Rh5AG368100

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0006048
Physical Location & Seq
Reverse (-)
62152184 .. 62153969
1786 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG368100.1

Sequence Viewer

Length: 834 bp
ATGTTGAAGCTACCCAGCCTATTAGTTTTGGTTTGCAGCATTGTTCTTGTTTTGCATTCATACCAAGCAACCTCACATGATGAACATGATGAAGTTGAGGATCAGATGGAGTTTAGTTATGACGATGACAGTGAGAAGGGGCCAGCTAGATGGGGAAAGATACGCCACGAATGGAGCATGTGTAACAATGGATCGTTGCAGTCTCCGATCGATCTGTTAGATGACAGAGTTGATGTAGTCTCAGATTTAGGGACACTTCAGGATAGCTACAGGGCTTGCAATGCCACTCTTAAGAATAGAGGCCATGATATGATGTTGAAATGGCAAGCTGATGCAGGATACATTCCAATCAATGGAACTCAGTATACAGTCAGACAATGCCACTGGCACTCACCTTCTGAACACACCATCAATGGCAAGCAGTTTGATTTAGAGTTGCATCTGGTTCATGACAGTCCAAATGCAAGCATCGCTGTTATCGGAATCCTGTATAAGATTGGAAAACCAGACCCATTTTTGACACAGATGATGGACCACTTGGAAGACTTATCTGATAGCAGTAACGAAGAGAAAGTGATTGGTATGGTTGATCCAAAGCAAATAAAAATTGTCAGTACGAAGTACTACAGATATATAGGCTCTCTTACAACTCCTCCTTGCACACAAGATGTCCTATGGACCCTTGTTGGAGAGGTGAGGACTGTCTCTAAAGAACAGGTCAAATTCCTCCGCAAAGCTGTTCACGATGAGTCGGAAACTAATGCAAGACCGGTACAACCGATAAACAGGCGCTCGGTGAAACTTTATAAACCCAGTACACAGGATCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0008150 GO:0010033 GO:0010037 GO:0016020 GO:0042221 GO:0042493 GO:0050896 GO:0072347 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

277

Amino Acids

31.55

Weight (kDa)

5.61

Isoelectric Point (pI)

