MD11G1043100.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000070
Physical Location & Seq
Reverse (-)
3680668 .. 3683593
2926 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1043100.v1.1.491

Sequence Viewer

Length: 2886 bp
ATGTCATTGGGAGAGGTCTTTATTTCTGGGTTACTTCAAGTGTTACTTGAAAGGTTGACATCTTGTGAGACGCTGAGCTACTTTAGACGCCTACTTGGCGTTGGCAAAGAGCTGAACAAATGGAAGTCAATGTTGTCTACTATTCGAGAAGTGCTGAATGACGCGGAGGACAAGCAATTGACGGACCAAGCAGTGAAATTGTGGCTGGATGATCTCACAGACTTGGCCTATGATGTGGAAGACATATTGGACAAGTTTTCAACAGAGATGTTGCGACGCCAAATGAAGGATCAACATGGGGCTACCACAAACAAGGCATGGGGTTTGTTTTCAGATGTTAAATTTAGCTGTAACCTGAGCTCAAAAATAAAGAAGATTACTGATCGGCAACATGAGATATTGGAAGTAAAAAAACAGCTTGGTTTAAATGAGGGGACACCATCTACTAAAGCATGGCACGTGCCACCGAGTTCCCACCTACCAGAAGGACCTGTGATTGGAAGGGATACAGACAAGACGAAGATTATTGACTTCTTGTTAAAAGAAGCACCCAATGCCGTCAACTTTCATGTAGTTGCCATTCTCGGTATGCCTGGAGTGGGAAAGACTACACTTGCAAGACATGTTTATGATGATGACGCGACAAAAGAGTTTAACCGAAAAGTATGGGTATCTGTGTCGGATGACTTCAATCTTGAAAGAGTGACAAAGGCAATTCTTGAATCCGCCACATCTGGTCTTGCAAAGGAGTACAAGGAATTCAACCAGGTTCAAGAAAGTTTGAGTAAGGAGCTAGCAGGAAAAAAGTTTCTAATTGTTTTAGACGATGTTTGGAATACATGTGTCTATGATTTGTGGATAAAATTACAGTCCCCCTTCCGTGTTGGAGCATTAGGAAGTAAGATAATTGTGACAACACGTGATGCAAATGTTGCAAACATGATGGGATCCACTGTTTATCAATTGGATTGCATGACAAATGATCAATGTTGGGAAGTCTTTAAGCAACATTCCCGATTGGACATTACCAAAAGGCCGCAGAAGTTTGAGTTCATAAAGGAGACAATTGTTGCAAAATGTTGCGGATTGCCATTGGCTGCAAGGACTCTTGGTGGTCTTCTACGCTGTAAACAAGTAGAAGAATGGGAGGAAATCTTAAACAACAAGATGTGGAGTCTATCAGACAATAGTGGCATTCTCCCAGTATTAAAATTGAGCTATCACTATCTCCCTTCGAGTTTGAAACGGTGTTTCGCCTATTGTTCCCTACTTCCAAATGACTATGAGTTCGGGGAGAAGCAGTTGATCCTTCTGTGGATGGCAGAAGGTTTGATTCAACAAAGAGTAGAGGACAAGAAACAACTGGAAGAAGTAGGCTGTGACTATTTTCAAGAGCTATTGTCCAGGTCATTATTTCAAAAGGGAAGCAAAAGTAATGATAAGTATGTAATGCATGACCTTGTTGGTGATTTGGCGTGGTGGGCTGCAGGTGAAATATGTTTTTTATTGGAGGACAAAGGAAATGGGGACTTGCATCCAGCGGCTCGTCATATGTCTTATGTCAGTGGTACGTATGATGGGGTTAAGAAATTTGAGGCCATATCCAGAGTTAAACATTTGAGGACATTCATGCCATTTGCACAAGATTTATCTCGGTTTCCAGTAATTGAACCGAACTATCTAACTCGTAGGGTTACGTTTGATCTATTGCCAGAACTACGAATCTTACGAGTGCTTTCTTTGGATGGCTATGGAATAACTGAGCTGCCCAATTCAATTGGTAAATTGTGGAATCTACGGTATCTGGACCTTTCTTATACTGAGATAAAGAGTTTGCCTCCATCAACAACCACTCTTTGCAATCTGCAAACATTTTTATTAGAAGGTTGTTCTAAATTGAAGGCATTGCCCGCAAACATGAGTAATTTAATTAGTTTGCGGCATCTCAACAGTTCCATTCGATTGAAAGGAATGCCTCCACATCTAGGTCGATTGACGAATCTGCAATCCTTGTCATATTTTGTGGTAGGCAAAAGTAGTGATCAGTCTAAGATAAGAGAGATAGGGTCCCTATTGCATCTCCGAGGGACATTGTGGCTCTCAAGATTGGAAAATGTGGTTGATGCTGAGGATGCTAGTGGGGCCAAGTTAAGACACAAGGAGAAGCTTGATTCATTGAAGCTGGAATGGTCTGATTTGAGGGGATGGAGGGAAATGGAGTTGGGCGTGCTTGACATGTTACAACCTCCTAAAAATCTTAAAGAGCTCACCATTTTTGGTTATGGTGGATCTAATTTTTCATCATGGATTGGAGATCCTCTGTTCACTAACATGGTCCGTGTATGCTTATCCTATTGTGAAAATTGTCATATATTGCCACCGGTTGGACAGTTGCGTTGCCTTAAAGAACTTCATATCATGGGAATGACTGCTGTGAAAATCGTTGGTCCCGAATTCTATGGAAAGGGTAGCTTGCCTTTTCGAGTATTAGAGATTCTCAAGTTTTCGGAGATGGAGCACTGGGAGAAATGGCTGCCTTTGGATCGGGATAAGGGAAGTGGTGTTTTCCCTAGCCTAAAAGTGTTTTCAATCAGATACTGCCCTAAACTGGAGGGCGAGTTGCCGAAGAAACTTAATTCGTTAGCAAAGCTTCAGATTGTTGGATGCAAGGAATTGGTGGTGGAAATTGCCAATTATGAACACGCAAGTGAACTAAGCATCCATGGCTGCAAAGCGCTAGTGCATACTAGTGCAGAGGTCGACTTTAAGTTATTACAGACCATGTGGCTTTCAAATATTTTGGAGCCGCGGTTGGGAGCAAGGGGACTTATGAAAGGATTGGGCAAGATTAAGGAGTTGAAGATTACTGATGTTTCCCACACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

962

Amino Acids

109.08

Weight (kDa)

8.27

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 99 6.2e-24 Rx N-terminal domain
NB-ARC PF00931 169 - 336 2.8e-35 NB-ARC domain
WHD_DRP PF23559 424 - 492 5.6e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 570 - 866 3e-25 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 685 - 809 4e-34 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1478
Acc36I ACCTGC 1 cut(s) 1478
AccB7I CCANNNNNTGG 1 cut(s) 2384
AccI GTMKAC 2 cut(s) 137, 2760
AccII CGCG 3 cut(s) 164, 641, 2809
AciI CCGC 9 cut(s) 164, 726, 1037, 1083, 1541, 1911, 1939, 2807, 2809
AclWI GGATC 7 cut(s) 297, 942, 955, 1300, 2296, 2309, 2550
AcsI RAATTY 4 cut(s) 341, 758, 1589, 2453
AcuI CTGAAG 1 cut(s) 2636
AcvI CACGTG 2 cut(s) 460, 920
AcyI GRCGYC 2 cut(s) 88, 277
AfaI GTAC 2 cut(s) 752, 1570
AfeI AGCGCT 1 cut(s) 2736
AfiI CCNNNNNNNGG 7 cut(s) 286, 497, 599, 1985, 2384, 2608, 2813
AflIII ACRYGT 4 cut(s) 622, 839, 917, 2235
AgeI ACCGGT 1 cut(s) 2380
AhlI ACTAGT 1 cut(s) 2747
