FvH4_7g27642

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000070
Physical Location & Seq
Reverse (-)
20604986 .. 20608942
3957 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g27642.t1

Sequence Viewer

Length: 3957 bp
ATGCTGAAGCGGAGGATAGAAAGACAACAAGGAAGCAAAAGCAAGTTATGGAGCTCACTCTCTAAAGTTAAGTCCAATTTTGATCTGAACTCAGCAATCAAGAAGATAAGTGATCGACTTGTGGAGATAACTACACGAGGAAAGCAGTTTGATTTGCAGAAACTTGGAGTGTCTAAAAAGCCATGGAAAATGCCACCCACTACTTATCAACTTGATGGACCTGTGATCGGAAGGGATGAAGCAAAGGCTAAGATTCTCTATGATTTGCTTTCAAAACAAGAGAATTATCATGTGGTTGGTATTGTCGGTACTGGAGGACTAGGAAAGACAACACTTGCTAAACATGTATTCAACGATGCTGCAACTGGCCAGTTTTCCCCAAAAGGATGGGTATCTGTGTCAGACGACTTTGACATTGTAAGGGTGGCAGCAGCCGTTTTGGAATCAGTCACGTCCTCTCCAGTTCAGGAATTGAAGGAGCTTAACTCAATTCTGGAGAAGTTAAGTAAGGAATTGGCTGGCAAGAAGTTTTTAATTGTTCTTGATGATGTCTGGCACACTTGTAGCTATGGTCAATGGACTACACTGCAGGCCTCCTTTCGTGTTGGAGCAGCTGGAAGTAAGATCATCGTGACAACACGTGACGCCAATGTCGCAAATATGATGGGAGATAGTAGCCCTTATAACTTGAACCCGATGTCAGAAGATGATTGTTGGGAACTCTTTCAGCACCATGCACAGTTAAAAAACAGACCTGAAGTTGTTGAGTTCCTTAAAGAGAAAATTGTACAAAAATGCAATGGGTTACCGTTAGTTGCAAGGACTCTTGGTGGGCTTTTACGTTGTAAAGAAGTAGACGAATGGGAAGACATATTAGACCACAAGATGAGGACTCTATCAGATCAGAACAAGAAGGAAATCCTTCCAGCATTGAAACTGAGCTATCATTATCTCCCTTCGACTTTGAAACGGTGTTTCTCCTACTGTGGAATACTTCCAAATGATTATGAATTTGAGGAGATGCAATTGATCCTTTGGTGGATGGCAGAAGGTTTGATTCCGCCCAAAGAACATGAGCAAATGGAAGATTTGGGACGTGACTATTTTCAAGAGTTGGTGTCCAGGTCATTATTTCAAAAATCAAGCAAAACAGAGTCGAAATACATAATGCATGACCTGGTTACTGAATTGGCACGTTGGGCTGCAGGAAGCTCATGTTCTAGATTGGAGGATATGCAGAATTATGACTCGCAGCAACGTACATGTTTGCCCAAGGTTCGTCATTCGTCTTACATTCTTGGTGCGTATGATGGGGTTAAGAAGTTCGAGGTCTATTCTGAAGCTACATCTTTGCGAACGTTCTTACCACTTTCACCTTCATATGGTCATTATCTGGCTCATAAGGTTACTTCTGATTTATTTCCCAAACTCCATTACTTACGACTCCTCTCTTTGAAAAGTTATCAAATAATCGAGTTGCCGAATACAATCAGTGAATTGAAGCATCTACGTTATCTTGATCTCTCCCACACTCGGATAAGAAGTTTGCCAGACTCGACAACAACTCTGTACAACTTGCAGACATTGTTATTGAAAAGTTGTGACAAGTTGAAGGCACTGCCCACAAGCATGAGAAACCTAGTTAATTTGCGTCATCTCAACAATTCAGGTACAGATTCATTGGAAGAAATGCCTCCTCAGCTTGGTCAATTGACTAACCTCCAAACATTGCCTGAGTTTGTTATTGGCAAAGGTAGTGGATCAGGAGTAAGAGAGATTGAGTCATTATTGCATCTGCAAGGGACATTGCACATCTCAAGACTAGAAAATGTGATTTGTGTCGAGGATGCCAGGAGTGCCAACTTAAAGAGCAAGGAAAGGCTTGAAGCCCTATTTCTTGAATGGTCTTCTTCGAGTGTCTCAACAGAAGATGCAGCAATTGTGCTTGGCATGTTACAACCTCATAGCAAGCTCAAAAATCTCACCATCAAAGGTTATGGTGGATTGAAATTTTCAACATGGATTGGGAATCCTTCATTCACTAAAATGGTGCGGGTAAGGTTAGAAGGTTGTAATCATTGTCAATTCTTGCCACCATTCGGACAGTTGCCTTGTCTCAAAGAACTTTGTGTACAAGAAATGGATGCAGTTGAGAGTGTTGGTGTTGAGTTTTATGGAGAAAGTGACCTGCCGTTTCATGTTTTAGAGACCCTGGAGTTGCATTATCTGAAAAATTGGAAGAAGTGGTCTCATTGCCAACAAAGTGAGGGTGTTGAAGTTTTCTCTTGCCTGAAAAAGCTTTCCATCAAGGACTGCTCCAAATTGGAGGGTGATTTACCGGAGAAGCTTGATTCATTAGCACAGCTTGAGATTTGCGGATGTGAGGAATTGGTGGTTTCAGTATCCAAATATAAACAGCTTCACCGATCAAACATTGAAAAGTGCAAGATGGTGGTGTACGACACAAGTACAGTTCAGTTTGACTTACTAGAGAGTTTGACATTTTCAAATGTATCAGAATTGAGATTCCAAACAGTGGCGTTCATGAAAAGCTTGAAAAATGTTAAAGAGTTGAGGATCACTGGTTGTGAGGAGCTTACATGTTGCTTTCAAAATGAGGATAGATTACTGCAACACTTGATTTCTCTTGGTCGTCTGTGTATCGAAGACAACTCGATCCTTGTTGAAAAGCTAGGGATTGAAGCAGAGCAGTTGGTGCAATTGCAAATCCTGGATTGCAAGATTGAACGTCTGGCGTTAATCGAGTGTGGAAGGCTTTTGATGGTGCCTGAAGGATATCATCACCTTACATCACTTAAAGAGCTCCGAATAATTAAATGTTCAAGTCTTGTTTCTTTTCCGGATGTTGGTCTGCCACCTTGTCTTCAAGAGCTGCACATAAGTGAATGTTCAAGTCTTGTTTCTTTTCCGGATGTTGGTCTGCCACCTTGTCTCCAAGACATAGTGATTCATGACTGCGAATCTCTGTTGCACTTTGCAAAATACCAGATTCCTCTCAGCCTAAAAAGAATAAAGATTAGGAATTGTGGGAATTTGAAGTCATTAATAGAGAAAGAGGAGGAGGTGGGTAACGGTTCTTCTTCTTCTTGTCTTGAGAGTTTGACGATAGAGAATTGTCCCTCTTTGATGTGCTTATCATGCAAAAGTCAATTACCCAGGTCTCTTAAACAGCTTCGGATAGTGAGTTGTGGACAGCTGGAGCTAATAACTGAGAGGTTCCTCGATGACAGCTGGCAAGTCGAACAAATTTTCATCTTTCACTGCCCAAACCTGAAATCCTTACCCGAGGGACTGTGCCACCTCACCAATCTTCAGTCATTAACTGTTTTGTATTGTCAAAGTCTTATTTCGTTGCCGAGAATGAATGTGTGGCCGAGAATGATACGGATCGTAAATTGCGATAAATTGGAGGTGGCACAGTTGGCGAGAGACATGATGCACACCGAGACATTGGAAATTGATTACTTTGAAGGTTTGACAACTACAACTTCCTTCCCCACCAACCTAACATCACTGGAAATTCACAAGATCAAGAATTGTAAGGCGCTGATGGAATCTCAAGGGTTGCAGAGACTCGCCTCTTTGAGATATTTGTGTCTATGGGGTGAAGATGATGGAGGTCTGGTGTCATTTCCACCAGCTGCTGAGAATTCAAAGGAGACGGAGATACTGCTTCCCAGATCTCTTGTTGTTTTGAGTATTGTAGACTTCCCAAATCTAAAGAAACTGAGCAAGGGCTTTCAATTCCTCACCTCTCTTGAAAGTATTCGGATCAGGAACTGTCCGAAGCTTACAACCCTTCCAGTGGAGGAAGGCCTGCCTCTTTCAGAACTTCGTATTCACAACTGTCCTCTCCTTGAACAGAGATACAAAGGACGATACCGGCACAAGATAGGACACATCCCTGAGGTAGTAATTAATTTCGAAGACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1319

Amino Acids

149.49

Weight (kDa)

6.63

Isoelectric Point (pI)

50.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 86 - 247 3.3e-36 NB-ARC domain
WHD_DRP PF23559 331 - 398 2.6e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 476 - 741 1e-19 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 591 - 714 4.9e-36 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 684
