FvH4_7g20130

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004073
Physical Location & Seq
Reverse (-)
16425315 .. 16427138
1824 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g20130.t1

Sequence Viewer

Length: 675 bp
ATGGAATGCAATGGCAACACTGCTGCTACTGCTACTACTACCACACCACATTCAAGTTTCACTTGCTCTCCATTGACATCTTCATCTTCTTCTTCTCCACTGATATCTTCTTCTTCTCCTGCTACCTCTCCTATGCCGCCGCCAGCTGTCGTTGTTAGCCCATGCGCTGCCTGCAAGATCCTTCGACGCCGATGTGTGGAGAGGTGTGTTTTAGCTCCATATTTCCCTCCTTCGGATCCTATCAAGTTCATTATTGCCCATAGAGTCTTTGGGGCCAGCAACATAATCAAGTTCTTGCAGGACCTTCCAGAATCTCAGAGAGCAGATGCAGTGAGCAGCATGGTTTATGAAGCCAATGCCAGGCTTCGAGACCCGGTGTACGGCTGTGCCGGAGCAATTTGCAATCTTCAGAAACAAGTCACTGAGCTCCAATCCCAACTAGCCAAGGCTCAAGCGGAGATCATCAACTTGCAATGCCAGCAAGGCAATTTGATTGCCCTAATCTGCATGGACATGAGTAGTACTACTGCTACCAACACACAGCAATCTAAAGAAGCAGTAGTAGTACCCATTTTGCAGCAGCAACCATACTCGAATTTGAATATCGATACGAGTTGCTTTCTTGATGAGAACAATCTGGGCACTGCTTGGGACTGGGAACCTCTTTGGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

225

Amino Acids

24.19

Weight (kDa)

5.35

Isoelectric Point (pI)

