AT2G35040

AICARFT IMPCHase bienzyme family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002841
Physical Location & Seq
Reverse (-)
14765145 .. 14768329
3185 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G35040.1

Sequence Viewer

Length: 1791 bp
ATGCTCAGCTCAGCCGCCACCGCCACCTCCGTGAGTGCTCGCTCCGGCGATATTCTCTGCGGCTTGTTTCGCAAAAAATCCGTCGCGCCTTTTCGTTTTACTCAGCCTGTCTATCGTACGTCGCTGTGTCCAAGTTTCGTTGCTGTTCGAGCCATGGCAGAGTCTCAGACTGCTCAGAGGAATCAGCCGCAATCCTCAGGCTCCTCTGGGGAGAAGCAAGCTTTGATATCTCTATCTGACAAGAGGGACTTGGCTTCTCTTGGCAACGGTCTGCAAGAATTGGGATACACTATTGTTTCTACTGGAGGAACTGCCTCTACTTTGGAGAATGCTGGAGTCTCTGTGACCAAAGTGGAGAAGCTTACTCATTTTCCAGAAATGCTTGATGGCCGTGTGAAAACTTTGCATCCAAACATACATGGTGGTATCCTCGCTAGAAGGGATGTGGAGCATCATATGGAAGCTCTTAATGAGCATGGGATTGGTACATTTGATGTTGTGGTTGTCAATTTGTACCCGTTCTATGAAAAAGTTACTGCTCCAGGTGGGATCAGTTTTGAGGATGGGATTGAGAACATTGACATTGGTGGCCCTGCTATGATTAGAGCAGCTGCTAAGAACCACAAAGACGTTCTCATTGTTGTCGATTCAGGAGATTATCAAGCTGTTTTGGAGTATCTCAAAGGAGGACAGAGCGACCAACAATTCCGCAGAAAACTTGCGTGGAAGGCCTTTCAGCATGTTGCTGCATATGATTCTGCGGTTTCAGAATGGCTATGGAAGCAGACTGAAGGAAAAGAGAAGTTCCCTCCCAGCTTTACAGTTCCGCTTGTACTTAAGAGTTCTCTTCGTTACGGTGAAAATCCTCATCAAAAAGCTGCATTTTATGTTGATAAGAGCCTTGCTGAAGTGAATGCCGGTGGTATCGCAACAGCGATTCAGCATCATGGAAAGGAAATGTCATACAACAACTATCTTGATGCTGACGCTGCATGGAACTGTGTGTCGGAATTTGAAAACCCAACATGTGTAGTCGTGAAGCATACAAATCCTTGTGGTGTGGCCTCTCGTGATGATATTCTTGAGGCTTACCGGCTAGCTGTAAAAGCTGACCCAGTTAGTGCCTTTGGTGGCATTGTAGCTTTCAATGTTGAAGTTGACGAGGTTCTTGCGAGGGAGATCCGAGAGTTCAGGAGCCCAACAGACGGGGAAACTAGAATGTTTTATGAAATCGTGGTTGCTCCAAAGTATACTGCTAAAGGTCTTGAAGTCCTCAAAGGGAAATCAAAAACTTTGAGGATCCTCGAAGCTAAAAAGAATGACCAAGGGAAACTATCGCTCAGACAGGTTGGTGGCGGCTGGTTGGCTCAGGACTCGGACGATTTAACTCCAGAAGACATCAGCTTCAATTCTGTCTCAGACAAAACTCCGACTGAAAGCGAACTTGCGGATGCAAAATTTGCCTGGCTCTGCGTTAAGCATGTCAAGAGCAATGCCATTGTGATAGCAAAGAACAATTGTATGCTGGGGATGGGAAGCGGACAGCCAAACCGAGTAGAGAGTCTGAGAATAGCTTTCAAGAAAGCTGGGGAGGAAGCTAAAGGAGCTGCCTTGGCCAGTGACGCATTCTTCCCATTCGCTTGGAAAGATGCGGTGGAAGAGGCGTGCCAGATGGGAATAGGAGTGATAGCAGAACCTGGAGGCAGTATTAGAGACCAAGACGCTATTGATTGCTGCAAAAAGTATGGAGTCTCTCTGCTCTTCACCAACGTTAGACATTTCCGCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
AICARFT_IMPCHas MGS

Protein Analysis

596

Amino Acids

64.91

