Rw0G012580

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00562
OG0000070
Physical Location & Seq
Reverse (-)
1191 .. 3197
2007 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G012580.1

Sequence Viewer

Length: 1875 bp
ATGGGAGGACTGGGCAAGACAACGCTTGCGGGACAAGTCTTCAATGATGTAGCTGCAATGGAACAATTTGATCTCAGGATCTGGGTTTCAGTCTCCGATGACTTCGATCTTCAAATGGTGACGAGAACTATTTCTGGAAACGTCACATCTACGCCGTGTGATGATTTCAGTCGACTTCAAGATAATCTGAGCAAGGCGATAGATGGTAAAAAGTTTTTGATTGTTTTAGATGATGTCTGGAGCACGTGTGGTTATGATTCATGGACAAAACTGCGAGCTCCCTTCGGTGGGGGAGCTAAGGGAAGTAGGATACTAGTGACAACACGTGATGAAAAGGTGGCAGCATTGATGGGAGCCCCAGCCGCTGAACATGTTAGCAACGACAGGCCACCGAATTTTGACTTGCTTAAGAAGAAAATTATTACGAAATGCATTGGATTACCATTGGCCGCAAAAACTCTTGGTGGTGTTTTACGCTGTAAAGAAACTGACAAATGGGAGGAAATACTGAATGACAAATTGAGTTATAATTATCTCCCTTCAACTTTGAAGAGGTGCTTTGCCTATTGCTCAGTACTTCCCAATAATTATGAATTTGGGAAAAACCAATTAATCCTTCTATGGATGGCAGAGGGTTTCCTTGAGCAACCAGAAGGAGCTAAAGCAATGGAAGATATCGGTGACGAATATTTTGAGGAGCTATTGTCTCGGTCATTATTTCAAAATTCGGGCAAAAACAGTTCACATTATGTAATGCATGACCTTGTTGGTGATTTAGCACGATGGGCTGCTGGAGACACATTTTGCAGATTAGAGGATAAACCGCATGGTAGATGTTCTCCAACGACTCGTCATCTGACTTACATTTCTGGTAAGTTCGATGGGATAAAACGATTTGAGACACTTTCTGATGCAAAATGCTTGCGGACTTTCCTTCCACTTTTAGTGTCCAATGGTTATGAGAATTATCTAACTCGTTATGCTACTTCTGATTTATTGTCACAATTGAAGTACTTGCGGGTGCTCTCCTTCAATGGGTATAAACTGATCGAGGTGCTAGATTCCGTATGTAATTTGAAACATCTACGGTACCTTGATCTTTCTCACACATTGATAATGAGTTTGCCTGAGTTAATATGCATGCTTTACAACTTACAGACGTTAATATTAGAGAATTGTTCAAAATTGAAGACACTACCTTCAAACATGAGCAATTTATCTAATTTACGTCATCTCAACAATTCAAATACGCCTTTATTGGAAGAAATGCCGCCCCAATTAAAGTTAGGGTCATTGCATCTTCTTGGGACCTTGAGGCTTGAAAGATTGGAGAATGTGATTGATGTTGAGGATGCAAAGAGGGCCCAAATAATGAACAAGGAAGCGCTGGAAACCTTGCGATTGGAATGGAGTGGCACAAGTGAGAAGGAGTCGGAGGTGCTCTGCAGTTTAAAACCTCAGAAAAAGCTTCTAGAGCTCACCATCAAGGGTTACAATGGTTTAGAATTCTCAAAATGGATTGGACATCCTTCATTCTCTGATATGACGTATGTGAAGTTACAGAAATGTAAAAATTGTCGATTCTTACCTCCTCTTGGCCAATTACCTTTACTAAAAGTCCTTTGGATAAAAGGACTGGCCAATGTTGAAAGTGTGGGTGCTGAGTTTTATGGAGATTGTAGCTTGCCTTTTCCAGTGTTGGAAGAGCTGAGGTTCGAGGATATGCAAAATTGGAAGGAGTGGCTTCCTTGCAAAAGAAATGAAGAAATTCGAATTTTCCCATGCATGGGAAAACTTTTCATCCGTAGTTGCCCAAGTCTGAAAGGTTGGTTGCCCAAGAACATGGATTTCATTATCAAAGCTGCGTATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

624

Amino Acids

70.83

Weight (kDa)

7.73

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 1 - 124 1.6e-26 NB-ARC domain
