AT2G28500

lob domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004073
Physical Location & Seq
Reverse (-)
12186274 .. 12188293
2020 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G28500.1

Sequence Viewer

Length: 699 bp
ATGCTAAAGATGGAGATTAACGGTGGGGTCGCTACACCTACTGCTTCCGCCGTCGCCAAGGTGACAGAAACCACCACTCCCGTTAACTCTCCTTCTCCCACTTCTTCACCTCCGCCGCCTCCTTCGCCGCAACAGCCGCCACAACCACCGGTTGTACTAAGCCCTTGTGCGGCTTGCAAGATTTTGCGGCGGCGGTGCGCCGATAAATGTGTTTTGGCACCGTACTTTCCTCCTACGGACCCGGCGAAGTTCACAATCGCTCACCGTGTCTTTGGAGCTAGCAACATTATTAAGTTCTTGCAGGAACTTCCAGAATCGCAAAGAACAGATGCTGTTAATAGCATGGTCTATGAAGCCGGAGCTAGGATGAGAGATCCGGTTTACGGATGTGCGGGTGCAATTTATCATTTGCAGAGACAAGTGAGTGAGCTTCAAGCACAACTTGCAAAAACTCAAGTAGAGTTAGTAGGCATGCAACTCCAAAGATCAAGTCTACTAGAATTGATATATAATATGGAGCAAACAAAGTTGTCAGTACAAGAGCAAGGACAACAGAAGATGTCCTTTGAGAGTTCATTTGAAAGCGGCGATGAGTTCATTAGTAGCCCGGACGAAGAGAGCAATGATTTGGGGTTCCTTGAGGACAACAACAACAACAATAATTCATCAATGTCGTGGTGGGATCCTCTTTGGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

232

Amino Acids

25.53

Weight (kDa)

4.92

Isoelectric Point (pI)