AasI GACNNNNNNGTC 1 cut(s) 650
Acc36I ACCTGC 1 cut(s) 2196
AccB1I GGYRCC 1 cut(s) 2785
AccB7I CCANNNNNTGG 1 cut(s) 2536
AccI GTMKAC 2 cut(s) 855, 3726
AccIII TCCGGA 2 cut(s) 2860, 2929
AciI CCGC 4 cut(s) 10, 1061, 2053, 2376
AclI AACGTT 1 cut(s) 1358
AclWI GGATC 6 cut(s) 1024, 1768, 2585, 2671, 3416, 3800
AcoI YGGCCR 2 cut(s) 367, 3392
AcsI RAATTY 6 cut(s) 1010, 2009, 3052, 3267, 3540, 3670
AcuI CTGAAG 5 cut(s) 26, 777, 1359, 2811, 3317
AcvI CACGTG 1 cut(s) 641
AcyI GRCGYC 1 cut(s) 645
AfaI GTAC 8 cut(s) 310, 789, 1261, 1571, 1672, 2133, 2459, 2470
AfiI CCNNNNNNNGG 9 cut(s) 227, 1382, 1533, 1703, 2099, 2536, 2867, 2936, 3826
AflIII ACRYGT 4 cut(s) 343, 638, 1262, 2600
AjiI CACGTC 2 cut(s) 453, 1097
AjnI CCWGG 6 cut(s) 1121, 1176, 1850, 2211, 2730, 3176
AleI CACNNNNGTG 1 cut(s) 2901
Alw21I GWGCWC 2 cut(s) 56, 2826
AlwI GGATC 6 cut(s) 1024, 1768, 2585, 2671, 3416, 3800
AlwNI CAGNNNCTG 2 cut(s) 3665, 3801
Ama87I CYCGRG 1 cut(s) 3305
Aor13HI TCCGGA 2 cut(s) 2860, 2929
AoxI GGCC 4 cut(s) 367, 591, 3392, 3835
ApeKI GCWGC 9 cut(s) 359, 428, 431, 611, 1202, 1252, 1934, 2893, 3662
ApoI RAATTY 6 cut(s) 1010, 2009, 3052, 3267, 3540, 3670
AseI ATTAAT 2 cut(s) 3065, 3939
Asp700I GAANNNNTTC 3 cut(s) 723, 921, 3786
AspLEI GCGC 1 cut(s) 3568
AspS9I GGNCC 1 cut(s) 218
AsuHPI GGTGA 8 cut(s) 1365, 1975, 2342, 2414, 2795, 3316, 3638, 3763
AsuII TTCGAA 1 cut(s) 3945
AvaI CYCGRG 1 cut(s) 3305
AvaII GGWCC 1 cut(s) 218
AxyI CCTNAGG 1 cut(s) 3927
BalI TGGCCA 1 cut(s) 369
BanI GGYRCC 1 cut(s) 2785
BanII GRGCYC 2 cut(s) 56, 2826
BarI GAAGNNNNNNTAC 2 cut(s) 3082, 3114
BauI CACGAG 1 cut(s) 135
BbrPI CACGTG 1 cut(s) 641
BbsI GAAGAC 4 cut(s) 873, 1899, 2673, 2876
Bbv12I GWGCWC 2 cut(s) 56, 2826
BbvCI CCTCAGC 1 cut(s) 1698
BbvI GCAGC 9 cut(s) 346, 440, 443, 623, 1189, 1264, 1946, 2880, 3649
BceAI ACGGC 2 cut(s) 419, 2176
BciT130I CCWGG 6 cut(s) 1123, 1178, 1852, 2213, 2732, 3178
BciVI GTATCC 1 cut(s) 2413
BfaI CTAG 6 cut(s) 320, 1221, 1640, 1823, 2489, 2693
BfmI CTRYAG 2 cut(s) 587, 1203
BfoI RGCGCY 1 cut(s) 3569
BfuAI ACCTGC 1 cut(s) 2196
BfuI GTATCC 1 cut(s) 2413
BglII AGATCT 1 cut(s) 3701
BisI GCNGC 9 cut(s) 360, 429, 432, 612, 1203, 1253, 1935, 2894, 3663
BlsI GCNGC 9 cut(s) 361, 430, 433, 613, 1204, 1254, 1936, 2895, 3664
Bme1390I CCNGG 6 cut(s) 1123, 1178, 1852, 2213, 2732, 3178
