RchiOBHm_Chr5g0056011

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006048
Physical Location & Seq
Reverse (-)
59459581 .. 59460700
1120 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33294

Sequence Viewer

Length: 831 bp
ATGTTTCTTATTGTTGATGGTGCAGAGGATCAGATGGAGTTTAGTTATGACGACGACAGTGAGAAGGGGCCAGCTAGATGGGGAAAGATACGCCACGAATGGAGCATGTGTAACAATGGATCGTTGCAGTCTCCGATCGATCTGTTAGATGCCAGAGTTGATGTAGTCTCAGATTTAGGGACACTTCAGGATAGCTACAGGGCTTGCAATGCCACTCTTAAGAATAGAGGCCATGATATGATGTTGAAATGGCAAGCTGATGCAGGATACATTCCAATCAATGGAACTCAGTATACAGCCAGACAATGCCACTGGCACTCACCTTCTGAACACACCATCAATGGCAAGCAGTTTGATTTAGAGTTGCATCTGGTTCATGACAGTCCAAATGCAAGCATCGCTGTTATCGGAATCCTGTATAAGATTGGAAAACCAGACCCATTTTTGACACAGATGATGGACCACTTGGAAGACTTATCTGATAGCATTAACGAAGAGAAAGTGATTGGTATGGTTGATCCAAAGCAAATAAAAATTGTCAGTACGAAGTACTACAGATATATAGGCTCTCTTACAACTCCTCCTTGCACACAAGATGTCCTATGGACCCTTGTTGGAGAGGTGAGGACTGTCTCTAAAGAACAGGTCAAATTCCTCCGCAAAGCTGTTCACGATGTAAGTTCAATAGCTACTCGATCTCTAGAGTATGATAATCTCTTGACATATATGCTGCATGTTATACTGTCACTGATGAATCAGGATCACGTAATTAACGGTCTTGATTCGGAATCTGGCAACGTAACTAGTCAAACAATTTGGCAAAATATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0008150 GO:0010033 GO:0010037 GO:0016020 GO:0042221 GO:0042493 GO:0050896 GO:0072347 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

276

Amino Acids

31.26

Weight (kDa)

