Prupe.2G022900_v2.0.a1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000070
Physical Location & Seq
Reverse (-)
2219719 .. 2223944
4226 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G022900.1

Sequence Viewer

Length: 3846 bp
ATGGGGGAGGCTTATCTTGTGGCATTGCTTCAAGTGCTGGTTGACAAGTTGGCGCATCGTGAGGTGTTCAAGTACTTTGGACTCATAAAGGGCGTCGACCAAAAGCTGCAGAAATGGACTGCCACCTTGTCTGCAATTGGAGCTATTCTGAATGACGCGGAGGAGAGGCAACTAATTACCGAGAGCAAGCCGCTGAAGCTTTGGCTGGATGATCTCAGGTACTTAGCTTATGACGTGGAAGACGTATTGGACAAATATGCTACTAAAATGCTCAAACGGGAGATAGAGCTAGGACATTATGCCGGCACCGCAAGAAGGGTCTGGAACTCAGTTCCTAATGGTGTTTTCAACTACAAAATGAACTCAGAAATACAGAAGATTACTGAGCGTTTACAAGAGATATCTCACCGAAAGGACCAGCTTAGTTTGAATATCATTACTGGGATGACGTCGTCCACTAAGGCACGTCAAAATTTACCTCCCAGTTCCAGTCAACCAGATGGACCTGTGATTGGAAGGGACGAAGACAAAAGGCAGGTCGTGGAATTTCTGTCGAAACAAGAGCGTGGTGCCGTCAATTTCGATGTAGTTGCCATTGTTGGTATGGCTGGAGTCGGAAAGACAACACTTGCTGCACAAGTGTTCAATGAAATTGATGCAACCCAGCAATTTGAACCGGCTGCTTGGGTATGCGTGTCTGATGACTTCAACCTAGAAAGAGTGACAAAGAAAATTTTCGAATCAGTCACATCCCGGCACTGTCCTACAGAAGATTTCAATCAGGTTCAACAGTATCTGCATAAGGAATTAGCTGGGAAGAAGTTTTTTATTGTTTTGGATGATGTTTGGGGCACATGTAGCTATGGTCTATGGATGAAACTGCAGTCCCCATTTCGCGATGGAGCAGCAGGAAGCAAGATAATTGTGACAACGCGTGACGCAGAAGTTTCGAAAATGATGGGAGCTGGCACTCTAGTTCATAATTTGGAGCCTATGTCAAATGACGTTTGTTTTGAAGTATTTGAGCAGCATGCATTCAGGAATGTTAACAGAGATATACCACCAAATTTCGAGTCACTCAAGGAGAAAATTGTTGCAAGATGCAGTGGATTGCCTTTGGCTGCTAGGACTCTTGGCGGCCTTTTACTTCGTAAAGAGATGAATGAATGGGAGGAAATATTGAACAACAAATTATGGAGTCTATCAAATGAGTGTGACATACTTCCGGTATTAAGATTAAGCTATCACTATCTTCCTTCACATTTGAAAAGATGCTTTGCCTATTGTTCGATACTTCCAAATGACTATCAATTTAGTGAGCAGCAATTGATCCTTCTGTGGATGGCAGAGGGTTTGATTCAACCACAACCAGAACATAATAAGCAAATGGAAGATTTAGGAACTGACTATTTTCAGGAGCTCTTATCGAGGTCATTATTTCAGAAATCAAGCAAAAACAATTCAAAATATGTAATGCATGACCTCATTGTTGGTTTGGCACAGTGGGCAGCAGGAGATATTTGTTTTAGATGGGAGGATAAGCAGAATAGCGATCATGTACAACTTGGATGTTTTCCAAAGGCCCGCCATGCGTCCTACATTTGTGGTGACTATGACTATGATGTGGTTAAAAGATTTGAGGCATTTTCTGAAGTGAAACATTTGCGGACCTTCCTGCCTCTTTCTCATGGTTATCCTTGCCATTGTCTAAGTCGTACGGATCCTTTTGTTTTGTTGCCAAAATTGCAATACTTGCGGGTGCTTTCTCTCAATGGCGATAATGTAACAACCCTCTATCTCATCAATATTGGTAAATTGAAGTATCTGCGGTATCTTGATCTCTCTCACACACAGATAACAAGTTTGCCTAAATCAACTACCTCTCTTTACAACTTACAGACATTGTTATTGGAAGGTTGTCATTATTTGAAGGCACTACCTAGAAACTTAGGGAATCTAGTGAATCTGCGTCATCTCAATAACTCAGATGTAGGTTCACTGAAAGCAATGCCTCCGCAACTAGGTCGGTTGACGAATCTACGAGCTTTGTCTAATTTTGTGGTGGGTAAAGGTAGTGATCAATCGGGTATAAGAGAGATAGGGTCCCTATTCCATCTCCGAGGGACATTGCGCCTCTCAAGATTAGAGAATGTGATTGATGCTGAGGATGCACGGAGGGCCAACTTAAAATGCAAGGACAGACTTGATGAATTGGTGCTAGAATGGTCGACTCGTTTATTCTCTACTATGGTTGATGTAAGGTTAAACAACTGTGAGAAGTGCAAAATCTTGCCACCACTTGGACAATTGGCTTCACTCAAAATGCTTTATATTAGAGGAATGACTGCAGTGGAAAATGTTGGTCCTGAGTTTTATGGAGAGAGTAGCTTGCCTTTTCCAGTACTAGAGGATCTTAAGTTTTCAGATATGCACAACTGGAAGAAATGGCTTCCTTTCGCACAAGATCAGGTTTTCCCTTGCTTGAAGTTGCTTTCTATCACAAATTGCCCTCAATTGGAGGGTAAGTTGCCCGAGAACCTTGATTCATTAGCAACACTTGAAATTATTAAATGCGAGGAATTGGTGATTTCAATTTCCAACTATAAACAAATTGGTGCATTAAAAATCGACAGTTGCAAAGCGGTGGTGAAGACAAGTGGCGTTGAGTTTGAGTTATTAAATTCCTTGGAACTTTCAAATATTTCAGAGCTGAGGTTCCAAACAGGGGAGTTCACCAAAGGATTAAGGAAGCTGGGAAAAGAAGCAGAGGAGTTGCTGCAATTGCAAATATTGACTTGCAAGCTTAAATATCTAGAATTAAACAAGTGTGCAAGTCTTTCGAAGGTACCAGAAGGGTTGCATCACCTAACGGCTCTTCAAGACCTTGAAATAGTTGGATGCTCAAGTCTGGTTTCTTTTCCAGATGTTGGTCTGCCTCCTTCCCTTGAAGTCATAAGCATTCGGATGTGTGATTTGTTGCTGTATTTCGCAAAATACCAGATTCCCCCAAATCTAAGAAGAATAGAGATAAGGAGGTGTAAAAGTTTGAAATCATTAGTAGAGAAAGAGGAGGATAGTTCTTCGTCTTCCTCTTCTTCTCATATTTCTCTTGAGCACTTGGAAATAGGGGGATGTGAATCTCTAACGTCGTTATCATTGAGAGCCCAGTTGTTTCCCAGGGCGCTTAAATGCCTTGACATATCCGATTGTGGAGAGCTGCAGTTAATAACATCCGATGGGTTCTCCCACGACAACACTAATTATTCCGCCTCCAGCCTGAGACAAATCAACATCGAAAATTGCAACAAATTGGACATGTTACCGGACATGCTCAATCTCAATTGTCTTCAGCAATTTTCTATTGATTACGGTGAAGGTTTAGATTTCACTTCCTTTCCCCCCAACCTAACATCACTTGAAATCAGCGGAATCAAGAATTGTAAGCCACTGTGGGAGTTGTTGCACAGGCTCACCTCTCTTACATACTTGGGGGTCGATGGCGAAGACCCATATGTTGTGTCGTTTCCACCCGAAGGTGATACGGATATGGATATGGAGATGCTGCTCCCCGAATCTCTCACTCATCTCTCAATTGGGGGCTTCCCAAATCTGAAGAAACTGAGCAGCAAGGCCTTTCAATTCCTCACCTCTCTTCAATATCTTCGACTGCAGAATTGTCAAAAGCTGGCATCCATTCCAGAGGAGGGGCCTATTTCACTGAGGGAACTTCATATCATTAGGTGTCCTCTGCTAAAAGATAAATGCCCTAATTATATCATTGGGTTTGAAATTGAAGGACGATACCTGCCCAATTTATCCCACATCCCCCGCATACATATAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1282

Amino Acids

145.09

Weight (kDa)

6.22

Isoelectric Point (pI)

48.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 452
Acc36I ACCTGC 2 cut(s) 526, 3813
Acc65I GGTACC 1 cut(s) 2845
AccB1I GGYRCC 3 cut(s) 305, 569, 2845
AccB7I CCANNNNNTGG 2 cut(s) 2300, 2927
AccI GTMKAC 2 cut(s) 96, 2228
