Rw0G001100

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00036
OG0000070
Physical Location & Seq
Forward (+)
90803 .. 95851
5049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G001100.1

Sequence Viewer

Length: 4092 bp
ATGGCAGTGGGAGAGATCTTTCTGGCGGCATTCCTTCAGTTGCTGCTGGACAGGTTAACGCCTCGGGAGATCCTGGGCTACTTTGGAAGCTTGGGAGGAGTTGGCAAAAAGCTCCAGAAATGGAGGGAAACACTGTCCGATATTGCAGCGGTGCTTTCCGATGCGGAGGAGAAGCAGCTCACGAACAAGGCAGTGGATCGGTGGCTGAATGACCTGAAACACTTGGCTTATGATCTGGACGATCTACTCAACACCTTTTCTAATGAGATGTTAGAACGGGAGCAGCTCAAGCTTCACCAAACTGGCACAAGCAAGGTACGAGGCTTCTTTACCAAAGTTCCTCACAAAGTTAAGTTCAATTTCAATATGAACTCCGAAGTAGACGACATTGCTAATCGGTTGCAAGACATATTTGACCGGAAAGAGAAACTCCGTTTGAAATATATTGAGCATAACTCTACTTCAACATCGTCATCGCACAGGACACCAAGTTCATATGTGTTGGATGGACCTGTGGTTGGGAGAGATGAAGACGCAGGAAAAATTGTGGAATTGTTGTCCAGAGATGCTGATCCTTCTTCTCCCACAAATTATCAAGTTGTTGCCATTGTTGGTATGGGAGGACTCGGCAAGACAACGCTTGCGGGACAAGTCTTCAGTGATGTAGCTGCAATGGAACAGTTTGATCTCAAGGCCTGGGTTTCAGTGTCTGATGACTTCGATCTTCAAACGGTGACGAGAGCTATTTTTAAGGAACTTACATCTCGGCCTTGTGATATGGATGAGTTCAGTCGACTTCAAGATAATCTGAGCAAGGAGATCGAGGGTAAAAGGTTTTTGATTGTTTTAGATGATGTCTGGAGCACGTGTGATTATGATTCATGGACAAAACTGCAAGCTCCCTTCTGTGGCGGAGCTAAGGGAAGTAAGGTAATGGTGACAACACGTGATGAAAAAGTTGCAGCATTGATGGGAGCCCCTGCTGCTGAAGTTTACTATTTAAAGACTCTATCAGATGAAGGTTGTTTGCAAGTATTTGAACAACATGTTAGCAATGACAGGCCACCGAATTTTGAGTTGCTTAAGAAGAAAATTGTTACGAACTGCAATGGATTGCCATTGGCTGCAAAAACTCTTGGTGGTGTTTTACGTTGTAAAGAAACTGACAAGTGGGAGGAAATACTGAATGACAAATTATGGAGTATATCAGATGGGACTCATGGGAGTAAAATACTCCCTGTACTGAGATTGAGTTATCATTATCTGCCTTTAACTTTGAAGAGGTGCTTTGCCTATTGCTCAATACTTCCCAATGACTATGAATTTGGGAAAACCCAATTGATCCTTCTGTGGATGGCAGAGGGTTTCCTTGAGCAACCAGAAGGGACTAAAGCAATGGAAGATATCGGTGACGAATATTTTGGGGAGCTATTGTCTCGGTCATTATTTCAAAAGGCGGGCAAAAACAGTTTGTGCTATGTAATGCATGACCTTGTTGGTGATTTGGCACGATGGGCTGCTGGAGACACATTTTGCAGATTGGAGGATAAACTGCATGGTAGATGTTCTGCAAAGATTCGCCATCTGGCTTACATTTCTAGTAAGTTCGATGGGGTAAAAAGATTTGAGACATTTTCTGAAGCAAAACGCTTGCGGACTTTCCTACCACTTCCAGTTTCCGGTGGTTATGAGAATTATCTAACTCGTTATGCTACTTTAGATTTATTGCCACAATTGAAGTACTTGCGGGTGCTCTCCTTCAATGGCTATAAACTGACCGAGGTGCCGAATTCCGTAGGTAAGTTGAGACATCTACGGTACCTTGATCTTTCTCACACATTAATAACGAGTTTGCCTGAGTCAACCTGCATGCTTTACAACTTACAGACGTTAATATTAGAGAATTGTTCAAAATTGAAGACCCTACCTTCAAACATGAGCAATTTATCTAATTTACGTCATCTCAACAATTCAAACATTCCTTCATTGGAAGAAATGCCTCCCCAAGTAAGTCGGTTGATTCATCTGCGAACTTTGCCTGATTTTGTGGTGGGGAAAGGTAGTGTATCCAGGATTGGAGAGATAGGGTCATTGCATCTTTGTGGAACCTTGAGGGTTGGAAGATTGGAAAATGTGATTGATGTTGGGGATGCGAAGAGGGCCCAAATAATGAACAAGGAAGGGTTGAATACCTTGCAATTGGAATGGAGTGGCGCGAGTGAGAGGGAGTCGGAGGTGCTCTGTAGTTTAGAACCTCATAAAAAGCTTCTAGACCTCACCATCAAGGGCTACAATGGTTTCGAATTTTCAAAATGGATTGGACATCCTTCATTCTCTGATATGACGCATGTGAAGTTAGAGAATTGTAAAAATTGTCAATTCTTACCTCCTCTTGGCCAATTACCTTTTCTGAAAGGACTTTGGATACAAGGACTGGCCAATGTTGAAAGTGTTGGTGCTGAGTTTTATGGAGAGTGCAGCTTGCCTTTTCCAGTGTTGGAAGACATGAGCTTCGAGGATATGCAAAGTTGGAAGGCGTGGCTTCCTTGCAAAAGAAATGAAGAAATTCGAGTTTTCCCTTGCCTGCGAAAGCTTACCATTGTTAAATGCCCCAAATTGAAAGGTTGGTTGCCCAAAAACATGGATTCATTATCAGTGCTGTGTATTCTTGCATGTGAAGAGTTAACGGTTTCAATTGCCAATTATAAACGTCTACGTCTGTTGACCATCAATGGTTGTAAAAGGGTAGTGCACAGAAGTGGAGTTAAGTTTGAGTCACGGGCTGATATTTCAGAGTTCGTACTCCATATAGCCGGGTTCATCAGTGGATTGCTAAAGCTTCAAGGATTGGGGATTGCTGATTGTGAAGAGTTGACATGTTTATGGGAGAATGAGGATAGATGGCTGCAACGTCGGATTTCTAATATTATTGGAGGCTACATCTCTGACACCCCCTCTCACTTTATTCTAGAGCTAAGAGCACTAAAACAACTTGGGCTAACTGGATGCTCAAATCTAATTTCTTTTCCAGAAGCTGGTTTGCCACCTTGTCTTGAACGTGTAAGTATGGAGTCGTGTAATTCTTTGACATATTTCATAAGGTATCAAGTACCGCCAAGCATAAGAAGAATAGAGATAGCAAAATGTCGAAATTTGAAACTATTAGTGAATGATGCTGGGGAGCTAGAGGGTTGTCTGGAGAATTTGAAAATAACAGAGTGCGCATCTTTGACATCTTTGACTTCAGGCAAAGGAGACCGACTACCCAGAACGCTCAAATATCTTCATTTAAGTGACTGTCAACAATTGGAGTCAATAAGCAATACATTCCACGATGACACTTGTCTTGAAGATCTTAGGATAGAGAGGTGTGCAAATCTCAAATCCTTACCAGAGGGGCTATGCCATCTCTCCGCTCTTTGCATTTTAATTATACGCAATTGTGGAAGTCTCGTTTCCTTCCCGGGAGGAGGGTTGCCATCCAACCTGATAGACTTTCGTATCTACAATTGTGATCAATTGGAAGCCCTCCCCAGAGGCATGGACAACCTCAGCTCTCTTCAGACTTTGGTGTTGAGATACTGTGGAGGTTTGGCATCCATTATAGAAGAGGGTTTCCCACCCAACCTAATTAATCTTCGTATTGGCAATCGCAAAAGCTGCAAGCCTCTCTCAGAGTGGGGGCTTCACCACTTGGACAGACTCACCTCTCTTAAAGGCTTGTGGATCCATGGTGTAGATCCAGATCTGGCGTCCTTTCCGCCAAAGGAGGTGCTGCTCCCTAAATCTCTCATTCAACTCGACATTGGAGAGTTCCCAAATCTGAAGCACCTGTCATCATCTTTTCAATCACTCTCCTCTCTTGAATCCCTTAACATTTGGGATTGCCCAAAGCTAGCATCTATTATTCCAGAAAAAGAGGATCATCTGCCTCTTTCACTTACACAGCTTCGCATCTTTGAGTTATGTCCGCTGCTCACAAAGAAATACCAACCAGGTAAAGGACGACACTGGCCCAAAGAAATAGCCCACATCCCTTACGTTTATTTTGGAGACTGGGAGGACGAAGCCAAAGCCGACCGCGGTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1363

Amino Acids

153.49

Weight (kDa)

6.57

Isoelectric Point (pI)

43.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 99 1.9e-18 Rx N-terminal domain
NB-ARC PF00931 180 - 352 8e-38 NB-ARC domain
WHD_DRP PF23559 435 - 503 7.9e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 576 - 847 2e-23 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 693 - 811 2.6e-31 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2706
Acc16I TGCGCA 1 cut(s) 3223
Acc36I ACCTGC 1 cut(s) 1874
Acc65I GGTACC 1 cut(s) 1818
AccB1I GGYRCC 2 cut(s) 1783, 1818
