Rw5G021760

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002479
Physical Location & Seq
Reverse (-)
29575816 .. 29577710
1895 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G021760.1

Sequence Viewer

Length: 654 bp
ATGGAGTGCGTGGTTCAGGGAATCATAGAAACACAGCATGTTGAAGCCCTTGAGATTCTTCTTCAGGGTCTTTGTGGTGTTCGCAGAGAACCTTTGAAGGTCCATGAGATATGCCTGAAAAGTGGCCCAAACTTAGGAGCTGTACCTTCTGAGGTTCGACTTATTTGCAATCTTGAACAAACTGAACCTACATGGACTGTGAGGTTCGTGGGGGGTGCGATGAGAGGTGCAGGTGCTGAGCAACTCTCAGTTTTAGTAAGAACTGTGATCGAAAGCAAAGCAAGCAAGAATGTGCTTCGCTTATTTTATGCACTTGGCTACAAGTTGGATCATGAGTTACTGAGGGTCGGGTTTGCGTTCCATTTCCAGAGAGGCACGCAGATGACTGTGACTGTGTCATCAGTTAATAAGATGCCACAACTACACAATACAGATGAAGCTGTCCCTGTAACTCCCGGGATACAGTTGGTTGAAGTTACAGCCCCTGCATTATCTGAAACTTATACTGAAGTTGCGTCGGCAGTGTCATCATTTTGCGAATATCTTGCACCCCTCCTGCATTTGTCAAAACCGGGAATTTCAACTGGGGTTGTTCCTACTGCTGCTGCCGCTGCTGCATCTCTCATGTCTGATGGTGGGGGTACTACCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000414 GO:0000428 GO:0001101 GO:0002831 GO:0003002 GO:0003006 GO:0003674 GO:0003712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0006139 GO:0006351 GO:0006353 GO:0006355 GO:0006366 GO:0006369 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009966 GO:0009987 GO:0010033 GO:0010219 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016591 GO:0016592 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0023051 GO:0030880 GO:0031047 GO:0031050 GO:0031056 GO:0031060 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031399 GO:0031554 GO:0031974 GO:0031981 GO:0032101 GO:0032268 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032991 GO:0033043 GO:0033044 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0040034 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043244 GO:0043254 GO:0043331 GO:0043900 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045892 GO:0045934 GO:0046483 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048441 GO:0048442 GO:0048443 GO:0048464 GO:0048465 GO:0048466 GO:0048467 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048608 GO:0048731 GO:0048827 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051172 GO:0051239 GO:0051246 GO:0051252 GO:0051253 GO:0051716 GO:0055029 GO:0060255 GO:0061458 GO:0061695 GO:0065007 GO:0070013 GO:0070847 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090567 GO:0090575 GO:0097305 GO:0097659 GO:0099402 GO:0140110 GO:1900150 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1902275 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000026 GO:2000028 GO:2000031 GO:2000112 GO:2000113 GO:2000142 GO:2000241 GO:2001023 GO:2001038 GO:2001141 GO:2001253
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

217

Amino Acids

23.17

Weight (kDa)

