RLG00000035043

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006048
Physical Location & Seq
Reverse (-)
59750952 .. 59752730
1779 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035043

Sequence Viewer

Length: 834 bp
ATGTTGAAGCTACCCAGCCTATTAGTTTTGGTTTGCTGCATTGTTCTTGTTTTGCATTCATACCAAGCAACCTCAGATGATGAACATGATGAAGTTGAGGATCAGATGGAGTTTAGTTATGACGACGACAGTGAGAAGGGCCCAGCTAGATGGGGAAAGATACGCAATGAATGGAGCATGTGTAACAATGGATCGTTGCAGTCTCCGATCGATCTGTTAGATGACAGAGTTGATGTGGTCTCAGATTTTGGGACACTTCAGGATAGCTACAGGGCTTGCAATGCCACTCTTAAGAATAGAGGCCATGATATGATGTTGAAATGGCAAGCTGATGCAGGATACATTCCAATCAATGGAACTCAGTATACAGCCAGACAATGCCACTGGCACTCACCTTCTGAACACACCATCAATGGCAAGCAGTTTGATTTAGAGTTGCATCTGGTTCATGACAGTCCAAATGCAAGCATCGCTGTTATCGGAATCCTGTATAAGATTGGAAAACCTGACCCATTTTTGACACAGATGATGGACCACTTGGAAGACTTATCTGATAGCAGTAAAGAAGAGAAAGTGATTGGTATGGTTGATCCAAAGCAAATAAAAATTGTCAGTACAAAGTACTACAGATATATAGGCTCTCTTACAACTCCTCCTTGCACACAAGATGTCCTATGGACCCTTGTTGAAGAGGTGAGGACTGTCTCTAAAGAACAGGTCAAATTCCTCCGCGAAGCTGTTCACGATGAGTCAGAAACTAATGCAAGACCAGTACAACCGATAAACAGGCGCTCGGTGAAACTTTATAAACCCAGTACACAGGATCCGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0008150 GO:0010033 GO:0010037 GO:0016020 GO:0042221 GO:0042493 GO:0050896 GO:0072347 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

278

Amino Acids

31.58

Weight (kDa)

5.18

Isoelectric Point (pI)