Weight (kDa)

6.46

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MGS PF02142 84 - 198 5.6e-22 MGS-like domain
AICARFT_IMPCHas PF01808 204 - 528 6.4e-116 AICARFT/IMPCHase bienzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1250
AccII CGCG 1 cut(s) 86
AclI AACGTT 1 cut(s) 1770
AclWI GGATC 4 cut(s) 557, 1174, 1294, 1307
AcoI YGGCCR 2 cut(s) 388, 1614
AcsI RAATTY 2 cut(s) 1010, 1457
AcuI CTGAAG 2 cut(s) 810, 927
AfaI GTAC 4 cut(s) 118, 487, 515, 834
AfiI CCNNNNNNNGG 1 cut(s) 1205
AflII CTTAAG 1 cut(s) 836
AflIII ACRYGT 1 cut(s) 1025
AgsI TTSAA 6 cut(s) 1016, 1147, 1154, 1268, 1408, 1579
AjnI CCWGG 3 cut(s) 541, 1463, 1696
AleI CACNNNNGTG 1 cut(s) 29
Alw21I GWGCWC 1 cut(s) 40
Alw26I GTCTC 5 cut(s) 168, 343, 1420, 1707, 1756
AlwI GGATC 4 cut(s) 557, 1174, 1294, 1307
AlwNI CAGNNNCTG 1 cut(s) 1697
AoxI GGCC 5 cut(s) 388, 589, 729, 1062, 1614
ApeKI GCWGC 7 cut(s) 608, 611, 746, 878, 989, 1607, 1734
ApoI RAATTY 2 cut(s) 1010, 1457
ArsI GACNNNNNNTTYG 2 cut(s) 66, 98
AspLEI GCGC 1 cut(s) 88
AspS9I GGNCC 1 cut(s) 590
AsuHPI GGTGA 2 cut(s) 869, 1756
AsuNHI GCTAGC 1 cut(s) 1096
AxyI CCTNAGG 1 cut(s) 196
BalI TGGCCA 1 cut(s) 1616
BamHI GGATCC 1 cut(s) 1299
BanII GRGCYC 1 cut(s) 1199
BauI CACGAG 1 cut(s) 1068
BbsI GAAGAC 1 cut(s) 1401
Bbv12I GWGCWC 1 cut(s) 40
BbvI GCAGC 7 cut(s) 598, 620, 733, 865, 976, 1594, 1721
BccI CCATC 4 cut(s) 380, 557, 1525, 1666
BceAI ACGGC 1 cut(s) 375
BcgI CGANNNNNNTGC 2 cut(s) 1151, 1185
BciT130I CCWGG 3 cut(s) 543, 1465, 1698
BciVI GTATCC 2 cut(s) 278, 437
BcoDI GTCTC 5 cut(s) 168, 343, 1420, 1707, 1756
BfaI CTAG 3 cut(s) 435, 1097, 1215
BfrI CTTAAG 1 cut(s) 836
BfuI GTATCC 2 cut(s) 278, 437
BlpI GCTNAGC 2 cut(s) 5, 10
Bme1390I CCNGG 3 cut(s) 543, 1465, 1698
BmgT120I GGNCC 1 cut(s) 590
BmiI GGNNCC 3 cut(s) 202, 1196, 1301
BmrFI CCNGG 3 cut(s) 543, 1465, 1698
BmrI ACTGGG 1 cut(s) 1109
BmsI GCATC 6 cut(s) 415, 460, 952, 970, 1441, 1639
BmtI GCTAGC 1 cut(s) 1100
BmuI ACTGGG 1 cut(s) 1109
BpiI GAAGAC 1 cut(s) 1401
BpmI CTGGAG 5 cut(s) 324, 354, 525, 1374, 1719
Bpu10I CCTNAGC 1 cut(s) 1368
Bpu1102I GCTNAGC 2 cut(s) 5, 10
BpuEI CTTGAG 1 cut(s) 1103
BsaI GGTCTC 1 cut(s) 1707
BsaJI CCNNGG 3 cut(s) 153, 1324, 1611
