MD11G1033200.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000070
Physical Location & Seq
Reverse (-)
2868576 .. 2875004
6429 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1033200.v1.1.491

Sequence Viewer

Length: 4251 bp
ATGGCACTGGGAGAGGTTTTTCTTGCGGCGTTTCTGCAGCTGCTGCTCGACAGGTTGACCCCTCGCGAGATTCTCGACTACTTTGGAAACTTCCGGGGCGTCGGAAAGAAGCTAGAGAAGTGGAGGTCCACGTTGTCTACAATCGGAGCGGTGCTGAGCGACGCCGAGGAAAGGCAACTGACTGAAGGTGGTGTAAAATTGTGGCTTGATGATCTAAGAGACTTGGCCTATGCTGTCGAAGACATGTTGGACAAATTTGCCGCTCAAATGTTGAAGCGCATGATAGAGGGATGTGATCAAGCCAGCACAAGCAACAAGGTACGGAGATCATTTTATAAAGCTCAATTGAGTTTTGATATGAACTCCGAAATGAAGAAGATTACGGAGCGGTTGCAAGACATATCTGAACGGAAAGATAAGTTTGGCTTGAAAGATACTGGGGCGTCGGCTATGGAATCGCGAAGTCTACAAAGTTCAGGCGTGTTGGATGAAAAGCTTGTTGTTGGAAGAGATGGTGACAAAGGGGAGATTATTGAATTGTTGTCAAGAAAATACGAACAGGTAGGTGCTCTCAATTTTGGGGTAGTTGCTATTGTTGGCATGCCCGGAGTCGGGAAGACGACACTTGCTCAACTTGTATTCAACCACAAAGATGATGCCATGAAGGAGTTTGAGCCAAAGGTGTGGGTATCCGTGTCTGATGACTTCAATGTTGTGCGAGTGACAAAGGCAATTCTTGAGTCAATCACATCTGGACCCGTTAAAGTGGAGGAGTTTAGTAAAATGCAGCATGATTTGAGTGAGCAATTAAGAGGAAAAAAGTTTTTAATCGTGTTAGATGATATTTGGAACAAGGGTGACTATGATCTATACGATCTCTGGACAAGACTTCAATCCCCTTTTGGTGTCGGCGCAGGAGGAAGTAAGATAATTGTGACAACACGTGATGTGAATGTTGCAAAGATTATGGGAGCTGCTGGAGTTCATAATTTGGAGTGTATGGCAGATGATGATTGTTTTAAAATATTTGAGCGACATGCATTCGGGGAAATTAATGATGGAAAACCAGTAAATTCTGAGTTAATTCGAACAAAAATTGTTGAAAAATGTCGTGGGTTACCATTTGCTGCAAGGACTCTCGGTGGTCTTTTACGTTGCAAAGAAAAGGATGAGTGGGGAGAAATATTGAACAACAAATTATGGAATATAGCAGATAAGAGTGACATTCTCCCGGTACTAAAGTTGAGCTATCACTGTCTTCCATCAAATTTGAAGAGGTGTTTTGCCTATTGCTCAATACTTCCAAATGACTATGAATTTGGGGAGAAGCAGCTCGTCCTTTTGTGGATGGCAGAGGGTTTGATTCAACAAAAACCTGAACACAATAAACAAATGGAGGATTTGGGCCGCGACTACTTTCGAGAGCTATTATCGAGGTCGTTGTTTCAAAAATCCAGCAAAAACAATTCACGATATGTAATGCATGACCTCGTTAATGATTTAGCACAATGGGCAGCAGGAGAAATATGTTTTAGGTTGGACGATAAGCAAGGTGATAACTTGCAACCCACCTGCTTTCGAAGGGCTCGCCATTCGTCTTTCATTGCTGGTCAATTTGATGGAGTTATGAGATTTGAGGACTTTCCTAAAGTTGAACGTTTGCGAACATTCCTGCCACTTTCGCTTTCAGATTCCAGGGGTTGGAGTAAATATTTGTCTCATAAGGTTCTTTTTGAGTTATTACCACAGTTGCAGTACTTACGTGTGCTCTCTTTGAATGGCTATGCAATAACTGAGCTGCCAGAGTCAATCAGTGATTTGAGGTTGTTACATTATCTTGACCTTTCCTACACAGATATAATCAGTTTGCCTAAATCAACAAGCACTCTTTACAACCTGCAAACATTGATATTGGAAGGTTGTTCTAAATTGAAGTCATTGCCTGCGAACATGAGTAATCTAATTAATTTGCGCCATCTCAACAACTCAGTTGCATATTCATTGGAAGGAATGCCTCCACAACTAGGTAAATTGGTGAATCTCCAATCATTGTCTAATTTTGTGGTTAGTGATGATAGTGGTCAATCAAGGATAAGAGAGATAGAGTTCCTATTGCATCTCTGCGGGACATTGTGCATCTCAAGATTGGAGAATGTGATTGAAGTCGAGGATGCTCGGAGGACCAACCTAAAATGCAAGGAAGGGATTGATTCGTTGGTCCTAGAATGGTCTCATTCAAGCGACACAAGAGAAATGGAATCCGCTGTGCTTGACATGTTACAGCCTCATACAAAGCTCAAGGAGCTCACCATCAAGAGCTATGACGGAAATGAATTTTCATCATGGGTTGGAGTTCCTTTGTTCTCTAATATGGTGCTTGTGCGCTTAGAGGAATGCAACAATTGTTTATTGTTGCCACCTCTTGGACAATTGCCTCATCTCAAAGAACTTTATATTAGGGGAATGAATGCAGTGGAAAGTGTTGGTGCTGAGTTTTATGGAGAGTGTATCTTGCCTTTTCCGCTATTAGAGACTCTTGAGTTTGTGGATATGCAGCATTGGAAGGAATGGCTTCCTTTCCAACTGGATCACATAAGTGGTACTTTCCCTTGCCTGAAAACACTCTTCGTAAAAAAATGTTCTAAACTGGGGGGTAAGCTGCCAGAGAACCTCAATTCTTTAACAAAGCTTGAAATTGTTAAATGTGAAGAATTAGTGGTTTCGATTGCCAACTACAAACAACTTCGTCAGTTAAACATTGATGGTTGTAAAGTGTTTGTGCATACAGCTGCTAAGGTTGAGTTTGAGTTGTTAGAGTCCTTATGCCTTTCAAACATTTCGGAACTGCTGTCTCTGCAAACAGGGGAATTGTTCAAGAAGGGATTAACCAAGGTTAGAGATTTGAAGATTAATGGATGTGAGGAGCTGATGTCTTCATTGAAGAATGAGGGTAGATTATTGCAGCAGTTGACTTCTCTTGGCCGTTTGGAAATTGAAGACAACTCTCCTCTAGTTGAAGAATTGGGCAAAGAAGCAGAAGAGTTGTTGCAATTGCAAATATTGGAGTGCAAGCTTGAATGTTTGGAGCTAAATAAGTGCGAAAATCTTTTAAAGCTACCAGAAGGGTTAAATCAGTTGTCATCTCTTCAAGAGCTTTGCATACACAGATGTTCACTTCTAGTTTCTTTTCCAGACATTGGTCTGCCACCTTCTCTTAAAAACATCAAGATTACAGAGTGCCATTCATTGATATATTTTGCAAAATTCCAGATTCCCCAAAATCTCAGAAGAATAGAGATAAGAGATTGCAAAAGTTTGAAATCACTAGTAGATGAGGAGGTTATTGGTTCTTGGCCGTCGTCTTTTTCTCACAATTGTCTTGAGTACTTGAAAATCCAGGAATGTCAATCTCTGACGTCGTTGTCATTGAGTGGCCAGCTTCTCAGGACACTTAAACACCTTGAGATGTACGATTGTTCACAGCTGGAGTTAATAGCACAGGACGAGTTCTTCTGCGACAATACTAATCACTGTCTTGAATATGTTAGGATCTGGAAGTGCCAAAATCTGAAATCCTTACCGAATGGCTTACGCCACCTCATCAATCTTCAAACGTTGGATATTTATCACTGTGAAAGTCTTGTTTCCATCCCCAGACTGAGTAAGGGGAGAATAGCCTCCAAGCTGAGAGAGGTCGAGATCACAGATTGCGACAAATTGAAGGTGTTGCCAGAATACATGCAGAATCTCAACTCTCTTGAGGAATTGATGATTGACTACCGTGAAGGTTTGAGTTGCTCCTTTCCCCCCAACCTCACATCACTTACAATTTGGAAGGTCAAGAGCTGTAAGTCATTTTGGGAGTTGGAGTGGGGATTGCACAGACTCACCTCTCTTAGAAAGTTGTGGATCTACGGCAATGATCCGGATACGGTGTCGTTTCCACCCGACATGGCCCAGATGGAGACGCTCCTCCCCAAATCTCTCACTGATCTCCGAATACATGGCTTTCTGAATCTGAAGAAACTGAGCAGCAAGGGCTTTCAATTCCTCACCTCCCTTCAATCTCTGAGCCTTTTCAAATGTCCAAAGTTAGCATCCATTCCAGAGGAGTGTCTACCTCTTTCGCTTAGGCAACTTTGGATCTATGGGTGTCCTATGCTAGAAGAGAGATGCCAACGAGGAAAAGGACGCTACTGGCACAAAATATCCCACATCCCTTACATAGACATAGTTGGGAAGAGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1417

Amino Acids

161.5

Weight (kDa)

6.12

