MD00G1070800.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000070
Physical Location & Seq
Forward (+)
13739171 .. 13741132
1962 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1070800.v1.1.491

Sequence Viewer

Length: 1962 bp
ATGTCACTGGGAGAGGTTTTTATTTCTGGATTACTTCAAGTGTTACTTGAAAGGTTGACATCACGTGAAACGCTGAGCTACTTTAGACGCCTACTTGGTGTTGGCAAAGAGCTGAAGAAATGGAAGTCAATGTTGTCTACTATTCGAGCAGTGCTGAATGACGCGGAGGAGAAACAATTGACTAACCAAGAAGTGAAATTGTGGCTGGATGATCTCAGAGACTTGGCCTATGATGTGGAAGACATATTGGACAAGTTTTCAACCGAGATGTTGCGACGCCAAATAAAGGAGAAACATGGGGCTACCACAAGCAAGGTATGGGGTTTGCTTTCTGATGTTAAATTTAACTGTAACCTGAGGTCAGAAATAAAGAAGATTACTGATCGCCTACATGAGATATTGGAAGTGAAAAAACAACTTGGTTTAAATGAGGGGACGCCATCTAAGGCATGGCACGTGCCACCAAGTTCCCGCCTACCGGAAGGACCTGTGATTGGAAGGGATACAGACAAGACGAAGATTATTGACTTGTTGTTAAAAGAAGCACCCAGTGCCATCAACTTTCATGTAGTTGCCATTGTCGGTATGCCTGGAGTGGGAAAGACTACACTTGCAAGACATGTTTATGATGATGACGCAACGAAAGAGTTTAACATAAAAGTATGGGTATCTGTGTCGGATGACTTCAATCTTGAAAGAGTGACAAAGGCAATTCTTGAATCCGCCACATCTGGTCTTGCAAAGGAGTACAAGGAATTCAACCAGGTTCAAGAAAGTTTGAGTAAGGAGCTAGCAGGAAAAAAGTTTCTAATTGTTTTAGACGATGTTTGGAATACATGTGTCTATGATTTGTGGATAAAATTGCAGTCCCCCTTCCGTGTTGGAGCATTAGGAAGTAAGATAATTGTGACAACACGTGATGCAAATGTTGCAAACATGATGGGATCCACTGTTTATCAATTGGATTGCATGACAGGTGATCAATGTTGGGAAGTCTTTGAGCAACATTCCCGATTGGACATTACCAAAAGGCCGCAAAAGTTTGAGTTGATAAAGGAGAAAATTGTTGCAAAATGTCGCGGATTGCCATTGGCTGCAAGGACTCTTGGAGGTCTTCTACGCTGTAAACAAGTAGAAGAATGGGAGGAAATCTTAAACAACAAGATGTGGAGTCTATCAGACAATAGTGGCATTCTCCCAGTATTAAAATTGAGCTATCACTATCTCCCTTCGAGTTTGAAACGGTGTTTCGCCTATTGTTCCCTACTTCCAAATGACCATGAGTTCAGGGAGAAGCAGTTGATCCTTCTGTGGATGGCAGAAGGTTTGATTCAACAAAGAGTAGAGGACAAGAAACAAGTGGAAGAAGTAGGCAGTGACTATTTTCAAGAGCTATTGTCCAGGTCATTATTTCAAAAGGCAAGCAAAAGCAATGATAAGTATGTAATGCATGACCTTGTTGGTGAGTTGGCGCGGTGGGCTGCAGCTGAAATATGCTTTTCATTGGAGGATAAGGGAAATGATGACTTGCATCCAATGGCTCGTCATATTTCTTATGTTATTGGTGAGTATGATGGGGTTAAAAACTTTGAGGCCATTTCAAGAGTTAAACATTTGAGGACATTCCTTCCATTTCAACAATCTTTATCTTGGAACCATCTAACTCGTAGGGTTACATTTGATCTATTGCCAGAACTGCAAATCTTACGGGTGCTTTCTTTGAATGGCTATAAAATAACTGAGTTGCCCAATTCAATTGGTAAATTGTGGAATCTACGGTATCTGGACCTTTCTTATACATGGATAAAGAGTTTGCCTCCATCAACAACCACTCTTTGCAATCTGCAAACATTGTTATTAGAAGGTTGTTATGAATTGGAGGTATTGCCCGCAAACATGAGTACAACAACAACAACAACAAAGCCTTTTCCCACTAAGTGGGGTCGGCTATATGAATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

