RchiOBHm_Chr1g0320581

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000070
Physical Location & Seq
Reverse (-)
8235037 .. 8236917
1881 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55072

Sequence Viewer

Length: 1881 bp
ATGAAACTGGAGCTTGCTCAAGTAAGGTACGAAGCTTCATTCAAAAAGTTAAGTTGTACTTTAATAACTCCGAAATGGAGGGCATTACTCCTCGATTACGGAAATTATATATCCACAAAACAAAAGGAGCTATTGAAGAATGTAAACTCAAACATTGAGACTACTTCCTTACCAGCATTGCAAAGGTCACCGAGTTCTATTGTGTTGAATACACCTGTGGCTGGAAGAGATGGGGAAATAAGAAAGATTGTTGGATCATTGTCAAGAGACTATGAGTCTTCTCGTACCACCAATTTTCATTCAGTTGGCATTGTTGGTATGCCAGGAGTCGGCAAGACAACAGTTGCAGCACATGTCTTCATAGCTGATGCTATGGAACAGTTTTACCCAAAGGTGTGGGTATCTGTGTCTCATAACTTCAATCTTGAAAGAGTGACGAGAGCTATTTGTAGGAAAGTCACATCCCAGCCATGTGATCAGAATGATTTCAGTGAAGTGCAAGATGATTTGAATAGGGCAATAGCAGGTAAAAGATTTTTGATTGTTTTAGATGATGTCTGGAGCACATGTGACTACGAGTCATGGACAACAATGCAGTCCCACTTCCGTGTTGGAGCACCAGGAAGTAAAATACTCGTCACAACACGTGAAGAAAAAGTTGCAAAATTGATTGGAGCCACTGAAGTGTACAATTTGAAGGTTCTATCAGGTGAAGAGTGTCTGAATGTATTTATGCAACATATTAACAATCACAGACCACCAAATTTTGATGCAGTATTTGCCAAGAAAATTGTTGAAAAATGCAATGGACTGCCATTGGCGGCAAAAACTCTTGGTGGTATTTTACGTTGTGAAGAAGTTGACAGATGGAATGAAGTATTGGATGACAAGTTGTGGAGTATATCAAATAACGAGAGTAACATACTCCCAGTTTTGAAGCTGAGTTATCATTATCTTCCTTCAACTTTAAAGAGGTGCTTTGCCTATTGCTCAATATTTCCCAATAACTATGAATTTGGGAAGACGCAATTAATCCTTCTATGGATGGCAGAGGGTATTCTTCAGCAGCCAGAAGGAAATAACCAGATGGAAGATATTGGTGGCAACTATTTTGAGGAGCTGTTATCTAGGTCATTATTTCAAAAGTTGAGCAAAAGTAATTCACTTTATGTAATGCATGACCTTGTTGGTGATTTGGCACGGTGGGCCTCTGGTGATACATATTTCAGATTGGAGGATAAGGTGGATGGCAAATGCTCTCCAAAGACTCGTCATTCTTCTTACATTTCCGGTAAGTTTGATGGGGTCACCAAATTTGAAAAGGCATTCTCTGAAGCAAAGAGTCTACGAACTTTCCTACCACTTTCAATCTCACATGGTCATGAGAATTATCTAACTTGTAAGGTTACTTTTGAGAAGTTGCCAAAGTTAGAGTGTTTACGAGTACTTTCTTTGAATGGCTATCAAATAAGTCTGTTGCCAGAATCAATTGGTCATCTGAAGCATCTACGGTACCTTGATCTATCTCACACACTCATAACGAGTTTGCCTGAATCAACAACCACCCTTTACAACTTGCAGACGTTGATATTAGAAAATTGTTCTCAATTGAAGGCACTACCTGCAAAACTGAAGAATTTGGCAAAATTACGTCATCTGAACAACTCATGTGTGCCTTCATTGGAACGAATGCCTCCTCAGCTAAGTCAACTGACTAATTACCAAACATTATCTAATTTTGTGGTGGGCAAAGGAAATGTGTCAAGGGTAGGAGAGATAGGGCCTCTATCGCTTCGCTGGACATTGTGCCTCAAAAGATTGGAAAATGTGACTAATGTTGAGGATGCAACAAGCACAACCTCAGCTCTCTTCAGAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

626

Amino Acids

70.61

Weight (kDa)

