Rroxscaffold_1G00022170

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006048
Physical Location & Seq
Reverse (-)
27271691 .. 27273819
2129 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00022170.1

Sequence Viewer

Length: 807 bp
ATGATGAACATGATGAAGTTGGGTGCATCTGGATGGAGGGATATAAAAAGATGGTTTAAGTTACTTGGAGAGGATGAGAGGGAGTTTAATTATGACGACAACAGTGAGAAGGGGCCAGCTAGATGGGGAAAGATACGCCATGAATGGAGCATATGTAACAATGGATCGTTGCAGTCTCCCATAGATCTGTTGGATGACAGAGTTGATGTAGTCTCAGATTTAGGGAAACTTCAGGGTAACTACAGGGCTTGCAATGCCACTCTTAAGAATAGAGGCCATGATATGATGTTGAAATGGCAAGCTGATGCAGGATATATTCCAATCAATGGAACTCAGTATAGAGTCAGACAATGCCATTGGCACTCACCTTCTGAACACACCATCAATGGCAAGCAGTTTGATTTAGAGGTGCATCTGGTTCATGACAGTCCAAATGGAAGTATCGCTGTTATCGGGATCCTGTATAAGTTCGGAAAACCTGACCCATTTTTGACACGGATGATGGACTACTTAGAAGACTTATCTGATAGCAGTAAAGAAGAGAAAGTGATTGGTATGGTTGATCCAAAGCAAATAAAAACTGTCAGTACAAAGTACTACAGATATATAGGCTCTCTTACAACTCCTCCTTGCACACAAAATGTCCTCTGGACCCTTGTTGGAGAGGTGAGGACTGTCTCTAAAGAACAGGTCAAATTCCTCCGTAAAGCTGTTCACGATGAGTCAGAAACTAATGCGAGACAAGTACAACCGATAAACAGGCGCTCGGTGAAACTTTATAATCCCAATACACAGGATCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0008150 GO:0010033 GO:0010037 GO:0016020 GO:0042221 GO:0042493 GO:0050896 GO:0072347 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

268

Amino Acids

30.92

Weight (kDa)

8.54

Isoelectric Point (pI)