654

Amino Acids

74.83

Weight (kDa)

7.88

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 99 1.5e-25 Rx N-terminal domain
NB-ARC PF00931 168 - 335 1.9e-35 NB-ARC domain
WHD_DRP PF23559 423 - 491 3.6e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 554 - 626 1.4e-11 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1938
AccI GTMKAC 1 cut(s) 137
AccII CGCG 3 cut(s) 164, 1080, 1474
AciI CCGC 7 cut(s) 164, 472, 723, 1034, 1080, 1474, 1890
AclWI GGATC 3 cut(s) 939, 952, 1297
AcsI RAATTY 2 cut(s) 341, 755
AcuI CTGAAG 1 cut(s) 134
AcvI CACGTG 3 cut(s) 65, 457, 917
AcyI GRCGYC 3 cut(s) 88, 277, 437
AdeI CACNNNGTG 2 cut(s) 551, 1938
AfaI GTAC 2 cut(s) 749, 1903
AfiI CCNNNNNNNGG 5 cut(s) 286, 478, 494, 596, 1938
AflIII ACRYGT 3 cut(s) 619, 836, 914
AjnI CCWGG 3 cut(s) 589, 762, 1400
AluBI AGCT 6 cut(s) 78, 112, 790, 1215, 1393, 1487
AluI AGCT 6 cut(s) 78, 112, 790, 1215, 1393, 1487
Alw26I GTCTC 1 cut(s) 213
AlwI GGATC 3 cut(s) 939, 952, 1297
AoxI GGCC 3 cut(s) 225, 1031, 1593
ApeKI GCWGC 3 cut(s) 1094, 1481, 1484
ApoI RAATTY 2 cut(s) 341, 755
AspLEI GCGC 1 cut(s) 1474
AspS9I GGNCC 2 cut(s) 485, 1786
AsuHPI GGTGA 3 cut(s) 989, 1475, 1577
AsuNHI GCTAGC 1 cut(s) 790
AvaII GGWCC 2 cut(s) 485, 1786
AxyI CCTNAGG 1 cut(s) 356
BamHI GGATCC 1 cut(s) 944
BbrPI CACGTG 3 cut(s) 65, 457, 917
BbsI GAAGAC 2 cut(s) 246, 1106
BbvI GCAGC 3 cut(s) 1081, 1468, 1496
BccI CCATC 7 cut(s) 448, 563, 934, 1309, 1568, 1665, 1828
BciT130I CCWGG 3 cut(s) 591, 764, 1402
BciVI GTATCC 1 cut(s) 496
BclI TGATCA 1 cut(s) 979
BcoDI GTCTC 1 cut(s) 213
BfaI CTAG 2 cut(s) 791, 1960
BfmI CTRYAG 1 cut(s) 1482
BfuI GTATCC 1 cut(s) 496
BisI GCNGC 4 cut(s) 1034, 1095, 1482, 1485
BlpI GCTNAGC 1 cut(s) 74
BlsI GCNGC 4 cut(s) 1035, 1096, 1483, 1486
Bme1390I CCNGG 3 cut(s) 591, 764, 1402
Bme18I GGWCC 2 cut(s) 485, 1786
BmgT120I GGNCC 2 cut(s) 485, 1786
BmiI GGNNCC 2 cut(s) 946, 1655
