatgcaaaattggaaggagtggcttccttgcaaaagagatgaagaaattcgagttttcccacgcatggaaaatcttttaatcttttgttgccccaaattgaaaggttggttgcccaagaacatggattcattatcacagatgtacattgctgaatgtgaagagttagtggtttcaatagccaattataaacatcttcgtatgctgatcatccgcggttgtaaaagggtggtgcaccgaagtggagttaagtttgagttactggaggccatggggctttataatatttcagagttcagactcgaaatagacgggttcatcagaggattgtcaaagcttcaaggattgtggattactggttgtgaagagttgacatgtttatgggagaatgaggataggtggctgcagcgtcgaatttccaatattattggaggcaacatatctgactccccctctcactttattcaagagctaagagcactcggtgtacttgagttaactggatgctcaaatctaatttcttttccagaagctagttggccaccttgtcttgaatttgtaaagatggtgtcgtgtaattctttgacgcatttcgtaaggtatcaagtaccgccaagcataagaagaatagagatagaagaatgtcaaaatttgaaactactagtgaaggatgctggggagctagagggttgtatggagtggttggagataaaagagtgtgcatctttgacatccttatcaggcaaaggaggccgactacccagaaccctcaaatatctccatttaagtgcctgtgaacaattggagtcaataatcaaaacattccacgaggacacttgtcttgaagctcttacgatagtgaagtgtgcaaatctcaaatccttaccagaggggctatgccatctctccgctcttcgcatgttatggatagaggaatgtggaagtctcgtttcctttccaagaggagggttgccatccaacctggcatacttgtctatctccatttgtgctcaattggaagccctccccagaggcatggacaacctcagctctcttcagactttggtgttgagttactgtggaggtttggcatccattctagaagagggtttccctcccaacctaattaatctgaaaattggccatcccgaaagctgcaattctctctcagagtgggggcttcaccacttggacagactcacctctcttgaaaccttttctatcttcagtggagatccagatctggtgtcctttccgccaaaggaggtgctgctccctaaatctctcattaaactcgacattgcaggcttcccaaatctgaagcgcctgtcatcatcttttcaatcactcacctctcttgaatcccttgacattcttggatgcccaaagctagcatctattattccagaacaagaggatcatctgcctctttcacttacacagcttcgcatccgtgagggctgtccgctgctcacaaagaaatacaaaccaggtaaaggacgacactggcccaaacaaattgcccacatcccttacgtttatgttggagactgcaatgacgaagccgaagccgaccgccgggcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

873

Amino Acids

98.41

Weight (kDa)

6.9

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1224, 1317
Acc65I GGTACC 1 cut(s) 336
AccB1I GGYRCC 2 cut(s) 301, 336
AccBSI CCGCTC 2 cut(s) 729, 1946
AccII CGCG 1 cut(s) 1251
AclWI GGATC 2 cut(s) 2264, 2460
AcoI YGGCCR 4 cut(s) 913, 954, 1574, 2176
AcsI RAATTY 6 cut(s) 307, 821, 1083, 1449, 1589, 1684
AcuI CTGAAG 4 cut(s) 923, 2075, 2245, 2375
AdeI CACNNNGTG 1 cut(s) 1520
AfaI GTAC 5 cut(s) 260, 338, 1181, 1524, 1644
