RchiOBHm_Chr1g0320131

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000070
Physical Location & Seq
Reverse (-)
7800320 .. 7806351
6032 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55030

Sequence Viewer

Length: 4020 bp
ATGGCAGTGGGAGAGATCTTTCTTGCGGCATTCCTTCAGTTGCTGCTGGACAGGTTAACGCCTCGGGAGATCCTGGGCTACTTTGGAAGCTTGGGAGGAGCTGGCAAAAAGCTGCAGAAATGGAGAGAGACACTTTCCGCAGTTGCAGCGGTGCTTTCCGATGCGGAGGAGAAGCAGCTGACGAGCAAGGCAGTGGATCTGTGGCTGAATGACCTGAAACACTTGGCTTATGATCTGGACGATCTACTCAACACCTTTTCTAATGAGATGTTAGAACGCGAGCAGCTCAAGCTTCACCAAACTGGCACAAGCAAGGTACGAGGCTTCTTTACCAAAGTTCCTCACAAAGTTAAGTTCAATTTCAATATGAACTCCGAAATAGACGACATTGCTAATCGCTTGCAAGACATATTTGACCGGAAAGAGAAACTCGGTTTGAAATATATTGAGCATACCTCTACTTCAACATCGTCATCGCACAGGACACCAAGTTCATATGTGTTGGATGGACCTGTGGTTGGGAGAGATGAAGACACAAGAAAGATTGTGGAATTGTTGTCCAGAGGTGTTGATCCTTCTTCTCCCACAAATTATCAAGTCGTTGCCATTGTTGGTATGGGTGGACTCGGCAAGACAACGCTTGCGGGACGAGTCTTCAATGATGTAGCTACAGTGGAACAGTTTAATCTCAAGATCTGGGTTTCAGTATCCGATAACTTTGATCTTCAAACGGTGACGAGAGCTATTTTTAAGGAAGTTACATCTCGGCCTTGTGATATGGATGAGTTCAGTCGACTTCAAGATAATCTGAGCAAGAAGATCGATGGTAAAAGGTTTTTGATTGTTTTAGATGATGTCTGGAGCACGTGTGATTATCATTCATGGACAAAACTGCTAGCTCCCTTCTGTGGGGGAGCTAAGGGAAGTAAGATAATGGTGACAACACGTGATAGCCAAGTTGCAACATCGATGGGAGCCCCAGCCGCTGAACATGTTAGCAATGACAGGCCACCGAATTATGACTTGCTTAAGAAGAAAATTGTTACGAATTGCAATGGATTGCCATTGGCTGCAAAAACTCTTGGTGGTGTTTTACGTTGTGAAGAAACTGGCAAGTGGGAGGAAATACTGAATGATAAATTATGGAGTATATCAGATGGGAGTAATATACTCCCAGTACTCAGATTGAGTTATCATGATCTCCCTTCACCTTTGAAGAGGTGCTTTGCCTATTGCTCAATACTTCCCAATGACTATGAATTTGGGAAAACCCAATTAATCCTTCTGTGGATGGCAGAGGGTTTTCTTGAGCAACCAGGAGGAAGTAAAGAGATGGAATATATCGGTGACGAATATTTTGGGGAGCTATTGTCTCGGTCATTCTTTCAAAAATCAGCCAAAAACGATTCATGCTATGTAATGGATGGCGTTGTTGGTGATTTGGCACGATGGGCTGCTGCAGACATATTTTGCAGATTGGAGGATAAACCGCATGGTAGATGTTCTGCAAATACTCGTCATCTGAGTTACATTTCTGCTAAGTTTGATGGGAAACAAAGATTTGAGGAGACATTGCCTAAGGCACAACACTTGCGGACTTTCCTACCTCTTCCAGTTTCCAGTGGTTATGAGAATTATCTAACTCATTATGCTACTTCTGATTTATTGCCACAATTGAAGTACTTGCGGGTGCTCTCCTTCAATGGTTATAAACTGACCGAGCTGCCAGGTTCTGTAGGCAAGTTGAGACATCTACGGTACCTTGATCTTTCTCACACATTGATAATGACTTTGCCTGAGTCAACATGCACACTTTACAACTTACAGACGTTAATATTAGAGAATTGTTCAAAATTGAAGACACTACCTTCAAACATGAGCAATTTATCTAATTTACGCCATCTCAACAATTCAAACATGCCTTCATTGGATGAAATGCCTCCCCACGTAAGTCAGTTGACTCATCTGCGAACTTTGCCTAATTTTGTGGTGGGGAAAGGTAGTGCATCAGGGATTGGAGAGATACAGTCATTGCATCTTCGTGGGACCTTGAGTGTTGGAAGATTGGAAAATGTGATTGATGTTGGGGATGCGAAGACGGCTCAAATAATGAACAAGGAAGGGCTGGAAATCTTGCAATTGGAATGGAGCGGCACAGGCGAGAAGGAGTTGGAGGTGCTGTGCAGTTTAGAACCTCAAAAAAAGCTTCTACATCTCACTATCAAAGGTTACAATGGTTTTGAATTTTCAAAATGGATTGGACATCCTTCATTCTCTGATATGATATCTGTGAAGTTAGAGAATTGTAAAAACTGTCGATTTTTGCCTCCTGTTGGCCAATTACCTTTGCTGAAAAAACTTTGGATACAAGGACTGGCCAATGTTAAAAGTGTGGGTGCTGAGTTTTATGGAGAGTGTAGCTTGCCTTTTCCAGTGTTGGAAGTCCTGACGTTCAAGGATATGCAAAATTGGAAGGAGTGGCTTCCTTGCAAAAGAGATGAAGAAATTCGAGTTTTCCCATGCATGGAAAAGCTTTCCGTCCTTAGTTGCCCCAAATTGAAAGGTTGGTTGCCCAAGAACATGGATTCATTATCAGAGCTGTATATTGATGAATGTGAAGAGTTAGTGGTTTCAATTGCCAATTATAAACATATACATAAGTTGACCATCAACGGTTGTAAAAGGGTGGTGCACAGAAGTGGAGTTAAGTTTGAGCTACTGGAGGATATGGAGCTTCATGGTATTTCAGAGTTCAGACTCGAAATAGACGGGTTCATCAGAGGATTGCCAAAGCTTCAAACTTTACGGATTACTGGTTGTGAAGAGTTGACATGTTTATGGGAGAATGAGGATAGATTGCTGCAGCGTCGAATTTCTAATATTATTGGAGGCAACATATCTGACTCCCCCTCTCACTTTATTCAAGAGCTAAGAGCACTCAAGGAACTTGTGCTAACTGGATGCTCAAATCTAATTTCTTTTCCAGAAGCTAGTTGGCCACCTTGTCTTGAAAATGTAAAGATGGAGTCGTGTAATTCTTTGACATATTTCGTAAGGTATCAAGTACCGCCAAGCATAAGAAGAATAGAGATAGAAAAATGTCAAAATTTGAAACTACTAGTGAAGGATGCTGGGGAGCTAGAGGGTTGTATGGAATGGTTGGAGATAAAAGAGTGTGCATCTTTGACATCCTTATCAGGCAAAGGAGGCCGACTACCCAGAACCCTCAAATTTCTTAGAATAAGTGACTGTGACCGATTGGAGTCAATAATCGAAACATTCCACGAGGACACTTGTCTTGAAGCTCTTACGATAGAGAGGTGTGCAAATCTCAAATCCTTACCAGAGGGGCTATGCCATCTCTCCGCTCTTCGCATTTTAATTATACGCAATTGTGGAAGTCTCGTTTCCTTCCCAAGAGGAGGGTTGCCATCCAACCTGATAGACTTTTGTATTGAAGATTGTGATCAATTGGAAGCCCTCCCCAGAGGAATGGACAACCTCAGCTCTCTTCAGACTTTGATGTTGAGATACTATGGAGGTTTCCCACCCAACCTAATTACTCTTCGAATTGGCAATTGCAAAAGCTGCAAGCCTCTCTCAGAGTGGGGGCTTCACCACTTGGACAGAGTCACCTCTCTTAAACACTTGTGGATCAATGGTGTAGATCCAGATCTGGTGTCCTTTCCGCCAAAGGAGGTGCTGCTCCCTAAATCTCTTATTGAACTCACCATTCGAGGCCTCCCAAATCTGAAGCGCCTGTCATCATCTTTTCAATCACTCTCCTCTCTTGAATCCCTTAACATTTGGGATTGCCCAAAGCTAGCATCTATTATTCCAGAAAAAGAGGATCATCTGCCTCTTTCACTTACACAGCTTCGCATCTATCATCACTGTCCGCTGCTCACAAAGAAATACCAACCAGGTAAAGGACGACACTGGCCCAAAGAAATAGCCCACATCCCTTACGTTTATTTTGGAGACTACGGTGACGAAGCCGAAGCCGACCGCTCGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1339

Amino Acids

151.03

Weight (kDa)

6.55

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 99 4.8e-17 Rx N-terminal domain
NB-ARC PF00931 180 - 332 1.7e-33 NB-ARC domain
