RLG00000033846

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002479
Physical Location & Seq
Forward (+)
32907673 .. 32911558
3886 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033846

Sequence Viewer

Length: 840 bp
ATGAGATTGAGATCAAAGACTACAGAGGCTAAACCCTCGCTGGGTTTAGATTTTCGGCTTCCAGCCCCAAATCCAAAGCTCACTCTCGGGTTTGGTGAGGTTGATTATATCAGCAAAATTTGGATGGAGTGCGTGGTTCAGGGAATCATAGAAACACAGCATGTTGAAGCCCTTGAGATTCTTCTTCAGGGTCTTTGTGGTGTTCGCAGAGAACCTTTGAAGGTCCATGAGATATGCCTTAAAAGTGGCCCAAACTTAGGAGCTGTACCTTCTGAGGTTCGACTTATTTGCAATCTTGAACAAACTGAACCTACATGGACTGTGAGGTTCGTGGGGGGTGCGATGAGAGGTGCAGGTGCTGAGCAACTCTCAGTTTTAGTAAGAACTGTGATCGAAAGCAAAGCAAGCAAGAATGTGCTTCGCTTATTTTATGCACTTGGCTACAAGTTGGATCATGAGTTACTGAGGGTTGGGTTTGCGTTCCATTTCCAGAGAGGCACGCAGATGACTGTGACTGTGTCATCAGTTAATAAGATGCCACAACTACACAATACAGATGAAGCTGTCCCTGTAACTCCCGGGATACAGTTGGTTGAAGTTACAGCCCCTGCATCATCTGAAACTTATACTGAAGTTGCGTCGGCAGTGTCATCATTTTGCGAATATCTTGCACCCCTCCTGCATTTGTCAAAACCGGGAATTTCAACTGGGGTTGTTCCTACTGCTGCTGCCGCTGCTGCATCTCTCATGTCTGATGGTGGGGGCGATGAGTTGGTTCCTGGAGAAGCATCCCAGAGTTTCAAGGCCTGGAATGAAGGAGATAGACTTGTTTACGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000414 GO:0000428 GO:0001101 GO:0002831 GO:0003002 GO:0003006 GO:0003674 GO:0003712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0006139 GO:0006351 GO:0006353 GO:0006355 GO:0006366 GO:0006369 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009966 GO:0009987 GO:0010033 GO:0010219 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016591 GO:0016592 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0023051 GO:0030880 GO:0031047 GO:0031050 GO:0031056 GO:0031060 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031399 GO:0031554 GO:0031974 GO:0031981 GO:0032101 GO:0032268 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032991 GO:0033043 GO:0033044 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0040034 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043244 GO:0043254 GO:0043331 GO:0043900 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045892 GO:0045934 GO:0046483 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048441 GO:0048442 GO:0048443 GO:0048464 GO:0048465 GO:0048466 GO:0048467 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048608 GO:0048731 GO:0048827 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051239 GO:0051246 GO:0051252 GO:0051253 GO:0051716 GO:0055029 GO:0060255 GO:0061458 GO:0061695 GO:0065007 GO:0070013 GO:0070847 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090567 GO:0090575 GO:0097305 GO:0097659 GO:0099402 GO:0140110 GO:1900150 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1902275 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000026 GO:2000028 GO:2000031 GO:2000112 GO:2000113 GO:2000142 GO:2000241 GO:2001023 GO:2001038 GO:2001141 GO:2001253
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

280

Amino Acids

30.12

Weight (kDa)