WHD_DRP PF23559 193 - 260 4.9e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 332 - 558 9.6e-18 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 429 - 545 3.3e-30 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000070)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g00360 FvH4_7g00850 FvH4_7g02400 FvH4_7g02430 FvH4_7g02450 FvH4_7g25671 FvH4_7g25680 FvH4_7g25683 FvH4_7g26980 FvH4_7g27330 FvH4_7g27355 FvH4_7g27610 FvH4_7g27642 FvH4_7g27890 FvH4_7g27891 FvH4_7g27891 FvH4_7g27891 FvH4_7g28110 FvH4_7g28390
malus_domestica MD00G1070800.v1.1 MD00G1168000.v1.1 MD00G1168300.v1.1 MD00G1168700.v1.1 MD02G1293100.v1.1 MD02G1293200.v1.1 MD02G1314300.v1.1 MD03G1009000.v1.1 MD03G1009400.v1.1 MD03G1042300.v1.1 MD03G1042400.v1.1 MD07G1033600.v1.1 MD07G1033700.v1.1 MD07G1033900.v1.1 MD11G1011400.v1.1 MD11G1011700.v1.1 MD11G1029800.v1.1 MD11G1029900.v1.1 MD11G1030300.v1.1 MD11G1030400.v1.1 MD11G1030500.v1.1 MD11G1031300.v1.1 MD11G1031600.v1.1 MD11G1032000.v1.1 MD11G1033200.v1.1 MD11G1033600.v1.1 MD11G1033800.v1.1 MD11G1033900.v1.1 MD11G1034100.v1.1 MD11G1034400.v1.1 MD11G1040600.v1.1 MD11G1041100.v1.1 MD11G1043100.v1.1 MD11G1043500.v1.1 MD11G1044500.v1.1 MD11G1045100.v1.1 MD11G1045600.v1.1
prunus_persica Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022400_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022500_v2.0.a1 Prupe.2G022900_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023500_v2.0.a1 Prupe.2G023600_v2.0.a1 Prupe.2G028000_v2.0.a1 Prupe.2G030200_v2.0.a1 Prupe.2G067600_v2.0.a1 Prupe.8G117900_v2.0.a1
pyrus_communis pycom02g26350 pycom03g03270 pycom07g02370 pycom11g00750 pycom11g00790 pycom11g02340 pycom11g02370 pycom11g02380 pycom11g02390 pycom11g02410 pycom11g02460 pycom11g02470 pycom11g02500 pycom11g02510 pycom11g02520 pycom11g02530 pycom11g02580 pycom11g02590 pycom11g02680 pycom11g02710 pycom11g02720 pycom11g02730 pycom11g02750 pycom11g02760 pycom11g02770 pycom11g03690 pycom13g25120 pycom13g25130 pycom13g25140 pycom13g25170 pycom13g25180 pycom13g25190 pycom13g25210
rosa_chinensis RchiOBHm_Chr1g0315001 RchiOBHm_Chr1g0315411 RchiOBHm_Chr1g0317951 RchiOBHm_Chr1g0319741 RchiOBHm_Chr1g0319841 RchiOBHm_Chr1g0319911 RchiOBHm_Chr1g0319991 RchiOBHm_Chr1g0320001 RchiOBHm_Chr1g0320021 RchiOBHm_Chr1g0320081 RchiOBHm_Chr1g0320111 RchiOBHm_Chr1g0320131 RchiOBHm_Chr1g0320161 RchiOBHm_Chr1g0320171 RchiOBHm_Chr1g0320241 RchiOBHm_Chr1g0320281 RchiOBHm_Chr1g0320411 RchiOBHm_Chr1g0320461 RchiOBHm_Chr1g0320551 RchiOBHm_Chr1g0320581 RchiOBHm_Chr1g0320661 RchiOBHm_Chr1g0320891 RchiOBHm_Chr1g0320971 RchiOBHm_Chr1g0321021 RchiOBHm_Chr1g0321031 RchiOBHm_Chr1g0321091 RchiOBHm_Chr1g0321351 RchiOBHm_Chr1g0321481 RchiOBHm_Chr1g0321491 RchiOBHm_Chr1g0327521 RchiOBHm_Chr1g0328061 RchiOBHm_Chr1g0334991 RchiOBHm_Chr1g0373781 RchiOBHm_Chr1g0373891 RchiOBHm_Chr1g0374841 RchiOBHm_Chr2g0131111 RchiOBHm_Chr2g0131121 RchiOBHm_Chr4g0408901 RchiOBHm_Chr5g0041691 RchiOBHm_Chr7g0237021