AjnI CCWGG 3 cut(s) 592, 765, 1403
AleI CACNNNNGTG 1 cut(s) 2706
AloI GAACNNNNNNTCC 2 cut(s) 2703, 2735
Alw21I GWGCWC 3 cut(s) 362, 2268, 2520
Alw26I GTCTC 2 cut(s) 62, 1055
AlwI GGATC 7 cut(s) 297, 942, 955, 1300, 2296, 2309, 2550
AlwNI CAGNNNCTG 1 cut(s) 2598
Aor51HI AGCGCT 1 cut(s) 2736
AoxI GGCC 4 cut(s) 225, 1034, 1596, 2142
ApeKI GCWGC 5 cut(s) 1097, 1484, 1765, 2533, 2727
ApoI RAATTY 4 cut(s) 341, 758, 1589, 2453
ArsI GACNNNNNNTTYG 2 cut(s) 1271, 1303
AsiGI ACCGGT 1 cut(s) 2380
AspLEI GCGC 1 cut(s) 2737
AspS9I GGNCC 7 cut(s) 184, 488, 1807, 2067, 2142, 2335, 2447
AsuHPI GGTGA 3 cut(s) 1478, 1502, 2260
AsuNHI GCTAGC 1 cut(s) 793
AvaII GGWCC 6 cut(s) 184, 488, 1807, 2067, 2335, 2447
BamHI GGATCC 1 cut(s) 947
BanII GRGCYC 2 cut(s) 362, 2268
BbrPI CACGTG 2 cut(s) 460, 920
BbsI GAAGAC 2 cut(s) 246, 1109
Bbv12I GWGCWC 3 cut(s) 362, 2268, 2520
BbvCI CCTCAGC 1 cut(s) 2127
BbvI GCAGC 5 cut(s) 1084, 1471, 1752, 2520, 2714
BccI CCATC 8 cut(s) 448, 937, 1312, 1571, 1739, 1849, 2199, 2506
BceAI ACGGC 1 cut(s) 542
BciT130I CCWGG 3 cut(s) 594, 767, 1405
BciVI GTATCC 1 cut(s) 499
BclI TGATCA 2 cut(s) 982, 2041
BcoDI GTCTC 2 cut(s) 62, 1055
BcuI ACTAGT 1 cut(s) 2747
BfaI CTAG 6 cut(s) 794, 1985, 2136, 2571, 2738, 2748
BfmI CTRYAG 1 cut(s) 1485
BfoI RGCGCY 1 cut(s) 2738
BfuAI ACCTGC 1 cut(s) 1478
BfuI GTATCC 1 cut(s) 499
BglI GCCNNNNNGGC 1 cut(s) 96
BisI GCNGC 9 cut(s) 1037, 1098, 1485, 1542, 1766, 1940, 2534, 2728, 2807
BlpI GCTNAGC 1 cut(s) 74
BlsI GCNGC 9 cut(s) 1038, 1099, 1486, 1543, 1767, 1941, 2535, 2729, 2808
Bme1390I CCNGG 3 cut(s) 594, 767, 1405
Bme18I GGWCC 6 cut(s) 184, 488, 1807, 2067, 2335, 2447
BmgT120I GGNCC 7 cut(s) 184, 488, 1807, 2067, 2142, 2335, 2447
BmiI GGNNCC 6 cut(s) 949, 2068, 2069, 2143, 2449, 2805
BmrFI CCNGG 3 cut(s) 594, 767, 1405
BmrI ACTGGG 2 cut(s) 1196, 2530
BmsI GCATC 8 cut(s) 913, 1543, 1951, 2086, 2113, 2122, 2654, 2727
BmtI GCTAGC 1 cut(s) 797
BmuI ACTGGG 2 cut(s) 1196, 2530
BpiI GAAGAC 2 cut(s) 246, 1109
BplI GAGNNNNNCTC 2 cut(s) 1822, 1854
BpmI CTGGAG 2 cut(s) 615, 2630
Bpu10I CCTNAGC 2 cut(s) 356, 2127
