Rroxscaffold_160G00433990

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000160
OG0000070
Physical Location & Seq
Forward (+)
487378 .. 491448
4071 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_160G00433990.1

Sequence Viewer

Length: 4071 bp
ATGGTAGCGGAGGTCTTCCTCGGTGCATTCCTTCAGTTACTGCTCGACAGGTTAACTCCTCATCACGATTTGCTCAACTTGGCGTGCCTGCATGGCGTCGACAAGAAACTCAAGAAATGGAGTGCAGCGCTGTCTGCAATCGGAGCGGTGCTGCAGGACGCGGAGGACAAGCAACTGACGAGCGAGGCTGTGAAGCTATGGCTGGATGAACTCAAACACTTGGCTTTTGACATGGATGACATCTTGGACACCTTTTCCACCGAGCTGTTGCGGCGCAACGTGAAGAATCAGCAACATGCGTGGCTTACGAGTAAGGTACGGGGAGCTAAGTTCAATTTCAATATGAATTCGGAGATCAAGGAGATTAGTGATAGGTTGGTAGAGATATCTGAAAGAAAGGACTTGCTTGGGTTGAATTATGTTGTGAATAAATGTAGTCAGGTGTGGCAAAGGCCGCCGAGTTCATGTGTGTTAGATGGGCCTGTGGTTGGTAGGGATGATGATAAAAGGAAGATTGTTGAGGTGTTATTGAGGGATGATCAGCCTAGTAGTGTCAATTTCCATGTGGTTGCCATTGTGGGTATGCCAGGACTTGGAAAGACTACAATTGCAGGTCATGTCTTCAATGATAATGCAATGGAACGGTTTAGTCCCAAAGTATGGGTATCTGTGTCTGATAACTTCAATCTTGTGAGAGTGACAAAGGCAATTCTGGAATCAGTTACATCTGCACGCTGTGATTTGGAGGAGTTCAGTCAAATTCAGGAGAGTCTGAGCAAGGCATTGGCAAGTAAAAAGTTTTTGGTTGTTTTAGATGATGTCTGGAACACATGTGACTATGATCTATGGGCAAAGCTGCAGTCTGCCTTTCGTGGCGGAGCATTGGGAAGTAAGGTAATTGTGACAACTCGCGACACACAAGTTGCTAAGATGATGAGATCCATTGAAGTTCATAGCTTGGGGTGCATGTCAGATGATGATTGCTGGGAAGTCTTTGGGCAACATGCATTCTTGAATGTTGAGAATGGGAGACTGCAAAGCTTTGAGTTGTTTCGGGAGAAAGTTGTAGCAAAATGTGGTGGGTTGCCCTTGGCTGCAAGAGTCCTTGGTGGTCTTTTAGGCTGTAAAGAGCTAGACGAATGGGAAGAAATATTGAACAGTAAATTATGGAGTCTGTGTGATAACAGTGGCATTCTCCCAGTATTAAAATTGAGCTATCACTATCTCTCTTCAAATTTGAAAAGGTGCTTTGCCTATTGCTCAATACTTCCAAATGACTATGAATTTGGGGAGAGGCAGTTGGTCCTTCTATGGATGGCAGAGGGTCTCATTCAGCAGTCACATGAATGTAAACAACTGGAAGATATAGGGAGTAACTATTTTCGGGAGCTATTGTCCAGGTCATTGTTTCAAAGGTCCAGTAAAAACCATTCACAATTTGTTATGCATGACCTTGTTGGTGATTTGTCACGGTGGGCTGCAGGAGACATGTGTTTCAGATTGGAGGATAAGCTGGATGTTAGATGTTCTTCAAAGGTTCGCCATTCCTCTTACATTCCTGGTCAATTTGATGGTGTTCAAAAATTCAAAGAATTTTTTGAAGTCAGGCATTTGCGGACTTTCTTACCACTTTCACTTTCAGGTCGATCAAATTATCTAACTCGTAATGTTACTTTTGATCTATTACCAAAGTTGAAGTACTTGAGGGCACTCTCTTTTAATGGCTATCAAATAACTGAGTTACCCAATTCTATTGGTAAGCTGAAGCATCTGCGGTACCTTGATCTTTCTCACACACAGATAACAACTTTGCCAGAATCAACCAGCAATCTGTACAACTTACAGACATTGATATTAGAAAATTGTGCTTGTTTGAAGGCACTGCCTGCAGACATGAGGAATCTAACTGATCTGCGGCACCTTAACAATTCAGATGTACCTTCATTGGAAGAAATGCCTGTGAAACTAGGTAGATTGACTAATCTTCAAACACTGAGTACTTTTGTGCTGGGCAAAGGTAGCAAGTCAGGAATAAGTGAGATAGGTCCTTTATTGTGTCTCCAAGGGACATTGTGCCTCTCAAGATTGGAGAATGTGACTGATGTTGATGATGCAAGGAGGGCTGACTTGACTAGCAAGGAAGGGATTGATTCATTGCAACTAGAATGGAGAGAATCAGGAGAAAGGGAATCAGATGTTTTTGAAATGTTAAAACCTCATAGGAAGCTCAAAGTGCTCACTGTAAAAGGTTATGGAGGTCTTACATTTTCAACATGGACTGGACACCCTTCATTCTCTTATATGACGCATGTAAAGTTGGAGCATTGTAAAAATTGTCGATTCTTGCCTCCACTTGGACAGTTGTCTTCTCTGAAAGAACTTTGTATAAAAGGAATGTCTACAATTGAAAGCGTGGGTCTTGAGTTTTATGGAGAGAGTAGCCTGCCTTTTCCTGTATTGGAGACCCTGGAGTTCAAGGATATGCAGAAATGGAGGGAGTGGCTTCCTCGGGTGCAAGATCAAGGAATTGAAGTTTTCCCTTCCTTGAAAATGGTTTGTATCTCTGGATGCCCCATACTGGAGGGGAGATTACCTGAAAACCTTGATCTATTATCGAAGCTTGCCATTGATGGATGCGGGCAATTGGTGGTTTCAATTGCCAGTTATAATCGTCTTCGTGATTTGAAAATTCATGCTTGCAAAATGCTGGTACATACAAGTGGGGTTCAATTTGAGTTACTAGAGGCGATGGAGCTTTCATGTATTTCAGAGTTTAGACTCCACATGGACGGATTCAAGAGAGACTTAACAAAGCTTAAAAATCTGAGAATTACTAGTTGTGAGGAGCTGACATCTTTATGGGAAAATGAGGATAGATTGCTGCAACGTCCGATTTATGAAGGAAACAGCTCTCACTCTTACTTTGTTCAAGAGCTGAAGTCACTTCAATGTCTTCACATAAATGATTGCTCAAATCTAGTTTCTTTTTCAAAGGTTAGCCTGCCACCTTCTCTTAAAGAAATAAAGATTGAGCGGTGCAGTTCCCTTACAAGATACCAGATACCCCCAAACGTAAGAAGTCTATACATAAATTCCTGCATAAACTTGGAATCATTAGTGGGGGAGGACAAGAAAGGTGAGGATACTTCATCTTCCTTTTCTCCTTGTTTGATACAAGAGGAGGCATCCTGCCTCGAGTACTTGAGCATTGTGAATTGTATGAGATTTACAACCTTATCATCCACAGGCCAGCTAGCCAGGGGACTTAAACATATTATCATAAGTCATTGTCACCAGCTAGAATCAATAACTGATAGCTTCCACGTTAACACCTTCCTTGAAGAAATTGTGATTAGGAGTTGTGGAAATCTTAAATCGTTACCAGAGGGCTTATGTGACCTCACCAATCTTCAAGTCTTTAAGATTTGGGACTGTGAAAGTCTTGTTTCTTTCCCAAGAGGAGGGTTGCCAACAAGAACCTCCAGCTTGAAAGAGATCGAGGTTATGCATTGTGATAACTTAGAGGCCCTTCCTAAAGACATGCAGAACCTCCACTGTCTTCAGAGTTTGAGGATTTCATATTGTGAAGGTTTCGCTCCATTTCTAGACAAGGGTTTGCCACCCAACCTAACATTCCTTGAGATTCGAAATCTCAACAGTTGTAGGCCACTCCTGGAAGCTCAAGGATTGCACAGACTCACTTCTCTTAGAGCGTTGGATATCAGGGGAAATGATTCACAACTAGTGTCATTTCCACCTGACAAGGAGATGGTGCTCCCCAAATCTCTCATTCTACTTCATATTGCAGGGTTCCCGAATCTGAGGCACCTATCCAGCAAGGGCTTTCAGTTCCTCCCCTCTCTTGAATCTCTTCAAGTCTTCAGCTGTCCAAAGCTAGCATCCATTCCTGAGGAGGGACTCCCTCTTTCACTTACACAACTTCATATCTTTGGTTGTCCACTGCTAGTAAAGAACTGCAGACCTGGAAAAGGACAATACTGGCACTCCATAGCTCGCATCCCTTTCATATGGATTAAGGGTGATAAGGGAGCAGACATATTTAGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1356

Amino Acids

153.25

Weight (kDa)

6.58

Isoelectric Point (pI)

46.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 7 - 99 1.1e-20 Rx N-terminal domain