WHD_DRP PF23559 414 - 482 1.1e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 556 - 822 1.4e-20 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 672 - 791 6.3e-30 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1710, 2646
Acc65I GGTACC 1 cut(s) 1758
AccB1I GGYRCC 1 cut(s) 1758
AccBSI CCGCTC 3 cut(s) 2151, 3368, 4011
AccI GTMKAC 1 cut(s) 793
AccII CGCG 1 cut(s) 279
AclWI GGATC 6 cut(s) 64, 204, 566, 3662, 3662, 3858
AcoI YGGCCR 3 cut(s) 2335, 2376, 2996
AcsI RAATTY 6 cut(s) 1259, 2243, 2505, 2871, 3106, 3230
AcuI CTGAAG 3 cut(s) 20, 3497, 3773
AcvI CACGTG 2 cut(s) 867, 947
AfaI GTAC 5 cut(s) 318, 1179, 1682, 1760, 3066
AfiI CCNNNNNNNGG 7 cut(s) 518, 908, 909, 2333, 2524, 3422, 3929
AflII CTTAAG 1 cut(s) 1028
AflIII ACRYGT 4 cut(s) 866, 944, 991, 2831
AhlI ACTAGT 1 cut(s) 3118
AjnI CCWGG 4 cut(s) 72, 1315, 1726, 3922
AjuI GAANNNNNNNTTGG 2 cut(s) 1266, 1298
AleI CACNNNNGTG 1 cut(s) 2697
AloI GAACNNNNNNTCC 2 cut(s) 475, 507
Alw21I GWGCWC 4 cut(s) 866, 1695, 2694, 2938
Alw26I GTCTC 6 cut(s) 122, 1377, 1562, 1741, 3407, 3975
Alw44I GTGCAC 1 cut(s) 2690
AlwI GGATC 6 cut(s) 64, 204, 566, 3662, 3662, 3858
AlwNI CAGNNNCTG 3 cut(s) 43, 986, 1733
Ama87I CYCGRG 1 cut(s) 63
AoxI GGCC 8 cut(s) 767, 1007, 2335, 2376, 2996, 3208, 3739, 3941
ApaLI GTGCAC 1 cut(s) 2690
ApoI RAATTY 6 cut(s) 1259, 2243, 2505, 2871, 3106, 3230
ArsI GACNNNNNNTTYG 4 cut(s) 1244, 1276, 1340, 1372
AseI ATTAAT 1 cut(s) 1277
Asp700I GAANNNNTTC 1 cut(s) 2505
Asp718I GGTACC 1 cut(s) 1758
AspLEI GCGC 1 cut(s) 3759
AspS9I GGNCC 3 cut(s) 509, 2046, 3942
AsuII TTCGAA 1 cut(s) 3568
AsuNHI GCTAGC 2 cut(s) 895, 3823
AvaI CYCGRG 1 cut(s) 63
AvaII GGWCC 2 cut(s) 509, 2046
AxyI CCTNAGG 1 cut(s) 1578
BaeGI GKGCMC 1 cut(s) 2694
BalI TGGCCA 3 cut(s) 2337, 2378, 2998
BanI GGYRCC 1 cut(s) 1758
BanII GRGCYC 1 cut(s) 979
BauI CACGAG 1 cut(s) 3284
BbrPI CACGTG 2 cut(s) 867, 947
BbsI GAAGAC 4 cut(s) 537, 646, 1865, 2102
Bbv12I GWGCWC 4 cut(s) 866, 1695, 2694, 2938
BbvCI CCTCAGC 1 cut(s) 3503
BceAI ACGGC 1 cut(s) 2115
BcgI CGANNNNNNTGC 2 cut(s) 802, 836
BciT130I CCWGG 4 cut(s) 74, 1317, 1728, 3924
BciVI GTATCC 2 cut(s) 718, 2358
BclI TGATCA 1 cut(s) 3466
BcoDI GTCTC 6 cut(s) 122, 1377, 1562, 1741, 3407, 3975
BcuI ACTAGT 1 cut(s) 3118