AccB7I CCANNNNNTGG 1 cut(s) 3035
AccBSI CCGCTC 1 cut(s) 3416
AccI GTMKAC 3 cut(s) 381, 793, 2713
AccII CGCG 2 cut(s) 2216, 4083
AclWI GGATC 8 cut(s) 64, 204, 566, 1336, 3721, 3734, 3734, 3930
AcoI YGGCCR 2 cut(s) 2395, 2436
AcsI RAATTY 7 cut(s) 1069, 1322, 1789, 2303, 2565, 3151, 3202
AcuI CTGAAG 7 cut(s) 20, 640, 1008, 1659, 3228, 3545, 3845
AcvI CACGTG 2 cut(s) 867, 947
AcyI GRCGYC 1 cut(s) 3752
AfaI GTAC 6 cut(s) 318, 1242, 1742, 1820, 2802, 3111
AfiI CCNNNNNNNGG 7 cut(s) 518, 908, 1679, 2393, 3035, 3470, 4001
AflII CTTAAG 1 cut(s) 1082
AflIII ACRYGT 5 cut(s) 866, 944, 1045, 2876, 3058
AjnI CCWGG 4 cut(s) 72, 695, 2069, 3994
AjuI GAANNNNNNNTTGG 4 cut(s) 1329, 1361, 2391, 2423
AleI CACNNNNGTG 1 cut(s) 2757
AloI GAACNNNNNNTCC 2 cut(s) 475, 507
Alw21I GWGCWC 5 cut(s) 866, 1755, 2241, 2754, 2983
Alw26I GTCTC 7 cut(s) 1440, 1518, 1622, 1801, 3249, 3455, 4047
Alw44I GTGCAC 1 cut(s) 2750
AlwI GGATC 8 cut(s) 64, 204, 566, 1336, 3721, 3734, 3734, 3930
AlwNI CAGNNNCTG 3 cut(s) 43, 710, 3035
Ama87I CYCGRG 2 cut(s) 63, 3463
AoxI GGCC 7 cut(s) 693, 767, 1061, 2160, 2395, 2436, 4013
ApaI GGGCCC 1 cut(s) 2164
ApaLI GTGCAC 1 cut(s) 2750
ApoI RAATTY 7 cut(s) 1069, 1322, 1789, 2303, 2565, 3151, 3202
ArsI GACNNNNNNTTYG 6 cut(s) 1307, 1339, 1403, 1435, 2495, 2527
AseI ATTAAT 2 cut(s) 1841, 3633
Asp700I GAANNNNTTC 1 cut(s) 2565
Asp718I GGTACC 1 cut(s) 1818
AspLEI GCGC 2 cut(s) 2216, 3224
AspS9I GGNCC 4 cut(s) 509, 2160, 2161, 4014
AsuC2I CCSGG 3 cut(s) 2815, 3464, 3465
AsuHPI GGTGA 8 cut(s) 287, 745, 949, 1421, 1511, 2269, 3680, 3697
AsuII TTCGAA 1 cut(s) 2301
AsuNHI GCTAGC 1 cut(s) 3895
AvaI CYCGRG 2 cut(s) 63, 3463
AvaII GGWCC 1 cut(s) 509
BaeGI GKGCMC 2 cut(s) 2164, 2754
BaeI ACNNNNGTAYC 2 cut(s) 3484, 3517
BalI TGGCCA 2 cut(s) 2397, 2438
BamHI GGATCC 1 cut(s) 3726
BanI GGYRCC 2 cut(s) 1783, 1818
BanII GRGCYC 2 cut(s) 979, 2164
BbrPI CACGTG 2 cut(s) 867, 947
BbsI GAAGAC 4 cut(s) 537, 646, 1925, 2508
Bbv12I GWGCWC 5 cut(s) 866, 1755, 2241, 2754, 2983
BbvCI CCTCAGC 1 cut(s) 3551
BcgI CGANNNNNNTGC 2 cut(s) 802, 836
BciT130I CCWGG 4 cut(s) 74, 697, 2071, 3996
BciVI GTATCC 2 cut(s) 2077, 2418
BclI TGATCA 1 cut(s) 3514
BcnI CCSGG 3 cut(s) 2815, 3464, 3465
BcoDI GTCTC 7 cut(s) 1440, 1518, 1622, 1801, 3249, 3455, 4047
BfaI CTAG 5 cut(s) 1599, 2270, 2969, 3185, 3896
BfmI CTRYAG 1 cut(s) 2242
BfrI CTTAAG 1 cut(s) 1082
BfuAI ACCTGC 1 cut(s) 1874
BfuI GTATCC 2 cut(s) 2077, 2418
BglII AGATCT 3 cut(s) 15, 3352, 3745
BmcAI AGTACT 1 cut(s) 1742