Bpu1102I GCTNAGC 1 cut(s) 74
BpuEI CTTGAG 2 cut(s) 2086, 2483
BsaAI YACGTR 3 cut(s) 460, 920, 1572
BsaHI GRCGYC 2 cut(s) 88, 277
BsaJI CCNNGG 3 cut(s) 2083, 2722, 2807
BsaWI WCCGGW 1 cut(s) 2380
BsaXI ACNNNNNCTCC 6 cut(s) 1286, 1316, 2603, 2633, 2795, 2825
Bsc4I CCNNNNNNNGG 7 cut(s) 286, 497, 599, 1985, 2384, 2608, 2813
Bse118I RCCGGY 1 cut(s) 2380
Bse1I ACTGG 5 cut(s) 1202, 1368, 1661, 2525, 2613
Bse3DI GCAATG 1 cut(s) 1904
BseBI CCWGG 3 cut(s) 594, 767, 1405
BseDI CCNNGG 3 cut(s) 2083, 2722, 2807
BseGI GGATG 9 cut(s) 214, 688, 1323, 1534, 1750, 2137, 2210, 2669, 2718
BseLI CCNNNNNNNGG 7 cut(s) 286, 497, 599, 1985, 2384, 2608, 2813
BseMI GCAATG 1 cut(s) 1904
BseMII CTCAG 5 cut(s) 65, 347, 1752, 1812, 2118
BseNI ACTGG 5 cut(s) 1202, 1368, 1661, 2525, 2613
BseXI GCAGC 5 cut(s) 1084, 1471, 1752, 2520, 2714
BsgI GTGCAG 1 cut(s) 2772
Bsh1236I CGCG 3 cut(s) 164, 641, 2809
BshFI GGCC 4 cut(s) 227, 1036, 1598, 2144
BshTI ACCGGT 1 cut(s) 2380
BsiHKAI GWGCWC 3 cut(s) 362, 2268, 2520
BsiSI CCGG 1 cut(s) 2381
BslFI GGGAC 7 cut(s) 448, 856, 1541, 2053, 2101, 2433, 2838
BslI CCNNNNNNNGG 7 cut(s) 286, 497, 599, 1985, 2384, 2608, 2813
BsmAI GTCTC 2 cut(s) 62, 1055
BsmBI CGTCTC 1 cut(s) 62
BsmFI GGGAC 7 cut(s) 448, 856, 1541, 2053, 2101, 2433, 2838
BsmI GAATGC 2 cut(s) 1194, 1977
BsnI GGCC 4 cut(s) 227, 1036, 1598, 2144
Bsp1286I GDGCHC 3 cut(s) 362, 2268, 2520
Bsp1720I GCTNAGC 1 cut(s) 74
Bsp19I CCATGG 1 cut(s) 2722
BspACI CCGC 9 cut(s) 164, 726, 1037, 1083, 1541, 1911, 1939, 2807, 2809
BspANI GGCC 4 cut(s) 227, 1036, 1598, 2144
BspCNI CTCAG 5 cut(s) 66, 348, 1753, 1813, 2119
BspFNI CGCG 3 cut(s) 164, 641, 2809
BspLI GGNNCC 6 cut(s) 949, 2068, 2069, 2143, 2449, 2805
BspMAI CTGCAG 1 cut(s) 1489
BspMI ACCTGC 1 cut(s) 1478
BspOI GCTAGC 1 cut(s) 797
BspPI GGATC 7 cut(s) 297, 942, 955, 1300, 2296, 2309, 2550
BsrDI GCAATG 1 cut(s) 1904
BsrFI RCCGGY 1 cut(s) 2380
BsrI ACTGG 5 cut(s) 1202, 1368, 1661, 2525, 2613
BssAI RCCGGY 1 cut(s) 2380
BssECI CCNNGG 3 cut(s) 2083, 2722, 2807
BssNI GRCGYC 2 cut(s) 88, 277
BssT1I CCWWGG 1 cut(s) 2722
Bst2UI CCWGG 3 cut(s) 594, 767, 1405