5.91

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 344
Acc36I ACCTGC 1 cut(s) 344
AciI CCGC 1 cut(s) 732
AclWI GGATC 1 cut(s) 459
AcsI RAATTY 2 cut(s) 117, 699
AcuI CTGAAG 2 cut(s) 170, 651
AfaI GTAC 1 cut(s) 267
AfiI CCNNNNNNNGG 2 cut(s) 41, 257
AgsI TTSAA 6 cut(s) 167, 220, 299, 596, 705, 802
AjnI CCWGG 2 cut(s) 778, 806
AluBI AGCT 3 cut(s) 79, 263, 563
AluI AGCT 3 cut(s) 79, 263, 563
AlwI GGATC 1 cut(s) 459
AlwNI CAGNNNCTG 2 cut(s) 359, 608
Ama87I CYCGRG 2 cut(s) 86, 578
AoxI GGCC 2 cut(s) 247, 804
ApeKI GCWGC 4 cut(s) 725, 728, 734, 737
ApoI RAATTY 2 cut(s) 117, 699
AspS9I GGNCC 2 cut(s) 223, 248
AsuC2I CCSGG 3 cut(s) 579, 580, 696
AsuHPI GGTGA 1 cut(s) 107
AvaI CYCGRG 2 cut(s) 86, 578
AvaII GGWCC 1 cut(s) 223
BbvI GCAGC 4 cut(s) 712, 715, 721, 724
BccI CCATC 2 cut(s) 118, 749
BcgI CGANNNNNNTGC 4 cut(s) 270, 304, 650, 684
BciT130I CCWGG 2 cut(s) 780, 808
BciVI GTATCC 1 cut(s) 576
BcnI CCSGG 3 cut(s) 579, 580, 696
BfmI CTRYAG 1 cut(s) 21
BfuAI ACCTGC 1 cut(s) 344
BfuI GTATCC 1 cut(s) 576
BisI GCNGC 5 cut(s) 726, 729, 732, 735, 738
BlpI GCTNAGC 1 cut(s) 360
BlsI GCNGC 5 cut(s) 727, 730, 733, 736, 739
Bme1390I CCNGG 5 cut(s) 579, 580, 696, 780, 808
Bme18I GGWCC 1 cut(s) 223
BmeT110I CYCGRG 2 cut(s) 86, 578
BmgT120I GGNCC 2 cut(s) 223, 248
BmiI GGNNCC 1 cut(s) 777
BmrFI CCNGG 5 cut(s) 579, 580, 696, 780, 808
BmrI ACTGGG 1 cut(s) 717
BmsI GCATC 4 cut(s) 525, 620, 749, 797
BmuI ACTGGG 1 cut(s) 717
BplI GAGNNNNNCTC 2 cut(s) 353, 385
BpmI CTGGAG 1 cut(s) 801
Bpu1102I GCTNAGC 1 cut(s) 360
BpuEI CTTGAG 1 cut(s) 194
BpuMI CCSGG 3 cut(s) 579, 580, 696
BsaBI GATNNNNATC 1 cut(s) 10
BsaJI CCNNGG 1 cut(s) 578
Bsc4I CCNNNNNNNGG 2 cut(s) 41, 257
Bse1I ACTGG 1 cut(s) 712
Bse8I GATNNNNATC 1 cut(s) 10
BseBI CCWGG 2 cut(s) 780, 808
BseDI CCNNGG 1 cut(s) 578
BseGI GGATG 2 cut(s) 129, 788
BseJI GATNNNNATC 1 cut(s) 10
BseLI CCNNNNNNNGG 2 cut(s) 41, 257
BseMII CTCAG 4 cut(s) 264, 351, 384, 455
BseNI ACTGG 1 cut(s) 712
BseXI GCAGC 4 cut(s) 712, 715, 721, 724
BseYI CCCAGC 1 cut(s) 40
BsgI GTGCAG 1 cut(s) 372
BshFI GGCC 2 cut(s) 249, 806
BsiHKCI CYCGRG 2 cut(s) 86, 578
BsiSI CCGG 2 cut(s) 579, 695
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 2 cut(s) 41, 257
BsmFI GGGAC 1 cut(s) 551
BsnI GGCC 2 cut(s) 249, 806
BsoBI CYCGRG 2 cut(s) 86, 578
Bsp143I GATC 3 cut(s) 11, 390, 451
Bsp1720I GCTNAGC 1 cut(s) 360
BspACI CCGC 1 cut(s) 732
BspANI GGCC 2 cut(s) 249, 806
BspCNI CTCAG 4 cut(s) 265, 352, 383, 456
