RchiOBHm_Chr5g0058391

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006048
Physical Location & Seq
Forward (+)
62767808 .. 62769275
1468 bp
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UTR
Exon/CDS
Intron
PRQ33505

Sequence Viewer

Length: 780 bp
ATGGTGGGATTTAAAACTGGATTTGTCACTTTAAAATTCCTTGAGGATGAGAGGGAGTTTAATTATGACGACAACAGTGAGAAGGGGCCAGCTAGATGGGGAAAGATACGCCATGAATGGAGCATATGTAACAATGGATCGTTGCAGTCTCCCATAGATCTGTTAGATGACAGAGTTGATGTAGTCTCAGATTTAGGGAAACTTCAGGGTAACTACAGGGCTTGCAATGCCACTCTTAAGAATAGAGGCCATGATATGATGTTGAAATGGCATGCTGATGCAGGATATATTCCAATCAATGGAACTCAGTATACAGTCAGACAATGCCATTGGCACTCACCTTCTGAACACACCATCAATGGCAAGCAGTTTGATTTAGAGGTGCATCTGGTTCATGACAGTCCAAATGGAAGCATCGCTGTTATCGGGATCCTGTATAAGTTTGGAAAACCTGACCCATTTTTGACACGGATGAAGGACTACTTAGAAGACTTATCTGATAGCAGTAAAGAAGAGAAAGTGATTGGTATGGTTGATCCAAAGCAAATAAAAACTGTCAGTACAAAGTACTACAGATATATAGGCTCTCTTACAACTCCTCCTTGCACACAAAATGTCCTCTGGACCCTTGTTGGAGAGGTGAGGACTGTCTCTAAAGAACAGGTCAAATTCCTCCGTAAAGCTGTTCACGATGAGTCAGAAACTAATGCAAGACCAATACAACAGATAAACAGGCGCTCGGTGAAACTTTATAAACCCAGTACACAGGATCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0008150 GO:0010033 GO:0010037 GO:0016020 GO:0042221 GO:0042493 GO:0050896 GO:0072347 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

259

Amino Acids

29.69

Weight (kDa)