37.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 47 - 267 2.6e-43 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 807
AccB7I CCANNNNNTGG 1 cut(s) 353
AccI GTMKAC 1 cut(s) 365
AccII CGCG 1 cut(s) 732
AciI CCGC 1 cut(s) 730
AclWI GGATC 5 cut(s) 108, 199, 584, 818, 831
AcsI RAATTY 1 cut(s) 722
AcuI CTGAAG 1 cut(s) 242
AfaI GTAC 4 cut(s) 616, 623, 774, 817
AfiI CCNNNNNNNGG 1 cut(s) 353
AflII CTTAAG 1 cut(s) 290
AgsI TTSAA 3 cut(s) 7, 319, 689
AluBI AGCT 5 cut(s) 10, 146, 267, 329, 737
AluI AGCT 5 cut(s) 10, 146, 267, 329, 737
Alw26I GTCTC 3 cut(s) 207, 244, 709
AlwI GGATC 5 cut(s) 108, 199, 584, 818, 831
AoxI GGCC 2 cut(s) 139, 301
ApaI GGGCCC 1 cut(s) 143
ApeKI GCWGC 1 cut(s) 36
ApoI RAATTY 1 cut(s) 722
Asp700I GAANNNNTTC 1 cut(s) 738
AspLEI GCGC 1 cut(s) 792
AspS9I GGNCC 4 cut(s) 139, 140, 532, 678
AsuHPI GGTGA 3 cut(s) 384, 706, 808
AvaII GGWCC 2 cut(s) 532, 678
BaeGI GKGCMC 1 cut(s) 143
BamHI GGATCC 1 cut(s) 823
BanII GRGCYC 1 cut(s) 143
BbsI GAAGAC 1 cut(s) 549
BbvI GCAGC 1 cut(s) 23
BccI CCATC 4 cut(s) 100, 144, 416, 523
BciVI GTATCC 1 cut(s) 332
BcoDI GTCTC 3 cut(s) 207, 244, 709
BfaI CTAG 1 cut(s) 147
BfmI CTRYAG 2 cut(s) 268, 625
BfoI RGCGCY 1 cut(s) 793
BfrI CTTAAG 1 cut(s) 290
BfuI GTATCC 1 cut(s) 332
BisI GCNGC 1 cut(s) 37
BlsI GCNGC 1 cut(s) 38
BmcAI AGTACT 1 cut(s) 623
Bme18I GGWCC 2 cut(s) 532, 678
BmgT120I GGNCC 4 cut(s) 139, 140, 532, 678
BmiI GGNNCC 3 cut(s) 141, 680, 825
BmrI ACTGGG 1 cut(s) 807
BmsI GCATC 3 cut(s) 322, 448, 477
BmuI ACTGGG 1 cut(s) 807
BpiI GAAGAC 1 cut(s) 549
Bsa29I ATCGAT 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 244
BsaXI ACNNNNNCTCC 4 cut(s) 166, 196, 637, 667
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse1I ACTGG 3 cut(s) 389, 770, 813
Bse3DI GCAATG 2 cut(s) 172, 286
BseCI ATCGAT 1 cut(s) 210
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMI GCAATG 2 cut(s) 172, 286
BseMII CTCAG 3 cut(s) 87, 255, 374
BseNI ACTGG 3 cut(s) 389, 770, 813
BseRI GAGGAG 1 cut(s) 642
BseSI GKGCMC 1 cut(s) 143
BseXI GCAGC 1 cut(s) 23
BseYI CCCAGC 2 cut(s) 14, 142
Bsh1236I CGCG 1 cut(s) 732
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 2 cut(s) 141, 303
BshVI ATCGAT 1 cut(s) 210
BsiEI CGRYCG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 1 cut(s) 353
BsmAI GTCTC 3 cut(s) 207, 244, 709
BsmFI GGGAC 1 cut(s) 265
BsmI GAATGC 1 cut(s) 55
BsnI GGCC 2 cut(s) 141, 303
Bso31I GGTCTC 1 cut(s) 244
Bsp120I GGGCCC 1 cut(s) 139
Bsp1286I GDGCHC 1 cut(s) 143
Bsp143I GATC 6 cut(s) 100, 191, 207, 211, 589, 823
BspACI CCGC 1 cut(s) 730
BspANI GGCC 2 cut(s) 141, 303
BspCNI CTCAG 3 cut(s) 86, 254, 373
BspDI ATCGAT 1 cut(s) 210
BspFNI CGCG 1 cut(s) 732