74.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 54 - 151 3e-40 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 137, 140, 455
AclWI GGATC 3 cut(s) 172, 230, 243
AcsI RAATTY 1 cut(s) 595
AcuI CTGAAG 1 cut(s) 392
AcyI GRCGYC 1 cut(s) 187
AfaI GTAC 3 cut(s) 380, 523, 567
AfiI CCNNNNNNNGG 4 cut(s) 196, 232, 360, 380
AgsI TTSAA 2 cut(s) 54, 601
AjiI CACGTC 1 cut(s) 672
AjnI CCWGG 1 cut(s) 359
AluBI AGCT 3 cut(s) 146, 215, 427
AluI AGCT 3 cut(s) 146, 215, 427
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 3 cut(s) 172, 230, 243
AoxI GGCC 1 cut(s) 273
ApeKI GCWGC 5 cut(s) 23, 167, 336, 577, 580
ApoI RAATTY 1 cut(s) 595
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 2 cut(s) 273, 301
AsuC2I CCSGG 1 cut(s) 374
AvaII GGWCC 1 cut(s) 301
BaeGI GKGCMC 1 cut(s) 644
BamHI GGATCC 1 cut(s) 235
BanII GRGCYC 1 cut(s) 429
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 5 cut(s) 10, 154, 348, 589, 592
BceAI ACGGC 1 cut(s) 397
BciT130I CCWGG 1 cut(s) 361
BcnI CCSGG 1 cut(s) 374
BcoDI GTCTC 1 cut(s) 363
BfaI CTAG 1 cut(s) 440
BglI GCCNNNNNGGC 1 cut(s) 483
BisI GCNGC 7 cut(s) 24, 137, 140, 168, 337, 578, 581
BlsI GCNGC 7 cut(s) 25, 138, 141, 169, 338, 579, 582
BmcAI AGTACT 1 cut(s) 523
Bme1390I CCNGG 2 cut(s) 361, 374
Bme18I GGWCC 1 cut(s) 301
BmgBI CACGTC 1 cut(s) 672
BmgT120I GGNCC 2 cut(s) 273, 301
BmiI GGNNCC 3 cut(s) 237, 274, 660
BmrFI CCNGG 2 cut(s) 361, 374
BmrI ACTGGG 1 cut(s) 664
BmsI GCATC 1 cut(s) 316
BmuI ACTGGG 1 cut(s) 664
BpuEI CTTGAG 1 cut(s) 435
BpuMI CCSGG 1 cut(s) 374
Bsa29I ATCGAT 1 cut(s) 606
BsaHI GRCGYC 1 cut(s) 187
BsaI GGTCTC 1 cut(s) 363
BsaJI CCNNGG 1 cut(s) 444
BsaXI ACNNNNNCTCC 2 cut(s) 52, 82
Bsc4I CCNNNNNNNGG 4 cut(s) 196, 232, 360, 380
Bse1I ACTGG 1 cut(s) 659
Bse3DI GCAATG 2 cut(s) 16, 479
BseBI CCWGG 1 cut(s) 361
BseCI ATCGAT 1 cut(s) 606
BseDI CCNNGG 1 cut(s) 444
BseLI CCNNNNNNNGG 4 cut(s) 196, 232, 360, 380
BseMI GCAATG 2 cut(s) 16, 479
BseMII CTCAG 2 cut(s) 329, 414
BseNI ACTGG 1 cut(s) 659
BseSI GKGCMC 1 cut(s) 644
BseXI GCAGC 5 cut(s) 10, 154, 348, 589, 592
BshFI GGCC 1 cut(s) 275
BshVI ATCGAT 1 cut(s) 606
BsiHKAI GWGCWC 1 cut(s) 429
BsiSI CCGG 2 cut(s) 374, 390
BslFI GGGAC 1 cut(s) 665
BslI CCNNNNNNNGG 4 cut(s) 196, 232, 360, 380
BsmAI GTCTC 1 cut(s) 363
BsmFI GGGAC 1 cut(s) 665
BsmI GAATGC 1 cut(s) 11
BsnI GGCC 1 cut(s) 275
Bso31I GGTCTC 1 cut(s) 363
Bsp1286I GDGCHC 2 cut(s) 429, 644
Bsp143I GATC 3 cut(s) 177, 235, 459
BspACI CCGC 3 cut(s) 137, 140, 455
BspANI GGCC 1 cut(s) 275
BspCNI CTCAG 2 cut(s) 328, 415
BspDI ATCGAT 1 cut(s) 606
BspLI GGNNCC 3 cut(s) 237, 274, 660
BspPI GGATC 3 cut(s) 172, 230, 243
BspTNI GGTCTC 1 cut(s) 363
BsrDI GCAATG 2 cut(s) 16, 479
BsrI ACTGG 1 cut(s) 659