BsaXI ACNNNNNCTCC 2 cut(s) 327, 357
Bsc4I CCNNNNNNNGG 1 cut(s) 1205
Bse118I RCCGGY 2 cut(s) 917, 1092
Bse1I ACTGG 3 cut(s) 307, 1115, 1617
Bse21I CCTNAGG 1 cut(s) 196
Bse3DI GCAATG 1 cut(s) 1498
BseBI CCWGG 3 cut(s) 543, 1465, 1698
BseDI CCNNGG 3 cut(s) 153, 1324, 1611
BseGI GGATG 5 cut(s) 406, 448, 568, 1456, 1536
BseLI CCNNNNNNNGG 1 cut(s) 1205
BseMI GCAATG 1 cut(s) 1498
BseNI ACTGG 3 cut(s) 307, 1115, 1617
BseRI GAGGAG 1 cut(s) 193
BseXI GCAGC 7 cut(s) 598, 620, 733, 865, 976, 1594, 1721
BseYI CCCAGC 3 cut(s) 812, 1525, 1586
Bsh1236I CGCG 1 cut(s) 86
BshFI GGCC 5 cut(s) 390, 591, 731, 1064, 1616
BsiHKAI GWGCWC 1 cut(s) 40
BsiSI CCGG 3 cut(s) 45, 918, 1093
BsiWI CGTACG 1 cut(s) 116
BslFI GGGAC 1 cut(s) 260
BslI CCNNNNNNNGG 1 cut(s) 1205
BsmAI GTCTC 5 cut(s) 168, 343, 1420, 1707, 1756
BsmFI GGGAC 1 cut(s) 260
BsmI GAATGC 3 cut(s) 334, 919, 1625
BsnI GGCC 5 cut(s) 390, 591, 731, 1064, 1616
Bso31I GGTCTC 1 cut(s) 1707
Bsp1286I GDGCHC 2 cut(s) 40, 1199
Bsp143I GATC 3 cut(s) 549, 1179, 1299
Bsp1720I GCTNAGC 2 cut(s) 5, 10
Bsp19I CCATGG 1 cut(s) 153
BspANI GGCC 5 cut(s) 390, 591, 731, 1064, 1616
BspFNI CGCG 1 cut(s) 86
BspLI GGNNCC 3 cut(s) 202, 1196, 1301
BspOI GCTAGC 1 cut(s) 1100
BspPI GGATC 4 cut(s) 557, 1174, 1294, 1307
BspQI GCTCTTC 1 cut(s) 1766
BspTI CTTAAG 1 cut(s) 836
BspTNI GGTCTC 1 cut(s) 1707
BsrDI GCAATG 1 cut(s) 1498
BsrFI RCCGGY 2 cut(s) 917, 1092
BsrI ACTGG 3 cut(s) 307, 1115, 1617
BssAI RCCGGY 2 cut(s) 917, 1092
BssECI CCNNGG 3 cut(s) 153, 1324, 1611
BssMI GATC 3 cut(s) 549, 1179, 1299
BssNAI GTATAC 1 cut(s) 1251
BssSI CACGAG 1 cut(s) 1068
BssT1I CCWWGG 3 cut(s) 153, 1324, 1611
Bst1107I GTATAC 1 cut(s) 1251
Bst2BI CACGAG 1 cut(s) 1068
Bst2UI CCWGG 3 cut(s) 543, 1465, 1698
Bst4CI ACNGT 4 cut(s) 269, 823, 857, 1001
Bst6I CTCTTC 3 cut(s) 852, 1653, 1766
BstAFI CTTAAG 1 cut(s) 836
BstAPI GCANNNNNTGC 1 cut(s) 1460
BstC8I GCNNGC 4 cut(s) 40, 219, 1098, 1666
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 5 cut(s) 406, 448, 568, 1456, 1536
BstFNI CGCG 1 cut(s) 86
BstHHI GCGC 1 cut(s) 88
BstKTI GATC 3 cut(s) 552, 1182, 1302
BstMAI GTCTC 5 cut(s) 168, 343, 1420, 1707, 1756
BstMBI GATC 3 cut(s) 549, 1179, 1299