AccII CGCG 3 cut(s) 158, 897, 934
AclWI GGATC 4 cut(s) 1324, 1715, 1728, 2418
AcsI RAATTY 5 cut(s) 472, 545, 732, 1066, 2680
AcuI CTGAAG 4 cut(s) 215, 1671, 3332, 3632
AcyI GRCGYC 2 cut(s) 93, 449
AfaI GTAC 6 cut(s) 74, 221, 1560, 1717, 2403, 2847
AfiI CCNNNNNNNGG 9 cut(s) 315, 512, 2021, 2300, 2515, 2725, 2927, 3533, 3704
AflII CTTAAG 1 cut(s) 2414
AflIII ACRYGT 3 cut(s) 854, 932, 3315
AjiI CACGTC 2 cut(s) 235, 467
AjnI CCWGG 1 cut(s) 3176
Alw21I GWGCWC 2 cut(s) 1422, 3117
Alw26I GTCTC 1 cut(s) 3274
AlwI GGATC 4 cut(s) 1324, 1715, 1728, 2418
AlwNI CAGNNNCTG 1 cut(s) 796
Ama87I CYCGRG 1 cut(s) 2531
AoxI GGCC 5 cut(s) 1138, 1581, 2178, 3630, 3707
ApoI RAATTY 5 cut(s) 472, 545, 732, 1066, 2680
ArsI GACNNNNNNTTYG 6 cut(s) 522, 554, 995, 1027, 1058, 1090
Asp700I GAANNNNTTC 1 cut(s) 734
Asp718I GGTACC 1 cut(s) 2845
AspLEI GCGC 3 cut(s) 55, 2133, 3184
AspS9I GGNCC 8 cut(s) 415, 503, 1582, 1668, 2103, 2178, 2363, 3707
AsuC2I CCSGG 1 cut(s) 754
AsuII TTCGAA 3 cut(s) 738, 950, 2840
AvaI CYCGRG 1 cut(s) 2531
AvaII GGWCC 5 cut(s) 415, 503, 1668, 2103, 2363
BaeGI GKGCMC 1 cut(s) 854
BamHI GGATCC 1 cut(s) 1720
BanI GGYRCC 3 cut(s) 305, 569, 2845
BanII GRGCYC 2 cut(s) 1422, 3166
BbsI GAAGAC 6 cut(s) 246, 531, 2657, 3078, 3338, 3510
Bbv12I GWGCWC 2 cut(s) 1422, 3117
BbvCI CCTCAGC 2 cut(s) 2163, 2711
BccI CCATC 8 cut(s) 494, 893, 952, 1336, 1524, 2121, 3230, 3491
BceAI ACGGC 2 cut(s) 557, 2886
BcgI CGANNNNNNTGC 8 cut(s) 572, 606, 930, 964, 2006, 2040, 2820, 2854
BciT130I CCWGG 1 cut(s) 3178
BclI TGATCA 1 cut(s) 2077
BcnI CCSGG 1 cut(s) 754
BcoDI GTCTC 1 cut(s) 3274
BfmI CTRYAG 6 cut(s) 107, 765, 881, 2346, 3218, 3668
BfoI RGCGCY 1 cut(s) 3185
BfrI CTTAAG 1 cut(s) 2414
BfuAI ACCTGC 2 cut(s) 526, 3813
BmcAI AGTACT 2 cut(s) 74, 2403
Bme1390I CCNGG 2 cut(s) 754, 3178
Bme18I GGWCC 5 cut(s) 415, 503, 1668, 2103, 2363
BmeT110I CYCGRG 1 cut(s) 2531
BmgBI CACGTC 2 cut(s) 235, 467
BmgT120I GGNCC 8 cut(s) 415, 503, 1582, 1668, 2103, 2178, 2363, 3707
BmiI GGNNCC 9 cut(s) 307, 571, 990, 1722, 2104, 2105, 2717, 2847, 3708
BmrFI CCNGG 2 cut(s) 754, 3178
BmrI ACTGGG 3 cut(s) 450, 477, 3160
BmuI ACTGGG 3 cut(s) 450, 477, 3160
BpiI GAAGAC 6 cut(s) 246, 531, 2657, 3078, 3338, 3510
BpmI CTGGAG 2 cut(s) 630, 3256