Bme18I GGWCC 1 cut(s) 218
BmeT110I CYCGRG 1 cut(s) 3305
BmgBI CACGTC 2 cut(s) 453, 1097
BmgT120I GGNCC 1 cut(s) 218
BmiI GGNNCC 2 cut(s) 2787, 3239
BmrFI CCNGG 6 cut(s) 1123, 1178, 1852, 2213, 2732, 3178
BmsI GCATC 8 cut(s) 346, 1011, 1513, 1801, 1837, 1921, 2134, 3447
BpiI GAAGAC 4 cut(s) 873, 1899, 2673, 2876
BplI GAGNNNNNCTC 2 cut(s) 470, 502
BpmI CTGGAG 5 cut(s) 333, 444, 515, 2234, 3239
Bpu10I CCTNAGC 1 cut(s) 1698
Bpu14I TTCGAA 1 cut(s) 3945
BpuEI CTTGAG 4 cut(s) 1801, 2387, 3134, 3564
BsaAI YACGTR 1 cut(s) 641
BsaBI GATNNNNATC 2 cut(s) 391, 3407
BsaHI GRCGYC 1 cut(s) 645
BsaI GGTCTC 3 cut(s) 2201, 2253, 3186
BsaJI CCNNGG 5 cut(s) 182, 1272, 2211, 3176, 3306
BsaWI WCCGGW 3 cut(s) 2338, 2860, 2929
BsaXI ACNNNNNCTCC 6 cut(s) 442, 472, 2937, 2967, 3630, 3660
Bsc4I CCNNNNNNNGG 9 cut(s) 227, 1382, 1533, 1703, 2099, 2536, 2867, 2936, 3826
Bse118I RCCGGY 1 cut(s) 3903
Bse1I ACTGG 7 cut(s) 316, 370, 370, 461, 2587, 3540, 3824
Bse21I CCTNAGG 1 cut(s) 3927
Bse3DI GCAATG 4 cut(s) 805, 1727, 1805, 2251
Bse8I GATNNNNATC 2 cut(s) 391, 3407
BseAI TCCGGA 2 cut(s) 2860, 2929
BseBI CCWGG 6 cut(s) 1123, 1178, 1852, 2213, 2732, 3178
BseDI CCNNGG 5 cut(s) 182, 1272, 2211, 3176, 3306
BseGI GGATG 9 cut(s) 241, 392, 1047, 1852, 2149, 2384, 2869, 2938, 3921
BseJI GATNNNNATC 2 cut(s) 391, 3407
BseLI CCNNNNNNNGG 9 cut(s) 227, 1382, 1533, 1703, 2099, 2536, 2867, 2936, 3826
BseMI GCAATG 4 cut(s) 805, 1727, 1805, 2251
BseMII CTCAG 9 cut(s) 105, 929, 1712, 1725, 3031, 3222, 3657, 3740, 3918
BseNI ACTGG 7 cut(s) 316, 370, 370, 461, 2587, 3540, 3824
BseRI GAGGAG 6 cut(s) 1031, 1436, 1686, 2606, 3092, 3095
BseXI GCAGC 9 cut(s) 346, 440, 443, 623, 1189, 1264, 1946, 2880, 3649
BsgI GTGCAG 1 cut(s) 2879
BshFI GGCC 4 cut(s) 369, 593, 3394, 3837
BshNI GGYRCC 1 cut(s) 2785
BsiHKAI GWGCWC 2 cut(s) 56, 2826
BsiHKCI CYCGRG 1 cut(s) 3305
BsiSI CCGG 4 cut(s) 2339, 2861, 2930, 3904
BslFI GGGAC 4 cut(s) 1107, 1816, 3123, 3324
BslI CCNNNNNNNGG 9 cut(s) 227, 1382, 1533, 1703, 2099, 2536, 2867, 2936, 3826
BsmBI CGTCTC 1 cut(s) 3674
BsmFI GGGAC 4 cut(s) 1107, 1816, 3123, 3324
BsnI GGCC 4 cut(s) 369, 593, 3394, 3837
Bso31I GGTCTC 3 cut(s) 2201, 2253, 3186
BsoBI CYCGRG 1 cut(s) 3305
Bsp119I TTCGAA 1 cut(s) 3945
Bsp1286I GDGCHC 2 cut(s) 56, 2826
Bsp13I TCCGGA 2 cut(s) 2860, 2929