AfiI CCNNNNNNNGG 6 cut(s) 197, 911, 1102, 2000, 2531, 2613
AflII CTTAAG 1 cut(s) 785
AflIII ACRYGT 2 cut(s) 365, 1409
AhlI ACTAGT 1 cut(s) 1696
AjnI CCWGG 2 cut(s) 2016, 2524
AjuI GAANNNNNNNTTGG 2 cut(s) 909, 941
AleI CACNNNNGTG 1 cut(s) 1275
Alw21I GWGCWC 4 cut(s) 273, 1272, 1516, 2047
Alw26I GTCTC 3 cut(s) 319, 1985, 2577
Alw44I GTGCAC 1 cut(s) 1268
AlwI GGATC 2 cut(s) 2264, 2460
AoxI GGCC 7 cut(s) 913, 954, 1302, 1574, 1786, 2176, 2543
ApaLI GTGCAC 1 cut(s) 1268
ApeKI GCWGC 8 cut(s) 35, 38, 996, 1438, 1441, 2190, 2305, 2503
ApoI RAATTY 6 cut(s) 307, 821, 1083, 1449, 1589, 1684
ArsI GACNNNNNNTTYG 2 cut(s) 1013, 1045
AseI ATTAAT 2 cut(s) 359, 2163
Asp700I GAANNNNTTC 1 cut(s) 1083
Asp718I GGTACC 1 cut(s) 336
AspLEI GCGC 1 cut(s) 2361
AspS9I GGNCC 2 cut(s) 624, 2544
AsuC2I CCSGG 1 cut(s) 2615
AsuHPI GGTGA 4 cut(s) 29, 2210, 2227, 2377
AsuII TTCGAA 1 cut(s) 819
AsuNHI GCTAGC 1 cut(s) 2425
AvaII GGWCC 1 cut(s) 624
BaeGI GKGCMC 1 cut(s) 1272
BalI TGGCCA 4 cut(s) 915, 956, 1576, 2178
BanI GGYRCC 2 cut(s) 301, 336
BauI CACGAG 1 cut(s) 1862
BbsI GAAGAC 3 cut(s) 443, 680, 1026
Bbv12I GWGCWC 4 cut(s) 273, 1272, 1516, 2047
BbvCI CCTCAGC 1 cut(s) 2081
BbvI GCAGC 8 cut(s) 22, 25, 1008, 1425, 1453, 2177, 2292, 2490
BccI CCATC 8 cut(s) 24, 108, 122, 806, 1594, 1944, 2017, 2187
BceAI ACGGC 1 cut(s) 693
BciT130I CCWGG 2 cut(s) 2018, 2526
BclI TGATCA 1 cut(s) 1242
BcnI CCSGG 1 cut(s) 2615
BcoDI GTCTC 3 cut(s) 319, 1985, 2577
BcuI ACTAGT 1 cut(s) 1696
BfaI CTAG 6 cut(s) 117, 1569, 1697, 1718, 2135, 2426
BfmI CTRYAG 2 cut(s) 39, 1439
BfoI RGCGCY 1 cut(s) 2362
BfrI CTTAAG 1 cut(s) 785
BglII AGATCT 1 cut(s) 2275
BisI GCNGC 9 cut(s) 36, 39, 730, 997, 1439, 1442, 2191, 2306, 2504
BlsI GCNGC 9 cut(s) 37, 40, 731, 998, 1440, 1443, 2192, 2307, 2505
BmcAI AGTACT 1 cut(s) 260
Bme1390I CCNGG 3 cut(s) 2018, 2526, 2615
Bme18I GGWCC 1 cut(s) 624
BmgT120I GGNCC 2 cut(s) 624, 2544
BmiI GGNNCC 3 cut(s) 303, 338, 625
BmrFI CCNGG 3 cut(s) 2018, 2526, 2615
BmtI GCTAGC 1 cut(s) 2429
BoxI GACNNNNGTC 1 cut(s) 1872
BpiI GAAGAC 3 cut(s) 443, 680, 1026
BpmI CTGGAG 1 cut(s) 1319
Bpu10I CCTNAGC 1 cut(s) 2081
