MD01G1130900.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004073
Physical Location & Seq
Reverse (-)
24140343 .. 24141397
1055 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1130900.v1.1.491

Sequence Viewer

Length: 699 bp
ATGGAGTTCGATGCCAAAACTGCTACCACTACTACAAGTACGGCATCACTTCCACCTCCATTCTCCTACTCTCCATCCTCTACTTCTTCTCCCAGTACTGCATCATTTCCCTTTCCTAACTCTCAGCACTTATCTAATTATCTTTCTCCTCATGCTTCTAATAGTAATATTGCTACAAATCCATTTCCGACCTCTCCTTCACCGCCACCGGCACCTGTGATTCTCAGCCCATGCGCCGCCTGCAAGATTCTTCGCCGCAAGTGTGCGGAGAAGTGCGTTTTAGCTCCATACTTCCCTCCTACTGAGCCCCTCAAGTTCACTATTGCCCACAGAGTCTTTGGAGCTAGCAACATCATCAAGCTCTTGCAGGAGATTCCGGAGTCTCATAGAGCAGATGCAGTGAGTAGCATGGTTTACGAAGCCAATGCTAGAATTCACGACCCGGTTTATGGCTGCGCCGGAGCAATTTTCCAACTCCAGAAACAAGTCGGTGAGCTCCAAGCCCAACTGGCCAAGACACAGGCAGAGCTAGTGAACATGCAATGCCAGCAAGGAAATTTGATTGCCCTAATTTGCATGGACATGGCACAGTCTAAAGAACAAGCCATTTTGCAGCAGCAACAGTCTAATAACATTGACACAAGCTGTTTTCTGGATGAAAACAATTTGGGCACGGCTTGGGAGCCTCTCTGGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

233

Amino Acids

25.18

Weight (kDa)

5.93

Isoelectric Point (pI)

