AT2G33520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009085
Physical Location & Seq
Forward (+)
14196732 .. 14197706
975 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G33520.1

Sequence Viewer

Length: 294 bp
ATGGCATCTTACCATGTTTCTCATGATTCATATCAATCACCAGGTCCTTCACCATTGTACCAACCGATAATAGAGGCTCCACCGCCACCGTATCCACCAACCCGAACGAGGTATCAAGATTACTACGGTGGCTACGGTCAGCCGCATCCACCACCTCTACGTCCATATAGATCAGATCATGAATACTATGGAGACGGAGAATATGTTGGTTGCTTTCCGTTTCTCAGAAGCTGTTTGACGACGCTATGTTGTTGCTGGTTCGTGGAACAGTGCTGCTTTCGAAGTCGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

97

Amino Acids

11.31

Weight (kDa)

6.38

Isoelectric Point (pI)

85.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 83, 143
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 1 cut(s) 108
AjnI CCWGG 1 cut(s) 40
AluBI AGCT 1 cut(s) 231
AluI AGCT 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 186
AlwNI CAGNNNCTG 1 cut(s) 231
ApeKI GCWGC 1 cut(s) 273
AspS9I GGNCC 1 cut(s) 44
AsuHPI GGTGA 2 cut(s) 30, 42
AsuII TTCGAA 1 cut(s) 280
AvaII GGWCC 1 cut(s) 44
BaeI ACNNNNGTAYC 2 cut(s) 41, 74
BbvI GCAGC 1 cut(s) 260
BciT130I CCWGG 1 cut(s) 42
BciVI GTATCC 1 cut(s) 102
BcoDI GTCTC 1 cut(s) 186
BfuI GTATCC 1 cut(s) 102
BisI GCNGC 2 cut(s) 143, 274
BlsI GCNGC 2 cut(s) 144, 275
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 44
BmiI GGNNCC 1 cut(s) 78
BmrFI CCNGG 1 cut(s) 42
BmsI GCATC 2 cut(s) 14, 154
Bpu14I TTCGAA 1 cut(s) 280
BsaBI GATNNNNATC 1 cut(s) 30
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 1 cut(s) 108
Bse8I GATNNNNATC 1 cut(s) 30
BseBI CCWGG 1 cut(s) 42
BseGI GGATG 1 cut(s) 145
BseJI GATNNNNATC 1 cut(s) 30
BseLI CCNNNNNNNGG 1 cut(s) 108
BseMII CTCAG 1 cut(s) 238
BseXI GCAGC 1 cut(s) 260
BslI CCNNNNNNNGG 1 cut(s) 108
BsmAI GTCTC 1 cut(s) 186
BsmBI CGTCTC 1 cut(s) 186
Bsp119I TTCGAA 1 cut(s) 280
Bsp143I GATC 2 cut(s) 170, 175
BspACI CCGC 2 cut(s) 83, 143
BspCNI CTCAG 1 cut(s) 237
BspHI TCATGA 2 cut(s) 22, 178
BspLI GGNNCC 1 cut(s) 78
BspT104I TTCGAA 1 cut(s) 280
BssMI GATC 2 cut(s) 170, 175
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 4 cut(s) 90, 128, 137, 270
BstBI TTCGAA 1 cut(s) 280
BstDEI CTNAG 1 cut(s) 224
BstF5I GGATG 1 cut(s) 145
BstKTI GATC 2 cut(s) 173, 178
BstMAI GTCTC 1 cut(s) 186
BstMBI GATC 2 cut(s) 170, 175
BstNI CCWGG 1 cut(s) 42
BstSCI CCNGG 1 cut(s) 40
BstV1I GCAGC 1 cut(s) 260
BsuI GTATCC 1 cut(s) 102
BtsCI GGATG 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 275
CaiI CAGNNNCTG 1 cut(s) 231
CciI TCATGA 2 cut(s) 22, 178
Cfr13I GGNCC 1 cut(s) 44
CseI GACGC 1 cut(s) 250
CsiI ACCWGGT 1 cut(s) 40