BfaI CTAG 5 cut(s) 896, 2991, 3119, 3140, 3824
BfmI CTRYAG 5 cut(s) 113, 669, 1458, 1734, 2861
BfoI RGCGCY 1 cut(s) 3760
BfrI CTTAAG 1 cut(s) 1028
BfuI GTATCC 2 cut(s) 718, 2358
BglII AGATCT 3 cut(s) 15, 693, 3673
BmcAI AGTACT 2 cut(s) 1179, 1682
Bme1390I CCNGG 4 cut(s) 74, 1317, 1728, 3924
Bme18I GGWCC 2 cut(s) 509, 2046
BmeT110I CYCGRG 1 cut(s) 63
BmgT120I GGNCC 3 cut(s) 509, 2046, 3942
BmiI GGNNCC 3 cut(s) 976, 1760, 2047
BmrFI CCNGG 4 cut(s) 74, 1317, 1728, 3924
BmrI ACTGGG 1 cut(s) 1169
BmsI GCATC 9 cut(s) 151, 2015, 2044, 2080, 2951, 3118, 3188, 3836, 3891
BmtI GCTAGC 2 cut(s) 899, 3827
BmuI ACTGGG 1 cut(s) 1169
BoxI GACNNNNGTC 1 cut(s) 3294
BpiI GAAGAC 4 cut(s) 537, 646, 1865, 2102
BplI GAGNNNNNCTC 2 cut(s) 440, 472
BpmI CTGGAG 2 cut(s) 880, 2741
Bpu10I CCTNAGC 2 cut(s) 918, 3503
Bpu14I TTCGAA 1 cut(s) 3568
BpuEI CTTGAG 5 cut(s) 272, 674, 1328, 2071, 2924
Bsa29I ATCGAT 2 cut(s) 822, 968
BsaAI YACGTR 3 cut(s) 867, 947, 1948
BsaBI GATNNNNATC 2 cut(s) 3465, 3672
BsaJI CCNNGG 2 cut(s) 62, 73
BsaWI WCCGGW 1 cut(s) 417
BsaXI ACNNNNNCTCC 6 cut(s) 853, 883, 1355, 1385, 2165, 2195
Bsc4I CCNNNNNNNGG 7 cut(s) 518, 908, 909, 2333, 2524, 3422, 3929
Bse21I CCTNAGG 1 cut(s) 1578
Bse3DI GCAATG 5 cut(s) 387, 1006, 1060, 1571, 2030
Bse8I GATNNNNATC 2 cut(s) 3465, 3672
BseBI CCWGG 4 cut(s) 74, 1317, 1728, 3924
BseCI ATCGAT 2 cut(s) 822, 968
BseDI CCNNGG 2 cut(s) 62, 73
BseJI GATNNNNATC 2 cut(s) 3465, 3672
BseLI CCNNNNNNNGG 7 cut(s) 518, 908, 909, 2333, 2524, 3422, 3929
BseMI GCAATG 5 cut(s) 387, 1006, 1060, 1571, 2030
BseMII CTCAG 7 cut(s) 800, 1195, 1514, 1788, 2391, 3517, 3615
BseRI GAGGAG 5 cut(s) 111, 182, 1580, 3435, 3775
BseSI GKGCMC 1 cut(s) 2694
BseYI CCCAGC 2 cut(s) 979, 3131
BsgI GTGCAG 1 cut(s) 2203
Bsh1236I CGCG 1 cut(s) 279
Bsh1285I CGRYCG 1 cut(s) 4009
BshFI GGCC 8 cut(s) 769, 1009, 2337, 2378, 2998, 3210, 3741, 3943
BshNI GGYRCC 1 cut(s) 1758
BshVI ATCGAT 2 cut(s) 822, 968
BsiEI CGRYCG 1 cut(s) 4009
BsiHKAI GWGCWC 4 cut(s) 866, 1695, 2694, 2938
BsiHKCI CYCGRG 1 cut(s) 63
BsiSI CCGG 1 cut(s) 418
BslFI GGGAC 2 cut(s) 660, 2059
BslI CCNNNNNNNGG 7 cut(s) 518, 908, 909, 2333, 2524, 3422, 3929
BsmAI GTCTC 6 cut(s) 122, 1377, 1562, 1741, 3407, 3975
BsmFI GGGAC 2 cut(s) 660, 2059