rosa_laevigata RLG00000019168 RLG00000030456 RLG00000030460 RLG00000030571
rosa_multiflora Rmu_co7963468.1_g000001 Rmu_co7991248.1_g000001 Rmu_co8195256.1_g000001 Rmu_co8312199.1_g000001 Rmu_co8349215.1_g000001 Rmu_co8351041.1_g000001 Rmu_co8422719.1_g000001 Rmu_co8450711.1_g000001 Rmu_co8465555.1_g000001 Rmu_co8489965.1_g000001 Rmu_sc0000087.1_g000014 Rmu_sc0000272.1_g000015 Rmu_sc0000724.1_g000006 Rmu_sc0000724.1_g000021 Rmu_sc0000724.1_g000022 Rmu_sc0000861.1_g000024 Rmu_sc0001049.1_g000006 Rmu_sc0001049.1_g000007 Rmu_sc0001512.1_g000001 Rmu_sc0002022.1_g000004 Rmu_sc0002063.1_g000011 Rmu_sc0002131.1_g000024 Rmu_sc0002337.1_g000012 Rmu_sc0002341.1_g000005 Rmu_sc0002539.1_g000117 Rmu_sc0002745.1_g000005 Rmu_sc0002745.1_g000006 Rmu_sc0003145.1_g000002 Rmu_sc0003199.1_g000007 Rmu_sc0003270.1_g000039 Rmu_sc0003270.1_g000040 Rmu_sc0003423.1_g000001 Rmu_sc0003423.1_g000002 Rmu_sc0003629.1_g000067 Rmu_sc0004039.1_g000002 Rmu_sc0004555.1_g000001 Rmu_sc0004555.1_g000005 Rmu_sc0004555.1_g000006 Rmu_sc0004947.1_g000025 Rmu_sc0004947.1_g000026 Rmu_sc0005255.1_g000025 Rmu_sc0007977.1_g000016 Rmu_sc0008752.1_g000005 Rmu_sc0009236.1_g000004 Rmu_sc0009236.1_g000008 Rmu_sc0009236.1_g000009 Rmu_sc0009236.1_g000010 Rmu_sc0011424.1_g000036 Rmu_sc0013413.1_g000001 Rmu_sc0013735.1_g000002 Rmu_sc0015304.1_g000002 Rmu_sc0015304.1_g000003 Rmu_sc0015585.1_g000001 Rmu_sc0018410.1_g000001 Rmu_sc0022723.1_g000001 Rmu_sc0022723.1_g000002 Rmu_sc0022724.1_g000001 Rmu_sc0023430.1_g000001 Rmu_sc0026053.1_g000001 Rmu_sc0026429.1_g000002 Rmu_sc0026647.1_g000001 Rmu_sc0031359.1_g000001 Rmu_ssc0000042.1_g000040 Rmu_ssc0000081.1_g000001 Rmu_ssc0000300.1_g000011
rosa_roxburghii Rroxscaffold_160G00433620 Rroxscaffold_160G00433990 Rroxscaffold_1G00038820 Rroxscaffold_2G00113190 Rroxscaffold_4G00283240 Rroxscaffold_4G00283930 Rroxscaffold_4G00284010 Rroxscaffold_4G00322600 Rroxscaffold_4G00327710 Rroxscaffold_4G00328100 Rroxscaffold_4G00328130 Rroxscaffold_4G00331590 Rroxscaffold_5G00353280
rosa_rugosa Rorug01G0004200 Rorug01G0018800 Rorug01G0018900 Rorug01G0029900 Rorug01G0030000 Rorug01G0030000 Rorug01G0030000 Rorug01G0030200 Rorug01G0030300 Rorug01G0030800 Rorug01G0032400 Rorug01G0032900 Rorug01G0055800 Rorug01G0067600 Rorug01G0067700 Rorug01G0067800 Rorug01G0067900 Rorug01G0068000 Rorug01G0379500 Rorug01G0379600 Rorug01G0379600 Rorug01G0385200 Rorug01G0385300 Rorug02G0295100 Rorug04G0088500 Rorug04G0088600 Rorug05G0194900 Rorug07G0301400