NB-ARC PF00931 166 - 337 7.4e-37 NB-ARC domain
WHD_DRP PF23559 422 - 489 2.3e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 562 - 829 9.5e-23 Leucine-rich repeat region
LRR_8 PF13855 569 - 622 7.4e-06 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 678 - 799 3.4e-33 R13L1/DRL21 LRRs
LRR_14 PF23598 1123 - 1292 4.8e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2667
Acc36I ACCTGC 1 cut(s) 602
Acc65I GGTACC 1 cut(s) 1776
AccB1I GGYRCC 3 cut(s) 1776, 1917, 3825
AccB7I CCANNNNNTGG 2 cut(s) 593, 660
AccBSI CCGCTC 2 cut(s) 146, 3034
AccI GTMKAC 2 cut(s) 99, 2399
AccII CGCG 2 cut(s) 161, 912
AclWI GGATC 1 cut(s) 933
AcsI RAATTY 8 cut(s) 346, 759, 1234, 1283, 1583, 1592, 2688, 3091
AcuI CTGAAG 5 cut(s) 17, 1784, 2957, 3545, 3865
AcyI GRCGYC 1 cut(s) 96
AdeI CACNNNGTG 1 cut(s) 737
AfaI GTAC 8 cut(s) 318, 1700, 1778, 1835, 1938, 1999, 2712, 3200
AfeI AGCGCT 1 cut(s) 129
AfiI CCNNNNNNNGG 8 cut(s) 488, 593, 660, 2354, 2578, 3461, 3914, 3989
AflIII ACRYGT 2 cut(s) 830, 1488
AhlI ACTAGT 2 cut(s) 2834, 3742
AjnI CCWGG 7 cut(s) 586, 1397, 1558, 2466, 3257, 3672, 3982
AjuI GAANNNNNNNTTGG 2 cut(s) 3617, 3649
Alw21I GWGCWC 2 cut(s) 2238, 3777
Alw26I GTCTC 6 cut(s) 1024, 1331, 1479, 2063, 2456, 2796
AlwI GGATC 1 cut(s) 933
AlwNI CAGNNNCTG 1 cut(s) 40
Ama87I CYCGRG 2 cut(s) 2508, 3194
Aor51HI AGCGCT 1 cut(s) 129
AoxI GGCC 5 cut(s) 452, 479, 3247, 3525, 3665
ApeKI GCWGC 6 cut(s) 125, 151, 856, 1094, 1478, 2881
ApoI RAATTY 8 cut(s) 346, 759, 1234, 1283, 1583, 1592, 2688, 3091
ArsI GACNNNNNNTTYG 4 cut(s) 845, 877, 1268, 1300
Asp700I GAANNNNTTC 2 cut(s) 3733, 3870
Asp718I GGTACC 1 cut(s) 1776
AspLEI GCGC 2 cut(s) 130, 276
AspS9I GGNCC 5 cut(s) 479, 1303, 1416, 2045, 3526
AsuHPI GGTGA 5 cut(s) 1472, 3149, 3284, 3394, 4052
AsuII TTCGAA 1 cut(s) 3646
AsuNHI GCTAGC 2 cut(s) 3253, 3895
AvaI CYCGRG 2 cut(s) 2508, 3194
AvaII GGWCC 3 cut(s) 1303, 1416, 2045
AxyI CCTNAGG 1 cut(s) 3909
BaeGI GKGCMC 1 cut(s) 1711
BanI GGYRCC 3 cut(s) 1776, 1917, 3825
BbsI GAAGAC 7 cut(s) 7, 613, 2358, 2666, 2945, 3551, 3871
Bbv12I GWGCWC 2 cut(s) 2238, 3777
BbvI GCAGC 6 cut(s) 137, 138, 843, 1081, 1465, 2868
BccI CCATC 6 cut(s) 470, 1309, 1565, 2624, 2743, 3763
BciT130I CCWGG 7 cut(s) 588, 1399, 1560, 2468, 3259, 3674, 3984
BciVI GTATCC 1 cut(s) 3136
