RchiOBHm_Chr1g0320661

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000070
Physical Location & Seq
Reverse (-)
8272513 .. 8275495
2983 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55079

Sequence Viewer

Length: 2880 bp
ATGGCATTGGGAGAGGTCTTTCTTGCGGCATTCCTTCAGTTGCTGCTGGACAGGTTAACGCCTCGAGAGATCCTGGGCTACTTTGGAAGCTTGGGAGGAGCTGGCAAAAAGCTGCAAAAATGGAGAGAGACACTTTCCGCAGTTGCAGCGGTGCTTTCCGATGCAGAGGAGAAGCAGCTGACGAACAAGGCAGTGGATCTGTGGCTGAATGACCTCAAACACTTGGCTTATGATATCGACGATCTACTCAACACCTTTTCTAATGAGATGTTAGCACGCAAGCAGCACAAGCTTCACCAAACTTGCACAAGCAAGGTACGCCGCTTCTTTACCAAAGTTCCTCAAAAAGTCAAGTTCAATTTCAATATGAGCTCCGAAATAGACGACATTGCTAACCGCTTGCAAGACATATTTGACCGGAAAGTGAAACTCAGTTTGAATACCTCTACTTCAACATCCTCATCGCAAAGGACACCAAGTTCGTATGTGTTGGATGGACCTGTGGTTGGGAGACATGAAGACGCAAGAAAGATTGTTGACCTGTTGTCCAGAGATGTTGATCCTTCTTCTACCACCAATTATCAAGTTGTTGCCATTGTTGGTATGGGAGGACTCGGCAAGACAACGCTTGCGGGACAAGTCTTCAATGATGTAGCAGCAATGGAACAGTTTGATCTCAAGATCTGGGTTTCAGTGTCCGATGACTTCAATCTTCAAACGGTGACGAGAACTATTACTAAGGAAGTCACATCTCGGCCATGTGATATGGATGAGTTCAGTCGACTTCAAGATAATCTGAGCAAGGCGATAGATGGTAAAAGGTTTCTGATTGTTTTAGATGATGTCTGGAGCACATGTGATTATGATTCATGGACAAAACTGCAAGCTCCCTTTCGTAGGGGAGCTAAGGGAAGCAAGGTAATGGTGACAACACGTGAGGAAAAAGTTGCTGCATTGATGGGAGCCCCAGCCCAAGCCCCAGCCACTGAAGTATATCATTTAGAGACTTTATCAGAAGAAGATTGTTTGCAAGTATTTGAGCAGCATGTTAGCAATGACAGGCCACCAAATTTTGACTTGCTTAAGAAGAAAATTGTAACGAACTGCAATGGATTGCCATTGGCTGCAAAAACTCTTGGTGGTGTTTTACGTTGTAACAAAACTGACAAGTGGGAGGAAATACTGAATGATAAATTATGGAGCATATCAGATGGGAGTAATATACTCCCAGTACTCAGATTGAGTTATCATTATCTCCCTTCAACTTTGAAGAGGTGTTTTGCCTATTGCTCAATACTTCCCAATGACTATAAATTTGGGAAAACACAATTAATCCTGCTGTGGATGGCAGAGGGTTTCCTTGCTTTACCAGAAGGGACTAAAGCAATGGAAGATATCGGTGATGAATATTTTGGGGAGCTACTATCTCGGTCATTATTTCAAAACTCGGGCAAAAACAGTTCATGCTATGTAATGCATGACCTTGTTGGTGAGTTGGCACGATGGGCTGCTGGAGACACATTTTGCAGATTGGAGGATAAACTGCATGGTACATGTTCTGCAAAGACTCGTCATCTGGCTTACATTTCTGGTAAGTTCGATGGGGTAAAAAGATTTGAGACAATTTCTGAAGCAAAACGCTTGCGGACTTTCCTACCACTTCCTGTTTCTGGTGGTTATGAGAATTATCTGACTCGTTATGCAACTTCTAATTTATTGCCACAATTGAAATACTTGCGGGTACTCTCCTTCAATGGCTATAAACTGACCGAGCTGCCAGATTCCGTAGGTAAGTTGAGACATCTGCGGTACCTTGATCTTTCTGACACATTAATAATGAGTTTGCCTGAGTCAACATGCATGCTTTACAACTTACAGACGTTAATATTAGAGAATTGTTCAAAATTGAAGACACTACCTTCAAACATGAGCAATTTATCTAATTTACGTCATCTCAACAATTCAAACACGCCTTCATTGGAAGAAATGCCTCCCCAAGTAAGTCGGTTGACTCATCTGCAAACTGTGCCTAATTTTGTGGTGGGGAAATGTAGTGCATCAGGGCTTGGAGAGATAGGGTCATTGCATCTTCGTGGGACCATAAGCCTTGGAAGATTGGAAAATGTGATCGATGTTGGGGATGTGAAGAGGGCCAAAATAATGAATAAGGAAGGGCTGGATACATTGCAATTGGAATGGAGTGGCACAAGTGAGAAGGAGTCGGAGGTGCTCTGCAGTTTAGAACCTCATAAAAAGCTTCTAGACCTCACCATCAAGGGCTACAATGGTTTCGAATTTTCAAAATGGATTGGACATCCTTCATTCTCTGATATGACGCATGTGAAGTTAGAGAATTGTAAAAATTGTCGATTCTTACCTCCTCTTGGCCAATTACCTTTTCTGAAAAAACTTCAGATACAAGGACTGGCCAATGTTAAAAGTGTGGGTGCTGAGTTTTATGGAGAGTGCAGCTTGCCTTTTCCAGTGTTGGAAGACCTGAGGTTCGAGGATATGCAAAATTGGAAGGAGTGGCTTCCTTGCAAAACAAATGAAGAAATTCGAGTTTTCCCTTGCCTGCGACAGGTTTCCATCTCTAGATGCCCGAAATTGAAGTGTTTGCTATCCAAGAGCATGGATTCATTATCAGAGCTGTATATTGCTTTATGTGAAGAGTTAGTGGTTTCAATTGCCAATTATAAACATCTTCGTGGGCTGTTCATCTGCTGTTGTAAAAGGGTGGTGCACAGAAGTGGAGTTAAGTTTGAGCTACTGGAGGATATGGAGCTTCATGGTATTTCAGAGTTCAGACTCGAAATAGACGGGTTCATCAGAGGATTGCCAAAGCTTCAAGGATTGTGGATTACTGGTTGTGAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

959

Amino Acids

108.14

Weight (kDa)

7.67

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 98 1e-18 Rx N-terminal domain
NB-ARC PF00931 192 - 352 1.4e-38 NB-ARC domain
WHD_DRP PF23559 432 - 500 4.9e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 573 - 841 1.3e-21 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 690 - 808 7.5e-32 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2697
Acc65I GGTACC 1 cut(s) 1809
AccB1I GGYRCC 1 cut(s) 1809
AccI GTMKAC 1 cut(s) 781
AciI CCGC 9 cut(s) 26, 138, 149, 322, 397, 632, 1645, 1738, 1807
AclWI GGATC 3 cut(s) 64, 204, 554
AcoI YGGCCR 3 cut(s) 755, 2386, 2427
AcsI RAATTY 4 cut(s) 1069, 1313, 2294, 2556
AcuI CTGAAG 4 cut(s) 20, 1008, 1650, 2396
AcvI CACGTG 1 cut(s) 935
AfaI GTAC 5 cut(s) 318, 1233, 1552, 1743, 1811
AfiI CCNNNNNNNGG 5 cut(s) 506, 897, 1670, 2384, 2581
AflII CTTAAG 1 cut(s) 1082
AflIII ACRYGT 3 cut(s) 854, 932, 1553
AhdI GACNNNNNGTC 1 cut(s) 544
AjnI CCWGG 1 cut(s) 72
AjuI GAANNNNNNNTTGG 2 cut(s) 2382, 2414
AleI CACNNNNGTG 1 cut(s) 2748
AloI GAACNNNNNNTCC 2 cut(s) 463, 495
Alw21I GWGCWC 4 cut(s) 374, 854, 2232, 2745
Alw26I GTCTC 6 cut(s) 122, 505, 998, 1509, 1613, 1792
Alw44I GTGCAC 1 cut(s) 2741
AlwI GGATC 3 cut(s) 64, 204, 554
AlwNI CAGNNNCTG 2 cut(s) 43, 986
Ama87I CYCGRG 2 cut(s) 63, 1447
AoxI GGCC 5 cut(s) 755, 1061, 2151, 2386, 2427
ApaLI GTGCAC 1 cut(s) 2741
ApoI RAATTY 4 cut(s) 1069, 1313, 2294, 2556
ArsI GACNNNNNNTTYG 6 cut(s) 463, 495, 1298, 1330, 2486, 2518