Bsp1407I TGTACA 3 cut(s) 787, 1569, 2131
Bsp19I CCATGG 1 cut(s) 182
BspACI CCGC 4 cut(s) 10, 1061, 2053, 2376
BspANI GGCC 4 cut(s) 369, 593, 3394, 3837
BspCNI CTCAG 9 cut(s) 104, 930, 1711, 1726, 3030, 3223, 3658, 3741, 3919
BspEI TCCGGA 2 cut(s) 2860, 2929
BspHI TCATGA 2 cut(s) 2544, 2971
BspLI GGNNCC 2 cut(s) 2787, 3239
BspMAI CTGCAG 2 cut(s) 591, 1207
BspMI ACCTGC 1 cut(s) 2196
BspPI GGATC 6 cut(s) 1024, 1768, 2585, 2671, 3416, 3800
BspT104I TTCGAA 1 cut(s) 3945
BspT107I GGYRCC 1 cut(s) 2785
BspTNI GGTCTC 3 cut(s) 2201, 2253, 3186
BsrDI GCAATG 4 cut(s) 805, 1727, 1805, 2251
BsrFI RCCGGY 1 cut(s) 3903
BsrGI TGTACA 3 cut(s) 787, 1569, 2131
BsrI ACTGG 7 cut(s) 316, 370, 370, 461, 2587, 3540, 3824
BssAI RCCGGY 1 cut(s) 3903
BssECI CCNNGG 5 cut(s) 182, 1272, 2211, 3176, 3306
BssNI GRCGYC 1 cut(s) 645
BssSI CACGAG 1 cut(s) 135
BssT1I CCWWGG 2 cut(s) 182, 1272
Bst2BI CACGAG 1 cut(s) 135
Bst2UI CCWGG 6 cut(s) 1123, 1178, 1852, 2213, 2732, 3178
BstACI GRCGYC 1 cut(s) 645
BstAPI GCANNNNNTGC 1 cut(s) 2716
BstAUI TGTACA 3 cut(s) 787, 1569, 2131
BstBAI YACGTR 1 cut(s) 641
BstBI TTCGAA 1 cut(s) 3945
BstC8I GCNNGC 5 cut(s) 520, 591, 1970, 3254, 3839
BstDSI CCRYGG 1 cut(s) 182
BstEII GGTNACC 1 cut(s) 804
BstF5I GGATG 9 cut(s) 241, 392, 1047, 1852, 2149, 2384, 2869, 2938, 3921
BstH2I RGCGCY 1 cut(s) 3569
BstHHI GCGC 1 cut(s) 3568
BstMWI GCNNNNNNNGC 7 cut(s) 653, 1199, 1699, 1856, 2716, 3159, 3443
BstNI CCWGG 6 cut(s) 1123, 1178, 1852, 2213, 2732, 3178
BstNSI RCATGY 4 cut(s) 347, 1266, 1954, 2604
BstPI GGTNACC 1 cut(s) 804
BstSCI CCNGG 6 cut(s) 1121, 1176, 1850, 2211, 2730, 3176
BstSFI CTRYAG 2 cut(s) 587, 1203
BstV1I GCAGC 9 cut(s) 346, 440, 443, 623, 1189, 1264, 1946, 2880, 3649
BstV2I GAAGAC 4 cut(s) 873, 1899, 2673, 2876
BstX2I RGATCY 1 cut(s) 3701
BstXI CCANNNNNNTGG 1 cut(s) 387
BstYI RGATCY 1 cut(s) 3701
Bsu36I CCTNAGG 1 cut(s) 3927
BsuI GTATCC 1 cut(s) 2413
BsuRI GGCC 4 cut(s) 369, 593, 3394, 3837
BtgI CCRYGG 1 cut(s) 182
BtrI CACGTC 2 cut(s) 453, 1097
BtsCI GGATG 9 cut(s) 241, 392, 1047, 1852, 2149, 2384, 2869, 2938, 3921
BtsI GCAGTG 3 cut(s) 584, 1616, 3280
BtsIMutI CAGTG 8 cut(s) 584, 1498, 1616, 2541, 2580, 3280, 3533, 3831
BveI ACCTGC 1 cut(s) 2196
Cac8I GCNNGC 5 cut(s) 520, 591, 1970, 3254, 3839
CaiI CAGNNNCTG 2 cut(s) 3665, 3801
CciI TCATGA 2 cut(s) 2544, 2971
CfoI GCGC 1 cut(s) 3568