BclI TGATCA 1 cut(s) 538
BcoDI GTCTC 6 cut(s) 1024, 1331, 1479, 2063, 2456, 2796
BcuI ACTAGT 2 cut(s) 2834, 3742
BfmI CTRYAG 5 cut(s) 152, 857, 1479, 1887, 3976
BfoI RGCGCY 1 cut(s) 131
BfuAI ACCTGC 1 cut(s) 602
BfuI GTATCC 1 cut(s) 3136
BglI GCCNNNNNGGC 1 cut(s) 93
BisI GCNGC 9 cut(s) 126, 152, 272, 455, 857, 1095, 1479, 1916, 2882
BlsI GCNGC 9 cut(s) 127, 153, 273, 456, 858, 1096, 1480, 1917, 2883
BmcAI AGTACT 3 cut(s) 1700, 1999, 3200
Bme1390I CCNGG 7 cut(s) 588, 1399, 1560, 2468, 3259, 3674, 3984
Bme18I GGWCC 3 cut(s) 1303, 1416, 2045
BmeT110I CYCGRG 2 cut(s) 2508, 3194
BmgT120I GGNCC 5 cut(s) 479, 1303, 1416, 2045, 3526
BmiI GGNNCC 4 cut(s) 1778, 1919, 3812, 3827
BmrFI CCNGG 7 cut(s) 588, 1399, 1560, 2468, 3259, 3674, 3984
BmrI ACTGGG 1 cut(s) 1193
BmsI GCATC 7 cut(s) 1777, 2101, 2558, 2624, 3194, 3908, 4026
BmtI GCTAGC 2 cut(s) 3257, 3899
BmuI ACTGGG 1 cut(s) 1193
BoxI GACNNNNGTC 1 cut(s) 2362
BpiI GAAGAC 7 cut(s) 7, 613, 2358, 2666, 2945, 3551, 3871
BpmI CTGGAG 3 cut(s) 2489, 2600, 3466
Bpu14I TTCGAA 1 cut(s) 3646
BpuEI CTTGAG 7 cut(s) 95, 1723, 2065, 2441, 3223, 3659, 3666
BsaHI GRCGYC 1 cut(s) 96
BsaI GGTCTC 2 cut(s) 1331, 2456
BsaJI CCNNGG 7 cut(s) 19, 1089, 1105, 2062, 2466, 2507, 3258
BsaXI ACNNNNNCTCC 8 cut(s) 1162, 1192, 1283, 1313, 1379, 1409, 3989, 4019
Bsc4I CCNNNNNNNGG 8 cut(s) 488, 593, 660, 2354, 2578, 3461, 3914, 3989
Bse1I ACTGG 7 cut(s) 1199, 1362, 1419, 2284, 2583, 2661, 4004
Bse21I CCTNAGG 1 cut(s) 3909
Bse3DI GCAATG 2 cut(s) 642, 2153
BseBI CCWGG 7 cut(s) 588, 1399, 1560, 2468, 3259, 3674, 3984
BseDI CCNNGG 7 cut(s) 19, 1089, 1105, 2062, 2466, 2507, 3258
BseLI CCNNNNNNNGG 8 cut(s) 488, 593, 660, 2354, 2578, 3461, 3914, 3989
BseMI GCAATG 2 cut(s) 642, 2153
BseMII CTCAG 6 cut(s) 764, 1728, 1985, 2816, 3812, 3900
BseNI ACTGG 7 cut(s) 1199, 1362, 1419, 2284, 2583, 2661, 4004
BseRI GAGGAG 6 cut(s) 48, 761, 2858, 3194, 3474, 3926
BseSI GKGCMC 1 cut(s) 1711
BseXI GCAGC 6 cut(s) 137, 138, 843, 1081, 1465, 2868
BseYI CCCAGC 2 cut(s) 984, 2008
BsgI GTGCAG 3 cut(s) 144, 714, 3058
Bsh1236I CGCG 2 cut(s) 161, 912
BshFI GGCC 5 cut(s) 454, 481, 3249, 3527, 3667
BshNI GGYRCC 3 cut(s) 1776, 1917, 3825
BsiHKAI GWGCWC 2 cut(s) 2238, 3777
BsiHKCI CYCGRG 2 cut(s) 2508, 3194
BslFI GGGAC 5 cut(s) 636, 2080, 3276, 3443, 3930