74.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 55 - 152 1e-40 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 217
AccI GTMKAC 1 cut(s) 493
AclWI GGATC 3 cut(s) 368, 677, 690
AfaI GTAC 3 cut(s) 156, 224, 537
AfiI CCNNNNNNNGG 3 cut(s) 169, 363, 383
AgeI ACCGGT 1 cut(s) 148
AgsI TTSAA 2 cut(s) 434, 581
AluBI AGCT 3 cut(s) 278, 362, 430
AluI AGCT 3 cut(s) 278, 362, 430
Alw26I GTCTC 1 cut(s) 409
AlwI GGATC 3 cut(s) 368, 677, 690
AlwNI CAGNNNCTG 1 cut(s) 332
ArsI GACNNNNNNTTYG 2 cut(s) 475, 507
AsiGI ACCGGT 1 cut(s) 148
AspLEI GCGC 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 238
AsuC2I CCSGG 2 cut(s) 242, 608
AsuHPI GGTGA 3 cut(s) 73, 99, 254
AsuNHI GCTAGC 1 cut(s) 278
AvaII GGWCC 1 cut(s) 238
BamHI GGATCC 1 cut(s) 682
BanI GGYRCC 1 cut(s) 217
BccI CCATC 1 cut(s) 4
BceAI ACGGC 1 cut(s) 35
BcnI CCSGG 2 cut(s) 242, 608
BcoDI GTCTC 1 cut(s) 409
BfaI CTAG 3 cut(s) 279, 363, 497
BisI GCNGC 7 cut(s) 116, 128, 137, 171, 188, 191, 586
BlsI GCNGC 7 cut(s) 117, 129, 138, 172, 189, 192, 587
Bme1390I CCNGG 2 cut(s) 242, 608
Bme18I GGWCC 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 238
BmiI GGNNCC 4 cut(s) 219, 240, 635, 684
BmrFI CCNGG 2 cut(s) 242, 608
BmsI GCATC 1 cut(s) 319
BmtI GCTAGC 1 cut(s) 282
BpuEI CTTGAG 2 cut(s) 438, 659
BpuMI CCSGG 2 cut(s) 242, 608
BsaJI CCNNGG 1 cut(s) 57
BsaWI WCCGGW 2 cut(s) 148, 376
BsaXI ACNNNNNCTCC 2 cut(s) 61, 91
Bsc4I CCNNNNNNNGG 3 cut(s) 169, 363, 383
Bse118I RCCGGY 1 cut(s) 148
Bse3DI GCAATG 1 cut(s) 628
BseDI CCNNGG 1 cut(s) 57
BseGI GGATG 2 cut(s) 372, 392
BseLI CCNNNNNNNGG 3 cut(s) 169, 363, 383
BseMI GCAATG 1 cut(s) 628
BshNI GGYRCC 1 cut(s) 217
BshTI ACCGGT 1 cut(s) 148
BsiSI CCGG 5 cut(s) 149, 242, 357, 377, 608
BslI CCNNNNNNNGG 3 cut(s) 169, 363, 383
BsmAI GTCTC 1 cut(s) 409
Bsp143I GATC 3 cut(s) 373, 485, 682
BspLI GGNNCC 4 cut(s) 219, 240, 635, 684
BspOI GCTAGC 1 cut(s) 282
BspPI GGATC 3 cut(s) 368, 677, 690
BspT107I GGYRCC 1 cut(s) 217
BsrDI GCAATG 1 cut(s) 628
BsrFI RCCGGY 1 cut(s) 148
BssAI RCCGGY 1 cut(s) 148
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 3 cut(s) 373, 485, 682
BssT1I CCWWGG 1 cut(s) 57
Bst4CI ACNGT 3 cut(s) 23, 222, 266
Bst6I CTCTTC 1 cut(s) 609
BstAPI GCANNNNNTGC 1 cut(s) 443
BstC8I GCNNGC 3 cut(s) 175, 280, 473
BstDEI CTNAG 1 cut(s) 158
BstF5I GGATG 2 cut(s) 372, 392
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 3 cut(s) 376, 488, 685
BstMAI GTCTC 1 cut(s) 409
BstMBI GATC 3 cut(s) 373, 485, 682
BstMWI GCNNNNNNNGC 4 cut(s) 124, 133, 136, 443
BstNSI RCATGY 1 cut(s) 475
BstSCI CCNGG 2 cut(s) 240, 606
BstX2I RGATCY 2 cut(s) 373, 682
BstYI RGATCY 2 cut(s) 373, 682
BtgZI GCGATG 1 cut(s) 603
BtsCI GGATG 2 cut(s) 372, 392
Cac8I GCNNGC 3 cut(s) 175, 280, 473
CaiI CAGNNNCTG 1 cut(s) 332
CfoI GCGC 1 cut(s) 200
Cfr10I RCCGGY 1 cut(s) 148
Cfr13I GGNCC 1 cut(s) 238
Csp6I GTAC 3 cut(s) 155, 223, 536
CspAI ACCGGT 1 cut(s) 148
CviAII CATG 3 cut(s) 343, 472, 696
CviJI RGCY 8 cut(s) 136, 162, 173, 278, 356, 362, 430, 606
CviKI_1 RGCY 8 cut(s) 136, 162, 173, 278, 356, 362, 430, 606
CviQI GTAC 3 cut(s) 155, 223, 536
DdeI CTNAG 1 cut(s) 158
DpnI GATC 3 cut(s) 375, 487, 684
DpnII GATC 3 cut(s) 373, 485, 682
Eam1104I CTCTTC 1 cut(s) 609
EarI CTCTTC 1 cut(s) 609
EciI GGCGGA 2 cut(s) 37, 102
Eco130I CCWWGG 1 cut(s) 57
Eco47I GGWCC 1 cut(s) 238
EcoT14I CCWWGG 1 cut(s) 57
ErhI CCWWGG 1 cut(s) 57