Bpu14I TTCGAA 1 cut(s) 819
BpuEI CTTGAG 2 cut(s) 649, 1547
BpuMI CCSGG 1 cut(s) 2615
BsaBI GATNNNNATC 1 cut(s) 2274
BsaJI CCNNGG 3 cut(s) 297, 1249, 1305
BsaWI WCCGGW 1 cut(s) 197
BsaXI ACNNNNNCTCC 2 cut(s) 743, 773
Bsc4I CCNNNNNNNGG 6 cut(s) 197, 911, 1102, 2000, 2531, 2613
Bse1I ACTGG 6 cut(s) 957, 1010, 1302, 1396, 1540, 2546
Bse3DI GCAATG 4 cut(s) 608, 1182, 2334, 2596
Bse8I GATNNNNATC 1 cut(s) 2274
BseBI CCWGG 2 cut(s) 2018, 2526
BseDI CCNNGG 3 cut(s) 297, 1249, 1305
BseJI GATNNNNATC 1 cut(s) 2274
BseLI CCNNNNNNNGG 6 cut(s) 197, 911, 1102, 2000, 2531, 2613
BseMI GCAATG 4 cut(s) 608, 1182, 2334, 2596
BseMII CTCAG 4 cut(s) 366, 969, 2095, 2217
BseNI ACTGG 6 cut(s) 957, 1010, 1302, 1396, 1540, 2546
BseRI GAGGAG 2 cut(s) 897, 2013
BseSI GKGCMC 1 cut(s) 1272
BseXI GCAGC 8 cut(s) 22, 25, 1008, 1425, 1453, 2177, 2292, 2490
BseYI CCCAGC 1 cut(s) 1709
BsgI GTGCAG 2 cut(s) 781, 1015
Bsh1236I CGCG 1 cut(s) 1251
Bsh1285I CGRYCG 1 cut(s) 2611
BshFI GGCC 7 cut(s) 915, 956, 1304, 1576, 1788, 2178, 2545
BshNI GGYRCC 2 cut(s) 301, 336
BsiEI CGRYCG 1 cut(s) 2611
BsiHKAI GWGCWC 4 cut(s) 273, 1272, 1516, 2047
BsiSI CCGG 2 cut(s) 198, 2614
BslFI GGGAC 1 cut(s) 637
BslI CCNNNNNNNGG 6 cut(s) 197, 911, 1102, 2000, 2531, 2613
BsmAI GTCTC 3 cut(s) 319, 1985, 2577
BsmFI GGGAC 1 cut(s) 637
BsnI GGCC 7 cut(s) 915, 956, 1304, 1576, 1788, 2178, 2545
Bsp119I TTCGAA 1 cut(s) 819
Bsp1286I GDGCHC 4 cut(s) 273, 1272, 1516, 2047
Bsp1407I TGTACA 1 cut(s) 1179
Bsp143I GATC 5 cut(s) 343, 1242, 2269, 2275, 2452
Bsp19I CCATGG 1 cut(s) 1305
BspANI GGCC 7 cut(s) 915, 956, 1304, 1576, 1788, 2178, 2545
BspCNI CTCAG 4 cut(s) 367, 970, 2094, 2216
BspFNI CGCG 1 cut(s) 1251
BspLI GGNNCC 3 cut(s) 303, 338, 625
BspMAI CTGCAG 2 cut(s) 43, 1443
BspOI GCTAGC 1 cut(s) 2429
BspPI GGATC 2 cut(s) 2264, 2460
BspQI GCTCTTC 1 cut(s) 1953
BspT104I TTCGAA 1 cut(s) 819
BspT107I GGYRCC 2 cut(s) 301, 336
BspTI CTTAAG 1 cut(s) 785
BsrBI CCGCTC 2 cut(s) 729, 1946
BsrDI GCAATG 4 cut(s) 608, 1182, 2334, 2596
BsrGI TGTACA 1 cut(s) 1179
BsrI ACTGG 6 cut(s) 957, 1010, 1302, 1396, 1540, 2546
BssECI CCNNGG 3 cut(s) 297, 1249, 1305