Bme1390I CCNGG 7 cut(s) 74, 697, 2071, 2815, 3464, 3465, 3996
Bme18I GGWCC 1 cut(s) 509
BmeT110I CYCGRG 2 cut(s) 63, 3463
BmgT120I GGNCC 4 cut(s) 509, 2160, 2161, 4014
BmiI GGNNCC 6 cut(s) 976, 1785, 1820, 2107, 2162, 3728
BmrFI CCNGG 7 cut(s) 74, 697, 2071, 2815, 3464, 3465, 3996
BmrI ACTGGG 1 cut(s) 4066
BmtI GCTAGC 1 cut(s) 3899
BmuI ACTGGG 1 cut(s) 4066
BoxI GACNNNNGTC 1 cut(s) 3342
BpiI GAAGAC 4 cut(s) 537, 646, 1925, 2508
BplI GAGNNNNNCTC 2 cut(s) 440, 472
BpmI CTGGAG 4 cut(s) 98, 880, 1542, 3218
Bpu10I CCTNAGC 2 cut(s) 918, 3551
Bpu14I TTCGAA 1 cut(s) 2301
BpuEI CTTGAG 4 cut(s) 272, 674, 1391, 2131
BpuMI CCSGG 3 cut(s) 2815, 3464, 3465
BsaAI YACGTR 2 cut(s) 867, 947
BsaBI GATNNNNATC 2 cut(s) 570, 3744
BsaHI GRCGYC 1 cut(s) 3752
BsaI GGTCTC 1 cut(s) 3249
BsaJI CCNNGG 7 cut(s) 62, 73, 696, 1779, 3463, 3730, 4081
BsaWI WCCGGW 2 cut(s) 417, 1679
BsaXI ACNNNNNCTCC 4 cut(s) 853, 883, 1418, 1448
Bsc4I CCNNNNNNNGG 7 cut(s) 518, 908, 1679, 2393, 3035, 3470, 4001
Bse1I ACTGG 7 cut(s) 307, 1673, 2439, 2492, 3007, 4016, 4061
Bse3DI GCAATG 6 cut(s) 387, 678, 1060, 1114, 1401, 2090
Bse8I GATNNNNATC 2 cut(s) 570, 3744
BseBI CCWGG 4 cut(s) 74, 697, 2071, 3996
BseDI CCNNGG 7 cut(s) 62, 73, 696, 1779, 3463, 3730, 4081
BseGI GGATG 9 cut(s) 511, 787, 1359, 2155, 2323, 3011, 3479, 3596, 4032
BseJI GATNNNNATC 2 cut(s) 570, 3744
BseLI CCNNNNNNNGG 7 cut(s) 518, 908, 1679, 2393, 3035, 3470, 4001
BseMI GCAATG 6 cut(s) 387, 678, 1060, 1114, 1401, 2090
BseMII CTCAG 6 cut(s) 800, 1235, 1848, 2451, 3565, 3687
BseNI ACTGG 7 cut(s) 307, 1673, 2439, 2492, 3007, 4016, 4061
BseRI GAGGAG 5 cut(s) 111, 182, 2379, 3483, 3847
BseSI GKGCMC 2 cut(s) 2164, 2754
BseYI CCCAGC 1 cut(s) 3176
BsgI GTGCAG 1 cut(s) 2497
Bsh1236I CGCG 2 cut(s) 2216, 4083
Bsh1285I CGRYCG 1 cut(s) 4081
BshFI GGCC 7 cut(s) 695, 769, 1063, 2162, 2397, 2438, 4015
BshNI GGYRCC 2 cut(s) 1783, 1818
BsiEI CGRYCG 1 cut(s) 4081
BsiHKAI GWGCWC 5 cut(s) 866, 1755, 2241, 2754, 2983
BsiHKCI CYCGRG 2 cut(s) 63, 3463
BsiSI CCGG 4 cut(s) 418, 1680, 2814, 3464
BslFI GGGAC 3 cut(s) 660, 1228, 1399
BslI CCNNNNNNNGG 7 cut(s) 518, 908, 1679, 2393, 3035, 3470, 4001
BsmAI GTCTC 7 cut(s) 1440, 1518, 1622, 1801, 3249, 3455, 4047
BsmFI GGGAC 3 cut(s) 660, 1228, 1399
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 7 cut(s) 695, 769, 1063, 2162, 2397, 2438, 4015
Bso31I GGTCTC 1 cut(s) 3249
BsoBI CYCGRG 2 cut(s) 63, 3463
Bsp119I TTCGAA 1 cut(s) 2301
Bsp120I GGGCCC 1 cut(s) 2160