BslI CCNNNNNNNGG 8 cut(s) 488, 593, 660, 2354, 2578, 3461, 3914, 3989
BsmAI GTCTC 6 cut(s) 1024, 1331, 1479, 2063, 2456, 2796
BsmFI GGGAC 5 cut(s) 636, 2080, 3276, 3443, 3930
BsmI GAATGC 3 cut(s) 26, 1007, 1191
BsnI GGCC 5 cut(s) 454, 481, 3249, 3527, 3667
Bso31I GGTCTC 2 cut(s) 1331, 2456
BsoBI CYCGRG 2 cut(s) 2508, 3194
Bsp119I TTCGAA 1 cut(s) 3646
Bsp1286I GDGCHC 3 cut(s) 1711, 2238, 3777
Bsp1407I TGTACA 1 cut(s) 1833
Bsp68I TCGCGA 1 cut(s) 912
BspANI GGCC 5 cut(s) 454, 481, 3249, 3527, 3667
BspCNI CTCAG 6 cut(s) 765, 1729, 1986, 2817, 3813, 3901
BspFNI CGCG 2 cut(s) 161, 912
BspLI GGNNCC 4 cut(s) 1778, 1919, 3812, 3827
BspMAI CTGCAG 5 cut(s) 156, 861, 1483, 1891, 3980
BspMI ACCTGC 1 cut(s) 602
BspOI GCTAGC 2 cut(s) 3257, 3899
BspPI GGATC 1 cut(s) 933
BspT104I TTCGAA 1 cut(s) 3646
BspT107I GGYRCC 3 cut(s) 1776, 1917, 3825
BspTNI GGTCTC 2 cut(s) 1331, 2456
BsrBI CCGCTC 2 cut(s) 146, 3034
BsrDI GCAATG 2 cut(s) 642, 2153
BsrGI TGTACA 1 cut(s) 1833
BsrI ACTGG 7 cut(s) 1199, 1362, 1419, 2284, 2583, 2661, 4004
BssECI CCNNGG 7 cut(s) 19, 1089, 1105, 2062, 2466, 2507, 3258
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 3 cut(s) 1089, 1105, 2062
Bst2UI CCWGG 7 cut(s) 588, 1399, 1560, 2468, 3259, 3674, 3984
Bst4CI ACNGT 9 cut(s) 645, 1160, 1187, 1473, 2242, 2361, 3434, 3557, 3659
Bst6I CTCTTC 2 cut(s) 1234, 3876
BstACI GRCGYC 1 cut(s) 96
BstAPI GCANNNNNTGC 1 cut(s) 1886
BstAUI TGTACA 1 cut(s) 1833
BstBI TTCGAA 1 cut(s) 3646
BstENI CCTNNNNNAGG 1 cut(s) 3987
BstFNI CGCG 2 cut(s) 161, 912
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 2 cut(s) 130, 276
BstMAI GTCTC 6 cut(s) 1024, 1331, 1479, 2063, 2456, 2796
BstNI CCWGG 7 cut(s) 588, 1399, 1560, 2468, 3259, 3674, 3984
BstNSI RCATGY 7 cut(s) 299, 834, 970, 1007, 1492, 2312, 3544
BstPAI GACNNNNGTC 1 cut(s) 2362
BstSCI CCNGG 7 cut(s) 586, 1397, 1558, 2466, 3257, 3672, 3982
BstSFI CTRYAG 5 cut(s) 152, 857, 1479, 1887, 3976
BstSLI GKGCMC 1 cut(s) 1711
BstUI CGCG 2 cut(s) 161, 912
BstV1I GCAGC 6 cut(s) 137, 138, 843, 1081, 1465, 2868
BstV2I GAAGAC 7 cut(s) 7, 613, 2358, 2666, 2945, 3551, 3871
BstX2I RGATCY 1 cut(s) 938
BstYI RGATCY 1 cut(s) 938
Bsu36I CCTNAGG 1 cut(s) 3909
BsuI GTATCC 1 cut(s) 3136
BsuRI GGCC 5 cut(s) 454, 481, 3249, 3527, 3667
BtgZI GCGATG 1 cut(s) 2762
