Rw2G028210

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000070
Physical Location & Seq
Forward (+)
44973299 .. 44977546
4248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G028210.1

Sequence Viewer

Length: 4143 bp
ATGGCAGTGGTGGAGATCTTTCTGGCGTCATTCCTTAAGGTGCTGCTTAATAGGCTGACGGATGACTTCCTCAATTTTGCACGACAAGGGGGTATTGACAAGAAGCTGAAAAAATGGAAGGACATGCTGGCTGCAATCGGAGCTGTGCTGCATGATGCTGAAGAGAAGCAACTGACGAGCGAGGCAGTGAAACTGTGGCTAGATGATCTGAAAGACCTGGCTTATGATGTGGAAGATGTTTTGGATGAGTTTTCCACTGAGATGATACACCGCAAAGTCAAGGAGCAGCAATATGGGGCTACCATGAGCAAGGTACGAGGCTTCATTCCTAAAATTAGATTTAGCTTCAATTTTAACTCCAAGATAAAGCTTATTACTGATCAGTTACAAGACATATCTGAACGGAAAGACAAGCTTGGTTTGACTTATATTGGGACAATATCTACTAGTAAGGAATGGCTAAGGCCACCGAGTTCATGTGTGTTGGGTGGAACACCTGTTGTTGGAAGAGATGTGGACAAAGCAAAGATTGTTGAGTTTCTCTCAAGAGATGAGCCTAGCACAATCAATTTTCATGTTGTTGCCATTGTTGGTATGCCTGGATTGGGGAAAACAACACTTGCTCAGTATATATTCAATGACAGTGATGATGATGTCCTGAAACAGTTCAATCTTAAGGTCTGGGTATCAGTGTCGGATAACTTTAACATTGTAAGAGTAACTAAAGCAATTCTTGAATCAGTCACGTCCCAACACTGTCGATGGAAGGAATTCAGTCAAATTCAAGATGGCTTGAGAAAGGAACTAGCAAGTAAAAAGTATTTAATTGTTTTGGATGACATTTGGAACTCATGTGACTATGATCTATGGATGACACTACAGTCCCCTTTTCATGCTGGTGCACCAGGAAGTAAAATAATTGCGACAACACGTGATGCAGGAGTTGCAAAAATAGTGAGAGCCATTGATGTTCACAATTTGGACTATATATCAAATGATGATTGTTGGAAAGTATTCAAGCAACATGCACTCTTGGATGTTAGTAATTGCGGACCACAGAACTTTGAGGTATTTCGGGGGGAAATTGTTGCCAAATGCGGTGGATTACCTTTGGCTGCAAGAACTCTTGGTGGTCTTTTAGGTTGTAAAGAAATACATGAATGGGGAGAAATACTTGAAAACAAGCTGTGGAGTCTATCTGATAATAGTAACATTCTCCCCGTGTTAAATTTGAGCTATCATTATCTCCCTTCAGATTTGAAAAGGTGTTTTGCTTATTGCTCGATACTTCCACAAGATTATGAATTTGAGGAAATGCAGTTGATCCTCCTATGGATGGCAGAGGATTTAATTCCACAACCGAAAGACAACAAACAATTGGAAGATTTAGGTGCTGATTATTTTCGGGAGTTGTTATCTAGATCATTCTTTCAAAATTCAAGCAAAAGCAATGCACGATATGTAATGCATGATCTCATTAGTGATTTGGCAAGAAAGATCACAGGAGCGATATGTTTTAGATTGGAGGACGCACAGAATAGTTACAGGCAAATTAGATCTTCTCCGAAGGCTCGTCAATTGTCTTACATTTCTGGCATATCTGATGGTGTTAAAAAATTCGAAGCCTTTGATCAAGTCAAACATCTGCGGACTTTTCTACCATTTACTGTTTCAACACTGCCAGGTTTTCGACATTGTTTGACTCGTAAGATCACTCATGATTTAGTGCCAAAACTGCCATACCTAAGGGTGTTATCTTTTAATGGCTATGGAATCACAGATCTGCCAAAATCGATAGGGGAGTTGAAGTCTTTACGGTATCTTGATGTTTCTCATGCAGTAATAAAGGATTTTCCTGAATCTGCCAGTACTCTTTACAACTTACAAACATTGATAGTAAATGATTGTAATTTTTTGAGAACACTACCTACAAACATGAGGAATCTAATCAGTTTGCGTCATCTTCACAATTCGAAACTATATTTGTTGGAAAGAATGCCTGGCCAACTTGGTAGTCTGACAAATCTGCAAACATTGAATAATTTTCTGGTGGCCGAAAGTGGAGATTCAGGGATAAGTCAGATCGGTCCCCTATTGCATCTCCGGGGGACATTGCATCTTTCAAGATTGGAGAATGTGAATGATGCGGAGGATGCAAAGATGGCCTACTGTATTAAGAAGGACAAGCTTGATGAGTTGGTACTCGAATGGAGTGAAAACAGAGAAATGAGACTAGATGTGCTTGACATGTTGCAACCTCACAGAAACCTCAAAATGCTCACAGTAAGGGGTTTCAGGGGATTGCAATTTTCAGCATGGATTGGAGATTCTTTATTCTCTAAAATGGTGGTGGTGAGGTTAGAAAATTGTAGAAACTGCCAGCTCTTGCCACCACTTGGGCAATTGTGTTCTCTGAAAGAGCTATATATAAGAAAAATGCCTGGAGTGGTAAATGTAGGGCAGGAGTTTTATGGGGAGGGTAGCTTGCCTTTCCCTCGATTAGAAATTCTGGAATTTGAGCGGATGGACCTTTGGAAGGAGTGGTTTTCCTATCAACAAGATCAGGGACTTGAAACTTTCCCTTGCTTGAAAATGCTTTCCATCAAAAGCTGCTGGAAACTGCAGGGTCGGTTACCTGAGAACCTTCATTTACTGTCCACGCTTGTGATTCATCAATGTGAGCAGCTAGTGGTTTCAATCACAAATTATAAAAGCCTTTGTGAATTGAAAATAAATAATTGCAGACAGGTGATCCATAGAAGTGCTGCTCAGTTTGAGTCGCTAGAGGCCCTAGAGCTTTCAAGTGTTTCAGAGTTCGTACTCCAAGCAGAGGAATTTGTGAAAGGGTTGACAAAAACTAAAGATCTGAAGATTACAGTTTCTAAGAAGCCGACATTTTTAAGGGAGAATGAGGATAGATTACTGGAAAACCAGACTTCTGTTCCTCATTTCTTCATTGGAAGCAACTCCCACCTTGTTTTGTTTCATTACTTATCATCACTTCAGAAACTTCACATATACGACTGCCCTAGTATAATTTCTTTTGAAGAGGTTTGTTTTCCACCTTTTCTTGCAGAAATAGAGATTGTGCGTTGCCATTCTCTTGTGTATTTCGCAAGATACCAGATTTCCCCACAACTGAAAAGATTAAGTATCTGGGTTTGCAAAAATTTTAAATCAGTAGTAGAGATTGAAGTAGGAAGTACTCAGACTTCATCTTGTTGGTCTTCCTCATCTTCTGCTTCTACTCCTTGTATGATGCAAAAGGAGACAACTAGTCTCGAGGACTTGGAAATAATTAGTTGTCCCTCTATGACGTCCTTAGGATACCTACTAATCAGGACACTTAAACATCTTCGGATATCGGCCTGTGAAAATATGGAGAAAATAGTCGAAAGGTTCCATGATAACATTCTTCTTGAAGAACTTCAATTATGGACATGTCCAAATCTTAAATCCTTCCCGGAGGGTCTATGCCACCTTACCAATCTTCTATCACTATCGATCATAAACTGTGGAAGTTTTGTTTCCTTCCCAACAGAAGTGTTTCCAAGAAGCGCCTTCAACTTGAGGATGATAGAGATTTCCAACTGCCCTAATTTGGAGGGCATCCCCAAAGGCTTGCAGAACCTGAACTCTCTTCAGGACTTTCATGGTTTGGCGTCATTTCTAGATGAGGGTTTTCCCACCAATCTAACAAGACTAGTAGTTTGGAAAGTCAAGAATTGCAAGGCGCTATTGGAGTTGGGATTGCACAGACTCACCTCTCTTAGAGAGTTGTATATCAGTGGTGAAGATCCAGATTTGGTGTCCTTTCCACCCGAGGACAAGATGACACCACCCCTGCCCAAATCTCTCATTCAAATCACAATTGCCGACTTCCCAAATCTTACTAAACTCAGCGAAGGCTTTCAATTCCTCACCTCTCTTGAATATCTTGAAGTATCTTCTTGTCCTAAACTAGCATCAATTCCAGAAGGGGGTTTGCCTCTTTCACTTAATCGACTTTATGTCATTCAATGTCCTCTGCTACAAGAGAGGTGCAAACCAATGAAAGGTAGATACTGGCCCTCTATAGCCCACATCCCTTGGATAACTAATGGAGGCATGGACATCCGGTTCTACTCCCTCCAAGAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1380

Amino Acids

157.29