5.87

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 221
Acc36I ACCTGC 1 cut(s) 221
AciI CCGC 1 cut(s) 609
AclWI GGATC 1 cut(s) 336
AcsI RAATTY 1 cut(s) 576
AcuI CTGAAG 2 cut(s) 47, 528
AfaI GTAC 2 cut(s) 144, 643
AfiI CCNNNNNNNGG 1 cut(s) 134
AgsI TTSAA 5 cut(s) 44, 97, 176, 473, 582
AluBI AGCT 2 cut(s) 140, 440
AluI AGCT 2 cut(s) 140, 440
AlwI GGATC 1 cut(s) 336
AlwNI CAGNNNCTG 2 cut(s) 236, 485
Ama87I CYCGRG 1 cut(s) 455
AoxI GGCC 1 cut(s) 124
ApeKI GCWGC 4 cut(s) 602, 605, 611, 614
ApoI RAATTY 1 cut(s) 576
AspS9I GGNCC 2 cut(s) 100, 125
AsuC2I CCSGG 3 cut(s) 456, 457, 573
AvaI CYCGRG 1 cut(s) 455
AvaII GGWCC 1 cut(s) 100
BbvI GCAGC 4 cut(s) 589, 592, 598, 601
BccI CCATC 1 cut(s) 626
BcgI CGANNNNNNTGC 4 cut(s) 147, 181, 527, 561
BciVI GTATCC 1 cut(s) 453
BcnI CCSGG 3 cut(s) 456, 457, 573
BfuAI ACCTGC 1 cut(s) 221
BfuI GTATCC 1 cut(s) 453
BisI GCNGC 5 cut(s) 603, 606, 609, 612, 615
BlpI GCTNAGC 1 cut(s) 237
BlsI GCNGC 5 cut(s) 604, 607, 610, 613, 616
Bme1390I CCNGG 3 cut(s) 456, 457, 573
Bme18I GGWCC 1 cut(s) 100
BmeT110I CYCGRG 1 cut(s) 455
BmgT120I GGNCC 2 cut(s) 100, 125
BmrFI CCNGG 3 cut(s) 456, 457, 573
BmrI ACTGGG 1 cut(s) 594
BmsI GCATC 2 cut(s) 402, 626
BmuI ACTGGG 1 cut(s) 594
BplI GAGNNNNNCTC 2 cut(s) 230, 262
Bpu1102I GCTNAGC 1 cut(s) 237
BpuEI CTTGAG 1 cut(s) 71
BpuMI CCSGG 3 cut(s) 456, 457, 573
BsaJI CCNNGG 1 cut(s) 455
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse1I ACTGG 1 cut(s) 589
BseDI CCNNGG 1 cut(s) 455
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMII CTCAG 4 cut(s) 141, 228, 261, 332
BseNI ACTGG 1 cut(s) 589
BseXI GCAGC 4 cut(s) 589, 592, 598, 601
BsgI GTGCAG 1 cut(s) 249
BshFI GGCC 1 cut(s) 126
BsiHKCI CYCGRG 1 cut(s) 455
BsiSI CCGG 2 cut(s) 456, 572
BslFI GGGAC 1 cut(s) 428
BslI CCNNNNNNNGG 1 cut(s) 134
BsmFI GGGAC 1 cut(s) 428
BsnI GGCC 1 cut(s) 126
BsoBI CYCGRG 1 cut(s) 455
Bsp143I GATC 2 cut(s) 267, 328
Bsp1720I GCTNAGC 1 cut(s) 237
BspACI CCGC 1 cut(s) 609
BspANI GGCC 1 cut(s) 126
BspCNI CTCAG 4 cut(s) 142, 229, 260, 333
BspHI TCATGA 1 cut(s) 331
BspMI ACCTGC 1 cut(s) 221
BspPI GGATC 1 cut(s) 336
BsrI ACTGG 1 cut(s) 589
BssECI CCNNGG 1 cut(s) 455
BssMI GATC 2 cut(s) 267, 328
Bst4CI ACNGT 5 cut(s) 199, 265, 388, 394, 465
BstC8I GCNNGC 2 cut(s) 283, 377
BstDEI CTNAG 5 cut(s) 133, 150, 237, 247, 341
BstKTI GATC 2 cut(s) 270, 331
BstMBI GATC 2 cut(s) 267, 328
BstMWI GCNNNNNNNGC 4 cut(s) 282, 608, 611, 614
BstNSI RCATGY 1 cut(s) 41
BstSCI CCNGG 3 cut(s) 454, 455, 571
BstV1I GCAGC 4 cut(s) 589, 592, 598, 601
BsuI GTATCC 1 cut(s) 453
BsuRI GGCC 1 cut(s) 126
BtgZI GCGATG 1 cut(s) 233
BtsI GCAGTG 1 cut(s) 528
BtsIMutI CAGTG 1 cut(s) 528
BveI ACCTGC 1 cut(s) 221
Cac8I GCNNGC 2 cut(s) 283, 377
CaiI CAGNNNCTG 2 cut(s) 236, 485
CciI TCATGA 1 cut(s) 331
Cfr13I GGNCC 2 cut(s) 100, 125
Cfr9I CCCGGG 1 cut(s) 455
CseI GACGC 1 cut(s) 504
Csp6I GTAC 2 cut(s) 143, 642
CviAII CATG 5 cut(s) 38, 104, 192, 332, 625
CviJI RGCY 6 cut(s) 47, 126, 140, 318, 440, 482
CviKI_1 RGCY 6 cut(s) 47, 126, 140, 318, 440, 482
CviQI GTAC 2 cut(s) 143, 642