BssMI GATC 5 cut(s) 343, 1242, 2269, 2275, 2452
BssSI CACGAG 1 cut(s) 1862
BssT1I CCWWGG 1 cut(s) 1305
Bst2BI CACGAG 1 cut(s) 1862
Bst2UI CCWGG 2 cut(s) 2018, 2526
Bst4CI ACNGT 4 cut(s) 336, 606, 893, 2114
Bst6I CTCTTC 5 cut(s) 1191, 1395, 1953, 2094, 2133
BstAFI CTTAAG 1 cut(s) 785
BstAUI TGTACA 1 cut(s) 1179
BstBI TTCGAA 1 cut(s) 819
BstC8I GCNNGC 5 cut(s) 170, 389, 1001, 2341, 2427
BstDEI CTNAG 5 cut(s) 375, 978, 1508, 2081, 2203
BstDSI CCRYGG 2 cut(s) 1249, 1305
BstFNI CGCG 1 cut(s) 1251
BstH2I RGCGCY 1 cut(s) 2362
BstHHI GCGC 1 cut(s) 2361
BstKTI GATC 5 cut(s) 346, 1245, 2272, 2278, 2455
BstMAI GTCTC 3 cut(s) 319, 1985, 2577
BstMBI GATC 5 cut(s) 343, 1242, 2269, 2275, 2452
BstMCI CGRYCG 1 cut(s) 2611
BstMWI GCNNNNNNNGC 6 cut(s) 32, 553, 677, 735, 790, 1785
BstNI CCWGG 2 cut(s) 2018, 2526
BstNSI RCATGY 5 cut(s) 387, 391, 499, 1413, 1957
BstPAI GACNNNNGTC 1 cut(s) 1872
BstSCI CCNGG 3 cut(s) 2016, 2524, 2613
BstSFI CTRYAG 2 cut(s) 39, 1439
BstSLI GKGCMC 1 cut(s) 1272
BstUI CGCG 1 cut(s) 1251
BstV1I GCAGC 8 cut(s) 22, 25, 1008, 1425, 1453, 2177, 2292, 2490
BstV2I GAAGAC 3 cut(s) 443, 680, 1026
BstX2I RGATCY 2 cut(s) 2269, 2275
BstXI CCANNNNNNTGG 2 cut(s) 1159, 2071
BstYI RGATCY 2 cut(s) 2269, 2275
BsuRI GGCC 7 cut(s) 915, 956, 1304, 1576, 1788, 2178, 2545
BtgI CCRYGG 2 cut(s) 1249, 1305
BtsIMutI CAGTG 3 cut(s) 1017, 2269, 2539
Cac8I GCNNGC 5 cut(s) 170, 389, 1001, 2341, 2427
CfoI GCGC 1 cut(s) 2361
Cfr13I GGNCC 2 cut(s) 624, 2544
Cfr42I CCGCGG 1 cut(s) 1252
CseI GACGC 2 cut(s) 1433, 1630
CsiI ACCWGGT 1 cut(s) 2524
Csp6I GTAC 5 cut(s) 259, 337, 1180, 1523, 1643
CspCI CAANNNNNGTGG 2 cut(s) 1364, 1399
CviQI GTAC 5 cut(s) 259, 337, 1180, 1523, 1643
DdeI CTNAG 5 cut(s) 375, 978, 1508, 2081, 2203
DpnI GATC 5 cut(s) 345, 1244, 2271, 2277, 2454
DpnII GATC 5 cut(s) 343, 1242, 2269, 2275, 2452
DraIII CACNNNGTG 1 cut(s) 1520
EaeI YGGCCR 4 cut(s) 913, 954, 1574, 2176
Eam1104I CTCTTC 5 cut(s) 1191, 1395, 1953, 2094, 2133
EarI CTCTTC 5 cut(s) 1191, 1395, 1953, 2094, 2133
EciI GGCGGA 1 cut(s) 2280
Eco130I CCWWGG 1 cut(s) 1305
Eco47I GGWCC 1 cut(s) 624
Eco57I CTGAAG 4 cut(s) 923, 2075, 2245, 2375