BstNI CCWGG 3 cut(s) 543, 1465, 1698
BstNSI RCATGY 3 cut(s) 743, 1029, 1484
BstSCI CCNGG 3 cut(s) 541, 1463, 1696
BstUI CGCG 1 cut(s) 86
BstV1I GCAGC 7 cut(s) 598, 620, 733, 865, 976, 1594, 1721
BstV2I GAAGAC 1 cut(s) 1401
BstX2I RGATCY 2 cut(s) 1179, 1299
BstXI CCANNNNNNTGG 1 cut(s) 1641
BstYI RGATCY 2 cut(s) 1179, 1299
BstZ17I GTATAC 1 cut(s) 1251
Bsu36I CCTNAGG 1 cut(s) 196
BsuI GTATCC 2 cut(s) 278, 437
BsuRI GGCC 5 cut(s) 390, 591, 731, 1064, 1616
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 5 cut(s) 406, 448, 568, 1456, 1536
BtsIMutI CAGTG 1 cut(s) 1624
Cac8I GCNNGC 4 cut(s) 40, 219, 1098, 1666
CaiI CAGNNNCTG 1 cut(s) 1697
CfoI GCGC 1 cut(s) 88
Cfr10I RCCGGY 2 cut(s) 917, 1092
Cfr13I GGNCC 1 cut(s) 590
CseI GACGC 3 cut(s) 995, 1631, 1730
Csp6I GTAC 4 cut(s) 117, 486, 514, 833
CviAII CATG 8 cut(s) 154, 419, 476, 740, 947, 993, 1026, 1481
CviQI GTAC 4 cut(s) 117, 486, 514, 833
DpnI GATC 3 cut(s) 551, 1181, 1301
DpnII GATC 3 cut(s) 549, 1179, 1299
EaeI YGGCCR 2 cut(s) 388, 1614
Eam1104I CTCTTC 3 cut(s) 852, 1653, 1766
EarI CTCTTC 3 cut(s) 852, 1653, 1766
EciI GGCGGA 1 cut(s) 1772
Eco130I CCWWGG 3 cut(s) 153, 1324, 1611
Eco147I AGGCCT 1 cut(s) 731
Eco24I GRGCYC 1 cut(s) 1199
Eco31I GGTCTC 1 cut(s) 1707
Eco32I GATATC 1 cut(s) 228
Eco57I CTGAAG 2 cut(s) 810, 927
Eco81I CCTNAGG 1 cut(s) 196
EcoRII CCWGG 3 cut(s) 541, 1463, 1696
EcoRV GATATC 1 cut(s) 228
EcoT14I CCWWGG 3 cut(s) 153, 1324, 1611
EcoT38I GRGCYC 1 cut(s) 1199
ErhI CCWWGG 3 cut(s) 153, 1324, 1611
FaeI CATG 8 cut(s) 157, 422, 479, 743, 950, 996, 1029, 1484
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FaqI GGGAC 1 cut(s) 260
FatI CATG 8 cut(s) 153, 418, 475, 739, 946, 992, 1025, 1480
FauNDI CATATG 2 cut(s) 456, 751
FblI GTMKAC 1 cut(s) 1250
FokI GGATG 5 cut(s) 393, 455, 575, 1463, 1543
FriOI GRGCYC 1 cut(s) 1199
FspBI CTAG 3 cut(s) 435, 1097, 1215
GlaI GCGC 1 cut(s) 87
GsaI CCCAGC 3 cut(s) 816, 1529, 1590
GsuI CTGGAG 5 cut(s) 324, 354, 525, 1374, 1719
HaeIII GGCC 5 cut(s) 390, 591, 731, 1064, 1616
HapII CCGG 3 cut(s) 45, 918, 1093
HgaI GACGC 3 cut(s) 995, 1631, 1730
HhaI GCGC 1 cut(s) 88
Hin1II CATG 8 cut(s) 157, 422, 479, 743, 950, 996, 1029, 1484