Bsp1286I GDGCHC 7 cut(s) 866, 979, 1755, 2164, 2241, 2754, 2983
Bsp19I CCATGG 1 cut(s) 3730
BspANI GGCC 7 cut(s) 695, 769, 1063, 2162, 2397, 2438, 4015
BspCNI CTCAG 6 cut(s) 801, 1236, 1849, 2452, 3564, 3686
BspFNI CGCG 2 cut(s) 2216, 4083
BspLI GGNNCC 6 cut(s) 976, 1785, 1820, 2107, 2162, 3728
BspMI ACCTGC 1 cut(s) 1874
BspOI GCTAGC 1 cut(s) 3899
BspPI GGATC 8 cut(s) 64, 204, 566, 1336, 3721, 3734, 3734, 3930
BspT104I TTCGAA 1 cut(s) 2301
BspT107I GGYRCC 2 cut(s) 1783, 1818
BspTI CTTAAG 1 cut(s) 1082
BspTNI GGTCTC 1 cut(s) 3249
BsrBI CCGCTC 1 cut(s) 3416
BsrDI GCAATG 6 cut(s) 387, 678, 1060, 1114, 1401, 2090
BsrI ACTGG 7 cut(s) 307, 1673, 2439, 2492, 3007, 4016, 4061
BssECI CCNNGG 7 cut(s) 62, 73, 696, 1779, 3463, 3730, 4081
BssNI GRCGYC 1 cut(s) 3752
BssT1I CCWWGG 1 cut(s) 3730
Bst2UI CCWGG 4 cut(s) 74, 697, 2071, 3996
Bst4CI ACNGT 8 cut(s) 135, 681, 733, 1469, 1818, 2689, 3299, 3584
Bst6I CTCTTC 6 cut(s) 1274, 2150, 2673, 2862, 3564, 3603
BstACI GRCGYC 1 cut(s) 3752
BstAFI CTTAAG 1 cut(s) 1082
BstAPI GCANNNNNTGC 1 cut(s) 3660
BstBAI YACGTR 2 cut(s) 867, 947
BstBI TTCGAA 1 cut(s) 2301
BstC8I GCNNGC 9 cut(s) 642, 897, 1459, 1652, 1871, 2483, 2585, 3665, 3897
BstDEI CTNAG 9 cut(s) 809, 918, 1244, 1857, 2460, 2975, 3356, 3551, 3673
BstDSI CCRYGG 2 cut(s) 3730, 4081
BstF5I GGATG 9 cut(s) 511, 787, 1359, 2155, 2323, 3011, 3479, 3596, 4032
BstFNI CGCG 2 cut(s) 2216, 4083
BstHHI GCGC 2 cut(s) 2216, 3224
BstMAI GTCTC 7 cut(s) 1440, 1518, 1622, 1801, 3249, 3455, 4047
BstMCI CGRYCG 1 cut(s) 4081
BstMWI GCNNNNNNNGC 6 cut(s) 289, 983, 1514, 2035, 2159, 3660
BstNI CCWGG 4 cut(s) 74, 697, 2071, 3996
BstNSI RCATGY 5 cut(s) 1049, 1873, 2351, 2676, 2880
BstPAI GACNNNNGTC 1 cut(s) 3342
BstSCI CCNGG 7 cut(s) 72, 695, 2069, 2813, 3462, 3463, 3994
BstSFI CTRYAG 1 cut(s) 2242
BstSLI GKGCMC 2 cut(s) 2164, 2754
BstUI CGCG 2 cut(s) 2216, 4083
BstV2I GAAGAC 4 cut(s) 537, 646, 1925, 2508
BstX2I RGATCY 6 cut(s) 15, 69, 3352, 3726, 3739, 3745
BstXI CCANNNNNNTGG 2 cut(s) 2641, 3541
BstYI RGATCY 6 cut(s) 15, 69, 3352, 3726, 3739, 3745
BsuI GTATCC 2 cut(s) 2077, 2418
BsuRI GGCC 7 cut(s) 695, 769, 1063, 2162, 2397, 2438, 4015
BtgI CCRYGG 2 cut(s) 3730, 4081
BtgZI GCGATG 1 cut(s) 459
BtsCI GGATG 9 cut(s) 511, 787, 1359, 2155, 2323, 3011, 3479, 3596, 4032
BtsI GCAGTG 2 cut(s) 12, 198
BtsIMutI CAGTG 9 cut(s) 12, 131, 198, 664, 711, 2499, 2661, 2830, 4009
BveI ACCTGC 1 cut(s) 1874
Cac8I GCNNGC 9 cut(s) 642, 897, 1459, 1652, 1871, 2483, 2585, 3665, 3897
CaiI CAGNNNCTG 3 cut(s) 43, 710, 3035
