Rh5AG381000

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0006048
Physical Location & Seq
Forward (+)
65379861 .. 65381663
1803 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG381000.1

Sequence Viewer

Length: 834 bp
ATGTTGAAGCTACCCAGCCTATTAGTTTTGGTTTGCAGCATTGTTCTTGTTTTGCATTCATACCAAGCAACCTCACATGATGAACATGATGAAGTTGAGGATGAGAGGGAGTTTAATTATGACGACAATAGTGAGAAGGGGCCAGCTAGATGGGGAAAGATACGCCATGAATGGAGCATATGTAACAATGGATCGTTGCAGTCTCCCATAGATCTGTTAGATGACAGAGTTGATGTAGTCTCAGATTTAGGGAAACTTCAGGGTAACTACAGGGCTTGCAATGCCACTCTTAAGAATAGAGGCCATGATATGATGTTGAAATGGCATGCTGATGCAGGATATATTCCAATCAATGGAACTCAGTATACAGTCAGACAATGCCATTGGCACTCACCTTCTGAACACACCATCAATGGCAAGCAGTTTGATTTAGAGGTGCATCTGGTTCATGACAGTCCAAATGGAAGCATCGCTGTTATCGGGATCCTGTATAAGTTTGGAAAACCTGACCCATTTTTGACACGGATGATGGACTACTTAGAAGACTTATCTGATAGCAGTAAAGAAGAGAAAGTGATTGGTATGGTTGATCCAAAGCAAATAAAAACTGTCAGTACAAAGTACTACAGATATATAGGCTCTCTTACAACTCCTCCTTGCACACAAAATGTCCTCTGGACCCTTGTTGGAGAGGTGAGGACTGTCTCTAAAGAACAGGTCAAATTCCTCCGTAAAGCTGTTCACGATGAGTCAGAAACTAATGCAAGACCAATACAACAGATAAACAGGCGCTCGGTGAAACTTTATAAACCCAGTACACAGGATCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0008150 GO:0010033 GO:0010037 GO:0016020 GO:0042221 GO:0042493 GO:0050896 GO:0072347 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

277

Amino Acids

31.69

Weight (kDa)

6.31

Isoelectric Point (pI)