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 11 - 102 5.9e-20 Rx N-terminal domain
NB-ARC PF00931 175 - 348 2.6e-36 NB-ARC domain
WHD_DRP PF23559 433 - 502 2.6e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 579 - 849 3.6e-17 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 698 - 822 1.7e-35 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 336
AarI CACCTGC 1 cut(s) 1580
AasI GACNNNNNNGTC 1 cut(s) 3421
AatII GACGTC 1 cut(s) 3419
Acc36I ACCTGC 2 cut(s) 1580, 1905
AccB7I CCANNNNNTGG 3 cut(s) 1701, 2423, 3197
AccBSI CCGCTC 3 cut(s) 149, 263, 388
AccI GTMKAC 3 cut(s) 137, 466, 4117
AccII CGCG 3 cut(s) 66, 460, 1410
AccIII TCCGGA 1 cut(s) 3925
AciI CCGC 8 cut(s) 26, 149, 261, 388, 1408, 2124, 2262, 2522
AclI AACGTT 2 cut(s) 1657, 3614
AclWI GGATC 5 cut(s) 2595, 3557, 3917, 3917, 4151
AcoI YGGCCR 3 cut(s) 2980, 3353, 3433
AcsI RAATTY 6 cut(s) 254, 1072, 1267, 1316, 2333, 3263
AcuI CTGAAG 2 cut(s) 204, 4040
AcvI CACGTG 1 cut(s) 944
AcyI GRCGYC 4 cut(s) 99, 162, 443, 3416
AfaI GTAC 6 cut(s) 321, 1236, 1757, 2602, 3386, 3470
AfiI CCNNNNNNNGG 7 cut(s) 171, 611, 612, 1701, 2024, 2423, 3197
AflIII ACRYGT 4 cut(s) 243, 941, 1762, 2274
AhlI ACTAGT 1 cut(s) 3325
AjnI CCWGG 2 cut(s) 1694, 3396
AleI CACNNNNGTG 1 cut(s) 2595
Alw21I GWGCWC 3 cut(s) 571, 1770, 2307
Alw26I GTCTC 6 cut(s) 213, 1722, 2235, 2525, 2856, 3959
AlwI GGATC 5 cut(s) 2595, 3557, 3917, 3917, 4151
AlwNI CAGNNNCTG 1 cut(s) 43
Aor13HI TCCGGA 1 cut(s) 3925
AoxI GGCC 6 cut(s) 225, 1405, 2980, 3353, 3433, 3954
ApoI RAATTY 6 cut(s) 254, 1072, 1267, 1316, 2333, 3263
ArsI GACNNNNNNTTYG 2 cut(s) 1301, 1333
AseI ATTAAT 3 cut(s) 1053, 1965, 2910
Asp700I GAANNNNTTC 1 cut(s) 2571
AspLEI GCGC 4 cut(s) 279, 914, 1974, 2385
AspS9I GGNCC 6 cut(s) 126, 755, 1405, 2181, 2218, 3955
AsuC2I CCSGG 3 cut(s) 95, 606, 1232
AsuHPI GGTGA 7 cut(s) 527, 869, 1565, 2047, 2299, 3880, 4045
AsuII TTCGAA 2 cut(s) 1087, 1579
AvaII GGWCC 4 cut(s) 126, 755, 2181, 2218
BaeI ACNNNNGTAYC 2 cut(s) 1226, 1259
BalI TGGCCA 1 cut(s) 3435
BanII GRGCYC 2 cut(s) 1588, 2307
BbrPI CACGTG 1 cut(s) 944
BbsI GAAGAC 5 cut(s) 246, 623, 1250, 2925, 3003
Bbv12I GWGCWC 3 cut(s) 571, 1770, 2307
BceAI ACGGC 3 cut(s) 2967, 3340, 3931
BciT130I CCWGG 2 cut(s) 1696, 3398
BciVI GTATCC 2 cut(s) 700, 3922
BclI TGATCA 1 cut(s) 295