AseI ATTAAT 2 cut(s) 1331, 1832
Asp700I GAANNNNTTC 1 cut(s) 2556
Asp718I GGTACC 1 cut(s) 1809
AspS9I GGNCC 3 cut(s) 497, 2097, 2151
AsuHPI GGTGA 6 cut(s) 287, 733, 937, 1412, 1502, 2260
AsuII TTCGAA 1 cut(s) 2292
AvaI CYCGRG 2 cut(s) 63, 1447
AvaII GGWCC 2 cut(s) 497, 2097
AxyI CCTNAGG 1 cut(s) 2498
BaeGI GKGCMC 1 cut(s) 2745
BalI TGGCCA 2 cut(s) 2388, 2429
BanI GGYRCC 1 cut(s) 1809
BanII GRGCYC 2 cut(s) 374, 967
BbrPI CACGTG 1 cut(s) 935
BbsI GAAGAC 4 cut(s) 525, 634, 1916, 2499
Bbv12I GWGCWC 4 cut(s) 374, 854, 2232, 2745
BccI CCATC 9 cut(s) 488, 806, 952, 1205, 1339, 1497, 1595, 2279, 2597
BciT130I CCWGG 1 cut(s) 74
BciVI GTATCC 1 cut(s) 2173
BcoDI GTCTC 6 cut(s) 122, 505, 998, 1509, 1613, 1792
BfaI CTAG 2 cut(s) 2261, 2595
BfmI CTRYAG 1 cut(s) 2233
BfrI CTTAAG 1 cut(s) 1082
BfuI GTATCC 1 cut(s) 2173
BglII AGATCT 1 cut(s) 681
BmcAI AGTACT 1 cut(s) 1233
Bme1390I CCNGG 1 cut(s) 74
Bme18I GGWCC 2 cut(s) 497, 2097
BmeRI GACNNNNNGTC 1 cut(s) 544
BmeT110I CYCGRG 2 cut(s) 63, 1447
BmgT120I GGNCC 3 cut(s) 497, 2097, 2151
BmiI GGNNCC 3 cut(s) 964, 1811, 2098
BmrFI CCNGG 1 cut(s) 74
BmrI ACTGGG 1 cut(s) 1223
BmsI GCATC 4 cut(s) 151, 2066, 2095, 2588
BmuI ACTGGG 1 cut(s) 1223
BpiI GAAGAC 4 cut(s) 525, 634, 1916, 2499
BpmI CTGGAG 3 cut(s) 868, 1533, 2792
Bpu10I CCTNAGC 1 cut(s) 906
Bpu14I TTCGAA 1 cut(s) 2292
BpuEI CTTGAG 1 cut(s) 662
Bsa29I ATCGAT 1 cut(s) 2130
BsaAI YACGTR 1 cut(s) 935
BsaBI GATNNNNATC 1 cut(s) 558
BsaJI CCNNGG 2 cut(s) 73, 2107
BsaWI WCCGGW 1 cut(s) 417
BsaXI ACNNNNNCTCC 2 cut(s) 841, 871
Bsc4I CCNNNNNNNGG 5 cut(s) 506, 897, 1670, 2384, 2581
Bse1I ACTGG 5 cut(s) 1229, 2430, 2483, 2775, 2869
Bse21I CCTNAGG 1 cut(s) 2498
Bse3DI GCAATG 7 cut(s) 387, 666, 1060, 1114, 1392, 2081, 2183
Bse8I GATNNNNATC 1 cut(s) 558
BseBI CCWGG 1 cut(s) 74
BseCI ATCGAT 1 cut(s) 2130
BseDI CCNNGG 2 cut(s) 73, 2107
BseGI GGATG 6 cut(s) 455, 499, 775, 1350, 2146, 2314
BseJI GATNNNNATC 1 cut(s) 558
BseLI CCNNNNNNNGG 5 cut(s) 506, 897, 1670, 2384, 2581
BseMI GCAATG 7 cut(s) 387, 666, 1060, 1114, 1392, 2081, 2183
BseMII CTCAG 6 cut(s) 445, 788, 1249, 1839, 2442, 2489
BseNI ACTGG 5 cut(s) 1229, 2430, 2483, 2775, 2869
BseRI GAGGAG 3 cut(s) 111, 182, 2370
BseSI GKGCMC 1 cut(s) 2745
BseYI CCCAGC 2 cut(s) 967, 979
BsgI GTGCAG 1 cut(s) 2488