rosa_samantha Rh1AG014800 Rh1AG015200 Rh1AG030500 Rh1AG041800 Rh1AG042300 Rh1AG042700 Rh1AG042800 Rh1AG042900 Rh1AG045000 Rh1AG085100 Rh1AG085200 Rh1AG388600 Rh1AG396700 Rh1AG396900 Rh1BG007300 Rh1BG039200 Rh1BG039600 Rh1BG042800 Rh1BG067300 Rh1BG109700 Rh1BG109900 Rh1BG354000 Rh1CG043800 Rh1CG044400 Rh1DG008600 Rh1DG022900 Rh1DG023400 Rh1DG023600 Rh1DG023900 Rh1DG024100 Rh1DG024400 Rh1DG024500 Rh1DG025500 Rh1DG025700 Rh1DG026500 Rh1DG026800 Rh1DG038900 Rh1DG048800 Rh1DG049300 Rh1DG051700 Rh1DG052000 Rh1DG146200 Rh1DG383500 Rh2BG354900 Rh2CG333600 Rh4BG149600 Rh4BG149700 Rh4CG160600
rosa_wichuraiana Rw0G001100 Rw0G001130 Rw0G001180 Rw0G012580 Rw1G003480 Rw1G003540 Rw1G003600 Rw1G003640 Rw1G003670 Rw1G003750 Rw1G003940 Rw1G006650 Rw1G034550 Rw1G034560 Rw1G034650 Rw1G034660 Rw1G035170 Rw1G035190 Rw1G037060 Rw2G028210 Rw4G012540 Rw5G026370 Rw7G037900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 528
Acc65I GGTACC 1 cut(s) 1089
AccB1I GGYRCC 1 cut(s) 1089
AccI GTMKAC 1 cut(s) 172
AciI CCGC 7 cut(s) 29, 363, 450, 824, 925, 1018, 1271
AclWI GGATC 1 cut(s) 86
AcoI YGGCCR 3 cut(s) 447, 1597, 1638
AcsI RAATTY 6 cut(s) 394, 593, 724, 1505, 1767, 1773
AcvI CACGTG 2 cut(s) 246, 326
AfaI GTAC 3 cut(s) 576, 1013, 1091
AfeI AGCGCT 1 cut(s) 1386
AfiI CCNNNNNNNGG 6 cut(s) 287, 288, 1035, 1595, 1786, 1787
AflII CTTAAG 1 cut(s) 407
AflIII ACRYGT 3 cut(s) 245, 323, 370
AhlI ACTAGT 1 cut(s) 313
AjuI GAANNNNNNNTTGG 2 cut(s) 600, 632
Alw21I GWGCWC 5 cut(s) 245, 280, 1026, 1443, 1479
Alw26I GTCTC 4 cut(s) 97, 711, 789, 893
AlwI GGATC 1 cut(s) 86
AlwNI CAGNNNCTG 2 cut(s) 81, 365
Aor51HI AGCGCT 1 cut(s) 1386
AoxI GGCC 5 cut(s) 386, 447, 1362, 1597, 1638
ApaI GGGCCC 1 cut(s) 1366
ApeKI GCWGC 4 cut(s) 53, 341, 788, 1862
ApoI RAATTY 6 cut(s) 394, 593, 724, 1505, 1767, 1773
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
AseI ATTAAT 1 cut(s) 611
Asp700I GAANNNNTTC 2 cut(s) 130, 1767
Asp718I GGTACC 1 cut(s) 1089
AspLEI GCGC 1 cut(s) 1387
AspS9I GGNCC 3 cut(s) 1308, 1362, 1363
AsuHPI GGTGA 4 cut(s) 130, 692, 782, 1471
AsuII TTCGAA 1 cut(s) 1771
AvaII GGWCC 1 cut(s) 1308
BaeGI GKGCMC 1 cut(s) 1366
BalI TGGCCA 2 cut(s) 1599, 1640
BanI GGYRCC 1 cut(s) 1089
BanII GRGCYC 4 cut(s) 280, 358, 1366, 1479
BbrPI CACGTG 2 cut(s) 246, 326
BbsI GAAGAC 2 cut(s) 31, 1196
Bbv12I GWGCWC 5 cut(s) 245, 280, 1026, 1443, 1479