BcnI CCSGG 3 cut(s) 95, 606, 1232
BcoDI GTCTC 6 cut(s) 213, 1722, 2235, 2525, 2856, 3959
BcuI ACTAGT 1 cut(s) 3325
BfaI CTAG 7 cut(s) 113, 2024, 2222, 3011, 3179, 3326, 4163
BfmI CTRYAG 1 cut(s) 35
BfuAI ACCTGC 2 cut(s) 1580, 1905
BfuI GTATCC 2 cut(s) 700, 3922
BlpI GCTNAGC 1 cut(s) 155
BmcAI AGTACT 2 cut(s) 1757, 3386
Bme1390I CCNGG 5 cut(s) 95, 606, 1232, 1696, 3398
Bme18I GGWCC 4 cut(s) 126, 755, 2181, 2218
BmgT120I GGNCC 6 cut(s) 126, 755, 1405, 2181, 2218, 3955
BmiI GGNNCC 1 cut(s) 757
BmrFI CCNGG 5 cut(s) 95, 606, 1232, 1696, 3398
BmrI ACTGGG 3 cut(s) 17, 447, 2657
BmsI GCATC 6 cut(s) 646, 2125, 2145, 2161, 4106, 4163
BmuI ACTGGG 3 cut(s) 17, 447, 2657
BoxI GACNNNNGTC 1 cut(s) 3198
BpiI GAAGAC 5 cut(s) 246, 623, 1250, 2925, 3003
BpmI CTGGAG 2 cut(s) 999, 3506
Bpu10I CCTNAGC 2 cut(s) 2793, 4130
Bpu1102I GCTNAGC 1 cut(s) 155
Bpu14I TTCGAA 2 cut(s) 1087, 1579
BpuEI CTTGAG 7 cut(s) 758, 2125, 2282, 2558, 3401, 3482, 3779
BpuMI CCSGG 3 cut(s) 95, 606, 1232
BsaAI YACGTR 2 cut(s) 944, 1763
BsaBI GATNNNNATC 1 cut(s) 3624
BsaHI GRCGYC 4 cut(s) 99, 162, 443, 3416
BsaI GGTCTC 1 cut(s) 2235
BsaJI CCNNGG 4 cut(s) 94, 165, 1695, 2889
BsaWI WCCGGW 1 cut(s) 3925
BsaXI ACNNNNNCTCC 4 cut(s) 2915, 2945, 3854, 3884
Bsc4I CCNNNNNNNGG 7 cut(s) 171, 611, 612, 1701, 2024, 2423, 3197
Bse1I ACTGG 6 cut(s) 12, 442, 1067, 2589, 2652, 4202
Bse3DI GCAATG 3 cut(s) 1602, 1937, 3925
Bse8I GATNNNNATC 1 cut(s) 3624
BseAI TCCGGA 1 cut(s) 3925
BseBI CCWGG 2 cut(s) 1696, 3398
BseDI CCNNGG 4 cut(s) 94, 165, 1695, 2889
BseGI GGATG 9 cut(s) 296, 493, 1174, 1353, 2176, 2921, 3648, 4097, 4215
BseJI GATNNNNATC 1 cut(s) 3624
BseLI CCNNNNNNNGG 7 cut(s) 171, 611, 612, 1701, 2024, 2423, 3197
BseMI GCAATG 3 cut(s) 1602, 1937, 3925
BseNI ACTGG 6 cut(s) 12, 442, 1067, 2589, 2652, 4202
BseRI GAGGAG 6 cut(s) 785, 2936, 2997, 3350, 3962, 4124
Bsh1236I CGCG 3 cut(s) 66, 460, 1410
BshFI GGCC 6 cut(s) 227, 1407, 2982, 3355, 3435, 3956
BsiHKAI GWGCWC 3 cut(s) 571, 1770, 2307
BsiSI CCGG 4 cut(s) 94, 606, 1232, 3926
BslFI GGGAC 1 cut(s) 2140
BslI CCNNNNNNNGG 7 cut(s) 171, 611, 612, 1701, 2024, 2423, 3197
BsmAI GTCTC 6 cut(s) 213, 1722, 2235, 2525, 2856, 3959
BsmBI CGTCTC 1 cut(s) 3959
BsmFI GGGAC 1 cut(s) 2140
BsmI GAATGC 4 cut(s) 1040, 2016, 2399, 2473
BsnI GGCC 6 cut(s) 227, 1407, 2982, 3355, 3435, 3956
Bso31I GGTCTC 1 cut(s) 2235