33.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 47 - 267 4.9e-42 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 807
AccB7I CCANNNNNTGG 1 cut(s) 353
AccI GTMKAC 1 cut(s) 365
AclWI GGATC 5 cut(s) 199, 478, 491, 584, 831
AcsI RAATTY 1 cut(s) 722
AcuI CTGAAG 1 cut(s) 242
AfaI GTAC 3 cut(s) 616, 623, 817
AfiI CCNNNNNNNGG 1 cut(s) 353
AflII CTTAAG 1 cut(s) 290
AgsI TTSAA 2 cut(s) 7, 319
AluBI AGCT 3 cut(s) 10, 146, 737
AluI AGCT 3 cut(s) 10, 146, 737
Alw26I GTCTC 3 cut(s) 207, 244, 709
AlwI GGATC 5 cut(s) 199, 478, 491, 584, 831
AoxI GGCC 2 cut(s) 140, 301
ApeKI GCWGC 1 cut(s) 36
ApoI RAATTY 1 cut(s) 722
AspLEI GCGC 1 cut(s) 792
AspS9I GGNCC 2 cut(s) 140, 678
AsuHPI GGTGA 3 cut(s) 384, 706, 808
AvaII GGWCC 1 cut(s) 678
BamHI GGATCC 1 cut(s) 483
BbsI GAAGAC 1 cut(s) 549
BbvI GCAGC 1 cut(s) 48
BccI CCATC 3 cut(s) 144, 416, 523
BcoDI GTCTC 3 cut(s) 207, 244, 709
BfaI CTAG 1 cut(s) 147
BfmI CTRYAG 2 cut(s) 268, 625
BfoI RGCGCY 1 cut(s) 793
BfrI CTTAAG 1 cut(s) 290
BglII AGATCT 1 cut(s) 211
BisI GCNGC 1 cut(s) 37
BlsI GCNGC 1 cut(s) 38
BmcAI AGTACT 1 cut(s) 623
Bme18I GGWCC 1 cut(s) 678
BmgT120I GGNCC 2 cut(s) 140, 678
BmiI GGNNCC 3 cut(s) 141, 485, 680
BmrI ACTGGG 1 cut(s) 807
BmsI GCATC 3 cut(s) 322, 448, 477
BmuI ACTGGG 1 cut(s) 807
BpiI GAAGAC 1 cut(s) 549
BsaXI ACNNNNNCTCC 4 cut(s) 166, 196, 637, 667
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse1I ACTGG 1 cut(s) 813
Bse3DI GCAATG 1 cut(s) 286
BseGI GGATG 2 cut(s) 106, 531
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMI GCAATG 1 cut(s) 286
BseMII CTCAG 2 cut(s) 255, 374
BseNI ACTGG 1 cut(s) 813
BseRI GAGGAG 1 cut(s) 642
BseXI GCAGC 1 cut(s) 48
BseYI CCCAGC 1 cut(s) 14
BshFI GGCC 2 cut(s) 142, 303
BslI CCNNNNNNNGG 1 cut(s) 353
BsmAI GTCTC 3 cut(s) 207, 244, 709
BsmI GAATGC 1 cut(s) 55
BsnI GGCC 2 cut(s) 142, 303
Bsp143I GATC 5 cut(s) 191, 211, 483, 589, 823
BspANI GGCC 2 cut(s) 142, 303
BspCNI CTCAG 2 cut(s) 254, 373
BspHI TCATGA 1 cut(s) 448
BspLI GGNNCC 3 cut(s) 141, 485, 680
BspPI GGATC 5 cut(s) 199, 478, 491, 584, 831
BspTI CTTAAG 1 cut(s) 290
BsrDI GCAATG 1 cut(s) 286
BsrI ACTGG 1 cut(s) 813
BssMI GATC 5 cut(s) 191, 211, 483, 589, 823
BssNAI GTATAC 1 cut(s) 366
Bst1107I GTATAC 1 cut(s) 366
Bst4CI ACNGT 4 cut(s) 370, 455, 610, 703
Bst6I CTCTTC 1 cut(s) 561
BstAFI CTTAAG 1 cut(s) 290
BstC8I GCNNGC 4 cut(s) 144, 277, 327, 419
BstDEI CTNAG 3 cut(s) 241, 360, 538
BstF5I GGATG 2 cut(s) 106, 531
BstH2I RGCGCY 1 cut(s) 793
BstHHI GCGC 1 cut(s) 792
BstKTI GATC 5 cut(s) 194, 214, 486, 592, 826
BstMAI GTCTC 3 cut(s) 207, 244, 709
BstMBI GATC 5 cut(s) 191, 211, 483, 589, 823
BstMWI GCNNNNNNNGC 1 cut(s) 281
BstNSI RCATGY 1 cut(s) 329
BstSFI CTRYAG 2 cut(s) 268, 625
BstV1I GCAGC 1 cut(s) 48
BstV2I GAAGAC 1 cut(s) 549
BstX2I RGATCY 2 cut(s) 211, 483
BstXI CCANNNNNNTGG 1 cut(s) 150
BstYI RGATCY 2 cut(s) 211, 483
BstZ17I GTATAC 1 cut(s) 366
BsuRI GGCC 2 cut(s) 142, 303
BtgZI GCGATG 1 cut(s) 454
BtsCI GGATG 2 cut(s) 106, 531
Cac8I GCNNGC 4 cut(s) 144, 277, 327, 419
CciI TCATGA 1 cut(s) 448
CfoI GCGC 1 cut(s) 792
Cfr13I GGNCC 2 cut(s) 140, 678
Csp6I GTAC 3 cut(s) 615, 622, 816
CviAII CATG 6 cut(s) 77, 86, 167, 305, 326, 449
CviJI RGCY 8 cut(s) 10, 18, 142, 146, 275, 303, 639, 737
CviKI_1 RGCY 8 cut(s) 10, 18, 142, 146, 275, 303, 639, 737
CviQI GTAC 3 cut(s) 615, 622, 816
DdeI CTNAG 3 cut(s) 241, 360, 538
DpnI GATC 5 cut(s) 193, 213, 485, 591, 825