33.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 38 - 258 2e-42 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 780
AccB7I CCANNNNNTGG 1 cut(s) 326
AclWI GGATC 5 cut(s) 172, 451, 464, 557, 804
AcsI RAATTY 1 cut(s) 695
AcuI CTGAAG 1 cut(s) 215
AfaI GTAC 3 cut(s) 589, 596, 747
AfiI CCNNNNNNNGG 1 cut(s) 326
AflII CTTAAG 1 cut(s) 263
AgsI TTSAA 1 cut(s) 292
AluBI AGCT 3 cut(s) 119, 302, 710
AluI AGCT 3 cut(s) 119, 302, 710
Alw26I GTCTC 4 cut(s) 180, 217, 682, 733
AlwI GGATC 5 cut(s) 172, 451, 464, 557, 804
AoxI GGCC 2 cut(s) 113, 274
ApoI RAATTY 1 cut(s) 695
AspLEI GCGC 1 cut(s) 765
AspS9I GGNCC 2 cut(s) 113, 651
AsuHPI GGTGA 3 cut(s) 357, 679, 781
AvaII GGWCC 1 cut(s) 651
BamHI GGATCC 1 cut(s) 456
BbsI GAAGAC 1 cut(s) 522
BccI CCATC 5 cut(s) 27, 45, 117, 389, 496
BcoDI GTCTC 4 cut(s) 180, 217, 682, 733
BfaI CTAG 1 cut(s) 120
BfmI CTRYAG 2 cut(s) 241, 598
BfoI RGCGCY 1 cut(s) 766
BfrI CTTAAG 1 cut(s) 263
BglII AGATCT 1 cut(s) 184
BmcAI AGTACT 1 cut(s) 596
Bme18I GGWCC 1 cut(s) 651
BmgT120I GGNCC 2 cut(s) 113, 651
BmiI GGNNCC 3 cut(s) 114, 458, 653
BmsI GCATC 3 cut(s) 35, 295, 421
BpiI GAAGAC 1 cut(s) 522
BsaXI ACNNNNNCTCC 4 cut(s) 139, 169, 610, 640
Bsc4I CCNNNNNNNGG 1 cut(s) 326
Bse3DI GCAATG 1 cut(s) 259
BseGI GGATG 4 cut(s) 38, 79, 199, 504
BseLI CCNNNNNNNGG 1 cut(s) 326
BseMI GCAATG 1 cut(s) 259
BseMII CTCAG 2 cut(s) 228, 347
BseRI GAGGAG 1 cut(s) 615
BshFI GGCC 2 cut(s) 115, 276
BslI CCNNNNNNNGG 1 cut(s) 326
BsmAI GTCTC 4 cut(s) 180, 217, 682, 733
BsnI GGCC 2 cut(s) 115, 276
Bsp143I GATC 5 cut(s) 164, 184, 456, 562, 796
BspANI GGCC 2 cut(s) 115, 276
BspCNI CTCAG 2 cut(s) 227, 346
BspHI TCATGA 1 cut(s) 421
BspLI GGNNCC 3 cut(s) 114, 458, 653
BspPI GGATC 5 cut(s) 172, 451, 464, 557, 804
BspTI CTTAAG 1 cut(s) 263
BsrDI GCAATG 1 cut(s) 259
BssMI GATC 5 cut(s) 164, 184, 456, 562, 796
Bst4CI ACNGT 4 cut(s) 104, 428, 583, 676
Bst6I CTCTTC 1 cut(s) 534
BstAFI CTTAAG 1 cut(s) 263
BstC8I GCNNGC 4 cut(s) 117, 250, 300, 392
BstDEI CTNAG 3 cut(s) 214, 333, 511
BstF5I GGATG 4 cut(s) 38, 79, 199, 504
BstH2I RGCGCY 1 cut(s) 766
BstHHI GCGC 1 cut(s) 765
BstKTI GATC 5 cut(s) 167, 187, 459, 565, 799
BstMAI GTCTC 4 cut(s) 180, 217, 682, 733
BstMBI GATC 5 cut(s) 164, 184, 456, 562, 796
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstSFI CTRYAG 2 cut(s) 241, 598
BstV2I GAAGAC 1 cut(s) 522
BstX2I RGATCY 2 cut(s) 184, 456
BstXI CCANNNNNNTGG 1 cut(s) 123
BstYI RGATCY 2 cut(s) 184, 456
BsuRI GGCC 2 cut(s) 115, 276
BtsCI GGATG 4 cut(s) 38, 79, 199, 504
BtsIMutI CAGTG 1 cut(s) 109
Cac8I GCNNGC 4 cut(s) 117, 250, 300, 392
CciI TCATGA 1 cut(s) 421
CfoI GCGC 1 cut(s) 765
Cfr13I GGNCC 2 cut(s) 113, 651
Csp6I GTAC 3 cut(s) 588, 595, 746
CviAII CATG 4 cut(s) 10, 140, 278, 422
CviJI RGCY 7 cut(s) 115, 119, 248, 276, 302, 612, 710
CviKI_1 RGCY 7 cut(s) 115, 119, 248, 276, 302, 612, 710
CviQI GTAC 3 cut(s) 588, 595, 746
DdeI CTNAG 3 cut(s) 214, 333, 511
DpnI GATC 5 cut(s) 166, 186, 458, 564, 798
