Rroxscaffold_1G00024250

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006048
Physical Location & Seq
Forward (+)
30156815 .. 30158433
1619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00024250.1

Sequence Viewer

Length: 708 bp
ATGTTGAAGCTACCCAGCCTATTAGTTTTGGTTTGCAGCATTGTTCTTGTTTTGCATCCATACCAAGCAACCTCACATGATGAACATGATGAATTTGAGGATCAGATGGAGTTTAGTTATGACGACAACAGTGAGAAGGGGCCAGCTAGATGGGGAAAGATACGCCATGAATGGAGCATGTGTAACACTGGATCGTTGCAGTCTCCGATCGATCTGTTAGATGACAGAGTTGATGTAGTCTCAGATTTAGGGACACTTCAGGATAGCTACAGGGCTTGCAATGCCACTATTAAGAATAGAGGCCATGATGTGATGTTGAAATGGCAAGCTGATGCAGGATACATTCCAATCAATGGAACTCAGTATACAGTCAGACAATGCCACTGGCACTCACCTTCTGAACACACCATCAATGGCAAGCAGTTTGATTTAGAGGTGCATCTGGTTCATGACAGTCCAAATGCAAGCATCGCTGTTATCGGAATCCTGTATAAGATTGGAAAACCTGACCCATTTTTGACACAGATGATGGACCACTTGGAAGACTTATCTGATAGCAGTAAAGAAGAGAAAGTGATTGGTATGGTTGATCCAAAGCAAATAAAAACTGTGAGGACTGTCTCTAAAGAACAGGTCAAATTCCTCCGCAAAGCTGTTCACGATGATCACGTAATTAATGGTCTTGATTCGAAATCTGGCAACTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0008150 GO:0010033 GO:0010037 GO:0016020 GO:0042221 GO:0042493 GO:0050896 GO:0072347 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

235

Amino Acids

26.56

Weight (kDa)

5.55

Isoelectric Point (pI)