EcoO109I RGGNCCY 1 cut(s) 624
EcoRII CCWGG 2 cut(s) 2016, 2524
EcoT14I CCWWGG 1 cut(s) 1305
EcoT22I ATGCAT 2 cut(s) 6, 389
ErhI CCWWGG 1 cut(s) 1305
FaqI GGGAC 1 cut(s) 637
FauI CCCGC 1 cut(s) 258
FbaI TGATCA 1 cut(s) 1242
Fnu4HI GCNGC 9 cut(s) 36, 39, 730, 997, 1439, 1442, 2191, 2306, 2504
Fsp4HI GCNGC 9 cut(s) 36, 39, 730, 997, 1439, 1442, 2191, 2306, 2504
FspBI CTAG 6 cut(s) 117, 1569, 1697, 1718, 2135, 2426
GlaI GCGC 1 cut(s) 2360
GluI GCNGC 9 cut(s) 36, 39, 730, 997, 1439, 1442, 2191, 2306, 2504
GsaI CCCAGC 1 cut(s) 1713
GsuI CTGGAG 1 cut(s) 1319
HaeII RGCGCY 1 cut(s) 2362
HaeIII GGCC 7 cut(s) 915, 956, 1304, 1576, 1788, 2178, 2545
HapII CCGG 2 cut(s) 198, 2614
HgaI GACGC 2 cut(s) 1433, 1630
HhaI GCGC 1 cut(s) 2361
Hin6I GCGC 1 cut(s) 2359
HinP1I GCGC 1 cut(s) 2359
HincII GTYRAC 4 cut(s) 381, 537, 1407, 1533
HindII GTYRAC 4 cut(s) 381, 537, 1407, 1533
HindIII AAGCTT 2 cut(s) 782, 1370
HpaI GTTAAC 1 cut(s) 1533
HpaII CCGG 2 cut(s) 198, 2614
HphI GGTGA 4 cut(s) 29, 2210, 2227, 2377
Hpy166II GTNNAC 7 cut(s) 381, 537, 1270, 1407, 1523, 1533, 1832
Hpy8I GTNNAC 7 cut(s) 381, 537, 1270, 1407, 1523, 1533, 1832
Hpy99I CGWCG 1 cut(s) 1449
HpyAV CCTTC 8 cut(s) 286, 456, 510, 692, 736, 855, 1045, 1696
HpyCH4III ACNGT 4 cut(s) 336, 606, 893, 2114
HpyCH4IV ACGT 5 cut(s) 365, 407, 475, 1028, 2571
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 553, 677, 735, 790, 1785
HpyF3I CTNAG 5 cut(s) 375, 978, 1508, 2081, 2203
HpySE526I ACGT 5 cut(s) 365, 407, 475, 1028, 2571
HspAI GCGC 1 cut(s) 2359
KpnI GGTACC 1 cut(s) 340
Ksp22I TGATCA 1 cut(s) 1242
KspAI GTTAAC 1 cut(s) 1533
KspI CCGCGG 1 cut(s) 1252
Kzo9I GATC 5 cut(s) 343, 1242, 2269, 2275, 2452
LguI GCTCTTC 1 cut(s) 1953
LmnI GCTCC 4 cut(s) 726, 790, 1714, 2313
Lsp1109I GCAGC 8 cut(s) 22, 25, 1008, 1425, 1453, 2177, 2292, 2490
MabI ACCWGGT 1 cut(s) 2524
MaeI CTAG 6 cut(s) 117, 1569, 1697, 1718, 2135, 2426
MaeII ACGT 5 cut(s) 365, 407, 475, 1028, 2571
MaeIII GTNAC 4 cut(s) 17, 145, 1293, 2108
MalI GATC 5 cut(s) 345, 1244, 2271, 2277, 2454
MbiI CCGCTC 2 cut(s) 729, 1946
MboI GATC 5 cut(s) 343, 1242, 2269, 2275, 2452