FaeI CATG 3 cut(s) 346, 475, 699
FaiI YATR 7 cut(s) 344, 351, 473, 508, 510, 515, 697
FalI AAGNNNNNCTT 4 cut(s) 426, 458, 548, 580
FatI CATG 3 cut(s) 342, 471, 695
FauI CCCGC 1 cut(s) 385
FblI GTMKAC 1 cut(s) 493
Fnu4HI GCNGC 7 cut(s) 116, 128, 137, 171, 188, 191, 586
FokI GGATG 2 cut(s) 379, 399
Fsp4HI GCNGC 7 cut(s) 116, 128, 137, 171, 188, 191, 586
FspBI CTAG 3 cut(s) 279, 363, 497
GlaI GCGC 1 cut(s) 199
GluI GCNGC 7 cut(s) 116, 128, 137, 171, 188, 191, 586
HapII CCGG 5 cut(s) 149, 242, 357, 377, 608
HhaI GCGC 1 cut(s) 200
Hin1II CATG 3 cut(s) 346, 475, 699
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HinfI GANTC 1 cut(s) 314
HpaI GTTAAC 1 cut(s) 85
HpaII CCGG 5 cut(s) 149, 242, 357, 377, 608
HphI GGTGA 3 cut(s) 73, 99, 254
Hpy166II GTNNAC 4 cut(s) 85, 252, 382, 494
Hpy188III TCNNGA 1 cut(s) 311
Hpy8I GTNNAC 4 cut(s) 85, 252, 382, 494
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 2 cut(s) 102, 132
HpyCH4III ACNGT 3 cut(s) 23, 222, 266
HpyCH4V TGCA 6 cut(s) 177, 301, 398, 412, 446, 475
HpyF10VI GCNNNNNNNGC 4 cut(s) 124, 133, 136, 443
HpyF3I CTNAG 1 cut(s) 158
Hsp92II CATG 3 cut(s) 346, 475, 699
HspAI GCGC 1 cut(s) 198
KspAI GTTAAC 1 cut(s) 85
Kzo9I GATC 3 cut(s) 373, 485, 682
LmnI GCTCC 3 cut(s) 275, 359, 517
LpnPI CCDG 7 cut(s) 162, 255, 287, 324, 370, 390, 621
LweI GCATC 1 cut(s) 319
MaeI CTAG 3 cut(s) 279, 363, 497
MaeIII GTNAC 1 cut(s) 61
MalI GATC 3 cut(s) 375, 487, 684
MboI GATC 3 cut(s) 373, 485, 682
MboII GAAGA 3 cut(s) 96, 568, 626
MflI RGATCY 2 cut(s) 373, 682
MluCI AATT 3 cut(s) 399, 500, 661
MnlI CCTC 5 cut(s) 120, 129, 240, 634, 696
MseI TTAA 4 cut(s) 18, 84, 291, 336
MspI CCGG 5 cut(s) 149, 242, 357, 377, 608
MspR9I CCNGG 2 cut(s) 242, 608
MwoI GCNNNNNNNGC 4 cut(s) 124, 133, 136, 443
NciI CCSGG 2 cut(s) 242, 608
NdeII GATC 3 cut(s) 373, 485, 682
NheI GCTAGC 1 cut(s) 278
NlaIII CATG 3 cut(s) 346, 475, 699
NlaIV GGNNCC 4 cut(s) 219, 240, 635, 684
NmuCI GTSAC 1 cut(s) 61
NspI RCATGY 1 cut(s) 475
PaeI GCATGC 1 cut(s) 475
PcsI WCGNNNNNNNCGW 1 cut(s) 242
PfeI GAWTC 1 cut(s) 314
PinAI ACCGGT 1 cut(s) 148
PkrI GCNGC 7 cut(s) 117, 129, 138, 172, 189, 192, 587
PspN4I GGNNCC 4 cut(s) 219, 240, 635, 684
PspPI GGNCC 1 cut(s) 238
PstNI CAGNNNCTG 1 cut(s) 332
PsuI RGATCY 2 cut(s) 373, 682
RsaI GTAC 3 cut(s) 156, 224, 537
RsaNI GTAC 3 cut(s) 155, 223, 536
SaqAI TTAA 4 cut(s) 18, 84, 291, 336
SatI GCNGC 7 cut(s) 116, 128, 137, 171, 188, 191, 586
Sau3AI GATC 3 cut(s) 373, 485, 682
Sau96I GGNCC 1 cut(s) 238
ScrFI CCNGG 2 cut(s) 242, 608
SetI ASST 6 cut(s) 40, 63, 112, 280, 364, 432
SfaNI GCATC 1 cut(s) 319
SinI GGWCC 1 cut(s) 238
SmlI CTYRAG 2 cut(s) 453, 638
SmoI CTYRAG 2 cut(s) 453, 638
SphI GCATGC 1 cut(s) 475
Sse9I AATT 3 cut(s) 399, 500, 661
SspMI CTAG 3 cut(s) 279, 363, 497
StyD4I CCNGG 2 cut(s) 240, 606
StyI CCWWGG 1 cut(s) 57
TaaI ACNGT 3 cut(s) 23, 222, 266
TasI AATT 3 cut(s) 399, 500, 661
TatI WGTACW 2 cut(s) 154, 535
TauI GCSGC 7 cut(s) 118, 130, 139, 173, 190, 193, 588
TfiI GAWTC 1 cut(s) 314
Tru1I TTAA 4 cut(s) 18, 84, 291, 336
Tru9I TTAA 4 cut(s) 18, 84, 291, 336
TseFI GTSAC 1 cut(s) 61
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 4 cut(s) 366, 564, 586, 654
TspGWI ACGGA 2 cut(s) 251, 399
VpaK11BI GGWCC 1 cut(s) 238
XceI RCATGY 1 cut(s) 475
XmiI GTMKAC 1 cut(s) 493
XspI CTAG 3 cut(s) 279, 363, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.