Cfr10I RCCGGY 1 cut(s) 3903
Cfr13I GGNCC 1 cut(s) 218
CseI GACGC 2 cut(s) 653, 1640
CsiI ACCWGGT 1 cut(s) 1176
Csp6I GTAC 8 cut(s) 309, 788, 1260, 1570, 1671, 2132, 2458, 2469
CspCI CAANNNNNGTGG 2 cut(s) 1738, 1773
CviQI GTAC 8 cut(s) 309, 788, 1260, 1570, 1671, 2132, 2458, 2469
DrdI GACNNNNNNGTC 1 cut(s) 650
DseDI GACNNNNNNGTC 1 cut(s) 650
EaeI YGGCCR 2 cut(s) 367, 3392
EciI GGCGGA 1 cut(s) 1050
Ecl136II GAGCTC 2 cut(s) 54, 2824
Eco130I CCWWGG 2 cut(s) 182, 1272
Eco147I AGGCCT 2 cut(s) 593, 3837
Eco24I GRGCYC 2 cut(s) 56, 2826
Eco31I GGTCTC 3 cut(s) 2201, 2253, 3186
Eco32I GATATC 1 cut(s) 2798
Eco47I GGWCC 1 cut(s) 218
Eco53kI GAGCTC 2 cut(s) 54, 2824
Eco57I CTGAAG 5 cut(s) 26, 777, 1359, 2811, 3317
Eco72I CACGTG 1 cut(s) 641
Eco81I CCTNAGG 1 cut(s) 3927
Eco88I CYCGRG 1 cut(s) 3305
Eco91I GGTNACC 1 cut(s) 804
EcoICRI GAGCTC 2 cut(s) 54, 2824
EcoO65I GGTNACC 1 cut(s) 804
EcoRI GAATTC 1 cut(s) 3670
EcoRII CCWGG 6 cut(s) 1121, 1176, 1850, 2211, 2730, 3176
EcoRV GATATC 1 cut(s) 2798
EcoT14I CCWWGG 2 cut(s) 182, 1272
EcoT22I ATGCAT 1 cut(s) 1173
EcoT38I GRGCYC 2 cut(s) 56, 2826
ErhI CCWWGG 2 cut(s) 182, 1272
Esp3I CGTCTC 1 cut(s) 3674
FaqI GGGAC 4 cut(s) 1107, 1816, 3123, 3324
FauI CCCGC 1 cut(s) 2046
FauNDI CATATG 1 cut(s) 1381
FblI GTMKAC 2 cut(s) 855, 3726
Fnu4HI GCNGC 9 cut(s) 360, 429, 432, 612, 1203, 1253, 1935, 2894, 3663
FokI GGATG 9 cut(s) 248, 399, 1054, 1859, 2156, 2391, 2876, 2945, 3908
FriOI GRGCYC 2 cut(s) 56, 2826
Fsp4HI GCNGC 9 cut(s) 360, 429, 432, 612, 1203, 1253, 1935, 2894, 3663
FspBI CTAG 6 cut(s) 320, 1221, 1640, 1823, 2489, 2693
GlaI GCGC 1 cut(s) 3567
GluI GCNGC 9 cut(s) 360, 429, 432, 612, 1203, 1253, 1935, 2894, 3663
GsuI CTGGAG 5 cut(s) 333, 444, 515, 2234, 3239
HaeII RGCGCY 1 cut(s) 3569
HaeIII GGCC 4 cut(s) 369, 593, 3394, 3837
HapII CCGG 4 cut(s) 2339, 2861, 2930, 3904
HgaI GACGC 2 cut(s) 653, 1640
HhaI GCGC 1 cut(s) 3568
Hin1I GRCGYC 1 cut(s) 645
Hin6I GCGC 1 cut(s) 3566
HinP1I GCGC 1 cut(s) 3566
HindIII AAGCTT 4 cut(s) 2297, 2345, 2551, 3809
HpaII CCGG 4 cut(s) 2339, 2861, 2930, 3904
HphI GGTGA 8 cut(s) 1365, 1975, 2342, 2414, 2795, 3316, 3638, 3763
Hpy166II GTNNAC 5 cut(s) 856, 2132, 2458, 3212, 3727
Hpy8I GTNNAC 5 cut(s) 856, 2132, 2458, 3212, 3727
HpyCH4IV ACGT 9 cut(s) 452, 640, 841, 1096, 1195, 1258, 1358, 1510, 2749