Bst4CI ACNGT 6 cut(s) 870, 955, 1248, 1800, 1952, 2391
BstACI GRCGYC 2 cut(s) 88, 277
BstAPI GCANNNNNTGC 2 cut(s) 554, 932
BstBAI YACGTR 3 cut(s) 460, 920, 1572
BstC8I GCNNGC 4 cut(s) 795, 1911, 2228, 2474
BstDEI CTNAG 7 cut(s) 74, 356, 1761, 1821, 2049, 2127, 2714
BstDSI CCRYGG 2 cut(s) 2722, 2807
BstF5I GGATG 9 cut(s) 214, 688, 1323, 1534, 1750, 2137, 2210, 2669, 2718
BstFNI CGCG 3 cut(s) 164, 641, 2809
BstH2I RGCGCY 1 cut(s) 2738
BstHHI GCGC 1 cut(s) 2737
BstMAI GTCTC 2 cut(s) 62, 1055
BstMWI GCNNNNNNNGC 8 cut(s) 96, 554, 932, 1481, 1910, 2132, 2141, 2724
BstNI CCWGG 3 cut(s) 594, 767, 1405
BstNSI RCATGY 3 cut(s) 626, 843, 2239
BstSCI CCNGG 3 cut(s) 592, 765, 1403
BstSFI CTRYAG 1 cut(s) 1485
BstSNI TACGTA 1 cut(s) 1572
BstUI CGCG 3 cut(s) 164, 641, 2809
BstV1I GCAGC 5 cut(s) 1084, 1471, 1752, 2520, 2714
BstV2I GAAGAC 2 cut(s) 246, 1109
BstX2I RGATCY 3 cut(s) 947, 2288, 2314
BstYI RGATCY 3 cut(s) 947, 2288, 2314
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 4 cut(s) 227, 1036, 1598, 2144
BtgI CCRYGG 2 cut(s) 2722, 2807
BtsCI GGATG 9 cut(s) 214, 688, 1323, 1534, 1750, 2137, 2210, 2669, 2718
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 4 cut(s) 198, 951, 1570, 2518
BveI ACCTGC 1 cut(s) 1478
Cac8I GCNNGC 4 cut(s) 795, 1911, 2228, 2474
CaiI CAGNNNCTG 1 cut(s) 2598
CfoI GCGC 1 cut(s) 2737
Cfr10I RCCGGY 1 cut(s) 2380
Cfr13I GGNCC 7 cut(s) 184, 488, 1807, 2067, 2142, 2335, 2447
Cfr42I CCGCGG 1 cut(s) 2810
CseI GACGC 5 cut(s) 79, 96, 170, 285, 647
CsiI ACCWGGT 1 cut(s) 765
Csp6I GTAC 2 cut(s) 751, 1569
CspAI ACCGGT 1 cut(s) 2380
CviQI GTAC 2 cut(s) 751, 1569
DdeI CTNAG 7 cut(s) 74, 356, 1761, 1821, 2049, 2127, 2714
DraI TTTAAA 1 cut(s) 426
EciI GGCGGA 1 cut(s) 715
Ecl136II GAGCTC 2 cut(s) 360, 2266
Eco105I TACGTA 1 cut(s) 1572
Eco130I CCWWGG 1 cut(s) 2722
Eco24I GRGCYC 2 cut(s) 362, 2268
Eco47I GGWCC 6 cut(s) 184, 488, 1807, 2067, 2335, 2447
Eco47III AGCGCT 1 cut(s) 2736
Eco53kI GAGCTC 2 cut(s) 360, 2266
Eco57I CTGAAG 1 cut(s) 2636
Eco72I CACGTG 2 cut(s) 460, 920
EcoICRI GAGCTC 2 cut(s) 360, 2266
EcoO109I RGGNCCY 2 cut(s) 488, 2067
EcoRI GAATTC 2 cut(s) 758, 2453
