AT2G28210

alpha carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006048
Physical Location & Seq
Forward (+)
12029666 .. 12033096
3431 bp
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UTR
Exon/CDS
Intron
AT2G28210.1

Sequence Viewer

Length: 831 bp
ATGGATAAAATATCAATCCGATGCTTTATCTTCTTAGTTCTCACCAGCTTCGTCACCACTGTTTCGTGTTTGAGTGCAGCGACAGATTATAGAGAAGTTGAGGATGAACACGAATTCAGCTACGAATGGAACCAAGAGAACGGGCCAGCGAAATGGGGAAAGCTAAGACCGGAATGGAAAATGTGCGGAAAAGGAGAAATGCAATCGCCTATTGATCTTATGAACAAAAGAGTTAGACTTGTTACTCATCTTAAAAAGCTTACTAGACACTACAAACCTTGTAACGCCACTCTCAAAAATAGAGGCCATGATATGATGCTGAAATTTGGAGAAGAAGGGTCAGGGAGTATTACGGTCAATGGAACTGAGTATAAACTCTTACAGCTTCATTGGCATTCTCCCTCTGAACATACTATGAATGGAAGAAGGTTTGCTCTCGAGCTACACATGGTTCACGAAAACATTAACGGAAGTTTGGCTGTAGTCACAGTCCTCTACAAAATCGGAAGGCCAGATTCTTTTCTCGGATTGCTGGAAAATAAATTGTCGGCAATTACAGATCAAAATGAGGCGGAGAAATATGTAGATGTGATTGACCCAAGGGATATTAAGATTGGGAGCAGAAAATTTTATAGATACATTGGATCACTTACTACTCCTCCTTGTACGCAAAATGTTATTTGGACCGTCGTTAAAAAGGTAAGGACTGTGACGAAAAACCAAGTGAAGCTACTCAGAGTGGCGGTTCACGATAATTCAGACACAAATGCGAGACCAGTTCAACCTACAAATAAGCGCGTGGTAAAGTTATACAAACCAAAATCACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

276

Amino Acids

31.72

Weight (kDa)

9.5

Isoelectric Point (pI)

