FvH4_7g02450

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000070
Physical Location & Seq
Reverse (-)
2725490 .. 2729734
4245 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g02450.t1

Sequence Viewer

Length: 3912 bp
ATGGTGGTGGAGGTGTTTCTGGGCGCTTTTGTTCATTTGCTGCTCGAGAGGTTGGCCCCCCACAACTTGCTCAGTTTTGGTCAATTCCACGGCGTCGAGAAGAAGCTCAAGAAATGGAGTGCCACTCTGTCAGCAATTGGAGCTGTGCTGCAGGATGCTGAGAAGAAGCAACTCACAAGCGAGGCCGTGAAGCTGTGGCTGGATGAGCTCATACACTTGGCTTTTGACATGGATGACATCTTGGACACCTTTTCTACCCAGCTTTTGGTGATCAATCACCAAAATGGGGCTGGTACAAGCAAGGTACGACGCCTCTTTTCGGGAGTCAAGTTCAACTTTAACATGAACTTGGAAATAAATGACATTGCTGACCGATTAAAAGTAGTTTCTGATAGAAAAGATAAGCTGGGTTTAGTGTATAGTGATGATCCATATCACCCTCGGGCATCACAAAGGTTGCTGAGTTCATATGTGTTGGATGGACCTGTGGTTGGAAGGGAGGATGACAAGAGAAAGATAGTTGACTTGTTGTTAAGAGATCATGAGCCTCGTTGTGCCATCAATTTCCAAGTCTTGGCCATTGTTGGGATGCCGGGACTTGGCAAGACCACACTTGCAGGGCATGTGTTTAATGACAAGGAAATGAGGCAGTTCAATCCAAAGGTGTGGGTATCTGTTTCTGATGGCTTCAGTCTAGAGAGAGTTACGAAAACAATTTTCAAGTTAGTCACATCCCGGGATTCTGATGATTTAGATGAGTTCAGTAAAGTGCAGGATCTTCTGAGTGAGGCATTGGCAGGCAAAAAGTTTCTAATTGTTTTGGATGATGTTTGGAGTACTTGTGACTATGATTCATGGACAAAACTCCAGTCTCCCTTTCGTGTTGGAGCACCAGGAAGTCAGATACTGGTGACTACTCGTGAAGAAAAAGTAGGAAAATTGATAGGAGCCACTCAGGTTTATGATTTGAAGACCCTATCAGATGAGGATTGTTTGGAAGTATTTCAGCAACATATTAACAGTAATAGACCACCAAATTTTAATCTTCTTTGCAAGAAGATTGTTGAAAAGTGCAGTGGATTGCCATTGGCTGCAAAGACTCTTGGTGGTATTTTACGCTGTAAGGAAGCTGGCAGTTGGGAAGAAGTATTGGATGATAAATTGTGGAGTACATCAAATAGTGAGAGCAATGTTCTCACAGTACTGAAATTGAGCTATCATTATCTTCCTTCGAATTTGAAGAGGTGCTTTGCATATTGCTCAATACTTCCAAATCAATACGAATTCGGGAAAAAGCAGTTAATAGTTCTATGGATGGCAGAGGGTTTTCTTCATCAATCAGGAAGCAAGGAGATGGAAGATATTGGTGGTGACTATTTTGCGGAGCTATTGTCTCGGTCATTGTTTCAGAAGTCAAGCAAAAATAATTCAAGATATGAAATGCATGACCTTGTTGGTGATTTGGCACGGTGGGCTGCAGGAGATATATGTTTTAGATTGGAGGATAAGCTGGATGGAAAATGTTCTCCAAAGACTCGTCATGCAACTTATATTTCTGGCAAGTTTGATGGGGTAAAAAAATTTGAAGCCTTCTCTAAAGCCAAACGTTTGCGGACTTTCTTACCAGTTTCGGTTTCATATGGTCATGAGAATTATCAAACTCGTAAGGTTGCTTCTGATTTATTACCAAATATGAAGTACTTACGGGTGCTCTCTTTTAATGGCTATCAAATGACTGAACTGCCGGATTCAATTGGTAGACTAAAGCATCTAAGGTACCTTGATCTTTCTTACACATTAATATTGTGTTTGCCTGATTCAATATGCTCTCTTTACAACTTACAGACGTTGATATTGGAACATTGTTCTAAATTGAAGGCACTACCTTCAAACATGAGTAATTTAAGTAATTTACGTCATCTCAACAATTCTAGCATGCTTTCTTTGGAAGGAATGCCTCCCCAGTTAGGTCAATTGACTAGCCTGCAAACATTATCCTGTTTTGTGGTGGGCAAAGCTAGGGTGTCAGGTGTGAAGGAGATAGGTCCCTTAAATCTTCTTCGTGGGACATTATGCCTCTCAAGATTAGAAAATGTCAGTGATGTGGAGGATGCAAGACAGGCTGACTTGATGCGCAAGGAAGGGCTTGATGCATTGCAACTAGAATGGAGTGGCAGAGGAGAAAAAGAACCAGATGTGCTTGACATGGTAAAACCTCATAGAAACCTCAAAGAGCTTACCATAAAAGGCTATGGGGGTTTGGAATTGCCATCATGGATATGTAATCCTATATTCTCTGTTCTGGTGCTTTTGAGGTTAGAGAATTGTAATAATTGTCAATTCTTGCCACAACTTGGCCAATTACCGTCTCTCAAGGAACTCTTCATAAGAGGAATGTCTGAAGTAATAAGTGTTGGTGTTGAGTTTTATGGAGAGGGTACCTTATCTTTTCCAGTACTGGAGAATCTTGTGTTTGAGGATATGCAACAATGGAAGGGATGGTTTCCTTGTGAAGAAGATCAAATTGGAGTGTTTCCTTCCTTGAAAATGCTTTCTGTCATTAGGTGTCCCCAATTGGTTGTCTCTGTTTTAAACTACAATCAACTTTGTGCATTACAAGTCCATGACTGCTACGGGGTGGTATTTAGAGGTGCTGTTGGCTTTGAGTTACTTGAGCATATGTATCTTTCAAATTTGTCAGTGTTGAGACTTGAAATTGAGAAATCTGCAGAATGCATGAGGGGCTTCGGAAATGTCAAAGGTTTGGGAATTACTAATAGTGAGGAATCATCATCTTCCTGGCAGAACGAGGATATAATACTGCAGCACCTGAGTTCTCTTCGTAGTTTGTTTATTGAAAGGAACTCCAAGCTTCTTCAATTGCATCACCTGACATCACTTCAAGCGCTTCGCTTATATAGATGTGAAAGTCTAGTTTCTATTCCGGAAACATGTTTGCCACCTTCTCTTAAAGATCTGTGGATTGAAAGGTGCGATTCTCTTACCTGTTTTGCAAGTTACCAGATACCTCCAAGTCTAAGAAGAGTAGGAATAAGAGAATGCAGAAGGTTGAAATTACTAGTTGGGGAGAATGTAGAAGGTTCCTCATCCTCTTCTTCTTCTCACTATTTGATGCAAGAGGATACATCTTGTCTCGAGTACTTGGAGGTTTGGGAATGTCCATCTCTTGCATCCTTATCATCCAGAGGCCAACTACCCAGGACACTTAAACACCTTCACATAAGGGATTGTGAACGACTGGAGTCAATAACAAATATGTTCCACAGGGAAACTTGTCTTGAGCATATTGTGATACAGAGTTGTGCAAACCTTAAATCCTTACCAGACGGCCTATGCGACCTCACTCATCTTCAACAGTTAATTGTTATGGATTGTGGAAGTCTTCTATCCTTCCCGAGTGGAGGGTTACCAACAGCAGCCTCCAACCTGACAAGCATCTTTGTCTACAATTGTGATAGCTTGGAAGCTTTCCCCAGAGGAATGGACAAGCACATCTCTCTTCAGACATTGTGGATTGGTTACTGTGAAGGTTTGGCATCCATTCTAGAAGAGGGTTTTTCCCCAAACCTAGTTGAGCTTTACATTGTGAACCCTTTACGTTGCAAGCCGCTCTCTGAATGGGGGTTGCAGTTGCACAGACTCAATTCCTTGAAAGAGTTGTGGATCCAGGGTGTTGATCCAAAACTGGTATCATTTCCACCGGAGGAGATGGAGATGCGGCTACCTAAATCTCTCATTAAAATCAGAATTGGAGACTTCCCAAAGCTAAGAAGAGATACAGACCAGGAAAAGCGCCACACTGGCCCAAAATATGCCACATTCCTTACATACAGATCGGATAGTACACACCAAAAGGCTTGTAACCGGAATCCCTTCTCCCCAGGTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1304

Amino Acids

147.22

Weight (kDa)

6.58

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 11 - 90 5.8e-19 Rx N-terminal domain
NB-ARC PF00931 167 - 342 4.2e-34 NB-ARC domain
WHD_DRP PF23559 422 - 489 8.6e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 562 - 853 3.8e-31 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 679 - 799 8.6e-35 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 1047 - 1183 3.2e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2135
