RLG00000035216

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006048
Physical Location & Seq
Forward (+)
63043313 .. 63045091
1779 bp
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UTR
Exon/CDS
Intron
RLM00000035216

Sequence Viewer

Length: 834 bp
ATGTTGAAGCTACCCAGCCTATTAGTTTTGGTTTGCAGCATTGTTCTTGTTTTGCATTCATACCAAGCAACCTCACATGATGAACATGGTGAAGTTGAGGATGAGAGGGAGTTTAATTATGACGACAATAGTGAGAAGGGGCCAGCTAGATGGGGAATGATACGCCATGAATGGAGCACATGTAACAATGGATCGTTGCAGTCTCCCATAGATCTGTTAGATGACAGAGTTGATGTAGTCTCAGATTTAGGGAAACTTCAGGGTAACTACAGGGCTTGCAATGCCACCCTTAAGAATAGAGGCCATGATATGATGTTGAAATGGCAAGCTGATGCAGGATATATTCCAATCAATGGAACTCAGTATAGAGTCAGACAATGCCATTGGCACTCACCTTCTGAACACACCATCAATGGCAAGCAGTTTGATTTGGAGGTGCATCTGGTTCATGACAGTCCAAATGGAAGCATTGCTGTTATCGGGATCCTGTATAAGTTTGGAAAACCTGACCCATTTTTGACACGGATGATGGACTACTTAGAAGACTTATCTGATAGCAGTAAAGAAGAGAAAGTGATTGGTATGGTTGATCCAAAGCAAATAAAAACTGTCAGTACAAAGTACTACAGATATATAGGCTCTCTTACAACTCCTCCTTGCACACAAAATGTCCTCTGGACCCTTGTTGGAGAGGTGAGGACTGTCTCTAAAGAACAGGTCGAATTACTCCGTAAAGCTGTTCACGATGAGTCAGAAACTAATGCAAGACCAGTACAACCGATAAACAGGCGCTCAGTGAAACTCTATAAACCCAATACACAGGATCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0008150 GO:0010033 GO:0010037 GO:0016020 GO:0042221 GO:0042493 GO:0050896 GO:0072347 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

278

Amino Acids

31.62

Weight (kDa)

6.12

Isoelectric Point (pI)