BtsI GCAGTG 2 cut(s) 1880, 3959
BtsIMutI CAGTG 6 cut(s) 1192, 1880, 1991, 2238, 3553, 3959
BtuMI TCGCGA 1 cut(s) 912
BveI ACCTGC 1 cut(s) 602
CaiI CAGNNNCTG 1 cut(s) 40
CfoI GCGC 2 cut(s) 130, 276
Cfr13I GGNCC 5 cut(s) 479, 1303, 1416, 2045, 3526
CseI GACGC 3 cut(s) 85, 167, 2314
Csp6I GTAC 8 cut(s) 317, 1699, 1777, 1834, 1937, 1998, 2711, 3199
CspCI CAANNNNNGTGG 2 cut(s) 281, 316
CviQI GTAC 8 cut(s) 317, 1699, 1777, 1834, 1937, 1998, 2711, 3199
DraIII CACNNNGTG 1 cut(s) 737
Eam1104I CTCTTC 2 cut(s) 1234, 3876
EarI CTCTTC 2 cut(s) 1234, 3876
EciI GGCGGA 1 cut(s) 891
Eco130I CCWWGG 3 cut(s) 1089, 1105, 2062
Eco31I GGTCTC 2 cut(s) 1331, 2456
Eco32I GATATC 2 cut(s) 387, 3721
Eco47I GGWCC 3 cut(s) 1303, 1416, 2045
Eco47III AGCGCT 1 cut(s) 129
Eco57I CTGAAG 5 cut(s) 17, 1784, 2957, 3545, 3865
Eco81I CCTNAGG 1 cut(s) 3909
Eco88I CYCGRG 2 cut(s) 2508, 3194
EcoNI CCTNNNNNAGG 1 cut(s) 3987
EcoO109I RGGNCCY 2 cut(s) 2045, 3526
EcoRI GAATTC 1 cut(s) 346
EcoRII CCWGG 7 cut(s) 586, 1397, 1558, 2466, 3257, 3672, 3982
EcoRV GATATC 2 cut(s) 387, 3721
EcoT14I CCWWGG 3 cut(s) 1089, 1105, 2062
EcoT22I ATGCAT 3 cut(s) 1009, 1449, 3510
ErhI CCWWGG 3 cut(s) 1089, 1105, 2062
FalI AAGNNNNNCTT 2 cut(s) 2789, 2821
FaqI GGGAC 5 cut(s) 636, 2080, 3276, 3443, 3930
FauI CCCGC 1 cut(s) 2630
FauNDI CATATG 1 cut(s) 4028
FbaI TGATCA 1 cut(s) 538
FblI GTMKAC 2 cut(s) 99, 2399
Fnu4HI GCNGC 9 cut(s) 126, 152, 272, 455, 857, 1095, 1479, 1916, 2882
Fsp4HI GCNGC 9 cut(s) 126, 152, 272, 455, 857, 1095, 1479, 1916, 2882
GlaI GCGC 2 cut(s) 129, 275
GluI GCNGC 9 cut(s) 126, 152, 272, 455, 857, 1095, 1479, 1916, 2882
GsaI CCCAGC 2 cut(s) 988, 2012
GsuI CTGGAG 3 cut(s) 2489, 2600, 3466
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 5 cut(s) 454, 481, 3249, 3527, 3667
HgaI GACGC 3 cut(s) 85, 167, 2314
HhaI GCGC 2 cut(s) 130, 276
Hin1I GRCGYC 1 cut(s) 96
Hin6I GCGC 2 cut(s) 128, 274
HinP1I GCGC 2 cut(s) 128, 274
HincII GTYRAC 3 cut(s) 54, 100, 3328
HindII GTYRAC 3 cut(s) 54, 100, 3328
HindIII AAGCTT 3 cut(s) 1039, 2618, 2813
HpaI GTTAAC 2 cut(s) 54, 3328
HphI GGTGA 5 cut(s) 1472, 3149, 3284, 3394, 4052
Hpy166II GTNNAC 6 cut(s) 54, 100, 1352, 2400, 3328, 3959
Hpy8I GTNNAC 6 cut(s) 54, 100, 1352, 2400, 3328, 3959
Hpy99I CGWCG 1 cut(s) 101
HpyCH4III ACNGT 9 cut(s) 645, 1160, 1187, 1473, 2242, 2361, 3434, 3557, 3659