8.77

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 79 - 249 1.8e-31 NB-ARC domain
WHD_DRP PF23559 332 - 400 7.1e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 437 - 579 1.6e-17 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 515, 1630
Acc65I GGTACC 1 cut(s) 1512
AccB1I GGYRCC 1 cut(s) 1512
AccI GTMKAC 1 cut(s) 1345
AciI CCGC 1 cut(s) 821
AclWI GGATC 1 cut(s) 262
AcsI RAATTY 4 cut(s) 763, 1013, 1313, 1636
AcuI CTGAAG 6 cut(s) 702, 1046, 1353, 1520, 1652, 1855
AcvI CACGTG 1 cut(s) 647
AfaI GTAC 6 cut(s) 29, 58, 286, 689, 1446, 1514
AfiI CCNNNNNNNGG 2 cut(s) 221, 329
AflIII ACRYGT 3 cut(s) 352, 566, 644
AhdI GACNNNNNGTC 2 cut(s) 274, 577
AjnI CCWGG 2 cut(s) 322, 619
AjuI GAANNNNNNNTTGG 2 cut(s) 863, 895
AleI CACNNNNGTG 2 cut(s) 606, 683
AluBI AGCT 9 cut(s) 13, 35, 130, 365, 443, 940, 1120, 1702, 1865
AluI AGCT 9 cut(s) 13, 35, 130, 365, 443, 940, 1120, 1702, 1865
Alw21I GWGCWC 2 cut(s) 566, 619
Alw26I GTCTC 3 cut(s) 152, 261, 414
AlwI GGATC 1 cut(s) 262
AoxI GGCC 2 cut(s) 1206, 1781
ApeKI GCWGC 2 cut(s) 347, 1066
ApoI RAATTY 4 cut(s) 763, 1013, 1313, 1636
AseI ATTAAT 1 cut(s) 1031
Asp700I GAANNNNTTC 1 cut(s) 485
Asp718I GGTACC 1 cut(s) 1512
AspS9I GGNCC 2 cut(s) 1206, 1781
AsuHPI GGTGA 5 cut(s) 180, 722, 1202, 1226, 1300
BanI GGYRCC 1 cut(s) 1512
BbrPI CACGTG 1 cut(s) 647
BbsI GAAGAC 3 cut(s) 270, 349, 1028
Bbv12I GWGCWC 2 cut(s) 566, 619
BbvCI CCTCAGC 2 cut(s) 1698, 1861
BbvI GCAGC 2 cut(s) 359, 1078
BccI CCATC 6 cut(s) 224, 861, 1039, 1081, 1241, 1295
BciT130I CCWGG 2 cut(s) 324, 621
BclI TGATCA 1 cut(s) 475
BcoDI GTCTC 3 cut(s) 152, 261, 414
BfaI CTAG 1 cut(s) 1128
BfuAI ACCTGC 2 cut(s) 515, 1630
BisI GCNGC 3 cut(s) 348, 822, 1067
BlsI GCNGC 3 cut(s) 349, 823, 1068
BmcAI AGTACT 1 cut(s) 1446
Bme1390I CCNGG 2 cut(s) 324, 621
BmeRI GACNNNNNGTC 2 cut(s) 274, 577
BmgT120I GGNCC 2 cut(s) 1206, 1781
BmiI GGNNCC 2 cut(s) 676, 1514
BmrFI CCNGG 2 cut(s) 324, 621
BmrI ACTGGG 1 cut(s) 923
BmsI GCATC 4 cut(s) 358, 760, 1513, 1834
BmuI ACTGGG 1 cut(s) 923
BpiI GAAGAC 3 cut(s) 270, 349, 1028
BpmI CTGGAG 2 cut(s) 29, 580
Bpu10I CCTNAGC 2 cut(s) 1698, 1861
BsaAI YACGTR 1 cut(s) 647
BsaWI WCCGGW 1 cut(s) 1289
BsaXI ACNNNNNCTCC 2 cut(s) 553, 583
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 329
Bse1I ACTGG 2 cut(s) 12, 929
Bse3DI GCAATG 2 cut(s) 176, 811
BseBI CCWGG 2 cut(s) 324, 621
BseGI GGATG 5 cut(s) 461, 889, 1050, 1252, 1849
BseLI CCNNNNNNNGG 2 cut(s) 221, 329
BseMI GCAATG 2 cut(s) 176, 811