CfoI GCGC 2 cut(s) 2216, 3224
Cfr13I GGNCC 4 cut(s) 509, 2160, 2161, 4014
Cfr42I CCGCGG 1 cut(s) 4084
Cfr9I CCCGGG 1 cut(s) 3463
CseI GACGC 3 cut(s) 542, 2353, 3741
CsiI ACCWGGT 1 cut(s) 3994
Csp6I GTAC 6 cut(s) 317, 1241, 1741, 1819, 2801, 3110
CviQI GTAC 6 cut(s) 317, 1241, 1741, 1819, 2801, 3110
DdeI CTNAG 9 cut(s) 809, 918, 1244, 1857, 2460, 2975, 3356, 3551, 3673
DraI TTTAAA 1 cut(s) 1002
EaeI YGGCCR 2 cut(s) 2395, 2436
Eam1104I CTCTTC 6 cut(s) 1274, 2150, 2673, 2862, 3564, 3603
EarI CTCTTC 6 cut(s) 1274, 2150, 2673, 2862, 3564, 3603
EciI GGCGGA 2 cut(s) 927, 3750
Eco130I CCWWGG 1 cut(s) 3730
Eco147I AGGCCT 1 cut(s) 695
Eco24I GRGCYC 2 cut(s) 979, 2164
Eco31I GGTCTC 1 cut(s) 3249
Eco32I GATATC 1 cut(s) 1405
Eco47I GGWCC 1 cut(s) 509
Eco57I CTGAAG 7 cut(s) 20, 640, 1008, 1659, 3228, 3545, 3845
Eco72I CACGTG 2 cut(s) 867, 947
Eco88I CYCGRG 2 cut(s) 63, 3463
EcoO109I RGGNCCY 1 cut(s) 2160
EcoRI GAATTC 1 cut(s) 1789
EcoRII CCWGG 4 cut(s) 72, 695, 2069, 3994
EcoRV GATATC 1 cut(s) 1405
EcoT14I CCWWGG 1 cut(s) 3730
EcoT22I ATGCAT 1 cut(s) 1488
EcoT38I GRGCYC 2 cut(s) 979, 2164
ErhI CCWWGG 1 cut(s) 3730
FalI AAGNNNNNCTT 2 cut(s) 1271, 1303
FaqI GGGAC 3 cut(s) 660, 1228, 1399
FauI CCCGC 3 cut(s) 637, 1450, 1740
FauNDI CATATG 1 cut(s) 496
FbaI TGATCA 1 cut(s) 3514
FblI GTMKAC 3 cut(s) 381, 793, 2713
FokI GGATG 9 cut(s) 518, 794, 1366, 2162, 2310, 3018, 3466, 3583, 4019
FriOI GRGCYC 2 cut(s) 979, 2164
FspAI RTGCGCAY 1 cut(s) 3223
FspBI CTAG 5 cut(s) 1599, 2270, 2969, 3185, 3896
FspI TGCGCA 1 cut(s) 3223
GlaI GCGC 2 cut(s) 2215, 3223
GsaI CCCAGC 1 cut(s) 3180
GsuI CTGGAG 4 cut(s) 98, 880, 1542, 3218
HaeIII GGCC 7 cut(s) 695, 769, 1063, 2162, 2397, 2438, 4015
HapII CCGG 4 cut(s) 418, 1680, 2814, 3464
HgaI GACGC 3 cut(s) 542, 2353, 3741
HhaI GCGC 2 cut(s) 2216, 3224
Hin1I GRCGYC 1 cut(s) 3752
Hin6I GCGC 2 cut(s) 2214, 3222
HinP1I GCGC 2 cut(s) 2214, 3222
HincII GTYRAC 7 cut(s) 57, 794, 1863, 2685, 2724, 2874, 3302
HindII GTYRAC 7 cut(s) 57, 794, 1863, 2685, 2724, 2874, 3302
HindIII AAGCTT 5 cut(s) 88, 290, 2264, 2591, 2837
HpaI GTTAAC 2 cut(s) 57, 2685
HpaII CCGG 4 cut(s) 418, 1680, 2814, 3464
HphI GGTGA 8 cut(s) 287, 745, 949, 1421, 1511, 2269, 3680, 3697
Hpy99I CGWCG 1 cut(s) 2916
HpyCH4III ACNGT 8 cut(s) 135, 681, 733, 1469, 1818, 2689, 3299, 3584
HpyF10VI GCNNNNNNNGC 6 cut(s) 289, 983, 1514, 2035, 2159, 3660
HpyF3I CTNAG 9 cut(s) 809, 918, 1244, 1857, 2460, 2975, 3356, 3551, 3673
Hsp92I GRCGYC 1 cut(s) 3752