BbvCI CCTCAGC 1 cut(s) 1709
BbvI GCAGC 4 cut(s) 40, 353, 775, 1849
BccI CCATC 6 cut(s) 197, 343, 619, 777, 875, 1490
BceAI ACGGC 1 cut(s) 139
BciVI GTATCC 1 cut(s) 303
BcoDI GTCTC 4 cut(s) 97, 711, 789, 893
BcuI ACTAGT 1 cut(s) 313
BfaI CTAG 3 cut(s) 314, 1058, 1472
BfmI CTRYAG 1 cut(s) 1444
BfoI RGCGCY 1 cut(s) 1388
BfrI CTTAAG 1 cut(s) 407
BfuI GTATCC 1 cut(s) 303
BisI GCNGC 7 cut(s) 54, 342, 363, 450, 789, 1271, 1863
BlsI GCNGC 7 cut(s) 55, 343, 364, 451, 790, 1272, 1864
BmcAI AGTACT 2 cut(s) 576, 1013
Bme18I GGWCC 1 cut(s) 1308
BmgT120I GGNCC 3 cut(s) 1308, 1362, 1363
BmiI GGNNCC 4 cut(s) 355, 1091, 1309, 1364
BmrI ACTGGG 1 cut(s) 20
BmsI GCATC 3 cut(s) 901, 1306, 1342
BmuI ACTGGG 1 cut(s) 20
BpiI GAAGAC 2 cut(s) 31, 1196
BpmI CTGGAG 2 cut(s) 259, 813
Bpu10I CCTNAGC 2 cut(s) 297, 1709
Bpu14I TTCGAA 1 cut(s) 1771
BpuEI CTTGAG 2 cut(s) 662, 1333
BsaAI YACGTR 2 cut(s) 246, 326
BsaXI ACNNNNNCTCC 4 cut(s) 232, 262, 689, 719
Bsc4I CCNNNNNNNGG 6 cut(s) 287, 288, 1035, 1595, 1786, 1787
Bse1I ACTGG 3 cut(s) 15, 1641, 1694
Bse3DI GCAATG 3 cut(s) 63, 672, 1292
BseGI GGATG 4 cut(s) 630, 1357, 1525, 1800
BseLI CCNNNNNNNGG 6 cut(s) 287, 288, 1035, 1595, 1786, 1787
BseMI GCAATG 3 cut(s) 63, 672, 1292
BseMII CTCAG 7 cut(s) 88, 179, 585, 1119, 1472, 1653, 1700
BseNI ACTGG 3 cut(s) 15, 1641, 1694
BseRI GAGGAG 2 cut(s) 710, 1581
BseSI GKGCMC 1 cut(s) 1366
BseXI GCAGC 4 cut(s) 40, 353, 775, 1849
BseYI CCCAGC 1 cut(s) 358
BshFI GGCC 5 cut(s) 388, 449, 1364, 1599, 1640
BshNI GGYRCC 1 cut(s) 1089
BsiHKAI GWGCWC 5 cut(s) 245, 280, 1026, 1443, 1479
BslFI GGGAC 2 cut(s) 45, 1321
BslI CCNNNNNNNGG 6 cut(s) 287, 288, 1035, 1595, 1786, 1787
BsmAI GTCTC 4 cut(s) 97, 711, 789, 893
BsmFI GGGAC 2 cut(s) 45, 1321
BsnI GGCC 5 cut(s) 388, 449, 1364, 1599, 1640
Bsp119I TTCGAA 1 cut(s) 1771
Bsp120I GGGCCC 1 cut(s) 1362
Bsp1286I GDGCHC 7 cut(s) 245, 280, 358, 1026, 1366, 1443, 1479
Bsp143I GATC 5 cut(s) 70, 78, 106, 1047, 1096
BspACI CCGC 7 cut(s) 29, 363, 450, 824, 925, 1018, 1271
BspANI GGCC 5 cut(s) 388, 449, 1364, 1599, 1640
BspCNI CTCAG 7 cut(s) 87, 180, 584, 1120, 1471, 1654, 1701
BspLI GGNNCC 4 cut(s) 355, 1091, 1309, 1364
BspMAI CTGCAG 1 cut(s) 1448
BspPI GGATC 1 cut(s) 86
BspQI GCTCTTC 1 cut(s) 1698
BspT104I TTCGAA 1 cut(s) 1771
BspT107I GGYRCC 1 cut(s) 1089
BspTI CTTAAG 1 cut(s) 407
BsrDI GCAATG 3 cut(s) 63, 672, 1292
BsrI ACTGG 3 cut(s) 15, 1641, 1694
BssMI GATC 5 cut(s) 70, 78, 106, 1047, 1096
Bst4CI ACNGT 2 cut(s) 740, 1089
Bst6I CTCTTC 2 cut(s) 545, 1698
BstAFI CTTAAG 1 cut(s) 407