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 8 cut(s) 769, 1009, 2337, 2378, 2998, 3210, 3741, 3943
BsoBI CYCGRG 1 cut(s) 63
Bsp119I TTCGAA 1 cut(s) 3568
Bsp1286I GDGCHC 5 cut(s) 866, 979, 1695, 2694, 2938
BspANI GGCC 8 cut(s) 769, 1009, 2337, 2378, 2998, 3210, 3741, 3943
BspCNI CTCAG 7 cut(s) 801, 1194, 1515, 1789, 2392, 3516, 3614
BspDI ATCGAT 2 cut(s) 822, 968
BspFNI CGCG 1 cut(s) 279
BspHI TCATGA 1 cut(s) 1195
BspLI GGNNCC 3 cut(s) 976, 1760, 2047
BspMAI CTGCAG 3 cut(s) 117, 1462, 2865
BspOI GCTAGC 2 cut(s) 899, 3827
BspPI GGATC 6 cut(s) 64, 204, 566, 3662, 3662, 3858
BspQI GCTCTTC 1 cut(s) 3375
BspT104I TTCGAA 1 cut(s) 3568
BspT107I GGYRCC 1 cut(s) 1758
BspTI CTTAAG 1 cut(s) 1028
BsrBI CCGCTC 3 cut(s) 2151, 3368, 4011
BsrDI GCAATG 5 cut(s) 387, 1006, 1060, 1571, 2030
BssECI CCNNGG 2 cut(s) 62, 73
BssSI CACGAG 1 cut(s) 3284
Bst2BI CACGAG 1 cut(s) 3284
Bst2UI CCWGG 4 cut(s) 74, 1317, 1728, 3924
Bst6I CTCTTC 7 cut(s) 1211, 1614, 2613, 2817, 3375, 3516, 3570
BstAFI CTTAAG 1 cut(s) 1028
BstAPI GCANNNNNTGC 1 cut(s) 3588
BstBAI YACGTR 3 cut(s) 867, 947, 1948
BstBI TTCGAA 1 cut(s) 3568
BstC8I GCNNGC 8 cut(s) 103, 281, 401, 642, 897, 2423, 3593, 3825
BstFNI CGCG 1 cut(s) 279
BstH2I RGCGCY 1 cut(s) 3760
BstHHI GCGC 1 cut(s) 3759
BstMAI GTCTC 6 cut(s) 122, 1377, 1562, 1741, 3407, 3975
BstMCI CGRYCG 1 cut(s) 4009
BstMWI GCNNNNNNNGC 9 cut(s) 146, 289, 983, 1451, 1975, 2099, 2157, 3207, 3588
BstNI CCWGG 4 cut(s) 74, 1317, 1728, 3924
BstNSI RCATGY 4 cut(s) 995, 1809, 1921, 2835
BstPAI GACNNNNGTC 1 cut(s) 3294
BstSCI CCNGG 4 cut(s) 72, 1315, 1726, 3922
BstSFI CTRYAG 5 cut(s) 113, 669, 1458, 1734, 2861
BstSLI GKGCMC 1 cut(s) 2694
BstUI CGCG 1 cut(s) 279
BstV2I GAAGAC 4 cut(s) 537, 646, 1865, 2102
BstX2I RGATCY 6 cut(s) 15, 69, 196, 693, 3667, 3673
BstXI CCANNNNNNTGG 2 cut(s) 2581, 3493
BstYI RGATCY 6 cut(s) 15, 69, 196, 693, 3667, 3673
Bsu15I ATCGAT 2 cut(s) 822, 968
Bsu36I CCTNAGG 1 cut(s) 1578
BsuI GTATCC 2 cut(s) 718, 2358
BsuRI GGCC 8 cut(s) 769, 1009, 2337, 2378, 2998, 3210, 3741, 3943
BsuTUI ATCGAT 2 cut(s) 822, 968
BtgZI GCGATG 1 cut(s) 459
BtsI GCAGTG 2 cut(s) 12, 198
BtsIMutI CAGTG 7 cut(s) 12, 198, 678, 1627, 2439, 3892, 3937
Cac8I GCNNGC 8 cut(s) 103, 281, 401, 642, 897, 2423, 3593, 3825