5.2

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 23 - 225 1.7e-37 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 281
AccI GTMKAC 1 cut(s) 293
AciI CCGC 1 cut(s) 658
AclWI GGATC 4 cut(s) 36, 127, 512, 768
AcsI RAATTY 1 cut(s) 650
AcuI CTGAAG 1 cut(s) 170
AfaI GTAC 2 cut(s) 544, 551
AfiI CCNNNNNNNGG 1 cut(s) 281
AflII CTTAAG 1 cut(s) 218
AgsI TTSAA 2 cut(s) 247, 684
AhlI ACTAGT 1 cut(s) 803
AluBI AGCT 5 cut(s) 74, 195, 257, 665, 689
AluI AGCT 5 cut(s) 74, 195, 257, 665, 689
Alw26I GTCTC 3 cut(s) 135, 172, 637
AlwI GGATC 4 cut(s) 36, 127, 512, 768
AoxI GGCC 2 cut(s) 68, 229
ApeKI GCWGC 1 cut(s) 730
ApoI RAATTY 1 cut(s) 650
AspS9I GGNCC 3 cut(s) 68, 460, 606
AsuHPI GGTGA 2 cut(s) 312, 634
AvaII GGWCC 2 cut(s) 460, 606
BbsI GAAGAC 1 cut(s) 477
BbvI GCAGC 1 cut(s) 717
BccI CCATC 5 cut(s) 11, 28, 72, 344, 451
BciVI GTATCC 1 cut(s) 260
BcoDI GTCTC 3 cut(s) 135, 172, 637
BcuI ACTAGT 1 cut(s) 803
BfaI CTAG 3 cut(s) 75, 701, 804
BfmI CTRYAG 2 cut(s) 196, 553
BfrI CTTAAG 1 cut(s) 218
BfuI GTATCC 1 cut(s) 260
BisI GCNGC 1 cut(s) 731
BlsI GCNGC 1 cut(s) 732
BmcAI AGTACT 1 cut(s) 551
Bme18I GGWCC 2 cut(s) 460, 606
BmgT120I GGNCC 3 cut(s) 68, 460, 606
BmiI GGNNCC 2 cut(s) 69, 608
BmsI GCATC 4 cut(s) 139, 250, 376, 405
BpiI GAAGAC 1 cut(s) 477
Bsa29I ATCGAT 1 cut(s) 138
BsaAI YACGTR 1 cut(s) 766
BsaXI ACNNNNNCTCC 4 cut(s) 94, 124, 565, 595
Bsc4I CCNNNNNNNGG 1 cut(s) 281
Bse1I ACTGG 1 cut(s) 317
Bse3DI GCAATG 1 cut(s) 214
BseCI ATCGAT 1 cut(s) 138
BseLI CCNNNNNNNGG 1 cut(s) 281
BseMI GCAATG 1 cut(s) 214
BseMII CTCAG 2 cut(s) 183, 302
BseNI ACTGG 1 cut(s) 317
BseRI GAGGAG 1 cut(s) 570
BseXI GCAGC 1 cut(s) 717
BsgI GTGCAG 1 cut(s) 42
Bsh1285I CGRYCG 1 cut(s) 138
BshFI GGCC 2 cut(s) 70, 231
BshVI ATCGAT 1 cut(s) 138
BsiEI CGRYCG 1 cut(s) 138
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 1 cut(s) 281
BsmAI GTCTC 3 cut(s) 135, 172, 637
BsmFI GGGAC 1 cut(s) 193
BsnI GGCC 2 cut(s) 70, 231
Bsp143I GATC 7 cut(s) 28, 119, 135, 139, 517, 695, 760
BspACI CCGC 1 cut(s) 658
BspANI GGCC 2 cut(s) 70, 231
BspCNI CTCAG 2 cut(s) 182, 301
BspDI ATCGAT 1 cut(s) 138
BspHI TCATGA 1 cut(s) 376
BspLI GGNNCC 2 cut(s) 69, 608
BspPI GGATC 4 cut(s) 36, 127, 512, 768
BspTI CTTAAG 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 214
BsrI ACTGG 1 cut(s) 317
BssMI GATC 7 cut(s) 28, 119, 135, 139, 517, 695, 760
BssNAI GTATAC 1 cut(s) 294
Bst1107I GTATAC 1 cut(s) 294
Bst4CI ACNGT 5 cut(s) 59, 383, 631, 744, 776
Bst6I CTCTTC 1 cut(s) 489
BstAFI CTTAAG 1 cut(s) 218
BstBAI YACGTR 1 cut(s) 766
BstC8I GCNNGC 5 cut(s) 72, 205, 255, 347, 394
BstDEI CTNAG 2 cut(s) 169, 288
BstKTI GATC 7 cut(s) 31, 122, 138, 142, 520, 698, 763
BstMAI GTCTC 3 cut(s) 135, 172, 637
BstMBI GATC 7 cut(s) 28, 119, 135, 139, 517, 695, 760
BstMCI CGRYCG 1 cut(s) 138
BstMWI GCNNNNNNNGC 2 cut(s) 209, 398
BstNSI RCATGY 2 cut(s) 109, 737
BstSFI CTRYAG 2 cut(s) 196, 553
BstV1I GCAGC 1 cut(s) 717
BstV2I GAAGAC 1 cut(s) 477
BstXI CCANNNNNNTGG 1 cut(s) 78
BstZ17I GTATAC 1 cut(s) 294
Bsu15I ATCGAT 1 cut(s) 138
BsuI GTATCC 1 cut(s) 260
BsuRI GGCC 2 cut(s) 70, 231
BsuTUI ATCGAT 1 cut(s) 138
BtgZI GCGATG 1 cut(s) 382
BtsIMutI CAGTG 3 cut(s) 64, 310, 746
Cac8I GCNNGC 5 cut(s) 72, 205, 255, 347, 394
CciI TCATGA 1 cut(s) 376
Cfr13I GGNCC 3 cut(s) 68, 460, 606
ClaI ATCGAT 1 cut(s) 138
Csp6I GTAC 2 cut(s) 543, 550
CviAII CATG 4 cut(s) 106, 233, 377, 734
CviQI GTAC 2 cut(s) 543, 550
DdeI CTNAG 2 cut(s) 169, 288
DpnI GATC 7 cut(s) 30, 121, 137, 141, 519, 697, 762