BmrFI CCNGG 3 cut(s) 591, 764, 1402
BmrI ACTGGG 3 cut(s) 17, 543, 1193
BmsI GCATC 2 cut(s) 910, 1540
BmtI GCTAGC 1 cut(s) 794
BmuI ACTGGG 3 cut(s) 17, 543, 1193
BpiI GAAGAC 2 cut(s) 246, 1106
BplI GAGNNNNNCTC 2 cut(s) 1801, 1833
BpmI CTGGAG 1 cut(s) 612
Bpu1102I GCTNAGC 1 cut(s) 74
BsaAI YACGTR 3 cut(s) 65, 457, 917
BsaHI GRCGYC 3 cut(s) 88, 277, 437
BsaWI WCCGGW 1 cut(s) 478
BsaXI ACNNNNNCTCC 2 cut(s) 1283, 1313
Bsc4I CCNNNNNNNGG 5 cut(s) 286, 478, 494, 596, 1938
Bse1I ACTGG 3 cut(s) 12, 549, 1199
Bse21I CCTNAGG 1 cut(s) 356
Bse3DI GCAATG 1 cut(s) 1438
BseBI CCWGG 3 cut(s) 591, 764, 1402
BseGI GGATG 4 cut(s) 214, 685, 1320, 1531
BseLI CCNNNNNNNGG 5 cut(s) 286, 478, 494, 596, 1938
BseMI GCAATG 1 cut(s) 1438
BseMII CTCAG 4 cut(s) 65, 229, 347, 1731
BseNI ACTGG 3 cut(s) 12, 549, 1199
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 3 cut(s) 1081, 1468, 1496
Bsh1236I CGCG 3 cut(s) 164, 1080, 1474
BshFI GGCC 3 cut(s) 227, 1033, 1595
BsiSI CCGG 1 cut(s) 479
BslFI GGGAC 2 cut(s) 448, 853
BslI CCNNNNNNNGG 5 cut(s) 286, 478, 494, 596, 1938
BsmAI GTCTC 1 cut(s) 213
BsmFI GGGAC 2 cut(s) 448, 853
BsmI GAATGC 1 cut(s) 1191
BsnI GGCC 3 cut(s) 227, 1033, 1595
Bsp143I GATC 6 cut(s) 211, 382, 944, 979, 1302, 1681
Bsp1720I GCTNAGC 1 cut(s) 74
BspACI CCGC 7 cut(s) 164, 472, 723, 1034, 1080, 1474, 1890
BspANI GGCC 3 cut(s) 227, 1033, 1595
BspCNI CTCAG 4 cut(s) 66, 228, 348, 1732
BspFNI CGCG 3 cut(s) 164, 1080, 1474
BspLI GGNNCC 2 cut(s) 946, 1655
BspMAI CTGCAG 1 cut(s) 1486
BspOI GCTAGC 1 cut(s) 794
BspPI GGATC 3 cut(s) 939, 952, 1297
BsrDI GCAATG 1 cut(s) 1438
BsrI ACTGG 3 cut(s) 12, 549, 1199
BssMI GATC 6 cut(s) 211, 382, 944, 979, 1302, 1681
BssNI GRCGYC 3 cut(s) 88, 277, 437
Bst2UI CCWGG 3 cut(s) 591, 764, 1402
Bst4CI ACNGT 4 cut(s) 350, 952, 1245, 1779
BstACI GRCGYC 3 cut(s) 88, 277, 437
BstAPI GCANNNNNTGC 2 cut(s) 551, 929
BstBAI YACGTR 3 cut(s) 65, 457, 917
BstC8I GCNNGC 3 cut(s) 792, 1423, 1890
BstDEI CTNAG 6 cut(s) 74, 215, 356, 444, 1740, 1935
BstF5I GGATG 4 cut(s) 214, 685, 1320, 1531
BstFNI CGCG 3 cut(s) 164, 1080, 1474