MfeI CAATTG 4 cut(s) 251, 716, 1835, 2048
MflI RGATCY 2 cut(s) 2269, 2275
MhlI GDGCHC 4 cut(s) 273, 1272, 1516, 2047
MlsI TGGCCA 4 cut(s) 915, 956, 1576, 2178
MluNI TGGCCA 4 cut(s) 915, 956, 1576, 2178
MlyI GAGTC 7 cut(s) 88, 386, 532, 1329, 1475, 1850, 2226
MmeI TCCRAC 6 cut(s) 616, 729, 996, 1719, 2037, 2560
Mox20I TGGCCA 4 cut(s) 915, 956, 1576, 2178
Mph1103I ATGCAT 2 cut(s) 6, 389
MroXI GAANNNNTTC 1 cut(s) 1083
MscI TGGCCA 4 cut(s) 915, 956, 1576, 2178
MseI TTAA 9 cut(s) 359, 410, 786, 1115, 1284, 1532, 1820, 2163, 2325
MslI CAYNNNNRTG 4 cut(s) 618, 1275, 1414, 1821
Msp20I TGGCCA 4 cut(s) 915, 956, 1576, 2178
MspA1I CMGCKG 2 cut(s) 1251, 2503
MspCI CTTAAG 1 cut(s) 785
MspI CCGG 2 cut(s) 198, 2614
MspR9I CCNGG 3 cut(s) 2018, 2526, 2615
MunI CAATTG 4 cut(s) 251, 716, 1835, 2048
MvaI CCWGG 2 cut(s) 2018, 2526
MvnI CGCG 1 cut(s) 1251
MwoI GCNNNNNNNGC 6 cut(s) 32, 553, 677, 735, 790, 1785
NciI CCSGG 1 cut(s) 2615
NcoI CCATGG 1 cut(s) 1305
NdeII GATC 5 cut(s) 343, 1242, 2269, 2275, 2452
NheI GCTAGC 1 cut(s) 2425
NlaIV GGNNCC 3 cut(s) 303, 338, 625
NmuCI GTSAC 2 cut(s) 17, 145
NsiI ATGCAT 2 cut(s) 6, 389
NspI RCATGY 5 cut(s) 387, 391, 499, 1413, 1957
NspV TTCGAA 1 cut(s) 819
OliI CACNNNNGTG 1 cut(s) 1275
PaeI GCATGC 1 cut(s) 391
PciI ACATGT 1 cut(s) 1409
PciSI GCTCTTC 1 cut(s) 1953
PdmI GAANNNNTTC 1 cut(s) 1083
PfeI GAWTC 3 cut(s) 897, 1163, 2396
PkrI GCNGC 9 cut(s) 37, 40, 731, 998, 1440, 1443, 2192, 2307, 2505
PleI GAGTC 7 cut(s) 88, 385, 532, 1329, 1475, 1849, 2226
PpsI GAGTC 7 cut(s) 88, 385, 532, 1329, 1475, 1849, 2226
PpuMI RGGWCCY 1 cut(s) 624
PscI ACATGT 1 cut(s) 1409
PshAI GACNNNNGTC 1 cut(s) 1872
PshBI ATTAAT 2 cut(s) 359, 2163
PsiI TTATAA 2 cut(s) 1224, 1317
Psp5II RGGWCCY 1 cut(s) 624
Psp6I CCWGG 2 cut(s) 2016, 2524
PspFI CCCAGC 1 cut(s) 1709
PspGI CCWGG 2 cut(s) 2016, 2524
PspN4I GGNNCC 3 cut(s) 303, 338, 625
PspPI GGNCC 2 cut(s) 624, 2544
PspPPI RGGWCCY 1 cut(s) 624
PstI CTGCAG 2 cut(s) 43, 1443
PsuI RGATCY 2 cut(s) 2269, 2275
RsaI GTAC 5 cut(s) 260, 338, 1181, 1524, 1644
RsaNI GTAC 5 cut(s) 259, 337, 1180, 1523, 1643
RseI CAYNNNNRTG 4 cut(s) 618, 1275, 1414, 1821