HpyF10VI GCNNNNNNNGC 7 cut(s) 653, 1199, 1699, 1856, 2716, 3159, 3443
HpySE526I ACGT 9 cut(s) 452, 640, 841, 1096, 1195, 1258, 1358, 1510, 2749
Hsp92I GRCGYC 1 cut(s) 645
HspAI GCGC 1 cut(s) 3566
Kpn2I TCCGGA 2 cut(s) 2860, 2929
LmnI GCTCC 7 cut(s) 51, 478, 608, 2321, 2593, 2829, 3220
Lsp1109I GCAGC 9 cut(s) 346, 440, 443, 623, 1189, 1264, 1946, 2880, 3649
LweI GCATC 8 cut(s) 346, 1011, 1513, 1801, 1837, 1921, 2134, 3447
MabI ACCWGGT 1 cut(s) 1176
MaeI CTAG 6 cut(s) 320, 1221, 1640, 1823, 2489, 2693
MaeII ACGT 9 cut(s) 452, 640, 841, 1096, 1195, 1258, 1358, 1510, 2749
MfeI CAATTG 4 cut(s) 1025, 1709, 1938, 2720
MflI RGATCY 1 cut(s) 3701
MhlI GDGCHC 2 cut(s) 56, 2826
MlsI TGGCCA 1 cut(s) 369
MluNI TGGCCA 1 cut(s) 369
MlyI GAGTC 8 cut(s) 817, 886, 1163, 1241, 1437, 1547, 1790, 3588
MmeI TCCRAC 1 cut(s) 586
Mox20I TGGCCA 1 cut(s) 369
Mph1103I ATGCAT 1 cut(s) 1173
MroI TCCGGA 2 cut(s) 2860, 2929
MroXI GAANNNNTTC 3 cut(s) 723, 921, 3786
MscI TGGCCA 1 cut(s) 369
MslI CAYNNNNRTG 3 cut(s) 1628, 2045, 2901
Msp20I TGGCCA 1 cut(s) 369
MspA1I CMGCKG 4 cut(s) 614, 3217, 3252, 3662
MspI CCGG 4 cut(s) 2339, 2861, 2930, 3904
MspR9I CCNGG 6 cut(s) 1123, 1178, 1852, 2213, 2732, 3178
MunI CAATTG 4 cut(s) 1025, 1709, 1938, 2720
MvaI CCWGG 6 cut(s) 1123, 1178, 1852, 2213, 2732, 3178
MwoI GCNNNNNNNGC 7 cut(s) 653, 1199, 1699, 1856, 2716, 3159, 3443
NcoI CCATGG 1 cut(s) 182
NdeI CATATG 1 cut(s) 1381
NlaIV GGNNCC 2 cut(s) 2787, 3239
NmeAIII GCCGAG 2 cut(s) 3402, 3420
NmuCI GTSAC 6 cut(s) 448, 631, 641, 1097, 1601, 2183
NsiI ATGCAT 1 cut(s) 1173
NspI RCATGY 4 cut(s) 347, 1266, 1954, 2604
NspV TTCGAA 1 cut(s) 3945
OliI CACNNNNGTG 1 cut(s) 2901
PagI TCATGA 2 cut(s) 2544, 2971
PceI AGGCCT 2 cut(s) 593, 3837
PciI ACATGT 3 cut(s) 343, 1262, 2600
PcsI WCGNNNNNNNCGW 1 cut(s) 3417
PdmI GAANNNNTTC 3 cut(s) 723, 921, 3786
PflMI CCANNNNNTGG 1 cut(s) 2536
PfoI TCCNGGA 1 cut(s) 2730
PkrI GCNGC 9 cut(s) 361, 430, 433, 613, 1204, 1254, 1936, 2895, 3664
PleI GAGTC 8 cut(s) 817, 886, 1162, 1241, 1437, 1547, 1789, 3588
PmaCI CACGTG 1 cut(s) 641
PmlI CACGTG 1 cut(s) 641
PpsI GAGTC 8 cut(s) 817, 886, 1162, 1241, 1437, 1547, 1789, 3588
Ppu21I YACGTR 1 cut(s) 641
PscI ACATGT 3 cut(s) 343, 1262, 2600
PshBI ATTAAT 2 cut(s) 3065, 3939
PsiI TTATAA 1 cut(s) 684
Psp124BI GAGCTC 2 cut(s) 56, 2826
Psp1406I AACGTT 1 cut(s) 1358