71.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 77 - 174 2.6e-40 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 211
AccIII TCCGGA 1 cut(s) 376
AciI CCGC 4 cut(s) 203, 237, 256, 266
AcoI YGGCCR 1 cut(s) 510
AcsI RAATTY 2 cut(s) 432, 556
AfaI GTAC 2 cut(s) 40, 97
AfiI CCNNNNNNNGG 1 cut(s) 449
AluBI AGCT 6 cut(s) 284, 344, 361, 496, 529, 645
AluI AGCT 6 cut(s) 284, 344, 361, 496, 529, 645
Alw21I GWGCWC 1 cut(s) 498
Alw26I GTCTC 1 cut(s) 387
Aor13HI TCCGGA 1 cut(s) 376
AoxI GGCC 1 cut(s) 510
ApeKI GCWGC 3 cut(s) 453, 613, 616
ApoI RAATTY 2 cut(s) 432, 556
AspLEI GCGC 2 cut(s) 236, 458
AsuC2I CCSGG 1 cut(s) 443
AsuHPI GGTGA 2 cut(s) 192, 503
AsuNHI GCTAGC 1 cut(s) 344
BaeGI GKGCMC 1 cut(s) 674
BalI TGGCCA 1 cut(s) 512
BanI GGYRCC 1 cut(s) 211
BanII GRGCYC 2 cut(s) 309, 498
Bbv12I GWGCWC 1 cut(s) 498
BbvI GCAGC 3 cut(s) 440, 625, 628
BccI CCATC 1 cut(s) 82
BceAI ACGGC 2 cut(s) 57, 690
BcgI CGANNNNNNTGC 2 cut(s) 407, 441
BcnI CCSGG 1 cut(s) 443
BcoDI GTCTC 1 cut(s) 387
BfaI CTAG 3 cut(s) 345, 429, 530
BglI GCCNNNNNGGC 1 cut(s) 509
BisI GCNGC 5 cut(s) 237, 256, 454, 614, 617
BlsI GCNGC 5 cut(s) 238, 257, 455, 615, 618
BmcAI AGTACT 1 cut(s) 97
Bme1390I CCNGG 1 cut(s) 443
BmiI GGNNCC 2 cut(s) 213, 684
BmrFI CCNGG 1 cut(s) 443
BmrI ACTGGG 1 cut(s) 87
BmsI GCATC 3 cut(s) 53, 110, 385
BmtI GCTAGC 1 cut(s) 348
BmuI ACTGGG 1 cut(s) 87
BpmI CTGGAG 1 cut(s) 461
BpuEI CTTGAG 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 443
BsaWI WCCGGW 1 cut(s) 376
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bsc4I CCNNNNNNNGG 1 cut(s) 449
Bse118I RCCGGY 1 cut(s) 208
Bse1I ACTGG 2 cut(s) 93, 513
Bse3DI GCAATG 1 cut(s) 548
BseAI TCCGGA 1 cut(s) 376
BseGI GGATG 2 cut(s) 74, 661
BseLI CCNNNNNNNGG 1 cut(s) 449
BseMI GCAATG 1 cut(s) 548
BseMII CTCAG 3 cut(s) 137, 238, 294
BseNI ACTGG 2 cut(s) 93, 513
BseRI GAGGAG 1 cut(s) 138
BseSI GKGCMC 1 cut(s) 674
BseXI GCAGC 3 cut(s) 440, 625, 628
BshFI GGCC 1 cut(s) 512
BshNI GGYRCC 1 cut(s) 211
BsiHKAI GWGCWC 1 cut(s) 498
BsiSI CCGG 4 cut(s) 209, 377, 443, 459
BslI CCNNNNNNNGG 1 cut(s) 449
BsmAI GTCTC 1 cut(s) 387
BsnI GGCC 1 cut(s) 512
Bsp1286I GDGCHC 3 cut(s) 309, 498, 674
Bsp13I TCCGGA 1 cut(s) 376
BspACI CCGC 4 cut(s) 203, 237, 256, 266
BspANI GGCC 1 cut(s) 512
BspCNI CTCAG 3 cut(s) 136, 237, 295
BspEI TCCGGA 1 cut(s) 376
BspLI GGNNCC 2 cut(s) 213, 684
BspOI GCTAGC 1 cut(s) 348
BspT107I GGYRCC 1 cut(s) 211
BsrDI GCAATG 1 cut(s) 548
BsrFI RCCGGY 1 cut(s) 208
BsrI ACTGG 2 cut(s) 93, 513
BssAI RCCGGY 1 cut(s) 208
Bst4CI ACNGT 2 cut(s) 591, 624
BstC8I GCNNGC 3 cut(s) 241, 346, 548
BstDEI CTNAG 3 cut(s) 123, 224, 303
BstF5I GGATG 2 cut(s) 74, 661
BstHHI GCGC 2 cut(s) 236, 458
BstMAI GTCTC 1 cut(s) 387
BstMWI GCNNNNNNNGC 4 cut(s) 20, 240, 509, 547
BstNSI RCATGY 1 cut(s) 541
BstSCI CCNGG 1 cut(s) 441
BstSLI GKGCMC 1 cut(s) 674
BstV1I GCAGC 3 cut(s) 440, 625, 628
BsuRI GGCC 1 cut(s) 512
BtsCI GGATG 2 cut(s) 74, 661
BtsI GCAGTG 1 cut(s) 405
BtsIMutI CAGTG 1 cut(s) 405
Cac8I GCNNGC 3 cut(s) 241, 346, 548
CfoI GCGC 2 cut(s) 236, 458
Cfr10I RCCGGY 1 cut(s) 208
Csp6I GTAC 2 cut(s) 39, 96
CviAII CATG 6 cut(s) 152, 231, 409, 538, 577, 583
CviQI GTAC 2 cut(s) 39, 96
DdeI CTNAG 3 cut(s) 123, 224, 303
EaeI YGGCCR 1 cut(s) 510
Ecl136II GAGCTC 1 cut(s) 496
Eco24I GRGCYC 2 cut(s) 309, 498
Eco53kI GAGCTC 1 cut(s) 496
EcoICRI GAGCTC 1 cut(s) 496
EcoRI GAATTC 1 cut(s) 432