Weight (kDa)

6.58

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 96 1.6e-24 Rx N-terminal domain
NB-ARC PF00931 173 - 343 1.6e-36 NB-ARC domain
WHD_DRP PF23559 429 - 497 4.9e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 565 - 658 2.9e-13 Leucine-rich repeat region
LRR_14 PF23598 579 - 849 6.6e-20 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 692 - 813 1.4e-31 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2709
AasI GACNNNNNNGTC 1 cut(s) 880
AatII GACGTC 1 cut(s) 3320
AccB7I CCANNNNNTGG 1 cut(s) 2396
AccBSI CCGCTC 1 cut(s) 2521
AciI CCGC 6 cut(s) 271, 1050, 1098, 1648, 2147, 2521
AclWI GGATC 3 cut(s) 1318, 2746, 3791
AcoI YGGCCR 2 cut(s) 2002, 2052
AcuI CTGAAG 5 cut(s) 180, 1236, 2888, 2987, 3626
AcvI CACGTG 1 cut(s) 932
AcyI GRCGYC 3 cut(s) 26, 3317, 3662
AfaI GTAC 5 cut(s) 315, 1870, 2202, 2820, 3205
AfiI CCNNNNNNNGG 9 cut(s) 503, 605, 1336, 2396, 2536, 2830, 3601, 3980, 4055
AflII CTTAAG 2 cut(s) 35, 674
AflIII ACRYGT 3 cut(s) 929, 2247, 3440
AhdI GACNNNNNGTC 2 cut(s) 3276, 4010
AhlI ACTAGT 3 cut(s) 446, 3275, 3703
AjiI CACGTC 1 cut(s) 747
AjnI CCWGG 6 cut(s) 216, 598, 904, 1681, 1999, 2440
AleI CACNNNNGTG 1 cut(s) 2663
AloI GAACNNNNNNTCC 2 cut(s) 4103, 4135
Alw21I GWGCWC 1 cut(s) 904
Alw26I GTCTC 3 cut(s) 2224, 3263, 3284
Alw44I GTGCAC 1 cut(s) 900
AlwI GGATC 3 cut(s) 1318, 2746, 3791
AlwNI CAGNNNCTG 1 cut(s) 3631
Ama87I CYCGRG 2 cut(s) 3281, 3821
AoxI GGCC 7 cut(s) 464, 2002, 2052, 2163, 2787, 3366, 4067
ApaLI GTGCAC 1 cut(s) 900
ApeKI GCWGC 8 cut(s) 43, 131, 148, 286, 1115, 2610, 2683, 2765
ArsI GACNNNNNNTTYG 2 cut(s) 3693, 3725
Asp700I GAANNNNTTC 6 cut(s) 665, 770, 1013, 3426, 3546, 3909
AspLEI GCGC 2 cut(s) 3560, 3736
AspS9I GGNCC 5 cut(s) 1052, 2087, 2527, 2788, 4068
AsuC2I CCSGG 2 cut(s) 2105, 3464
AsuHPI GGTGA 5 cut(s) 2365, 2761, 3754, 3803, 3913
AsuII TTCGAA 2 cut(s) 1620, 1973
AvaI CYCGRG 2 cut(s) 3281, 3821
AvaII GGWCC 3 cut(s) 1052, 2087, 2527
AxyI CCTNAGG 2 cut(s) 1745, 3322
BaeGI GKGCMC 1 cut(s) 904
BalI TGGCCA 1 cut(s) 2004
BarI GAAGNNNNNNTAC 2 cut(s) 3196, 3228
BbrPI CACGTG 1 cut(s) 932
BbsI GAAGAC 1 cut(s) 3219
Bbv12I GWGCWC 1 cut(s) 904
BbvI GCAGC 8 cut(s) 30, 118, 135, 298, 1102, 2597, 2695, 2752
BccI CCATC 7 cut(s) 756, 782, 1330, 1598, 2155, 2518, 2609