36.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 47 - 267 8.5e-43 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 807
AccB7I CCANNNNNTGG 1 cut(s) 353
AccI GTMKAC 1 cut(s) 365
AciI CCGC 1 cut(s) 730
AclWI GGATC 4 cut(s) 108, 199, 584, 831
AcsI RAATTY 1 cut(s) 722
AcuI CTGAAG 1 cut(s) 242
AfaI GTAC 4 cut(s) 616, 623, 774, 817
AfiI CCNNNNNNNGG 1 cut(s) 353
AflII CTTAAG 1 cut(s) 290
AgeI ACCGGT 1 cut(s) 769
AgsI TTSAA 2 cut(s) 7, 319
AluBI AGCT 5 cut(s) 10, 146, 267, 329, 737
AluI AGCT 5 cut(s) 10, 146, 267, 329, 737
Alw26I GTCTC 3 cut(s) 207, 244, 709
AlwI GGATC 4 cut(s) 108, 199, 584, 831
AoxI GGCC 2 cut(s) 140, 301
ApeKI GCWGC 1 cut(s) 36
ApoI RAATTY 1 cut(s) 722
AsiGI ACCGGT 1 cut(s) 769
AspLEI GCGC 1 cut(s) 792
AspS9I GGNCC 3 cut(s) 140, 532, 678
AsuHPI GGTGA 3 cut(s) 384, 706, 808
AvaII GGWCC 2 cut(s) 532, 678
BbsI GAAGAC 1 cut(s) 549
BbvI GCAGC 1 cut(s) 48
BccI CCATC 4 cut(s) 100, 144, 416, 523
BciVI GTATCC 1 cut(s) 332
BcoDI GTCTC 3 cut(s) 207, 244, 709
BfaI CTAG 1 cut(s) 147
BfmI CTRYAG 2 cut(s) 268, 625
BfoI RGCGCY 1 cut(s) 793
BfrI CTTAAG 1 cut(s) 290
BfuI GTATCC 1 cut(s) 332
BisI GCNGC 1 cut(s) 37
BlsI GCNGC 1 cut(s) 38
BmcAI AGTACT 1 cut(s) 623
Bme18I GGWCC 2 cut(s) 532, 678
BmgT120I GGNCC 3 cut(s) 140, 532, 678
BmiI GGNNCC 2 cut(s) 141, 680
BmrI ACTGGG 1 cut(s) 807
BmsI GCATC 3 cut(s) 322, 448, 477
BmuI ACTGGG 1 cut(s) 807
BpiI GAAGAC 1 cut(s) 549
Bsa29I ATCGAT 1 cut(s) 210
BsaWI WCCGGW 1 cut(s) 769
BsaXI ACNNNNNCTCC 4 cut(s) 166, 196, 637, 667
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse118I RCCGGY 1 cut(s) 769
Bse1I ACTGG 2 cut(s) 389, 813
Bse3DI GCAATG 1 cut(s) 286
BseCI ATCGAT 1 cut(s) 210
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMI GCAATG 1 cut(s) 286
BseMII CTCAG 2 cut(s) 255, 374
BseNI ACTGG 2 cut(s) 389, 813
BseRI GAGGAG 1 cut(s) 642
BseXI GCAGC 1 cut(s) 48
BseYI CCCAGC 1 cut(s) 14
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 2 cut(s) 142, 303
BshTI ACCGGT 1 cut(s) 769
BshVI ATCGAT 1 cut(s) 210
BsiEI CGRYCG 1 cut(s) 210
BsiSI CCGG 1 cut(s) 770
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 1 cut(s) 353
BsmAI GTCTC 3 cut(s) 207, 244, 709
BsmFI GGGAC 1 cut(s) 265
BsmI GAATGC 1 cut(s) 55
BsnI GGCC 2 cut(s) 142, 303
Bsp143I GATC 6 cut(s) 100, 191, 207, 211, 589, 823
BspACI CCGC 1 cut(s) 730
BspANI GGCC 2 cut(s) 142, 303
BspCNI CTCAG 2 cut(s) 254, 373
BspDI ATCGAT 1 cut(s) 210
BspHI TCATGA 1 cut(s) 448
BspLI GGNNCC 2 cut(s) 141, 680