Acc65I GGTACC 2 cut(s) 1776, 2438
AccB1I GGYRCC 2 cut(s) 1776, 2438
AccB7I CCANNNNNTGG 3 cut(s) 265, 574, 2354
AccBSI CCGCTC 1 cut(s) 3631
AccI GTMKAC 2 cut(s) 1759, 3465
AccIII TCCGGA 1 cut(s) 2944
AciI CCGC 4 cut(s) 1382, 1612, 3629, 3739
AclI AACGTT 1 cut(s) 1606
AclWI GGATC 5 cut(s) 422, 783, 3679, 3692, 3692
AcoI YGGCCR 2 cut(s) 576, 2356
AcsI RAATTY 5 cut(s) 1036, 1234, 1283, 1580, 2692
AcuI CTGAAG 3 cut(s) 673, 2421, 3506
AcyI GRCGYC 2 cut(s) 93, 310
AfeI AGCGCT 1 cut(s) 2907
AflIII ACRYGT 1 cut(s) 2951
AhlI ACTAGT 1 cut(s) 3079
AjnI CCWGG 6 cut(s) 892, 2798, 3218, 3687, 3804, 3901
AjuI GAANNNNNNNTTGG 2 cut(s) 2508, 2540
Alw21I GWGCWC 3 cut(s) 210, 892, 1713
Alw26I GTCTC 7 cut(s) 876, 1398, 2373, 2587, 2701, 3158, 3768
AlwI GGATC 5 cut(s) 422, 783, 3679, 3692, 3692
AlwNI CAGNNNCTG 2 cut(s) 907, 2830
Ama87I CYCGRG 5 cut(s) 44, 441, 735, 3155, 3415
Aor13HI TCCGGA 1 cut(s) 2944
Aor51HI AGCGCT 1 cut(s) 2907
AoxI GGCC 7 cut(s) 54, 183, 576, 2356, 3208, 3349, 3823
ApeKI GCWGC 6 cut(s) 40, 148, 1091, 1475, 2824, 3437
ApoI RAATTY 5 cut(s) 1036, 1234, 1283, 1580, 2692
ArsI GACNNNNNNTTYG 2 cut(s) 854, 886
AseI ATTAAT 1 cut(s) 1799
Asp700I GAANNNNTTC 3 cut(s) 1002, 1522, 3893
Asp718I GGTACC 2 cut(s) 1776, 2438
AspLEI GCGC 4 cut(s) 26, 2136, 2908, 3816
AspS9I GGNCC 4 cut(s) 55, 482, 2045, 3824
AsuC2I CCSGG 3 cut(s) 594, 736, 737
AsuHPI GGTGA 7 cut(s) 269, 280, 428, 922, 1382, 1469, 2879
AsuII TTCGAA 1 cut(s) 1232
AvaI CYCGRG 5 cut(s) 44, 441, 735, 3155, 3415
AvaII GGWCC 2 cut(s) 482, 2045
BalI TGGCCA 2 cut(s) 578, 2358
BamHI GGATCC 1 cut(s) 3684
BanI GGYRCC 2 cut(s) 1776, 2438
BanII GRGCYC 1 cut(s) 210
BarI GAAGNNNNNNTAC 2 cut(s) 1657, 1689
BauI CACGAG 1 cut(s) 918
BbsI GAAGAC 2 cut(s) 977, 3395
Bbv12I GWGCWC 3 cut(s) 210, 892, 1713
BbvI GCAGC 6 cut(s) 27, 135, 1078, 1462, 2836, 3449
BceAI ACGGC 3 cut(s) 106, 170, 3364
BciT130I CCWGG 6 cut(s) 894, 2800, 3220, 3689, 3806, 3903
BciVI GTATCC 1 cut(s) 3136
BclI TGATCA 1 cut(s) 270
BcnI CCSGG 3 cut(s) 594, 736, 737
BcoDI GTCTC 7 cut(s) 876, 1398, 2373, 2587, 2701, 3158, 3768
BcuI ACTAGT 1 cut(s) 3079
BfaI CTAG 9 cut(s) 695, 1932, 1980, 2019, 2162, 2933, 3080, 3566, 3590
BfmI CTRYAG 4 cut(s) 149, 1476, 2727, 2822
BfoI RGCGCY 3 cut(s) 27, 2909, 3817
BfuI GTATCC 1 cut(s) 3136
BglI GCCNNNNNGGC 1 cut(s) 3822
BglII AGATCT 1 cut(s) 2974
BisI GCNGC 8 cut(s) 41, 149, 1092, 1476, 2825, 3438, 3629, 3740