Bpu10I CCTNAGC 2 cut(s) 2163, 2711
Bpu14I TTCGAA 3 cut(s) 738, 950, 2840
BpuEI CTTGAG 4 cut(s) 1064, 2122, 2887, 3131
BpuMI CCSGG 1 cut(s) 754
BsaBI GATNNNNATC 2 cut(s) 777, 3136
BsaHI GRCGYC 2 cut(s) 93, 449
BsaJI CCNNGG 4 cut(s) 2119, 2685, 3176, 3177
BsaWI WCCGGW 2 cut(s) 1225, 3322
BsaXI ACNNNNNCTCC 4 cut(s) 980, 1010, 2510, 2540
Bsc4I CCNNNNNNNGG 9 cut(s) 315, 512, 2021, 2300, 2515, 2725, 2927, 3533, 3704
Bse118I RCCGGY 2 cut(s) 302, 676
Bse1I ACTGG 6 cut(s) 445, 483, 489, 2399, 2441, 3166
Bse3DI GCAATG 3 cut(s) 23, 2013, 2126
Bse8I GATNNNNATC 2 cut(s) 777, 3136
BseBI CCWGG 1 cut(s) 3178
BseDI CCNNGG 4 cut(s) 2119, 2685, 3176, 3177
BseJI GATNNNNATC 2 cut(s) 777, 3136
BseLI CCNNNNNNNGG 9 cut(s) 315, 512, 2021, 2300, 2515, 2725, 2927, 3533, 3704
BseMI GCAATG 3 cut(s) 23, 2013, 2126
BseNI ACTGG 6 cut(s) 445, 483, 489, 2399, 2441, 3166
BseRI GAGGAG 4 cut(s) 176, 2783, 3083, 3716
BseSI GKGCMC 1 cut(s) 854
BseYI CCCAGC 3 cut(s) 663, 812, 2752
BsgI GTGCAG 1 cut(s) 618
Bsh1236I CGCG 3 cut(s) 158, 897, 934
BshFI GGCC 5 cut(s) 1140, 1583, 2180, 3632, 3709
BshNI GGYRCC 3 cut(s) 305, 569, 2845
BsiHKAI GWGCWC 2 cut(s) 1422, 3117
BsiHKCI CYCGRG 1 cut(s) 2531
BsiSI CCGG 5 cut(s) 303, 677, 754, 1226, 3323
BsiWI CGTACG 1 cut(s) 1715
BslFI GGGAC 4 cut(s) 533, 871, 2089, 2137
BslI CCNNNNNNNGG 9 cut(s) 315, 512, 2021, 2300, 2515, 2725, 2927, 3533, 3704
BsmAI GTCTC 1 cut(s) 3274
BsmFI GGGAC 4 cut(s) 533, 871, 2089, 2137
BsmI GAATGC 2 cut(s) 1034, 2958
BsnI GGCC 5 cut(s) 1140, 1583, 2180, 3632, 3709
BsoBI CYCGRG 1 cut(s) 2531
Bsp119I TTCGAA 3 cut(s) 738, 950, 2840
Bsp1286I GDGCHC 4 cut(s) 854, 1422, 3117, 3166
Bsp1407I TGTACA 1 cut(s) 1558
Bsp143I GATC 8 cut(s) 211, 1329, 1552, 1720, 1837, 2077, 2410, 2464
Bsp68I TCGCGA 1 cut(s) 897
BspANI GGCC 5 cut(s) 1140, 1583, 2180, 3632, 3709
BspFNI CGCG 3 cut(s) 158, 897, 934
BspLI GGNNCC 9 cut(s) 307, 571, 990, 1722, 2104, 2105, 2717, 2847, 3708
BspMAI CTGCAG 5 cut(s) 111, 885, 2350, 3222, 3672
BspMI ACCTGC 2 cut(s) 526, 3813
BspPI GGATC 4 cut(s) 1324, 1715, 1728, 2418
BspQI GCTCTTC 1 cut(s) 2880
BspT104I TTCGAA 3 cut(s) 738, 950, 2840
BspT107I GGYRCC 3 cut(s) 305, 569, 2845
BspTI CTTAAG 1 cut(s) 2414
BsrDI GCAATG 3 cut(s) 23, 2013, 2126
BsrFI RCCGGY 2 cut(s) 302, 676