BseMII CTCAG 3 cut(s) 932, 1712, 1875
BseNI ACTGG 2 cut(s) 12, 929
BseRI GAGGAG 3 cut(s) 80, 1130, 1686
BseXI GCAGC 2 cut(s) 359, 1078
BseYI CCCAGC 1 cut(s) 465
BshFI GGCC 2 cut(s) 1208, 1783
BshNI GGYRCC 1 cut(s) 1512
BsiHKAI GWGCWC 2 cut(s) 566, 619
BsiSI CCGG 1 cut(s) 1290
BslFI GGGAC 1 cut(s) 583
BslI CCNNNNNNNGG 2 cut(s) 221, 329
BsmAI GTCTC 3 cut(s) 152, 261, 414
BsmFI GGGAC 1 cut(s) 583
BsmI GAATGC 2 cut(s) 1325, 1695
BsnI GGCC 2 cut(s) 1208, 1783
Bsp1286I GDGCHC 2 cut(s) 566, 619
Bsp1407I TGTACA 1 cut(s) 687
Bsp143I GATC 3 cut(s) 254, 475, 1519
BspACI CCGC 1 cut(s) 821
BspANI GGCC 2 cut(s) 1208, 1783
BspCNI CTCAG 3 cut(s) 933, 1711, 1874
BspHI TCATGA 1 cut(s) 1381
BspLI GGNNCC 2 cut(s) 676, 1514
BspMI ACCTGC 2 cut(s) 515, 1630
BspPI GGATC 1 cut(s) 262
BspT107I GGYRCC 1 cut(s) 1512
BsrDI GCAATG 2 cut(s) 176, 811
BsrGI TGTACA 1 cut(s) 687
BsrI ACTGG 2 cut(s) 12, 929
BssMI GATC 3 cut(s) 254, 475, 1519
Bst2UI CCWGG 2 cut(s) 324, 621
Bst4CI ACNGT 4 cut(s) 343, 381, 1203, 1512
Bst6I CTCTTC 3 cut(s) 220, 708, 1874
BstAPI GCANNNNNTGC 2 cut(s) 779, 1622
BstAUI TGTACA 1 cut(s) 687
BstBAI YACGTR 1 cut(s) 647
BstC8I GCNNGC 1 cut(s) 15
BstDEI CTNAG 4 cut(s) 941, 1698, 1703, 1861
BstEII GGTNACC 2 cut(s) 186, 1306
BstF5I GGATG 5 cut(s) 461, 889, 1050, 1252, 1849
BstKTI GATC 3 cut(s) 257, 478, 1522
BstMAI GTCTC 3 cut(s) 152, 261, 414
BstMBI GATC 3 cut(s) 254, 475, 1519
BstMWI GCNNNNNNNGC 5 cut(s) 779, 1205, 1622, 1699, 1789
BstNI CCWGG 2 cut(s) 324, 621
BstNSI RCATGY 2 cut(s) 356, 570
BstPI GGTNACC 2 cut(s) 186, 1306
BstSCI CCNGG 2 cut(s) 322, 619
BstV1I GCAGC 2 cut(s) 359, 1078
BstV2I GAAGAC 3 cut(s) 270, 349, 1028
BstXI CCANNNNNNTGG 1 cut(s) 396
BsuRI GGCC 2 cut(s) 1208, 1783
BtsCI GGATG 5 cut(s) 461, 889, 1050, 1252, 1849
BtsIMutI CAGTG 2 cut(s) 496, 678
BveI ACCTGC 2 cut(s) 515, 1630
Cac8I GCNNGC 1 cut(s) 15
CciI TCATGA 1 cut(s) 1381
Cfr13I GGNCC 2 cut(s) 1206, 1781
CseI GACGC 1 cut(s) 1033
Csp6I GTAC 6 cut(s) 28, 57, 285, 688, 1445, 1513
CviAII CATG 8 cut(s) 353, 471, 567, 582, 1178, 1376, 1382, 1668
CviQI GTAC 6 cut(s) 28, 57, 285, 688, 1445, 1513
DdeI CTNAG 4 cut(s) 941, 1698, 1703, 1861
DpnI GATC 3 cut(s) 256, 477, 1521
DpnII GATC 3 cut(s) 254, 475, 1519
DraI TTTAAA 1 cut(s) 969
DriI GACNNNNNGTC 2 cut(s) 274, 577
Eam1104I CTCTTC 3 cut(s) 220, 708, 1874
Eam1105I GACNNNNNGTC 2 cut(s) 274, 577
EarI CTCTTC 3 cut(s) 220, 708, 1874
Eco57I CTGAAG 6 cut(s) 702, 1046, 1353, 1520, 1652, 1855
Eco72I CACGTG 1 cut(s) 647
Eco91I GGTNACC 2 cut(s) 186, 1306