SacII CCGCGG 1 cut(s) 1252
SapI GCTCTTC 1 cut(s) 1953
SaqAI TTAA 9 cut(s) 359, 410, 786, 1115, 1284, 1532, 1820, 2163, 2325
SatI GCNGC 9 cut(s) 36, 39, 730, 997, 1439, 1442, 2191, 2306, 2504
Sau3AI GATC 5 cut(s) 343, 1242, 2269, 2275, 2452
Sau96I GGNCC 2 cut(s) 624, 2544
ScaI AGTACT 1 cut(s) 260
SchI GAGTC 7 cut(s) 88, 386, 532, 1329, 1475, 1850, 2226
ScrFI CCNGG 3 cut(s) 2018, 2526, 2615
SduI GDGCHC 4 cut(s) 273, 1272, 1516, 2047
SexAI ACCWGGT 1 cut(s) 2524
SfcI CTRYAG 2 cut(s) 39, 1439
Sfr303I CCGCGG 1 cut(s) 1252
SfuI TTCGAA 1 cut(s) 819
SgrBI CCGCGG 1 cut(s) 1252
SinI GGWCC 1 cut(s) 624
SmiMI CAYNNNNRTG 4 cut(s) 618, 1275, 1414, 1821
SmlI CTYRAG 3 cut(s) 628, 785, 1526
SmoI CTYRAG 3 cut(s) 628, 785, 1526
SpeI ACTAGT 1 cut(s) 1696
SphI GCATGC 1 cut(s) 391
SspI AATATT 3 cut(s) 414, 1321, 1459
SspMI CTAG 6 cut(s) 117, 1569, 1697, 1718, 2135, 2426
StyD4I CCNGG 3 cut(s) 2016, 2524, 2613
StyI CCWWGG 1 cut(s) 1305
TaaI ACNGT 4 cut(s) 336, 606, 893, 2114
TaiI ACGT 5 cut(s) 368, 410, 478, 1031, 2574
TaqI TCGA 8 cut(s) 126, 819, 895, 1032, 1087, 1338, 1447, 2331
TaqII GACCGA 1 cut(s) 311
TatI WGTACW 3 cut(s) 258, 1179, 1522
TauI GCSGC 1 cut(s) 732
TfiI GAWTC 3 cut(s) 897, 1163, 2396
Tru1I TTAA 9 cut(s) 359, 410, 786, 1115, 1284, 1532, 1820, 2163, 2325
Tru9I TTAA 9 cut(s) 359, 410, 786, 1115, 1284, 1532, 1820, 2163, 2325
TscAI CASTG 3 cut(s) 1017, 2269, 2546
TseFI GTSAC 2 cut(s) 17, 145
TseI GCWGC 8 cut(s) 35, 38, 996, 1438, 1441, 2190, 2305, 2503
Tsp45I GTSAC 2 cut(s) 17, 145
TspDTI ATGAA 7 cut(s) 492, 704, 837, 875, 1092, 1155, 1342
TspGWI ACGGA 2 cut(s) 301, 2477
TspRI CASTG 3 cut(s) 1017, 2269, 2546
Vha464I CTTAAG 1 cut(s) 785
VneI GTGCAC 1 cut(s) 1268
VpaK11BI GGWCC 1 cut(s) 624
VspI ATTAAT 2 cut(s) 359, 2163
XapI RAATTY 6 cut(s) 307, 821, 1083, 1449, 1589, 1684
XbaI TCTAGA 1 cut(s) 2134
XceI RCATGY 5 cut(s) 387, 391, 499, 1413, 1957
XcmI CCANNNNNNNNNTGG 1 cut(s) 1569
XmnI GAANNNNTTC 1 cut(s) 1083
XspI CTAG 6 cut(s) 117, 1569, 1697, 1718, 2135, 2426
ZrmI AGTACT 1 cut(s) 260
Zsp2I ATGCAT 2 cut(s) 6, 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.