EcoRII CCWGG 3 cut(s) 592, 765, 1403
EcoT14I CCWWGG 1 cut(s) 2722
EcoT22I ATGCAT 1 cut(s) 1455
EcoT38I GRGCYC 2 cut(s) 362, 2268
ErhI CCWWGG 1 cut(s) 2722
Esp3I CGTCTC 1 cut(s) 62
FalI AAGNNNNNCTT 4 cut(s) 30, 62, 2456, 2488
FaqI GGGAC 7 cut(s) 448, 856, 1541, 2053, 2101, 2433, 2838
FauI CCCGC 1 cut(s) 1918
FauNDI CATATG 1 cut(s) 1551
FbaI TGATCA 2 cut(s) 982, 2041
FblI GTMKAC 2 cut(s) 137, 2760
Fnu4HI GCNGC 9 cut(s) 1037, 1098, 1485, 1542, 1766, 1940, 2534, 2728, 2807
FokI GGATG 9 cut(s) 221, 695, 1330, 1521, 1757, 2144, 2217, 2676, 2705
FriOI GRGCYC 2 cut(s) 362, 2268
Fsp4HI GCNGC 9 cut(s) 1037, 1098, 1485, 1542, 1766, 1940, 2534, 2728, 2807
FspBI CTAG 6 cut(s) 794, 1985, 2136, 2571, 2738, 2748
GlaI GCGC 1 cut(s) 2736
GluI GCNGC 9 cut(s) 1037, 1098, 1485, 1542, 1766, 1940, 2534, 2728, 2807
GsuI CTGGAG 2 cut(s) 615, 2630
HaeII RGCGCY 1 cut(s) 2738
HaeIII GGCC 4 cut(s) 227, 1036, 1598, 2144
HapII CCGG 1 cut(s) 2381
HgaI GACGC 5 cut(s) 79, 96, 170, 285, 647
HhaI GCGC 1 cut(s) 2737
Hin1I GRCGYC 2 cut(s) 88, 277
Hin6I GCGC 1 cut(s) 2735
HinP1I GCGC 1 cut(s) 2735
HincII GTYRAC 3 cut(s) 57, 562, 2761
HindII GTYRAC 3 cut(s) 57, 562, 2761
HindIII AAGCTT 2 cut(s) 2165, 2648
HinfI GANTC 9 cut(s) 722, 1105, 1174, 1333, 1722, 1792, 1999, 2171, 2494
HpaII CCGG 1 cut(s) 2381
HphI GGTGA 3 cut(s) 1478, 1502, 2260
Hpy166II GTNNAC 7 cut(s) 57, 138, 562, 1130, 2325, 2711, 2761
Hpy188I TCNGA 8 cut(s) 334, 682, 1183, 2084, 2194, 2509, 2594, 2655
Hpy8I GTNNAC 7 cut(s) 57, 138, 562, 1130, 2325, 2711, 2761
Hpy99I CGWCG 1 cut(s) 279
HpyAV CCTTC 9 cut(s) 280, 479, 495, 886, 1242, 1319, 1319, 1877, 1894
HpyCH4III ACNGT 6 cut(s) 870, 955, 1248, 1800, 1952, 2391
HpyCH4IV ACGT 4 cut(s) 459, 919, 1571, 1697
HpyF10VI GCNNNNNNNGC 8 cut(s) 96, 554, 932, 1481, 1910, 2132, 2141, 2724
HpyF3I CTNAG 7 cut(s) 74, 356, 1761, 1821, 2049, 2127, 2714
HpySE526I ACGT 4 cut(s) 459, 919, 1571, 1697
Hsp92I GRCGYC 2 cut(s) 88, 277
HspAI GCGC 1 cut(s) 2735
KflI GGGWCCC 1 cut(s) 2067
Ksp22I TGATCA 2 cut(s) 982, 2041
KspI CCGCGG 1 cut(s) 2810
LmnI GCTCC 5 cut(s) 790, 887, 2515, 2803, 2816