CaiI CAGNNNCTG 3 cut(s) 43, 986, 1733
CciI TCATGA 1 cut(s) 1195
CfoI GCGC 1 cut(s) 3759
Cfr13I GGNCC 3 cut(s) 509, 2046, 3942
ClaI ATCGAT 2 cut(s) 822, 968
CseI GACGC 1 cut(s) 2855
CsiI ACCWGGT 1 cut(s) 3922
Csp6I GTAC 5 cut(s) 317, 1178, 1681, 1759, 3065
CviQI GTAC 5 cut(s) 317, 1178, 1681, 1759, 3065
EaeI YGGCCR 3 cut(s) 2335, 2376, 2996
Eam1104I CTCTTC 7 cut(s) 1211, 1614, 2613, 2817, 3375, 3516, 3570
EarI CTCTTC 7 cut(s) 1211, 1614, 2613, 2817, 3375, 3516, 3570
EciI GGCGGA 1 cut(s) 3678
Eco147I AGGCCT 1 cut(s) 3741
Eco24I GRGCYC 1 cut(s) 979
Eco32I GATATC 1 cut(s) 2286
Eco47I GGWCC 2 cut(s) 509, 2046
Eco57I CTGAAG 3 cut(s) 20, 3497, 3773
Eco72I CACGTG 2 cut(s) 867, 947
Eco81I CCTNAGG 1 cut(s) 1578
Eco88I CYCGRG 1 cut(s) 63
EcoO109I RGGNCCY 1 cut(s) 2046
EcoRII CCWGG 4 cut(s) 72, 1315, 1726, 3922
EcoRV GATATC 1 cut(s) 2286
EcoT22I ATGCAT 1 cut(s) 2525
EcoT38I GRGCYC 1 cut(s) 979
FalI AAGNNNNNCTT 2 cut(s) 1208, 1240
FaqI GGGAC 2 cut(s) 660, 2059
FauI CCCGC 2 cut(s) 637, 1680
FauNDI CATATG 1 cut(s) 496
FbaI TGATCA 1 cut(s) 3466
FblI GTMKAC 1 cut(s) 793
FriOI GRGCYC 1 cut(s) 979
FspBI CTAG 5 cut(s) 896, 2991, 3119, 3140, 3824
GlaI GCGC 1 cut(s) 3758
GsaI CCCAGC 2 cut(s) 983, 3135
GsuI CTGGAG 2 cut(s) 880, 2741
HaeII RGCGCY 1 cut(s) 3760
HaeIII GGCC 8 cut(s) 769, 1009, 2337, 2378, 2998, 3210, 3741, 3943
HapII CCGG 1 cut(s) 418
HgaI GACGC 1 cut(s) 2855
HhaI GCGC 1 cut(s) 3759
Hin6I GCGC 1 cut(s) 3757
HinP1I GCGC 1 cut(s) 3757
HincII GTYRAC 6 cut(s) 57, 794, 1803, 1959, 2664, 2829
HindII GTYRAC 6 cut(s) 57, 794, 1803, 1959, 2664, 2829
HindIII AAGCTT 5 cut(s) 88, 290, 2204, 2531, 2792
HpaI GTTAAC 1 cut(s) 57
HpaII CCGG 1 cut(s) 418
Hpy166II GTNNAC 8 cut(s) 57, 623, 794, 1803, 1959, 2664, 2692, 2829
Hpy8I GTNNAC 8 cut(s) 57, 623, 794, 1803, 1959, 2664, 2692, 2829
Hpy99I CGWCG 1 cut(s) 2871
HpyCH4IV ACGT 7 cut(s) 866, 946, 1096, 1829, 1947, 2450, 3969
HpyF10VI GCNNNNNNNGC 9 cut(s) 146, 289, 983, 1451, 1975, 2099, 2157, 3207, 3588
HpySE526I ACGT 7 cut(s) 866, 946, 1096, 1829, 1947, 2450, 3969
HspAI GCGC 1 cut(s) 3757
KpnI GGTACC 1 cut(s) 1762
Ksp22I TGATCA 1 cut(s) 3466
KspAI GTTAAC 1 cut(s) 57
LguI GCTCTTC 1 cut(s) 3375
LweI GCATC 9 cut(s) 151, 2015, 2044, 2080, 2951, 3118, 3188, 3836, 3891
MabI ACCWGGT 1 cut(s) 3922