8.23

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 29 - 249 1.7e-41 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 753
AccB7I CCANNNNNTGG 1 cut(s) 299
AccI GTMKAC 1 cut(s) 311
AclWI GGATC 5 cut(s) 145, 424, 437, 530, 777
AcsI RAATTY 2 cut(s) 35, 668
AcuI CTGAAG 1 cut(s) 188
AfaI GTAC 3 cut(s) 562, 569, 763
AfiI CCNNNNNNNGG 1 cut(s) 299
AflII CTTAAG 1 cut(s) 236
AgsI TTSAA 1 cut(s) 265
AluBI AGCT 2 cut(s) 92, 683
AluI AGCT 2 cut(s) 92, 683
Alw26I GTCTC 3 cut(s) 153, 190, 655
AlwI GGATC 5 cut(s) 145, 424, 437, 530, 777
AoxI GGCC 2 cut(s) 86, 247
ApoI RAATTY 2 cut(s) 35, 668
AspLEI GCGC 1 cut(s) 738
AspS9I GGNCC 2 cut(s) 86, 624
AsuHPI GGTGA 3 cut(s) 330, 652, 754
AvaII GGWCC 1 cut(s) 624
BamHI GGATCC 1 cut(s) 429
BbsI GAAGAC 1 cut(s) 495
BccI CCATC 2 cut(s) 90, 362
BcoDI GTCTC 3 cut(s) 153, 190, 655
BfaI CTAG 1 cut(s) 93
BfmI CTRYAG 2 cut(s) 214, 571
BfoI RGCGCY 1 cut(s) 739
BfrI CTTAAG 1 cut(s) 236
BglII AGATCT 1 cut(s) 157
BmcAI AGTACT 1 cut(s) 569
Bme18I GGWCC 1 cut(s) 624
BmgT120I GGNCC 2 cut(s) 86, 624
BmiI GGNNCC 3 cut(s) 87, 431, 626
BmrI ACTGGG 1 cut(s) 753
BmsI GCATC 3 cut(s) 268, 394, 423
BmuI ACTGGG 1 cut(s) 753
BpiI GAAGAC 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 62
BsaXI ACNNNNNCTCC 4 cut(s) 112, 142, 583, 613
Bsc4I CCNNNNNNNGG 1 cut(s) 299
Bse1I ACTGG 2 cut(s) 22, 759
Bse3DI GCAATG 1 cut(s) 232
BseGI GGATG 2 cut(s) 52, 477
BseLI CCNNNNNNNGG 1 cut(s) 299
BseMI GCAATG 1 cut(s) 232
BseMII CTCAG 2 cut(s) 201, 320
BseNI ACTGG 2 cut(s) 22, 759
BseRI GAGGAG 1 cut(s) 588
BshFI GGCC 2 cut(s) 88, 249
BslI CCNNNNNNNGG 1 cut(s) 299
BsmAI GTCTC 3 cut(s) 153, 190, 655
BsnI GGCC 2 cut(s) 88, 249
Bsp143I GATC 5 cut(s) 137, 157, 429, 535, 769
BspANI GGCC 2 cut(s) 88, 249
BspCNI CTCAG 2 cut(s) 200, 319
BspHI TCATGA 1 cut(s) 394
BspLI GGNNCC 3 cut(s) 87, 431, 626
BspPI GGATC 5 cut(s) 145, 424, 437, 530, 777
BspTI CTTAAG 1 cut(s) 236
BsrDI GCAATG 1 cut(s) 232
BsrI ACTGG 2 cut(s) 22, 759
BssMI GATC 5 cut(s) 137, 157, 429, 535, 769
BssNAI GTATAC 1 cut(s) 312
Bst1107I GTATAC 1 cut(s) 312
Bst4CI ACNGT 5 cut(s) 77, 316, 401, 556, 649
Bst6I CTCTTC 1 cut(s) 507
BstAFI CTTAAG 1 cut(s) 236
BstC8I GCNNGC 4 cut(s) 90, 223, 273, 365
BstDEI CTNAG 3 cut(s) 187, 306, 484
BstF5I GGATG 2 cut(s) 52, 477
BstH2I RGCGCY 1 cut(s) 739
BstHHI GCGC 1 cut(s) 738
BstKTI GATC 5 cut(s) 140, 160, 432, 538, 772
BstMAI GTCTC 3 cut(s) 153, 190, 655
BstMBI GATC 5 cut(s) 137, 157, 429, 535, 769
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstNSI RCATGY 1 cut(s) 275
BstSFI CTRYAG 2 cut(s) 214, 571
BstV2I GAAGAC 1 cut(s) 495
BstX2I RGATCY 2 cut(s) 157, 429
BstXI CCANNNNNNTGG 1 cut(s) 96
BstYI RGATCY 2 cut(s) 157, 429
BstZ17I GTATAC 1 cut(s) 312
BsuRI GGCC 2 cut(s) 88, 249
BtgZI GCGATG 1 cut(s) 400
BtsCI GGATG 2 cut(s) 52, 477
BtsIMutI CAGTG 1 cut(s) 82
Cac8I GCNNGC 4 cut(s) 90, 223, 273, 365
CciI TCATGA 1 cut(s) 394
CfoI GCGC 1 cut(s) 738
Cfr13I GGNCC 2 cut(s) 86, 624
Csp6I GTAC 3 cut(s) 561, 568, 762
CviAII CATG 4 cut(s) 113, 251, 272, 395
CviJI RGCY 6 cut(s) 88, 92, 221, 249, 585, 683
CviKI_1 RGCY 6 cut(s) 88, 92, 221, 249, 585, 683
CviQI GTAC 3 cut(s) 561, 568, 762
DdeI CTNAG 3 cut(s) 187, 306, 484
DpnI GATC 5 cut(s) 139, 159, 431, 537, 771
DpnII GATC 5 cut(s) 137, 157, 429, 535, 769
DraI TTTAAA 2 cut(s) 13, 33
Eam1104I CTCTTC 1 cut(s) 507