Bsp119I TTCGAA 2 cut(s) 1087, 1579
Bsp1286I GDGCHC 4 cut(s) 571, 1588, 1770, 2307
Bsp13I TCCGGA 1 cut(s) 3925
Bsp1720I GCTNAGC 1 cut(s) 155
Bsp68I TCGCGA 2 cut(s) 66, 460
BspACI CCGC 8 cut(s) 26, 149, 261, 388, 1408, 2124, 2262, 2522
BspANI GGCC 6 cut(s) 227, 1407, 2982, 3355, 3435, 3956
BspEI TCCGGA 1 cut(s) 3925
BspFNI CGCG 3 cut(s) 66, 460, 1410
BspLI GGNNCC 1 cut(s) 757
BspMAI CTGCAG 1 cut(s) 39
BspMI ACCTGC 2 cut(s) 1580, 1905
BspPI GGATC 5 cut(s) 2595, 3557, 3917, 3917, 4151
BspT104I TTCGAA 2 cut(s) 1087, 1579
BspTNI GGTCTC 1 cut(s) 2235
BsrBI CCGCTC 3 cut(s) 149, 263, 388
BsrDI GCAATG 3 cut(s) 1602, 1937, 3925
BsrI ACTGG 6 cut(s) 12, 442, 1067, 2589, 2652, 4202
BssECI CCNNGG 4 cut(s) 94, 165, 1695, 2889
BssNI GRCGYC 4 cut(s) 99, 162, 443, 3416
BssT1I CCWWGG 1 cut(s) 2889
Bst2UI CCWGG 2 cut(s) 1696, 3398
Bst4CI ACNGT 6 cut(s) 1256, 1749, 3533, 3632, 3782, 3934
Bst6I CTCTTC 7 cut(s) 502, 1268, 2630, 3033, 3150, 4161, 4235
BstACI GRCGYC 4 cut(s) 99, 162, 443, 3416
BstAPI GCANNNNNTGC 1 cut(s) 43
BstBAI YACGTR 2 cut(s) 944, 1763
BstBI TTCGAA 2 cut(s) 1087, 1579
BstC8I GCNNGC 6 cut(s) 304, 602, 1588, 1944, 3071, 3437
BstEII GGTNACC 1 cut(s) 1116
BstF5I GGATG 9 cut(s) 296, 493, 1174, 1353, 2176, 2921, 3648, 4097, 4215
BstFNI CGCG 3 cut(s) 66, 460, 1410
BstHHI GCGC 4 cut(s) 279, 914, 1974, 2385
BstMAI GTCTC 6 cut(s) 213, 1722, 2235, 2525, 2856, 3959
BstMWI GCNNNNNNNGC 7 cut(s) 43, 1511, 1681, 2302, 2521, 2854, 4038
BstNI CCWGG 2 cut(s) 1696, 3398
BstNSI RCATGY 5 cut(s) 247, 604, 1040, 2278, 3742
BstPAI GACNNNNGTC 1 cut(s) 3198
BstPI GGTNACC 1 cut(s) 1116
BstSCI CCNGG 5 cut(s) 93, 604, 1230, 1694, 3396
BstSFI CTRYAG 1 cut(s) 35
BstUI CGCG 3 cut(s) 66, 460, 1410
BstV2I GAAGAC 5 cut(s) 246, 623, 1250, 2925, 3003
BstX2I RGATCY 3 cut(s) 3549, 3909, 4143
BstXI CCANNNNNNTGG 1 cut(s) 684
BstYI RGATCY 3 cut(s) 3549, 3909, 4143
BsuI GTATCC 2 cut(s) 700, 3922
BsuRI GGCC 6 cut(s) 227, 1407, 2982, 3355, 3435, 3956
BtsCI GGATG 9 cut(s) 296, 493, 1174, 1353, 2176, 2921, 3648, 4097, 4215
BtsI GCAGTG 1 cut(s) 2478
BtsIMutI CAGTG 7 cut(s) 5, 1252, 1819, 2478, 3529, 3628, 3987
BtuMI TCGCGA 2 cut(s) 66, 460
BveI ACCTGC 2 cut(s) 1580, 1905
Cac8I GCNNGC 6 cut(s) 304, 602, 1588, 1944, 3071, 3437
CaiI CAGNNNCTG 1 cut(s) 43
CfoI GCGC 4 cut(s) 279, 914, 1974, 2385