BciT130I CCWGG 6 cut(s) 218, 600, 906, 1683, 2001, 2442
BciVI GTATCC 1 cut(s) 3320
BclI TGATCA 2 cut(s) 379, 1630
BcnI CCSGG 2 cut(s) 2105, 3464
BcoDI GTCTC 3 cut(s) 2224, 3263, 3284
BcuI ACTAGT 3 cut(s) 446, 3275, 3703
BfmI CTRYAG 3 cut(s) 878, 2621, 4074
BfoI RGCGCY 2 cut(s) 3561, 3737
BfrI CTTAAG 2 cut(s) 35, 674
BfuI GTATCC 1 cut(s) 3320
BglII AGATCT 4 cut(s) 15, 1556, 1780, 2863
BisI GCNGC 8 cut(s) 44, 132, 149, 287, 1116, 2611, 2684, 2766
BlsI GCNGC 8 cut(s) 45, 133, 150, 288, 1117, 2612, 2685, 2767
BmcAI AGTACT 2 cut(s) 1870, 3205
Bme1390I CCNGG 8 cut(s) 218, 600, 906, 1683, 2001, 2105, 2442, 3464
Bme18I GGWCC 3 cut(s) 1052, 2087, 2527
BmeRI GACNNNNNGTC 2 cut(s) 3276, 4010
BmeT110I CYCGRG 2 cut(s) 3281, 3821
BmgBI CACGTC 1 cut(s) 747
BmgT120I GGNCC 5 cut(s) 1052, 2087, 2527, 2788, 4068
BmiI GGNNCC 2 cut(s) 2089, 3401
BmrFI CCNGG 8 cut(s) 218, 600, 906, 1683, 2001, 2105, 2442, 3464
BmsI GCATC 9 cut(s) 145, 925, 2107, 2125, 2134, 2143, 3249, 3618, 3974
BpiI GAAGAC 1 cut(s) 3219
BplI GAGNNNNNCTC 2 cut(s) 527, 559
BpmI CTGGAG 1 cut(s) 2463
Bpu10I CCTNAGC 1 cut(s) 461
Bpu14I TTCGAA 2 cut(s) 1620, 1973
BpuEI CTTGAG 3 cut(s) 529, 814, 3589
BpuMI CCSGG 2 cut(s) 2105, 3464
Bsa29I ATCGAT 2 cut(s) 1793, 3503
BsaAI YACGTR 1 cut(s) 932
BsaHI GRCGYC 3 cut(s) 26, 3317, 3662
BsaJI CCNNGG 3 cut(s) 2104, 3822, 4088
BsaWI WCCGGW 1 cut(s) 4116
BsaXI ACNNNNNCTCC 2 cut(s) 1793, 1823
Bsc4I CCNNNNNNNGG 9 cut(s) 503, 605, 1336, 2396, 2536, 2830, 3601, 3980, 4055
Bse1I ACTGG 3 cut(s) 1866, 2928, 4070
Bse21I CCTNAGG 2 cut(s) 1745, 3322
Bse3DI GCAATG 2 cut(s) 1456, 2111
BseBI CCWGG 6 cut(s) 218, 600, 906, 1683, 2001, 2442
BseCI ATCGAT 2 cut(s) 1793, 3503
BseDI CCNNGG 3 cut(s) 2104, 3822, 4088
BseLI CCNNNNNNNGG 9 cut(s) 503, 605, 1336, 2396, 2536, 2830, 3601, 3980, 4055
BseMI GCAATG 2 cut(s) 1456, 2111
BseMII CTCAG 6 cut(s) 249, 638, 2628, 2783, 3221, 3913
BseNI ACTGG 3 cut(s) 1866, 2928, 4070
BseSI GKGCMC 1 cut(s) 904
BseXI GCAGC 8 cut(s) 30, 118, 135, 298, 1102, 2597, 2695, 2752
BshFI GGCC 7 cut(s) 466, 2004, 2054, 2165, 2789, 3368, 4069
BshVI ATCGAT 2 cut(s) 1793, 3503
BsiHKAI GWGCWC 1 cut(s) 904
BsiHKCI CYCGRG 2 cut(s) 3281, 3821
BsiSI CCGG 3 cut(s) 2104, 3464, 4117