EarI CTCTTC 1 cut(s) 507
Eco47I GGWCC 1 cut(s) 624
Eco57I CTGAAG 1 cut(s) 188
FaeI CATG 4 cut(s) 116, 254, 275, 398
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FatI CATG 4 cut(s) 112, 250, 271, 394
FauNDI CATATG 1 cut(s) 125
FblI GTMKAC 1 cut(s) 311
FokI GGATG 2 cut(s) 59, 484
FspBI CTAG 1 cut(s) 93
GlaI GCGC 1 cut(s) 737
HaeII RGCGCY 1 cut(s) 739
HaeIII GGCC 2 cut(s) 88, 249
HhaI GCGC 1 cut(s) 738
Hin1II CATG 4 cut(s) 116, 254, 275, 398
Hin6I GCGC 1 cut(s) 736
HinP1I GCGC 1 cut(s) 736
HinfI GANTC 1 cut(s) 695
HphI GGTGA 3 cut(s) 330, 652, 754
Hpy166II GTNNAC 3 cut(s) 312, 688, 764
Hpy188I TCNGA 6 cut(s) 190, 320, 346, 499, 700, 774
Hpy188III TCNNGA 4 cut(s) 395, 427, 622, 689
Hpy8I GTNNAC 3 cut(s) 312, 688, 764
HpyAV CCTTC 3 cut(s) 76, 351, 469
HpyCH4III ACNGT 5 cut(s) 77, 316, 401, 556, 649
HpyCH4V TGCA 6 cut(s) 145, 225, 281, 385, 606, 710
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpyF3I CTNAG 3 cut(s) 187, 306, 484
Hsp92II CATG 4 cut(s) 116, 254, 275, 398
HspAI GCGC 1 cut(s) 736
Kzo9I GATC 5 cut(s) 137, 157, 429, 535, 769
LmnI GCTCC 1 cut(s) 120
LweI GCATC 3 cut(s) 268, 394, 423
MaeI CTAG 1 cut(s) 93
MaeIII GTNAC 3 cut(s) 25, 128, 209
MalI GATC 5 cut(s) 139, 159, 431, 537, 771
MboI GATC 5 cut(s) 137, 157, 429, 535, 769
MboII GAAGA 2 cut(s) 500, 524
MflI RGATCY 2 cut(s) 157, 429
MluCI AATT 4 cut(s) 35, 61, 668, 775
MlyI GAGTC 1 cut(s) 704
MmeI TCCRAC 1 cut(s) 613
MnlI CCTC 9 cut(s) 37, 45, 239, 373, 609, 629, 631, 636, 683
MseI TTAA 4 cut(s) 12, 32, 60, 237
MslI CAYNNNNRTG 1 cut(s) 276
MspCI CTTAAG 1 cut(s) 236
MwoI GCNNNNNNNGC 1 cut(s) 227
NdeI CATATG 1 cut(s) 125
NdeII GATC 5 cut(s) 137, 157, 429, 535, 769
NlaIII CATG 4 cut(s) 116, 254, 275, 398
NlaIV GGNNCC 3 cut(s) 87, 431, 626
NmuCI GTSAC 1 cut(s) 25
NspI RCATGY 1 cut(s) 275
PaeI GCATGC 1 cut(s) 275
PagI TCATGA 1 cut(s) 394
PflMI CCANNNNNTGG 1 cut(s) 299
PleI GAGTC 1 cut(s) 703
PpsI GAGTC 1 cut(s) 703
PsiI TTATAA 1 cut(s) 753
PspN4I GGNNCC 3 cut(s) 87, 431, 626
PspPI GGNCC 2 cut(s) 86, 624
PsrI GAACNNNNNNTAC 2 cut(s) 295, 327
PsuI RGATCY 2 cut(s) 157, 429
RsaI GTAC 3 cut(s) 562, 569, 763
RsaNI GTAC 3 cut(s) 561, 568, 762
RseI CAYNNNNRTG 1 cut(s) 276
SaqAI TTAA 4 cut(s) 12, 32, 60, 237
Sau3AI GATC 5 cut(s) 137, 157, 429, 535, 769
Sau96I GGNCC 2 cut(s) 86, 624
ScaI AGTACT 1 cut(s) 569
SchI GAGTC 1 cut(s) 704
SetI ASST 7 cut(s) 94, 343, 384, 454, 642, 666, 685
SfaNI GCATC 3 cut(s) 268, 394, 423
SfcI CTRYAG 2 cut(s) 214, 571
SinI GGWCC 1 cut(s) 624
SmiMI CAYNNNNRTG 1 cut(s) 276
SmlI CTYRAG 2 cut(s) 41, 236
SmoI CTYRAG 2 cut(s) 41, 236
SphI GCATGC 1 cut(s) 275
Sse9I AATT 4 cut(s) 35, 61, 668, 775
SspMI CTAG 1 cut(s) 93
TaaI ACNGT 5 cut(s) 77, 316, 401, 556, 649
TasI AATT 4 cut(s) 35, 61, 668, 775
TatI WGTACW 3 cut(s) 560, 567, 761
Tru1I TTAA 4 cut(s) 12, 32, 60, 237
Tru9I TTAA 4 cut(s) 12, 32, 60, 237
TscAI CASTG 1 cut(s) 82
TseFI GTSAC 1 cut(s) 25
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 3 cut(s) 129, 383, 488
TspGWI ACGGA 2 cut(s) 484, 665
TspRI CASTG 1 cut(s) 82
Van91I CCANNNNNTGG 1 cut(s) 299
Vha464I CTTAAG 1 cut(s) 236
VpaK11BI GGWCC 1 cut(s) 624
XapI RAATTY 2 cut(s) 35, 668
XceI RCATGY 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 311
XspI CTAG 1 cut(s) 93
ZrmI AGTACT 1 cut(s) 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.