MD11G1030500.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000070
Physical Location & Seq
Forward (+)
2664259 .. 2669520
5262 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1030500.v1.1.491

Sequence Viewer

Length: 3369 bp
ATGGCGGCAGAGGCTTTTCTTGTGTCATTCCTCCACTTGCTGGTTGACAAGTTGGCGCGTCGCGACGTCTTGAAGTACTTTGGACTCGTAAAAGGCGTCGATAAGAAACTGACGGAATGGATTGCCACCTTGTCTGCAATTGGAGCTGTGCTGATTGACGCGGAGAAAAGGCAACTGGCGGATGAGAGCAACATACTGAAGCTCTGGCTCGATGATCTCAGGGACTTGGCTTTTGATGCGGAAGACGTGTTGGATAAATATCATACTGAAATGTTGAAACGTCAGATACATCATGCTCATTCCAGCGCAACAAGCAAAGCATGGAAATCAATTCCTAATGGTGTTTTCAACTTCAAGATGAACTCAGACATACAGAAGATTACTGAGCGTTTACAAAAGATATCTGTACGAAAAGACCAGCTCGGTTTGAAAATTGATACTGGGACGTTGACTACAAGGGCACGCCAACACATACCGCCTAGTTCTAGCCTACCAGATGGACCTGTGATTGGAAGGGAGGAGGACAAAAGGAAGATTGTTGAGCTGCTGTCAAAACAAGAGCATCGTGCTGTCAATTTCGATGTAGTTGCAATTGTCGGTATGGCTGGAGTCGGAAAGACAACACTTGCTGGACAAGTACTCAAAGATATGGTTGCAACCCAAATGTTTCAACCAGCCGTTTGGGTGTGCGTATCTGACAACTTCAACCTTGAAAGAGTGACAAAGCAAATTCTTCAATCAATCACATCTCGGCAATATACCACAGAAGATTACGAGAAGGTTCAAAATGATCTGCATAAGGAGTTAGCGGGGAAGAAGTTTTTAATTGTTTTAGACGATGTTTGGAAAACGTGTAGCTATGGTGAATGGATGAAGCTGCAGTCCCCTTTTCGTGATGGAGCACAAGGAAGCAAGATAGTTGTGACAACACGTGATACAGATGTTTCAAAAATGATGGGAGCTGCCACGCTGGTTCACAATTTGGTGCCTATGGAAAATGATGTTTGTTTGCAAGTATTTGAGCAGCATGCATTTTTGAATGCTAACAACAATAGACCACCAAACTACGAGTTACTTAAGGAGAAAATTGTTGCAAAGTGTAGGGGATTGCCTTTGGCCGCGAGGACCCTCGGTGGTGTTCTACTTCGTAAAGAAATAGACGAATGGGAAGAAATATTGGACAACAAATTGTGGAGTCTCTCGAATGAGCATGACATACTTCCCGTGTTGAGACTAAGTTACTTTTATCTTCCTTCACATTTGAAAAGATGCTTTGCCTATTGCTCAATACTTCCAAATGACTATGAATTCGGGGAGATGCAAATGATCCTTTTGTGGATGGCCGAGGGTTTGATTCATCCTCGACCAGAAGATAATAAGCAAATTGAAGATTTAGGTGCTGATTATTTTCGGGAGCTTGTATCTAGGTCATTGTTTCAAAAATCAACTGCAAATATTTCAAGATATATGATGCATGACCTCATTGGTGATTTAGCACGATGGGCAGCTGGAGATATTTGTTTTAGATTGGAGGATAAGCAAAATGATGATGGTGTACAACTTAATCCGAAGGCACGCCATTCGTCTTACCTTTTGGGTCATTGTGATGGGGTTAAAAGATTTGAGGCATTTTCTGAAGTGAAATGTTTGCGAACCTTCTTGCCACTAAGAAAGGGTTCTACCTACAATTTTTTAAGTCGTCTGGTTACCTTTGATTTATTGCCAAAATTGCAGTACTTGCGGGTGCTCTCTTTTAATGGCTATCGTATAACTGAGCTGCCAAACTCAATCGGTGATTTGAGACATCTACGGTATCTTGACCTTTCCTACACAGCTATAACTAGTTTGCCTAAATCGATAAGTACTCTTTACAACTTGCAGACATTGATATTGGAAGGCTGTAATGATTTGAAGTCATTGCCTGCAGACATGAGTAATCTAGTTAATTTGCGCCATCTCAACAACTCAAATGTATCTTCGTTGGAAGGAATGCCTCCAAAACTAGGTAGATTGGAGAATCTCCAATCTTTGCCCAATTTTGTGGTGTGTGGTGGAAGTGATGAATCAGGGATAAGAGAGATAGGGCCCCTATCGCATCTCCGAGGGACATTGTGCATCTCAAGATTGGAGAATGTGACTGATTTCGAGGATGCTCAGAAGGCTGACTTAAAATGCAAGGAGTGGCTTGATTCTTTGGTTCTAGAATGGTCTCATTCAAGCGACACAAGAGAAACTGAATCCGCTTTGCTTGACATGTTACAGCCTCATAGAAAGATCAAGGAGCTCACCATCAAGAGTTATGCCGGAAAGGAATTTTCATCATGGGTTGGAGGTCATTTGTTCTCTAATATGGTGCTTGTGCGCTTAGAGGAATGTAACAATTGTTTATCGTTGCCTCCTCTCGGACAATTGCCTCATCTCAGAGAACTTTATATTATTGGAATGAATGCAGTGGAAAGTGTTGGTGCTGAGTTTTGTGGAGAGCGTATGTTGCCTTTTCGGGTATTAGAGACTCTTGAGTTTGTGGAAATGCAGCATTGGAAGGAATGGCTTCCTTTCCAAGCCGATCACGAAGGTGGTGCTTTCCCTTGCCTGAAAACACTCTTAGTAAAAAAATGTTCTAAACTGGAGGGTAAACTGCCAGAGAACCTCAATTCTTTAGCTAAGCTTGAAATTGTTGAATGTGAAGAATTAGTGGTTTCAATTGCCAACTGTAAACAACTTCGTCAGTTAAACATTGACGGTTGTAAAGTGTTGGTGCACACAGCTGCTAAGGTTGAGTTTGAGTACTTAGAGTCCTTGTGCTTTTCAAACATTTCGAAGGTGATGTCTCTGCAAACAGGGGAATTGTTGAAGAAGGGATTAAGCAAGGTTAGAGATTTGAAGATCAGTGGATGTGAGGAGGAGACTGTTGGTTCTTCTTGGTCGTCTTCTACTCATACTTGTCTTGAGTACTTGAGTATCAAAGGATGTGAATCTCTGACGTCGTTATCATTGAGTGGCCAGCTTCCCAGGACACTTAAACACCTTGAGATAGAGAATTGTGAACGACTGGGTTTAATAGCACCGAACGGGTTCTTCAGCGACAACACCAATCACTGCCTTGAATATATTACCATCTGGAATTGCCAAAATCTGAAATCCTTACCGGATGGCTTATGCCACCTCAGCAATCTTCAAACTCTAGGTATTCGAAAATGTGGAGGTCTTGTTTCCATCCCGAGACTGAGTGAGGGGAGAAGACCCTCCAACCTGAGAAATATCGAGATCACCGATTGCGACAAATTGGAGGCGTTGCCCGAAGACATGCTCAATCTCAACTCTCTTGAGTACTTGAGCATCGACTACCGTGAAGGTCAAGAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1123

Amino Acids

127.02

Weight (kDa)

6.11

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 14 - 101 3.2e-18 Rx N-terminal domain
NB-ARC PF00931 173 - 347 1.3e-36 NB-ARC domain
WHD_DRP PF23559 430 - 499 2.5e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 573 - 868 1.9e-25 Leucine-rich repeat region
LRR_8 PF13855 580 - 635 6.3e-06 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 691 - 815 2.4e-36 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 978 - 1094 6.2e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 69, 2987
AccB1I GGYRCC 1 cut(s) 985
AccB7I CCANNNNNTGG 1 cut(s) 40
AccII CGCG 4 cut(s) 58, 63, 161, 1121
AciI CCGC 9 cut(s) 5, 161, 179, 239, 476, 809, 1119, 1741, 2241
AclWI GGATC 1 cut(s) 1321
AcoI YGGCCR 3 cut(s) 1116, 1341, 3001
AcsI RAATTY 3 cut(s) 729, 1307, 2312
AcuI CTGAAG 3 cut(s) 218, 1656, 3064