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HincII GTYRAC 1 cut(s) 1159
HindII GTYRAC 1 cut(s) 1159
HindIII AAGCTT 2 cut(s) 219, 359
HinfI GANTC 9 cut(s) 161, 181, 336, 647, 755, 937, 1373, 1561, 1749
HpaII CCGG 3 cut(s) 45, 918, 1093
HphI GGTGA 2 cut(s) 869, 1756
Hpy166II GTNNAC 2 cut(s) 1159, 1251
Hpy8I GTNNAC 2 cut(s) 1159, 1251
Hpy99I CGWCG 2 cut(s) 86, 124
HpyAV CCTTC 3 cut(s) 432, 721, 785
HpyCH4III ACNGT 4 cut(s) 269, 823, 857, 1001
HpyCH4IV ACGT 3 cut(s) 119, 630, 1770
HpyCH4V TGCA 7 cut(s) 274, 406, 749, 881, 992, 1454, 1737
HpySE526I ACGT 3 cut(s) 119, 630, 1770
Hsp92II CATG 8 cut(s) 157, 422, 479, 743, 950, 996, 1029, 1484
HspAI GCGC 1 cut(s) 86
Kzo9I GATC 3 cut(s) 549, 1179, 1299
LguI GCTCTTC 1 cut(s) 1766
LmnI GCTCC 7 cut(s) 47, 206, 448, 544, 1194, 1246, 1604
Lsp1109I GCAGC 7 cut(s) 598, 620, 733, 865, 976, 1594, 1721
LweI GCATC 6 cut(s) 415, 460, 952, 970, 1441, 1639
MaeI CTAG 3 cut(s) 435, 1097, 1215
MaeII ACGT 3 cut(s) 119, 630, 1770
MaeIII GTNAC 4 cut(s) 343, 532, 851, 1619
MalI GATC 3 cut(s) 551, 1181, 1301
MboI GATC 3 cut(s) 549, 1179, 1299
MboII GAAGA 5 cut(s) 839, 1406, 1621, 1670, 1753
MfeI CAATTG 1 cut(s) 1516
MflI RGATCY 2 cut(s) 1179, 1299
MhlI GDGCHC 2 cut(s) 40, 1199
MlsI TGGCCA 1 cut(s) 1616
MluCI AATT 7 cut(s) 278, 508, 704, 1010, 1408, 1457, 1516
MluNI TGGCCA 1 cut(s) 1616
MlyI GAGTC 5 cut(s) 170, 345, 1367, 1570, 1758
MmeI TCCRAC 2 cut(s) 987, 1454
Mox20I TGGCCA 1 cut(s) 1616
MscI TGGCCA 1 cut(s) 1616
MseI TTAA 5 cut(s) 468, 837, 1385, 1476, 1789
MslI CAYNNNNRTG 1 cut(s) 29
Msp20I TGGCCA 1 cut(s) 1616
MspA1I CMGCKG 1 cut(s) 611
MspCI CTTAAG 1 cut(s) 836
MspI CCGG 3 cut(s) 45, 918, 1093
MspR9I CCNGG 3 cut(s) 543, 1465, 1698
MunI CAATTG 1 cut(s) 1516
Mva1269I GAATGC 3 cut(s) 334, 919, 1625
MvaI CCWGG 3 cut(s) 543, 1465, 1698
MvnI CGCG 1 cut(s) 86
NcoI CCATGG 1 cut(s) 153
NdeI CATATG 2 cut(s) 456, 751
NdeII GATC 3 cut(s) 549, 1179, 1299
NheI GCTAGC 1 cut(s) 1096
NlaIII CATG 8 cut(s) 157, 422, 479, 743, 950, 996, 1029, 1484
NlaIV GGNNCC 3 cut(s) 202, 1196, 1301
NmuCI GTSAC 2 cut(s) 343, 1619
NspI RCATGY 3 cut(s) 743, 1029, 1484
OliI CACNNNNGTG 1 cut(s) 29
PceI AGGCCT 1 cut(s) 731