EcoT38I GRGCYC 2 cut(s) 309, 498
FaeI CATG 6 cut(s) 155, 234, 412, 541, 580, 586
FatI CATG 6 cut(s) 151, 230, 408, 537, 576, 582
Fnu4HI GCNGC 5 cut(s) 237, 256, 454, 614, 617
FokI GGATG 2 cut(s) 61, 668
FriOI GRGCYC 2 cut(s) 309, 498
Fsp4HI GCNGC 5 cut(s) 237, 256, 454, 614, 617
FspBI CTAG 3 cut(s) 345, 429, 530
GlaI GCGC 2 cut(s) 235, 457
GluI GCNGC 5 cut(s) 237, 256, 454, 614, 617
GsuI CTGGAG 1 cut(s) 461
HaeIII GGCC 1 cut(s) 512
HapII CCGG 4 cut(s) 209, 377, 443, 459
HhaI GCGC 2 cut(s) 236, 458
Hin1II CATG 6 cut(s) 155, 234, 412, 541, 580, 586
Hin6I GCGC 2 cut(s) 234, 456
HinP1I GCGC 2 cut(s) 234, 456
HinfI GANTC 5 cut(s) 220, 247, 333, 373, 380
HpaII CCGG 4 cut(s) 209, 377, 443, 459
HphI GGTGA 2 cut(s) 192, 503
Hpy166II GTNNAC 3 cut(s) 318, 415, 535
Hpy188I TCNGA 1 cut(s) 189
Hpy188III TCNNGA 5 cut(s) 377, 437, 478, 653, 691
Hpy8I GTNNAC 3 cut(s) 318, 415, 535
HpyAV CCTTC 1 cut(s) 207
HpyCH4III ACNGT 2 cut(s) 591, 624
HpyCH4V TGCA 7 cut(s) 101, 243, 367, 398, 541, 576, 613
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 240, 509, 547
HpyF3I CTNAG 3 cut(s) 123, 224, 303
Hsp92II CATG 6 cut(s) 155, 234, 412, 541, 580, 586
HspAI GCGC 2 cut(s) 234, 456
Kpn2I TCCGGA 1 cut(s) 376
LmnI GCTCC 5 cut(s) 289, 341, 461, 501, 682
Lsp1109I GCAGC 3 cut(s) 440, 625, 628
LweI GCATC 3 cut(s) 53, 110, 385
MaeI CTAG 3 cut(s) 345, 429, 530
MboII GAAGA 2 cut(s) 78, 242
MhlI GDGCHC 3 cut(s) 309, 498, 674
MlsI TGGCCA 1 cut(s) 512
MluCI AATT 6 cut(s) 136, 432, 465, 556, 570, 664
MluNI TGGCCA 1 cut(s) 512
MlyI GAGTC 2 cut(s) 342, 389
MmeI TCCRAC 2 cut(s) 212, 496
MnlI CCTC 7 cut(s) 66, 88, 159, 202, 306, 320, 696
Mox20I TGGCCA 1 cut(s) 512
MroI TCCGGA 1 cut(s) 376
MscI TGGCCA 1 cut(s) 512
MslI CAYNNNNRTG 1 cut(s) 581
Msp20I TGGCCA 1 cut(s) 512
MspI CCGG 4 cut(s) 209, 377, 443, 459
MspR9I CCNGG 1 cut(s) 443
MwoI GCNNNNNNNGC 4 cut(s) 20, 240, 509, 547
NciI CCSGG 1 cut(s) 443
NheI GCTAGC 1 cut(s) 344
NlaIII CATG 6 cut(s) 155, 234, 412, 541, 580, 586
NlaIV GGNNCC 2 cut(s) 213, 684
NspI RCATGY 1 cut(s) 541
PfeI GAWTC 3 cut(s) 220, 247, 373
PkrI GCNGC 5 cut(s) 238, 257, 455, 615, 618
PleI GAGTC 2 cut(s) 341, 388
PpsI GAGTC 2 cut(s) 341, 388
Psp124BI GAGCTC 1 cut(s) 498
PspN4I GGNNCC 2 cut(s) 213, 684
RsaI GTAC 2 cut(s) 40, 97
RsaNI GTAC 2 cut(s) 39, 96
RseI CAYNNNNRTG 1 cut(s) 581
SacI GAGCTC 1 cut(s) 498
SatI GCNGC 5 cut(s) 237, 256, 454, 614, 617
ScaI AGTACT 1 cut(s) 97
SchI GAGTC 2 cut(s) 342, 389
ScrFI CCNGG 1 cut(s) 443
SduI GDGCHC 3 cut(s) 309, 498, 674
SetI ASST 9 cut(s) 58, 194, 217, 286, 346, 363, 498, 531, 647
SfaNI GCATC 3 cut(s) 53, 110, 385
SmiMI CAYNNNNRTG 1 cut(s) 581
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
Sse9I AATT 6 cut(s) 136, 432, 465, 556, 570, 664
SsiI CCGC 4 cut(s) 203, 237, 256, 266
SspI AATATT 1 cut(s) 169
SspMI CTAG 3 cut(s) 345, 429, 530
SstI GAGCTC 1 cut(s) 498
StyD4I CCNGG 1 cut(s) 441
TaaI ACNGT 2 cut(s) 591, 624
TaqI TCGA 1 cut(s) 9
TasI AATT 6 cut(s) 136, 432, 465, 556, 570, 664
TatI WGTACW 1 cut(s) 95
TauI GCSGC 2 cut(s) 239, 258
TfiI GAWTC 3 cut(s) 220, 247, 373
TscAI CASTG 1 cut(s) 405
TseI GCWGC 3 cut(s) 453, 613, 616
TspDTI ATGAA 1 cut(s) 672
TspRI CASTG 1 cut(s) 405
XapI RAATTY 2 cut(s) 432, 556
XceI RCATGY 1 cut(s) 541
XcmI CCANNNNNNNNNTGG 1 cut(s) 335
XspI CTAG 3 cut(s) 345, 429, 530
ZrmI AGTACT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.