BlsI GCNGC 8 cut(s) 42, 150, 1093, 1477, 2826, 3439, 3630, 3741
BmcAI AGTACT 5 cut(s) 838, 1203, 1700, 2457, 3161
Bme1390I CCNGG 9 cut(s) 594, 736, 737, 894, 2800, 3220, 3689, 3806, 3903
Bme18I GGWCC 2 cut(s) 482, 2045
BmeT110I CYCGRG 5 cut(s) 44, 441, 735, 3155, 3415
BmgT120I GGNCC 4 cut(s) 55, 482, 2045, 3824
BmiI GGNNCC 7 cut(s) 57, 949, 1778, 2047, 2440, 3103, 3686
BmrFI CCNGG 9 cut(s) 594, 736, 737, 894, 2800, 3220, 3689, 3806, 3903
BmrI ACTGGG 1 cut(s) 1957
BmuI ACTGGG 1 cut(s) 1957
BoxI GACNNNNGTC 1 cut(s) 3262
BpiI GAAGAC 2 cut(s) 977, 3395
BplI GAGNNNNNCTC 2 cut(s) 109, 141
BpmI CTGGAG 3 cut(s) 851, 2480, 3281
Bpu14I TTCGAA 1 cut(s) 1232
BpuEI CTTGAG 5 cut(s) 92, 2065, 2357, 2693, 3320
BpuMI CCSGG 3 cut(s) 594, 736, 737
BsaBI GATNNNNATC 1 cut(s) 432
BsaHI GRCGYC 2 cut(s) 93, 310
BsaJI CCNNGG 6 cut(s) 88, 440, 735, 3218, 3688, 3901
BsaWI WCCGGW 3 cut(s) 2944, 3721, 3885
BsaXI ACNNNNNCTCC 4 cut(s) 826, 856, 1376, 1406
Bse1I ACTGG 9 cut(s) 868, 912, 1625, 1963, 2453, 2463, 3264, 3711, 3826
Bse3DI GCAATG 3 cut(s) 363, 1195, 2153
Bse8I GATNNNNATC 1 cut(s) 432
BseAI TCCGGA 1 cut(s) 2944
BseBI CCWGG 6 cut(s) 894, 2800, 3220, 3689, 3806, 3903
BseDI CCNNGG 6 cut(s) 88, 440, 735, 3218, 3688, 3901
BseJI GATNNNNATC 1 cut(s) 432
BseMI GCAATG 3 cut(s) 363, 1195, 2153
BseMII CTCAG 6 cut(s) 85, 150, 452, 773, 968, 2822
BseNI ACTGG 9 cut(s) 868, 912, 1625, 1963, 2453, 2463, 3264, 3711, 3826
BseRI GAGGAG 2 cut(s) 2193, 3740
BseXI GCAGC 6 cut(s) 27, 135, 1078, 1462, 2836, 3449
BseYI CCCAGC 2 cut(s) 258, 406
BsgI GTGCAG 2 cut(s) 791, 1093
BshFI GGCC 7 cut(s) 56, 185, 578, 2358, 3210, 3351, 3825
BshNI GGYRCC 2 cut(s) 1776, 2438
BsiHKAI GWGCWC 3 cut(s) 210, 892, 1713
BsiHKCI CYCGRG 5 cut(s) 44, 441, 735, 3155, 3415
BsiSI CCGG 6 cut(s) 593, 736, 1745, 2945, 3722, 3886
BslFI GGGAC 4 cut(s) 609, 2031, 2080, 2553
BsmAI GTCTC 7 cut(s) 876, 1398, 2373, 2587, 2701, 3158, 3768
BsmBI CGTCTC 1 cut(s) 2373
BsmFI GGGAC 4 cut(s) 609, 2031, 2080, 2553
BsmI GAATGC 3 cut(s) 1959, 2738, 3065
BsnI GGCC 7 cut(s) 56, 185, 578, 2358, 3210, 3351, 3825
BsoBI CYCGRG 5 cut(s) 44, 441, 735, 3155, 3415
Bsp119I TTCGAA 1 cut(s) 1232
Bsp1286I GDGCHC 3 cut(s) 210, 892, 1713
Bsp13I TCCGGA 1 cut(s) 2944
BspACI CCGC 4 cut(s) 1382, 1612, 3629, 3739
BspANI GGCC 7 cut(s) 56, 185, 578, 2358, 3210, 3351, 3825
BspCNI CTCAG 6 cut(s) 84, 151, 453, 774, 967, 2823