DpnII GATC 5 cut(s) 164, 184, 456, 562, 796
Eam1104I CTCTTC 1 cut(s) 534
EarI CTCTTC 1 cut(s) 534
Eco47I GGWCC 1 cut(s) 651
Eco57I CTGAAG 1 cut(s) 215
FaeI CATG 4 cut(s) 13, 143, 281, 425
FatI CATG 4 cut(s) 9, 139, 277, 421
FauNDI CATATG 1 cut(s) 152
FokI GGATG 4 cut(s) 45, 86, 206, 511
FspBI CTAG 1 cut(s) 120
GlaI GCGC 1 cut(s) 764
HaeII RGCGCY 1 cut(s) 766
HaeIII GGCC 2 cut(s) 115, 276
HhaI GCGC 1 cut(s) 765
Hin1II CATG 4 cut(s) 13, 143, 281, 425
Hin6I GCGC 1 cut(s) 763
HinP1I GCGC 1 cut(s) 763
HinfI GANTC 2 cut(s) 342, 722
HphI GGTGA 3 cut(s) 357, 679, 781
Hpy166II GTNNAC 1 cut(s) 715
Hpy188I TCNGA 7 cut(s) 217, 347, 373, 473, 526, 727, 801
Hpy188III TCNNGA 5 cut(s) 30, 422, 454, 649, 716
Hpy8I GTNNAC 1 cut(s) 715
HpyAV CCTTC 2 cut(s) 103, 378
HpyCH4III ACNGT 4 cut(s) 104, 428, 583, 676
HpyCH4V TGCA 6 cut(s) 26, 172, 252, 308, 412, 633
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 3 cut(s) 214, 333, 511
Hsp92II CATG 4 cut(s) 13, 143, 281, 425
HspAI GCGC 1 cut(s) 763
Kzo9I GATC 5 cut(s) 164, 184, 456, 562, 796
LmnI GCTCC 1 cut(s) 147
LweI GCATC 3 cut(s) 35, 295, 421
MaeI CTAG 1 cut(s) 120
MaeIII GTNAC 3 cut(s) 60, 155, 236
MalI GATC 5 cut(s) 166, 186, 458, 564, 798
MboI GATC 5 cut(s) 164, 184, 456, 562, 796
MboII GAAGA 2 cut(s) 527, 551
MflI RGATCY 2 cut(s) 184, 456
MluCI AATT 3 cut(s) 88, 695, 802
MlyI GAGTC 2 cut(s) 351, 731
MmeI TCCRAC 2 cut(s) 171, 640
MseI TTAA 3 cut(s) 57, 87, 264
MslI CAYNNNNRTG 1 cut(s) 31
MspCI CTTAAG 1 cut(s) 263
MwoI GCNNNNNNNGC 1 cut(s) 254
NdeI CATATG 1 cut(s) 152
NdeII GATC 5 cut(s) 164, 184, 456, 562, 796
NlaIII CATG 4 cut(s) 13, 143, 281, 425
NlaIV GGNNCC 3 cut(s) 114, 458, 653
PagI TCATGA 1 cut(s) 421
PflMI CCANNNNNTGG 1 cut(s) 326
PleI GAGTC 2 cut(s) 350, 730
PpsI GAGTC 2 cut(s) 350, 730
PsiI TTATAA 1 cut(s) 780
PspN4I GGNNCC 3 cut(s) 114, 458, 653
PspPI GGNCC 2 cut(s) 113, 651
PsuI RGATCY 2 cut(s) 184, 456
RsaI GTAC 3 cut(s) 589, 596, 747
RsaNI GTAC 3 cut(s) 588, 595, 746
RseI CAYNNNNRTG 1 cut(s) 31
SaqAI TTAA 3 cut(s) 57, 87, 264
Sau3AI GATC 5 cut(s) 164, 184, 456, 562, 796
Sau96I GGNCC 2 cut(s) 113, 651
ScaI AGTACT 1 cut(s) 596
SchI GAGTC 2 cut(s) 351, 731
SetI ASST 8 cut(s) 121, 304, 370, 411, 481, 669, 693, 712
SfaNI GCATC 3 cut(s) 35, 295, 421
SfcI CTRYAG 2 cut(s) 241, 598
SinI GGWCC 1 cut(s) 651
SmiMI CAYNNNNRTG 1 cut(s) 31
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 3 cut(s) 88, 695, 802
SspMI CTAG 1 cut(s) 120
TaaI ACNGT 4 cut(s) 104, 428, 583, 676
TasI AATT 3 cut(s) 88, 695, 802
TatI WGTACW 3 cut(s) 587, 594, 745
Tru1I TTAA 3 cut(s) 57, 87, 264
Tru9I TTAA 3 cut(s) 57, 87, 264
TscAI CASTG 1 cut(s) 109
TspDTI ATGAA 4 cut(s) 20, 29, 156, 410
TspGWI ACGGA 2 cut(s) 511, 692
TspRI CASTG 1 cut(s) 109
Van91I CCANNNNNTGG 1 cut(s) 326
Vha464I CTTAAG 1 cut(s) 263
VpaK11BI GGWCC 1 cut(s) 651
XapI RAATTY 1 cut(s) 695
XcmI CCANNNNNNNNNTGG 1 cut(s) 187
XspI CTAG 1 cut(s) 120
ZrmI AGTACT 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.