BstBAI YACGTR 2 cut(s) 246, 326
BstBI TTCGAA 1 cut(s) 1771
BstC8I GCNNGC 5 cut(s) 27, 276, 923, 1142, 1685
BstDEI CTNAG 8 cut(s) 74, 188, 297, 571, 1128, 1458, 1662, 1709
BstF5I GGATG 4 cut(s) 630, 1357, 1525, 1800
BstH2I RGCGCY 1 cut(s) 1388
BstHHI GCGC 1 cut(s) 1387
BstKTI GATC 5 cut(s) 73, 81, 109, 1050, 1099
BstMAI GTCTC 4 cut(s) 97, 711, 789, 893
BstMBI GATC 5 cut(s) 70, 78, 106, 1047, 1096
BstMWI GCNNNNNNNGC 4 cut(s) 362, 785, 1361, 1474
BstNSI RCATGY 2 cut(s) 374, 1144
BstSFI CTRYAG 1 cut(s) 1444
BstSLI GKGCMC 1 cut(s) 1366
BstV1I GCAGC 4 cut(s) 40, 353, 775, 1849
BstV2I GAAGAC 2 cut(s) 31, 1196
BstX2I RGATCY 1 cut(s) 78
BstXI CCANNNNNNTGG 1 cut(s) 1843
BstYI RGATCY 1 cut(s) 78
BsuI GTATCC 1 cut(s) 303
BsuRI GGCC 5 cut(s) 388, 449, 1364, 1599, 1640
BtsCI GGATG 4 cut(s) 630, 1357, 1525, 1800
BtsIMutI CAGTG 1 cut(s) 1701
Cac8I GCNNGC 5 cut(s) 27, 276, 923, 1142, 1685
CaiI CAGNNNCTG 2 cut(s) 81, 365
CfoI GCGC 1 cut(s) 1387
Cfr13I GGNCC 3 cut(s) 1308, 1362, 1363
Csp6I GTAC 3 cut(s) 575, 1012, 1090
CviAII CATG 9 cut(s) 261, 371, 758, 827, 1141, 1207, 1782, 1786, 1843
CviQI GTAC 3 cut(s) 575, 1012, 1090
DdeI CTNAG 8 cut(s) 74, 188, 297, 571, 1128, 1458, 1662, 1709
DpnI GATC 5 cut(s) 72, 80, 108, 1049, 1098
DpnII GATC 5 cut(s) 70, 78, 106, 1047, 1096
DraI TTTAAA 1 cut(s) 1452
EaeI YGGCCR 3 cut(s) 447, 1597, 1638
Eam1104I CTCTTC 2 cut(s) 545, 1698
EarI CTCTTC 2 cut(s) 545, 1698
Ecl136II GAGCTC 2 cut(s) 278, 1477
Eco24I GRGCYC 4 cut(s) 280, 358, 1366, 1479
Eco32I GATATC 1 cut(s) 676
Eco47I GGWCC 1 cut(s) 1308
Eco47III AGCGCT 1 cut(s) 1386
Eco53kI GAGCTC 2 cut(s) 278, 1477
Eco72I CACGTG 2 cut(s) 246, 326
EcoICRI GAGCTC 2 cut(s) 278, 1477
EcoO109I RGGNCCY 2 cut(s) 1308, 1362
EcoRI GAATTC 1 cut(s) 1505
EcoRV GATATC 1 cut(s) 676
EcoT22I ATGCAT 4 cut(s) 434, 759, 1142, 1787
EcoT38I GRGCYC 4 cut(s) 280, 358, 1366, 1479
FaeI CATG 9 cut(s) 264, 374, 761, 830, 1144, 1210, 1785, 1789, 1846
FalI AAGNNNNNCTT 2 cut(s) 542, 574
FaqI GGGAC 2 cut(s) 45, 1321
FatI CATG 9 cut(s) 260, 370, 757, 826, 1140, 1206, 1781, 1785, 1842
FauI CCCGC 2 cut(s) 22, 1011
FblI GTMKAC 1 cut(s) 172
Fnu4HI GCNGC 7 cut(s) 54, 342, 363, 450, 789, 1271, 1863
FokI GGATG 4 cut(s) 637, 1364, 1512, 1787
FriOI GRGCYC 4 cut(s) 280, 358, 1366, 1479
Fsp4HI GCNGC 7 cut(s) 54, 342, 363, 450, 789, 1271, 1863
FspBI CTAG 3 cut(s) 314, 1058, 1472
GlaI GCGC 1 cut(s) 1386
GluI GCNGC 7 cut(s) 54, 342, 363, 450, 789, 1271, 1863
GsaI CCCAGC 1 cut(s) 362
GsuI CTGGAG 2 cut(s) 259, 813
HaeII RGCGCY 1 cut(s) 1388