HspAI GCGC 2 cut(s) 2214, 3222
KpnI GGTACC 1 cut(s) 1822
Ksp22I TGATCA 1 cut(s) 3514
KspAI GTTAAC 2 cut(s) 57, 2685
KspI CCGCGG 1 cut(s) 4084
LmnI GCTCC 9 cut(s) 117, 280, 861, 904, 914, 974, 1426, 3181, 3783
MabI ACCWGGT 1 cut(s) 3994
MaeI CTAG 5 cut(s) 1599, 2270, 2969, 3185, 3896
MaeIII GTNAC 6 cut(s) 733, 937, 1096, 1409, 2775, 3293
MbiI CCGCTC 1 cut(s) 3416
MfeI CAATTG 8 cut(s) 1337, 1733, 2198, 2694, 3305, 3439, 3508, 3518
MflI RGATCY 6 cut(s) 15, 69, 3352, 3726, 3739, 3745
MhlI GDGCHC 7 cut(s) 866, 979, 1755, 2164, 2241, 2754, 2983
MlsI TGGCCA 2 cut(s) 2397, 2438
MluNI TGGCCA 2 cut(s) 2397, 2438
MlyI GAGTC 9 cut(s) 618, 1000, 1210, 1868, 2237, 2783, 3080, 3320, 3696
MmeI TCCRAC 7 cut(s) 483, 2098, 2211, 2478, 2510, 2894, 3507
Mox20I TGGCCA 2 cut(s) 2397, 2438
Mph1103I ATGCAT 1 cut(s) 1488
MroXI GAANNNNTTC 1 cut(s) 2565
MscI TGGCCA 2 cut(s) 2397, 2438
MslI CAYNNNNRTG 4 cut(s) 2100, 2757, 2881, 3291
Msp20I TGGCCA 2 cut(s) 2397, 2438
MspA1I CMGCKG 3 cut(s) 149, 3973, 4083
MspCI CTTAAG 1 cut(s) 1082
MspI CCGG 4 cut(s) 418, 1680, 2814, 3464
MspR9I CCNGG 7 cut(s) 74, 697, 2071, 2815, 3464, 3465, 3996
MunI CAATTG 8 cut(s) 1337, 1733, 2198, 2694, 3305, 3439, 3508, 3518
Mva1269I GAATGC 1 cut(s) 29
MvaI CCWGG 4 cut(s) 74, 697, 2071, 3996
MvnI CGCG 2 cut(s) 2216, 4083
MwoI GCNNNNNNNGC 6 cut(s) 289, 983, 1514, 2035, 2159, 3660
NciI CCSGG 3 cut(s) 2815, 3464, 3465
NcoI CCATGG 1 cut(s) 3730
NdeI CATATG 1 cut(s) 496
NheI GCTAGC 1 cut(s) 3895
NlaIV GGNNCC 6 cut(s) 976, 1785, 1820, 2107, 2162, 3728
NmeAIII GCCGAG 2 cut(s) 606, 745
NmuCI GTSAC 5 cut(s) 733, 937, 1409, 2775, 3293
NsbI TGCGCA 1 cut(s) 3223
NsiI ATGCAT 1 cut(s) 1488
NspI RCATGY 5 cut(s) 1049, 1873, 2351, 2676, 2880
NspV TTCGAA 1 cut(s) 2301
OliI CACNNNNGTG 1 cut(s) 2757
PaeI GCATGC 1 cut(s) 1873
PceI AGGCCT 1 cut(s) 695
PciI ACATGT 2 cut(s) 1045, 2876
PctI GAATGC 1 cut(s) 29
PdmI GAANNNNTTC 1 cut(s) 2565
PfeI GAWTC 5 cut(s) 878, 1576, 2020, 2645, 3866
PflMI CCANNNNNTGG 1 cut(s) 3035
PfoI TCCNGGA 1 cut(s) 2069
PleI GAGTC 9 cut(s) 618, 1000, 1210, 1867, 2236, 2782, 3079, 3319, 3696
PmaCI CACGTG 2 cut(s) 867, 947
PmlI CACGTG 2 cut(s) 867, 947
PpsI GAGTC 9 cut(s) 618, 1000, 1210, 1867, 2236, 2782, 3079, 3319, 3696
Ppu21I YACGTR 2 cut(s) 867, 947
PscI ACATGT 2 cut(s) 1045, 2876
PshAI GACNNNNGTC 1 cut(s) 3342
PshBI ATTAAT 2 cut(s) 1841, 3633
PsiI TTATAA 1 cut(s) 2706
Psp6I CCWGG 4 cut(s) 72, 695, 2069, 3994
PspCI CACGTG 2 cut(s) 867, 947
PspFI CCCAGC 1 cut(s) 3176