BslFI GGGAC 7 cut(s) 448, 733, 868, 2073, 2122, 2580, 3291
BslI CCNNNNNNNGG 9 cut(s) 503, 605, 1336, 2396, 2536, 2830, 3601, 3980, 4055
BsmAI GTCTC 3 cut(s) 2224, 3263, 3284
BsmFI GGGAC 7 cut(s) 448, 733, 868, 2073, 2122, 2580, 3291
BsmI GAATGC 1 cut(s) 2001
BsnI GGCC 7 cut(s) 466, 2004, 2054, 2165, 2789, 3368, 4069
BsoBI CYCGRG 2 cut(s) 3281, 3821
Bsp119I TTCGAA 2 cut(s) 1620, 1973
Bsp1286I GDGCHC 1 cut(s) 904
BspACI CCGC 6 cut(s) 271, 1050, 1098, 1648, 2147, 2521
BspANI GGCC 7 cut(s) 466, 2004, 2054, 2165, 2789, 3368, 4069
BspCNI CTCAG 6 cut(s) 250, 637, 2629, 2782, 3220, 3912
BspDI ATCGAT 2 cut(s) 1793, 3503
BspHI TCATGA 1 cut(s) 1717
BspLI GGNNCC 2 cut(s) 2089, 3401
BspMAI CTGCAG 1 cut(s) 2625
BspPI GGATC 3 cut(s) 1318, 2746, 3791
BspT104I TTCGAA 2 cut(s) 1620, 1973
BspTI CTTAAG 2 cut(s) 35, 674
BsrBI CCGCTC 1 cut(s) 2521
BsrDI GCAATG 2 cut(s) 1456, 2111
BsrI ACTGG 3 cut(s) 1866, 2928, 4070
BssECI CCNNGG 3 cut(s) 2104, 3822, 4088
BssNI GRCGYC 3 cut(s) 26, 3317, 3662
BssT1I CCWWGG 1 cut(s) 4088
Bst2UI CCWGG 6 cut(s) 218, 600, 906, 1683, 2001, 2442
Bst6I CTCTTC 4 cut(s) 156, 502, 3042, 3645
BstACI GRCGYC 3 cut(s) 26, 3317, 3662
BstAFI CTTAAG 2 cut(s) 35, 674
BstAPI GCANNNNNTGC 1 cut(s) 944
BstBAI YACGTR 1 cut(s) 932
BstBI TTCGAA 2 cut(s) 1620, 1973
BstC8I GCNNGC 4 cut(s) 129, 2381, 2486, 3623
BstEII GGTNACC 1 cut(s) 2631
BstENI CCTNNNNNAGG 1 cut(s) 2534
BstH2I RGCGCY 2 cut(s) 3561, 3737
BstHHI GCGC 2 cut(s) 3560, 3736
BstMAI GTCTC 3 cut(s) 2224, 3263, 3284
BstMWI GCNNNNNNNGC 6 cut(s) 52, 140, 944, 1735, 2153, 2162
BstNI CCWGG 6 cut(s) 218, 600, 906, 1683, 2001, 2442
BstNSI RCATGY 4 cut(s) 127, 1028, 2251, 3444
BstPI GGTNACC 1 cut(s) 2631
BstSCI CCNGG 8 cut(s) 216, 598, 904, 1681, 1999, 2103, 2440, 3462
BstSFI CTRYAG 3 cut(s) 878, 2621, 4074
BstSLI GKGCMC 1 cut(s) 904
BstV1I GCAGC 8 cut(s) 30, 118, 135, 298, 1102, 2597, 2695, 2752
BstV2I GAAGAC 1 cut(s) 3219
BstX2I RGATCY 5 cut(s) 15, 1556, 1780, 2863, 3796
BstYI RGATCY 5 cut(s) 15, 1556, 1780, 2863, 3796
Bsu15I ATCGAT 2 cut(s) 1793, 3503
Bsu36I CCTNAGG 2 cut(s) 1745, 3322
BsuI GTATCC 1 cut(s) 3320
BsuRI GGCC 7 cut(s) 466, 2004, 2054, 2165, 2789, 3368, 4069
BsuTUI ATCGAT 2 cut(s) 1793, 3503
BtrI CACGTC 1 cut(s) 747
BtsI GCAGTG 3 cut(s) 12, 192, 1676