MaeI CTAG 5 cut(s) 896, 2991, 3119, 3140, 3824
MaeII ACGT 7 cut(s) 866, 946, 1096, 1829, 1947, 2450, 3969
MbiI CCGCTC 3 cut(s) 2151, 3368, 4011
MfeI CAATTG 6 cut(s) 1673, 2138, 2634, 3391, 3470, 3577
MflI RGATCY 6 cut(s) 15, 69, 196, 693, 3667, 3673
MhlI GDGCHC 5 cut(s) 866, 979, 1695, 2694, 2938
MlsI TGGCCA 3 cut(s) 2337, 2378, 2998
MluNI TGGCCA 3 cut(s) 2337, 2378, 2998
MlyI GAGTC 9 cut(s) 618, 660, 1808, 1954, 2751, 2897, 3035, 3272, 3639
MmeI TCCRAC 6 cut(s) 483, 2038, 2151, 2418, 3141, 3459
Mox20I TGGCCA 3 cut(s) 2337, 2378, 2998
Mph1103I ATGCAT 1 cut(s) 2525
MroXI GAANNNNTTC 1 cut(s) 2505
MscI TGGCCA 3 cut(s) 2337, 2378, 2998
MslI CAYNNNNRTG 3 cut(s) 2040, 2697, 2836
Msp20I TGGCCA 3 cut(s) 2337, 2378, 2998
MspA1I CMGCKG 4 cut(s) 149, 178, 986, 3901
MspCI CTTAAG 1 cut(s) 1028
MspI CCGG 1 cut(s) 418
MspR9I CCNGG 4 cut(s) 74, 1317, 1728, 3924
MunI CAATTG 6 cut(s) 1673, 2138, 2634, 3391, 3470, 3577
Mva1269I GAATGC 1 cut(s) 29
MvaI CCWGG 4 cut(s) 74, 1317, 1728, 3924
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 9 cut(s) 146, 289, 983, 1451, 1975, 2099, 2157, 3207, 3588
NdeI CATATG 1 cut(s) 496
NheI GCTAGC 2 cut(s) 895, 3823
NlaIV GGNNCC 3 cut(s) 976, 1760, 2047
NmeAIII GCCGAG 3 cut(s) 606, 745, 3992
NmuCI GTSAC 7 cut(s) 733, 937, 1346, 3245, 3251, 3631, 3989
NsiI ATGCAT 1 cut(s) 2525
NspI RCATGY 4 cut(s) 995, 1809, 1921, 2835
NspV TTCGAA 1 cut(s) 3568
OliI CACNNNNGTG 1 cut(s) 2697
PagI TCATGA 1 cut(s) 1195
PceI AGGCCT 1 cut(s) 3741
PciI ACATGT 2 cut(s) 991, 2831
PciSI GCTCTTC 1 cut(s) 3375
PctI GAATGC 1 cut(s) 29
PdmI GAANNNNTTC 1 cut(s) 2505
PfeI GAWTC 3 cut(s) 1406, 2585, 3794
PflFI GACNNNGTC 1 cut(s) 3629
PleI GAGTC 9 cut(s) 618, 659, 1807, 1954, 2751, 2897, 3034, 3271, 3638
PmaCI CACGTG 2 cut(s) 867, 947
PmlI CACGTG 2 cut(s) 867, 947
PpsI GAGTC 9 cut(s) 618, 659, 1807, 1954, 2751, 2897, 3034, 3271, 3638
Ppu21I YACGTR 3 cut(s) 867, 947, 1948
PpuMI RGGWCCY 1 cut(s) 2046
PscI ACATGT 2 cut(s) 991, 2831
PshAI GACNNNNGTC 1 cut(s) 3294
PshBI ATTAAT 1 cut(s) 1277
PsiI TTATAA 2 cut(s) 1710, 2646
Psp5II RGGWCCY 1 cut(s) 2046
Psp6I CCWGG 4 cut(s) 72, 1315, 1726, 3922
PspCI CACGTG 2 cut(s) 867, 947
PspFI CCCAGC 2 cut(s) 979, 3131
PspGI CCWGG 4 cut(s) 72, 1315, 1726, 3922
PspN4I GGNNCC 3 cut(s) 976, 1760, 2047