PspGI CCWGG 4 cut(s) 72, 695, 2069, 3994
PspN4I GGNNCC 6 cut(s) 976, 1785, 1820, 2107, 2162, 3728
PspOMI GGGCCC 1 cut(s) 2160
PspPI GGNCC 4 cut(s) 509, 2160, 2161, 4014
PstNI CAGNNNCTG 3 cut(s) 43, 710, 3035
PsuI RGATCY 6 cut(s) 15, 69, 3352, 3726, 3739, 3745
RsaI GTAC 6 cut(s) 318, 1242, 1742, 1820, 2802, 3111
RsaNI GTAC 6 cut(s) 317, 1241, 1741, 1819, 2801, 3110
RseI CAYNNNNRTG 4 cut(s) 2100, 2757, 2881, 3291
SacII CCGCGG 1 cut(s) 4084
SalI GTCGAC 1 cut(s) 792
Sau96I GGNCC 4 cut(s) 509, 2160, 2161, 4014
ScaI AGTACT 1 cut(s) 1742
SchI GAGTC 9 cut(s) 618, 1000, 1210, 1868, 2237, 2783, 3080, 3320, 3696
ScrFI CCNGG 7 cut(s) 74, 697, 2071, 2815, 3464, 3465, 3996
SduI GDGCHC 7 cut(s) 866, 979, 1755, 2164, 2241, 2754, 2983
SexAI ACCWGGT 1 cut(s) 3994
SfcI CTRYAG 1 cut(s) 2242
Sfr303I CCGCGG 1 cut(s) 4084
SfuI TTCGAA 1 cut(s) 2301
SgrBI CCGCGG 1 cut(s) 4084
SinI GGWCC 1 cut(s) 509
SmaI CCCGGG 1 cut(s) 3465
SmiMI CAYNNNNRTG 4 cut(s) 2100, 2757, 2881, 3291
SmlI CTYRAG 5 cut(s) 287, 689, 1082, 1370, 2110
SmoI CTYRAG 5 cut(s) 287, 689, 1082, 1370, 2110
SphI GCATGC 1 cut(s) 1873
SseBI AGGCCT 1 cut(s) 695
SspI AATATT 3 cut(s) 1418, 1896, 2926
SspMI CTAG 5 cut(s) 1599, 2270, 2969, 3185, 3896
StuI AGGCCT 1 cut(s) 695
StyD4I CCNGG 7 cut(s) 72, 695, 2069, 2813, 3462, 3463, 3994
StyI CCWWGG 1 cut(s) 3730
TaaI ACNGT 8 cut(s) 135, 681, 733, 1469, 1818, 2689, 3299, 3584
TaqI TCGA 9 cut(s) 720, 793, 822, 1608, 2301, 2514, 2569, 3148, 3801
TaqII GACCGA 3 cut(s) 1428, 1793, 3273
TatI WGTACW 2 cut(s) 1240, 1740
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 5 cut(s) 878, 1576, 2020, 2645, 3866
TscAI CASTG 9 cut(s) 12, 138, 198, 664, 711, 2499, 2661, 2830, 4016
TseFI GTSAC 5 cut(s) 733, 937, 1409, 2775, 3293
Tsp45I GTSAC 5 cut(s) 733, 937, 1409, 2775, 3293
TspGWI ACGGA 2 cut(s) 422, 1783
TspMI CCCGGG 1 cut(s) 3463
TspRI CASTG 9 cut(s) 12, 138, 198, 664, 711, 2499, 2661, 2830, 4016
Van91I CCANNNNNTGG 1 cut(s) 3035
Vha464I CTTAAG 1 cut(s) 1082
VneI GTGCAC 1 cut(s) 2750
VpaK11BI GGWCC 1 cut(s) 509
VspI ATTAAT 2 cut(s) 1841, 3633
XapI RAATTY 7 cut(s) 1069, 1322, 1789, 2303, 2565, 3151, 3202
XbaI TCTAGA 2 cut(s) 2269, 2968
XceI RCATGY 5 cut(s) 1049, 1873, 2351, 2676, 2880
XcmI CCANNNNNNNNNTGG 1 cut(s) 613
XmaI CCCGGG 1 cut(s) 3463
XmiI GTMKAC 3 cut(s) 381, 793, 2713
XmnI GAANNNNTTC 1 cut(s) 2565
XspI CTAG 5 cut(s) 1599, 2270, 2969, 3185, 3896
ZrmI AGTACT 1 cut(s) 1742
Zsp2I ATGCAT 1 cut(s) 1488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.