BshFI GGCC 5 cut(s) 757, 1063, 2153, 2388, 2429
BshNI GGYRCC 1 cut(s) 1809
BshVI ATCGAT 1 cut(s) 2130
BsiHKAI GWGCWC 4 cut(s) 374, 854, 2232, 2745
BsiHKCI CYCGRG 2 cut(s) 63, 1447
BsiSI CCGG 1 cut(s) 418
BslFI GGGAC 3 cut(s) 648, 1390, 2110
BslI CCNNNNNNNGG 5 cut(s) 506, 897, 1670, 2384, 2581
BsmAI GTCTC 6 cut(s) 122, 505, 998, 1509, 1613, 1792
BsmFI GGGAC 3 cut(s) 648, 1390, 2110
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 5 cut(s) 757, 1063, 2153, 2388, 2429
BsoBI CYCGRG 2 cut(s) 63, 1447
Bsp119I TTCGAA 1 cut(s) 2292
Bsp1286I GDGCHC 5 cut(s) 374, 854, 967, 2232, 2745
Bsp143I GATC 8 cut(s) 69, 196, 241, 559, 673, 681, 1816, 2127
BspACI CCGC 9 cut(s) 26, 138, 149, 322, 397, 632, 1645, 1738, 1807
BspANI GGCC 5 cut(s) 757, 1063, 2153, 2388, 2429
BspCNI CTCAG 6 cut(s) 444, 789, 1248, 1840, 2443, 2490
BspDI ATCGAT 1 cut(s) 2130
BspLI GGNNCC 3 cut(s) 964, 1811, 2098
BspMAI CTGCAG 1 cut(s) 2237
BspPI GGATC 3 cut(s) 64, 204, 554
BspT104I TTCGAA 1 cut(s) 2292
BspT107I GGYRCC 1 cut(s) 1809
BspTI CTTAAG 1 cut(s) 1082
BsrDI GCAATG 7 cut(s) 387, 666, 1060, 1114, 1392, 2081, 2183
BsrI ACTGG 5 cut(s) 1229, 2430, 2483, 2775, 2869
BssECI CCNNGG 2 cut(s) 73, 2107
BssMI GATC 8 cut(s) 69, 196, 241, 559, 673, 681, 1816, 2127
BssT1I CCWWGG 1 cut(s) 2107
Bst2UI CCWGG 1 cut(s) 74
Bst4CI ACNGT 4 cut(s) 669, 721, 1460, 2026
Bst6I CTCTTC 3 cut(s) 1265, 2141, 2664
BstAFI CTTAAG 1 cut(s) 1082
BstAPI GCANNNNNTGC 1 cut(s) 2026
BstBAI YACGTR 1 cut(s) 935
BstBI TTCGAA 1 cut(s) 2292
BstDEI CTNAG 8 cut(s) 431, 738, 797, 906, 1235, 1848, 2451, 2498
BstENI CCTNNNNNAGG 2 cut(s) 895, 2579
BstF5I GGATG 6 cut(s) 455, 499, 775, 1350, 2146, 2314
BstKTI GATC 8 cut(s) 72, 199, 244, 562, 676, 684, 1819, 2130
BstMAI GTCTC 6 cut(s) 122, 505, 998, 1509, 1613, 1792
BstMBI GATC 8 cut(s) 69, 196, 241, 559, 673, 681, 1816, 2127
BstMWI GCNNNNNNNGC 5 cut(s) 146, 289, 318, 1505, 2026
BstNI CCWGG 1 cut(s) 74
BstNSI RCATGY 6 cut(s) 858, 1049, 1557, 1860, 1864, 2342
BstSCI CCNGG 1 cut(s) 72
BstSFI CTRYAG 1 cut(s) 2233
BstSLI GKGCMC 1 cut(s) 2745
BstV2I GAAGAC 4 cut(s) 525, 634, 1916, 2499
BstX2I RGATCY 3 cut(s) 69, 196, 681
BstXI CCANNNNNNTGG 1 cut(s) 2632
BstYI RGATCY 3 cut(s) 69, 196, 681
Bsu15I ATCGAT 1 cut(s) 2130
Bsu36I CCTNAGG 1 cut(s) 2498
BsuI GTATCC 1 cut(s) 2173
BsuRI GGCC 5 cut(s) 757, 1063, 2153, 2388, 2429