DpnII GATC 5 cut(s) 191, 211, 483, 589, 823
Eam1104I CTCTTC 1 cut(s) 561
EarI CTCTTC 1 cut(s) 561
Eco47I GGWCC 1 cut(s) 678
Eco57I CTGAAG 1 cut(s) 242
FaeI CATG 6 cut(s) 80, 89, 170, 308, 329, 452
FatI CATG 6 cut(s) 76, 85, 166, 304, 325, 448
FauNDI CATATG 1 cut(s) 179
FblI GTMKAC 1 cut(s) 365
Fnu4HI GCNGC 1 cut(s) 37
FokI GGATG 2 cut(s) 113, 538
Fsp4HI GCNGC 1 cut(s) 37
FspBI CTAG 1 cut(s) 147
GlaI GCGC 1 cut(s) 791
GluI GCNGC 1 cut(s) 37
GsaI CCCAGC 1 cut(s) 18
HaeII RGCGCY 1 cut(s) 793
HaeIII GGCC 2 cut(s) 142, 303
HhaI GCGC 1 cut(s) 792
Hin1II CATG 6 cut(s) 80, 89, 170, 308, 329, 452
Hin6I GCGC 1 cut(s) 790
HinP1I GCGC 1 cut(s) 790
HinfI GANTC 1 cut(s) 749
HphI GGTGA 3 cut(s) 384, 706, 808
Hpy166II GTNNAC 3 cut(s) 366, 742, 818
Hpy188I TCNGA 6 cut(s) 244, 374, 400, 553, 754, 828
Hpy188III TCNNGA 4 cut(s) 449, 481, 676, 743
Hpy8I GTNNAC 3 cut(s) 366, 742, 818
HpyAV CCTTC 2 cut(s) 130, 405
HpyCH4III ACNGT 4 cut(s) 370, 455, 610, 703
HpyCH4V TGCA 8 cut(s) 36, 55, 199, 279, 335, 439, 660, 764
HpyF10VI GCNNNNNNNGC 1 cut(s) 281
HpyF3I CTNAG 3 cut(s) 241, 360, 538
Hsp92II CATG 6 cut(s) 80, 89, 170, 308, 329, 452
HspAI GCGC 1 cut(s) 790
Kzo9I GATC 5 cut(s) 191, 211, 483, 589, 823
LmnI GCTCC 1 cut(s) 174
Lsp1109I GCAGC 1 cut(s) 48
LweI GCATC 3 cut(s) 322, 448, 477
MaeI CTAG 1 cut(s) 147
MaeIII GTNAC 2 cut(s) 182, 263
MalI GATC 5 cut(s) 193, 213, 485, 591, 825
MboI GATC 5 cut(s) 191, 211, 483, 589, 823
MboII GAAGA 2 cut(s) 554, 578
MflI RGATCY 2 cut(s) 211, 483
MluCI AATT 3 cut(s) 115, 722, 829
MlyI GAGTC 1 cut(s) 758
MmeI TCCRAC 1 cut(s) 667
MseI TTAA 2 cut(s) 114, 291
MslI CAYNNNNRTG 1 cut(s) 330
MspCI CTTAAG 1 cut(s) 290
Mva1269I GAATGC 1 cut(s) 55
MwoI GCNNNNNNNGC 1 cut(s) 281
NdeI CATATG 1 cut(s) 179
NdeII GATC 5 cut(s) 191, 211, 483, 589, 823
NlaIII CATG 6 cut(s) 80, 89, 170, 308, 329, 452
NlaIV GGNNCC 3 cut(s) 141, 485, 680
NspI RCATGY 1 cut(s) 329
PaeI GCATGC 1 cut(s) 329
PagI TCATGA 1 cut(s) 448
PctI GAATGC 1 cut(s) 55
PflMI CCANNNNNTGG 1 cut(s) 353
PkrI GCNGC 1 cut(s) 38
PleI GAGTC 1 cut(s) 757
PpsI GAGTC 1 cut(s) 757
PsiI TTATAA 1 cut(s) 807
PspFI CCCAGC 1 cut(s) 14
PspN4I GGNNCC 3 cut(s) 141, 485, 680
PspPI GGNCC 2 cut(s) 140, 678
PsrI GAACNNNNNNTAC 2 cut(s) 349, 381
PsuI RGATCY 2 cut(s) 211, 483
RsaI GTAC 3 cut(s) 616, 623, 817
RsaNI GTAC 3 cut(s) 615, 622, 816
RseI CAYNNNNRTG 1 cut(s) 330
SaqAI TTAA 2 cut(s) 114, 291
SatI GCNGC 1 cut(s) 37
Sau3AI GATC 5 cut(s) 191, 211, 483, 589, 823
Sau96I GGNCC 2 cut(s) 140, 678
ScaI AGTACT 1 cut(s) 623
SchI GAGTC 1 cut(s) 758
SetI ASST 9 cut(s) 12, 74, 148, 397, 438, 508, 696, 720, 739
SfaNI GCATC 3 cut(s) 322, 448, 477
SfcI CTRYAG 2 cut(s) 268, 625
SinI GGWCC 1 cut(s) 678
SmiMI CAYNNNNRTG 1 cut(s) 330
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SphI GCATGC 1 cut(s) 329
Sse9I AATT 3 cut(s) 115, 722, 829
SspMI CTAG 1 cut(s) 147
TaaI ACNGT 4 cut(s) 370, 455, 610, 703
TasI AATT 3 cut(s) 115, 722, 829
TatI WGTACW 3 cut(s) 614, 621, 815
Tru1I TTAA 2 cut(s) 114, 291
Tru9I TTAA 2 cut(s) 114, 291
TseI GCWGC 1 cut(s) 36
TspDTI ATGAA 5 cut(s) 48, 96, 105, 183, 437
TspGWI ACGGA 2 cut(s) 538, 719
Van91I CCANNNNNTGG 1 cut(s) 353
Vha464I CTTAAG 1 cut(s) 290
VpaK11BI GGWCC 1 cut(s) 678
XapI RAATTY 1 cut(s) 722
XceI RCATGY 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 365
XspI CTAG 1 cut(s) 147
ZrmI AGTACT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.