30.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 47 - 200 7.2e-21 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 353
AccI GTMKAC 1 cut(s) 365
AciI CCGC 1 cut(s) 646
AclWI GGATC 3 cut(s) 108, 199, 584
AcsI RAATTY 2 cut(s) 92, 638
AcuI CTGAAG 1 cut(s) 242
AfiI CCNNNNNNNGG 1 cut(s) 353
AgsI TTSAA 2 cut(s) 7, 319
AluBI AGCT 5 cut(s) 10, 146, 267, 329, 653
AluI AGCT 5 cut(s) 10, 146, 267, 329, 653
Alw26I GTCTC 3 cut(s) 207, 244, 625
AlwI GGATC 3 cut(s) 108, 199, 584
AoxI GGCC 2 cut(s) 140, 301
ApeKI GCWGC 1 cut(s) 36
ApoI RAATTY 2 cut(s) 92, 638
AseI ATTAAT 1 cut(s) 675
AspS9I GGNCC 2 cut(s) 140, 532
AsuHPI GGTGA 1 cut(s) 384
AsuII TTCGAA 1 cut(s) 689
AvaII GGWCC 1 cut(s) 532
BbsI GAAGAC 1 cut(s) 549
BbvI GCAGC 1 cut(s) 48
BccI CCATC 4 cut(s) 100, 144, 416, 523
BciVI GTATCC 1 cut(s) 332
BclI TGATCA 1 cut(s) 664
BcoDI GTCTC 3 cut(s) 207, 244, 625
BfaI CTAG 1 cut(s) 147
BfmI CTRYAG 1 cut(s) 268
BfuI GTATCC 1 cut(s) 332
BisI GCNGC 1 cut(s) 37
BlsI GCNGC 1 cut(s) 38
Bme18I GGWCC 1 cut(s) 532
BmgT120I GGNCC 2 cut(s) 140, 532
BmiI GGNNCC 1 cut(s) 141
BmsI GCATC 4 cut(s) 64, 322, 448, 477
BpiI GAAGAC 1 cut(s) 549
Bpu14I TTCGAA 1 cut(s) 689
Bsa29I ATCGAT 1 cut(s) 210
BsaAI YACGTR 1 cut(s) 670
BsaXI ACNNNNNCTCC 2 cut(s) 166, 196
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse1I ACTGG 2 cut(s) 193, 389
Bse3DI GCAATG 1 cut(s) 286
BseCI ATCGAT 1 cut(s) 210
BseGI GGATG 1 cut(s) 55
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMI GCAATG 1 cut(s) 286
BseMII CTCAG 2 cut(s) 255, 374
BseNI ACTGG 2 cut(s) 193, 389
BseXI GCAGC 1 cut(s) 48
BseYI CCCAGC 1 cut(s) 14
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 2 cut(s) 142, 303
BshVI ATCGAT 1 cut(s) 210
BsiEI CGRYCG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 1 cut(s) 353
BsmAI GTCTC 3 cut(s) 207, 244, 625
BsmFI GGGAC 1 cut(s) 265
BsnI GGCC 2 cut(s) 142, 303
Bsp119I TTCGAA 1 cut(s) 689
Bsp143I GATC 6 cut(s) 100, 191, 207, 211, 589, 664
BspACI CCGC 1 cut(s) 646
BspANI GGCC 2 cut(s) 142, 303
BspCNI CTCAG 2 cut(s) 254, 373
BspDI ATCGAT 1 cut(s) 210
BspHI TCATGA 1 cut(s) 448
BspLI GGNNCC 1 cut(s) 141
BspPI GGATC 3 cut(s) 108, 199, 584
BspT104I TTCGAA 1 cut(s) 689
BsrDI GCAATG 1 cut(s) 286
BsrI ACTGG 2 cut(s) 193, 389
BssMI GATC 6 cut(s) 100, 191, 207, 211, 589, 664
BssNAI GTATAC 1 cut(s) 366
Bst1107I GTATAC 1 cut(s) 366
Bst4CI ACNGT 5 cut(s) 131, 370, 455, 610, 619
Bst6I CTCTTC 1 cut(s) 561
BstBAI YACGTR 1 cut(s) 670
BstBI TTCGAA 1 cut(s) 689
BstC8I GCNNGC 5 cut(s) 144, 277, 327, 419, 466
BstDEI CTNAG 2 cut(s) 241, 360
BstF5I GGATG 1 cut(s) 55
BstKTI GATC 6 cut(s) 103, 194, 210, 214, 592, 667
BstMAI GTCTC 3 cut(s) 207, 244, 625
BstMBI GATC 6 cut(s) 100, 191, 207, 211, 589, 664
BstMCI CGRYCG 1 cut(s) 210
BstMWI GCNNNNNNNGC 2 cut(s) 281, 470
BstNSI RCATGY 1 cut(s) 181
BstSFI CTRYAG 1 cut(s) 268
BstV1I GCAGC 1 cut(s) 48
BstV2I GAAGAC 1 cut(s) 549
BstXI CCANNNNNNTGG 1 cut(s) 150
BstZ17I GTATAC 1 cut(s) 366
Bsu15I ATCGAT 1 cut(s) 210
BsuI GTATCC 1 cut(s) 332
BsuRI GGCC 2 cut(s) 142, 303
BsuTUI ATCGAT 1 cut(s) 210
BtgZI GCGATG 1 cut(s) 454
BtsCI GGATG 1 cut(s) 55
BtsIMutI CAGTG 3 cut(s) 136, 186, 382
Cac8I GCNNGC 5 cut(s) 144, 277, 327, 419, 466
CciI TCATGA 1 cut(s) 448
Cfr13I GGNCC 2 cut(s) 140, 532
ClaI ATCGAT 1 cut(s) 210