BstHHI GCGC 1 cut(s) 1474
BstKTI GATC 6 cut(s) 214, 385, 947, 982, 1305, 1684
BstMAI GTCTC 1 cut(s) 213
BstMBI GATC 6 cut(s) 211, 382, 944, 979, 1302, 1681
BstMWI GCNNNNNNNGC 4 cut(s) 551, 929, 1478, 1696
BstNI CCWGG 3 cut(s) 591, 764, 1402
BstNSI RCATGY 2 cut(s) 623, 840
BstSCI CCNGG 3 cut(s) 589, 762, 1400
BstSFI CTRYAG 1 cut(s) 1482
BstUI CGCG 3 cut(s) 164, 1080, 1474
BstV1I GCAGC 3 cut(s) 1081, 1468, 1496
BstV2I GAAGAC 2 cut(s) 246, 1106
BstX2I RGATCY 1 cut(s) 944
BstYI RGATCY 1 cut(s) 944
Bsu36I CCTNAGG 1 cut(s) 356
BsuI GTATCC 1 cut(s) 496
BsuRI GGCC 3 cut(s) 227, 1033, 1595
BtsCI GGATG 4 cut(s) 214, 685, 1320, 1531
BtsI GCAGTG 2 cut(s) 156, 1381
BtsIMutI CAGTG 5 cut(s) 5, 156, 556, 948, 1381
Cac8I GCNNGC 3 cut(s) 792, 1423, 1890
CfoI GCGC 1 cut(s) 1474
Cfr13I GGNCC 2 cut(s) 485, 1786
CseI GACGC 5 cut(s) 96, 170, 285, 445, 644
CsiI ACCWGGT 1 cut(s) 762
Csp6I GTAC 2 cut(s) 748, 1902
CviQI GTAC 2 cut(s) 748, 1902
DdeI CTNAG 6 cut(s) 74, 215, 356, 444, 1740, 1935
DpnI GATC 6 cut(s) 213, 384, 946, 981, 1304, 1683
DpnII GATC 6 cut(s) 211, 382, 944, 979, 1302, 1681
DraI TTTAAA 1 cut(s) 426
DraIII CACNNNGTG 2 cut(s) 551, 1938
EciI GGCGGA 1 cut(s) 712
Eco47I GGWCC 2 cut(s) 485, 1786
Eco57I CTGAAG 1 cut(s) 134
Eco72I CACGTG 3 cut(s) 65, 457, 917
Eco81I CCTNAGG 1 cut(s) 356
EcoO109I RGGNCCY 1 cut(s) 485
EcoRI GAATTC 1 cut(s) 755
EcoRII CCWGG 3 cut(s) 589, 762, 1400
EcoT22I ATGCAT 1 cut(s) 1452
FalI AAGNNNNNCTT 2 cut(s) 30, 62
FaqI GGGAC 2 cut(s) 448, 853
FauI CCCGC 2 cut(s) 479, 1897
FbaI TGATCA 1 cut(s) 979
FblI GTMKAC 1 cut(s) 137
Fnu4HI GCNGC 4 cut(s) 1034, 1095, 1482, 1485
FokI GGATG 4 cut(s) 221, 692, 1327, 1518
Fsp4HI GCNGC 4 cut(s) 1034, 1095, 1482, 1485
FspBI CTAG 2 cut(s) 791, 1960
GlaI GCGC 1 cut(s) 1473
GluI GCNGC 4 cut(s) 1034, 1095, 1482, 1485
GsuI CTGGAG 1 cut(s) 612
HaeIII GGCC 3 cut(s) 227, 1033, 1595
HapII CCGG 1 cut(s) 479
HgaI GACGC 5 cut(s) 96, 170, 285, 445, 644
HhaI GCGC 1 cut(s) 1474
Hin1I GRCGYC 3 cut(s) 88, 277, 437
Hin6I GCGC 1 cut(s) 1472
HinP1I GCGC 1 cut(s) 1472