BtsIMutI CAGTG 8 cut(s) 12, 192, 255, 649, 696, 754, 1676, 3793
Cac8I GCNNGC 4 cut(s) 129, 2381, 2486, 3623
CaiI CAGNNNCTG 1 cut(s) 3631
CciI TCATGA 1 cut(s) 1717
CfoI GCGC 2 cut(s) 3560, 3736
Cfr13I GGNCC 5 cut(s) 1052, 2087, 2527, 2788, 4068
ClaI ATCGAT 2 cut(s) 1793, 3503
CseI GACGC 4 cut(s) 15, 1538, 1946, 3651
Csp6I GTAC 5 cut(s) 314, 1869, 2201, 2819, 3204
CspCI CAANNNNNGTGG 4 cut(s) 1081, 1116, 4070, 4105
CviQI GTAC 5 cut(s) 314, 1869, 2201, 2819, 3204
DraI TTTAAA 1 cut(s) 3175
DrdI GACNNNNNNGTC 1 cut(s) 880
DriI GACNNNNNGTC 2 cut(s) 3276, 4010
DseDI GACNNNNNNGTC 1 cut(s) 880
EaeI YGGCCR 2 cut(s) 2002, 2052
Eam1104I CTCTTC 4 cut(s) 156, 502, 3042, 3645
Eam1105I GACNNNNNGTC 2 cut(s) 3276, 4010
EarI CTCTTC 4 cut(s) 156, 502, 3042, 3645
Eco130I CCWWGG 1 cut(s) 4088
Eco32I GATATC 1 cut(s) 3363
Eco47I GGWCC 3 cut(s) 1052, 2087, 2527
Eco57I CTGAAG 5 cut(s) 180, 1236, 2888, 2987, 3626
Eco72I CACGTG 1 cut(s) 932
Eco81I CCTNAGG 2 cut(s) 1745, 3322
Eco88I CYCGRG 2 cut(s) 3281, 3821
Eco91I GGTNACC 1 cut(s) 2631
EcoNI CCTNNNNNAGG 1 cut(s) 2534
EcoO109I RGGNCCY 1 cut(s) 2788
EcoO65I GGTNACC 1 cut(s) 2631
EcoRI GAATTC 1 cut(s) 770
EcoRII CCWGG 6 cut(s) 216, 598, 904, 1681, 1999, 2440
EcoRV GATATC 1 cut(s) 3363
EcoT14I CCWWGG 1 cut(s) 4088
EcoT22I ATGCAT 1 cut(s) 1470
ErhI CCWWGG 1 cut(s) 4088
FalI AAGNNNNNCTT 4 cut(s) 399, 431, 717, 749
FaqI GGGAC 7 cut(s) 448, 733, 868, 2073, 2122, 2580, 3291
FbaI TGATCA 2 cut(s) 379, 1630
Fnu4HI GCNGC 8 cut(s) 44, 132, 149, 287, 1116, 2611, 2684, 2766
Fsp4HI GCNGC 8 cut(s) 44, 132, 149, 287, 1116, 2611, 2684, 2766
GlaI GCGC 2 cut(s) 3559, 3735
GluI GCNGC 8 cut(s) 44, 132, 149, 287, 1116, 2611, 2684, 2766
GsuI CTGGAG 1 cut(s) 2463
HaeII RGCGCY 2 cut(s) 3561, 3737
HaeIII GGCC 7 cut(s) 466, 2004, 2054, 2165, 2789, 3368, 4069
HapII CCGG 3 cut(s) 2104, 3464, 4117
HgaI GACGC 4 cut(s) 15, 1538, 1946, 3651
HhaI GCGC 2 cut(s) 3560, 3736
Hin1I GRCGYC 3 cut(s) 26, 3317, 3662
Hin6I GCGC 2 cut(s) 3558, 3734
HinP1I GCGC 2 cut(s) 3558, 3734
HincII GTYRAC 1 cut(s) 2850
HindII GTYRAC 1 cut(s) 2850
HindIII AAGCTT 3 cut(s) 368, 413, 2186
HpaII CCGG 3 cut(s) 2104, 3464, 4117
HphI GGTGA 5 cut(s) 2365, 2761, 3754, 3803, 3913
Hpy166II GTNNAC 5 cut(s) 517, 902, 973, 2658, 2850