26.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 47 - 269 1e-43 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 798
AciI CCGC 3 cut(s) 186, 572, 743
AclWI GGATC 1 cut(s) 652
AcsI RAATTY 3 cut(s) 113, 323, 626
AfaI GTAC 1 cut(s) 667
AgsI TTSAA 1 cut(s) 782
AluBI AGCT 7 cut(s) 48, 120, 163, 259, 385, 442, 730
AluI AGCT 7 cut(s) 48, 120, 163, 259, 385, 442, 730
Alw26I GTCTC 1 cut(s) 766
AlwI GGATC 1 cut(s) 652
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 3 cut(s) 143, 304, 509
ApeKI GCWGC 1 cut(s) 77
ApoI RAATTY 3 cut(s) 113, 323, 626
AspLEI GCGC 1 cut(s) 798
AspS9I GGNCC 2 cut(s) 143, 684
AsuHPI GGTGA 2 cut(s) 34, 46
AvaI CYCGRG 1 cut(s) 437
AvaII GGWCC 1 cut(s) 684
BbvI GCAGC 1 cut(s) 89
BcoDI GTCTC 1 cut(s) 766
BfaI CTAG 1 cut(s) 264
BfmI CTRYAG 1 cut(s) 480
BisI GCNGC 1 cut(s) 78
BlsI GCNGC 1 cut(s) 79
Bme18I GGWCC 1 cut(s) 684
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 2 cut(s) 143, 684
BmiI GGNNCC 1 cut(s) 131
BmsI GCATC 2 cut(s) 11, 306
BsaI GGTCTC 1 cut(s) 766
BsaJI CCNNGG 1 cut(s) 599
BsaWI WCCGGW 1 cut(s) 169
BsaXI ACNNNNNCTCC 2 cut(s) 643, 673
Bse1I ACTGG 1 cut(s) 776
BseDI CCNNGG 1 cut(s) 599
BseGI GGATG 1 cut(s) 109
BseMII CTCAG 2 cut(s) 357, 748
BseNI ACTGG 1 cut(s) 776
BseRI GAGGAG 1 cut(s) 648
BseXI GCAGC 1 cut(s) 89
BsgI GTGCAG 1 cut(s) 96
Bsh1236I CGCG 1 cut(s) 798
BshFI GGCC 3 cut(s) 145, 306, 511
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 1 cut(s) 170
BsmAI GTCTC 1 cut(s) 766
BsmI GAATGC 1 cut(s) 394
BsnI GGCC 3 cut(s) 145, 306, 511
Bso31I GGTCTC 1 cut(s) 766
BsoBI CYCGRG 1 cut(s) 437
Bsp143I GATC 3 cut(s) 214, 559, 644
BspACI CCGC 3 cut(s) 186, 572, 743
BspANI GGCC 3 cut(s) 145, 306, 511
BspCNI CTCAG 2 cut(s) 358, 747
BspFNI CGCG 1 cut(s) 798
BspLI GGNNCC 1 cut(s) 131
BspPI GGATC 1 cut(s) 652
BspTNI GGTCTC 1 cut(s) 766
BsrI ACTGG 1 cut(s) 776
BssECI CCNNGG 1 cut(s) 599
BssMI GATC 3 cut(s) 214, 559, 644
BssT1I CCWWGG 1 cut(s) 599
Bst4CI ACNGT 5 cut(s) 61, 355, 490, 688, 709
BstC8I GCNNGC 1 cut(s) 147
BstDEI CTNAG 4 cut(s) 34, 164, 366, 734
BstF5I GGATG 1 cut(s) 109
BstFNI CGCG 1 cut(s) 798
BstHHI GCGC 1 cut(s) 798
BstKTI GATC 3 cut(s) 217, 562, 647
BstMAI GTCTC 1 cut(s) 766
BstMBI GATC 3 cut(s) 214, 559, 644
BstMWI GCNNNNNNNGC 1 cut(s) 391
BstSFI CTRYAG 1 cut(s) 480
BstUI CGCG 1 cut(s) 798
BstV1I GCAGC 1 cut(s) 89
BstXI CCANNNNNNTGG 1 cut(s) 153
BsuRI GGCC 3 cut(s) 145, 306, 511
BtsCI GGATG 1 cut(s) 109
BtsIMutI CAGTG 1 cut(s) 57
Cac8I GCNNGC 1 cut(s) 147
CfoI GCGC 1 cut(s) 798
Cfr13I GGNCC 2 cut(s) 143, 684
Csp6I GTAC 1 cut(s) 666
CviAII CATG 2 cut(s) 308, 448
CviQI GTAC 1 cut(s) 666
DdeI CTNAG 4 cut(s) 34, 164, 366, 734
DpnI GATC 3 cut(s) 216, 561, 646
DpnII GATC 3 cut(s) 214, 559, 644
EciI GGCGGA 1 cut(s) 587
Eco130I CCWWGG 1 cut(s) 599
Eco31I GGTCTC 1 cut(s) 766
Eco47I GGWCC 1 cut(s) 684
Eco88I CYCGRG 1 cut(s) 437
EcoRI GAATTC 1 cut(s) 113
EcoT14I CCWWGG 1 cut(s) 599