32.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 47 - 267 3.7e-43 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 353
AclWI GGATC 5 cut(s) 199, 478, 491, 584, 831
AcuI CTGAAG 1 cut(s) 242
AfaI GTAC 3 cut(s) 616, 623, 774
AfiI CCNNNNNNNGG 1 cut(s) 353
AflII CTTAAG 1 cut(s) 290
AflIII ACRYGT 1 cut(s) 179
AgsI TTSAA 2 cut(s) 7, 319
AluBI AGCT 4 cut(s) 10, 146, 329, 737
AluI AGCT 4 cut(s) 10, 146, 329, 737
Alw21I GWGCWC 1 cut(s) 179
Alw26I GTCTC 3 cut(s) 207, 244, 709
AlwI GGATC 5 cut(s) 199, 478, 491, 584, 831
AoxI GGCC 2 cut(s) 140, 301
ApeKI GCWGC 1 cut(s) 36
AspLEI GCGC 1 cut(s) 792
AspS9I GGNCC 2 cut(s) 140, 678
AsuHPI GGTGA 3 cut(s) 101, 384, 706
AvaII GGWCC 1 cut(s) 678
BamHI GGATCC 1 cut(s) 483
BbsI GAAGAC 1 cut(s) 549
Bbv12I GWGCWC 1 cut(s) 179
BbvI GCAGC 1 cut(s) 48
BccI CCATC 3 cut(s) 144, 416, 523
BcoDI GTCTC 3 cut(s) 207, 244, 709
BfaI CTAG 1 cut(s) 147
BfmI CTRYAG 2 cut(s) 268, 625
BfoI RGCGCY 1 cut(s) 793
BfrI CTTAAG 1 cut(s) 290
BglII AGATCT 1 cut(s) 211
BisI GCNGC 1 cut(s) 37
BlsI GCNGC 1 cut(s) 38
BmcAI AGTACT 1 cut(s) 623
Bme18I GGWCC 1 cut(s) 678
BmgT120I GGNCC 2 cut(s) 140, 678
BmiI GGNNCC 3 cut(s) 141, 485, 680
BmsI GCATC 2 cut(s) 322, 448
BpiI GAAGAC 1 cut(s) 549
BsaXI ACNNNNNCTCC 4 cut(s) 166, 196, 637, 667
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse1I ACTGG 1 cut(s) 770
Bse3DI GCAATG 2 cut(s) 286, 468
BseGI GGATG 2 cut(s) 106, 531
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMI GCAATG 2 cut(s) 286, 468
BseMII CTCAG 3 cut(s) 255, 374, 807
BseNI ACTGG 1 cut(s) 770
BseRI GAGGAG 1 cut(s) 642
BseXI GCAGC 1 cut(s) 48
BseYI CCCAGC 1 cut(s) 14
BshFI GGCC 2 cut(s) 142, 303
BsiHKAI GWGCWC 1 cut(s) 179
BslI CCNNNNNNNGG 1 cut(s) 353
BsmAI GTCTC 3 cut(s) 207, 244, 709
BsmI GAATGC 1 cut(s) 55
BsnI GGCC 2 cut(s) 142, 303
Bsp1286I GDGCHC 1 cut(s) 179
Bsp143I GATC 5 cut(s) 191, 211, 483, 589, 823
BspANI GGCC 2 cut(s) 142, 303
BspCNI CTCAG 3 cut(s) 254, 373, 806
BspHI TCATGA 1 cut(s) 448
BspLI GGNNCC 3 cut(s) 141, 485, 680
BspPI GGATC 5 cut(s) 199, 478, 491, 584, 831
BspTI CTTAAG 1 cut(s) 290
BsrDI GCAATG 2 cut(s) 286, 468
BsrI ACTGG 1 cut(s) 770
BssMI GATC 5 cut(s) 191, 211, 483, 589, 823
Bst4CI ACNGT 3 cut(s) 455, 610, 703
Bst6I CTCTTC 1 cut(s) 561
BstAFI CTTAAG 1 cut(s) 290
BstC8I GCNNGC 4 cut(s) 144, 277, 327, 419
BstDEI CTNAG 4 cut(s) 241, 360, 538, 793
BstF5I GGATG 2 cut(s) 106, 531
BstH2I RGCGCY 1 cut(s) 793
BstHHI GCGC 1 cut(s) 792
BstKTI GATC 5 cut(s) 194, 214, 486, 592, 826
BstMAI GTCTC 3 cut(s) 207, 244, 709
BstMBI GATC 5 cut(s) 191, 211, 483, 589, 823
BstMWI GCNNNNNNNGC 1 cut(s) 281
BstNSI RCATGY 1 cut(s) 183
BstSFI CTRYAG 2 cut(s) 268, 625
BstV1I GCAGC 1 cut(s) 48
BstV2I GAAGAC 1 cut(s) 549
BstX2I RGATCY 2 cut(s) 211, 483
BstXI CCANNNNNNTGG 1 cut(s) 150
BstYI RGATCY 2 cut(s) 211, 483
BsuRI GGCC 2 cut(s) 142, 303
BtsCI GGATG 2 cut(s) 106, 531
BtsIMutI CAGTG 1 cut(s) 801
Cac8I GCNNGC 4 cut(s) 144, 277, 327, 419
CciI TCATGA 1 cut(s) 448
CfoI GCGC 1 cut(s) 792
Cfr13I GGNCC 2 cut(s) 140, 678
Csp6I GTAC 3 cut(s) 615, 622, 773
CviAII CATG 6 cut(s) 77, 86, 167, 180, 305, 449
CviJI RGCY 9 cut(s) 10, 18, 142, 146, 275, 303, 329, 639, 737
CviKI_1 RGCY 9 cut(s) 10, 18, 142, 146, 275, 303, 329, 639, 737
CviQI GTAC 3 cut(s) 615, 622, 773