HaeIII GGCC 5 cut(s) 388, 449, 1364, 1599, 1640
HhaI GCGC 1 cut(s) 1387
Hin1II CATG 9 cut(s) 264, 374, 761, 830, 1144, 1210, 1785, 1789, 1846
Hin6I GCGC 1 cut(s) 1385
HinP1I GCGC 1 cut(s) 1385
HincII GTYRAC 1 cut(s) 173
HindII GTYRAC 1 cut(s) 173
HindIII AAGCTT 1 cut(s) 1466
HinfI GANTC 5 cut(s) 257, 847, 1061, 1430, 1581
HphI GGTGA 4 cut(s) 130, 692, 782, 1471
Hpy166II GTNNAC 2 cut(s) 173, 743
Hpy188I TCNGA 9 cut(s) 97, 189, 858, 910, 991, 1435, 1461, 1540, 1821
Hpy188III TCNNGA 5 cut(s) 76, 135, 179, 238, 1472
Hpy8I GTNNAC 2 cut(s) 173, 743
HpyCH4III ACNGT 2 cut(s) 740, 1089
HpyCH4IV ACGT 6 cut(s) 141, 245, 325, 1160, 1228, 1547
HpyF10VI GCNNNNNNNGC 4 cut(s) 362, 785, 1361, 1474
HpyF3I CTNAG 8 cut(s) 74, 188, 297, 571, 1128, 1458, 1662, 1709
HpySE526I ACGT 6 cut(s) 141, 245, 325, 1160, 1228, 1547
Hsp92II CATG 9 cut(s) 264, 374, 761, 830, 1144, 1210, 1785, 1789, 1846
HspAI GCGC 1 cut(s) 1385
KpnI GGTACC 1 cut(s) 1093
Kzo9I GATC 5 cut(s) 70, 78, 106, 1047, 1096
LguI GCTCTTC 1 cut(s) 1698
LmnI GCTCC 6 cut(s) 240, 283, 293, 353, 656, 697
Lsp1109I GCAGC 4 cut(s) 40, 353, 775, 1849
LweI GCATC 3 cut(s) 901, 1306, 1342
MaeI CTAG 3 cut(s) 314, 1058, 1472
MaeII ACGT 6 cut(s) 141, 245, 325, 1160, 1228, 1547
MaeIII GTNAC 7 cut(s) 118, 142, 316, 680, 999, 1490, 1557
MalI GATC 5 cut(s) 72, 80, 108, 1049, 1098
MboI GATC 5 cut(s) 70, 78, 106, 1047, 1096
MfeI CAATTG 1 cut(s) 1004
MflI RGATCY 1 cut(s) 78
MhlI GDGCHC 7 cut(s) 245, 280, 358, 1026, 1366, 1443, 1479
MlsI TGGCCA 2 cut(s) 1599, 1640
MluNI TGGCCA 2 cut(s) 1599, 1640
MlyI GAGTC 2 cut(s) 841, 1439
MmeI TCCRAC 3 cut(s) 866, 1413, 1680
Mox20I TGGCCA 2 cut(s) 1599, 1640
Mph1103I ATGCAT 4 cut(s) 434, 759, 1142, 1787
MroXI GAANNNNTTC 2 cut(s) 130, 1767
MscI TGGCCA 2 cut(s) 1599, 1640
MseI TTAA 6 cut(s) 408, 611, 1133, 1163, 1280, 1451
Msp20I TGGCCA 2 cut(s) 1599, 1640
MspA1I CMGCKG 1 cut(s) 365
MspCI CTTAAG 1 cut(s) 407
MunI CAATTG 1 cut(s) 1004
MwoI GCNNNNNNNGC 4 cut(s) 362, 785, 1361, 1474
NdeII GATC 5 cut(s) 70, 78, 106, 1047, 1096
NlaIII CATG 9 cut(s) 264, 374, 761, 830, 1144, 1210, 1785, 1789, 1846
NlaIV GGNNCC 4 cut(s) 355, 1091, 1309, 1364
NmuCI GTSAC 5 cut(s) 118, 142, 316, 680, 999
NsiI ATGCAT 4 cut(s) 434, 759, 1142, 1787
NspI RCATGY 2 cut(s) 374, 1144
NspV TTCGAA 1 cut(s) 1771
PaeI GCATGC 1 cut(s) 1144
PciI ACATGT 1 cut(s) 370
PciSI GCTCTTC 1 cut(s) 1698
PdmI GAANNNNTTC 2 cut(s) 130, 1767
PfeI GAWTC 3 cut(s) 257, 1061, 1581
PkrI GCNGC 7 cut(s) 55, 343, 364, 451, 790, 1272, 1864
PleI GAGTC 2 cut(s) 841, 1438