BsuTUI ATCGAT 1 cut(s) 2130
BtgZI GCGATG 1 cut(s) 447
BtsCI GGATG 6 cut(s) 455, 499, 775, 1350, 2146, 2314
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 4 cut(s) 198, 699, 984, 2490
CaiI CAGNNNCTG 2 cut(s) 43, 986
Cfr13I GGNCC 3 cut(s) 497, 2097, 2151
ClaI ATCGAT 1 cut(s) 2130
CseI GACGC 2 cut(s) 530, 2344
Csp6I GTAC 5 cut(s) 317, 1232, 1551, 1742, 1810
CspCI CAANNNNNGTGG 2 cut(s) 2837, 2872
CviQI GTAC 5 cut(s) 317, 1232, 1551, 1742, 1810
DdeI CTNAG 8 cut(s) 431, 738, 797, 906, 1235, 1848, 2451, 2498
DpnI GATC 8 cut(s) 71, 198, 243, 561, 675, 683, 1818, 2129
DpnII GATC 8 cut(s) 69, 196, 241, 559, 673, 681, 1816, 2127
DriI GACNNNNNGTC 1 cut(s) 544
EaeI YGGCCR 3 cut(s) 755, 2386, 2427
Eam1104I CTCTTC 3 cut(s) 1265, 2141, 2664
Eam1105I GACNNNNNGTC 1 cut(s) 544
EarI CTCTTC 3 cut(s) 1265, 2141, 2664
Ecl136II GAGCTC 1 cut(s) 372
Eco130I CCWWGG 1 cut(s) 2107
Eco24I GRGCYC 2 cut(s) 374, 967
Eco32I GATATC 2 cut(s) 235, 1396
Eco47I GGWCC 2 cut(s) 497, 2097
Eco53kI GAGCTC 1 cut(s) 372
Eco57I CTGAAG 4 cut(s) 20, 1008, 1650, 2396
Eco72I CACGTG 1 cut(s) 935
Eco81I CCTNAGG 1 cut(s) 2498
Eco88I CYCGRG 2 cut(s) 63, 1447
EcoICRI GAGCTC 1 cut(s) 372
EcoNI CCTNNNNNAGG 2 cut(s) 895, 2579
EcoRII CCWGG 1 cut(s) 72
EcoRV GATATC 2 cut(s) 235, 1396
EcoT14I CCWWGG 1 cut(s) 2107
EcoT22I ATGCAT 2 cut(s) 1479, 1862
EcoT38I GRGCYC 2 cut(s) 374, 967
ErhI CCWWGG 1 cut(s) 2107
FaqI GGGAC 3 cut(s) 648, 1390, 2110
FauI CCCGC 2 cut(s) 625, 1731
FblI GTMKAC 1 cut(s) 781
FokI GGATG 6 cut(s) 442, 506, 782, 1357, 2153, 2301
FriOI GRGCYC 2 cut(s) 374, 967
FspBI CTAG 2 cut(s) 2261, 2595
GsaI CCCAGC 2 cut(s) 971, 983
GsuI CTGGAG 3 cut(s) 868, 1533, 2792
HaeIII GGCC 5 cut(s) 757, 1063, 2153, 2388, 2429
HapII CCGG 1 cut(s) 418
HgaI GACGC 2 cut(s) 530, 2344
HincII GTYRAC 5 cut(s) 57, 538, 782, 1854, 2010
HindII GTYRAC 5 cut(s) 57, 538, 782, 1854, 2010
HindIII AAGCTT 4 cut(s) 88, 290, 2255, 2843
HpaI GTTAAC 1 cut(s) 57
HpaII CCGG 1 cut(s) 418
HphI GGTGA 6 cut(s) 287, 733, 937, 1412, 1502, 2260
Hpy166II GTNNAC 6 cut(s) 57, 538, 782, 1854, 2010, 2743
Hpy188III TCNNGA 7 cut(s) 65, 549, 679, 788, 847, 2261, 2595
Hpy8I GTNNAC 6 cut(s) 57, 538, 782, 1854, 2010, 2743
Hpy99I CGWCG 1 cut(s) 242
HpyCH4III ACNGT 4 cut(s) 669, 721, 1460, 2026
HpyCH4IV ACGT 4 cut(s) 934, 1150, 1880, 1948
HpyF10VI GCNNNNNNNGC 5 cut(s) 146, 289, 318, 1505, 2026