AcvI CACGTG 1 cut(s) 932
AcyI GRCGYC 3 cut(s) 66, 96, 2984
AfaI GTAC 9 cut(s) 77, 408, 639, 1557, 1736, 1864, 2789, 2954, 3332
AfiI CCNNNNNNNGG 4 cut(s) 40, 509, 2003, 2402
AflII CTTAAG 1 cut(s) 1076
AflIII ACRYGT 4 cut(s) 246, 851, 929, 2253
AhlI ACTAGT 1 cut(s) 1841
AjiI CACGTC 1 cut(s) 247
AjnI CCWGG 1 cut(s) 3011
AleI CACNNNNGTG 1 cut(s) 2574
Alw21I GWGCWC 4 cut(s) 904, 1749, 2286, 2763
Alw26I GTCTC 8 cut(s) 1202, 1225, 1795, 2214, 2504, 2835, 2900, 3217
Alw44I GTGCAC 1 cut(s) 2759
AlwI GGATC 1 cut(s) 1321
Ama87I CYCGRG 1 cut(s) 3220
AoxI GGCC 4 cut(s) 1116, 1341, 2084, 3001
ApaI GGGCCC 1 cut(s) 2088
ApaLI GTGCAC 1 cut(s) 2759
ApeKI GCWGC 8 cut(s) 544, 877, 962, 1024, 1505, 1777, 2533, 2768
ApoI RAATTY 3 cut(s) 729, 1307, 2312
ArsI GACNNNNNNTTYG 6 cut(s) 215, 247, 1292, 1324, 2813, 2845
Asp700I GAANNNNTTC 3 cut(s) 1675, 2550, 3074
AspLEI GCGC 4 cut(s) 58, 308, 1953, 2364
AspS9I GGNCC 4 cut(s) 500, 1125, 2084, 2085
AsuHPI GGTGA 6 cut(s) 875, 1499, 1805, 2278, 2836, 3262
AsuII TTCGAA 2 cut(s) 2819, 3193
AvaI CYCGRG 1 cut(s) 3220
AvaII GGWCC 2 cut(s) 500, 1125
BaeGI GKGCMC 3 cut(s) 463, 2088, 2763
BaeI ACNNNNGTAYC 4 cut(s) 927, 960, 2944, 2977
BalI TGGCCA 1 cut(s) 3003
BanI GGYRCC 1 cut(s) 985
BanII GRGCYC 2 cut(s) 2088, 2286
BbrPI CACGTG 1 cut(s) 932
BbsI GAAGAC 4 cut(s) 249, 2922, 3247, 3309
Bbv12I GWGCWC 4 cut(s) 904, 1749, 2286, 2763
BbvCI CCTCAGC 1 cut(s) 3167
BbvI GCAGC 8 cut(s) 531, 864, 949, 1036, 1517, 1764, 2545, 2755
BceAI ACGGC 1 cut(s) 662
BcgI CGANNNNNNTGC 6 cut(s) 569, 603, 1563, 1597, 2561, 2595
BciT130I CCWGG 1 cut(s) 3013
BcoDI GTCTC 8 cut(s) 1202, 1225, 1795, 2214, 2504, 2835, 2900, 3217
BcuI ACTAGT 1 cut(s) 1841
BfaI CTAG 8 cut(s) 480, 486, 1425, 1842, 1940, 2003, 2201, 3185
BfmI CTRYAG 2 cut(s) 878, 1923
BfrI CTTAAG 1 cut(s) 1076
BlpI GCTNAGC 1 cut(s) 2664
BmcAI AGTACT 7 cut(s) 77, 639, 1736, 1864, 2789, 2954, 3332
Bme1390I CCNGG 1 cut(s) 3013
Bme18I GGWCC 2 cut(s) 500, 1125
BmeT110I CYCGRG 1 cut(s) 3220
BmgBI CACGTC 1 cut(s) 247
BmgT120I GGNCC 4 cut(s) 500, 1125, 2084, 2085
BmiI GGNNCC 4 cut(s) 987, 1127, 2086, 2087
BmrFI CCNGG 1 cut(s) 3013
BmrI ACTGGG 2 cut(s) 450, 3062
BmsI GCATC 9 cut(s) 226, 571, 1259, 1308, 1461, 2104, 2124, 2140, 3348
BmuI ACTGGG 2 cut(s) 450, 3062
BpiI GAAGAC 4 cut(s) 249, 2922, 3247, 3309