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HinfI GANTC 6 cut(s) 719, 1102, 1171, 1330, 1771, 1955
HpaII CCGG 1 cut(s) 479
HphI GGTGA 3 cut(s) 989, 1475, 1577
Hpy166II GTNNAC 3 cut(s) 57, 138, 1127
Hpy188I TCNGA 5 cut(s) 218, 334, 364, 679, 1180
Hpy188III TCNNGA 8 cut(s) 27, 692, 716, 770, 1011, 1388, 1602, 1784
Hpy8I GTNNAC 3 cut(s) 57, 138, 1127
Hpy99I CGWCG 1 cut(s) 279
HpyAV CCTTC 8 cut(s) 476, 492, 883, 1239, 1316, 1316, 1637, 1856
HpyCH4III ACNGT 4 cut(s) 350, 952, 1245, 1779
HpyCH4IV ACGT 3 cut(s) 64, 456, 916
HpyF10VI GCNNNNNNNGC 4 cut(s) 551, 929, 1478, 1696
HpyF3I CTNAG 6 cut(s) 74, 215, 356, 444, 1740, 1935
HpySE526I ACGT 3 cut(s) 64, 456, 916
Hsp92I GRCGYC 3 cut(s) 88, 277, 437
HspAI GCGC 1 cut(s) 1472
Ksp22I TGATCA 1 cut(s) 979
Kzo9I GATC 6 cut(s) 211, 382, 944, 979, 1302, 1681
LmnI GCTCC 2 cut(s) 787, 884
Lsp1109I GCAGC 3 cut(s) 1081, 1468, 1496
LweI GCATC 2 cut(s) 910, 1540
MabI ACCWGGT 1 cut(s) 762
MaeI CTAG 2 cut(s) 791, 1960
MaeII ACGT 3 cut(s) 64, 456, 916
MaeIII GTNAC 7 cut(s) 3, 42, 350, 700, 907, 1376, 1672
MalI GATC 6 cut(s) 213, 384, 946, 981, 1304, 1683
MboI GATC 6 cut(s) 211, 382, 944, 979, 1302, 1681
MboII GAAGA 7 cut(s) 127, 251, 385, 529, 1106, 1148, 1376
MfeI CAATTG 3 cut(s) 176, 959, 1755
MflI RGATCY 1 cut(s) 944
MlyI GAGTC 2 cut(s) 1096, 1180
MmeI TCCRAC 2 cut(s) 657, 862
Mph1103I ATGCAT 1 cut(s) 1452
MseI TTAA 9 cut(s) 339, 345, 425, 536, 651, 1154, 1205, 1581, 1608
MslI CAYNNNNRTG 1 cut(s) 624
MspA1I CMGCKG 1 cut(s) 1487
MspI CCGG 1 cut(s) 479
MspR9I CCNGG 3 cut(s) 591, 764, 1402
MunI CAATTG 3 cut(s) 176, 959, 1755
Mva1269I GAATGC 1 cut(s) 1191
MvaI CCWGG 3 cut(s) 591, 764, 1402
MvnI CGCG 3 cut(s) 164, 1080, 1474
MwoI GCNNNNNNNGC 4 cut(s) 551, 929, 1478, 1696
NdeII GATC 6 cut(s) 211, 382, 944, 979, 1302, 1681
NheI GCTAGC 1 cut(s) 790
NlaIV GGNNCC 2 cut(s) 946, 1655
NmuCI GTSAC 4 cut(s) 3, 700, 907, 1376
NsiI ATGCAT 1 cut(s) 1452
NspI RCATGY 2 cut(s) 623, 840
PciI ACATGT 2 cut(s) 619, 836
PctI GAATGC 1 cut(s) 1191
PfeI GAWTC 4 cut(s) 719, 1330, 1771, 1955
PflMI CCANNNNNTGG 1 cut(s) 1938
PkrI GCNGC 4 cut(s) 1035, 1096, 1483, 1486