HpyF3I CTNAG 8 cut(s) 431, 738, 797, 906, 1235, 1848, 2451, 2498
HpySE526I ACGT 4 cut(s) 934, 1150, 1880, 1948
KpnI GGTACC 1 cut(s) 1813
KspAI GTTAAC 1 cut(s) 57
Kzo9I GATC 8 cut(s) 69, 196, 241, 559, 673, 681, 1816, 2127
LmnI GCTCC 9 cut(s) 98, 377, 849, 892, 902, 962, 1200, 1417, 2782
LweI GCATC 4 cut(s) 151, 2066, 2095, 2588
MaeI CTAG 2 cut(s) 2261, 2595
MaeII ACGT 4 cut(s) 934, 1150, 1880, 1948
MaeIII GTNAC 5 cut(s) 721, 745, 925, 1096, 1154
MalI GATC 8 cut(s) 71, 198, 243, 561, 675, 683, 1818, 2129
MboI GATC 8 cut(s) 69, 196, 241, 559, 673, 681, 1816, 2127
MfeI CAATTG 3 cut(s) 1724, 2189, 2685
MflI RGATCY 3 cut(s) 69, 196, 681
MhlI GDGCHC 5 cut(s) 374, 854, 967, 2232, 2745
MlsI TGGCCA 2 cut(s) 2388, 2429
MluNI TGGCCA 2 cut(s) 2388, 2429
MlyI GAGTC 7 cut(s) 606, 1561, 1687, 1859, 2005, 2228, 2802
MmeI TCCRAC 3 cut(s) 471, 2202, 2469
Mox20I TGGCCA 2 cut(s) 2388, 2429
Mph1103I ATGCAT 2 cut(s) 1479, 1862
MroXI GAANNNNTTC 1 cut(s) 2556
MscI TGGCCA 2 cut(s) 2388, 2429
MseI TTAA 7 cut(s) 56, 1083, 1331, 1832, 1883, 2436, 2757
MslI CAYNNNNRTG 3 cut(s) 2091, 2706, 2748
Msp20I TGGCCA 2 cut(s) 2388, 2429
MspA1I CMGCKG 2 cut(s) 149, 178
MspCI CTTAAG 1 cut(s) 1082
MspI CCGG 1 cut(s) 418
MspR9I CCNGG 1 cut(s) 74
MunI CAATTG 3 cut(s) 1724, 2189, 2685
Mva1269I GAATGC 1 cut(s) 29
MvaI CCWGG 1 cut(s) 74
MwoI GCNNNNNNNGC 5 cut(s) 146, 289, 318, 1505, 2026
NdeII GATC 8 cut(s) 69, 196, 241, 559, 673, 681, 1816, 2127
NlaIV GGNNCC 3 cut(s) 964, 1811, 2098
NmeAIII GCCGAG 2 cut(s) 594, 733
NmuCI GTSAC 3 cut(s) 721, 745, 925
NsiI ATGCAT 2 cut(s) 1479, 1862
NspI RCATGY 6 cut(s) 858, 1049, 1557, 1860, 1864, 2342
NspV TTCGAA 1 cut(s) 2292
OliI CACNNNNGTG 1 cut(s) 2748
PaeI GCATGC 1 cut(s) 1864
PaeR7I CTCGAG 1 cut(s) 63
PciI ACATGT 2 cut(s) 854, 1553
PctI GAATGC 1 cut(s) 29
PdmI GAANNNNTTC 1 cut(s) 2556
PfeI GAWTC 4 cut(s) 866, 1781, 2370, 2636
PleI GAGTC 7 cut(s) 606, 1561, 1687, 1858, 2005, 2227, 2802
PmaCI CACGTG 1 cut(s) 935
PmlI CACGTG 1 cut(s) 935
PpsI GAGTC 7 cut(s) 606, 1561, 1687, 1858, 2005, 2227, 2802
Ppu21I YACGTR 1 cut(s) 935
PscI ACATGT 2 cut(s) 854, 1553
PshBI ATTAAT 2 cut(s) 1331, 1832
PsiI TTATAA 1 cut(s) 2697
Psp124BI GAGCTC 1 cut(s) 374
Psp6I CCWGG 1 cut(s) 72
PspCI CACGTG 1 cut(s) 935
PspFI CCCAGC 2 cut(s) 967, 979
PspGI CCWGG 1 cut(s) 72