HpyCH4IV ACGT 4 cut(s) 279, 2887, 3072, 3324
HpySE526I ACGT 4 cut(s) 279, 2887, 3072, 3324
Hsp92I GRCGYC 1 cut(s) 96
HspAI GCGC 2 cut(s) 128, 274
KpnI GGTACC 1 cut(s) 1780
Ksp22I TGATCA 1 cut(s) 538
KspAI GTTAAC 2 cut(s) 54, 3328
Lsp1109I GCAGC 6 cut(s) 137, 138, 843, 1081, 1465, 2868
LweI GCATC 7 cut(s) 1777, 2101, 2558, 2624, 3194, 3908, 4026
MaeII ACGT 4 cut(s) 279, 2887, 3072, 3324
MbiI CCGCTC 2 cut(s) 146, 3034
MfeI CAATTG 4 cut(s) 606, 2403, 2642, 2655
MflI RGATCY 1 cut(s) 938
MhlI GDGCHC 3 cut(s) 1711, 2238, 3777
MlyI GAGTC 6 cut(s) 778, 1110, 1180, 2772, 3690, 3912
MmeI TCCRAC 2 cut(s) 2298, 3696
Mph1103I ATGCAT 3 cut(s) 1009, 1449, 3510
MroXI GAANNNNTTC 2 cut(s) 3733, 3870
MslI CAYNNNNRTG 5 cut(s) 1345, 2718, 2857, 2947, 2961
MspA1I CMGCKG 1 cut(s) 3885
MspR9I CCNGG 7 cut(s) 588, 1399, 1560, 2468, 3259, 3674, 3984
MunI CAATTG 4 cut(s) 606, 2403, 2642, 2655
Mva1269I GAATGC 3 cut(s) 26, 1007, 1191
MvaI CCWGG 7 cut(s) 588, 1399, 1560, 2468, 3259, 3674, 3984
MvnI CGCG 2 cut(s) 161, 912
NdeI CATATG 1 cut(s) 4028
NheI GCTAGC 2 cut(s) 3253, 3895
NlaIV GGNNCC 4 cut(s) 1778, 1919, 3812, 3827
NmeAIII GCCGAG 1 cut(s) 483
NmuCI GTSAC 9 cut(s) 697, 833, 901, 1338, 1467, 2095, 2940, 3290, 3395
NruI TCGCGA 1 cut(s) 912
NsiI ATGCAT 3 cut(s) 1009, 1449, 3510
NspI RCATGY 7 cut(s) 299, 834, 970, 1007, 1492, 2312, 3544
NspV TTCGAA 1 cut(s) 3646
PaeR7I CTCGAG 1 cut(s) 3194
PciI ACATGT 2 cut(s) 830, 1488
PctI GAATGC 3 cut(s) 26, 1007, 1191
PdmI GAANNNNTTC 2 cut(s) 3733, 3870
PflMI CCANNNNNTGG 2 cut(s) 593, 660
PfoI TCCNGGA 1 cut(s) 3672
PkrI GCNGC 9 cut(s) 127, 153, 273, 456, 858, 1096, 1480, 1917, 2883
PleI GAGTC 6 cut(s) 777, 1109, 1179, 2772, 3690, 3912
PpsI GAGTC 6 cut(s) 777, 1109, 1179, 2772, 3690, 3912
PpuMI RGGWCCY 1 cut(s) 2045
PscI ACATGT 2 cut(s) 830, 1488
PshAI GACNNNNGTC 1 cut(s) 2362
PsiI TTATAA 1 cut(s) 2667
Psp5II RGGWCCY 1 cut(s) 2045
Psp6I CCWGG 7 cut(s) 586, 1397, 1558, 2466, 3257, 3672, 3982
PspFI CCCAGC 2 cut(s) 984, 2008
PspGI CCWGG 7 cut(s) 586, 1397, 1558, 2466, 3257, 3672, 3982
PspN4I GGNNCC 4 cut(s) 1778, 1919, 3812, 3827
PspPI GGNCC 5 cut(s) 479, 1303, 1416, 2045, 3526
PspPPI RGGWCCY 1 cut(s) 2045
PspXI VCTCGAGB 1 cut(s) 3194
PstI CTGCAG 5 cut(s) 156, 861, 1483, 1891, 3980