BspHI TCATGA 1 cut(s) 448
BspLI GGNNCC 3 cut(s) 141, 680, 825
BspPI GGATC 5 cut(s) 108, 199, 584, 818, 831
BspTI CTTAAG 1 cut(s) 290
BspTNI GGTCTC 1 cut(s) 244
BsrDI GCAATG 2 cut(s) 172, 286
BsrI ACTGG 3 cut(s) 389, 770, 813
BssMI GATC 6 cut(s) 100, 191, 207, 211, 589, 823
BssNAI GTATAC 1 cut(s) 366
Bst1107I GTATAC 1 cut(s) 366
Bst4CI ACNGT 3 cut(s) 131, 455, 703
Bst6I CTCTTC 2 cut(s) 561, 684
BstAFI CTTAAG 1 cut(s) 290
BstC8I GCNNGC 4 cut(s) 277, 327, 419, 466
BstDEI CTNAG 3 cut(s) 73, 241, 360
BstFNI CGCG 1 cut(s) 732
BstH2I RGCGCY 1 cut(s) 793
BstHHI GCGC 1 cut(s) 792
BstKTI GATC 6 cut(s) 103, 194, 210, 214, 592, 826
BstMAI GTCTC 3 cut(s) 207, 244, 709
BstMBI GATC 6 cut(s) 100, 191, 207, 211, 589, 823
BstMCI CGRYCG 1 cut(s) 210
BstMWI GCNNNNNNNGC 2 cut(s) 281, 470
BstNSI RCATGY 1 cut(s) 181
BstSFI CTRYAG 2 cut(s) 268, 625
BstSLI GKGCMC 1 cut(s) 143
BstUI CGCG 1 cut(s) 732
BstV1I GCAGC 1 cut(s) 23
BstV2I GAAGAC 1 cut(s) 549
BstX2I RGATCY 1 cut(s) 823
BstXI CCANNNNNNTGG 1 cut(s) 150
BstYI RGATCY 1 cut(s) 823
BstZ17I GTATAC 1 cut(s) 366
Bsu15I ATCGAT 1 cut(s) 210
BsuI GTATCC 1 cut(s) 332
BsuRI GGCC 2 cut(s) 141, 303
BsuTUI ATCGAT 1 cut(s) 210
BtgZI GCGATG 1 cut(s) 454
BtsIMutI CAGTG 2 cut(s) 136, 382
Cac8I GCNNGC 4 cut(s) 277, 327, 419, 466
CciI TCATGA 1 cut(s) 448
CfoI GCGC 1 cut(s) 792
Cfr13I GGNCC 4 cut(s) 139, 140, 532, 678
ClaI ATCGAT 1 cut(s) 210
Csp6I GTAC 4 cut(s) 615, 622, 773, 816
CviAII CATG 4 cut(s) 86, 178, 305, 449
CviQI GTAC 4 cut(s) 615, 622, 773, 816
DdeI CTNAG 3 cut(s) 73, 241, 360
DpnI GATC 6 cut(s) 102, 193, 209, 213, 591, 825
DpnII GATC 6 cut(s) 100, 191, 207, 211, 589, 823
Eam1104I CTCTTC 2 cut(s) 561, 684
EarI CTCTTC 2 cut(s) 561, 684
Eco24I GRGCYC 1 cut(s) 143
Eco31I GGTCTC 1 cut(s) 244
Eco47I GGWCC 2 cut(s) 532, 678
Eco57I CTGAAG 1 cut(s) 242
EcoO109I RGGNCCY 1 cut(s) 139
EcoT38I GRGCYC 1 cut(s) 143
FaeI CATG 4 cut(s) 89, 181, 308, 452
FaqI GGGAC 1 cut(s) 265
FatI CATG 4 cut(s) 85, 177, 304, 448
FblI GTMKAC 1 cut(s) 365
Fnu4HI GCNGC 1 cut(s) 37
FriOI GRGCYC 1 cut(s) 143
Fsp4HI GCNGC 1 cut(s) 37
FspBI CTAG 1 cut(s) 147
GlaI GCGC 1 cut(s) 791
GluI GCNGC 1 cut(s) 37
GsaI CCCAGC 2 cut(s) 18, 146
HaeII RGCGCY 1 cut(s) 793
HaeIII GGCC 2 cut(s) 141, 303
HhaI GCGC 1 cut(s) 792
Hin1II CATG 4 cut(s) 89, 181, 308, 452
Hin6I GCGC 1 cut(s) 790
HinP1I GCGC 1 cut(s) 790
HinfI GANTC 2 cut(s) 483, 749
HphI GGTGA 3 cut(s) 384, 706, 808
Hpy166II GTNNAC 3 cut(s) 366, 742, 818
Hpy188I TCNGA 9 cut(s) 76, 105, 207, 244, 400, 482, 553, 754, 828
Hpy188III TCNNGA 3 cut(s) 260, 449, 743
Hpy8I GTNNAC 3 cut(s) 366, 742, 818
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 2 cut(s) 130, 405
HpyCH4III ACNGT 3 cut(s) 131, 455, 703