BspEI TCCGGA 1 cut(s) 2944
BspHI TCATGA 2 cut(s) 541, 1645
BspLI GGNNCC 7 cut(s) 57, 949, 1778, 2047, 2440, 3103, 3686
BspMAI CTGCAG 4 cut(s) 153, 1480, 2731, 2826
BspPI GGATC 5 cut(s) 422, 783, 3679, 3692, 3692
BspT104I TTCGAA 1 cut(s) 1232
BspT107I GGYRCC 2 cut(s) 1776, 2438
BsrBI CCGCTC 1 cut(s) 3631
BsrDI GCAATG 3 cut(s) 363, 1195, 2153
BsrI ACTGG 9 cut(s) 868, 912, 1625, 1963, 2453, 2463, 3264, 3711, 3826
BssECI CCNNGG 6 cut(s) 88, 440, 735, 3218, 3688, 3901
BssNI GRCGYC 2 cut(s) 93, 310
BssSI CACGAG 1 cut(s) 918
Bst2BI CACGAG 1 cut(s) 918
Bst2UI CCWGG 6 cut(s) 894, 2800, 3220, 3689, 3806, 3903
Bst4CI ACNGT 6 cut(s) 1022, 1201, 1470, 2367, 3378, 3545
Bst6I CTCTTC 8 cut(s) 1235, 2387, 2844, 3037, 3118, 3525, 3564, 3787
BstACI GRCGYC 2 cut(s) 93, 310
BstBI TTCGAA 1 cut(s) 1232
BstC8I GCNNGC 5 cut(s) 799, 1132, 1937, 1985, 3626
BstDEI CTNAG 9 cut(s) 71, 159, 461, 782, 954, 1772, 2831, 3038, 3788
BstDSI CCRYGG 1 cut(s) 88
BstEII GGTNACC 1 cut(s) 3426
BstH2I RGCGCY 3 cut(s) 27, 2909, 3817
BstHHI GCGC 4 cut(s) 26, 2136, 2908, 3816
BstMAI GTCTC 7 cut(s) 876, 1398, 2373, 2587, 2701, 3158, 3768
BstMWI GCNNNNNNNGC 6 cut(s) 140, 205, 1472, 2120, 2742, 3822
BstNI CCWGG 6 cut(s) 894, 2800, 3220, 3689, 3806, 3903
BstNSI RCATGY 3 cut(s) 626, 1939, 2955
BstPAI GACNNNNGTC 1 cut(s) 3262
BstPI GGTNACC 1 cut(s) 3426
BstSCI CCNGG 9 cut(s) 592, 734, 735, 892, 2798, 3218, 3687, 3804, 3901
BstSFI CTRYAG 4 cut(s) 149, 1476, 2727, 2822
BstV1I GCAGC 6 cut(s) 27, 135, 1078, 1462, 2836, 3449
BstV2I GAAGAC 2 cut(s) 977, 3395
BstX2I RGATCY 3 cut(s) 775, 2974, 3684
BstXI CCANNNNNNTGG 2 cut(s) 666, 3502
BstYI RGATCY 3 cut(s) 775, 2974, 3684
BsuI GTATCC 1 cut(s) 3136
BsuRI GGCC 7 cut(s) 56, 185, 578, 2358, 3210, 3351, 3825
BtgI CCRYGG 1 cut(s) 88
BtsI GCAGTG 1 cut(s) 1081
BtsIMutI CAGTG 4 cut(s) 1081, 2104, 2706, 3819
Cac8I GCNNGC 5 cut(s) 799, 1132, 1937, 1985, 3626
CaiI CAGNNNCTG 2 cut(s) 907, 2830
CciI TCATGA 2 cut(s) 541, 1645
CfoI GCGC 4 cut(s) 26, 2136, 2908, 3816
Cfr13I GGNCC 4 cut(s) 55, 482, 2045, 3824
Cfr9I CCCGGG 1 cut(s) 735
CseI GACGC 2 cut(s) 82, 318
DdeI CTNAG 9 cut(s) 71, 159, 461, 782, 954, 1772, 2831, 3038, 3788
DraI TTTAAA 1 cut(s) 2592
EaeI YGGCCR 2 cut(s) 576, 2356
Eam1104I CTCTTC 8 cut(s) 1235, 2387, 2844, 3037, 3118, 3525, 3564, 3787
EarI CTCTTC 8 cut(s) 1235, 2387, 2844, 3037, 3118, 3525, 3564, 3787