PstNI CAGNNNCTG 1 cut(s) 40
PsuI RGATCY 1 cut(s) 938
PvuII CAGCTG 1 cut(s) 3885
RruI TCGCGA 1 cut(s) 912
RsaI GTAC 8 cut(s) 318, 1700, 1778, 1835, 1938, 1999, 2712, 3200
RsaNI GTAC 8 cut(s) 317, 1699, 1777, 1834, 1937, 1998, 2711, 3199
RseI CAYNNNNRTG 5 cut(s) 1345, 2718, 2857, 2947, 2961
SalI GTCGAC 1 cut(s) 98
SatI GCNGC 9 cut(s) 126, 152, 272, 455, 857, 1095, 1479, 1916, 2882
Sau96I GGNCC 5 cut(s) 479, 1303, 1416, 2045, 3526
ScaI AGTACT 3 cut(s) 1700, 1999, 3200
SchI GAGTC 6 cut(s) 778, 1110, 1180, 2772, 3690, 3912
ScrFI CCNGG 7 cut(s) 588, 1399, 1560, 2468, 3259, 3674, 3984
SduI GDGCHC 3 cut(s) 1711, 2238, 3777
SfaNI GCATC 7 cut(s) 1777, 2101, 2558, 2624, 3194, 3908, 4026
SfcI CTRYAG 5 cut(s) 152, 857, 1479, 1887, 3976
Sfr274I CTCGAG 1 cut(s) 3194
SfuI TTCGAA 1 cut(s) 3646
SinI GGWCC 3 cut(s) 1303, 1416, 2045
SlaI CTCGAG 1 cut(s) 3194
SmiMI CAYNNNNRTG 5 cut(s) 1345, 2718, 2857, 2947, 2961
SmlI CTYRAG 8 cut(s) 110, 1702, 2080, 2420, 3194, 3202, 3638, 3681
SmoI CTYRAG 8 cut(s) 110, 1702, 2080, 2420, 3194, 3202, 3638, 3681
SpeI ACTAGT 2 cut(s) 2834, 3742
SspI AATATT 1 cut(s) 1152
StyD4I CCNGG 7 cut(s) 586, 1397, 1558, 2466, 3257, 3672, 3982
StyI CCWWGG 3 cut(s) 1089, 1105, 2062
TaaI ACNGT 9 cut(s) 645, 1160, 1187, 1473, 2242, 2361, 3434, 3557, 3659
TaiI ACGT 4 cut(s) 282, 2890, 3075, 3327
TaqI TCGA 8 cut(s) 45, 99, 1645, 2338, 2615, 3195, 3498, 3646
TatI WGTACW 4 cut(s) 1698, 1833, 1997, 3198
TauI GCSGC 3 cut(s) 274, 457, 1918
TscAI CASTG 6 cut(s) 1192, 1887, 1998, 2245, 3560, 3966
TseFI GTSAC 9 cut(s) 697, 833, 901, 1338, 1467, 2095, 2940, 3290, 3395
TseI GCWGC 6 cut(s) 125, 151, 856, 1094, 1478, 2881
Tsp45I GTSAC 9 cut(s) 697, 833, 901, 1338, 1467, 2095, 2940, 3290, 3395
TspGWI ACGGA 1 cut(s) 2805
TspRI CASTG 6 cut(s) 1192, 1887, 1998, 2245, 3560, 3966
Van91I CCANNNNNTGG 2 cut(s) 593, 660
VpaK11BI GGWCC 3 cut(s) 1303, 1416, 2045
XagI CCTNNNNNAGG 1 cut(s) 3987
XapI RAATTY 8 cut(s) 346, 759, 1234, 1283, 1583, 1592, 2688, 3091
XbaI TCTAGA 1 cut(s) 3604
XceI RCATGY 7 cut(s) 299, 834, 970, 1007, 1492, 2312, 3544
XhoI CTCGAG 1 cut(s) 3194
XmiI GTMKAC 2 cut(s) 99, 2399
XmnI GAANNNNTTC 2 cut(s) 3733, 3870
ZrmI AGTACT 3 cut(s) 1700, 1999, 3200
Zsp2I ATGCAT 3 cut(s) 1009, 1449, 3510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.