BspPI GGATC 4 cut(s) 108, 199, 584, 831
BspTI CTTAAG 1 cut(s) 290
BsrDI GCAATG 1 cut(s) 286
BsrFI RCCGGY 1 cut(s) 769
BsrI ACTGG 2 cut(s) 389, 813
BssAI RCCGGY 1 cut(s) 769
BssMI GATC 6 cut(s) 100, 191, 207, 211, 589, 823
BssNAI GTATAC 1 cut(s) 366
Bst1107I GTATAC 1 cut(s) 366
Bst4CI ACNGT 4 cut(s) 131, 370, 455, 703
Bst6I CTCTTC 1 cut(s) 561
BstAFI CTTAAG 1 cut(s) 290
BstC8I GCNNGC 5 cut(s) 144, 277, 327, 419, 466
BstDEI CTNAG 2 cut(s) 241, 360
BstH2I RGCGCY 1 cut(s) 793
BstHHI GCGC 1 cut(s) 792
BstKTI GATC 6 cut(s) 103, 194, 210, 214, 592, 826
BstMAI GTCTC 3 cut(s) 207, 244, 709
BstMBI GATC 6 cut(s) 100, 191, 207, 211, 589, 823
BstMCI CGRYCG 1 cut(s) 210
BstMWI GCNNNNNNNGC 2 cut(s) 281, 470
BstNSI RCATGY 1 cut(s) 181
BstSFI CTRYAG 2 cut(s) 268, 625
BstV1I GCAGC 1 cut(s) 48
BstV2I GAAGAC 1 cut(s) 549
BstXI CCANNNNNNTGG 1 cut(s) 150
BstZ17I GTATAC 1 cut(s) 366
Bsu15I ATCGAT 1 cut(s) 210
BsuI GTATCC 1 cut(s) 332
BsuRI GGCC 2 cut(s) 142, 303
BsuTUI ATCGAT 1 cut(s) 210
BtgZI GCGATG 1 cut(s) 454
BtsIMutI CAGTG 2 cut(s) 136, 382
Cac8I GCNNGC 5 cut(s) 144, 277, 327, 419, 466
CciI TCATGA 1 cut(s) 448
CfoI GCGC 1 cut(s) 792
Cfr10I RCCGGY 1 cut(s) 769
Cfr13I GGNCC 3 cut(s) 140, 532, 678
ClaI ATCGAT 1 cut(s) 210
Csp6I GTAC 4 cut(s) 615, 622, 773, 816
CspAI ACCGGT 1 cut(s) 769
CviAII CATG 5 cut(s) 77, 86, 178, 305, 449
CviQI GTAC 4 cut(s) 615, 622, 773, 816
DdeI CTNAG 2 cut(s) 241, 360
DpnI GATC 6 cut(s) 102, 193, 209, 213, 591, 825
DpnII GATC 6 cut(s) 100, 191, 207, 211, 589, 823
Eam1104I CTCTTC 1 cut(s) 561
EarI CTCTTC 1 cut(s) 561
Eco47I GGWCC 2 cut(s) 532, 678
Eco57I CTGAAG 1 cut(s) 242
FaeI CATG 5 cut(s) 80, 89, 181, 308, 452
FaqI GGGAC 1 cut(s) 265
FatI CATG 5 cut(s) 76, 85, 177, 304, 448
FblI GTMKAC 1 cut(s) 365
Fnu4HI GCNGC 1 cut(s) 37
Fsp4HI GCNGC 1 cut(s) 37
FspBI CTAG 1 cut(s) 147
GlaI GCGC 1 cut(s) 791
GluI GCNGC 1 cut(s) 37
GsaI CCCAGC 1 cut(s) 18
HaeII RGCGCY 1 cut(s) 793
HaeIII GGCC 2 cut(s) 142, 303
HapII CCGG 1 cut(s) 770
HhaI GCGC 1 cut(s) 792
Hin1II CATG 5 cut(s) 80, 89, 181, 308, 452
Hin6I GCGC 1 cut(s) 790
HinP1I GCGC 1 cut(s) 790
HinfI GANTC 2 cut(s) 483, 749
HpaII CCGG 1 cut(s) 770
HphI GGTGA 3 cut(s) 384, 706, 808
Hpy166II GTNNAC 3 cut(s) 366, 742, 818
Hpy188I TCNGA 9 cut(s) 105, 207, 244, 374, 400, 482, 553, 754, 828
Hpy188III TCNNGA 3 cut(s) 260, 449, 743
Hpy8I GTNNAC 3 cut(s) 366, 742, 818
HpyAV CCTTC 2 cut(s) 130, 405
HpyCH4III ACNGT 4 cut(s) 131, 370, 455, 703
HpyCH4V TGCA 9 cut(s) 36, 55, 199, 279, 335, 439, 464, 660, 764