BspHI TCATGA 1 cut(s) 454
BspLI GGNNCC 1 cut(s) 777
BspMI ACCTGC 1 cut(s) 344
BspPI GGATC 1 cut(s) 459
BsrI ACTGG 1 cut(s) 712
BssECI CCNNGG 1 cut(s) 578
BssMI GATC 3 cut(s) 11, 390, 451
Bst2UI CCWGG 2 cut(s) 780, 808
Bst4CI ACNGT 5 cut(s) 322, 388, 511, 517, 588
BstC8I GCNNGC 2 cut(s) 406, 500
BstDEI CTNAG 5 cut(s) 256, 273, 360, 370, 464
BstF5I GGATG 2 cut(s) 129, 788
BstKTI GATC 3 cut(s) 14, 393, 454
BstMBI GATC 3 cut(s) 11, 390, 451
BstMWI GCNNNNNNNGC 4 cut(s) 405, 731, 734, 737
BstNI CCWGG 2 cut(s) 780, 808
BstNSI RCATGY 1 cut(s) 164
BstSCI CCNGG 5 cut(s) 577, 578, 694, 778, 806
BstSFI CTRYAG 1 cut(s) 21
BstV1I GCAGC 4 cut(s) 712, 715, 721, 724
BsuI GTATCC 1 cut(s) 576
BsuRI GGCC 2 cut(s) 249, 806
BtgZI GCGATG 2 cut(s) 356, 780
BtsCI GGATG 2 cut(s) 129, 788
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 1 cut(s) 651
BveI ACCTGC 1 cut(s) 344
Cac8I GCNNGC 2 cut(s) 406, 500
CaiI CAGNNNCTG 2 cut(s) 359, 608
CciI TCATGA 1 cut(s) 454
Cfr13I GGNCC 2 cut(s) 223, 248
Cfr9I CCCGGG 1 cut(s) 578
CseI GACGC 1 cut(s) 627
Csp6I GTAC 1 cut(s) 266
CviAII CATG 5 cut(s) 161, 227, 315, 455, 748
CviQI GTAC 1 cut(s) 266
DdeI CTNAG 5 cut(s) 256, 273, 360, 370, 464
DpnI GATC 3 cut(s) 13, 392, 453
DpnII GATC 3 cut(s) 11, 390, 451
Eco147I AGGCCT 1 cut(s) 806
Eco47I GGWCC 1 cut(s) 223
Eco57I CTGAAG 2 cut(s) 170, 651
Eco88I CYCGRG 2 cut(s) 86, 578
EcoRII CCWGG 2 cut(s) 778, 806
FaeI CATG 5 cut(s) 164, 230, 318, 458, 751
FaqI GGGAC 1 cut(s) 551
FatI CATG 5 cut(s) 160, 226, 314, 454, 747
Fnu4HI GCNGC 5 cut(s) 726, 729, 732, 735, 738
FokI GGATG 2 cut(s) 136, 775
Fsp4HI GCNGC 5 cut(s) 726, 729, 732, 735, 738
GluI GCNGC 5 cut(s) 726, 729, 732, 735, 738
GsaI CCCAGC 1 cut(s) 44
GsuI CTGGAG 1 cut(s) 801
HaeIII GGCC 2 cut(s) 249, 806
HapII CCGG 2 cut(s) 579, 695
HgaI GACGC 1 cut(s) 627
Hin1II CATG 5 cut(s) 164, 230, 318, 458, 751
HinfI GANTC 2 cut(s) 144, 178
HpaII CCGG 2 cut(s) 579, 695
HphI GGTGA 1 cut(s) 107
Hpy166II GTNNAC 1 cut(s) 832
Hpy188I TCNGA 3 cut(s) 274, 619, 754
Hpy188III TCNNGA 3 cut(s) 296, 455, 490
Hpy8I GTNNAC 1 cut(s) 832
Hpy99I CGWCG 1 cut(s) 643
HpyAV CCTTC 3 cut(s) 214, 279, 809
HpyCH4III ACNGT 5 cut(s) 322, 388, 511, 517, 588
HpyCH4V TGCA 7 cut(s) 291, 353, 434, 611, 671, 682, 740
HpyF10VI GCNNNNNNNGC 4 cut(s) 405, 731, 734, 737
HpyF3I CTNAG 5 cut(s) 256, 273, 360, 370, 464
Hsp92II CATG 5 cut(s) 164, 230, 318, 458, 751
Kzo9I GATC 3 cut(s) 11, 390, 451