BpmI CTGGAG 3 cut(s) 627, 1530, 2648
Bpu10I CCTNAGC 2 cut(s) 2772, 3167
Bpu1102I GCTNAGC 1 cut(s) 2664
Bpu14I TTCGAA 2 cut(s) 2819, 3193
BpuEI CTTGAG 7 cut(s) 2104, 2537, 2969, 2977, 3050, 3347, 3355
Bsa29I ATCGAT 1 cut(s) 1856
BsaAI YACGTR 1 cut(s) 932
BsaBI GATNNNNATC 2 cut(s) 258, 2974
BsaHI GRCGYC 3 cut(s) 66, 96, 2984
BsaI GGTCTC 1 cut(s) 2214
BsaJI CCNNGG 4 cut(s) 1129, 1344, 2101, 3011
BsaWI WCCGGW 1 cut(s) 3148
BsaXI ACNNNNNCTCC 2 cut(s) 3195, 3225
Bsc4I CCNNNNNNNGG 4 cut(s) 40, 509, 2003, 2402
Bse1I ACTGG 4 cut(s) 180, 445, 2631, 3057
Bse3DI GCAATG 1 cut(s) 1916
Bse8I GATNNNNATC 2 cut(s) 258, 2974
BseBI CCWGG 1 cut(s) 3013
BseCI ATCGAT 1 cut(s) 1856
BseDI CCNNGG 4 cut(s) 1129, 1344, 2101, 3011
BseGI GGATG 9 cut(s) 187, 876, 1344, 1357, 2155, 2900, 2975, 3157, 3216
BseJI GATNNNNATC 2 cut(s) 258, 2974
BseLI CCNNNNNNNGG 4 cut(s) 40, 509, 2003, 2402
BseMI GCAATG 1 cut(s) 1916
BseNI ACTGG 4 cut(s) 180, 445, 2631, 3057
BseRI GAGGAG 4 cut(s) 533, 2388, 2915, 2918
BseSI GKGCMC 3 cut(s) 463, 2088, 2763
BseXI GCAGC 8 cut(s) 531, 864, 949, 1036, 1517, 1764, 2545, 2755
Bsh1236I CGCG 4 cut(s) 58, 63, 161, 1121
BshFI GGCC 4 cut(s) 1118, 1343, 2086, 3003
BshNI GGYRCC 1 cut(s) 985
BshVI ATCGAT 1 cut(s) 1856
BsiHKAI GWGCWC 4 cut(s) 904, 1749, 2286, 2763
BsiHKCI CYCGRG 1 cut(s) 3220
BsiSI CCGG 2 cut(s) 2304, 3149
BslFI GGGAC 4 cut(s) 236, 457, 868, 2119
BslI CCNNNNNNNGG 4 cut(s) 40, 509, 2003, 2402
BsmAI GTCTC 8 cut(s) 1202, 1225, 1795, 2214, 2504, 2835, 2900, 3217
BsmFI GGGAC 4 cut(s) 236, 457, 868, 2119
BsmI GAATGC 3 cut(s) 1045, 1995, 2452
BsnI GGCC 4 cut(s) 1118, 1343, 2086, 3003
Bso31I GGTCTC 1 cut(s) 2214
BsoBI CYCGRG 1 cut(s) 3220
Bsp119I TTCGAA 2 cut(s) 2819, 3193
Bsp120I GGGCCC 1 cut(s) 2084
Bsp1286I GDGCHC 6 cut(s) 463, 904, 1749, 2088, 2286, 2763
Bsp1407I TGTACA 1 cut(s) 1555
Bsp143I GATC 7 cut(s) 214, 790, 1326, 2274, 2566, 2886, 3267
Bsp1720I GCTNAGC 1 cut(s) 2664
Bsp68I TCGCGA 1 cut(s) 63
BspACI CCGC 9 cut(s) 5, 161, 179, 239, 476, 809, 1119, 1741, 2241
BspANI GGCC 4 cut(s) 1118, 1343, 2086, 3003
BspDI ATCGAT 1 cut(s) 1856
BspFNI CGCG 4 cut(s) 58, 63, 161, 1121
BspLI GGNNCC 4 cut(s) 987, 1127, 2086, 2087
BspMAI CTGCAG 2 cut(s) 882, 1927
BspPI GGATC 1 cut(s) 1321
BspT104I TTCGAA 2 cut(s) 2819, 3193