PmaCI CACGTG 2 cut(s) 246, 326
PmlI CACGTG 2 cut(s) 246, 326
PpsI GAGTC 2 cut(s) 841, 1438
Ppu21I YACGTR 2 cut(s) 246, 326
PpuMI RGGWCCY 1 cut(s) 1308
PscI ACATGT 1 cut(s) 370
PshBI ATTAAT 1 cut(s) 611
PsiI TTATAA 1 cut(s) 528
Psp124BI GAGCTC 2 cut(s) 280, 1479
Psp5II RGGWCCY 1 cut(s) 1308
PspCI CACGTG 2 cut(s) 246, 326
PspFI CCCAGC 1 cut(s) 358
PspN4I GGNNCC 4 cut(s) 355, 1091, 1309, 1364
PspOMI GGGCCC 1 cut(s) 1362
PspPI GGNCC 3 cut(s) 1308, 1362, 1363
PspPPI RGGWCCY 1 cut(s) 1308
PstI CTGCAG 1 cut(s) 1448
PstNI CAGNNNCTG 2 cut(s) 81, 365
PsuI RGATCY 1 cut(s) 78
RsaI GTAC 3 cut(s) 576, 1013, 1091
RsaNI GTAC 3 cut(s) 575, 1012, 1090
SacI GAGCTC 2 cut(s) 280, 1479
SalI GTCGAC 1 cut(s) 171
SapI GCTCTTC 1 cut(s) 1698
SaqAI TTAA 6 cut(s) 408, 611, 1133, 1163, 1280, 1451
SatI GCNGC 7 cut(s) 54, 342, 363, 450, 789, 1271, 1863
Sau3AI GATC 5 cut(s) 70, 78, 106, 1047, 1096
Sau96I GGNCC 3 cut(s) 1308, 1362, 1363
ScaI AGTACT 2 cut(s) 576, 1013
SchI GAGTC 2 cut(s) 841, 1439
SduI GDGCHC 7 cut(s) 245, 280, 358, 1026, 1366, 1443, 1479
SfaNI GCATC 3 cut(s) 901, 1306, 1342
SfcI CTRYAG 1 cut(s) 1444
SfuI TTCGAA 1 cut(s) 1771
SinI GGWCC 1 cut(s) 1308
SmlI CTYRAG 3 cut(s) 407, 641, 1312
SmoI CTYRAG 3 cut(s) 407, 641, 1312
SpeI ACTAGT 1 cut(s) 313
SphI GCATGC 1 cut(s) 1144
SsiI CCGC 7 cut(s) 29, 363, 450, 824, 925, 1018, 1271
SspI AATATT 2 cut(s) 689, 1167
SspMI CTAG 3 cut(s) 314, 1058, 1472
SstI GAGCTC 2 cut(s) 280, 1479
TaaI ACNGT 2 cut(s) 740, 1089
TaiI ACGT 6 cut(s) 144, 248, 328, 1163, 1231, 1550
TaqI TCGA 7 cut(s) 105, 172, 879, 1050, 1579, 1716, 1771
TaqII GACCGA 1 cut(s) 699
TatI WGTACW 2 cut(s) 574, 1011
TauI GCSGC 3 cut(s) 365, 452, 1273
TfiI GAWTC 3 cut(s) 257, 1061, 1581
Tru1I TTAA 6 cut(s) 408, 611, 1133, 1163, 1280, 1451
Tru9I TTAA 6 cut(s) 408, 611, 1133, 1163, 1280, 1451
TscAI CASTG 1 cut(s) 1701
TseFI GTSAC 5 cut(s) 118, 142, 316, 680, 999
TseI GCWGC 4 cut(s) 53, 341, 788, 1862
Tsp45I GTSAC 5 cut(s) 118, 142, 316, 680, 999
TspDTI ATGAA 8 cut(s) 249, 345, 606, 1388, 1521, 1776, 1789, 1840
TspGWI ACGGA 2 cut(s) 1054, 1793
TspRI CASTG 1 cut(s) 1701
Vha464I CTTAAG 1 cut(s) 407
VpaK11BI GGWCC 1 cut(s) 1308
VspI ATTAAT 1 cut(s) 611
XapI RAATTY 6 cut(s) 394, 593, 724, 1505, 1767, 1773
XbaI TCTAGA 1 cut(s) 1471
XceI RCATGY 2 cut(s) 374, 1144
XmiI GTMKAC 1 cut(s) 172
XmnI GAANNNNTTC 2 cut(s) 130, 1767
XspI CTAG 3 cut(s) 314, 1058, 1472
ZrmI AGTACT 2 cut(s) 576, 1013
Zsp2I ATGCAT 4 cut(s) 434, 759, 1142, 1787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.