BsrGI TGTACA 1 cut(s) 1558
BsrI ACTGG 6 cut(s) 445, 483, 489, 2399, 2441, 3166
BssAI RCCGGY 2 cut(s) 302, 676
BssECI CCNNGG 4 cut(s) 2119, 2685, 3176, 3177
BssMI GATC 8 cut(s) 211, 1329, 1552, 1720, 1837, 2077, 2410, 2464
BssNI GRCGYC 2 cut(s) 93, 449
BssT1I CCWWGG 1 cut(s) 2685
Bst2UI CCWGG 1 cut(s) 3178
Bst4CI ACNGT 7 cut(s) 761, 792, 1503, 2273, 2633, 3371, 3450
Bst6I CTCTTC 3 cut(s) 2880, 3097, 3657
BstACI GRCGYC 2 cut(s) 93, 449
BstAFI CTTAAG 1 cut(s) 2414
BstAPI GCANNNNNTGC 1 cut(s) 1753
BstAUI TGTACA 1 cut(s) 1558
BstBI TTCGAA 3 cut(s) 738, 950, 2840
BstC8I GCNNGC 8 cut(s) 188, 304, 967, 1032, 1585, 2390, 2801, 3687
BstFNI CGCG 3 cut(s) 158, 897, 934
BstH2I RGCGCY 1 cut(s) 3185
BstHHI GCGC 3 cut(s) 55, 2133, 3184
BstKTI GATC 8 cut(s) 214, 1332, 1555, 1723, 1840, 2080, 2413, 2467
BstMAI GTCTC 1 cut(s) 3274
BstMBI GATC 8 cut(s) 211, 1329, 1552, 1720, 1837, 2077, 2410, 2464
BstNI CCWGG 1 cut(s) 3178
BstNSI RCATGY 4 cut(s) 858, 1034, 3319, 3331
BstSCI CCNGG 2 cut(s) 752, 3176
BstSFI CTRYAG 6 cut(s) 107, 765, 881, 2346, 3218, 3668
BstSLI GKGCMC 1 cut(s) 854
BstUI CGCG 3 cut(s) 158, 897, 934
BstV2I GAAGAC 6 cut(s) 246, 531, 2657, 3078, 3338, 3510
BstX2I RGATCY 2 cut(s) 1720, 2410
BstYI RGATCY 2 cut(s) 1720, 2410
BsuRI GGCC 5 cut(s) 1140, 1583, 2180, 3632, 3709
BtgZI GCGATG 1 cut(s) 912
BtrI CACGTC 2 cut(s) 235, 467
BtsI GCAGTG 2 cut(s) 1111, 2355
BtsIMutI CAGTG 7 cut(s) 757, 1111, 1508, 1997, 2355, 3446, 3716
BtuMI TCGCGA 1 cut(s) 897
BveI ACCTGC 2 cut(s) 526, 3813
Cac8I GCNNGC 8 cut(s) 188, 304, 967, 1032, 1585, 2390, 2801, 3687
CaiI CAGNNNCTG 1 cut(s) 796
CfoI GCGC 3 cut(s) 55, 2133, 3184
Cfr10I RCCGGY 2 cut(s) 302, 676
Cfr13I GGNCC 8 cut(s) 415, 503, 1582, 1668, 2103, 2178, 2363, 3707
CseI GACGC 5 cut(s) 82, 164, 947, 1581, 1958
Csp6I GTAC 6 cut(s) 73, 220, 1559, 1716, 2402, 2846
CviAII CATG 8 cut(s) 855, 1031, 1478, 1556, 1589, 1688, 3316, 3328
CviQI GTAC 6 cut(s) 73, 220, 1559, 1716, 2402, 2846
DpnI GATC 8 cut(s) 213, 1331, 1554, 1722, 1839, 2079, 2412, 2466
DpnII GATC 8 cut(s) 211, 1329, 1552, 1720, 1837, 2077, 2410, 2464
Eam1104I CTCTTC 3 cut(s) 2880, 3097, 3657
EarI CTCTTC 3 cut(s) 2880, 3097, 3657
EciI GGCGGA 1 cut(s) 3256
Ecl136II GAGCTC 1 cut(s) 1420