BspT107I GGYRCC 1 cut(s) 985
BspTI CTTAAG 1 cut(s) 1076
BspTNI GGTCTC 1 cut(s) 2214
BsrDI GCAATG 1 cut(s) 1916
BsrGI TGTACA 1 cut(s) 1555
BsrI ACTGG 4 cut(s) 180, 445, 2631, 3057
BssECI CCNNGG 4 cut(s) 1129, 1344, 2101, 3011
BssMI GATC 7 cut(s) 214, 790, 1326, 2274, 2566, 2886, 3267
BssNI GRCGYC 3 cut(s) 66, 96, 2984
Bst2UI CCWGG 1 cut(s) 3013
Bst4CI ACNGT 5 cut(s) 1812, 2714, 2744, 2911, 3350
BstACI GRCGYC 3 cut(s) 66, 96, 2984
BstAFI CTTAAG 1 cut(s) 1076
BstAPI GCANNNNNTGC 1 cut(s) 1738
BstAUI TGTACA 1 cut(s) 1555
BstBAI YACGTR 1 cut(s) 932
BstBI TTCGAA 2 cut(s) 2819, 3193
BstC8I GCNNGC 5 cut(s) 463, 1029, 1576, 1923, 3005
BstEII GGTNACC 1 cut(s) 1705
BstF5I GGATG 9 cut(s) 187, 876, 1344, 1357, 2155, 2900, 2975, 3157, 3216
BstFNI CGCG 4 cut(s) 58, 63, 161, 1121
BstHHI GCGC 4 cut(s) 58, 308, 1953, 2364
BstKTI GATC 7 cut(s) 217, 793, 1329, 2277, 2569, 2889, 3270
BstMAI GTCTC 8 cut(s) 1202, 1225, 1795, 2214, 2504, 2835, 2900, 3217
BstMBI GATC 7 cut(s) 214, 790, 1326, 2274, 2566, 2886, 3267
BstNI CCWGG 1 cut(s) 3013
BstNSI RCATGY 3 cut(s) 1031, 2257, 3310
BstPI GGTNACC 1 cut(s) 1705
BstSCI CCNGG 1 cut(s) 3011
BstSFI CTRYAG 2 cut(s) 878, 1923
BstSLI GKGCMC 3 cut(s) 463, 2088, 2763
BstUI CGCG 4 cut(s) 58, 63, 161, 1121
BstV1I GCAGC 8 cut(s) 531, 864, 949, 1036, 1517, 1764, 2545, 2755
BstV2I GAAGAC 4 cut(s) 249, 2922, 3247, 3309
BstXI CCANNNNNNTGG 2 cut(s) 681, 2041
Bsu15I ATCGAT 1 cut(s) 1856
BsuRI GGCC 4 cut(s) 1118, 1343, 2086, 3003
BsuTUI ATCGAT 1 cut(s) 1856
BtrI CACGTC 1 cut(s) 247
BtsCI GGATG 9 cut(s) 187, 876, 1344, 1357, 2155, 2900, 2975, 3157, 3216
BtsI GCAGTG 2 cut(s) 2457, 3097
BtsIMutI CAGTG 3 cut(s) 2457, 2896, 3097
BtuMI TCGCGA 1 cut(s) 63
Cac8I GCNNGC 5 cut(s) 463, 1029, 1576, 1923, 3005
CfoI GCGC 4 cut(s) 58, 308, 1953, 2364
Cfr13I GGNCC 4 cut(s) 500, 1125, 2084, 2085
ClaI ATCGAT 1 cut(s) 1856
CseI GACGC 3 cut(s) 47, 85, 167
Csp6I GTAC 9 cut(s) 76, 407, 638, 1556, 1735, 1863, 2788, 2953, 3331
CviAII CATG 9 cut(s) 293, 321, 1028, 1211, 1475, 1930, 2254, 2322, 3307
CviQI GTAC 9 cut(s) 76, 407, 638, 1556, 1735, 1863, 2788, 2953, 3331
DpnI GATC 7 cut(s) 216, 792, 1328, 2276, 2568, 2888, 3269
DpnII GATC 7 cut(s) 214, 790, 1326, 2274, 2566, 2886, 3267
EaeI YGGCCR 3 cut(s) 1116, 1341, 3001