HpyF10VI GCNNNNNNNGC 2 cut(s) 281, 470
HpyF3I CTNAG 2 cut(s) 241, 360
Hsp92II CATG 5 cut(s) 80, 89, 181, 308, 452
HspAI GCGC 1 cut(s) 790
Kzo9I GATC 6 cut(s) 100, 191, 207, 211, 589, 823
LmnI GCTCC 1 cut(s) 174
Lsp1109I GCAGC 1 cut(s) 48
LweI GCATC 3 cut(s) 322, 448, 477
MaeI CTAG 1 cut(s) 147
MaeIII GTNAC 2 cut(s) 182, 560
MalI GATC 6 cut(s) 102, 193, 209, 213, 591, 825
MboI GATC 6 cut(s) 100, 191, 207, 211, 589, 823
MboII GAAGA 2 cut(s) 554, 578
MluCI AATT 3 cut(s) 606, 722, 829
MlyI GAGTC 1 cut(s) 758
MmeI TCCRAC 2 cut(s) 667, 732
MnlI CCTC 7 cut(s) 82, 91, 293, 663, 685, 690, 737
MseI TTAA 1 cut(s) 291
MspCI CTTAAG 1 cut(s) 290
MspI CCGG 1 cut(s) 770
Mva1269I GAATGC 1 cut(s) 55
MwoI GCNNNNNNNGC 2 cut(s) 281, 470
NdeII GATC 6 cut(s) 100, 191, 207, 211, 589, 823
NlaIII CATG 5 cut(s) 80, 89, 181, 308, 452
NlaIV GGNNCC 2 cut(s) 141, 680
NspI RCATGY 1 cut(s) 181
PagI TCATGA 1 cut(s) 448
PctI GAATGC 1 cut(s) 55
PfeI GAWTC 1 cut(s) 483
PflMI CCANNNNNTGG 1 cut(s) 353
PinAI ACCGGT 1 cut(s) 769
PkrI GCNGC 1 cut(s) 38
Ple19I CGATCG 1 cut(s) 210
PleI GAGTC 1 cut(s) 757
PpsI GAGTC 1 cut(s) 757
PsiI TTATAA 1 cut(s) 807
PspFI CCCAGC 1 cut(s) 14
PspN4I GGNNCC 2 cut(s) 141, 680
PspPI GGNCC 3 cut(s) 140, 532, 678
PsrI GAACNNNNNNTAC 2 cut(s) 349, 381
PvuI CGATCG 1 cut(s) 210
RsaI GTAC 4 cut(s) 616, 623, 774, 817
RsaNI GTAC 4 cut(s) 615, 622, 773, 816
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 1 cut(s) 37
Sau3AI GATC 6 cut(s) 100, 191, 207, 211, 589, 823
Sau96I GGNCC 3 cut(s) 140, 532, 678
ScaI AGTACT 1 cut(s) 623
SchI GAGTC 1 cut(s) 758
SetI ASST 9 cut(s) 12, 74, 148, 269, 331, 397, 696, 720, 739
SfaNI GCATC 3 cut(s) 322, 448, 477
SfcI CTRYAG 2 cut(s) 268, 625
SinI GGWCC 2 cut(s) 532, 678
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 3 cut(s) 606, 722, 829
SsiI CCGC 1 cut(s) 730
SspMI CTAG 1 cut(s) 147
TaaI ACNGT 4 cut(s) 131, 370, 455, 703
TaqI TCGA 1 cut(s) 210
TasI AATT 3 cut(s) 606, 722, 829
TatI WGTACW 2 cut(s) 621, 815
TfiI GAWTC 1 cut(s) 483
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TscAI CASTG 2 cut(s) 136, 389
TseI GCWGC 1 cut(s) 36
TspDTI ATGAA 4 cut(s) 48, 96, 105, 437
TspRI CASTG 2 cut(s) 136, 389
Van91I CCANNNNNTGG 1 cut(s) 353
Vha464I CTTAAG 1 cut(s) 290
VpaK11BI GGWCC 2 cut(s) 532, 678
XapI RAATTY 1 cut(s) 722
XceI RCATGY 1 cut(s) 181
XmiI GTMKAC 1 cut(s) 365
XspI CTAG 1 cut(s) 147
ZrmI AGTACT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.