Ecl136II GAGCTC 1 cut(s) 208
Eco24I GRGCYC 1 cut(s) 210
Eco47I GGWCC 2 cut(s) 482, 2045
Eco47III AGCGCT 1 cut(s) 2907
Eco53kI GAGCTC 1 cut(s) 208
Eco57I CTGAAG 3 cut(s) 673, 2421, 3506
Eco88I CYCGRG 5 cut(s) 44, 441, 735, 3155, 3415
Eco91I GGTNACC 1 cut(s) 3426
EcoICRI GAGCTC 1 cut(s) 208
EcoO109I RGGNCCY 1 cut(s) 2045
EcoO65I GGTNACC 1 cut(s) 3426
EcoRI GAATTC 1 cut(s) 1283
EcoRII CCWGG 6 cut(s) 892, 2798, 3218, 3687, 3804, 3901
EcoT22I ATGCAT 3 cut(s) 1446, 2155, 2738
EcoT38I GRGCYC 1 cut(s) 210
Esp3I CGTCTC 1 cut(s) 2373
FalI AAGNNNNNCTT 6 cut(s) 320, 352, 1232, 1264, 2366, 2398
FaqI GGGAC 4 cut(s) 609, 2031, 2080, 2553
FauNDI CATATG 3 cut(s) 469, 1639, 2679
FbaI TGATCA 1 cut(s) 270
FblI GTMKAC 2 cut(s) 1759, 3465
Fnu4HI GCNGC 8 cut(s) 41, 149, 1092, 1476, 2825, 3438, 3629, 3740
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 8 cut(s) 41, 149, 1092, 1476, 2825, 3438, 3629, 3740
FspBI CTAG 9 cut(s) 695, 1932, 1980, 2019, 2162, 2933, 3080, 3566, 3590
FspI TGCGCA 1 cut(s) 2135
GlaI GCGC 4 cut(s) 25, 2135, 2907, 3815
GluI GCNGC 8 cut(s) 41, 149, 1092, 1476, 2825, 3438, 3629, 3740
GsaI CCCAGC 2 cut(s) 262, 410
GsuI CTGGAG 3 cut(s) 851, 2480, 3281
HaeII RGCGCY 3 cut(s) 27, 2909, 3817
HaeIII GGCC 7 cut(s) 56, 185, 578, 2358, 3210, 3351, 3825
HapII CCGG 6 cut(s) 593, 736, 1745, 2945, 3722, 3886
HgaI GACGC 2 cut(s) 82, 318
HhaI GCGC 4 cut(s) 26, 2136, 2908, 3816
Hin1I GRCGYC 2 cut(s) 93, 310
Hin6I GCGC 4 cut(s) 24, 2134, 2906, 3814
HinP1I GCGC 4 cut(s) 24, 2134, 2906, 3814
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HindIII AAGCTT 2 cut(s) 2870, 3486
HpaII CCGG 6 cut(s) 593, 736, 1745, 2945, 3722, 3886
HphI GGTGA 7 cut(s) 269, 280, 428, 922, 1382, 1469, 2879
Hpy166II GTNNAC 6 cut(s) 523, 1760, 3254, 3466, 3610, 3866
Hpy8I GTNNAC 6 cut(s) 523, 1760, 3254, 3466, 3610, 3866
Hpy99I CGWCG 2 cut(s) 98, 312
HpyCH4III ACNGT 6 cut(s) 1022, 1201, 1470, 2367, 3378, 3545
HpyCH4IV ACGT 4 cut(s) 1606, 1847, 1915, 3619
HpyF10VI GCNNNNNNNGC 6 cut(s) 140, 205, 1472, 2120, 2742, 3822
HpyF3I CTNAG 9 cut(s) 71, 159, 461, 782, 954, 1772, 2831, 3038, 3788
HpySE526I ACGT 4 cut(s) 1606, 1847, 1915, 3619
Hsp92I GRCGYC 2 cut(s) 93, 310
HspAI GCGC 4 cut(s) 24, 2134, 2906, 3814
Kpn2I TCCGGA 1 cut(s) 2944
KpnI GGTACC 2 cut(s) 1780, 2442
Ksp22I TGATCA 1 cut(s) 270
LmnI GCTCC 4 cut(s) 140, 887, 947, 1384