Cfr13I GGNCC 6 cut(s) 126, 755, 1405, 2181, 2218, 3955
CseI GACGC 5 cut(s) 88, 170, 432, 3976, 4200
Csp6I GTAC 6 cut(s) 320, 1235, 1756, 2601, 3385, 3469
CviQI GTAC 6 cut(s) 320, 1235, 1756, 2601, 3385, 3469
DraI TTTAAA 2 cut(s) 1021, 3111
DrdI GACNNNNNNGTC 1 cut(s) 3421
DseDI GACNNNNNNGTC 1 cut(s) 3421
EaeI YGGCCR 3 cut(s) 2980, 3353, 3433
Eam1104I CTCTTC 7 cut(s) 502, 1268, 2630, 3033, 3150, 4161, 4235
EarI CTCTTC 7 cut(s) 502, 1268, 2630, 3033, 3150, 4161, 4235
Ecl136II GAGCTC 1 cut(s) 2305
Eco130I CCWWGG 1 cut(s) 2889
Eco24I GRGCYC 2 cut(s) 1588, 2307
Eco31I GGTCTC 1 cut(s) 2235
Eco47I GGWCC 4 cut(s) 126, 755, 2181, 2218
Eco53kI GAGCTC 1 cut(s) 2305
Eco57I CTGAAG 2 cut(s) 204, 4040
Eco72I CACGTG 1 cut(s) 944
Eco91I GGTNACC 1 cut(s) 1116
EcoICRI GAGCTC 1 cut(s) 2305
EcoO65I GGTNACC 1 cut(s) 1116
EcoRII CCWGG 2 cut(s) 1694, 3396
EcoT14I CCWWGG 1 cut(s) 2889
EcoT22I ATGCAT 2 cut(s) 1042, 1485
EcoT38I GRGCYC 2 cut(s) 1588, 2307
ErhI CCWWGG 1 cut(s) 2889
Esp3I CGTCTC 1 cut(s) 3959
FalI AAGNNNNNCTT 4 cut(s) 410, 442, 2587, 2619
FaqI GGGAC 1 cut(s) 2140
FauI CCCGC 1 cut(s) 2117
FbaI TGATCA 1 cut(s) 295
FblI GTMKAC 3 cut(s) 137, 466, 4117
FokI GGATG 9 cut(s) 303, 500, 1181, 1360, 2183, 2928, 3635, 4084, 4202
FriOI GRGCYC 2 cut(s) 1588, 2307
FspBI CTAG 7 cut(s) 113, 2024, 2222, 3011, 3179, 3326, 4163
GlaI GCGC 4 cut(s) 278, 913, 1973, 2384
GsuI CTGGAG 2 cut(s) 999, 3506
HaeIII GGCC 6 cut(s) 227, 1407, 2982, 3355, 3435, 3956
HapII CCGG 4 cut(s) 94, 606, 1232, 3926
HgaI GACGC 5 cut(s) 88, 170, 432, 3976, 4200
HhaI GCGC 4 cut(s) 279, 914, 1974, 2385
Hin1I GRCGYC 4 cut(s) 99, 162, 443, 3416
Hin6I GCGC 4 cut(s) 277, 912, 1972, 2383
HinP1I GCGC 4 cut(s) 277, 912, 1972, 2383
HincII GTYRAC 2 cut(s) 57, 2970
HindII GTYRAC 2 cut(s) 57, 2970
HindIII AAGCTT 3 cut(s) 494, 2687, 3071
HpaII CCGG 4 cut(s) 94, 606, 1232, 3926
HphI GGTGA 7 cut(s) 527, 869, 1565, 2047, 2299, 3880, 4045
Hpy166II GTNNAC 8 cut(s) 57, 129, 138, 467, 2970, 3173, 3479, 4118
Hpy8I GTNNAC 8 cut(s) 57, 129, 138, 467, 2970, 3173, 3479, 4118
Hpy99I CGWCG 5 cut(s) 104, 164, 448, 3361, 3421
HpyCH4III ACNGT 6 cut(s) 1256, 1749, 3533, 3632, 3782, 3934
HpyCH4IV ACGT 7 cut(s) 131, 943, 1153, 1657, 1762, 3416, 3614
HpyF10VI GCNNNNNNNGC 7 cut(s) 43, 1511, 1681, 2302, 2521, 2854, 4038