PspPI GGNCC 3 cut(s) 509, 2046, 3942
PspPPI RGGWCCY 1 cut(s) 2046
PstI CTGCAG 3 cut(s) 117, 1462, 2865
PstNI CAGNNNCTG 3 cut(s) 43, 986, 1733
PsuI RGATCY 6 cut(s) 15, 69, 196, 693, 3667, 3673
PsyI GACNNNGTC 1 cut(s) 3629
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 5 cut(s) 318, 1179, 1682, 1760, 3066
RsaNI GTAC 5 cut(s) 317, 1178, 1681, 1759, 3065
RseI CAYNNNNRTG 3 cut(s) 2040, 2697, 2836
SalI GTCGAC 1 cut(s) 792
SapI GCTCTTC 1 cut(s) 3375
Sau96I GGNCC 3 cut(s) 509, 2046, 3942
ScaI AGTACT 2 cut(s) 1179, 1682
SchI GAGTC 9 cut(s) 618, 660, 1808, 1954, 2751, 2897, 3035, 3272, 3639
ScrFI CCNGG 4 cut(s) 74, 1317, 1728, 3924
SduI GDGCHC 5 cut(s) 866, 979, 1695, 2694, 2938
SexAI ACCWGGT 1 cut(s) 3922
SfaNI GCATC 9 cut(s) 151, 2015, 2044, 2080, 2951, 3118, 3188, 3836, 3891
SfcI CTRYAG 5 cut(s) 113, 669, 1458, 1734, 2861
SfuI TTCGAA 1 cut(s) 3568
SinI GGWCC 2 cut(s) 509, 2046
SmiMI CAYNNNNRTG 3 cut(s) 2040, 2697, 2836
SmlI CTYRAG 6 cut(s) 287, 689, 1028, 1307, 2050, 2939
SmoI CTYRAG 6 cut(s) 287, 689, 1028, 1307, 2050, 2939
SpeI ACTAGT 1 cut(s) 3118
SseBI AGGCCT 1 cut(s) 3741
SspI AATATT 3 cut(s) 1355, 1836, 2881
SspMI CTAG 5 cut(s) 896, 2991, 3119, 3140, 3824
StuI AGGCCT 1 cut(s) 3741
StyD4I CCNGG 4 cut(s) 72, 1315, 1726, 3922
TaiI ACGT 7 cut(s) 869, 949, 1099, 1832, 1950, 2453, 3972
TaqII GACCGA 3 cut(s) 1365, 1733, 3270
TatI WGTACW 2 cut(s) 1177, 1680
TauI GCSGC 3 cut(s) 29, 986, 2154
TfiI GAWTC 3 cut(s) 1406, 2585, 3794
TscAI CASTG 7 cut(s) 12, 198, 678, 1627, 2439, 3899, 3944
TseFI GTSAC 7 cut(s) 733, 937, 1346, 3245, 3251, 3631, 3989
Tsp45I GTSAC 7 cut(s) 733, 937, 1346, 3245, 3251, 3631, 3989
TspGWI ACGGA 2 cut(s) 2527, 2821
TspRI CASTG 7 cut(s) 12, 198, 678, 1627, 2439, 3899, 3944
Tth111I GACNNNGTC 1 cut(s) 3629
Vha464I CTTAAG 1 cut(s) 1028
VneI GTGCAC 1 cut(s) 2690
VpaK11BI GGWCC 2 cut(s) 509, 2046
VspI ATTAAT 1 cut(s) 1277
XapI RAATTY 6 cut(s) 1259, 2243, 2505, 2871, 3106, 3230
XceI RCATGY 4 cut(s) 995, 1809, 1921, 2835
XcmI CCANNNNNNNNNTGG 2 cut(s) 613, 2991
XmiI GTMKAC 1 cut(s) 793
XmnI GAANNNNTTC 1 cut(s) 2505
XspI CTAG 5 cut(s) 896, 2991, 3119, 3140, 3824
ZrmI AGTACT 2 cut(s) 1179, 1682
Zsp2I ATGCAT 1 cut(s) 2525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.