Eco130I CCWWGG 1 cut(s) 2685
Eco147I AGGCCT 1 cut(s) 3632
Eco24I GRGCYC 2 cut(s) 1422, 3166
Eco32I GATATC 1 cut(s) 402
Eco47I GGWCC 5 cut(s) 415, 503, 1668, 2103, 2363
Eco53kI GAGCTC 1 cut(s) 1420
Eco57I CTGAAG 4 cut(s) 215, 1671, 3332, 3632
Eco88I CYCGRG 1 cut(s) 2531
EcoICRI GAGCTC 1 cut(s) 1420
EcoO109I RGGNCCY 2 cut(s) 2103, 3707
EcoRII CCWGG 1 cut(s) 3176
EcoRV GATATC 1 cut(s) 402
EcoT14I CCWWGG 1 cut(s) 2685
EcoT22I ATGCAT 2 cut(s) 1036, 1479
EcoT38I GRGCYC 2 cut(s) 1422, 3166
ErhI CCWWGG 1 cut(s) 2685
FaeI CATG 8 cut(s) 858, 1034, 1481, 1559, 1592, 1691, 3319, 3331
FaqI GGGAC 4 cut(s) 533, 871, 2089, 2137
FatI CATG 8 cut(s) 854, 1030, 1477, 1555, 1588, 1687, 3315, 3327
FauI CCCGC 3 cut(s) 1592, 1749, 3836
FauNDI CATATG 1 cut(s) 3511
FbaI TGATCA 1 cut(s) 2077
FblI GTMKAC 2 cut(s) 96, 2228
FriOI GRGCYC 2 cut(s) 1422, 3166
GlaI GCGC 3 cut(s) 54, 2132, 3183
GsaI CCCAGC 3 cut(s) 667, 816, 2756
GsuI CTGGAG 2 cut(s) 630, 3256
HaeII RGCGCY 1 cut(s) 3185
HaeIII GGCC 5 cut(s) 1140, 1583, 2180, 3632, 3709
HapII CCGG 5 cut(s) 303, 677, 754, 1226, 3323
HgaI GACGC 5 cut(s) 82, 164, 947, 1581, 1958
HhaI GCGC 3 cut(s) 55, 2133, 3184
Hin1I GRCGYC 2 cut(s) 93, 449
Hin1II CATG 8 cut(s) 858, 1034, 1481, 1559, 1592, 1691, 3319, 3331
Hin6I GCGC 3 cut(s) 53, 2131, 3182
HinP1I GCGC 3 cut(s) 53, 2131, 3182
HincII GTYRAC 6 cut(s) 43, 97, 494, 1048, 2031, 2229
HindII GTYRAC 6 cut(s) 43, 97, 494, 1048, 2031, 2229
HindIII AAGCTT 2 cut(s) 197, 2801
HpaI GTTAAC 1 cut(s) 1048
HpaII CCGG 5 cut(s) 303, 677, 754, 1226, 3323
Hpy99I CGWCG 3 cut(s) 98, 454, 3151
HpyCH4III ACNGT 7 cut(s) 761, 792, 1503, 2273, 2633, 3371, 3450
HpyCH4IV ACGT 6 cut(s) 234, 243, 449, 466, 1005, 3146
HpySE526I ACGT 6 cut(s) 234, 243, 449, 466, 1005, 3146
Hsp92I GRCGYC 2 cut(s) 93, 449
Hsp92II CATG 8 cut(s) 858, 1034, 1481, 1559, 1592, 1691, 3319, 3331
HspAI GCGC 3 cut(s) 53, 2131, 3182
KflI GGGWCCC 1 cut(s) 2103
KpnI GGTACC 1 cut(s) 2849
KroI GCCGGC 1 cut(s) 302
KroNI GCCGGC 1 cut(s) 304
Ksp22I TGATCA 1 cut(s) 2077
KspAI GTTAAC 1 cut(s) 1048
Kzo9I GATC 8 cut(s) 211, 1329, 1552, 1720, 1837, 2077, 2410, 2464
LguI GCTCTTC 1 cut(s) 2880
LmnI GCTCC 6 cut(s) 140, 902, 962, 988, 1417, 3570
MaeII ACGT 6 cut(s) 234, 243, 449, 466, 1005, 3146