HpySE526I ACGT 7 cut(s) 131, 943, 1153, 1657, 1762, 3416, 3614
Hsp92I GRCGYC 4 cut(s) 99, 162, 443, 3416
HspAI GCGC 4 cut(s) 277, 912, 1972, 2383
Kpn2I TCCGGA 1 cut(s) 3925
Ksp22I TGATCA 1 cut(s) 295
LmnI GCTCC 8 cut(s) 146, 385, 971, 2302, 2923, 3085, 3803, 3975
LweI GCATC 6 cut(s) 646, 2125, 2145, 2161, 4106, 4163
MaeI CTAG 7 cut(s) 113, 2024, 2222, 3011, 3179, 3326, 4163
MaeII ACGT 7 cut(s) 131, 943, 1153, 1657, 1762, 3416, 3614
MaeIII GTNAC 8 cut(s) 515, 721, 857, 934, 1116, 1220, 1827, 2277
MbiI CCGCTC 3 cut(s) 149, 263, 388
MfeI CAATTG 5 cut(s) 344, 2401, 2429, 3050, 3373
MflI RGATCY 3 cut(s) 3549, 3909, 4143
MhlI GDGCHC 4 cut(s) 571, 1588, 1770, 2307
MlsI TGGCCA 1 cut(s) 3435
MluNI TGGCCA 1 cut(s) 3435
MlyI GAGTC 7 cut(s) 618, 749, 1129, 1814, 2527, 2825, 3879
Mox20I TGGCCA 1 cut(s) 3435
Mph1103I ATGCAT 2 cut(s) 1042, 1485
MroI TCCGGA 1 cut(s) 3925
MroXI GAANNNNTTC 1 cut(s) 2571
MscI TGGCCA 1 cut(s) 3435
MslI CAYNNNNRTG 2 cut(s) 651, 2595
Msp20I TGGCCA 1 cut(s) 3435
MspA1I CMGCKG 4 cut(s) 40, 2264, 2789, 3484
MspI CCGG 4 cut(s) 94, 606, 1232, 3926
MspR9I CCNGG 5 cut(s) 95, 606, 1232, 1696, 3398
MunI CAATTG 5 cut(s) 344, 2401, 2429, 3050, 3373
Mva1269I GAATGC 4 cut(s) 1040, 2016, 2399, 2473
MvaI CCWGG 2 cut(s) 1696, 3398
MvnI CGCG 3 cut(s) 66, 460, 1410
MwoI GCNNNNNNNGC 7 cut(s) 43, 1511, 1681, 2302, 2521, 2854, 4038
NciI CCSGG 3 cut(s) 95, 606, 1232
NlaIV GGNNCC 1 cut(s) 757
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 5 cut(s) 515, 721, 857, 934, 1220
NruI TCGCGA 2 cut(s) 66, 460
NsiI ATGCAT 2 cut(s) 1042, 1485
NspI RCATGY 5 cut(s) 247, 604, 1040, 2278, 3742
NspV TTCGAA 2 cut(s) 1087, 1579
OliI CACNNNNGTG 1 cut(s) 2595
PaeI GCATGC 1 cut(s) 604
PaqCI CACCTGC 1 cut(s) 1580
PciI ACATGT 2 cut(s) 243, 2274
PctI GAATGC 4 cut(s) 1040, 2016, 2399, 2473
PdmI GAANNNNTTC 1 cut(s) 2571
PflMI CCANNNNNTGG 3 cut(s) 1701, 2423, 3197
PfoI TCCNGGA 1 cut(s) 3396
PleI GAGTC 7 cut(s) 617, 748, 1129, 1813, 2527, 2824, 3879
PmaCI CACGTG 1 cut(s) 944
PmlI CACGTG 1 cut(s) 944
PpsI GAGTC 7 cut(s) 617, 748, 1129, 1813, 2527, 2824, 3879
Ppu21I YACGTR 2 cut(s) 944, 1763
PscI ACATGT 2 cut(s) 243, 2274
PshAI GACNNNNGTC 1 cut(s) 3198
PshBI ATTAAT 3 cut(s) 1053, 1965, 2910
PsiI TTATAA 1 cut(s) 336
Psp124BI GAGCTC 1 cut(s) 2307
Psp1406I AACGTT 2 cut(s) 1657, 3614
Psp6I CCWGG 2 cut(s) 1694, 3396