CviAII CATG 6 cut(s) 77, 86, 167, 178, 305, 449
CviJI RGCY 9 cut(s) 10, 18, 142, 146, 267, 275, 303, 329, 653
CviKI_1 RGCY 9 cut(s) 10, 18, 142, 146, 267, 275, 303, 329, 653
DdeI CTNAG 2 cut(s) 241, 360
DpnI GATC 6 cut(s) 102, 193, 209, 213, 591, 666
DpnII GATC 6 cut(s) 100, 191, 207, 211, 589, 664
Eam1104I CTCTTC 1 cut(s) 561
EarI CTCTTC 1 cut(s) 561
Eco47I GGWCC 1 cut(s) 532
Eco57I CTGAAG 1 cut(s) 242
FaeI CATG 6 cut(s) 80, 89, 170, 181, 308, 452
FaqI GGGAC 1 cut(s) 265
FatI CATG 6 cut(s) 76, 85, 166, 177, 304, 448
FbaI TGATCA 1 cut(s) 664
FblI GTMKAC 1 cut(s) 365
Fnu4HI GCNGC 1 cut(s) 37
FokI GGATG 1 cut(s) 42
Fsp4HI GCNGC 1 cut(s) 37
FspBI CTAG 1 cut(s) 147
GluI GCNGC 1 cut(s) 37
GsaI CCCAGC 1 cut(s) 18
HaeIII GGCC 2 cut(s) 142, 303
Hin1II CATG 6 cut(s) 80, 89, 170, 181, 308, 452
HinfI GANTC 2 cut(s) 483, 686
HphI GGTGA 1 cut(s) 384
Hpy166II GTNNAC 2 cut(s) 366, 658
Hpy188I TCNGA 7 cut(s) 105, 207, 244, 374, 400, 482, 553
Hpy188III TCNNGA 4 cut(s) 260, 449, 659, 683
Hpy8I GTNNAC 2 cut(s) 366, 658
HpyAV CCTTC 2 cut(s) 130, 405
HpyCH4III ACNGT 5 cut(s) 131, 370, 455, 610, 619
HpyCH4IV ACGT 1 cut(s) 669
HpyCH4V TGCA 7 cut(s) 36, 55, 199, 279, 335, 439, 464
HpyF10VI GCNNNNNNNGC 2 cut(s) 281, 470
HpyF3I CTNAG 2 cut(s) 241, 360
HpySE526I ACGT 1 cut(s) 669
Hsp92II CATG 6 cut(s) 80, 89, 170, 181, 308, 452
Ksp22I TGATCA 1 cut(s) 664
Kzo9I GATC 6 cut(s) 100, 191, 207, 211, 589, 664
LmnI GCTCC 1 cut(s) 174
Lsp1109I GCAGC 1 cut(s) 48
LweI GCATC 4 cut(s) 64, 322, 448, 477
MaeI CTAG 1 cut(s) 147
MaeII ACGT 1 cut(s) 669
MaeIII GTNAC 1 cut(s) 182
MalI GATC 6 cut(s) 102, 193, 209, 213, 591, 666
MboI GATC 6 cut(s) 100, 191, 207, 211, 589, 664
MboII GAAGA 2 cut(s) 554, 578
MluCI AATT 3 cut(s) 92, 638, 672
MnlI CCTC 6 cut(s) 82, 91, 293, 427, 606, 653
MseI TTAA 2 cut(s) 291, 675
MwoI GCNNNNNNNGC 2 cut(s) 281, 470
NdeII GATC 6 cut(s) 100, 191, 207, 211, 589, 664
NlaIII CATG 6 cut(s) 80, 89, 170, 181, 308, 452
NlaIV GGNNCC 1 cut(s) 141
NspI RCATGY 1 cut(s) 181
NspV TTCGAA 1 cut(s) 689
PagI TCATGA 1 cut(s) 448
PcsI WCGNNNNNNNCGW 1 cut(s) 666
PfeI GAWTC 2 cut(s) 483, 686
PflMI CCANNNNNTGG 1 cut(s) 353
PkrI GCNGC 1 cut(s) 38
Ple19I CGATCG 1 cut(s) 210
Ppu21I YACGTR 1 cut(s) 670
PshBI ATTAAT 1 cut(s) 675
PspFI CCCAGC 1 cut(s) 14
PspN4I GGNNCC 1 cut(s) 141
PspPI GGNCC 2 cut(s) 140, 532
PsrI GAACNNNNNNTAC 2 cut(s) 349, 381
PvuI CGATCG 1 cut(s) 210
SaqAI TTAA 2 cut(s) 291, 675
SatI GCNGC 1 cut(s) 37
Sau3AI GATC 6 cut(s) 100, 191, 207, 211, 589, 664
Sau96I GGNCC 2 cut(s) 140, 532
SfaNI GCATC 4 cut(s) 64, 322, 448, 477
SfcI CTRYAG 1 cut(s) 268
SfuI TTCGAA 1 cut(s) 689
SinI GGWCC 1 cut(s) 532
Sse9I AATT 3 cut(s) 92, 638, 672
SsiI CCGC 1 cut(s) 646
SspMI CTAG 1 cut(s) 147
TaaI ACNGT 5 cut(s) 131, 370, 455, 610, 619
TaiI ACGT 1 cut(s) 672
TaqI TCGA 2 cut(s) 210, 689
TasI AATT 3 cut(s) 92, 638, 672
TfiI GAWTC 2 cut(s) 483, 686
Tru1I TTAA 2 cut(s) 291, 675
Tru9I TTAA 2 cut(s) 291, 675
TscAI CASTG 3 cut(s) 136, 193, 389
TseI GCWGC 1 cut(s) 36
TspDTI ATGAA 4 cut(s) 96, 105, 183, 437
TspRI CASTG 3 cut(s) 136, 193, 389
Van91I CCANNNNNTGG 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 532
VspI ATTAAT 1 cut(s) 675
XapI RAATTY 2 cut(s) 92, 638
XceI RCATGY 1 cut(s) 181
XmiI GTMKAC 1 cut(s) 365
XspI CTAG 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.