EcoO109I RGGNCCY 1 cut(s) 1781
EcoO65I GGTNACC 2 cut(s) 186, 1306
EcoRII CCWGG 2 cut(s) 322, 619
EcoT22I ATGCAT 1 cut(s) 1179
FaeI CATG 8 cut(s) 356, 474, 570, 585, 1181, 1379, 1385, 1671
FalI AAGNNNNNCTT 4 cut(s) 43, 75, 962, 994
FaqI GGGAC 1 cut(s) 583
FatI CATG 8 cut(s) 352, 470, 566, 581, 1177, 1375, 1381, 1667
FbaI TGATCA 1 cut(s) 475
FblI GTMKAC 1 cut(s) 1345
Fnu4HI GCNGC 3 cut(s) 348, 822, 1067
FokI GGATG 5 cut(s) 448, 896, 1057, 1259, 1856
Fsp4HI GCNGC 3 cut(s) 348, 822, 1067
FspBI CTAG 1 cut(s) 1128
GluI GCNGC 3 cut(s) 348, 822, 1067
GsaI CCCAGC 1 cut(s) 469
GsuI CTGGAG 2 cut(s) 29, 580
HaeIII GGCC 2 cut(s) 1208, 1783
HapII CCGG 1 cut(s) 1290
HgaI GACGC 1 cut(s) 1033
Hin1II CATG 8 cut(s) 356, 474, 570, 585, 1181, 1379, 1385, 1671
HincII GTYRAC 2 cut(s) 862, 1709
HindII GTYRAC 2 cut(s) 862, 1709
HindIII AAGCTT 1 cut(s) 33
HinfI GANTC 7 cut(s) 275, 327, 578, 1267, 1342, 1484, 1553
HpaII CCGG 1 cut(s) 1290
HphI GGTGA 5 cut(s) 180, 722, 1202, 1226, 1300
Hpy166II GTNNAC 6 cut(s) 145, 688, 862, 1346, 1439, 1709
Hpy188I TCNGA 8 cut(s) 72, 480, 723, 1229, 1333, 1500, 1659, 1874
Hpy188III TCNNGA 4 cut(s) 264, 425, 559, 1382
Hpy8I GTNNAC 6 cut(s) 145, 688, 862, 1346, 1439, 1709
HpyAV CCTTC 6 cut(s) 691, 969, 1046, 1067, 1606, 1686
HpyCH4III ACNGT 4 cut(s) 343, 381, 1203, 1512
HpyCH4IV ACGT 4 cut(s) 646, 847, 1583, 1651
HpyF10VI GCNNNNNNNGC 5 cut(s) 779, 1205, 1622, 1699, 1789
HpyF3I CTNAG 4 cut(s) 941, 1698, 1703, 1861
HpySE526I ACGT 4 cut(s) 646, 847, 1583, 1651
Hsp92II CATG 8 cut(s) 356, 474, 570, 585, 1181, 1379, 1385, 1671
KpnI GGTACC 1 cut(s) 1516
Ksp22I TGATCA 1 cut(s) 475
Kzo9I GATC 3 cut(s) 254, 475, 1519
LmnI GCTCC 6 cut(s) 10, 127, 561, 614, 674, 1117
Lsp1109I GCAGC 2 cut(s) 359, 1078
LweI GCATC 4 cut(s) 358, 760, 1513, 1834
MaeI CTAG 1 cut(s) 1128
MaeII ACGT 4 cut(s) 646, 847, 1583, 1651
MaeIII GTNAC 9 cut(s) 186, 433, 457, 569, 637, 917, 1306, 1405, 1828
MalI GATC 3 cut(s) 256, 477, 1521
MboI GATC 3 cut(s) 254, 475, 1519
MfeI CAATTG 2 cut(s) 1488, 1607
MhlI GDGCHC 2 cut(s) 566, 619
MlyI GAGTC 5 cut(s) 284, 336, 587, 1261, 1351
MmeI TCCRAC 2 cut(s) 232, 592
Mph1103I ATGCAT 1 cut(s) 1179
MroXI GAANNNNTTC 1 cut(s) 485
MseI TTAA 6 cut(s) 50, 62, 744, 968, 1031, 1879
MslI CAYNNNNRTG 3 cut(s) 606, 683, 1380
MspI CCGG 1 cut(s) 1290
MspR9I CCNGG 2 cut(s) 324, 621
MunI CAATTG 2 cut(s) 1488, 1607
Mva1269I GAATGC 2 cut(s) 1325, 1695
MvaI CCWGG 2 cut(s) 324, 621
MwoI GCNNNNNNNGC 5 cut(s) 779, 1205, 1622, 1699, 1789