EciI GGCGGA 1 cut(s) 194
Ecl136II GAGCTC 1 cut(s) 2284
Eco24I GRGCYC 2 cut(s) 2088, 2286
Eco31I GGTCTC 1 cut(s) 2214
Eco32I GATATC 1 cut(s) 402
Eco47I GGWCC 2 cut(s) 500, 1125
Eco53kI GAGCTC 1 cut(s) 2284
Eco57I CTGAAG 3 cut(s) 218, 1656, 3064
Eco72I CACGTG 1 cut(s) 932
Eco88I CYCGRG 1 cut(s) 3220
Eco91I GGTNACC 1 cut(s) 1705
EcoICRI GAGCTC 1 cut(s) 2284
EcoO109I RGGNCCY 3 cut(s) 1125, 2084, 2085
EcoO65I GGTNACC 1 cut(s) 1705
EcoRI GAATTC 1 cut(s) 1307
EcoRII CCWGG 1 cut(s) 3011
EcoRV GATATC 1 cut(s) 402
EcoT22I ATGCAT 2 cut(s) 1033, 1476
EcoT38I GRGCYC 2 cut(s) 2088, 2286
FaeI CATG 9 cut(s) 296, 324, 1031, 1214, 1478, 1933, 2257, 2325, 3310
FalI AAGNNNNNCTT 2 cut(s) 2150, 2182
FaqI GGGAC 4 cut(s) 236, 457, 868, 2119
FatI CATG 9 cut(s) 292, 320, 1027, 1210, 1474, 1929, 2253, 2321, 3306
FauI CCCGC 2 cut(s) 802, 1734
FokI GGATG 9 cut(s) 194, 883, 1344, 1351, 2162, 2907, 2982, 3164, 3203
FriOI GRGCYC 2 cut(s) 2088, 2286
FspBI CTAG 8 cut(s) 480, 486, 1425, 1842, 1940, 2003, 2201, 3185
GlaI GCGC 4 cut(s) 57, 307, 1952, 2363
GsuI CTGGAG 3 cut(s) 627, 1530, 2648
HaeIII GGCC 4 cut(s) 1118, 1343, 2086, 3003
HapII CCGG 2 cut(s) 2304, 3149
HgaI GACGC 3 cut(s) 47, 85, 167
HhaI GCGC 4 cut(s) 58, 308, 1953, 2364
Hin1I GRCGYC 3 cut(s) 66, 96, 2984
Hin1II CATG 9 cut(s) 296, 324, 1031, 1214, 1478, 1933, 2257, 2325, 3310
Hin6I GCGC 4 cut(s) 56, 306, 1951, 2362
HinP1I GCGC 4 cut(s) 56, 306, 1951, 2362
HincII GTYRAC 2 cut(s) 46, 450
HindII GTYRAC 2 cut(s) 46, 450
HindIII AAGCTT 1 cut(s) 2666
HpaII CCGG 2 cut(s) 2304, 3149
HphI GGTGA 6 cut(s) 875, 1499, 1805, 2278, 2836, 3262
Hpy166II GTNNAC 9 cut(s) 46, 392, 450, 976, 1556, 2636, 2717, 2761, 3047
Hpy8I GTNNAC 9 cut(s) 46, 392, 450, 976, 1556, 2636, 2717, 2761, 3047
Hpy99I CGWCG 4 cut(s) 63, 68, 101, 2989
HpyCH4III ACNGT 5 cut(s) 1812, 2714, 2744, 2911, 3350
HpyCH4IV ACGT 7 cut(s) 66, 246, 280, 446, 851, 931, 2984
HpySE526I ACGT 7 cut(s) 66, 246, 280, 446, 851, 931, 2984
Hsp92I GRCGYC 3 cut(s) 66, 96, 2984
Hsp92II CATG 9 cut(s) 296, 324, 1031, 1214, 1478, 1933, 2257, 2325, 3310
HspAI GCGC 4 cut(s) 56, 306, 1951, 2362
Kzo9I GATC 7 cut(s) 214, 790, 1326, 2274, 2566, 2886, 3267
LmnI GCTCC 5 cut(s) 143, 899, 959, 1414, 2281
Lsp1109I GCAGC 8 cut(s) 531, 864, 949, 1036, 1517, 1764, 2545, 2755