BssECI CCNNGG 1 cut(s) 444
BssMI GATC 3 cut(s) 177, 235, 459
BssNI GRCGYC 1 cut(s) 187
BssT1I CCWWGG 1 cut(s) 444
Bst2UI CCWGG 1 cut(s) 361
BstACI GRCGYC 1 cut(s) 187
BstC8I GCNNGC 4 cut(s) 144, 172, 277, 479
BstDEI CTNAG 2 cut(s) 315, 423
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 3 cut(s) 180, 238, 462
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 3 cut(s) 177, 235, 459
BstMWI GCNNNNNNNGC 4 cut(s) 29, 171, 478, 483
BstNI CCWGG 1 cut(s) 361
BstSCI CCNGG 2 cut(s) 359, 372
BstSLI GKGCMC 1 cut(s) 644
BstV1I GCAGC 5 cut(s) 10, 154, 348, 589, 592
BstX2I RGATCY 2 cut(s) 177, 235
BstYI RGATCY 2 cut(s) 177, 235
Bsu15I ATCGAT 1 cut(s) 606
BsuRI GGCC 1 cut(s) 275
BsuTUI ATCGAT 1 cut(s) 606
BtrI CACGTC 1 cut(s) 672
BtsI GCAGTG 3 cut(s) 18, 336, 642
BtsIMutI CAGTG 5 cut(s) 18, 98, 336, 420, 642
Cac8I GCNNGC 4 cut(s) 144, 172, 277, 479
CfoI GCGC 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 273, 301
ClaI ATCGAT 1 cut(s) 606
CseI GACGC 1 cut(s) 195
Csp6I GTAC 3 cut(s) 379, 522, 566
CviAII CATG 4 cut(s) 162, 340, 508, 514
CviQI GTAC 3 cut(s) 379, 522, 566
DdeI CTNAG 2 cut(s) 315, 423
DpnI GATC 3 cut(s) 179, 237, 461
DpnII GATC 3 cut(s) 177, 235, 459
Ecl136II GAGCTC 1 cut(s) 427
Eco130I CCWWGG 1 cut(s) 444
Eco24I GRGCYC 1 cut(s) 429
Eco31I GGTCTC 1 cut(s) 363
Eco32I GATATC 1 cut(s) 105
Eco47I GGWCC 1 cut(s) 301
Eco53kI GAGCTC 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 427
EcoO109I RGGNCCY 1 cut(s) 301
EcoRII CCWGG 1 cut(s) 359
EcoRV GATATC 1 cut(s) 105
EcoT14I CCWWGG 1 cut(s) 444
EcoT38I GRGCYC 1 cut(s) 429
ErhI CCWWGG 1 cut(s) 444
FaeI CATG 4 cut(s) 165, 343, 511, 517
FalI AAGNNNNNCTT 2 cut(s) 46, 78
FaqI GGGAC 1 cut(s) 665
FatI CATG 4 cut(s) 161, 339, 507, 513
Fnu4HI GCNGC 7 cut(s) 24, 137, 140, 168, 337, 578, 581
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 7 cut(s) 24, 137, 140, 168, 337, 578, 581
FspBI CTAG 1 cut(s) 440
GlaI GCGC 1 cut(s) 166
GluI GCNGC 7 cut(s) 24, 137, 140, 168, 337, 578, 581
HaeIII GGCC 1 cut(s) 275
HapII CCGG 2 cut(s) 374, 390
HgaI GACGC 1 cut(s) 195
HhaI GCGC 1 cut(s) 167
Hin1I GRCGYC 1 cut(s) 187
Hin1II CATG 4 cut(s) 165, 343, 511, 517
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HinfI GANTC 2 cut(s) 264, 311
HpaII CCGG 2 cut(s) 374, 390
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 3 cut(s) 235, 318, 411
Hpy188III TCNNGA 3 cut(s) 308, 368, 623
Hpy8I GTNNAC 1 cut(s) 379
Hpy99I CGWCG 1 cut(s) 189
HpyAV CCTTC 3 cut(s) 191, 240, 314
HpyCH4IV ACGT 1 cut(s) 671
HpyCH4V TGCA 8 cut(s) 9, 174, 298, 329, 402, 472, 507, 577
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 171, 478, 483
HpyF3I CTNAG 2 cut(s) 315, 423
HpySE526I ACGT 1 cut(s) 671
Hsp92I GRCGYC 1 cut(s) 187