Lsp1109I GCAGC 5 cut(s) 1084, 1471, 1752, 2520, 2714
LweI GCATC 8 cut(s) 913, 1543, 1951, 2086, 2113, 2122, 2654, 2727
MabI ACCWGGT 1 cut(s) 765
MaeI CTAG 6 cut(s) 794, 1985, 2136, 2571, 2738, 2748
MaeII ACGT 4 cut(s) 459, 919, 1571, 1697
MaeIII GTNAC 8 cut(s) 30, 42, 350, 703, 910, 1379, 1693, 2238
MboII GAAGA 8 cut(s) 251, 385, 532, 1109, 1151, 1379, 2638, 2872
MfeI CAATTG 4 cut(s) 176, 962, 1065, 1776
MflI RGATCY 3 cut(s) 947, 2288, 2314
MhlI GDGCHC 3 cut(s) 362, 2268, 2520
MlyI GAGTC 2 cut(s) 1099, 1183
MmeI TCCRAC 4 cut(s) 660, 865, 2365, 2641
Mph1103I ATGCAT 1 cut(s) 1455
MslI CAYNNNNRTG 5 cut(s) 627, 2330, 2423, 2706, 2748
MspA1I CMGCKG 2 cut(s) 1541, 2809
MspI CCGG 1 cut(s) 2381
MspR9I CCNGG 3 cut(s) 594, 767, 1405
MunI CAATTG 4 cut(s) 176, 962, 1065, 1776
Mva1269I GAATGC 2 cut(s) 1194, 1977
MvaI CCWGG 3 cut(s) 594, 767, 1405
MvnI CGCG 3 cut(s) 164, 641, 2809
MwoI GCNNNNNNNGC 8 cut(s) 96, 554, 932, 1481, 1910, 2132, 2141, 2724
NcoI CCATGG 1 cut(s) 2722
NdeI CATATG 1 cut(s) 1551
NheI GCTAGC 1 cut(s) 793
NlaIV GGNNCC 6 cut(s) 949, 2068, 2069, 2143, 2449, 2805
NmuCI GTSAC 3 cut(s) 703, 910, 1379
NsiI ATGCAT 1 cut(s) 1455
NspI RCATGY 3 cut(s) 626, 843, 2239
OliI CACNNNNGTG 1 cut(s) 2706
PaqCI CACCTGC 1 cut(s) 1478
PciI ACATGT 3 cut(s) 622, 839, 2235
PcsI WCGNNNNNNNCGW 2 cut(s) 1726, 2448
PctI GAATGC 2 cut(s) 1194, 1977
PfeI GAWTC 7 cut(s) 722, 1333, 1722, 1792, 1999, 2171, 2494
PflMI CCANNNNNTGG 1 cut(s) 2384
PinAI ACCGGT 1 cut(s) 2380
PkrI GCNGC 9 cut(s) 1038, 1099, 1486, 1543, 1767, 1941, 2535, 2729, 2808
PleI GAGTC 2 cut(s) 1099, 1182
PmaCI CACGTG 2 cut(s) 460, 920
PmlI CACGTG 2 cut(s) 460, 920
PpsI GAGTC 2 cut(s) 1099, 1182
Ppu21I YACGTR 3 cut(s) 460, 920, 1572
PpuMI RGGWCCY 2 cut(s) 488, 2067
PscI ACATGT 3 cut(s) 622, 839, 2235
Psp124BI GAGCTC 2 cut(s) 362, 2268
Psp5II RGGWCCY 2 cut(s) 488, 2067
Psp6I CCWGG 3 cut(s) 592, 765, 1403
PspCI CACGTG 2 cut(s) 460, 920
PspGI CCWGG 3 cut(s) 592, 765, 1403
PspN4I GGNNCC 6 cut(s) 949, 2068, 2069, 2143, 2449, 2805
PspPI GGNCC 7 cut(s) 184, 488, 1807, 2067, 2142, 2335, 2447
PspPPI RGGWCCY 2 cut(s) 488, 2067