LweI GCATC 9 cut(s) 226, 571, 1259, 1308, 1461, 2104, 2124, 2140, 3348
MaeI CTAG 8 cut(s) 480, 486, 1425, 1842, 1940, 2003, 2201, 3185
MaeII ACGT 7 cut(s) 66, 246, 280, 446, 851, 931, 2984
MaeIII GTNAC 8 cut(s) 718, 922, 1071, 1238, 1705, 2134, 2256, 2375
MalI GATC 7 cut(s) 216, 792, 1328, 2276, 2568, 2888, 3269
MboI GATC 7 cut(s) 214, 790, 1326, 2274, 2566, 2886, 3267
MfeI CAATTG 5 cut(s) 138, 591, 2380, 2408, 2703
MhlI GDGCHC 6 cut(s) 463, 904, 1749, 2088, 2286, 2763
MlsI TGGCCA 1 cut(s) 3003
MluNI TGGCCA 1 cut(s) 3003
MlyI GAGTC 5 cut(s) 78, 618, 1204, 2506, 2804
MmeI TCCRAC 5 cut(s) 231, 592, 1962, 2308, 3273
Mox20I TGGCCA 1 cut(s) 3003
Mph1103I ATGCAT 2 cut(s) 1033, 1476
MroXI GAANNNNTTC 3 cut(s) 1675, 2550, 3074
MscI TGGCCA 1 cut(s) 3003
MslI CAYNNNNRTG 2 cut(s) 1605, 2574
Msp20I TGGCCA 1 cut(s) 3003
MspA1I CMGCKG 2 cut(s) 1508, 2768
MspCI CTTAAG 1 cut(s) 1076
MspI CCGG 2 cut(s) 2304, 3149
MspR9I CCNGG 1 cut(s) 3013
MunI CAATTG 5 cut(s) 138, 591, 2380, 2408, 2703
Mva1269I GAATGC 3 cut(s) 1045, 1995, 2452
MvaI CCWGG 1 cut(s) 3013
MvnI CGCG 4 cut(s) 58, 63, 161, 1121
NdeII GATC 7 cut(s) 214, 790, 1326, 2274, 2566, 2886, 3267
NlaIII CATG 9 cut(s) 296, 324, 1031, 1214, 1478, 1933, 2257, 2325, 3310
NlaIV GGNNCC 4 cut(s) 987, 1127, 2086, 2087
NmeAIII GCCGAG 2 cut(s) 730, 1369
NmuCI GTSAC 3 cut(s) 718, 922, 2134
NruI TCGCGA 1 cut(s) 63
NsiI ATGCAT 2 cut(s) 1033, 1476
NspI RCATGY 3 cut(s) 1031, 2257, 3310
NspV TTCGAA 2 cut(s) 2819, 3193
OliI CACNNNNGTG 1 cut(s) 2574
PaeI GCATGC 1 cut(s) 1031
PciI ACATGT 1 cut(s) 2253
PcsI WCGNNNNNNNCGW 2 cut(s) 93, 3270
PctI GAATGC 3 cut(s) 1045, 1995, 2452
PdmI GAANNNNTTC 3 cut(s) 1675, 2550, 3074
PfeI GAWTC 6 cut(s) 1354, 2017, 2063, 2189, 2237, 2975
PflMI CCANNNNNTGG 1 cut(s) 40
PleI GAGTC 5 cut(s) 78, 617, 1203, 2506, 2803
PmaCI CACGTG 1 cut(s) 932
PmlI CACGTG 1 cut(s) 932
PpsI GAGTC 5 cut(s) 78, 617, 1203, 2506, 2803
Ppu21I YACGTR 1 cut(s) 932
PpuMI RGGWCCY 1 cut(s) 1125
PscI ACATGT 1 cut(s) 2253
Psp124BI GAGCTC 1 cut(s) 2286
Psp5II RGGWCCY 1 cut(s) 1125
Psp6I CCWGG 1 cut(s) 3011
PspCI CACGTG 1 cut(s) 932
PspEI GGTNACC 1 cut(s) 1705
PspGI CCWGG 1 cut(s) 3011
PspN4I GGNNCC 4 cut(s) 987, 1127, 2086, 2087
PspOMI GGGCCC 1 cut(s) 2084
PspPI GGNCC 4 cut(s) 500, 1125, 2084, 2085