MaeIII GTNAC 9 cut(s) 721, 745, 925, 935, 1074, 1214, 1607, 1783, 3318
MalI GATC 8 cut(s) 213, 1331, 1554, 1722, 1839, 2079, 2412, 2466
MboI GATC 8 cut(s) 211, 1329, 1552, 1720, 1837, 2077, 2410, 2464
MfeI CAATTG 7 cut(s) 135, 1325, 2306, 2513, 2780, 3340, 3591
MflI RGATCY 2 cut(s) 1720, 2410
MhlI GDGCHC 4 cut(s) 854, 1422, 3117, 3166
MluI ACGCGT 1 cut(s) 932
MlyI GAGTC 6 cut(s) 75, 621, 1082, 1123, 1207, 2224
MmeI TCCRAC 3 cut(s) 595, 2622, 2875
Mph1103I ATGCAT 2 cut(s) 1036, 1479
MroNI GCCGGC 1 cut(s) 302
MroXI GAANNNNTTC 1 cut(s) 734
MslI CAYNNNNRTG 1 cut(s) 2963
MspA1I CMGCKG 2 cut(s) 193, 3426
MspCI CTTAAG 1 cut(s) 2414
MspI CCGG 5 cut(s) 303, 677, 754, 1226, 3323
MspR9I CCNGG 2 cut(s) 754, 3178
MunI CAATTG 7 cut(s) 135, 1325, 2306, 2513, 2780, 3340, 3591
Mva1269I GAATGC 2 cut(s) 1034, 2958
MvaI CCWGG 1 cut(s) 3178
MvnI CGCG 3 cut(s) 158, 897, 934
NaeI GCCGGC 1 cut(s) 304
NciI CCSGG 1 cut(s) 754
NdeI CATATG 1 cut(s) 3511
NdeII GATC 8 cut(s) 211, 1329, 1552, 1720, 1837, 2077, 2410, 2464
NgoMIV GCCGGC 1 cut(s) 302
NlaIII CATG 8 cut(s) 858, 1034, 1481, 1559, 1592, 1691, 3319, 3331
NlaIV GGNNCC 9 cut(s) 307, 571, 990, 1722, 2104, 2105, 2717, 2847, 3708
NmuCI GTSAC 7 cut(s) 721, 745, 925, 935, 1074, 1214, 1607
NruI TCGCGA 1 cut(s) 897
NsiI ATGCAT 2 cut(s) 1036, 1479
NspI RCATGY 4 cut(s) 858, 1034, 3319, 3331
NspV TTCGAA 3 cut(s) 738, 950, 2840
PaeI GCATGC 1 cut(s) 1034
PasI CCCWGGG 1 cut(s) 3177
PceI AGGCCT 1 cut(s) 3632
PciI ACATGT 2 cut(s) 854, 3315
PciSI GCTCTTC 1 cut(s) 2880
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PctI GAATGC 2 cut(s) 1034, 2958
PdiI GCCGGC 1 cut(s) 304
PdmI GAANNNNTTC 1 cut(s) 734
Pfl23II CGTACG 1 cut(s) 1715
PflFI GACNNNGTC 1 cut(s) 451
PflMI CCANNNNNTGG 2 cut(s) 2300, 2927
PleI GAGTC 6 cut(s) 75, 620, 1081, 1123, 1206, 2224
PpsI GAGTC 6 cut(s) 75, 620, 1081, 1123, 1206, 2224
PpuMI RGGWCCY 1 cut(s) 2103
PscI ACATGT 2 cut(s) 854, 3315
Psp124BI GAGCTC 1 cut(s) 1422
Psp5II RGGWCCY 1 cut(s) 2103
Psp6I CCWGG 1 cut(s) 3176
PspFI CCCAGC 3 cut(s) 663, 812, 2752
PspGI CCWGG 1 cut(s) 3176
PspLI CGTACG 1 cut(s) 1715
PspN4I GGNNCC 9 cut(s) 307, 571, 990, 1722, 2104, 2105, 2717, 2847, 3708
PspPI GGNCC 8 cut(s) 415, 503, 1582, 1668, 2103, 2178, 2363, 3707