Hsp92II CATG 4 cut(s) 165, 343, 511, 517
HspAI GCGC 1 cut(s) 165
Kzo9I GATC 3 cut(s) 177, 235, 459
LmnI GCTCC 3 cut(s) 220, 392, 432
Lsp1109I GCAGC 5 cut(s) 10, 154, 348, 589, 592
LweI GCATC 1 cut(s) 316
MaeI CTAG 1 cut(s) 440
MaeII ACGT 1 cut(s) 671
MaeIII GTNAC 1 cut(s) 418
MalI GATC 3 cut(s) 179, 237, 461
MboI GATC 3 cut(s) 177, 235, 459
MboII GAAGA 8 cut(s) 72, 78, 81, 84, 99, 102, 105, 398
MflI RGATCY 2 cut(s) 177, 235
MhlI GDGCHC 2 cut(s) 429, 644
MluCI AATT 3 cut(s) 396, 487, 595
MlyI GAGTC 1 cut(s) 273
MnlI CCTC 4 cut(s) 136, 195, 237, 672
MslI CAYNNNNRTG 1 cut(s) 512
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 2 cut(s) 374, 390
MspR9I CCNGG 2 cut(s) 361, 374
Mva1269I GAATGC 1 cut(s) 11
MvaI CCWGG 1 cut(s) 361
MwoI GCNNNNNNNGC 4 cut(s) 29, 171, 478, 483
NciI CCSGG 1 cut(s) 374
NdeII GATC 3 cut(s) 177, 235, 459
NlaIII CATG 4 cut(s) 165, 343, 511, 517
NlaIV GGNNCC 3 cut(s) 237, 274, 660
NmuCI GTSAC 1 cut(s) 418
PctI GAATGC 1 cut(s) 11
PfeI GAWTC 1 cut(s) 311
PkrI GCNGC 7 cut(s) 25, 138, 141, 169, 338, 579, 582
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PpuMI RGGWCCY 1 cut(s) 301
Psp124BI GAGCTC 1 cut(s) 429
Psp5II RGGWCCY 1 cut(s) 301
Psp6I CCWGG 1 cut(s) 359
PspGI CCWGG 1 cut(s) 359
PspN4I GGNNCC 3 cut(s) 237, 274, 660
PspPI GGNCC 2 cut(s) 273, 301
PspPPI RGGWCCY 1 cut(s) 301
PsuI RGATCY 2 cut(s) 177, 235
PvuII CAGCTG 1 cut(s) 146
RsaI GTAC 3 cut(s) 380, 523, 567
RsaNI GTAC 3 cut(s) 379, 522, 566
RseI CAYNNNNRTG 1 cut(s) 512
SacI GAGCTC 1 cut(s) 429
SatI GCNGC 7 cut(s) 24, 137, 140, 168, 337, 578, 581
Sau3AI GATC 3 cut(s) 177, 235, 459
Sau96I GGNCC 2 cut(s) 273, 301
ScaI AGTACT 1 cut(s) 523
SchI GAGTC 1 cut(s) 273
ScrFI CCNGG 2 cut(s) 361, 374
SduI GDGCHC 2 cut(s) 429, 644
SetI ASST 8 cut(s) 128, 148, 206, 217, 306, 429, 664, 674
SfaNI GCATC 1 cut(s) 316
SinI GGWCC 1 cut(s) 301
SmiMI CAYNNNNRTG 1 cut(s) 512
SmlI CTYRAG 1 cut(s) 450
SmoI CTYRAG 1 cut(s) 450
Sse9I AATT 3 cut(s) 396, 487, 595
SsiI CCGC 3 cut(s) 137, 140, 455
SspMI CTAG 1 cut(s) 440
SstI GAGCTC 1 cut(s) 429
StyD4I CCNGG 2 cut(s) 359, 372
StyI CCWWGG 1 cut(s) 444
TaiI ACGT 1 cut(s) 674
TaqI TCGA 4 cut(s) 184, 367, 593, 606
TasI AATT 3 cut(s) 396, 487, 595
TatI WGTACW 1 cut(s) 521
TauI GCSGC 2 cut(s) 139, 142
TfiI GAWTC 1 cut(s) 311
TscAI CASTG 5 cut(s) 25, 105, 336, 427, 649
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 5 cut(s) 23, 167, 336, 577, 580
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 3 cut(s) 72, 238, 363
TspRI CASTG 5 cut(s) 25, 105, 336, 427, 649
VpaK11BI GGWCC 1 cut(s) 301
XapI RAATTY 1 cut(s) 595
XcmI CCANNNNNNNNNTGG 1 cut(s) 266
XspI CTAG 1 cut(s) 440
ZrmI AGTACT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.