NdeII GATC 3 cut(s) 254, 475, 1519
NlaIII CATG 8 cut(s) 356, 474, 570, 585, 1181, 1379, 1385, 1671
NlaIV GGNNCC 2 cut(s) 676, 1514
NmuCI GTSAC 7 cut(s) 186, 433, 457, 569, 637, 1306, 1828
NsiI ATGCAT 1 cut(s) 1179
NspI RCATGY 2 cut(s) 356, 570
OliI CACNNNNGTG 2 cut(s) 606, 683
PagI TCATGA 1 cut(s) 1381
PciI ACATGT 2 cut(s) 352, 566
PctI GAATGC 2 cut(s) 1325, 1695
PdmI GAANNNNTTC 1 cut(s) 485
PfeI GAWTC 2 cut(s) 1484, 1553
PkrI GCNGC 3 cut(s) 349, 823, 1068
PleI GAGTC 5 cut(s) 283, 335, 586, 1261, 1350
PmaCI CACGTG 1 cut(s) 647
PmlI CACGTG 1 cut(s) 647
PpsI GAGTC 5 cut(s) 283, 335, 586, 1261, 1350
Ppu21I YACGTR 1 cut(s) 647
PscI ACATGT 2 cut(s) 352, 566
PshBI ATTAAT 1 cut(s) 1031
Psp6I CCWGG 2 cut(s) 322, 619
PspCI CACGTG 1 cut(s) 647
PspEI GGTNACC 2 cut(s) 186, 1306
PspFI CCCAGC 1 cut(s) 465
PspGI CCWGG 2 cut(s) 322, 619
PspN4I GGNNCC 2 cut(s) 676, 1514
PspPI GGNCC 2 cut(s) 1206, 1781
RsaI GTAC 6 cut(s) 29, 58, 286, 689, 1446, 1514
RsaNI GTAC 6 cut(s) 28, 57, 285, 688, 1445, 1513
RseI CAYNNNNRTG 3 cut(s) 606, 683, 1380
SaqAI TTAA 6 cut(s) 50, 62, 744, 968, 1031, 1879
SatI GCNGC 3 cut(s) 348, 822, 1067
Sau3AI GATC 3 cut(s) 254, 475, 1519
Sau96I GGNCC 2 cut(s) 1206, 1781
ScaI AGTACT 1 cut(s) 1446
SchI GAGTC 5 cut(s) 284, 336, 587, 1261, 1351
ScrFI CCNGG 2 cut(s) 324, 621
SduI GDGCHC 2 cut(s) 566, 619
SfaNI GCATC 4 cut(s) 358, 760, 1513, 1834
SmiMI CAYNNNNRTG 3 cut(s) 606, 683, 1380
SmlI CTYRAG 1 cut(s) 18
SmoI CTYRAG 1 cut(s) 18
SsiI CCGC 1 cut(s) 821
SspI AATATT 1 cut(s) 996
SspMI CTAG 1 cut(s) 1128
StyD4I CCNGG 2 cut(s) 322, 619
TaaI ACNGT 4 cut(s) 343, 381, 1203, 1512
TaiI ACGT 4 cut(s) 649, 850, 1586, 1654
TaqI TCGA 1 cut(s) 93
TatI WGTACW 3 cut(s) 56, 687, 1444
TauI GCSGC 1 cut(s) 824
TfiI GAWTC 2 cut(s) 1484, 1553
Tru1I TTAA 6 cut(s) 50, 62, 744, 968, 1031, 1879
Tru9I TTAA 6 cut(s) 50, 62, 744, 968, 1031, 1879
TscAI CASTG 2 cut(s) 496, 685
TseFI GTSAC 7 cut(s) 186, 433, 457, 569, 637, 1306, 1828
TseI GCWGC 2 cut(s) 347, 1066
Tsp45I GTSAC 7 cut(s) 186, 433, 457, 569, 637, 1306, 1828
TspDTI ATGAA 7 cut(s) 17, 27, 287, 349, 888, 1026, 1668
TspGWI ACGGA 2 cut(s) 114, 596
TspRI CASTG 2 cut(s) 496, 685
VspI ATTAAT 1 cut(s) 1031
XapI RAATTY 4 cut(s) 763, 1013, 1313, 1636
XceI RCATGY 2 cut(s) 356, 570
XcmI CCANNNNNNNNNTGG 1 cut(s) 608
XmiI GTMKAC 1 cut(s) 1345
XmnI GAANNNNTTC 1 cut(s) 485
XspI CTAG 1 cut(s) 1128
ZrmI AGTACT 1 cut(s) 1446
Zsp2I ATGCAT 1 cut(s) 1179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.