PspPPI RGGWCCY 1 cut(s) 2103
PstI CTGCAG 5 cut(s) 111, 885, 2350, 3222, 3672
PstNI CAGNNNCTG 1 cut(s) 796
PsuI RGATCY 2 cut(s) 1720, 2410
PsyI GACNNNGTC 1 cut(s) 451
RruI TCGCGA 1 cut(s) 897
RsaI GTAC 6 cut(s) 74, 221, 1560, 1717, 2403, 2847
RsaNI GTAC 6 cut(s) 73, 220, 1559, 1716, 2402, 2846
RseI CAYNNNNRTG 1 cut(s) 2963
SacI GAGCTC 1 cut(s) 1422
SalI GTCGAC 2 cut(s) 95, 2227
SapI GCTCTTC 1 cut(s) 2880
Sau3AI GATC 8 cut(s) 211, 1329, 1552, 1720, 1837, 2077, 2410, 2464
Sau96I GGNCC 8 cut(s) 415, 503, 1582, 1668, 2103, 2178, 2363, 3707
ScaI AGTACT 2 cut(s) 74, 2403
SchI GAGTC 6 cut(s) 75, 621, 1082, 1123, 1207, 2224
ScrFI CCNGG 2 cut(s) 754, 3178
SduI GDGCHC 4 cut(s) 854, 1422, 3117, 3166
SfcI CTRYAG 6 cut(s) 107, 765, 881, 2346, 3218, 3668
SfuI TTCGAA 3 cut(s) 738, 950, 2840
SinI GGWCC 5 cut(s) 415, 503, 1668, 2103, 2363
SmiMI CAYNNNNRTG 1 cut(s) 2963
SmlI CTYRAG 5 cut(s) 1079, 2137, 2414, 2902, 3110
SmoI CTYRAG 5 cut(s) 1079, 2137, 2414, 2902, 3110
SphI GCATGC 1 cut(s) 1034
SseBI AGGCCT 1 cut(s) 3632
SspI AATATT 4 cut(s) 1179, 1807, 2701, 2790
SstI GAGCTC 1 cut(s) 1422
StuI AGGCCT 1 cut(s) 3632
StyD4I CCNGG 2 cut(s) 752, 3176
StyI CCWWGG 1 cut(s) 2685
TaaI ACNGT 7 cut(s) 761, 792, 1503, 2273, 2633, 3371, 3450
TaiI ACGT 6 cut(s) 237, 246, 452, 469, 1008, 3149
TatI WGTACW 3 cut(s) 72, 1558, 2401
TauI GCSGC 2 cut(s) 193, 1140
TscAI CASTG 7 cut(s) 764, 1111, 1508, 2004, 2355, 3453, 3723
TseFI GTSAC 7 cut(s) 721, 745, 925, 935, 1074, 1214, 1607
Tsp45I GTSAC 7 cut(s) 721, 745, 925, 935, 1074, 1214, 1607
TspDTI ATGAA 9 cut(s) 374, 663, 890, 968, 1175, 1179, 2223, 2535, 3719
TspGWI ACGGA 3 cut(s) 1733, 2188, 3557
TspRI CASTG 7 cut(s) 764, 1111, 1508, 2004, 2355, 3453, 3723
Tth111I GACNNNGTC 1 cut(s) 451
Van91I CCANNNNNTGG 2 cut(s) 2300, 2927
Vha464I CTTAAG 1 cut(s) 2414
VpaK11BI GGWCC 5 cut(s) 415, 503, 1668, 2103, 2363
XapI RAATTY 5 cut(s) 472, 545, 732, 1066, 2680
XbaI TCTAGA 1 cut(s) 2812
XceI RCATGY 4 cut(s) 858, 1034, 3319, 3331
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XmiI GTMKAC 2 cut(s) 96, 2228
XmnI GAANNNNTTC 1 cut(s) 734
ZraI GACGTC 1 cut(s) 450
ZrmI AGTACT 2 cut(s) 74, 2403
Zsp2I ATGCAT 2 cut(s) 1036, 1479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.