Csp6I GTAC 1 cut(s) 58
CviAII CATG 3 cut(s) 14, 23, 179
CviJI RGCY 4 cut(s) 77, 132, 142, 231
CviKI_1 RGCY 4 cut(s) 77, 132, 142, 231
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 1 cut(s) 224
DpnI GATC 2 cut(s) 172, 177
DpnII GATC 2 cut(s) 170, 175
Eco47I GGWCC 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 44
EcoRII CCWGG 1 cut(s) 40
Esp3I CGTCTC 1 cut(s) 186
FaeI CATG 3 cut(s) 17, 26, 182
FaiI YATR 9 cut(s) 15, 24, 31, 166, 168, 180, 189, 204, 247
FatI CATG 3 cut(s) 13, 22, 178
Fnu4HI GCNGC 2 cut(s) 143, 274
FokI GGATG 1 cut(s) 132
Fsp4HI GCNGC 2 cut(s) 143, 274
GluI GCNGC 2 cut(s) 143, 274
HgaI GACGC 1 cut(s) 250
Hin1II CATG 3 cut(s) 17, 26, 182
HinfI GANTC 1 cut(s) 26
HphI GGTGA 2 cut(s) 30, 42
Hpy188I TCNGA 2 cut(s) 175, 227
Hpy188III TCNNGA 3 cut(s) 23, 116, 179
Hpy99I CGWCG 1 cut(s) 244
HpyAV CCTTC 1 cut(s) 57
HpyCH4III ACNGT 4 cut(s) 90, 128, 137, 270
HpyCH4IV ACGT 1 cut(s) 160
HpyF3I CTNAG 1 cut(s) 224
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 3 cut(s) 17, 26, 182
Kzo9I GATC 2 cut(s) 170, 175
LmnI GCTCC 1 cut(s) 82
LpnPI CCDG 3 cut(s) 27, 54, 241
Lsp1109I GCAGC 1 cut(s) 260
LweI GCATC 2 cut(s) 14, 154
MabI ACCWGGT 1 cut(s) 40
MaeII ACGT 1 cut(s) 160
MalI GATC 2 cut(s) 172, 177
MboI GATC 2 cut(s) 170, 175
MnlI CCTC 3 cut(s) 67, 102, 165
MseI TTAA 1 cut(s) 292
MspR9I CCNGG 1 cut(s) 42
MvaI CCWGG 1 cut(s) 42
NdeII GATC 2 cut(s) 170, 175
NlaIII CATG 3 cut(s) 17, 26, 182
NlaIV GGNNCC 1 cut(s) 78
NspV TTCGAA 1 cut(s) 280
PagI TCATGA 2 cut(s) 22, 178
PfeI GAWTC 1 cut(s) 26
PkrI GCNGC 2 cut(s) 144, 275
PpuMI RGGWCCY 1 cut(s) 44
Psp5II RGGWCCY 1 cut(s) 44
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 1 cut(s) 78
PspPI GGNCC 1 cut(s) 44
PspPPI RGGWCCY 1 cut(s) 44
PstNI CAGNNNCTG 1 cut(s) 231
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
SaqAI TTAA 1 cut(s) 292
SatI GCNGC 2 cut(s) 143, 274
Sau3AI GATC 2 cut(s) 170, 175
Sau96I GGNCC 1 cut(s) 44
ScrFI CCNGG 1 cut(s) 42
SetI ASST 5 cut(s) 46, 113, 157, 163, 233
SexAI ACCWGGT 1 cut(s) 40
SfaNI GCATC 2 cut(s) 14, 154
SfuI TTCGAA 1 cut(s) 280
SinI GGWCC 1 cut(s) 44
SsiI CCGC 2 cut(s) 83, 143
StyD4I CCNGG 1 cut(s) 40
TaaI ACNGT 4 cut(s) 90, 128, 137, 270
TaiI ACGT 1 cut(s) 163
TaqI TCGA 1 cut(s) 280
TauI GCSGC 1 cut(s) 145
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 1 cut(s) 292
Tru9I TTAA 1 cut(s) 292
TscAI CASTG 1 cut(s) 275
TseI GCWGC 1 cut(s) 273
TspDTI ATGAA 2 cut(s) 18, 195
TspGWI ACGGA 2 cut(s) 207, 210
TspRI CASTG 1 cut(s) 275
VpaK11BI GGWCC 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.