DpnII GATC 7 cut(s) 28, 119, 135, 139, 517, 695, 760
Eam1104I CTCTTC 1 cut(s) 489
EarI CTCTTC 1 cut(s) 489
Eco47I GGWCC 2 cut(s) 460, 606
Eco57I CTGAAG 1 cut(s) 170
FaeI CATG 4 cut(s) 109, 236, 380, 737
FaqI GGGAC 1 cut(s) 193
FatI CATG 4 cut(s) 105, 232, 376, 733
FblI GTMKAC 1 cut(s) 293
Fnu4HI GCNGC 1 cut(s) 731
Fsp4HI GCNGC 1 cut(s) 731
FspBI CTAG 3 cut(s) 75, 701, 804
GluI GCNGC 1 cut(s) 731
HaeIII GGCC 2 cut(s) 70, 231
Hin1II CATG 4 cut(s) 109, 236, 380, 737
HinfI GANTC 4 cut(s) 411, 754, 782, 788
HphI GGTGA 2 cut(s) 312, 634
Hpy166II GTNNAC 2 cut(s) 294, 670
Hpy188I TCNGA 7 cut(s) 33, 135, 172, 328, 410, 481, 787
Hpy188III TCNNGA 7 cut(s) 188, 377, 671, 701, 718, 758, 779
Hpy8I GTNNAC 2 cut(s) 294, 670
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 2 cut(s) 58, 333
HpyCH4III ACNGT 5 cut(s) 59, 383, 631, 744, 776
HpyCH4IV ACGT 2 cut(s) 765, 798
HpyCH4V TGCA 8 cut(s) 23, 127, 207, 263, 367, 392, 588, 733
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 398
HpyF3I CTNAG 2 cut(s) 169, 288
HpySE526I ACGT 2 cut(s) 765, 798
Hsp92II CATG 4 cut(s) 109, 236, 380, 737
Kzo9I GATC 7 cut(s) 28, 119, 135, 139, 517, 695, 760
LmnI GCTCC 1 cut(s) 102
Lsp1109I GCAGC 1 cut(s) 717
LweI GCATC 4 cut(s) 139, 250, 376, 405
MaeI CTAG 3 cut(s) 75, 701, 804
MaeII ACGT 2 cut(s) 765, 798
MaeIII GTNAC 3 cut(s) 110, 744, 799
MalI GATC 7 cut(s) 30, 121, 137, 141, 519, 697, 762
MboI GATC 7 cut(s) 28, 119, 135, 139, 517, 695, 760
MboII GAAGA 2 cut(s) 482, 506
MluCI AATT 4 cut(s) 534, 650, 768, 813
MmeI TCCRAC 1 cut(s) 595
MnlI CCTC 6 cut(s) 19, 221, 591, 613, 618, 665
MseI TTAA 4 cut(s) 219, 489, 771, 829
MspCI CTTAAG 1 cut(s) 218
MwoI GCNNNNNNNGC 2 cut(s) 209, 398
NdeII GATC 7 cut(s) 28, 119, 135, 139, 517, 695, 760
NlaIII CATG 4 cut(s) 109, 236, 380, 737
NlaIV GGNNCC 2 cut(s) 69, 608
NmuCI GTSAC 1 cut(s) 744
NspI RCATGY 2 cut(s) 109, 737
PagI TCATGA 1 cut(s) 376
PfeI GAWTC 4 cut(s) 411, 754, 782, 788
PflMI CCANNNNNTGG 1 cut(s) 281
PkrI GCNGC 1 cut(s) 732
Ple19I CGATCG 1 cut(s) 138
Ppu21I YACGTR 1 cut(s) 766
PspN4I GGNNCC 2 cut(s) 69, 608
PspPI GGNCC 3 cut(s) 68, 460, 606
PsrI GAACNNNNNNTAC 2 cut(s) 277, 309
PvuI CGATCG 1 cut(s) 138
RsaI GTAC 2 cut(s) 544, 551
RsaNI GTAC 2 cut(s) 543, 550
SaqAI TTAA 4 cut(s) 219, 489, 771, 829
SatI GCNGC 1 cut(s) 731
Sau3AI GATC 7 cut(s) 28, 119, 135, 139, 517, 695, 760
Sau96I GGNCC 3 cut(s) 68, 460, 606
ScaI AGTACT 1 cut(s) 551
SfaNI GCATC 4 cut(s) 139, 250, 376, 405
SfcI CTRYAG 2 cut(s) 196, 553
SinI GGWCC 2 cut(s) 460, 606
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
SpeI ACTAGT 1 cut(s) 803
Sse9I AATT 4 cut(s) 534, 650, 768, 813
SsiI CCGC 1 cut(s) 658
SspI AATATT 1 cut(s) 826
SspMI CTAG 3 cut(s) 75, 701, 804
TaaI ACNGT 5 cut(s) 59, 383, 631, 744, 776
TaiI ACGT 2 cut(s) 768, 801
TaqI TCGA 2 cut(s) 138, 694
TasI AATT 4 cut(s) 534, 650, 768, 813
TatI WGTACW 1 cut(s) 549
TfiI GAWTC 4 cut(s) 411, 754, 782, 788
Tru1I TTAA 4 cut(s) 219, 489, 771, 829
Tru9I TTAA 4 cut(s) 219, 489, 771, 829
TscAI CASTG 3 cut(s) 64, 317, 753
TseFI GTSAC 1 cut(s) 744
TseI GCWGC 1 cut(s) 730
Tsp45I GTSAC 1 cut(s) 744
TspDTI ATGAA 2 cut(s) 365, 767
TspRI CASTG 3 cut(s) 64, 317, 753
Van91I CCANNNNNTGG 1 cut(s) 281
Vha464I CTTAAG 1 cut(s) 218
VpaK11BI GGWCC 2 cut(s) 460, 606
XapI RAATTY 1 cut(s) 650
XbaI TCTAGA 1 cut(s) 700
XceI RCATGY 2 cut(s) 109, 737
XmiI GTMKAC 1 cut(s) 293
XspI CTAG 3 cut(s) 75, 701, 804
ZrmI AGTACT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.