PciI ACATGT 1 cut(s) 1025
PciSI GCTCTTC 1 cut(s) 1766
PctI GAATGC 3 cut(s) 334, 919, 1625
PfeI GAWTC 4 cut(s) 181, 647, 755, 937
Pfl23II CGTACG 1 cut(s) 116
PleI GAGTC 5 cut(s) 169, 344, 1367, 1569, 1757
PpsI GAGTC 5 cut(s) 169, 344, 1367, 1569, 1757
PscI ACATGT 1 cut(s) 1025
Psp1406I AACGTT 1 cut(s) 1770
Psp6I CCWGG 3 cut(s) 541, 1463, 1696
PspFI CCCAGC 3 cut(s) 812, 1525, 1586
PspGI CCWGG 3 cut(s) 541, 1463, 1696
PspLI CGTACG 1 cut(s) 116
PspN4I GGNNCC 3 cut(s) 202, 1196, 1301
PspPI GGNCC 1 cut(s) 590
PsrI GAACNNNNNNTAC 2 cut(s) 301, 333
PstNI CAGNNNCTG 1 cut(s) 1697
PsuI RGATCY 2 cut(s) 1179, 1299
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 4 cut(s) 118, 487, 515, 834
RsaNI GTAC 4 cut(s) 117, 486, 514, 833
RseI CAYNNNNRTG 1 cut(s) 29
SapI GCTCTTC 1 cut(s) 1766
SaqAI TTAA 5 cut(s) 468, 837, 1385, 1476, 1789
Sau3AI GATC 3 cut(s) 549, 1179, 1299
Sau96I GGNCC 1 cut(s) 590
SchI GAGTC 5 cut(s) 170, 345, 1367, 1570, 1758
ScrFI CCNGG 3 cut(s) 543, 1465, 1698
SduI GDGCHC 2 cut(s) 40, 1199
SfaNI GCATC 6 cut(s) 415, 460, 952, 970, 1441, 1639
SmiMI CAYNNNNRTG 1 cut(s) 29
SmlI CTYRAG 2 cut(s) 836, 1082
SmoI CTYRAG 2 cut(s) 836, 1082
Sse9I AATT 7 cut(s) 278, 508, 704, 1010, 1408, 1457, 1516
SseBI AGGCCT 1 cut(s) 731
SspMI CTAG 3 cut(s) 435, 1097, 1215
StuI AGGCCT 1 cut(s) 731
StyD4I CCNGG 3 cut(s) 541, 1463, 1696
StyI CCWWGG 3 cut(s) 153, 1324, 1611
TaaI ACNGT 4 cut(s) 269, 823, 857, 1001
TaiI ACGT 3 cut(s) 122, 633, 1773
TaqI TCGA 3 cut(s) 148, 645, 1305
TasI AATT 7 cut(s) 278, 508, 704, 1010, 1408, 1457, 1516
TatI WGTACW 1 cut(s) 832
TauI GCSGC 4 cut(s) 17, 63, 190, 1359
TfiI GAWTC 4 cut(s) 181, 647, 755, 937
Tru1I TTAA 5 cut(s) 468, 837, 1385, 1476, 1789
Tru9I TTAA 5 cut(s) 468, 837, 1385, 1476, 1789
TscAI CASTG 1 cut(s) 1624
TseFI GTSAC 2 cut(s) 343, 1619
TseI GCWGC 7 cut(s) 608, 611, 746, 878, 989, 1607, 1734
Tsp45I GTSAC 2 cut(s) 343, 1619
TspDTI ATGAA 2 cut(s) 540, 1242
TspGWI ACGGA 2 cut(s) 19, 70
TspRI CASTG 1 cut(s) 1624
Vha464I CTTAAG 1 cut(s) 836
XapI RAATTY 2 cut(s) 1010, 1457
XceI RCATGY 3 cut(s) 743, 1029, 1484
XmiI GTMKAC 1 cut(s) 1250
XspI CTAG 3 cut(s) 435, 1097, 1215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.