Hpy8I GTNNAC 5 cut(s) 517, 902, 973, 2658, 2850
HpyCH4IV ACGT 3 cut(s) 746, 931, 3317
HpyF10VI GCNNNNNNNGC 6 cut(s) 52, 140, 944, 1735, 2153, 2162
HpySE526I ACGT 3 cut(s) 746, 931, 3317
Hsp92I GRCGYC 3 cut(s) 26, 3317, 3662
HspAI GCGC 2 cut(s) 3558, 3734
Ksp22I TGATCA 2 cut(s) 379, 1630
LmnI GCTCC 3 cut(s) 140, 283, 1505
Lsp1109I GCAGC 8 cut(s) 30, 118, 135, 298, 1102, 2597, 2695, 2752
LweI GCATC 9 cut(s) 145, 925, 2107, 2125, 2134, 2143, 3249, 3618, 3974
MaeII ACGT 3 cut(s) 746, 931, 3317
MaeIII GTNAC 7 cut(s) 384, 718, 742, 854, 1208, 1541, 2631
MbiI CCGCTC 1 cut(s) 2521
MfeI CAATTG 4 cut(s) 1376, 1577, 2402, 3870
MflI RGATCY 5 cut(s) 15, 1556, 1780, 2863, 3796
MhlI GDGCHC 1 cut(s) 904
MlsI TGGCCA 1 cut(s) 2004
MluNI TGGCCA 1 cut(s) 2004
MlyI GAGTC 4 cut(s) 1201, 1696, 2786, 3753
MmeI TCCRAC 5 cut(s) 484, 675, 986, 1968, 3612
Mox20I TGGCCA 1 cut(s) 2004
Mph1103I ATGCAT 1 cut(s) 1470
MroXI GAANNNNTTC 6 cut(s) 665, 770, 1013, 3426, 3546, 3909
MscI TGGCCA 1 cut(s) 2004
MslI CAYNNNNRTG 5 cut(s) 260, 897, 2663, 2676, 2760
Msp20I TGGCCA 1 cut(s) 2004
MspCI CTTAAG 2 cut(s) 35, 674
MspI CCGG 3 cut(s) 2104, 3464, 4117
MspR9I CCNGG 8 cut(s) 218, 600, 906, 1683, 2001, 2105, 2442, 3464
MunI CAATTG 4 cut(s) 1376, 1577, 2402, 3870
Mva1269I GAATGC 1 cut(s) 2001
MvaI CCWGG 6 cut(s) 218, 600, 906, 1683, 2001, 2442
MwoI GCNNNNNNNGC 6 cut(s) 52, 140, 944, 1735, 2153, 2162
NciI CCSGG 2 cut(s) 2105, 3464
NlaIV GGNNCC 2 cut(s) 2089, 3401
NmuCI GTSAC 2 cut(s) 742, 854
NsiI ATGCAT 1 cut(s) 1470
NspI RCATGY 4 cut(s) 127, 1028, 2251, 3444
NspV TTCGAA 2 cut(s) 1620, 1973
OliI CACNNNNGTG 1 cut(s) 2663
PaeR7I CTCGAG 1 cut(s) 3281
PagI TCATGA 1 cut(s) 1717
PciI ACATGT 2 cut(s) 2247, 3440
PctI GAATGC 1 cut(s) 2001
PdmI GAANNNNTTC 6 cut(s) 665, 770, 1013, 3426, 3546, 3909
PfeI GAWTC 7 cut(s) 737, 1773, 1859, 1942, 2066, 2327, 2668
PflMI CCANNNNNTGG 1 cut(s) 2396
PfoI TCCNGGA 1 cut(s) 3462
PkrI GCNGC 8 cut(s) 45, 133, 150, 288, 1117, 2612, 2685, 2767
PleI GAGTC 4 cut(s) 1200, 1696, 2785, 3753
PmaCI CACGTG 1 cut(s) 932
PmlI CACGTG 1 cut(s) 932
PpsI GAGTC 4 cut(s) 1200, 1696, 2785, 3753
Ppu21I YACGTR 1 cut(s) 932
PscI ACATGT 2 cut(s) 2247, 3440
PsiI TTATAA 1 cut(s) 2709
Psp6I CCWGG 6 cut(s) 216, 598, 904, 1681, 1999, 2440