PspCI CACGTG 1 cut(s) 944
PspEI GGTNACC 1 cut(s) 1116
PspGI CCWGG 2 cut(s) 1694, 3396
PspN4I GGNNCC 1 cut(s) 757
PspPI GGNCC 6 cut(s) 126, 755, 1405, 2181, 2218, 3955
PstI CTGCAG 1 cut(s) 39
PstNI CAGNNNCTG 1 cut(s) 43
PsuI RGATCY 3 cut(s) 3549, 3909, 4143
PvuII CAGCTG 3 cut(s) 40, 2789, 3484
RruI TCGCGA 2 cut(s) 66, 460
RsaI GTAC 6 cut(s) 321, 1236, 1757, 2602, 3386, 3470
RsaNI GTAC 6 cut(s) 320, 1235, 1756, 2601, 3385, 3469
RseI CAYNNNNRTG 2 cut(s) 651, 2595
SacI GAGCTC 1 cut(s) 2307
Sau96I GGNCC 6 cut(s) 126, 755, 1405, 2181, 2218, 3955
ScaI AGTACT 2 cut(s) 1757, 3386
SchI GAGTC 7 cut(s) 618, 749, 1129, 1814, 2527, 2825, 3879
ScrFI CCNGG 5 cut(s) 95, 606, 1232, 1696, 3398
SduI GDGCHC 4 cut(s) 571, 1588, 1770, 2307
SfaNI GCATC 6 cut(s) 646, 2125, 2145, 2161, 4106, 4163
SfcI CTRYAG 1 cut(s) 35
SfuI TTCGAA 2 cut(s) 1087, 1579
SinI GGWCC 4 cut(s) 126, 755, 2181, 2218
SmiMI CAYNNNNRTG 2 cut(s) 651, 2595
SmlI CTYRAG 7 cut(s) 737, 2140, 2297, 2537, 3380, 3461, 3758
SmoI CTYRAG 7 cut(s) 737, 2140, 2297, 2537, 3380, 3461, 3758
SpeI ACTAGT 1 cut(s) 3325
SphI GCATGC 1 cut(s) 604
SsiI CCGC 8 cut(s) 26, 149, 261, 388, 1408, 2124, 2262, 2522
SspI AATATT 4 cut(s) 1026, 1185, 1712, 3060
SspMI CTAG 7 cut(s) 113, 2024, 2222, 3011, 3179, 3326, 4163
SstI GAGCTC 1 cut(s) 2307
StyD4I CCNGG 5 cut(s) 93, 604, 1230, 1694, 3396
StyI CCWWGG 1 cut(s) 2889
TaaI ACNGT 6 cut(s) 1256, 1749, 3533, 3632, 3782, 3934
TaiI ACGT 7 cut(s) 134, 946, 1156, 1660, 1765, 3419, 3617
TatI WGTACW 2 cut(s) 1755, 3384
TauI GCSGC 3 cut(s) 29, 263, 1410
TscAI CASTG 7 cut(s) 12, 1259, 1819, 2478, 3536, 3635, 3994
TseFI GTSAC 5 cut(s) 515, 721, 857, 934, 1220
Tsp45I GTSAC 5 cut(s) 515, 721, 857, 934, 1220
TspGWI ACGGA 5 cut(s) 337, 398, 424, 682, 2340
TspRI CASTG 7 cut(s) 12, 1259, 1819, 2478, 3536, 3635, 3994
Van91I CCANNNNNTGG 3 cut(s) 1701, 2423, 3197
VpaK11BI GGWCC 4 cut(s) 126, 755, 2181, 2218
VspI ATTAAT 3 cut(s) 1053, 1965, 2910
XapI RAATTY 6 cut(s) 254, 1072, 1267, 1316, 2333, 3263
XceI RCATGY 5 cut(s) 247, 604, 1040, 2278, 3742
XmiI GTMKAC 3 cut(s) 137, 466, 4117
XmnI GAANNNNTTC 1 cut(s) 2571
XspI CTAG 7 cut(s) 113, 2024, 2222, 3011, 3179, 3326, 4163
ZraI GACGTC 1 cut(s) 3417
ZrmI AGTACT 2 cut(s) 1757, 3386
Zsp2I ATGCAT 2 cut(s) 1042, 1485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.