PleI GAGTC 2 cut(s) 1096, 1179
PmaCI CACGTG 3 cut(s) 65, 457, 917
PmlI CACGTG 3 cut(s) 65, 457, 917
PpsI GAGTC 2 cut(s) 1096, 1179
Ppu21I YACGTR 3 cut(s) 65, 457, 917
PpuMI RGGWCCY 1 cut(s) 485
PscI ACATGT 2 cut(s) 619, 836
Psp5II RGGWCCY 1 cut(s) 485
Psp6I CCWGG 3 cut(s) 589, 762, 1400
PspCI CACGTG 3 cut(s) 65, 457, 917
PspGI CCWGG 3 cut(s) 589, 762, 1400
PspN4I GGNNCC 2 cut(s) 946, 1655
PspPI GGNCC 2 cut(s) 485, 1786
PspPPI RGGWCCY 1 cut(s) 485
PstI CTGCAG 1 cut(s) 1486
PsuI RGATCY 1 cut(s) 944
PvuII CAGCTG 1 cut(s) 1487
RsaI GTAC 2 cut(s) 749, 1903
RsaNI GTAC 2 cut(s) 748, 1902
RseI CAYNNNNRTG 1 cut(s) 624
SaqAI TTAA 9 cut(s) 339, 345, 425, 536, 651, 1154, 1205, 1581, 1608
SatI GCNGC 4 cut(s) 1034, 1095, 1482, 1485
Sau3AI GATC 6 cut(s) 211, 382, 944, 979, 1302, 1681
Sau96I GGNCC 2 cut(s) 485, 1786
SchI GAGTC 2 cut(s) 1096, 1180
ScrFI CCNGG 3 cut(s) 591, 764, 1402
SexAI ACCWGGT 1 cut(s) 762
SfaNI GCATC 2 cut(s) 910, 1540
SfcI CTRYAG 1 cut(s) 1482
SinI GGWCC 2 cut(s) 485, 1786
SmiMI CAYNNNNRTG 1 cut(s) 624
SsiI CCGC 7 cut(s) 164, 472, 723, 1034, 1080, 1474, 1890
SspMI CTAG 2 cut(s) 791, 1960
StyD4I CCNGG 3 cut(s) 589, 762, 1400
TaaI ACNGT 4 cut(s) 350, 952, 1245, 1779
TaiI ACGT 3 cut(s) 67, 459, 919
TaqI TCGA 2 cut(s) 145, 1232
TatI WGTACW 2 cut(s) 747, 1901
TauI GCSGC 1 cut(s) 1036
TfiI GAWTC 4 cut(s) 719, 1330, 1771, 1955
Tru1I TTAA 9 cut(s) 339, 345, 425, 536, 651, 1154, 1205, 1581, 1608
Tru9I TTAA 9 cut(s) 339, 345, 425, 536, 651, 1154, 1205, 1581, 1608
TscAI CASTG 5 cut(s) 12, 156, 556, 955, 1381
TseFI GTSAC 4 cut(s) 3, 700, 907, 1376
TseI GCWGC 3 cut(s) 1094, 1481, 1484
Tsp45I GTSAC 4 cut(s) 3, 700, 907, 1376
TspDTI ATGAA 3 cut(s) 554, 1491, 1887
TspGWI ACGGA 1 cut(s) 866
TspRI CASTG 5 cut(s) 12, 156, 556, 955, 1381
Van91I CCANNNNNTGG 1 cut(s) 1938
VpaK11BI GGWCC 2 cut(s) 485, 1786
XapI RAATTY 2 cut(s) 341, 755
XceI RCATGY 2 cut(s) 623, 840
XcmI CCANNNNNNNNNTGG 1 cut(s) 447
XmiI GTMKAC 1 cut(s) 137
XspI CTAG 2 cut(s) 791, 1960
Zsp2I ATGCAT 1 cut(s) 1452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.