DdeI CTNAG 5 cut(s) 133, 150, 237, 247, 341
DpnI GATC 2 cut(s) 269, 330
DpnII GATC 2 cut(s) 267, 328
Eco47I GGWCC 1 cut(s) 100
Eco57I CTGAAG 2 cut(s) 47, 528
Eco88I CYCGRG 1 cut(s) 455
FaeI CATG 5 cut(s) 41, 107, 195, 335, 628
FaqI GGGAC 1 cut(s) 428
FatI CATG 5 cut(s) 37, 103, 191, 331, 624
Fnu4HI GCNGC 5 cut(s) 603, 606, 609, 612, 615
Fsp4HI GCNGC 5 cut(s) 603, 606, 609, 612, 615
GluI GCNGC 5 cut(s) 603, 606, 609, 612, 615
HaeIII GGCC 1 cut(s) 126
HapII CCGG 2 cut(s) 456, 572
HgaI GACGC 1 cut(s) 504
Hin1II CATG 5 cut(s) 41, 107, 195, 335, 628
HinfI GANTC 2 cut(s) 21, 55
HpaII CCGG 2 cut(s) 456, 572
Hpy188I TCNGA 3 cut(s) 151, 496, 631
Hpy188III TCNNGA 3 cut(s) 173, 332, 367
Hpy99I CGWCG 1 cut(s) 520
HpyAV CCTTC 2 cut(s) 91, 156
HpyCH4III ACNGT 5 cut(s) 199, 265, 388, 394, 465
HpyCH4V TGCA 7 cut(s) 168, 230, 311, 488, 548, 559, 617
HpyF10VI GCNNNNNNNGC 4 cut(s) 282, 608, 611, 614
HpyF3I CTNAG 5 cut(s) 133, 150, 237, 247, 341
Hsp92II CATG 5 cut(s) 41, 107, 195, 335, 628
Kzo9I GATC 2 cut(s) 267, 328
LmnI GCTCC 1 cut(s) 137
Lsp1109I GCAGC 4 cut(s) 589, 592, 598, 601
LweI GCATC 2 cut(s) 402, 626
MaeIII GTNAC 4 cut(s) 336, 388, 448, 475
MalI GATC 2 cut(s) 269, 330
MboI GATC 2 cut(s) 267, 328
MboII GAAGA 2 cut(s) 50, 53
MluCI AATT 1 cut(s) 576
MmeI TCCRAC 1 cut(s) 306
MnlI CCTC 6 cut(s) 145, 195, 218, 336, 365, 563
MseI TTAA 1 cut(s) 405
MslI CAYNNNNRTG 1 cut(s) 380
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 2 cut(s) 456, 572
MspR9I CCNGG 3 cut(s) 456, 457, 573
MwoI GCNNNNNNNGC 4 cut(s) 282, 608, 611, 614
NciI CCSGG 3 cut(s) 456, 457, 573
NdeII GATC 2 cut(s) 267, 328
NlaIII CATG 5 cut(s) 41, 107, 195, 335, 628
NmuCI GTSAC 1 cut(s) 388
NspI RCATGY 1 cut(s) 41
PagI TCATGA 1 cut(s) 331
PaqCI CACCTGC 1 cut(s) 221
PfeI GAWTC 2 cut(s) 21, 55
PflFI GACNNNGTC 1 cut(s) 394
PkrI GCNGC 5 cut(s) 604, 607, 610, 613, 616
PspPI GGNCC 2 cut(s) 100, 125
PstNI CAGNNNCTG 2 cut(s) 236, 485
PsyI GACNNNGTC 1 cut(s) 394
RsaI GTAC 2 cut(s) 144, 643
RsaNI GTAC 2 cut(s) 143, 642
RseI CAYNNNNRTG 1 cut(s) 380
SaqAI TTAA 1 cut(s) 405
SatI GCNGC 5 cut(s) 603, 606, 609, 612, 615
Sau3AI GATC 2 cut(s) 267, 328
Sau96I GGNCC 2 cut(s) 100, 125
ScrFI CCNGG 3 cut(s) 456, 457, 573
SfaNI GCATC 2 cut(s) 402, 626
SinI GGWCC 1 cut(s) 100
SmaI CCCGGG 1 cut(s) 457
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 1 cut(s) 576
SsiI CCGC 1 cut(s) 609
StyD4I CCNGG 3 cut(s) 454, 455, 571
TaaI ACNGT 5 cut(s) 199, 265, 388, 394, 465
TaqI TCGA 2 cut(s) 157, 270
TasI AATT 1 cut(s) 576
TauI GCSGC 1 cut(s) 611
TfiI GAWTC 2 cut(s) 21, 55
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TscAI CASTG 1 cut(s) 528
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 4 cut(s) 602, 605, 611, 614
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 1 cut(s) 450
TspMI CCCGGG 1 cut(s) 455
TspRI CASTG 1 cut(s) 528
Tth111I GACNNNGTC 1 cut(s) 394
VpaK11BI GGWCC 1 cut(s) 100
XapI RAATTY 1 cut(s) 576
XceI RCATGY 1 cut(s) 41
XmaI CCCGGG 1 cut(s) 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.