Lsp1109I GCAGC 6 cut(s) 27, 135, 1078, 1462, 2836, 3449
MaeI CTAG 9 cut(s) 695, 1932, 1980, 2019, 2162, 2933, 3080, 3566, 3590
MaeII ACGT 4 cut(s) 1606, 1847, 1915, 3619
MbiI CCGCTC 1 cut(s) 3631
MfeI CAATTG 6 cut(s) 135, 1752, 1973, 2573, 2879, 3469
MflI RGATCY 3 cut(s) 775, 2974, 3684
MhlI GDGCHC 3 cut(s) 210, 892, 1713
MlsI TGGCCA 2 cut(s) 578, 2358
MluNI TGGCCA 2 cut(s) 578, 2358
MlyI GAGTC 5 cut(s) 333, 1093, 1528, 3272, 3654
MmeI TCCRAC 4 cut(s) 456, 472, 865, 3468
Mox20I TGGCCA 2 cut(s) 578, 2358
Mph1103I ATGCAT 3 cut(s) 1446, 2155, 2738
MroI TCCGGA 1 cut(s) 2944
MroXI GAANNNNTTC 3 cut(s) 1002, 1522, 3893
MscI TGGCCA 2 cut(s) 578, 2358
MslI CAYNNNNRTG 2 cut(s) 282, 2278
Msp20I TGGCCA 2 cut(s) 578, 2358
MspI CCGG 6 cut(s) 593, 736, 1745, 2945, 3722, 3886
MspR9I CCNGG 9 cut(s) 594, 736, 737, 894, 2800, 3220, 3689, 3806, 3903
MunI CAATTG 6 cut(s) 135, 1752, 1973, 2573, 2879, 3469
Mva1269I GAATGC 3 cut(s) 1959, 2738, 3065
MvaI CCWGG 6 cut(s) 894, 2800, 3220, 3689, 3806, 3903
MwoI GCNNNNNNNGC 6 cut(s) 140, 205, 1472, 2120, 2742, 3822
NciI CCSGG 3 cut(s) 594, 736, 737
NdeI CATATG 3 cut(s) 469, 1639, 2679
NlaIV GGNNCC 7 cut(s) 57, 949, 1778, 2047, 2440, 3103, 3686
NmuCI GTSAC 4 cut(s) 727, 842, 910, 1370
NsbI TGCGCA 1 cut(s) 2135
NsiI ATGCAT 3 cut(s) 1446, 2155, 2738
NspI RCATGY 3 cut(s) 626, 1939, 2955
NspV TTCGAA 1 cut(s) 1232
PaeI GCATGC 1 cut(s) 1939
PaeR7I CTCGAG 2 cut(s) 44, 3155
PagI TCATGA 2 cut(s) 541, 1645
PciI ACATGT 1 cut(s) 2951
PcsI WCGNNNNNNNCGW 1 cut(s) 3354
PctI GAATGC 3 cut(s) 1959, 2738, 3065
PdmI GAANNNNTTC 3 cut(s) 1002, 1522, 3893
PfeI GAWTC 8 cut(s) 740, 851, 1748, 1817, 2464, 2786, 2996, 3889
PflMI CCANNNNNTGG 3 cut(s) 265, 574, 2354
PkrI GCNGC 8 cut(s) 42, 150, 1093, 1477, 2826, 3439, 3630, 3741
PleI GAGTC 5 cut(s) 332, 1093, 1528, 3271, 3654
PpsI GAGTC 5 cut(s) 332, 1093, 1528, 3271, 3654
PpuMI RGGWCCY 1 cut(s) 2045
PscI ACATGT 1 cut(s) 2951
PshAI GACNNNNGTC 1 cut(s) 3262
PshBI ATTAAT 1 cut(s) 1799
Psp124BI GAGCTC 1 cut(s) 210
Psp1406I AACGTT 1 cut(s) 1606
Psp5II RGGWCCY 1 cut(s) 2045
Psp6I CCWGG 6 cut(s) 892, 2798, 3218, 3687, 3804, 3901
PspEI GGTNACC 1 cut(s) 3426
PspFI CCCAGC 2 cut(s) 258, 406
PspGI CCWGG 6 cut(s) 892, 2798, 3218, 3687, 3804, 3901
PspN4I GGNNCC 7 cut(s) 57, 949, 1778, 2047, 2440, 3103, 3686
PspPI GGNCC 4 cut(s) 55, 482, 2045, 3824
PspPPI RGGWCCY 1 cut(s) 2045