ErhI CCWWGG 1 cut(s) 599
FaeI CATG 2 cut(s) 311, 451
FatI CATG 2 cut(s) 307, 447
Fnu4HI GCNGC 1 cut(s) 78
FokI GGATG 1 cut(s) 116
Fsp4HI GCNGC 1 cut(s) 78
FspBI CTAG 1 cut(s) 264
GlaI GCGC 1 cut(s) 797
GluI GCNGC 1 cut(s) 78
HaeIII GGCC 3 cut(s) 145, 306, 511
HapII CCGG 1 cut(s) 170
HhaI GCGC 1 cut(s) 798
Hin1II CATG 2 cut(s) 311, 451
Hin6I GCGC 1 cut(s) 796
HinP1I GCGC 1 cut(s) 796
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 1 cut(s) 515
HpaII CCGG 1 cut(s) 170
HphI GGTGA 2 cut(s) 34, 46
Hpy166II GTNNAC 2 cut(s) 454, 748
Hpy188I TCNGA 6 cut(s) 20, 406, 506, 527, 737, 760
Hpy188III TCNNGA 3 cut(s) 437, 455, 749
Hpy8I GTNNAC 2 cut(s) 454, 748
Hpy99I CGWCG 1 cut(s) 692
HpyAV CCTTC 3 cut(s) 329, 420, 501
HpyCH4III ACNGT 5 cut(s) 61, 355, 490, 688, 709
HpyCH4V TGCA 2 cut(s) 77, 202
HpyF10VI GCNNNNNNNGC 1 cut(s) 391
HpyF3I CTNAG 4 cut(s) 34, 164, 366, 734
Hsp92II CATG 2 cut(s) 311, 451
HspAI GCGC 1 cut(s) 796
Kzo9I GATC 3 cut(s) 214, 559, 644
LmnI GCTCC 1 cut(s) 618
LpnPI CCDG 7 cut(s) 58, 159, 183, 327, 518, 525, 789
Lsp1109I GCAGC 1 cut(s) 89
LweI GCATC 2 cut(s) 11, 306
MaeI CTAG 1 cut(s) 264
MaeIII GTNAC 5 cut(s) 52, 241, 281, 484, 709
MalI GATC 3 cut(s) 216, 561, 646
MboI GATC 3 cut(s) 214, 559, 644
MboII GAAGA 3 cut(s) 22, 344, 435
MluCI AATT 6 cut(s) 113, 323, 542, 552, 626, 754
MnlI CCTC 6 cut(s) 94, 296, 412, 503, 562, 669
MseI TTAA 4 cut(s) 252, 465, 609, 693
MspI CCGG 1 cut(s) 170
Mva1269I GAATGC 1 cut(s) 394
MvnI CGCG 1 cut(s) 798
MwoI GCNNNNNNNGC 1 cut(s) 391
NdeII GATC 3 cut(s) 214, 559, 644
NlaIII CATG 2 cut(s) 311, 451
NlaIV GGNNCC 1 cut(s) 131
NmuCI GTSAC 3 cut(s) 52, 484, 709
PaeR7I CTCGAG 1 cut(s) 437
PctI GAATGC 1 cut(s) 394
PfeI GAWTC 1 cut(s) 515
PkrI GCNGC 1 cut(s) 79
PspN4I GGNNCC 1 cut(s) 131
PspPI GGNCC 2 cut(s) 143, 684
RsaI GTAC 1 cut(s) 667
RsaNI GTAC 1 cut(s) 666
SaqAI TTAA 4 cut(s) 252, 465, 609, 693
SatI GCNGC 1 cut(s) 78
Sau3AI GATC 3 cut(s) 214, 559, 644
Sau96I GGNCC 2 cut(s) 143, 684
SfaNI GCATC 2 cut(s) 11, 306
SfcI CTRYAG 1 cut(s) 480
Sfr274I CTCGAG 1 cut(s) 437
SinI GGWCC 1 cut(s) 684
SlaI CTCGAG 1 cut(s) 437
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
Sse9I AATT 6 cut(s) 113, 323, 542, 552, 626, 754
SsiI CCGC 3 cut(s) 186, 572, 743
SspMI CTAG 1 cut(s) 264
StyI CCWWGG 1 cut(s) 599
TaaI ACNGT 5 cut(s) 61, 355, 490, 688, 709
TaqI TCGA 1 cut(s) 438
TasI AATT 6 cut(s) 113, 323, 542, 552, 626, 754
TfiI GAWTC 1 cut(s) 515
Tru1I TTAA 4 cut(s) 252, 465, 609, 693
Tru9I TTAA 4 cut(s) 252, 465, 609, 693
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 3 cut(s) 52, 484, 709
TseI GCWGC 1 cut(s) 77
Tsp45I GTSAC 3 cut(s) 52, 484, 709
TspDTI ATGAA 4 cut(s) 120, 236, 377, 431
TspGWI ACGGA 1 cut(s) 483
TspRI CASTG 1 cut(s) 64
VpaK11BI GGWCC 1 cut(s) 684
XapI RAATTY 3 cut(s) 113, 323, 626
XhoI CTCGAG 1 cut(s) 437
XspI CTAG 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.