DdeI CTNAG 4 cut(s) 241, 360, 538, 793
DpnI GATC 5 cut(s) 193, 213, 485, 591, 825
DpnII GATC 5 cut(s) 191, 211, 483, 589, 823
Eam1104I CTCTTC 1 cut(s) 561
EarI CTCTTC 1 cut(s) 561
Eco47I GGWCC 1 cut(s) 678
Eco57I CTGAAG 1 cut(s) 242
FaeI CATG 6 cut(s) 80, 89, 170, 183, 308, 452
FatI CATG 6 cut(s) 76, 85, 166, 179, 304, 448
Fnu4HI GCNGC 1 cut(s) 37
FokI GGATG 2 cut(s) 113, 538
Fsp4HI GCNGC 1 cut(s) 37
FspBI CTAG 1 cut(s) 147
GlaI GCGC 1 cut(s) 791
GluI GCNGC 1 cut(s) 37
GsaI CCCAGC 1 cut(s) 18
HaeII RGCGCY 1 cut(s) 793
HaeIII GGCC 2 cut(s) 142, 303
HhaI GCGC 1 cut(s) 792
Hin1II CATG 6 cut(s) 80, 89, 170, 183, 308, 452
Hin6I GCGC 1 cut(s) 790
HinP1I GCGC 1 cut(s) 790
HinfI GANTC 2 cut(s) 369, 749
HphI GGTGA 3 cut(s) 101, 384, 706
Hpy166II GTNNAC 1 cut(s) 742
Hpy188I TCNGA 6 cut(s) 244, 374, 400, 553, 754, 828
Hpy188III TCNNGA 4 cut(s) 449, 481, 676, 743
Hpy8I GTNNAC 1 cut(s) 742
HpyAV CCTTC 2 cut(s) 130, 405
HpyCH4III ACNGT 3 cut(s) 455, 610, 703
HpyCH4V TGCA 8 cut(s) 36, 55, 199, 279, 335, 439, 660, 764
HpyF10VI GCNNNNNNNGC 1 cut(s) 281
HpyF3I CTNAG 4 cut(s) 241, 360, 538, 793
Hsp92II CATG 6 cut(s) 80, 89, 170, 183, 308, 452
HspAI GCGC 1 cut(s) 790
Kzo9I GATC 5 cut(s) 191, 211, 483, 589, 823
LmnI GCTCC 1 cut(s) 174
Lsp1109I GCAGC 1 cut(s) 48
LweI GCATC 2 cut(s) 322, 448
MaeI CTAG 1 cut(s) 147
MaeIII GTNAC 2 cut(s) 182, 263
MalI GATC 5 cut(s) 193, 213, 485, 591, 825
MboI GATC 5 cut(s) 191, 211, 483, 589, 823
MboII GAAGA 2 cut(s) 554, 578
MflI RGATCY 2 cut(s) 211, 483
MhlI GDGCHC 1 cut(s) 179
MluCI AATT 3 cut(s) 115, 722, 829
MlyI GAGTC 2 cut(s) 378, 758
MmeI TCCRAC 1 cut(s) 667
MnlI CCTC 9 cut(s) 82, 91, 99, 293, 427, 663, 683, 685, 690
MseI TTAA 2 cut(s) 114, 291
MspCI CTTAAG 1 cut(s) 290
Mva1269I GAATGC 1 cut(s) 55
MwoI GCNNNNNNNGC 1 cut(s) 281
NdeII GATC 5 cut(s) 191, 211, 483, 589, 823
NlaIII CATG 6 cut(s) 80, 89, 170, 183, 308, 452
NlaIV GGNNCC 3 cut(s) 141, 485, 680
NspI RCATGY 1 cut(s) 183
PagI TCATGA 1 cut(s) 448
PciI ACATGT 1 cut(s) 179
PctI GAATGC 1 cut(s) 55
PflMI CCANNNNNTGG 1 cut(s) 353
PkrI GCNGC 1 cut(s) 38
PleI GAGTC 2 cut(s) 377, 757
PpsI GAGTC 2 cut(s) 377, 757
PscI ACATGT 1 cut(s) 179
PspFI CCCAGC 1 cut(s) 14
PspN4I GGNNCC 3 cut(s) 141, 485, 680
PspPI GGNCC 2 cut(s) 140, 678
PsuI RGATCY 2 cut(s) 211, 483
RsaI GTAC 3 cut(s) 616, 623, 774
RsaNI GTAC 3 cut(s) 615, 622, 773
SaqAI TTAA 2 cut(s) 114, 291
SatI GCNGC 1 cut(s) 37
Sau3AI GATC 5 cut(s) 191, 211, 483, 589, 823
Sau96I GGNCC 2 cut(s) 140, 678
ScaI AGTACT 1 cut(s) 623
SchI GAGTC 2 cut(s) 378, 758
SduI GDGCHC 1 cut(s) 179
SfaNI GCATC 2 cut(s) 322, 448
SfcI CTRYAG 2 cut(s) 268, 625
SinI GGWCC 1 cut(s) 678
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 3 cut(s) 115, 722, 829
SspMI CTAG 1 cut(s) 147
TaaI ACNGT 3 cut(s) 455, 610, 703
TaqI TCGA 1 cut(s) 720
TasI AATT 3 cut(s) 115, 722, 829
TatI WGTACW 3 cut(s) 614, 621, 772
Tru1I TTAA 2 cut(s) 114, 291
Tru9I TTAA 2 cut(s) 114, 291
TscAI CASTG 1 cut(s) 801
TseI GCWGC 1 cut(s) 36
TspDTI ATGAA 4 cut(s) 48, 96, 183, 437
TspGWI ACGGA 2 cut(s) 538, 719
TspRI CASTG 1 cut(s) 801
Van91I CCANNNNNTGG 1 cut(s) 353
Vha464I CTTAAG 1 cut(s) 290
VpaK11BI GGWCC 1 cut(s) 678
XceI RCATGY 1 cut(s) 183
XspI CTAG 1 cut(s) 147
ZrmI AGTACT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.