PspN4I GGNNCC 3 cut(s) 964, 1811, 2098
PspPI GGNCC 3 cut(s) 497, 2097, 2151
PstI CTGCAG 1 cut(s) 2237
PstNI CAGNNNCTG 2 cut(s) 43, 986
PsuI RGATCY 3 cut(s) 69, 196, 681
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 5 cut(s) 318, 1233, 1552, 1743, 1811
RsaNI GTAC 5 cut(s) 317, 1232, 1551, 1742, 1810
RseI CAYNNNNRTG 3 cut(s) 2091, 2706, 2748
SacI GAGCTC 1 cut(s) 374
SalI GTCGAC 1 cut(s) 780
SaqAI TTAA 7 cut(s) 56, 1083, 1331, 1832, 1883, 2436, 2757
Sau3AI GATC 8 cut(s) 69, 196, 241, 559, 673, 681, 1816, 2127
Sau96I GGNCC 3 cut(s) 497, 2097, 2151
ScaI AGTACT 1 cut(s) 1233
SchI GAGTC 7 cut(s) 606, 1561, 1687, 1859, 2005, 2228, 2802
ScrFI CCNGG 1 cut(s) 74
SduI GDGCHC 5 cut(s) 374, 854, 967, 2232, 2745
SfaNI GCATC 4 cut(s) 151, 2066, 2095, 2588
SfcI CTRYAG 1 cut(s) 2233
Sfr274I CTCGAG 1 cut(s) 63
SfuI TTCGAA 1 cut(s) 2292
SinI GGWCC 2 cut(s) 497, 2097
SlaI CTCGAG 1 cut(s) 63
SmiMI CAYNNNNRTG 3 cut(s) 2091, 2706, 2748
SmlI CTYRAG 3 cut(s) 63, 677, 1082
SmoI CTYRAG 3 cut(s) 63, 677, 1082
SphI GCATGC 1 cut(s) 1864
SsiI CCGC 9 cut(s) 26, 138, 149, 322, 397, 632, 1645, 1738, 1807
SspI AATATT 2 cut(s) 1409, 1887
SspMI CTAG 2 cut(s) 2261, 2595
SstI GAGCTC 1 cut(s) 374
StyD4I CCNGG 1 cut(s) 72
StyI CCWWGG 1 cut(s) 2107
TaaI ACNGT 4 cut(s) 669, 721, 1460, 2026
TaiI ACGT 4 cut(s) 937, 1153, 1883, 1951
TaqII GACCGA 2 cut(s) 1419, 1784
TatI WGTACW 1 cut(s) 1231
TauI GCSGC 2 cut(s) 29, 324
TfiI GAWTC 4 cut(s) 866, 1781, 2370, 2636
Tru1I TTAA 7 cut(s) 56, 1083, 1331, 1832, 1883, 2436, 2757
Tru9I TTAA 7 cut(s) 56, 1083, 1331, 1832, 1883, 2436, 2757
TscAI CASTG 4 cut(s) 198, 699, 991, 2490
TseFI GTSAC 3 cut(s) 721, 745, 925
Tsp45I GTSAC 3 cut(s) 721, 745, 925
TspGWI ACGGA 1 cut(s) 1774
TspRI CASTG 4 cut(s) 198, 699, 991, 2490
Vha464I CTTAAG 1 cut(s) 1082
VneI GTGCAC 1 cut(s) 2741
VpaK11BI GGWCC 2 cut(s) 497, 2097
VspI ATTAAT 2 cut(s) 1331, 1832
XagI CCTNNNNNAGG 2 cut(s) 895, 2579
XapI RAATTY 4 cut(s) 1069, 1313, 2294, 2556
XbaI TCTAGA 2 cut(s) 2260, 2594
XceI RCATGY 6 cut(s) 858, 1049, 1557, 1860, 1864, 2342
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XhoI CTCGAG 1 cut(s) 63
XmiI GTMKAC 1 cut(s) 781
XmnI GAANNNNTTC 1 cut(s) 2556
XspI CTAG 2 cut(s) 2261, 2595
ZrmI AGTACT 1 cut(s) 1233
Zsp2I ATGCAT 2 cut(s) 1479, 1862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.