PstI CTGCAG 1 cut(s) 1489
PstNI CAGNNNCTG 1 cut(s) 2598
PsuI RGATCY 3 cut(s) 947, 2288, 2314
RsaI GTAC 2 cut(s) 752, 1570
RsaNI GTAC 2 cut(s) 751, 1569
RseI CAYNNNNRTG 5 cut(s) 627, 2330, 2423, 2706, 2748
SacI GAGCTC 2 cut(s) 362, 2268
SacII CCGCGG 1 cut(s) 2810
SalI GTCGAC 1 cut(s) 2759
SatI GCNGC 9 cut(s) 1037, 1098, 1485, 1542, 1766, 1940, 2534, 2728, 2807
Sau96I GGNCC 7 cut(s) 184, 488, 1807, 2067, 2142, 2335, 2447
SchI GAGTC 2 cut(s) 1099, 1183
ScrFI CCNGG 3 cut(s) 594, 767, 1405
SduI GDGCHC 3 cut(s) 362, 2268, 2520
SexAI ACCWGGT 1 cut(s) 765
SfaNI GCATC 8 cut(s) 913, 1543, 1951, 2086, 2113, 2122, 2654, 2727
SfcI CTRYAG 1 cut(s) 1485
Sfr303I CCGCGG 1 cut(s) 2810
SgrBI CCGCGG 1 cut(s) 2810
SinI GGWCC 6 cut(s) 184, 488, 1807, 2067, 2335, 2447
SmiMI CAYNNNNRTG 5 cut(s) 627, 2330, 2423, 2706, 2748
SmlI CTYRAG 2 cut(s) 2101, 2498
SmoI CTYRAG 2 cut(s) 2101, 2498
SnaBI TACGTA 1 cut(s) 1572
SpeI ACTAGT 1 cut(s) 2747
SsiI CCGC 9 cut(s) 164, 726, 1037, 1083, 1541, 1911, 1939, 2807, 2809
SspI AATATT 1 cut(s) 2797
SspMI CTAG 6 cut(s) 794, 1985, 2136, 2571, 2738, 2748
SstI GAGCTC 2 cut(s) 362, 2268
StyD4I CCNGG 3 cut(s) 592, 765, 1403
StyI CCWWGG 1 cut(s) 2722
TaaI ACNGT 6 cut(s) 870, 955, 1248, 1800, 1952, 2391
TaiI ACGT 4 cut(s) 462, 922, 1574, 1700
TaqI TCGA 6 cut(s) 145, 1235, 1960, 1990, 2482, 2760
TatI WGTACW 1 cut(s) 750
TauI GCSGC 4 cut(s) 1039, 1544, 1942, 2809
TfiI GAWTC 7 cut(s) 722, 1333, 1722, 1792, 1999, 2171, 2494
TscAI CASTG 4 cut(s) 198, 958, 1570, 2525
TseFI GTSAC 3 cut(s) 703, 910, 1379
TseI GCWGC 5 cut(s) 1097, 1484, 1765, 2533, 2727
Tsp45I GTSAC 3 cut(s) 703, 910, 1379
TspDTI ATGAA 9 cut(s) 299, 557, 1042, 1618, 2163, 2289, 2402, 2712, 2846
TspGWI ACGGA 3 cut(s) 197, 869, 2327
TspRI CASTG 4 cut(s) 198, 958, 1570, 2525
Van91I CCANNNNNTGG 1 cut(s) 2384
VpaK11BI GGWCC 6 cut(s) 184, 488, 1807, 2067, 2335, 2447
XapI RAATTY 4 cut(s) 341, 758, 1589, 2453
XceI RCATGY 3 cut(s) 626, 843, 2239
XmiI GTMKAC 2 cut(s) 137, 2760
XspI CTAG 6 cut(s) 794, 1985, 2136, 2571, 2738, 2748
Zsp2I ATGCAT 1 cut(s) 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.