Psp6I CCWGG 6 cut(s) 1121, 1176, 1850, 2211, 2730, 3176
PspCI CACGTG 1 cut(s) 641
PspEI GGTNACC 1 cut(s) 804
PspGI CCWGG 6 cut(s) 1121, 1176, 1850, 2211, 2730, 3176
PspN4I GGNNCC 2 cut(s) 2787, 3239
PspPI GGNCC 1 cut(s) 218
PsrI GAACNNNNNNTAC 2 cut(s) 2115, 2147
PstI CTGCAG 2 cut(s) 591, 1207
PstNI CAGNNNCTG 2 cut(s) 3665, 3801
PsuI RGATCY 1 cut(s) 3701
PvuII CAGCTG 4 cut(s) 614, 3217, 3252, 3662
RsaI GTAC 8 cut(s) 310, 789, 1261, 1571, 1672, 2133, 2459, 2470
RsaNI GTAC 8 cut(s) 309, 788, 1260, 1570, 1671, 2132, 2458, 2469
RseI CAYNNNNRTG 3 cut(s) 1628, 2045, 2901
SacI GAGCTC 2 cut(s) 56, 2826
SatI GCNGC 9 cut(s) 360, 429, 432, 612, 1203, 1253, 1935, 2894, 3663
Sau96I GGNCC 1 cut(s) 218
SchI GAGTC 8 cut(s) 817, 886, 1163, 1241, 1437, 1547, 1790, 3588
ScrFI CCNGG 6 cut(s) 1123, 1178, 1852, 2213, 2732, 3178
SduI GDGCHC 2 cut(s) 56, 2826
SexAI ACCWGGT 1 cut(s) 1176
SfaNI GCATC 8 cut(s) 346, 1011, 1513, 1801, 1837, 1921, 2134, 3447
SfcI CTRYAG 2 cut(s) 587, 1203
SfuI TTCGAA 1 cut(s) 3945
SinI GGWCC 1 cut(s) 218
SmiMI CAYNNNNRTG 3 cut(s) 1628, 2045, 2901
SmlI CTYRAG 4 cut(s) 1816, 2366, 3113, 3579
SmoI CTYRAG 4 cut(s) 1816, 2366, 3113, 3579
SseBI AGGCCT 2 cut(s) 593, 3837
SsiI CCGC 4 cut(s) 10, 1061, 2053, 2376
SspMI CTAG 6 cut(s) 320, 1221, 1640, 1823, 2489, 2693
SstI GAGCTC 2 cut(s) 56, 2826
StuI AGGCCT 2 cut(s) 593, 3837
StyD4I CCNGG 6 cut(s) 1121, 1176, 1850, 2211, 2730, 3176
StyI CCWWGG 2 cut(s) 182, 1272
TaiI ACGT 9 cut(s) 455, 643, 844, 1099, 1198, 1261, 1361, 1513, 2752
TatI WGTACW 4 cut(s) 787, 1569, 2131, 2468
TscAI CASTG 8 cut(s) 591, 1498, 1623, 2541, 2587, 3287, 3540, 3831
TseFI GTSAC 6 cut(s) 448, 631, 641, 1097, 1601, 2183
TseI GCWGC 9 cut(s) 359, 428, 431, 611, 1202, 1252, 1934, 2893, 3662
Tsp45I GTSAC 6 cut(s) 448, 631, 641, 1097, 1601, 2183
TspGWI ACGGA 2 cut(s) 3421, 3698
TspRI CASTG 8 cut(s) 591, 1498, 1623, 2541, 2587, 3287, 3540, 3831
Van91I CCANNNNNTGG 1 cut(s) 2536
VpaK11BI GGWCC 1 cut(s) 218
VspI ATTAAT 2 cut(s) 3065, 3939
XapI RAATTY 6 cut(s) 1010, 2009, 3052, 3267, 3540, 3670
XbaI TCTAGA 1 cut(s) 1220
XceI RCATGY 4 cut(s) 347, 1266, 1954, 2604
XmiI GTMKAC 2 cut(s) 855, 3726
XmnI GAANNNNTTC 3 cut(s) 723, 921, 3786
XspI CTAG 6 cut(s) 320, 1221, 1640, 1823, 2489, 2693
Zsp2I ATGCAT 1 cut(s) 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.