PspPPI RGGWCCY 1 cut(s) 1125
PstI CTGCAG 2 cut(s) 882, 1927
PvuII CAGCTG 2 cut(s) 1508, 2768
RruI TCGCGA 1 cut(s) 63
RsaI GTAC 9 cut(s) 77, 408, 639, 1557, 1736, 1864, 2789, 2954, 3332
RsaNI GTAC 9 cut(s) 76, 407, 638, 1556, 1735, 1863, 2788, 2953, 3331
RseI CAYNNNNRTG 2 cut(s) 1605, 2574
SacI GAGCTC 1 cut(s) 2286
Sau3AI GATC 7 cut(s) 214, 790, 1326, 2274, 2566, 2886, 3267
Sau96I GGNCC 4 cut(s) 500, 1125, 2084, 2085
ScaI AGTACT 7 cut(s) 77, 639, 1736, 1864, 2789, 2954, 3332
SchI GAGTC 5 cut(s) 78, 618, 1204, 2506, 2804
ScrFI CCNGG 1 cut(s) 3013
SduI GDGCHC 6 cut(s) 463, 904, 1749, 2088, 2286, 2763
SfaNI GCATC 9 cut(s) 226, 571, 1259, 1308, 1461, 2104, 2124, 2140, 3348
SfcI CTRYAG 2 cut(s) 878, 1923
SfuI TTCGAA 2 cut(s) 2819, 3193
SinI GGWCC 2 cut(s) 500, 1125
SmiMI CAYNNNNRTG 2 cut(s) 1605, 2574
SmlI CTYRAG 8 cut(s) 1076, 2119, 2516, 2948, 2956, 3029, 3326, 3334
SmoI CTYRAG 8 cut(s) 1076, 2119, 2516, 2948, 2956, 3029, 3326, 3334
SpeI ACTAGT 1 cut(s) 1841
SphI GCATGC 1 cut(s) 1031
SsiI CCGC 9 cut(s) 5, 161, 179, 239, 476, 809, 1119, 1741, 2241
SspI AATATT 2 cut(s) 1176, 1456
SspMI CTAG 8 cut(s) 480, 486, 1425, 1842, 1940, 2003, 2201, 3185
SstI GAGCTC 1 cut(s) 2286
StyD4I CCNGG 1 cut(s) 3011
TaaI ACNGT 5 cut(s) 1812, 2714, 2744, 2911, 3350
TaiI ACGT 7 cut(s) 69, 249, 283, 449, 854, 934, 2987
TatI WGTACW 8 cut(s) 75, 637, 1555, 1734, 1862, 2787, 2952, 3330
TauI GCSGC 2 cut(s) 8, 1121
TfiI GAWTC 6 cut(s) 1354, 2017, 2063, 2189, 2237, 2975
TscAI CASTG 3 cut(s) 2457, 2896, 3104
TseFI GTSAC 3 cut(s) 718, 922, 2134
TseI GCWGC 8 cut(s) 544, 877, 962, 1024, 1505, 1777, 2533, 2768
Tsp45I GTSAC 3 cut(s) 718, 922, 2134
TspDTI ATGAA 7 cut(s) 374, 887, 1320, 1346, 2076, 2307, 2459
TspGWI ACGGA 1 cut(s) 128
TspRI CASTG 3 cut(s) 2457, 2896, 3104
Van91I CCANNNNNTGG 1 cut(s) 40
Vha464I CTTAAG 1 cut(s) 1076
VneI GTGCAC 1 cut(s) 2759
VpaK11BI GGWCC 2 cut(s) 500, 1125
XapI RAATTY 3 cut(s) 729, 1307, 2312
XbaI TCTAGA 1 cut(s) 2200
XceI RCATGY 3 cut(s) 1031, 2257, 3310
XmnI GAANNNNTTC 3 cut(s) 1675, 2550, 3074
XspI CTAG 8 cut(s) 480, 486, 1425, 1842, 1940, 2003, 2201, 3185
ZraI GACGTC 2 cut(s) 67, 2985
ZrmI AGTACT 7 cut(s) 77, 639, 1736, 1864, 2789, 2954, 3332
Zsp2I ATGCAT 2 cut(s) 1033, 1476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.