LmnI GCTCC 1 cut(s) 260
Lsp1109I GCAGC 4 cut(s) 712, 715, 721, 724
LweI GCATC 4 cut(s) 525, 620, 749, 797
MaeIII GTNAC 4 cut(s) 459, 511, 571, 598
MalI GATC 3 cut(s) 13, 392, 453
MboI GATC 3 cut(s) 11, 390, 451
MboII GAAGA 2 cut(s) 173, 176
MluCI AATT 2 cut(s) 117, 699
MmeI TCCRAC 1 cut(s) 429
MnlI CCTC 9 cut(s) 19, 46, 91, 268, 318, 341, 459, 488, 686
MseI TTAA 2 cut(s) 240, 528
MslI CAYNNNNRTG 1 cut(s) 503
MspA1I CMGCKG 1 cut(s) 734
MspI CCGG 2 cut(s) 579, 695
MspR9I CCNGG 5 cut(s) 579, 580, 696, 780, 808
MvaI CCWGG 2 cut(s) 780, 808
MwoI GCNNNNNNNGC 4 cut(s) 405, 731, 734, 737
NciI CCSGG 3 cut(s) 579, 580, 696
NdeII GATC 3 cut(s) 11, 390, 451
NlaIII CATG 5 cut(s) 164, 230, 318, 458, 751
NlaIV GGNNCC 1 cut(s) 777
NmuCI GTSAC 1 cut(s) 511
NspI RCATGY 1 cut(s) 164
PagI TCATGA 1 cut(s) 454
PaqCI CACCTGC 1 cut(s) 344
PceI AGGCCT 1 cut(s) 806
PfeI GAWTC 2 cut(s) 144, 178
PflFI GACNNNGTC 1 cut(s) 517
PfoI TCCNGGA 1 cut(s) 778
PkrI GCNGC 5 cut(s) 727, 730, 733, 736, 739
Psp6I CCWGG 2 cut(s) 778, 806
PspFI CCCAGC 1 cut(s) 40
PspGI CCWGG 2 cut(s) 778, 806
PspN4I GGNNCC 1 cut(s) 777
PspPI GGNCC 2 cut(s) 223, 248
PstNI CAGNNNCTG 2 cut(s) 359, 608
PsyI GACNNNGTC 1 cut(s) 517
RsaI GTAC 1 cut(s) 267
RsaNI GTAC 1 cut(s) 266
RseI CAYNNNNRTG 1 cut(s) 503
SaqAI TTAA 2 cut(s) 240, 528
SatI GCNGC 5 cut(s) 726, 729, 732, 735, 738
Sau3AI GATC 3 cut(s) 11, 390, 451
Sau96I GGNCC 2 cut(s) 223, 248
ScrFI CCNGG 5 cut(s) 579, 580, 696, 780, 808
SfaNI GCATC 4 cut(s) 525, 620, 749, 797
SfcI CTRYAG 1 cut(s) 21
SinI GGWCC 1 cut(s) 223
SmaI CCCGGG 1 cut(s) 580
SmiMI CAYNNNNRTG 1 cut(s) 503
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
Sse9I AATT 2 cut(s) 117, 699
SseBI AGGCCT 1 cut(s) 806
SsiI CCGC 1 cut(s) 732
StuI AGGCCT 1 cut(s) 806
StyD4I CCNGG 5 cut(s) 577, 578, 694, 778, 806
TaaI ACNGT 5 cut(s) 322, 388, 511, 517, 588
TaqI TCGA 2 cut(s) 280, 393
TasI AATT 2 cut(s) 117, 699
TauI GCSGC 1 cut(s) 734
TfiI GAWTC 2 cut(s) 144, 178
Tru1I TTAA 2 cut(s) 240, 528
Tru9I TTAA 2 cut(s) 240, 528
TscAI CASTG 1 cut(s) 651
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 4 cut(s) 725, 728, 734, 737
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 2 cut(s) 573, 828
TspMI CCCGGG 1 cut(s) 578
TspRI CASTG 1 cut(s) 651
Tth111I GACNNNGTC 1 cut(s) 517
VpaK11BI GGWCC 1 cut(s) 223
XapI RAATTY 2 cut(s) 117, 699
XceI RCATGY 1 cut(s) 164
XmaI CCCGGG 1 cut(s) 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.