HpyCH4V TGCA 9 cut(s) 39, 55, 199, 279, 335, 439, 464, 660, 764
HpyF10VI GCNNNNNNNGC 2 cut(s) 281, 470
HpyF3I CTNAG 3 cut(s) 73, 241, 360
Hsp92II CATG 4 cut(s) 89, 181, 308, 452
HspAI GCGC 1 cut(s) 790
Kzo9I GATC 6 cut(s) 100, 191, 207, 211, 589, 823
LmnI GCTCC 1 cut(s) 174
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 3 cut(s) 322, 448, 477
MaeI CTAG 1 cut(s) 147
MaeIII GTNAC 1 cut(s) 182
MalI GATC 6 cut(s) 102, 193, 209, 213, 591, 825
MboI GATC 6 cut(s) 100, 191, 207, 211, 589, 823
MboII GAAGA 3 cut(s) 554, 578, 701
MflI RGATCY 1 cut(s) 823
MhlI GDGCHC 1 cut(s) 143
MluCI AATT 3 cut(s) 606, 722, 829
MlyI GAGTC 1 cut(s) 758
MnlI CCTC 7 cut(s) 82, 91, 293, 663, 685, 690, 737
MroXI GAANNNNTTC 1 cut(s) 738
MseI TTAA 1 cut(s) 291
MspCI CTTAAG 1 cut(s) 290
Mva1269I GAATGC 1 cut(s) 55
MvnI CGCG 1 cut(s) 732
MwoI GCNNNNNNNGC 2 cut(s) 281, 470
NdeII GATC 6 cut(s) 100, 191, 207, 211, 589, 823
NlaIII CATG 4 cut(s) 89, 181, 308, 452
NlaIV GGNNCC 3 cut(s) 141, 680, 825
NspI RCATGY 1 cut(s) 181
PagI TCATGA 1 cut(s) 448
PctI GAATGC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 738
PfeI GAWTC 1 cut(s) 483
PflMI CCANNNNNTGG 1 cut(s) 353
PkrI GCNGC 1 cut(s) 38
Ple19I CGATCG 1 cut(s) 210
PleI GAGTC 1 cut(s) 757
PpsI GAGTC 1 cut(s) 757
PsiI TTATAA 1 cut(s) 807
PspFI CCCAGC 2 cut(s) 14, 142
PspN4I GGNNCC 3 cut(s) 141, 680, 825
PspOMI GGGCCC 1 cut(s) 139
PspPI GGNCC 4 cut(s) 139, 140, 532, 678
PsrI GAACNNNNNNTAC 2 cut(s) 349, 381
PsuI RGATCY 1 cut(s) 823
PvuI CGATCG 1 cut(s) 210
RsaI GTAC 4 cut(s) 616, 623, 774, 817
RsaNI GTAC 4 cut(s) 615, 622, 773, 816
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 1 cut(s) 37
Sau3AI GATC 6 cut(s) 100, 191, 207, 211, 589, 823
Sau96I GGNCC 4 cut(s) 139, 140, 532, 678
ScaI AGTACT 1 cut(s) 623
SchI GAGTC 1 cut(s) 758
SduI GDGCHC 1 cut(s) 143
SfaNI GCATC 3 cut(s) 322, 448, 477
SfcI CTRYAG 2 cut(s) 268, 625
SinI GGWCC 2 cut(s) 532, 678
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 3 cut(s) 606, 722, 829
SsiI CCGC 1 cut(s) 730
SspMI CTAG 1 cut(s) 147
TaaI ACNGT 3 cut(s) 131, 455, 703
TaqI TCGA 1 cut(s) 210
TasI AATT 3 cut(s) 606, 722, 829
TatI WGTACW 4 cut(s) 614, 621, 772, 815
TfiI GAWTC 1 cut(s) 483
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TscAI CASTG 2 cut(s) 136, 389
TseI GCWGC 1 cut(s) 36
TspDTI ATGAA 5 cut(s) 48, 96, 105, 183, 437
TspRI CASTG 2 cut(s) 136, 389
Van91I CCANNNNNTGG 1 cut(s) 353
Vha464I CTTAAG 1 cut(s) 290
VpaK11BI GGWCC 2 cut(s) 532, 678
XapI RAATTY 1 cut(s) 722
XceI RCATGY 1 cut(s) 181
XmiI GTMKAC 1 cut(s) 365
XmnI GAANNNNTTC 1 cut(s) 738
XspI CTAG 1 cut(s) 147
ZrmI AGTACT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.