PstI CTGCAG 4 cut(s) 153, 1480, 2731, 2826
PstNI CAGNNNCTG 2 cut(s) 907, 2830
PsuI RGATCY 3 cut(s) 775, 2974, 3684
RseI CAYNNNNRTG 2 cut(s) 282, 2278
SacI GAGCTC 1 cut(s) 210
SatI GCNGC 8 cut(s) 41, 149, 1092, 1476, 2825, 3438, 3629, 3740
Sau96I GGNCC 4 cut(s) 55, 482, 2045, 3824
ScaI AGTACT 5 cut(s) 838, 1203, 1700, 2457, 3161
SchI GAGTC 5 cut(s) 333, 1093, 1528, 3272, 3654
ScrFI CCNGG 9 cut(s) 594, 736, 737, 894, 2800, 3220, 3689, 3806, 3903
SduI GDGCHC 3 cut(s) 210, 892, 1713
SfcI CTRYAG 4 cut(s) 149, 1476, 2727, 2822
Sfr274I CTCGAG 2 cut(s) 44, 3155
SfuI TTCGAA 1 cut(s) 1232
SinI GGWCC 2 cut(s) 482, 2045
SlaI CTCGAG 2 cut(s) 44, 3155
SmaI CCCGGG 1 cut(s) 737
SmiMI CAYNNNNRTG 2 cut(s) 282, 2278
SmlI CTYRAG 7 cut(s) 44, 107, 2080, 2372, 2672, 3155, 3299
SmoI CTYRAG 7 cut(s) 44, 107, 2080, 2372, 2672, 3155, 3299
SpeI ACTAGT 1 cut(s) 3079
SphI GCATGC 1 cut(s) 1939
SsiI CCGC 4 cut(s) 1382, 1612, 3629, 3739
SspI AATATT 1 cut(s) 1803
SspMI CTAG 9 cut(s) 695, 1932, 1980, 2019, 2162, 2933, 3080, 3566, 3590
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 9 cut(s) 592, 734, 735, 892, 2798, 3218, 3687, 3804, 3901
TaaI ACNGT 6 cut(s) 1022, 1201, 1470, 2367, 3378, 3545
TaiI ACGT 4 cut(s) 1609, 1850, 1918, 3622
TaqI TCGA 4 cut(s) 45, 96, 1232, 3156
TaqII GACCGA 2 cut(s) 387, 1386
TatI WGTACW 7 cut(s) 836, 1169, 1201, 1698, 2455, 3159, 3863
TauI GCSGC 2 cut(s) 3631, 3742
TfiI GAWTC 8 cut(s) 740, 851, 1748, 1817, 2464, 2786, 2996, 3889
TscAI CASTG 4 cut(s) 1081, 2104, 2706, 3826
TseFI GTSAC 4 cut(s) 727, 842, 910, 1370
TseI GCWGC 6 cut(s) 40, 148, 1091, 1475, 2824, 3437
Tsp45I GTSAC 4 cut(s) 727, 842, 910, 1370
TspDTI ATGAA 9 cut(s) 23, 359, 456, 843, 1322, 1452, 1626, 1709, 2374
TspMI CCCGGG 1 cut(s) 735
TspRI CASTG 4 cut(s) 1081, 2104, 2706, 3826
Van91I CCANNNNNTGG 3 cut(s) 265, 574, 2354
VpaK11BI GGWCC 2 cut(s) 482, 2045
VspI ATTAAT 1 cut(s) 1799
XapI RAATTY 5 cut(s) 1036, 1234, 1283, 1580, 2692
XbaI TCTAGA 2 cut(s) 694, 3565
XceI RCATGY 3 cut(s) 626, 1939, 2955
XcmI CCANNNNNNNNNTGG 1 cut(s) 287
XhoI CTCGAG 2 cut(s) 44, 3155
XmaI CCCGGG 1 cut(s) 735
XmiI GTMKAC 2 cut(s) 1759, 3465
XmnI GAANNNNTTC 3 cut(s) 1002, 1522, 3893
XspI CTAG 9 cut(s) 695, 1932, 1980, 2019, 2162, 2933, 3080, 3566, 3590
ZrmI AGTACT 5 cut(s) 838, 1203, 1700, 2457, 3161
Zsp2I ATGCAT 3 cut(s) 1446, 2155, 2738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.