PspCI CACGTG 1 cut(s) 932
PspEI GGTNACC 1 cut(s) 2631
PspGI CCWGG 6 cut(s) 216, 598, 904, 1681, 1999, 2440
PspN4I GGNNCC 2 cut(s) 2089, 3401
PspPI GGNCC 5 cut(s) 1052, 2087, 2527, 2788, 4068
PsrI GAACNNNNNNTAC 2 cut(s) 1912, 1944
PstI CTGCAG 1 cut(s) 2625
PstNI CAGNNNCTG 1 cut(s) 3631
PsuI RGATCY 5 cut(s) 15, 1556, 1780, 2863, 3796
RsaI GTAC 5 cut(s) 315, 1870, 2202, 2820, 3205
RsaNI GTAC 5 cut(s) 314, 1869, 2201, 2819, 3204
RseI CAYNNNNRTG 5 cut(s) 260, 897, 2663, 2676, 2760
SatI GCNGC 8 cut(s) 44, 132, 149, 287, 1116, 2611, 2684, 2766
Sau96I GGNCC 5 cut(s) 1052, 2087, 2527, 2788, 4068
ScaI AGTACT 2 cut(s) 1870, 3205
SchI GAGTC 4 cut(s) 1201, 1696, 2786, 3753
ScrFI CCNGG 8 cut(s) 218, 600, 906, 1683, 2001, 2105, 2442, 3464
SduI GDGCHC 1 cut(s) 904
SfaNI GCATC 9 cut(s) 145, 925, 2107, 2125, 2134, 2143, 3249, 3618, 3974
SfcI CTRYAG 3 cut(s) 878, 2621, 4074
Sfr274I CTCGAG 1 cut(s) 3281
SfuI TTCGAA 2 cut(s) 1620, 1973
SinI GGWCC 3 cut(s) 1052, 2087, 2527
SlaI CTCGAG 1 cut(s) 3281
SmiMI CAYNNNNRTG 5 cut(s) 260, 897, 2663, 2676, 2760
SmlI CTYRAG 6 cut(s) 35, 544, 674, 793, 3281, 3568
SmoI CTYRAG 6 cut(s) 35, 544, 674, 793, 3281, 3568
SpeI ACTAGT 3 cut(s) 446, 3275, 3703
SsiI CCGC 6 cut(s) 271, 1050, 1098, 1648, 2147, 2521
StyD4I CCNGG 8 cut(s) 216, 598, 904, 1681, 1999, 2103, 2440, 3462
StyI CCWWGG 1 cut(s) 4088
TaiI ACGT 3 cut(s) 749, 934, 3320
TaqII GACCGA 1 cut(s) 2075
TatI WGTACW 2 cut(s) 1868, 3203
TfiI GAWTC 7 cut(s) 737, 1773, 1859, 1942, 2066, 2327, 2668
TscAI CASTG 8 cut(s) 12, 192, 262, 649, 696, 761, 1683, 3793
TseFI GTSAC 2 cut(s) 742, 854
TseI GCWGC 8 cut(s) 43, 131, 148, 286, 1115, 2610, 2683, 2765
Tsp45I GTSAC 2 cut(s) 742, 854
TspGWI ACGGA 2 cut(s) 74, 418
TspRI CASTG 8 cut(s) 12, 192, 262, 649, 696, 761, 1683, 3793
Van91I CCANNNNNTGG 1 cut(s) 2396
Vha464I CTTAAG 2 cut(s) 35, 674
VneI GTGCAC 1 cut(s) 900
VpaK11BI GGWCC 3 cut(s) 1052, 2087, 2527
XagI CCTNNNNNAGG 1 cut(s) 2534
XbaI TCTAGA 2 cut(s) 1418, 3670
XceI RCATGY 4 cut(s) 127, 1028, 2251, 3444
XhoI CTCGAG 1 cut(s) 3281
XmnI GAANNNNTTC 6 cut(s) 665, 770, 1013, 3426, 3546, 3909
ZraI GACGTC 1 cut(s) 3318
ZrmI AGTACT 2 cut(s) 1870, 3205
Zsp2I ATGCAT 1 cut(s) 1470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.